CN107252093A - It is a kind of to be rich in soluble polyphenol and the Guava Leaf and preparation method of flavone aglycone and application - Google Patents
It is a kind of to be rich in soluble polyphenol and the Guava Leaf and preparation method of flavone aglycone and application Download PDFInfo
- Publication number
- CN107252093A CN107252093A CN201710383696.4A CN201710383696A CN107252093A CN 107252093 A CN107252093 A CN 107252093A CN 201710383696 A CN201710383696 A CN 201710383696A CN 107252093 A CN107252093 A CN 107252093A
- Authority
- CN
- China
- Prior art keywords
- guava leaf
- rich
- hemicellulase
- glucosidase
- cellulase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000508269 Psidium Species 0.000 title claims abstract description 102
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 64
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 64
- 229930003944 flavone Natural products 0.000 title claims abstract description 38
- 235000011949 flavones Nutrition 0.000 title claims abstract description 38
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title claims abstract description 36
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 35
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 title claims abstract description 32
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 title claims abstract description 32
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 102000004190 Enzymes Human genes 0.000 claims abstract description 30
- 108090000790 Enzymes Proteins 0.000 claims abstract description 30
- 238000006243 chemical reaction Methods 0.000 claims abstract description 17
- 238000001035 drying Methods 0.000 claims abstract description 14
- 230000000694 effects Effects 0.000 claims abstract description 13
- 238000001976 enzyme digestion Methods 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000007873 sieving Methods 0.000 claims abstract description 8
- 235000013305 food Nutrition 0.000 claims abstract description 6
- 230000036541 health Effects 0.000 claims abstract description 3
- 230000001105 regulatory effect Effects 0.000 claims abstract description 3
- 229940059442 hemicellulase Drugs 0.000 claims description 39
- 108010002430 hemicellulase Proteins 0.000 claims description 39
- 108010059892 Cellulase Proteins 0.000 claims description 36
- 108010047754 beta-Glucosidase Proteins 0.000 claims description 36
- 102000006995 beta-Glucosidase Human genes 0.000 claims description 36
- 229940106157 cellulase Drugs 0.000 claims description 36
- 229940088598 enzyme Drugs 0.000 claims description 29
- 230000002779 inactivation Effects 0.000 claims description 13
- 238000006911 enzymatic reaction Methods 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 241000219991 Lythraceae Species 0.000 claims description 4
- 235000014360 Punica granatum Nutrition 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 3
- 239000004575 stone Substances 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 abstract description 14
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 abstract description 12
- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 abstract description 10
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 abstract description 7
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 abstract description 7
- 235000005875 quercetin Nutrition 0.000 abstract description 7
- 229960001285 quercetin Drugs 0.000 abstract description 7
- UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 abstract description 5
- 235000008777 kaempferol Nutrition 0.000 abstract description 5
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 abstract description 5
- 230000003078 antioxidant effect Effects 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 3
- 239000003963 antioxidant agent Substances 0.000 abstract description 2
- 235000006708 antioxidants Nutrition 0.000 abstract description 2
- 230000005714 functional activity Effects 0.000 abstract description 2
- 241001122767 Theaceae Species 0.000 abstract 1
- 230000003172 anti-dna Effects 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- -1 flavone glycoside Chemical class 0.000 abstract 1
- 229930182470 glycoside Natural products 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 229920003266 Leaf® Polymers 0.000 description 75
- 239000000047 product Substances 0.000 description 18
- 239000000243 solution Substances 0.000 description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 14
- 238000011282 treatment Methods 0.000 description 13
- 239000007788 liquid Substances 0.000 description 10
- 239000011159 matrix material Substances 0.000 description 10
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 9
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 9
- 239000000284 extract Substances 0.000 description 9
- 238000000605 extraction Methods 0.000 description 9
- 230000007062 hydrolysis Effects 0.000 description 9
- 238000006460 hydrolysis reaction Methods 0.000 description 9
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 230000005778 DNA damage Effects 0.000 description 5
- 231100000277 DNA damage Toxicity 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 5
- 230000002292 Radical scavenging effect Effects 0.000 description 5
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 230000007071 enzymatic hydrolysis Effects 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 230000031700 light absorption Effects 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 239000011149 active material Substances 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 235000010980 cellulose Nutrition 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 150000002213 flavones Chemical class 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- 229910002567 K2S2O8 Inorganic materials 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium chloride Substances Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 235000015895 biscuits Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000005961 cardioprotection Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000003810 ethyl acetate extraction Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 229940074391 gallic acid Drugs 0.000 description 1
- 235000004515 gallic acid Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 150000002831 nitrogen free-radicals Chemical class 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000004224 protection Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Substances [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention discloses a kind of rich in soluble polyphenol and the Guava Leaf and preparation method of flavone aglycone and application.The present invention obtains Guava Leaf position by the way that the Guava Leaf cleaned up to be drained to, dried, rubbed broken, sieving；After it is mixed with water again, pH value is regulated, enzyme is added, enzyme digestion reaction is carried out；Terminate the system drying of enzyme digestion reaction, obtain the Guava Leaf product rich in soluble polyphenol and flavone aglycone.The soluble polyphenol content of guava leaf tea obtained by the preparation method is greatly improved, and be the stronger flavone aglycone composition of functional activity by the flavone glycoside ingredient degradation of Guava Leaf, Quercetin, the Aglycones content such as Kaempferol are improved, improves that Guava Leaf is anti-oxidant and anti-DNA damnification effect.So as to which the potentiality that should be applied rich in soluble polyphenol and flavone aglycone Guava Leaf in field of food and/or field of health care products are big.
Description
Technical field
The invention belongs to field of food, more particularly to a kind of Guava Leaf rich in soluble polyphenol and flavone aglycone and
Preparation method and application.
Background technology
Guava Leaf has usage history for many years as a kind of material of medicine-food two-purpose, with anti-oxidant, suppression DNA
Damage, hypoglycemic, anti-inflammatory, antibacterial, hypotensive, a variety of curative effects such as cardioprotection.Many researchs show main in Guava Leaf
Bioactive functions composition includes polyphenols, and these active materials can be eliminated to be drawn by internal excess oxygen or nitrogen free radical
The impaired caused body injury of Antioxidative Defense System risen.Due to plant polyphenol it is main with three kinds of forms (free state,
Conjugation state and binding state) be present in plant, and bind state polyphenol generally with the polysaccharide on plant cell wall, protein with
Chemical bond form is combined, it is difficult to be extracted, causes guava leaf polyphenol class active material utilization low.
Therefore, it is necessary to promote soluble polyphenol and the release of flavone aglycone of Guava Leaf, so as to make full use of a kind stone
Pomegranate leaf Polyphenols active material.
The content of the invention
The primary and foremost purpose of the present invention be to overcome the shortcoming and deficiency of prior art there is provided one kind rich in soluble polyphenol with
And the preparation method of the Guava Leaf of flavone aglycone.
Another object of the present invention is to provide the Guava Leaf product obtained by above-mentioned preparation method.
It is still another object of the present invention to provide the application of described Guava Leaf product.
The purpose of the present invention is achieved through the following technical solutions：A kind of kind stone rich in soluble polyphenol and flavone aglycone
The preparation method of pomegranate leaf, comprises the following steps：
(1) Guava Leaf cleaned up drained, dried, rubbing broken, broken Guava Leaf will be rubbed and sieved, guava is removed
Base of leaf cadre position, obtains the basically identical Guava Leaf position of size；
(2) after the Guava Leaf position for finally giving step (1) is mixed with water, pH value is regulated, enzyme is added, carried out
Enzyme digestion reaction；
(3) step (2) is carried out to the system after enzyme digestion reaction to dry, obtained rich in soluble polyphenol and flavone aglycone
Guava Leaf product.
The condition of drying described in step (1) is preferably in 50~80 DEG C of drying to constant weight；More preferably in 60 DEG C of bakings
Do to constant weight.
The preferential sieve for selecting aperture for 4 mesh of sieving described in step (1).
The consumption of water described in step (2), which is that the Guava Leaf for finally giving step (1) is scattered, to be advisable, to be conducive to
Carry out enzyme digestion reaction；4 times of the Guava Leaf quality preferably finally given equivalent to step (1).
PH value described in step (2) is 4.5~6.0；Preferably 5~5.5.
The temperature of enzyme digestion reaction described in step (2) is 45~55 DEG C；Preferably 50 DEG C.
The time of enzyme digestion reaction described in step (2) is preferably based on 5~8h of each enzyme reaction；It is more preferably each
Plant enzyme reaction 6h meters.
Enzyme described in step (2) be in cellulase, hemicellulase, beta-glucosidase and zytase at least
It is a kind of；Preferably cellulase, hemicellulase and beta-glucosidase is applied in combination.
Described cellulase be preferably enzyme activity be 8000U/g cellulase.
Described hemicellulase be preferably enzyme activity be 8000U/g hemicellulase.
Described beta-glucosidase be preferably enzyme activity be 8000U/g beta-glucosidase.
Described zytase be preferably enzyme activity be 8000U/g zytase.
The quality consumption of described cellulase is preferably equivalent to the 0.5% of Guava Leaf position quality.
The quality consumption of described hemicellulase is preferably equivalent to the 0.5% of Guava Leaf position quality.
The quality consumption of described beta-glucosidase is preferably equivalent to the 0.5% of Guava Leaf position quality.
The quality consumption of described zytase is preferably equivalent to the 0.5% of Guava Leaf position quality.
The detailed process of described enzyme digestion reaction is preferably such as step 1), 2) or 3) shown in, most preferably step 1)：
1) first add cellulase and carry out first time enzymolysis, inactivate cellulase；Add hemicellulase and carry out second
Secondary enzymolysis, inactivates hemicellulase；It is eventually adding beta-glucosidase and carries out third time enzymolysis, inactivates beta-glucosidase；
2) first add hemicellulase and carry out first time enzymolysis, inactivate hemicellulase；Add cellulase and carry out the
Secondary enzymolysis, inactivates cellulase；It is eventually adding beta-glucosidase and carries out third time enzymolysis, inactivates beta-glucosidase；
3) first add beta-glucosidase and carry out first time enzymolysis, inactivate beta-glucosidase；Cellulase is added to enter
Second of enzymolysis of row, inactivates cellulase；It is eventually adding hemicellulase and carries out third time enzymolysis, inactivates hemicellulase.
Step 1), 2) and 3) in,
The reaction condition that described first time enzymolysis, for the second time enzymolysis and third time is digested is respectively preferably in 50 DEG C of reactions
6h；
The condition of described inactivation is preferably 80 DEG C of processing 10min；
The quality consumption of described cellulase is preferably equivalent to the 0.5% of Guava Leaf position quality；
The quality consumption of described hemicellulase is preferably equivalent to the 0.5% of Guava Leaf position quality；
The quality consumption of described beta-glucosidase is preferably equivalent to the 0.5% of Guava Leaf position quality.
The temperature of drying described in step (3) is preferably 50~70 DEG C；More preferably 60 DEG C.
The time preferably at least 12h of drying described in step (3)；More preferably 16h.
A kind of Guava Leaf product rich in soluble polyphenol and flavone aglycone, is obtained by above-mentioned preparation method.
The described Guava Leaf product rich in soluble polyphenol and flavone aglycone is led in field of food and/or health products
Applied in domain；Its is direct-edible；Also varieties of food items can be further processed into, such as rich in soluble polyphenol and flavone aglycone
Guava Leaf tea beverage, the Guava Leaf biscuit rich in soluble polyphenol and flavone aglycone, nutritious food bar etc..
The present invention has the following advantages and effect relative to prior art：
The preparation method that the present invention is provided, is that will be difficult to extract in Guava Leaf by a variety of enzyme hydrolysis, insoluble
The release of state polyphenol is bound, is changed into easy extraction, soluble polyphenol；And the macromolecular functional component of Guava Leaf is degraded to absorption
Ability is stronger, the Aglycones content such as the higher small molecule Quercetin of functional activity and Kaempferol；And the enzyme digestion reaction time is short, condition
Gently, efficiency high, can be used for medicinal plant processing synergy.The preparation method lifts Guava Leaf product oxidation resistance, suppression
DNA damage ability processed, reduces blood glucose, and cholesterol, prevention cardiovascular and cerebrovascular disease etc. is acted on.
Brief description of the drawings
Fig. 1 is the measurement result figure of the not total soluble polyphenol of Guava Leaf and insolubility polyphenol content in be the same as Example.
Fig. 2 is the not total soluble flavone of Guava Leaf and the measurement result figure of insolubility flavones content in be the same as Example.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited
In this.
Embodiment 1
(1) preparation of Guava Leaf matrix：The Guava Leaf cleaned up is put into 60 DEG C of oven for drying 16h, broken mistake is rubbed
Aperture is the sieve of 4 mesh, and the Guava Leaf of sieving is enzymatic hydrolysis matrix；Water, the consumption of water are added in enzymatic reaction matrix
(adjusting pH=5.5 with citric acid) is the 80% of gross weight；
(2) enzymatic hydrolysis reaction：Then the Guava Leaf finally given cellulase (8000U/g, similarly hereinafter) and step (1)
It is well mixed to be placed in triangular flask, under 50 DEG C of water-baths, after ferment treatment 6h, 80 DEG C of baking oven 10min (inactivation cellulase) are placed in,
It is cooled to room temperature；Then hemicellulase (8000U/g, similarly hereinafter) is added, is well mixed, under 50 DEG C of water-baths, 6h is digested, is placed in
80 DEG C of baking oven 10min (inactivation hemicellulase), are cooled to room temperature；Continue to add beta-glucosidase (8000U/g, under
Together), it is well mixed, under 50 DEG C of water-baths, digests 6h, be placed in 80 DEG C of baking oven 10min (inactivation beta-glucosidase)；Wherein, it is fine
The plain enzyme of dimension, hemicellulase, the quality consumption of beta-glucosidase are comparable to the guava for the sieving that step (1) is obtained respectively
The 0.5% of leaf (i.e. enzymatic hydrolysis matrix) quality.
(3) Guava Leaf product treatment after enzyme hydrolysis：Guava Leaf after a variety of enzyme hydrolysis is placed in 60 DEG C of drying of baking oven
16h, obtains the Guava Leaf product rich in soluble polyphenol and flavone aglycone.
Embodiment 2
(1) preparation of Guava Leaf matrix：Substantially identical with the step of embodiment 1 (1), difference is, pH is adjusted with citric acid
=5.5.
(2) multi-enzymatic hydrolysis react：Then the Guava Leaf finally given hemicellulase and step (1) is well mixed and put
In triangular flask, under 50 DEG C of water-baths, after ferment treatment 6h, 80 DEG C of baking oven 10min (inactivation hemicellulase) are placed in, room is cooled to
Temperature；Then cellulase is added, is well mixed, under 50 DEG C of water-baths, 6h is digested, 80 DEG C of baking oven 10min (inactivation celluloses are placed in
Enzyme), it is cooled to room temperature；Continue to add beta-glucosidase, be well mixed, under 50 DEG C of water-baths, digest 6h, be placed in 80 DEG C of bakings
Case 10min (inactivation beta-glucosidase)；Wherein, the quality consumption difference of hemicellulase, cellulase, beta-glucosidase
It is comparable to the 0.5% of Guava Leaf (the i.e. enzymatic hydrolysis matrix) quality for the sieving that step (1) is obtained.
(3) Guava Leaf product treatment after enzyme hydrolysis：Guava Leaf after a variety of enzyme hydrolysis is placed in 60 DEG C of drying of baking oven
16h, obtains the Guava Leaf product rich in soluble polyphenol and flavone aglycone.
Embodiment 3
(1) preparation of Guava Leaf matrix：It is identical with the step of embodiment 2 (1).
(2) a variety of enzymatic hydrolysis reactions：Then the Guava Leaf finally given zytase (8000U/g) and step (1)
It is well mixed to be placed in triangular flask, under 50 DEG C of water-baths, after ferment treatment 6h, 80 DEG C of baking oven 10min (inactivation zytase) are placed in,
It is cooled to room temperature；Then cellulase is added, is well mixed, under 50 DEG C of water-baths, 6h is digested, is placed in 80 DEG C of baking oven 10min and (goes out
Cellulase living), it is cooled to room temperature；Continue to add hemicellulase, be well mixed, under 50 DEG C of water-baths, digest 6h, be placed in
80 DEG C of baking oven 10min (inactivation hemicellulase)；Wherein, the quality consumption difference of zytase, cellulase, hemicellulase
It is comparable to the 0.5% of Guava Leaf (the i.e. enzymatic hydrolysis matrix) quality for the sieving that step (1) is obtained.
(3) Guava Leaf product treatment after enzyme hydrolysis：Guava Leaf after a variety of enzyme hydrolysis is placed in 60 DEG C of drying of baking oven
16h, obtains the Guava Leaf product rich in soluble polyphenol and flavone aglycone.
Embodiment 4
(1) preparation of Guava Leaf matrix：It is identical with the step of embodiment 2 (1).
(2) a variety of enzymatic hydrolysis reactions：Then the Guava Leaf finally given beta-glucosidase and step (1) mixes equal
It is even to be placed in triangular flask, under 50 DEG C of water-baths, after ferment treatment 6h, 80 DEG C of baking oven 10min (inactivation beta-glucosidase) are placed in, it is cold
But to room temperature；Then cellulase is added, is well mixed, under 50 DEG C of water-baths, 6h is digested, 80 DEG C of baking oven 10min (inactivations are placed in
Cellulase), it is cooled to room temperature；Continue to add hemicellulase, be well mixed, under 50 DEG C of water-baths, digest 6h, be placed in 80
DEG C baking oven 10min (inactivation hemicellulase)；Wherein, the quality consumption point of beta-glucosidase, cellulase, hemicellulase
The 0.5% of Guava Leaf (the i.e. enzymatic hydrolysis matrix) quality for the sieving that step (1) is obtained is not comparable to.
(3) Guava Leaf product treatment after enzyme hydrolysis：Guava Leaf after a variety of enzyme hydrolysis is placed in 60 DEG C of drying of baking oven
16h, obtains the Guava Leaf product rich in soluble polyphenol and flavone aglycone.
Effect example
First, detection method
Guava Leaf product and undressed Guava Leaf prepared by embodiment 1~4 is crushed with flour mill, is passed through
40 mesh sieves, extraction and detection for following composition：
1. soluble many phenol extractions：Guava Leaf product prepared by 1.0g embodiments 1~4 is taken respectively in 50mL colorimetric cylinders
In, 25mL 50% (v/v) methanol solution is added, after 45 DEG C of water-bath extraction 1h, is filtered with 0.45 μm of filter paper, filtrate passes through true
Empty Rotary Evaporators rotate 30min at 37 DEG C, remove methanol, obtain concentrate, and 40mL distilled water is added into concentrate,
Then 10mL hexane degreasings are added, then are extracted 3 times with 70mL ethyl acetate, combining extraction liquid, at 35 DEG C, vacuum is spin-dried for, and is gone
Except ethyl acetate.The dissolving of 5mL 50% (v/v) methanol is eventually adding, is soluble polyphenol extract solution.- 20 DEG C of preservations are placed in,
For polyphenol content analysis and HPLC quantitative analyses.
2. it is insoluble to bind many phenol extractions of state：Remaining Guava Leaf residue after the step 1. soluble polyphenol of middle extraction is added
Enter 40mL distilled water and remove organic solvent, be filtered dry, in 60 DEG C of drying to constant weight, record the weight of residue.Add 40mL 4M
NaOH solution, extracts 4h at room temperature, then adjusts pH to 2 or so with concentrated hydrochloric acid (concentration is 37%), adds 70mL ethyl acetate
Extraction 3 times, combining extraction liquid, at 35 DEG C, vacuum is spin-dried for, and removes ethyl acetate, is eventually adding the dissolving of the methanol of 5mL 50%, i.e.,
For insoluble binding state polyphenol extract solution.- 20 DEG C of preservations are placed in, for polyphenol content analysis and HPLC quantitative analyses.
3. the detection of polyphenol content：The soluble polyphenol and insolubility binding state for drawing 100 μ L said extracteds respectively are more
Phenol extraction liquid, is diluted to suitable concn.Take 1mL to dilute sample liquid or gallic acid titer (10-100 μ g/mL), sequentially add
0.5mL forint phenol reagent is mixed, and is reacted 3-8min, is added 1.5mL20% (w/v) Na2CO3Solution, adds water and is settled to 10mL,
Fully vibration is mixed, and stands 30min.Compareed with blank reagent, determine the light absorption value under 760nm.
4. the measure of flavones content：The soluble polyphenol and insolubility for drawing 100 μ L said extracteds respectively bind state
Polyphenol extract solution, is diluted to suitable concn.Take 1mL to dilute sample liquid or rutin titer (10-100 μ g/mL), sequentially add
0.3mL 5% (w/v) NaNO2Solution is mixed, and stands 5min.Add 0.3mL 10% (w/v) AlCl3Solution is mixed, and is stood
6min.The mixing of 2mL 4% (w/v) NaOH solution is added, plus 70%, (v/v) ethanol solution is settled to 10mL, fully vibration, it is quiet
Put 10min.Compareed with blank reagent, determine the light absorption value under 510nm.
5. flavone aglycone (Quercetin and kaempferol) is detected：Draw respectively by the soluble polyphenol of said extracted with it is insoluble
Property binding state polyphenol extract solution filter with 0.45 μm of filter paper, take clear liquid to cross 0.22 μm of organic miillpore filter, filtrate progress HPLC divides
Analysis.Concrete analysis condition is：The highly effective liquid phase chromatographic system (Waters 2695) of UV-detector (Waters 2998), detection
Wavelength 350nm, 30 DEG C of column temperature, C18 chromatographic columns.Mobile phase used is：A-0.1% (v/v) aqueous formic acid, B- acetonitriles are molten
Liquid, flow velocity is 0.8mL/min, the μ L of sample size 10.Testing conditions：Gradient elution -0min, 85%A+15%B, 5min, 85%A+
15%B, 10min, 80%A+20%B, 20min, 65%A+35%B, 30min, 50%A+50%B, 31min, 20%A+80%
B, 40min, 20%A+80%B, 45min, 85%A+15%B, 50min, 85%A+15%B (being volume ratio).Analysis time
For 50min.
5. oxidation resistance is detected：
a：DPPH radical scavenging activities
The soluble polyphenol and insolubility for drawing 100 μ L said extracteds respectively bind state polyphenol extract solution, are diluted to conjunction
Suitable concentration.Take 100 μ L to dilute sample liquid or vitamin C titer (5-30 μ g/mL), add 400 μ L DPPH- methanol reagents, in
30 DEG C, dark place standing 30min.Negative control is done with water, VC determines the light absorption value under 510nm as positive control.Sample
DPPH radical scavenging activities are represented with VC, i.e., per mmol/L number of the g Guava Leafs sample equivalent to VC.
b：ABTS+Radical scavenging activity
The soluble polyphenol and insolubility for drawing 100 μ L said extracteds respectively bind state polyphenol extract solution, are diluted to conjunction
Suitable concentration.Take 50 μ L to dilute sample liquid or vitamin C titer (5-30 μ g/mL), add 400 μ L ABTS+(7mM ABTS with
2.45mM K2S2O8With 2:1 volume ratio is mixed, and dark stands 16h) reagent, in 30 DEG C, 30min is stood at dark.The moon is done with water
Property control, VC determines the light absorption value under 510nm as positive control.The ABTS of sample+Radical scavenging activity represents with VC,
I.e. per mmol/L number of the g Guava Leafs sample equivalent to VC.
6. oxidation resistance is detected：
Take respectively the soluble polyphenol of above-mentioned 2 μ L dilution and insolubility binding state polyphenol extract solution sample liquid (2mg/mL) and
Quercetin titer (2mg/mL), adds 5 μ L pMD 18-T DNAs (200ng/ μ L), 10 μ L Fenton reagents (50mM
VC、80mM FeCl3And 30mM H2O2), mixed with liquid-transfering gun, it is dark at 37 DEG C to stand 30min.Done with PBS
Then mixed liquor is loaded onto the DNA glue after 1% agarose gel electrophoresis, electrophoresis by blank control, Quercetin as positive control
Observed under the conditions of ultraviolet, calculate the ratio that helical form DNA accounts for STb gene.DNA damage inhibiting rate calculation formula is as follows：
2nd, testing result
As a result as illustrated in fig. 1 and 2, find solvable according to the Guava Leaf product after Examples 1 and 2 order addition ferment treatment
Property polyphenol content substantially increases, and soluble flavone content also substantially increases.And the enzyme reaction of embodiment 1 order is according to cellulose
Guava Leaf solubility polyphenol content highest (treated kind of embodiment 1 after enzyme, hemicellulase and beta-glucosidase ferment treatment
Pomegranate leaf improves 94.74% relative to the soluble polyphenol content of untreated fish group, and soluble flavone improves 89.48%, and insoluble
Binding state polyphenol content substantially reduce.This illustrates that a variety of ferment treatments can promote the soluble polyphenol release of Guava Leaf).And use
It is worst that embodiment 3 sequentially adds polyphenol release efficiency after zytase, cellulase, a variety of enzymes of hemicellulase；But according to
Embodiment 4 first uses beta-glucosidase ferment treatment, then with treated Guava Leaf relative to the soluble polyphenol of untreated fish group acquisition
Efficiency is poor compared with embodiment 1,2, but is better than embodiment 3.
Flavone aglycone (Quercetin and Kaempferol) content is detected using high performance liquid chromatography.Added using the order of embodiment 1
Add after kind of enzyme cohydrolysis, the content highest of Quercetin and Kaempferol, respectively 248.95mg/100g DM, 11.35mg/
100g DM, relative to untreated fish group, are respectively increased 1.97 times, 1.82 times.And Guava Leaf after embodiment 1, more than 2 kinds of ferment treatment
Soluble polyphenol extract solution total antioxidant activity and DNA damage inhibitory action is significantly improved, wherein embodiment 1 is by a variety of
Guava Leaf biology after enzyme (0.5% cellulase, 0.5% hemicellulase, and 0.5% beta-glucosidase) processing is living
Property highest.DPPH、ABTS+Radical scavenging activity is equivalent to 74.29mmol VC/g DM, 77.41mmol VC/g DM.It is right
The inhibiting rate of DNA damage has reached 81.23%.And the Guava Leaf activity after the processing of the method for embodiment 3 is minimum.Detection data are shown in
Table 1.
Table 1
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of preparation method of the Guava Leaf rich in soluble polyphenol and flavone aglycone, it is characterised in that including following step
Suddenly：
(1) Guava Leaf cleaned up drained, dried, rubbing broken, sieving, removed Guava Leaf stem position, obtain size
Basically identical Guava Leaf position；
(2) after the Guava Leaf position for finally giving step (1) is mixed with water, pH value is regulated, enzyme is added, is digested
Reaction；
(3) step (2) is carried out to the system after enzyme digestion reaction to dry, obtains kind stone rich in soluble polyphenol and flavone aglycone
Pomegranate leaf product.
2. the preparation method of the Guava Leaf according to claim 1 rich in soluble polyphenol and flavone aglycone, its feature
It is：
Enzyme described in step (2) is at least one in cellulase, hemicellulase, beta-glucosidase and zytase
Kind.
3. the preparation method of the Guava Leaf according to claim 2 rich in soluble polyphenol and flavone aglycone, its feature
It is：
Enzyme described in step (2) is the combination of cellulase, hemicellulase and beta-glucosidase.
4. the preparation method of the Guava Leaf according to claim 2 rich in soluble polyphenol and flavone aglycone, its feature
It is：
Described cellulase be enzyme activity be 8000U/g cellulase；
Described hemicellulase be enzyme activity be 8000U/g hemicellulase；
Described beta-glucosidase be enzyme activity be 8000U/g beta-glucosidase；
Described zytase be enzyme activity be 8000U/g zytase；
0.5% of the quality consumption of described cellulase equivalent to Guava Leaf position quality；
0.5% of the quality consumption of described hemicellulase equivalent to Guava Leaf position quality；
0.5% of the quality consumption of described beta-glucosidase equivalent to Guava Leaf position quality；
0.5% of the quality consumption of described zytase equivalent to Guava Leaf position quality.
5. the preparation method of the Guava Leaf according to claim 1 rich in soluble polyphenol and flavone aglycone, its feature
It is：
The condition of drying described in step (1) is in 50~80 DEG C of drying to constant weight；
The temperature of drying described in step (3) is 50~70 DEG C.
6. the preparation method of the Guava Leaf according to claim 1 rich in soluble polyphenol and flavone aglycone, its feature
It is：
PH value described in step (2) is 4.5~6.0；
The temperature of enzyme digestion reaction described in step (2) is 45~55 DEG C；
The time of enzyme digestion reaction described in step (2) is based on 5~8h of each enzyme reaction.
7. the preparation method of the Guava Leaf according to claim 1 rich in soluble polyphenol and flavone aglycone, its feature
It is：
The detailed process of described enzyme digestion reaction such as step 1), 2) or 3) shown in：
1) first add cellulase and carry out first time enzymolysis, inactivate cellulase；Add hemicellulase and carry out second of enzyme
Solution, inactivates hemicellulase；It is eventually adding beta-glucosidase and carries out third time enzymolysis, inactivates beta-glucosidase；
2) first add hemicellulase and carry out first time enzymolysis, inactivate hemicellulase；Cellulase is added to carry out second
Enzymolysis, inactivates cellulase；It is eventually adding beta-glucosidase and carries out third time enzymolysis, inactivates beta-glucosidase；
3) first add beta-glucosidase and carry out first time enzymolysis, inactivate beta-glucosidase；Add cellulase and carry out the
Secondary enzymolysis, inactivates cellulase；It is eventually adding hemicellulase and carries out third time enzymolysis, inactivates hemicellulase.
8. the preparation method of the Guava Leaf according to claim 7 rich in soluble polyphenol and flavone aglycone, its feature
It is：
Step 1), 2) and 3) in,
The reaction condition that described first time enzymolysis, for the second time enzymolysis and third time is digested is respectively in 50 DEG C of reaction 6h；
The condition of described inactivation is 80 DEG C of processing 10min；
The quality consumption of described cellulase is equivalent to the 0.5% of Guava Leaf position quality；
The quality consumption of described hemicellulase is equivalent to the 0.5% of Guava Leaf position quality；
The quality consumption of described beta-glucosidase is equivalent to the 0.5% of Guava Leaf position quality.
9. a kind of Guava Leaf product rich in soluble polyphenol and flavone aglycone, it is characterised in that：By claim 1~
Preparation method described in 8 any one is obtained.
10. the Guava Leaf product rich in soluble polyphenol and flavone aglycone described in claim 9 in field of food and/or
Application in field of health care products.
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| CN110755459A (en) * | 2019-11-22 | 2020-02-07 | 广西南亚热带农业科学研究所 | Extraction process of annona squamosa leaf polyphenol |
| CN113481242A (en) * | 2021-07-19 | 2021-10-08 | 福建省农业科学院亚热带农业研究所（福建省农业科学院蔗麻研究中心） | Method for improving biological activity of plant polyphenol through microbial transformation |
| CN118576628A (en) * | 2024-07-29 | 2024-09-03 | 四川淇力康生物科技有限公司 | Method for extracting total flavonoids from sea buckthorn leaves |
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