CN107266548A - The method that a kind of utilization ionic liquid and protease extract tussah silk fibroin albumen - Google Patents

The method that a kind of utilization ionic liquid and protease extract tussah silk fibroin albumen Download PDF

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Publication number
CN107266548A
CN107266548A CN201710626825.8A CN201710626825A CN107266548A CN 107266548 A CN107266548 A CN 107266548A CN 201710626825 A CN201710626825 A CN 201710626825A CN 107266548 A CN107266548 A CN 107266548A
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ionic liquid
fibroin albumen
fibroin
tussah silk
protease
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Inventor
胡智文
陈茹茹
李津
梁军龙
徐城锋
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Zhejiang Sci Tech University ZSTU
Zhejiang University of Science and Technology ZUST
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Zhejiang Sci Tech University ZSTU
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Abstract

Field is extracted the present invention relates to fibroin albumen, a kind of method that utilization ionic liquid and protease extract tussah silk fibroin albumen is disclosed, tussah silk is handled using alkaline process degumming, fibroin fiber is obtained；Fibroin fiber is mixed with previously prepared [AMIM] Cl ionic liquids, the agitating and heating certain time in oil bath, preliminarily solubilised is carried out to tussah silk, PM13 alkali proteases are added to be digested, fibroin albumen is progressively dissolved using ionic liquid and PM13 alkali proteases, by add absolute ethyl alcohol remove ionic liquid, so as to get fibroin albumen dissolve again in deionized water, by dialysing and being freeze-dried to obtain pure silk fibroin powder.The extracting method of this tussah silk fibroin albumen has that reaction condition is gentle, chemical levels are few, ionic liquid is recyclable, and tussah silk fibroin albumen effectively can be dissolved.

Description

The method that a kind of utilization ionic liquid and protease extract tussah silk fibroin albumen
Technical field
Field is extracted the present invention relates to fibroin albumen, more particularly to one kind extracts tussah silk using ionic liquid and protease The method of fibroin albumen.
Background technology
Silk is mainly made up of fibroin albumen and sericin, and wherein fibroin albumen accounts for 70%, be silk it is main into Point.Silk gum is globular preteins, contains a large amount of polar hydrophilic side bases in composition amino acid, stability is poor, dissolves in acid, alkali, albumen In the solution such as enzyme and low concentration sodium carbonate；And fibroin is fibrous protein, natural fibroin fiber contains a large amount of intramoleculars and divided Hydrogen bond between son, crystallinity is dissolved in common solvent compared with Gao Ernan.
Relatively common in life is mulberry silk and tussah silk, mainly by glycine（Gly）, alanine（Ala）, serine （Ser）And tyrosine（Tyr）Composition, but amino acid content and distributional difference are larger.In tussah silk peptide, with reactive group Amino acid（Such as arginine, aspartic acid）More than mulberry silk, tryptophan, the content of histidine are 5 times of mulberry silk, tussah silk Alanine content be 40%, mulberry silk is 30%.Therefore the bending of peptide chain and entanglement degree ratio mulberry silk are big in tussah silk, this just makes Rigidity, the solvent resistant ability of tussah silk are higher than mulberry silk.
At present, in research process, frequently with strong acid, highly basic, high concentration salting liquid such as high concentration CaCl2Solution, copper Ammonia solution, lithium-bromide solution, thiocyanation lithium solution etc. dissolve to Bombyx silk albumen, but these solvents are present to albumen Matter molecular degradation is serious, toxic, unstable, the shortcomings of be not easily recycled, at the same to the extraction effect of tussah silk fibroin albumen compared with Difference.
Ionic liquid (IL) is the green solvent of the great application prospect of a class of rising in recent years, is widely used in electrochemistry , organic synthesis, chemical separating, material prepare etc. field.So-called ionic liquid is exactly in room temperature（Or a little higher than room temperature Temperature）Under the ionic system that is in a liquid state, it is different from common are machine solvent, there is powerful electrostatic phase interaction in ionic liquid With, therefore show unusual feature：Non-volatile, high stability, good conduction and thermal conductivity, selective dissolution power with Designability, while having characteristic environmentally friendly, can be recycled.
The content of the invention
In order to solve the above-mentioned technical problem, tussah silk silk is extracted using ionic liquid and protease the invention provides one kind The method of fibroin.The extracting method of the present invention is more gentle, damages small to fibroin albumen, nonhazardous environmentally friendly using raw material, and Extraction efficiency is higher.
The present invention concrete technical scheme be：A kind of utilization ionic liquid and protease extract the side of tussah silk fibroin albumen Method, in terms of g and mL, comprises the following steps：
1）3.5-4.5g tussah silks are weighed, are cleaned with deionized water, surface contaminant, drying is removed.
2）By the tussah silk of drying with 1:95-105 bath raio is in the Na containing 0.4-0.6wt%2PO4With 0.8-1.2wt%'s C17H3525-35min is boiled in COONa mixed solution, degumming process is carried out, altogether degumming four times.
In scouring processes, with the hydrolysis of silk gum, the concentration of amino acid gradually increases in solution, pH reductions, degumming efficiency Weaken, so alkali need to be added to maintain the stabilization of degumming liquid pH value, but alkali concn crosses conference damage fibroin, so using C17H35COONa is used as buffer.
3）After degumming, tussah silk is washed by rubbing with the hands more than 4 times with deionized water, 55-65 DEG C of baking oven is put into, obtain the silk of drying Cellulose fiber.
4）The fibroin fiber of 1.8-2.2g dryings is weighed, with 1:In 45-55 bath raio immersion [AMIM] Cl ionic liquids, control Reaction temperature processed stirs 5-7h in the range of 80 ~ 100 DEG C in oil bath, after the completion of reaction, is cooled to room temperature.
5）To step 4）PM is added in intermediate ion liquid13- basic protein enzyme powder 0.25-0.35g, in 40 ~ 55 DEG C of oil 5-7h is stirred in bath, is obtained after fibroin/ionic liquid solution, the enzyme that goes out is carried out.
Biology enzyme is a kind of nontoxic and environment-friendly catalyst, possesses efficient specificity；And consumption is few, water Solution condition（Temperature, pH）Gently, pollution is reduced, is economized on resources.Ionic liquid (IL) is the great application of a class of rising in recent years The green solvent of prospect, certain dissolubility is shown to fibroin albumen.
The present invention uses PM13Alkali protease is modified, to keep stabilization of the alkali protease in ionic liquid Property and activity.Alkali protease is also a kind of protein in itself, and ionic liquid has certain destruction, PM to its structure13 Place is a kind of comb copolymer, can be covered in alkali protease surface, prevent alkali protease from being destroyed by solion, simultaneously By compatibility of the PM13 chains to IL, the stability of enzyme can be not only improved, it is ensured that activity of the enzyme in ionic liquid, and make Enzyme is uniformly dispersed in IL.
In addition, the amino acid composition of tussah silk and mulberry silk has significant differences, although alanine and sweet ammonia in two kinds of silks The content of content alanine all in 75% or so, but tussah silk of acid is more than glycine, and mulberry silk is then on the contrary, this causes On peptide chain structure, tussah silk is based on the peptide chain of propyl- third, and the methyl in propyl- the third peptide chain structure side base so that the shape between side base Into intensive hydrophobic effect, tussah silk is set to be difficult to dissolve.Simultaneously as the adhesion of tussah silk crystal region is more than mulberry silk, lead Cause its dissolubility poor.Therefore, during being dissolved to it, first with solvability of the ionic liquid to fibroin albumen, in height Preliminarily solubilised is carried out to fibroin albumen under the conditions of temperature, PM is added13- alkali protease, while using ion liquid dissolving Hydrolysis and efficiency of the enzyme to fibroin albumen can also be improved.
The present invention uses PM13Alkali protease is modified, to keep stabilization of the alkali protease in ionic liquid Property and activity.Alkali protease is also a kind of protein in itself, and ionic liquid has certain destruction, PM to its structure13 Place is a kind of comb copolymer, can be covered in alkali protease surface, prevent alkali protease from being destroyed by solion, simultaneously By compatibility of the PM13 chains to IL, the stability of enzyme can be not only improved, it is ensured that activity of the enzyme in ionic liquid, and make Enzyme is uniformly dispersed in IL.
6）Treat that fibroin/ionic liquid solution is cooled to room temperature, add absolute ethyl alcohol, soak repeatedly, separate out fibroin albumen, Mixture is filtered by vacuum, deionized water is added into the fibroin albumen filtered out, is filtered after soaking repeatedly, then solution is filled Enter the 20-28h that dialysed in bag filter, obtain pure silk fibroin protein solution.
7）Obtained silk fibroin protein solution is freeze-dried, you can obtain silk fibroin powder.
Preferably, step 4）In, the preparation method of [AMIM] the Cl ionic liquids is：By 1.0-1.5:1 mole Than adding allyl chloride and N- methylimidazoles into reaction vessel, 6 ~ 8h is stirred at reflux in 55-65 DEG C of oil bath, reaction is finished Afterwards, excessive 2- methallyl chlorides are removed using rotary evaporation, light yellow clear liquid are obtained, i.e. [AMIM] Cl ionic liquids After body, freeze-drying 20-28h, thaw stand-by.
In the above-mentioned methods, it is necessary to which obtained product is freeze-dried, water that may be present in ionic liquid is removed Point.Water is polar solvent, brings part hydrogen bond into, and ionic liquid is had an effect, so that ionic liquid can be reduced to fibroin albumen Dissolution.
Preferably, step 5）In, the PM13The preparation method of-alkali protease is：To 90-110mL 0.1M boron 0.10-0.20g alkali proteases and 3.5-4.0g PM are added in sour sodium cushioning liquid13, it is slowly stirred under the conditions of 1-5 DEG C 0.5-1.5h, ultrafiltration removes unreacted PM under conditions of additional 45-55mL 1mmol/L HCl13, in triplicate, by filtrate It is freeze-dried, obtains PM13- basic protein enzyme powder, it is stand-by.
Preferably, the PM13For MW15000 pectinations PEG.
Preferably, in PM13It is cold under the conditions of -20 DEG C in advance before freeze-drying in the preparation process of-alkali protease Freeze 4h.
Preferably, step 5）In, go out enzyme when, at least 30min is incubated in the oil bath more than 80 DEG C.
Preferably, step 6）In, the molecular cut off of the bag filter is 3500.
It is compared with the prior art, the beneficial effects of the invention are as follows：
（1）The present invention utilizes PM13Alkali protease is modified, PM is utilized13To the affinity interaction of ionic liquid, not only carry The high stability and reactivity of enzyme, while adding dispersing uniformity of the enzyme in ionic liquid, is conducive to it to fibroin The hydrolysis of albumen.
（2）The present invention adds C in scouring processes17H35COONa maintains alkaline environment as buffer, improves degumming Efficiency, while reducing the injury to fibroin fiber.
（3）The present invention is progressively handled fibroin albumen, carried using the dual dissolution effect of ionic liquid and biology enzyme The high solubility of fibroin albumen.
（4）In the present invention, biology enzyme is nontoxic, with it is environment-friendly, while consumption is few, economize on resources；It is specific high, make With mild condition, the destruction to fibroin albumen is small.Environmentally friendly and ionic liquid is " green solvent ", group can be designed, and It is easily recycled, can be recycled.
Embodiment
With reference to embodiment, the invention will be further described.
Embodiment 1：
The method that a kind of utilization ionic liquid and protease extract tussah silk fibroin albumen, comprises the following steps：
1）4g tussah silks are weighed as sample, are cleaned with deionized water, surface contaminant, drying is removed.
2）By the sample of drying with 1:100 bath raio is containing 0.5% Na2PO4And 1%C17H35In COONa mixed solution 30min is boiled, degumming process is carried out, altogether degumming four times.
3）After degumming, sample is washed by rubbing with the hands more than 4 times with deionized water, 60 DEG C of baking ovens are put into, dry fibroin is obtained fine Dimension.
4）The fibroin fiber of 2g dryings is weighed, with 1:In 50 bath raio immersion [AMIM] Cl ionic liquids, control reaction temperature Degree stirs 6h in the range of 80 DEG C in oil bath.After the completion of reaction, room temperature is cooled to.
5）To 4）PM is added in intermediate ion liquid13- basic protein enzyme powder 0.3g, stirs 6h in 40 DEG C of oil bath, obtains To after fibroin/ionic liquid solution, the enzyme that goes out is carried out.
6）Treat that fibroin/ionic liquid solution is cooled to room temperature, add absolute ethyl alcohol, soak repeatedly, have fibroin albumen analysis Go out.Mixture is filtered by vacuum, deionized water is added into the fibroin albumen filtered out, is filtered after soaking repeatedly, then will be molten Liquid is fitted into the bag filter of molecular cut off 3500 24h that dialyses, and obtains pure silk fibroin protein solution.
7）Obtained silk fibroin protein solution is freeze-dried, you can obtain silk fibroin powder.
Wherein, PM13The preparation of-alkali protease：0.15g alkali is added into 100mL 0.1M sodium borate buffer solution Property protease and 3.75g PM13（Pectination PEG MW15000）, 1h is slowly stirred under the conditions of 4 DEG C, in additional 50mL 1mmol/L Ultrafiltration removes unreacted PM under conditions of HCl13, in triplicate.Filtrate is freeze-dried, PM is obtained13- basic protein Enzyme powder, it is stand-by.
The preparation of [AMIM] Cl ionic liquids：According to 1.25:1 mol ratio added into three-necked flask allyl chloride and N- methylimidazoles, are stirred at reflux 6h in 60 DEG C of oil baths, after completion of the reaction, and excessive 2- methallyls are removed using rotary evaporation Base chlorine, obtains light yellow clear liquid, i.e. [AMIM] Cl ionic liquids, after freeze-drying 24h, thaws stand-by.
Embodiment 2：
The method that a kind of utilization ionic liquid and protease extract tussah silk fibroin albumen, comprises the following steps：
1）4g tussah silks are weighed as sample, are cleaned with deionized water, surface contaminant, drying is removed.
2）By the sample of drying with 1:100 bath raio is containing 0.5% Na2PO4And 1%C17H35In COONa mixed solution 30min is boiled, degumming process is carried out, altogether degumming four times.
3）After degumming, sample is washed by rubbing with the hands more than 4 times with deionized water, 60 DEG C of baking ovens are put into, dry fibroin is obtained fine Dimension.
4）The fibroin fiber of 2g dryings is weighed, with 1:In 50 bath raio immersion [AMIM] Cl ionic liquids, control reaction temperature Degree stirs 6h in the range of 90 DEG C in oil bath.After the completion of reaction, room temperature is cooled to.
5）To 4）PM is added in intermediate ion liquid13- basic protein enzyme powder 0.3g, stirs 6h in 47 DEG C of oil bath, obtains To after fibroin/ionic liquid solution, the enzyme that goes out is carried out.
6）Treat that fibroin/ionic liquid solution is cooled to room temperature, add absolute ethyl alcohol, soak repeatedly, have fibroin albumen analysis Go out.Mixture is filtered by vacuum, deionized water is added into the fibroin albumen filtered out, is filtered after soaking repeatedly, then will be molten Liquid is fitted into the bag filter of molecular cut off 3500 24h that dialyses, and obtains pure silk fibroin protein solution.
7）Obtained silk fibroin protein solution is freeze-dried, you can obtain silk fibroin powder.
Wherein, PM13The preparation of-alkali protease：0.15g alkali is added into 100mL 0.1M sodium borate buffer solution Property protease and 3.75g PM13（Pectination PEG MW15000）, 1h is slowly stirred under the conditions of 4 DEG C, in additional 50mL 1mmol/L Ultrafiltration removes unreacted PM under conditions of HCl13, in triplicate.Filtrate is freeze-dried, PM is obtained13- basic protein Enzyme powder, it is stand-by.
The preparation of [AMIM] Cl ionic liquids：According to 1.25:1 mol ratio adds allyl chloride and N- into three-necked flask Methylimidazole, is stirred at reflux 7h in 60 DEG C of oil baths, after completion of the reaction, and excessive 2- methacrylics are removed using rotary evaporation Chlorine, obtains light yellow clear liquid, i.e. [AMIM] Cl ionic liquids, after freeze-drying 24h, thaws stand-by.
Embodiment 3：
The method that a kind of utilization ionic liquid and protease extract tussah silk fibroin albumen, comprises the following steps：
1）4g tussah silks are weighed as sample, are cleaned with deionized water, surface contaminant, drying is removed.
2）By the sample of drying with 1:100 bath raio is containing 0.5% Na2PO4And 1%C17H35In COONa mixed solution 30min is boiled, degumming process is carried out, altogether degumming four times.
3）After degumming, sample is washed by rubbing with the hands more than 4 times with deionized water, 60 DEG C of baking ovens are put into, dry fibroin is obtained fine Dimension.
4）The fibroin fiber of 2g dryings is weighed, with 1:In 50 bath raio immersion [AMIM] Cl ionic liquids, control reaction temperature Degree stirs 6h in the range of 100 DEG C in oil bath.After the completion of reaction, room temperature is cooled to.
5）To 4）PM is added in intermediate ion liquid13- basic protein enzyme powder 0.3g, stirs 6h in 55 DEG C of oil bath, obtains To after fibroin/ionic liquid solution, the enzyme that goes out is carried out.
6）Treat that fibroin/ionic liquid solution is cooled to room temperature, add absolute ethyl alcohol, soak repeatedly, have fibroin albumen analysis Go out.Mixture is filtered by vacuum, deionized water is added into the fibroin albumen filtered out, is filtered after soaking repeatedly, then will be molten Liquid is fitted into the bag filter of molecular cut off 3500 24h that dialyses, and obtains pure silk fibroin protein solution.
7）Obtained silk fibroin protein solution is freeze-dried, you can obtain silk fibroin powder.
Wherein, PM13The preparation of-alkali protease：0.15g alkali is added into 100mL 0.1M sodium borate buffer solution Property protease and 3.75g PM13（Pectination PEG MW15000）, 1h is slowly stirred under the conditions of 4 DEG C, in additional 50mL 1mmol/L Ultrafiltration removes unreacted PM under conditions of HCl13, in triplicate.Filtrate is freeze-dried, PM is obtained13- basic protein Enzyme powder, it is stand-by.
The preparation of [AMIM] Cl ionic liquids：According to 1.25:1 mol ratio added into three-necked flask allyl chloride and N- methylimidazoles, are stirred at reflux 8h in 60 DEG C of oil baths, after completion of the reaction, and excessive 2- methallyls are removed using rotary evaporation Base chlorine, obtains light yellow clear liquid, i.e. [AMIM] Cl ionic liquids, after freeze-drying 24h, thaws stand-by.
Contrast experiment：
A kind of method of enzymolysis and extraction fibroin albumen single in aqueous solvent, step is as follows：
1）4g tussah silks are weighed as sample, are cleaned with deionized water, surface contaminant, drying is removed.
2）By the sample of drying with 1:100 bath raio is containing 0.5% Na2PO4And 1%C17H35In COONa mixed solution 30min is boiled, degumming process is carried out, altogether degumming four times.
3）After degumming, sample is washed by rubbing with the hands more than 4 times with deionized water, 60 DEG C of baking ovens are put into, dry fibroin is obtained fine Dimension.
4）2g fibroin fibers are weighed, with 1:50 bath raio immersion CB buffer solutions（Na2CO3/NaHCO3Buffer solution）In, regulation PH value of solution is slowly stirred 24h under the conditions of 40 ~ 55 DEG C of oil bath, after the completion of reaction, need to carry out destroy the enzyme treatment between 9 ~ 10.
5）Remaining fibroin fiber is filtered out, with the bag filter of molecular cut off 3500 to silk fibroin protein solution dialysis 48h, Obtain pure silk fibroin protein solution.
6）Silk fibroin protein solution is freeze-dried 48h, silk fibroin powder is obtained.
Although protease is with higher specificity, single enzymatic isolation method only has 10% or so, dissolving to the solubility of fibroin albumen Efficiency is very low, and longer to the processing time of silk.So the present invention is first at relatively high temperatures using ionic liquid to fibroin Fiber is tentatively swelled and dissolved, and adds alkali protease and fibroin albumen is dissolved, and is shortening the same of action time When, the solubility to fibroin albumen can also be improved, its solubility is up to more than 20%.
Raw materials used in the present invention, equipment, is the conventional raw material, equipment of this area unless otherwise noted；In the present invention Method therefor, is the conventional method of this area unless otherwise noted.
It is described above, only it is presently preferred embodiments of the present invention, not the present invention is imposed any restrictions, it is every according to the present invention Any simple modification, change and equivalent transformation that technical spirit is made to above example, still fall within the technology of the present invention side The protection domain of case.

Claims (7)

1. a kind of method that utilization ionic liquid and protease extract tussah silk fibroin albumen, it is characterised in that in terms of g and mL, Comprise the following steps：
1）3.5-4.5g tussah silks are weighed, are cleaned with deionized water, surface contaminant, drying is removed；
2）By the tussah silk of drying with 1:95-105 bath raio is in the Na containing 0.4-0.6wt%2PO4With 0.8-1.2wt%'s C17H3525-35min is boiled in COONa mixed solution, degumming process is carried out, altogether degumming four times；
3）After degumming, tussah silk is washed by rubbing with the hands more than 4 times with deionized water, 55-65 DEG C of baking oven is put into, obtain dry fibroin fine Dimension；
4）The fibroin fiber of 1.8-2.2g dryings is weighed, with 1:In 45-55 bath raio immersion [AMIM] Cl ionic liquids, control is anti- Answer temperature in the range of 80 ~ 100 DEG C, 5-7h is stirred in oil bath, after the completion of reaction, be cooled to room temperature；
5）To step 4）PM is added in intermediate ion liquid13- basic protein enzyme powder 0.25-0.35g, in 40 ~ 55 DEG C of oil bath 5-7h is stirred, is obtained after fibroin/ionic liquid solution, the enzyme that goes out is carried out；
6）Treat that fibroin/ionic liquid solution is cooled to room temperature, add absolute ethyl alcohol, soak repeatedly, separate out fibroin albumen, to mixed Compound is filtered by vacuum, and deionized water is added into the fibroin albumen filtered out, is filtered after soaking repeatedly, then solution is loaded into saturating Dialyse 20-28h in analysis bag, obtains pure silk fibroin protein solution；
7）Obtained silk fibroin protein solution is freeze-dried, you can obtain silk fibroin powder.
2. the method that a kind of utilization ionic liquid and protease as claimed in claim 1 extract tussah silk fibroin albumen, it is special Levy and be, step 4）In, the preparation method of [AMIM] the Cl ionic liquids is：By 1.0-1.5:1 mol ratio is held to reaction Allyl chloride and N- methylimidazoles are added in device, 6 ~ 8h is stirred at reflux in 55-65 DEG C of oil bath, after completion of the reaction, using rotation Evaporation removes excessive 2- methallyl chlorides, obtains light yellow clear liquid, i.e. [AMIM] Cl ionic liquids, is freeze-dried After 20-28h, thaw stand-by.
3. the method that a kind of utilization ionic liquid and protease as claimed in claim 1 extract tussah silk fibroin albumen, it is special Levy and be, step 5）In, the PM13The preparation method of-alkali protease is：Sodium borate buffer to 90-110mL 0.1M is molten 0.10-0.20g alkali proteases and 3.5-4.0g PM are added in liquid13, 0.5-1.5h is slowly stirred under the conditions of 1-5 DEG C, outside Plus ultrafiltration removes unreacted PM under conditions of 45-55mL 1mmol/L HCl13, in triplicate, filtrate is freeze-dried, Obtain PM13- basic protein enzyme powder, it is stand-by.
4. the method that a kind of utilization ionic liquid and protease as claimed in claim 3 extract tussah silk fibroin albumen, it is special Levy and be, the PM13For MW15000 pectinations PEG.
5. the method that a kind of utilization ionic liquid and protease as claimed in claim 4 extract tussah silk fibroin albumen, it is special Levy and be, in PM13In the preparation process of-alkali protease, before freeze-drying, 4h is freezed under the conditions of -20 DEG C in advance.
6. the method that a kind of utilization ionic liquid and protease as claimed in claim 1 extract tussah silk fibroin albumen, it is special Levy and be, step 5）In, go out enzyme when, at least 30min is incubated in the oil bath more than 80 DEG C.
7. the method that a kind of utilization ionic liquid and protease as claimed in claim 1 extract tussah silk fibroin albumen, it is special Levy and be, step 6）In, the molecular cut off of the bag filter is 3500.
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CN110759969A (en) * 2019-10-14 2020-02-07 浙江海洋大学 Preparation method of antioxidant enzymolysis oligopeptide from peripherical glands of northern pacific squid
CN113603903A (en) * 2021-08-04 2021-11-05 上海曜爱生物科技有限公司 Preparation and application of fillable silk fibroin gel

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Cited By (6)

* Cited by examiner, † Cited by third party
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CN109295140A (en) * 2018-10-22 2019-02-01 浙江海洋大学 A kind of preparation method of Japanese croaker air bladder collagen protein source dipeptidyl peptidase-IV peptide for inhibiting
CN109295140B (en) * 2018-10-22 2022-01-18 浙江海洋大学 Preparation method of collagen-derived dipeptidyl peptidase-IV (DPP-IV) inhibitory peptide of Nibea japonica swim bladder
CN110759969A (en) * 2019-10-14 2020-02-07 浙江海洋大学 Preparation method of antioxidant enzymolysis oligopeptide from peripherical glands of northern pacific squid
CN110759969B (en) * 2019-10-14 2021-10-22 浙江海洋大学 Preparation method of antioxidant enzymolysis oligopeptide from peripherical glands of northern pacific squid
CN113603903A (en) * 2021-08-04 2021-11-05 上海曜爱生物科技有限公司 Preparation and application of fillable silk fibroin gel
CN113603903B (en) * 2021-08-04 2023-10-10 上海曜爱生物科技有限公司 Preparation and application of silk fibroin gel capable of being filled

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Application publication date: 20171020