CN107258690B - Detection device and method for detecting influence of hypoxia on fruit flies - Google Patents
Detection device and method for detecting influence of hypoxia on fruit flies Download PDFInfo
- Publication number
- CN107258690B CN107258690B CN201710363287.8A CN201710363287A CN107258690B CN 107258690 B CN107258690 B CN 107258690B CN 201710363287 A CN201710363287 A CN 201710363287A CN 107258690 B CN107258690 B CN 107258690B
- Authority
- CN
- China
- Prior art keywords
- hypoxia
- hours
- inverted
- anoxic chamber
- coiled pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses an efficient and reliable determination method, which is used for determining the influence of oxygen deficiency on fruit fly behaviors. Also discloses a precise device for researching the influence of hypoxia on fruit flies and application of the device in screening hypoxia-related genes. Wherein, in the method, the time for giving the fruit fly hypoxia is preferably 2.5-3 hours, and the climbing ability measurement is carried out 4-6 hours after the hypoxia treatment.
Description
Technical Field
The present invention relates generally to the field of life sciences, and more particularly to a detection apparatus and method for detecting the effect of hypoxia on fruit flies. The invention also discloses the application of the device.
Fund support
The Chinese national key base research program (2013 CB 530900) and the national Natural science Foundation (81371400; 81571101; and 81071026).
Background
Oxygen is essential to life support, while hypoxia can have adverse consequences for the body and tissues. Due to its high energy demand, the brain is more susceptible to hypoxia than other organs. When cerebral blood flow is reduced, the brain may stroke, resulting in the death of cells and tissues (Moskowitz, M. A., Lo, E.H. & Iadecola, C. Heart disease and stroke statistics-2011 update: a report from the American Heart Association. Neuron 67, 181-. In people over The age of 60, stroke is The second leading cause of death (Feigin, V.L. et al. Global and regional bulb of stroke duration 1990): terms from The Global bulb of Disease Study 2010. The Lancet 383, 245-. It is expected that one will die every three to four minutes from a stroke, or suffer severe disability or other neurological disorders from a stroke. This results in a huge economic burden of care and treatment for stroke patients worldwide, and thus, stroke prevention and treatment is not only a medical problem but also a social problem (Johnston, s. c., Mendis, s. & coats, c.d. Global variation in stroke garden and sport: animals from monitoring, surveillance, and modelling, The lancet. Neurology 8, 345-354 (2009)).
Hypoxic injury can result from ischemia causing a reduction in the blood supply to hypoxic and nutritionally deficient tissues. Ischemia can cause severe tissue damage by activating cell death. However, post-ischemic reperfusion (restoration of blood supply) results in more damage in so-called ischemia/reperfusion injury. Prolonged repeated reperfusion after hypoxia may impair mitochondrial activity and respiratory chain, may cause increased oxidative stress and subsequent injury or inflammation (Lighton, J.R. & Schilman, P.E. Oxygen reperfusion in an injury. PloS one 2, e1267 (2007); Idris, A.H. et al. oxidative in therapy systems lateral after cardiac injury, cardiac reperfusion injury, and reperfusion. Critical care media 33, 2043-, 1683-1698 (2013).).
Several hypotheses about ischemia-induced injury have been proposed over the past decades, the main being that energy deficiency leads to massive release of glutamate, disruption of ionic balance and oxidative stress (Moskowitz, m. a., Lo, e.h. & iaddecola, c. Heart disease and stroke statistics-2011 update: a report from the American Heart association. Neuron 67, 181-. Over 1000 potential Stroke drugs or interventions have been developed for the processes or molecules involved in these hypotheses, some of which have been subjected to approximately 160 clinical trials, but none have been successful in the clinic (Ginsberg, m.d. Current status of neural protection for nuclear biochemistry: synthetic overview Stroke 40, S111-114 (2009)). One way to alter this condition is by genetic or chemical screening to discover new molecules or signaling pathways that modulate the organism's susceptibility to hypoxic injury or ability to tolerate hypoxia.
The fruit flies have short life span and can breed a large number of offspring under the condition of consuming low resources. The drosophila genome contains approximately 14,000 genes, many of which are conserved in mammals, and about 75% of the genes of known human diseases find homologous genes in drosophila. In addition, the Drosophila research community has accumulated various mutants, transgenic lines, and gene editing tools/techniques. Thus, Drosophila is often used to model human diseases, particularly neurological diseases (Bilen, J. & Bonnii, N.M. Drosophila as a model for human neurointegrative disease, Annual review of genetics 39, 153-171 (2005); Lloyd, T.E. & Taylor, J.P. Flightless flies: Drosophila models of neurousaular disease, Ann N Y Acad Sci 1184, e1-20 (2010)), which can be further used in genetic and chemical screens to identify new molecules that mediate/modulate hypoxia-induced damage and potential drugs.
Compared to mammals, drosophila have a very strong ability to tolerate prolonged hypoxia. Drosophila melanogaster subjected to long-term hypoxia can recover and reactivate when placed in air at room temperature (Hermes-Lima, M. & Zenteno-Savin, T. Animal stress to dry change in oxidative activity and physiological oxidative stress, Comparative biological chemistry and physiological stress, oxidative & pharmacological: CBP 133, 537 556 (2002)). However, hypoxia/reoxidation (A/R) stress causes damage in fruit flies because a proportion of the flies die in the following days after several hours of hypoxia (Vigne, P., Tauc, M. & Frelin, C. Strong dialect restrictions technique Drosophila against oxidation and reoxidation in diodes, PloS one 4, e5422 (2009); Carabato, J.C. et al, Role of PON in oxidation and regeneration in diodes, PloS one 9, e84434 (2014)). This, together with the advantages mentioned above, makes Drosophila helpful in studying the molecular mechanisms of susceptibility and tolerance to hypoxia in vivo, and identifying potential targeting drugs and drugs for treating oxygen deficiency induced injury by genetic and chemical screening (Bartholomew, N. R., Burdet, J. M., Vande Brooks, J. M., Quinlan, M.C. & Call, G.B. Impaired binding and flight behaviour in drainage and tumor formation carbon dioxide and Scientific delivery 5, 15298 (2015); Haddad, G.G. Tolerand low O2: stress from expression production models, expression physical chemistry 91, 277 (2006); Zhou, D.D., Visk., D.W. G.G. gravity, expression of protein, 277 (247) and coding sequence of expression of peptide of protein 239 (247). To assist in these studies, we developed a sophisticated means of completely depriving flies of oxygen, and a very reliable assay for quantitatively examining the mortality and recovery of motor capacity of flies that have experienced hypoxia.
Disclosure of Invention
In one aspect of the present invention, there is provided an anoxic apparatus, characterized in that the anoxic apparatus comprises the following devices:
a. a gas flow indicator;
b. a water tank;
c. an anoxic chamber;
wherein the devices are connected by adopting a pipeline, and gas sequentially passes through the devices; an inverted plastic tube is arranged in the anoxic chamber.
In one embodiment of the present invention, the anoxic chamber is a bilayer platform comprising: a mesh net, a plastic plate with holes and an inverted funnel; the inverted funnel is positioned in the center of the double-layer platform, the large opening of the inverted funnel is positioned below the mesh net, and the small opening of the inverted funnel is connected into the gas pipeline of the oxygen-poor device through a coiled pipe.
In a specific embodiment of the invention, the diameter of the serpentine is 1.0 cm or less.
In one embodiment of the invention, the anoxic chamber further comprises a cover with a central hole, the coiled pipe connected with the small opening of the inverted funnel passes through the central hole, and the diameter of the central hole is larger than that of the coiled pipe connected with the small opening of the inverted funnel.
In one embodiment of the invention, the outer layer of the serpentine between the anoxic chamber cover and the water tank is sleeved with a larger diameter serpentine and tightly connected with the central hole on the anoxic chamber cover.
In a particular embodiment of the invention, the larger diameter serpentine tube has a diameter of 2.0 centimeters or more.
In one embodiment of the invention, the inverted plastic tube is an EP tube, preferably a 1.5 mL EP tube.
In a specific embodiment of the invention, the opening of the inverted plastic tube is covered with gauze.
In one embodiment of the invention, the gas is selected from nitrogen.
In the present invention, the device can be used to detect the effect of hypoxia on fruit flies.
In the present invention, the device can be used to screen for hypoxia injury related genes or to reveal the mechanism of tissue damage in hypoxia related diseases.
In one embodiment of the invention, the hypoxia-related disease comprises hypertension, diabetes, heart disease, stroke, cancer, preferably ischemic stroke.
In another aspect of the invention, a method of detecting the effect of hypoxia on fruit flies is provided, characterized in that a device according to any one of claims 1 to 9 is used.
In one embodiment of the invention, the drosophila is given hypoxia for a period of preferably 2.5-3 hours, and the climbing ability assay is performed 4-6 hours after the hypoxia treatment.
Drawings
Fig. 1 shows a schematic and an actual view of an oxygen depletion device. (A) Schematic (top) and actual image (bottom) of the hypoxic device. The nitrogen flow direction is marked by green arrows. Additional gas from the sealed anoxic chamber is vented through the black pipe into the open water tank (red arrows and dots). (Bi) Drosophila was placed in a 1.5 mL EP tube, the lid was removed and replaced with gauze. The (Bii) EP tube was inverted in the well of the 2-layer platform.
FIG. 2 shows the effect of length of hypoxic period on A/R-induced mortality. (A) Graph of the cumulative mortality percentage of drosophila subjected to different hypoxic periods on the following days-to the fifth. For each data point, n = 5. (B) graph and straight line fit of cumulative mortality percentage 5 days after hypoxia versus hypoxic period (hours, h).Line showing best-fit equation, R2AndPthe value is obtained. Data are presented as mean ± SEM, statistical analysis using one-way anova, Tukey multiple comparison t-test,. p<0.05. Flies were evaluated 5 days after emergence.
Figure 3 shows the use of a climbing capacity assay to detect the recovery of fly motility in the consequence of hypoxia/reoxidation. (A) Anoxic consequences the locomotor ability of flies was evaluated as a function of recovery time (reoxidation time) by analyzing the height of climb (cm) of each fly. (B) To further expand the study, the locomotor capacity of the flies was assessed by climbing height (cm) after another 5 hours of oxidation after different periods of hypoxia (h). Data are presented as mean ± SEM, statistical analysis using one-way anova, Tukey multiple comparison t-test, × p < 0.05. Flies were evaluated 5 days after emergence, with n = 20 flies per group.
Detailed Description
The present invention is described in further detail below with reference to specific embodiments, which are given for the purpose of illustration only and are not intended to limit the scope of the invention. The experimental procedures in the following examples are conventional unless otherwise specified. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
In the present invention, this detection method is superior to other conventional hypoxic screening devices because it is easy to use, suitable for large-scale studies and produces reproducible results. The hypoxic device can be used quickly, efficiently, and at low cost. The device will help provide new insights into the mechanisms of tissue damage after exposure to an impaired oxygen supply (e.g. ischemic stroke).
As used herein, "ischemic stroke" refers to ischemic stroke, which is a generic term for necrosis of brain tissue due to stenosis or occlusion of blood supply arteries (carotid and vertebral) of the brain, insufficient blood supply to the brain. There are four types of cerebral ischemia: transient Ischemic Attack (TIA); reversible neurological dysfunction (RIND); progressive Stroke (SIE); complete Stroke (CS). TIA did not have cerebral infarction, while RIND, SIE and CS had different degrees of cerebral infarction.
Examples
The invention is further illustrated by the following examples. The examples are provided for illustrative purposes only and should not be construed as limiting the scope or content of the invention in any way.
Example 1: drosophila family and culture
The gene Wild Type (WT) w1118 of Drosophila melanogaster was maintained in standard corn flour-agar-brown sugar-yeast medium at a relative humidity of 50-70% and cultured at 25 ℃ for 12/12 hours in a diurnal cycle. The formula of the culture medium is as follows: ddH20.65L/L of O, 16 g/L of baker's yeast, 80 g/L of corn flour, 6.5 g/L of agar, 137.5 g/L of brown sugar, 7.5 g/L of beer yeast, 2 g/L of methyl p-hydroxybenzoate, 20 mL/L of ethanol, ddH2O100 mL/L and propionic acid 6.25 mL/L, prevent the growth of mould. Male and female fruit flies were identified under a dissecting microscope. At the time of the study, adult drosophila were selected between 4-6 days post-pupation.
Example 2: oxygen-deficient device
The design and actual image of the oxygen-deficient device are shown in fig. 1, and the oxygen-deficient device consists of an oxygen-deficient chamber (a cylindrical transparent closed container with the diameter of 12 cm and the height of 14 cm), a water tank, a gas flow indicator and a pipeline. The oxygen-deficient chamber has a double-layer circular platform, the bottom layer is a net, and the upper layer is a plastic plate with holes. In each well, a 1.5 mL EP tube can sit and hold 10-20 fruit flies. The lid of the EP tube was removed, covered with gauze and inverted on the platform. A plastic funnel was mounted in the center of the platform, the tip of the funnel was connected to a small serpentine (1.0 cm diameter), pure nitrogen (N)2) Through the funnel to the central bottom of the anoxic chamber, oxygen (O)2Than N2Heavy) flows in the EP tube and can pass through another larger serpentine (2.0 cm in diameter) in the anoxic chamber, exiting completely through a hole in the center of the lid, which is tightly attached to that hole. N is a radical of2The conveying coils are placed in larger pipes, both of which enter the tank, thus allowing the gas from the anoxic chamber to escape, but blocking itBlocking air from entering the room. Carrying N2Is led out of the water tank, is connected with a gas flow indicator and is further connected with a compressed pure N2N of gas2In the gas cylinder. The silicone and paraffin films are used to prevent gas leakage at the joint. The air tightness at each connection was tested underwater.
Example 3: hypoxia assay
5-day-old fruit flies were rapidly collected under carbon dioxide (1-2 minutes) and recovered in vials 18-24 hours prior to the hypoxic treatment with standard media to minimize confounding effects of carbon dioxide anesthesia. Each vial contained the same number of males and females (10 or 20 total). After recovery, all flies in one vial were carefully transferred to a 1.5 mL EP tube with a funnel and the EP tube was quickly covered with gauze and rubber band to avoid escape. The EP tube containing the spray was then transferred to a platform in an anoxic chamber and pure N was added2Introduced into the anoxic chamber at a constant flow rate of 5L/min. The time is recorded. The drosophila in each vial experienced only one to hypoxic period. All assays were kept in the same chamber and exposed to the same levels of light and noise at 25 ℃ and 50-70% humidity.
All data sets are expressed as mean ± SEM. Statistical analysis was performed using one-way anova, and p <0.05 was considered statistically significant. The software GraphPad Prism was used for statistical analysis. All experiments were run at least in triplicate.
Using this system, young animals w are detected by measuring the effect of length of hypoxia on cumulative mortality and recovery of climbing capacity1118A/R induced lesions in Drosophila Wild Type (WT). In N2In the anoxic chamber, which flowed at a rate of 5L per minute, all flies fell from the EP tube wall to the gauze due to anoxic coma within 3 minutes, and thereafter remained still. After a specified hypoxia time, N is terminated2Flowing into the anoxic chamber, taking out the fruit flies from the anoxic chamber, and recovering activity of all the fruit flies after being oxidized after being anoxic in the air at room temperature. The length of the recovery time depends on the length of the hypoxia time. The longer the hypoxia time, the longer the time (number) that needs to be recoveredAs not shown). However, most of the flies did not recover when the hypoxia time was 6 hours or more. In contrast, when the hypoxic time was less than 1 hour, all drosophila recovered and did not die within the following 5 days (data not shown).
Example 4: mortality analysis
After exposure to 1.0, 1.5, 2, 2.5, 3, 4 and 4.5 hours of hypoxia, the flies in each EP tube were transferred back into standard fly food culture vials. Dead flies in each vial were counted daily over the following 5 days and the cumulative percent mortality for each vial was calculated. To avoid the fruit flies to be caught by the medium, the flasks were placed horizontally the first day after hypoxia.
This study quantitatively investigated the effect of 7 hypoxic periods, 1, 1.5, 2, 2.5, 3, 4 and 4.5 hours on the mortality of drosophila. Pupated wild type 5-day-old drosophila melanogaster were divided into 7 groups, each group faced one of 7 hypoxic periods. For each group, 100 wild fruit flies were divided into 5 subgroups, each group having 10 males and 10 females. As shown in FIG. 2A, some of the flies died daily within 5 days after exposure to hypoxia ≧ 1.5 hours, and the cumulative mortality rose with increasing time over the first 3 days, peaking at the fourth day. The longer the hypoxia time, the higher the cumulative mortality. The data points for the time to hypoxia-cumulative mortality on day five were fitted with a straight line and the equation showed that the cumulative mortality was linearly and positively correlated with the time to hypoxia. Coefficient of correlation R20.9893, high significance (p)<0.0001, fig. 2B). Notably, the cumulative mortality on day five was about 50% when the hypoxia time was 2.5-3 hours.
Example 5: determination of climbing pipe capability
After exposure to the appropriate hypoxic period, the flies were transferred back to their culture flasks and allowed to recover for 5 hours or other indicated time under normoxic conditions before being subjected to an Automated RING (ARING) assay as described previously. Briefly, 10 flies of the same group were transferred to a test vial, which was a clear plastic cylinder with an inner diameter of 2.0 cm and a height of 20.0 cm. The test tube is vertically installed on the aRING device, the device automatically shakes up and down for 4 times under the drive of the stepping control motor to shake all fruit flies to the bottom of the test tube, and then the fruit flies climb along the wall of the test tube. This is recorded. The video was transferred to a computer and a snapshot was taken 5 seconds after the start of climb and analyzed using homemade software rflydetect to measure the climb height of each flight for the 5 th second.
The effect of reoxidation time in air after hypoxia on the recovery of fruit fly motility was also investigated using the aRING assay. 50 males and 50 females 5 days old were aliquoted into 10 groups, deoxygenated in an anoxic device for 2.5 hours, then transferred back to culture tubes containing standard media and air and re-oxygenated for recovery. At 3, 4, 5, 6, 7 hours after reoxidation, 2 of 10 groups were randomly measured and subjected to a climbing assay with aRING, and the climbing height of each fruit fly was measured at 5 seconds after the start of climbing. The average climbing height of 20 flies was calculated. As shown in fig. 3A, the longer the reoxidation, the better the mobility recovery, but the climbing height remained stable during the 4 th to 6 th hours after reoxidation.
We further investigated the effect of different hypoxia times at 0, 1.5, 2, 2.5 and 3 hours on the ability to climb the tube at 5 hours after reoxidation in air. 50 males and 50 females 5 days old fruit flies were equally divided into 10 groups, 2 of the 10 groups were subjected to hypoxia for 0, 1.5, 2, 2.5 or 3 hours and then transferred back to culture tubes containing standard medium and air and re-oxygenated for recovery, and after 5 hours, the climbing ability of each fly was examined by using the aRING assay. As shown in fig. 3B, the longer the hypoxia time, the worse the climbing ability, showing that the motor ability recovers more slowly. The results show that hypoxia exposure is negatively dose dependent on motor function (5.19 cm for 1.5 hours, 3.35 cm for 2 hours, 1.54 cm for 2.5 hours, 0.85 cm for 3 hours, 8.24 cm without exposure to hypoxia).
Discussion of the related Art
Hypoxic injury involves a complex and dynamic process that is not fully understood. To further understand the known pathways involved in hypoxic injury, further studies are needed to identify new molecular targets involved in hypoxia and a/R-induced injury. Drosophila is a powerful model for a variety of genetic screens to identify novel genes involved in different phenotypes（Queliconi, B. B., Kowaltowski, A. J. & Nehrke, K. An anoxia-starvation model for ischemia/reperfusion in C. elegans. Journal of visualized experiments : JoVE(2014).). The assay methods and devices of the invention provide an effective and reliable method for screening genes involved in hypoxia-induced damage and for screening pharmaceutical compounds that reduce hypoxia-induced damage.
In the present invention, w is 5 days old after pupation1118After 1 hour of hypoxia treatment, the disease is fatal, and the mortality rate of 5 days after hypoxia is in linear positive correlation with the time length of the hypoxia period, so that about 40-60% of hypoxia time is 2.5-3 hours. Thus, the present invention demonstrates that a duration of 2.5-3.0 hours of hypoxia can be an appropriate length of time to quantify the positive or negative impact of genetic manipulation or chemical application on mortality. In addition, after 2.5 hours of anoxic treatment, the migration recovery is slow, and the climbing height at 4-6 hours is basically kept unchanged. Therefore, we propose treatment with 2.5-3 hours of hypoxia and 4-6 hours of recovery prior to mobility analysis for future hypoxia assays.
Conventional hypoxic devices include plastic tubes containing single or multiple fruit flies for testing, and can monitor oxygen levels and temperatures during hypoxic testing using specially designed chambers (Zhou, d., Visk, d.w. & Haddad, g.g. Drosophila, a gold bug, for the diagnosis of the genetic basis of birth and science to hypoxia. petritric research 66, 239-247, (2009)). Therefore, we developed a simple hypoxia assay to study oxygen deficiency in flight, which is more efficient and can simultaneously quantify the performance of many different genotypes of drosophila. The device may help overcome many of the limitations known in the use of current hypoxia trials and will help to further understand the underlying mechanisms of hypoxic diseases such as hypoxic stroke.
Is incorporated by reference
The entire disclosure of each patent and scientific literature cited herein is incorporated by reference for all purposes.
Equivalence of
The present invention may be embodied in other specific forms without departing from its essential characteristics. Accordingly, the foregoing examples are to be considered as illustrative and not limiting of the invention described herein. The scope of the invention is indicated by the appended claims rather than by the foregoing description, and all changes that come within the meaning and range of equivalency of the claims are intended to be embraced therein.
Claims (7)
1. An hypoxia apparatus for screening hypoxia injury-associated genes or revealing mechanisms of hypoxia-associated disease tissue injury, the hypoxia apparatus comprising:
a. a gas flow indicator;
b. a water tank;
c. an anoxic chamber;
wherein the devices are connected by adopting a pipeline, and gas sequentially passes through the devices; an inverted plastic pipe is arranged in the anoxic chamber; the anoxic chamber is a double-layer platform comprising: a mesh net, a plastic plate with holes and an inverted funnel; the inverted funnel is positioned in the center of the double-layer platform, the large opening of the inverted funnel is positioned below the mesh net, and the small opening of the inverted funnel is connected into a gas pipeline of the oxygen-poor device through a coiled pipe; the diameter of the coiled pipe is less than 1.0 cm; the anoxic chamber also comprises a cover with a central hole, a coiled pipe connected with the small opening of the inverted funnel penetrates through the central hole, and the diameter of the central hole is larger than that of the coiled pipe connected with the small opening of the inverted funnel; the outer layer of the coiled pipe connected between the cover of the anoxic chamber and the water tank is sleeved with a coiled pipe with a larger diameter and is tightly connected with a central hole on the cover of the anoxic chamber; the diameter of the larger diameter serpentine tube is more than 2.0 cm;
the hypoxia-related disease is ischemic stroke.
2. The apparatus of claim 1, wherein the inverted plastic tube is a 1.5 mL EP tube.
3. The apparatus of claim 2 wherein the opening of the inverted plastic tube is covered with gauze.
4. The apparatus of any of claims 1 to 3, wherein the gas is nitrogen.
5. The device of any one of claims 1 to 3, wherein the device is used to detect the effect of hypoxia on fruit flies.
6. A method for detecting the effect of hypoxia on drosophila, using a device according to any of claims 1 to 3.
7. The method of claim 6, wherein the drosophila is administered hypoxia for a period of 2.5-3 hours and the climbing capacity assay is performed 4-6 hours after the hypoxia treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710363287.8A CN107258690B (en) | 2017-05-22 | 2017-05-22 | Detection device and method for detecting influence of hypoxia on fruit flies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710363287.8A CN107258690B (en) | 2017-05-22 | 2017-05-22 | Detection device and method for detecting influence of hypoxia on fruit flies |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107258690A CN107258690A (en) | 2017-10-20 |
| CN107258690B true CN107258690B (en) | 2021-08-10 |
Family
ID=60065138
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710363287.8A Active CN107258690B (en) | 2017-05-22 | 2017-05-22 | Detection device and method for detecting influence of hypoxia on fruit flies |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107258690B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110196991A (en) * | 2019-04-22 | 2019-09-03 | 哈尔滨理工大学 | A kind of motor precommpression holding cavity structure design method based on drosophila algorithm |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1951347B1 (en) * | 2005-11-16 | 2017-01-11 | TreyMed, Inc. | Side-stream respiratory gas monitoring system |
| CN102784013B (en) * | 2012-09-06 | 2015-01-28 | 天津开发区合普工贸有限公司 | Fast shock handling device for small animals through inert gas |
| CN104173119B (en) * | 2014-08-27 | 2016-01-20 | 无锡菩禾生物医药技术有限公司 | A kind of interval hypoxia system |
| CN204890253U (en) * | 2015-07-03 | 2015-12-23 | 杭州艾普仪器设备有限公司 | Continuation ordinary pressure animal oxygen deficiency device |
-
2017
- 2017-05-22 CN CN201710363287.8A patent/CN107258690B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN107258690A (en) | 2017-10-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism | |
| Tai et al. | Tau reduction prevents key features of autism in mouse models | |
| Stewart et al. | Developing zebrafish models of autism spectrum disorder (ASD) | |
| Gröticke et al. | Behavioral alterations in the pilocarpine model of temporal lobe epilepsy in mice | |
| Schneider et al. | Prenatal exposure to valproic acid disturbs the enkephalinergic system functioning, basal hedonic tone, and emotional responses in an animal model of autism | |
| Lari et al. | A novel apparatus for evaluating contaminant effects on feeding activity and heart rate in Daphnia spp. | |
| TW201109658A (en) | Peptoid ligands for isolation and treatment of autoimmune T-cells | |
| Gruss et al. | Distinct activation of monoaminergic pathways in chick brain in relation to auditory imprinting and stressful situations: a microdialysis study | |
| Berlandi et al. | Swing Boat: inducing and recording locomotor activity in a Drosophila melanogaster model of Alzheimer’s disease | |
| CN107258690B (en) | Detection device and method for detecting influence of hypoxia on fruit flies | |
| Totola et al. | Amygdala rapid kindling impairs breathing in response to chemoreflex activation | |
| Boccella et al. | Treatment with 2-pentadecyl-2-oxazoline restores mild traumatic brain injury-induced sensorial and neuropsychiatric dysfunctions | |
| Zhang et al. | Behavioral characterization of CD36 knockout mice with SHIRPA primary screen | |
| Gerhardt et al. | In vivo electrochemical studies of dopamine overflow and clearance in the striatum of normal and MPTP‐treated rhesus monkeys | |
| Fernandez et al. | Differences in locomotor activity across the lifespan of Drosophila melanogaster☆ | |
| Xia et al. | An efficient and reliable assay for investigating the effects of hypoxia/anoxia on Drosophila | |
| Yoshida | Recording the ventilation activity of free-swimming zebrafish and its application to novel tank tests | |
| Woodard et al. | Impaired refinement of kinematic variability in Huntington disease mice on an automated home cage forelimb motor task | |
| Fontana et al. | Modelling ADHD-like phenotypes in Zebrafish | |
| CN109675037B (en) | Application of ATP and receptor thereof in preparation of drugs for treating autism | |
| Blackwell et al. | Spatial Disorientation Under Dark Conditions Across Development in an Alzheimer’s Disease Mouse Model | |
| Tamada et al. | Serotonin disturbance in mouse models of autism spectrum disorders | |
| Erdinger et al. | Lack of APLP1 leads to subtle alterations in neuronal morphology but does not affect learning and memory | |
| Carreño Muñoz | Supervised and unsupervised investigation of the temporal dynamics of normal and pathological behaviour in the mouse and rat | |
| CARRENO-MUÑOZ | DOCTEUR DE L’UNIVERSITÉ DE BORDEAUX |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |