CN107266494A - Method of phosphatide and products thereof is extracted in a kind of fish head from grass carp - Google Patents
Method of phosphatide and products thereof is extracted in a kind of fish head from grass carp Download PDFInfo
- Publication number
- CN107266494A CN107266494A CN201710543856.7A CN201710543856A CN107266494A CN 107266494 A CN107266494 A CN 107266494A CN 201710543856 A CN201710543856 A CN 201710543856A CN 107266494 A CN107266494 A CN 107266494A
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- CN
- China
- Prior art keywords
- fish head
- grass carp
- phosphatide
- ethanol
- extracted
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 51
- 241000252230 Ctenopharyngodon idella Species 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 50
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000012634 fragment Substances 0.000 claims abstract description 18
- 238000000605 extraction Methods 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 15
- 239000000469 ethanolic extract Substances 0.000 claims abstract description 14
- 239000002002 slurry Substances 0.000 claims abstract description 11
- 238000005119 centrifugation Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000006835 compression Effects 0.000 claims description 4
- 238000007906 compression Methods 0.000 claims description 4
- 238000002390 rotary evaporation Methods 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 3
- 239000010409 thin film Substances 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 238000010257 thawing Methods 0.000 claims description 2
- 238000003828 vacuum filtration Methods 0.000 claims description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- 238000011010 flushing procedure Methods 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- 239000000284 extract Substances 0.000 abstract description 3
- 238000004886 process control Methods 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 11
- 150000003904 phospholipids Chemical class 0.000 description 4
- 239000013505 freshwater Substances 0.000 description 3
- 241000252210 Cyprinidae Species 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- 241001275898 Mylopharyngodon piceus Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 201000007094 prostatitis Diseases 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/10—Phosphatides, e.g. lecithin
- C07F9/103—Extraction or purification by physical or chemical treatment of natural phosphatides; Preparation of compositions containing phosphatides of unknown structure
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention discloses a kind of method that phosphatide is extracted in fish head from grass carp and products thereof, this method comprises the following steps：1) clean grass carp fish head is minced into the fish head fragment of 1~2cm diameters.2) fish head fragment and 95% ethanol are according to mass volume ratio 1:10~1:20 mixing, obtain pending thing.3) pending thing is carried out to the high-speed breakage of fish head fragment in homogenate extraction device, slurries are obtained.4) slurries are filtered, obtains ethanol extract.5) ethanol extract removing ethanol is obtained into residue, the residue is stirred with acetone and mixed, flaxen translucent thick acetone insoluble matter is obtained after centrifugation, grass carp fish head phosphatide finished product, purity >=85% is produced.The present invention only using being easy to get very much and the extract solution harmless to extract component in extraction process, and coordinates efficient process control, you can realize the high efficiency extraction of phosphatide in grass carp fish head by extremely easy technical process.
Description
Technical field
The invention belongs to extract the technical field of phosphatide with large cultivate fresh water fishes for raw material, specifically, it is related to one kind
Method of phosphatide and products thereof is extracted from grass carp fish head.
Background technology
Grass carp (Ctenopharyngodon idellus) category Cypriniformes Cyprinidae Leuciscinae grass carp category, has been commonly called as：Grass carp,
Grass carp, oily grass carp, careless grass carp, ctenopharyngodon idellus, charlatan, black black carp etc..Grass carp is one of four large Chinese carps of CHINESE FRESHWATER cultivation, and meat is preferable,
Flavour is delicious, is loved by consumers, and because its growth is rapid, feed resource is wide, and deep to be favored by culturist, cultivation amount is at home
In fresh-water fishes prostatitis.
Grass carp turns into domestic important aquatic products processing kind in recent years, and its fish head weight accounts for the 20~30% of full fish, but food
Not high with being worth, work in-process lacks effective Application way as main accessory substance.Grass carp head is rich in lecithin and insatiable hunger
And aliphatic acid, with higher active lipids value of exploiting and utilizing, also lack both at home and abroad at present and phosphatide in grass carp fish head is carried out
Effectively extract the technology of processing.
The content of the invention
It is an object of the invention to provide a kind of safety and environmental protection, easy to operate and be easily achieved large-scale industrial production
Method of phosphatide and products thereof is extracted from grass carp fish head.
Its concrete technical scheme is：
Method of phosphatide and products thereof is extracted in a kind of fish head from grass carp, is comprised the following steps：
1) clean grass carp fish head is minced into the fish head fragment of 1~2cm diameters.
2) by step 1) obtained fish head fragment and 95% ethanol is according to mass volume ratio 1:10~1:20 mixing, are obtained
Pending thing.
3) by step 2) obtained pending thing carries out the high-speed breakage of fish head fragment in homogenate extraction device, starched
Liquid.
4) by step 3) obtained slurries are filtered, and obtain ethanol extract.
Specifically, filter type is vacuum filtration or plate compression.
5) by step 4) obtained ethanol extract removes ethanol and obtains residue, by the residue and acetone according to matter
Measure volume ratio 1:5~1:10 are stirred after mixing, centrifugation and obtain flaxen translucent thick acetone insoluble matter, produce
Grass carp fish head phosphatide finished product.
Wherein, specifically：
Step 1) described in grass carp fish head be the fish head of fresh the removings fish gill, the fish head or solution that freeze after the removing fish gill
The fish head of the fish gill is removed after jelly；
Step 1) described in the cleaning way of grass carp fish head be：Fish head is gone the clear water after the cheek below 10 DEG C rinse,
Wash away blood stains etc., then drainage.
It is preferred that, step 3) in homogenate extraction operating temperature be 20~25 DEG C.
Specifically：Step 5) in the removing mode of ethanol evaporated for rotary evaporation in vacuo or thin film evaporator；
Step 5) in centrifugation be horizontal centrifuge centrifuge or disc centrifuge centrifugation；
It is preferred that, step 5) in stirring mixing temperature conditionss be 20~25 DEG C；
Further, step 5) in ethanol extract remove the obtained ethanol of ethanol process it is recyclable and with new addition
The mixing of 95% ethanol after carry out step 2), the amount ratio of obtained recoverys ethanol and 95% ethanol newly added is 1:1~3:
1。
Present invention also offers the grass carp fish head phosphatide obtained according to the method that phosphatide is extracted in the above-mentioned fish head from grass carp
Finished product, mass fraction >=85% of phosphatide.
Compared with prior art, beneficial effects of the present invention：
The present invention is by extremely easy technical process, only using being easy to get very much and harmless to extract component in extraction process
Extract solution, and coordinate efficient process control, you can realize the high efficiency extraction of phosphatide in grass carp fish head.And the present invention's is final
Product is after measured >=85% containing phospholipid purity.The product has extensively in fields such as biotechnology, health care and food industry
General application, with important scientific value and economic benefit.During the ethanol that uses can reclaim after reuse, it is economical
Environmental protection.
Embodiment
Technical scheme is described in more detail with reference to specific embodiment.
Embodiment 1
The fish head of the fresh removing fish gill is rinsed well with less than 10 DEG C of clear water, then drainage, fish head is minced into 1cm
The fish head fragment of diameter.Fish head fragment and 95% ethanol are according to mass volume ratio 1:10 mixing, obtain pending thing.
The high-speed breakage of fish head fragment is carried out in homogenate extraction device, slurries are obtained.The operating temperature of homogenate extraction is 20 DEG C.Obtain
Slurries are filtered, and obtain ethanol extract.Obtained ethanol extract rotary evaporation in vacuo removing ethanol is filtered by vacuum to obtain
Residue, by the residue and acetone according to mass volume ratio 1:Mixing, horizontal centrifuge centrifugation are stirred at 5,20 DEG C
Flaxen translucent thick acetone insoluble matter is obtained afterwards, grass carp fish head phosphatide finished product is produced, and its phospholipid purity is 89%.
Embodiment 2
The fish head freezed after the fish gill is removed to be rinsed with less than 10 DEG C clear water, then drainage, fish head is minced into 2cm diameters
Fish head fragment.Fish head fragment and 95% ethanol are according to mass volume ratio 1:15 mixing, obtain pending thing.In homogenate extraction
The high-speed breakage of fish head fragment is carried out in device, slurries are obtained.The operating temperature of homogenate extraction is 23 DEG C.Obtained slurries are carried out
Filtering, obtains ethanol extract.The ethanol extract thin film evaporator evaporating ethanol that plate compression is obtained obtains residue,
By the residue and acetone according to mass volume ratio 1:Mixing is stirred at 7,23 DEG C, is obtained after disc centrifuge centrifugation
Flaxen translucent thick acetone insoluble matter, produces grass carp fish head phosphatide finished product, and its phospholipid purity is 87%.
Embodiment 3
The fish head for removing the fish gill after defrosting is rinsed with less than 10 DEG C clear water, then drainage, and fish head is minced into 2cm diameters
Fish head fragment.Fish head fragment and 95% ethanol are according to mass volume ratio 1:20 mixing, obtain pending thing.In homogenate extraction
The high-speed breakage of fish head fragment is carried out in device, slurries are obtained.The operating temperature of homogenate extraction is 25 DEG C.Obtained slurries are carried out
Filtering, obtains ethanol extract.The ethanol extract rotary evaporation in vacuo that plate compression is obtained removes ethanol and obtains residue, will
The residue is with acetone according to mass volume ratio 1:Mixing is stirred at 10,25 DEG C, obtains light after horizontal centrifuge centrifugation
The translucent thick acetone insoluble matter of yellow, produces grass carp fish head phosphatide finished product, and its phospholipid purity is 86%.
The foregoing is only a preferred embodiment of the present invention, protection scope of the present invention not limited to this, any ripe
Those skilled in the art are known in the technical scope of present disclosure, the letter for the technical scheme that can be become apparent to
Altered or equivalence replacement are each fallen within protection scope of the present invention.
Claims (10)
1. the method for phosphatide is extracted in a kind of fish head from grass carp and products thereof, it is characterised in that comprise the following steps：
1) clean grass carp fish head is minced into the fish head fragment of 1~2cm diameters；
2) by step 1) obtained fish head fragment and 95% ethanol is according to mass volume ratio 1:10~1:20 mixing, obtain waiting to locate
Manage thing；
3) by step 2) obtained pending thing carries out the high-speed breakage of fish head fragment in homogenate extraction device, obtains slurries；
4) by step 3) obtained slurries are filtered, and obtain ethanol extract；
5) by step 4) obtained ethanol extract removes ethanol and obtains residue, by the residue and acetone according to mass body
Product compares 1:5~1:10 are stirred after mixing, centrifugation and obtain flaxen translucent thick acetone insoluble matter, produce grass carp
Fish head phosphatide finished product.
2. the method for phosphatide is extracted in the fish head according to claim 1 from grass carp and products thereof, it is characterised in that
Step 1) described in grass carp fish head be the fish head of fresh the removings fish gill, after the removing fish gill after the fish head that freezes or defrosting
Remove the fish head of the fish gill.
3. the method for phosphatide is extracted in the fish head according to claim 1 from grass carp and products thereof, it is characterised in that step 1)
Described in the cleaning way of grass carp fish head be：Fish head is gone after the cheek with flushing in less than 10 DEG C of clear water, blood stains are washed away, then filter
Water.
4. the method for phosphatide is extracted in the fish head according to claim 1 from grass carp and products thereof, it is characterised in that step 3)
The operating temperature of middle homogenate extraction is 20~25 DEG C.
5. according to method that phosphatide is extracted from grass carp fish head described in claim 1 and products thereof, it is characterised in that step 4) in
Filter type for vacuum filtration or plate compression.
6. the method for phosphatide is extracted in the fish head according to claim 1 from grass carp and products thereof, it is characterised in that step 5)
The removing mode of middle ethanol is that rotary evaporation in vacuo or thin film evaporator evaporate.
7. the method for phosphatide is extracted in the fish head according to claim 1 from grass carp and products thereof, it is characterised in that step 5)
Middle centrifugation is that horizontal centrifuge is centrifuged or disc centrifuge centrifugation.
8. the method for phosphatide is extracted in the fish head according to claim 1 from grass carp and products thereof, it is characterised in that step 5)
The temperature conditionss of middle stirring mixing are 20~25 DEG C.
9. the method for phosphatide is extracted in the fish head according to claim 1 from grass carp and products thereof, it is characterised in that step 5)
The ethanol recovery and 95% ethanol with newly adding that middle ethanol extract removing ethanol process is obtained carry out step after mixing
2) amount ratio of the recovery ethanol, obtained and 95% ethanol newly added is 1:1~3:1.
10. the grass carp fish head phosphorus that a kind of method that phosphatide is extracted from grass carp fish head described in any one of claim 1-9 is obtained
Fat finished product, it is characterised in that mass fraction >=85% of the phosphatide in described grass carp fish head phosphatide finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710543856.7A CN107266494A (en) | 2017-07-05 | 2017-07-05 | Method of phosphatide and products thereof is extracted in a kind of fish head from grass carp |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710543856.7A CN107266494A (en) | 2017-07-05 | 2017-07-05 | Method of phosphatide and products thereof is extracted in a kind of fish head from grass carp |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107266494A true CN107266494A (en) | 2017-10-20 |
Family
ID=60073174
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710543856.7A Pending CN107266494A (en) | 2017-07-05 | 2017-07-05 | Method of phosphatide and products thereof is extracted in a kind of fish head from grass carp |
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| Country | Link |
|---|---|
| CN (1) | CN107266494A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110862871A (en) * | 2019-11-13 | 2020-03-06 | 武汉轻工大学 | Method for efficiently enriching n-3PUFA lipid from aquatic product processing by-products |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103709193A (en) * | 2013-12-30 | 2014-04-09 | 江苏省农业科学院 | Method for extracting phospholipid from animal livers |
| CN105601666A (en) * | 2016-01-12 | 2016-05-25 | 武汉轻工大学 | Method for extracting phospholipid from heads of hypophthalmichthys molitrix and product of method |
| CN106588977A (en) * | 2016-12-13 | 2017-04-26 | 山东省科学院生物研究所 | Method for extracting high-purity marine phospholipids from Euphausia superba |
-
2017
- 2017-07-05 CN CN201710543856.7A patent/CN107266494A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103709193A (en) * | 2013-12-30 | 2014-04-09 | 江苏省农业科学院 | Method for extracting phospholipid from animal livers |
| CN105601666A (en) * | 2016-01-12 | 2016-05-25 | 武汉轻工大学 | Method for extracting phospholipid from heads of hypophthalmichthys molitrix and product of method |
| CN106588977A (en) * | 2016-12-13 | 2017-04-26 | 山东省科学院生物研究所 | Method for extracting high-purity marine phospholipids from Euphausia superba |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110862871A (en) * | 2019-11-13 | 2020-03-06 | 武汉轻工大学 | Method for efficiently enriching n-3PUFA lipid from aquatic product processing by-products |
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| PB01 | Publication | ||
| PB01 | Publication | ||
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Application publication date: 20171020 |