CN107252093A - It is a kind of to be rich in soluble polyphenol and the Guava Leaf and preparation method of flavone aglycone and application - Google Patents

It is a kind of to be rich in soluble polyphenol and the Guava Leaf and preparation method of flavone aglycone and application Download PDF

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CN107252093A
CN107252093A CN201710383696.4A CN201710383696A CN107252093A CN 107252093 A CN107252093 A CN 107252093A CN 201710383696 A CN201710383696 A CN 201710383696A CN 107252093 A CN107252093 A CN 107252093A
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guava leaf
rich
hemicellulase
glucosidase
cellulase
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CN107252093B (en
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吴振强
王露
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South China University of Technology SCUT
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South China University of Technology SCUT
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Priority to PCT/CN2017/113883 priority patent/WO2018214458A1/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Mycology (AREA)
  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The present invention discloses a kind of rich in soluble polyphenol and the Guava Leaf and preparation method of flavone aglycone and application.The present invention obtains Guava Leaf position by the way that the Guava Leaf cleaned up to be drained to, dried, rubbed broken, sieving;After it is mixed with water again, pH value is regulated, enzyme is added, enzyme digestion reaction is carried out;Terminate the system drying of enzyme digestion reaction, obtain the Guava Leaf product rich in soluble polyphenol and flavone aglycone.The soluble polyphenol content of guava leaf tea obtained by the preparation method is greatly improved, and be the stronger flavone aglycone composition of functional activity by the flavone glycoside ingredient degradation of Guava Leaf, Quercetin, the Aglycones content such as Kaempferol are improved, improves that Guava Leaf is anti-oxidant and anti-DNA damnification effect.So as to which the potentiality that should be applied rich in soluble polyphenol and flavone aglycone Guava Leaf in field of food and/or field of health care products are big.

Description

A kind of Guava Leaf and preparation method rich in soluble polyphenol and flavone aglycone with Using
Technical field
The invention belongs to field of food, more particularly to a kind of Guava Leaf rich in soluble polyphenol and flavone aglycone and Preparation method and application.
Background technology
Guava Leaf has usage history for many years as a kind of material of medicine-food two-purpose, with anti-oxidant, suppression DNA Damage, hypoglycemic, anti-inflammatory, antibacterial, hypotensive, a variety of curative effects such as cardioprotection.Many researchs show main in Guava Leaf Bioactive functions composition includes polyphenols, and these active materials can be eliminated to be drawn by internal excess oxygen or nitrogen free radical The impaired caused body injury of Antioxidative Defense System risen.Due to plant polyphenol it is main with three kinds of forms (free state, Conjugation state and binding state) be present in plant, and bind state polyphenol generally with the polysaccharide on plant cell wall, protein with Chemical bond form is combined, it is difficult to be extracted, causes guava leaf polyphenol class active material utilization low.
Therefore, it is necessary to promote soluble polyphenol and the release of flavone aglycone of Guava Leaf, so as to make full use of a kind stone Pomegranate leaf Polyphenols active material.
The content of the invention
The primary and foremost purpose of the present invention be to overcome the shortcoming and deficiency of prior art there is provided one kind rich in soluble polyphenol with And the preparation method of the Guava Leaf of flavone aglycone.
Another object of the present invention is to provide the Guava Leaf product obtained by above-mentioned preparation method.
It is still another object of the present invention to provide the application of described Guava Leaf product.
The purpose of the present invention is achieved through the following technical solutions:A kind of kind stone rich in soluble polyphenol and flavone aglycone The preparation method of pomegranate leaf, comprises the following steps:
(1) Guava Leaf cleaned up drained, dried, rubbing broken, broken Guava Leaf will be rubbed and sieved, guava is removed Base of leaf cadre position, obtains the basically identical Guava Leaf position of size;
(2) after the Guava Leaf position for finally giving step (1) is mixed with water, pH value is regulated, enzyme is added, carried out Enzyme digestion reaction;
(3) step (2) is carried out to the system after enzyme digestion reaction to dry, obtained rich in soluble polyphenol and flavone aglycone Guava Leaf product.
The condition of drying described in step (1) is preferably in 50~80 DEG C of drying to constant weight;More preferably in 60 DEG C of bakings Do to constant weight.
The preferential sieve for selecting aperture for 4 mesh of sieving described in step (1).
The consumption of water described in step (2), which is that the Guava Leaf for finally giving step (1) is scattered, to be advisable, to be conducive to Carry out enzyme digestion reaction;4 times of the Guava Leaf quality preferably finally given equivalent to step (1).
PH value described in step (2) is 4.5~6.0;Preferably 5~5.5.
The temperature of enzyme digestion reaction described in step (2) is 45~55 DEG C;Preferably 50 DEG C.
The time of enzyme digestion reaction described in step (2) is preferably based on 5~8h of each enzyme reaction;It is more preferably each Plant enzyme reaction 6h meters.
Enzyme described in step (2) be in cellulase, hemicellulase, beta-glucosidase and zytase at least It is a kind of;Preferably cellulase, hemicellulase and beta-glucosidase is applied in combination.
Described cellulase be preferably enzyme activity be 8000U/g cellulase.
Described hemicellulase be preferably enzyme activity be 8000U/g hemicellulase.
Described beta-glucosidase be preferably enzyme activity be 8000U/g beta-glucosidase.
Described zytase be preferably enzyme activity be 8000U/g zytase.
The quality consumption of described cellulase is preferably equivalent to the 0.5% of Guava Leaf position quality.
The quality consumption of described hemicellulase is preferably equivalent to the 0.5% of Guava Leaf position quality.
The quality consumption of described beta-glucosidase is preferably equivalent to the 0.5% of Guava Leaf position quality.
The quality consumption of described zytase is preferably equivalent to the 0.5% of Guava Leaf position quality.
The detailed process of described enzyme digestion reaction is preferably such as step 1), 2) or 3) shown in, most preferably step 1):
1) first add cellulase and carry out first time enzymolysis, inactivate cellulase;Add hemicellulase and carry out second Secondary enzymolysis, inactivates hemicellulase;It is eventually adding beta-glucosidase and carries out third time enzymolysis, inactivates beta-glucosidase;
2) first add hemicellulase and carry out first time enzymolysis, inactivate hemicellulase;Add cellulase and carry out the Secondary enzymolysis, inactivates cellulase;It is eventually adding beta-glucosidase and carries out third time enzymolysis, inactivates beta-glucosidase;
3) first add beta-glucosidase and carry out first time enzymolysis, inactivate beta-glucosidase;Cellulase is added to enter Second of enzymolysis of row, inactivates cellulase;It is eventually adding hemicellulase and carries out third time enzymolysis, inactivates hemicellulase.
Step 1), 2) and 3) in,
The reaction condition that described first time enzymolysis, for the second time enzymolysis and third time is digested is respectively preferably in 50 DEG C of reactions 6h;
The condition of described inactivation is preferably 80 DEG C of processing 10min;
The quality consumption of described cellulase is preferably equivalent to the 0.5% of Guava Leaf position quality;
The quality consumption of described hemicellulase is preferably equivalent to the 0.5% of Guava Leaf position quality;
The quality consumption of described beta-glucosidase is preferably equivalent to the 0.5% of Guava Leaf position quality.
The temperature of drying described in step (3) is preferably 50~70 DEG C;More preferably 60 DEG C.
The time preferably at least 12h of drying described in step (3);More preferably 16h.
A kind of Guava Leaf product rich in soluble polyphenol and flavone aglycone, is obtained by above-mentioned preparation method.
The described Guava Leaf product rich in soluble polyphenol and flavone aglycone is led in field of food and/or health products Applied in domain;Its is direct-edible;Also varieties of food items can be further processed into, such as rich in soluble polyphenol and flavone aglycone Guava Leaf tea beverage, the Guava Leaf biscuit rich in soluble polyphenol and flavone aglycone, nutritious food bar etc..
The present invention has the following advantages and effect relative to prior art:
The preparation method that the present invention is provided, is that will be difficult to extract in Guava Leaf by a variety of enzyme hydrolysis, insoluble The release of state polyphenol is bound, is changed into easy extraction, soluble polyphenol;And the macromolecular functional component of Guava Leaf is degraded to absorption Ability is stronger, the Aglycones content such as the higher small molecule Quercetin of functional activity and Kaempferol;And the enzyme digestion reaction time is short, condition Gently, efficiency high, can be used for medicinal plant processing synergy.The preparation method lifts Guava Leaf product oxidation resistance, suppression DNA damage ability processed, reduces blood glucose, and cholesterol, prevention cardiovascular and cerebrovascular disease etc. is acted on.
Brief description of the drawings
Fig. 1 is the measurement result figure of the not total soluble polyphenol of Guava Leaf and insolubility polyphenol content in be the same as Example.
Fig. 2 is the not total soluble flavone of Guava Leaf and the measurement result figure of insolubility flavones content in be the same as Example.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited In this.
Embodiment 1
(1) preparation of Guava Leaf matrix:The Guava Leaf cleaned up is put into 60 DEG C of oven for drying 16h, broken mistake is rubbed Aperture is the sieve of 4 mesh, and the Guava Leaf of sieving is enzymatic hydrolysis matrix;Water, the consumption of water are added in enzymatic reaction matrix (adjusting pH=5.5 with citric acid) is the 80% of gross weight;
(2) enzymatic hydrolysis reaction:Then the Guava Leaf finally given cellulase (8000U/g, similarly hereinafter) and step (1) It is well mixed to be placed in triangular flask, under 50 DEG C of water-baths, after ferment treatment 6h, 80 DEG C of baking oven 10min (inactivation cellulase) are placed in, It is cooled to room temperature;Then hemicellulase (8000U/g, similarly hereinafter) is added, is well mixed, under 50 DEG C of water-baths, 6h is digested, is placed in 80 DEG C of baking oven 10min (inactivation hemicellulase), are cooled to room temperature;Continue to add beta-glucosidase (8000U/g, under Together), it is well mixed, under 50 DEG C of water-baths, digests 6h, be placed in 80 DEG C of baking oven 10min (inactivation beta-glucosidase);Wherein, it is fine The plain enzyme of dimension, hemicellulase, the quality consumption of beta-glucosidase are comparable to the guava for the sieving that step (1) is obtained respectively The 0.5% of leaf (i.e. enzymatic hydrolysis matrix) quality.
(3) Guava Leaf product treatment after enzyme hydrolysis:Guava Leaf after a variety of enzyme hydrolysis is placed in 60 DEG C of drying of baking oven 16h, obtains the Guava Leaf product rich in soluble polyphenol and flavone aglycone.
Embodiment 2
(1) preparation of Guava Leaf matrix:Substantially identical with the step of embodiment 1 (1), difference is, pH is adjusted with citric acid =5.5.
(2) multi-enzymatic hydrolysis react:Then the Guava Leaf finally given hemicellulase and step (1) is well mixed and put In triangular flask, under 50 DEG C of water-baths, after ferment treatment 6h, 80 DEG C of baking oven 10min (inactivation hemicellulase) are placed in, room is cooled to Temperature;Then cellulase is added, is well mixed, under 50 DEG C of water-baths, 6h is digested, 80 DEG C of baking oven 10min (inactivation celluloses are placed in Enzyme), it is cooled to room temperature;Continue to add beta-glucosidase, be well mixed, under 50 DEG C of water-baths, digest 6h, be placed in 80 DEG C of bakings Case 10min (inactivation beta-glucosidase);Wherein, the quality consumption difference of hemicellulase, cellulase, beta-glucosidase It is comparable to the 0.5% of Guava Leaf (the i.e. enzymatic hydrolysis matrix) quality for the sieving that step (1) is obtained.
(3) Guava Leaf product treatment after enzyme hydrolysis:Guava Leaf after a variety of enzyme hydrolysis is placed in 60 DEG C of drying of baking oven 16h, obtains the Guava Leaf product rich in soluble polyphenol and flavone aglycone.
Embodiment 3
(1) preparation of Guava Leaf matrix:It is identical with the step of embodiment 2 (1).
(2) a variety of enzymatic hydrolysis reactions:Then the Guava Leaf finally given zytase (8000U/g) and step (1) It is well mixed to be placed in triangular flask, under 50 DEG C of water-baths, after ferment treatment 6h, 80 DEG C of baking oven 10min (inactivation zytase) are placed in, It is cooled to room temperature;Then cellulase is added, is well mixed, under 50 DEG C of water-baths, 6h is digested, is placed in 80 DEG C of baking oven 10min and (goes out Cellulase living), it is cooled to room temperature;Continue to add hemicellulase, be well mixed, under 50 DEG C of water-baths, digest 6h, be placed in 80 DEG C of baking oven 10min (inactivation hemicellulase);Wherein, the quality consumption difference of zytase, cellulase, hemicellulase It is comparable to the 0.5% of Guava Leaf (the i.e. enzymatic hydrolysis matrix) quality for the sieving that step (1) is obtained.
(3) Guava Leaf product treatment after enzyme hydrolysis:Guava Leaf after a variety of enzyme hydrolysis is placed in 60 DEG C of drying of baking oven 16h, obtains the Guava Leaf product rich in soluble polyphenol and flavone aglycone.
Embodiment 4
(1) preparation of Guava Leaf matrix:It is identical with the step of embodiment 2 (1).
(2) a variety of enzymatic hydrolysis reactions:Then the Guava Leaf finally given beta-glucosidase and step (1) mixes equal It is even to be placed in triangular flask, under 50 DEG C of water-baths, after ferment treatment 6h, 80 DEG C of baking oven 10min (inactivation beta-glucosidase) are placed in, it is cold But to room temperature;Then cellulase is added, is well mixed, under 50 DEG C of water-baths, 6h is digested, 80 DEG C of baking oven 10min (inactivations are placed in Cellulase), it is cooled to room temperature;Continue to add hemicellulase, be well mixed, under 50 DEG C of water-baths, digest 6h, be placed in 80 DEG C baking oven 10min (inactivation hemicellulase);Wherein, the quality consumption point of beta-glucosidase, cellulase, hemicellulase The 0.5% of Guava Leaf (the i.e. enzymatic hydrolysis matrix) quality for the sieving that step (1) is obtained is not comparable to.
(3) Guava Leaf product treatment after enzyme hydrolysis:Guava Leaf after a variety of enzyme hydrolysis is placed in 60 DEG C of drying of baking oven 16h, obtains the Guava Leaf product rich in soluble polyphenol and flavone aglycone.
Effect example
First, detection method
Guava Leaf product and undressed Guava Leaf prepared by embodiment 1~4 is crushed with flour mill, is passed through 40 mesh sieves, extraction and detection for following composition:
1. soluble many phenol extractions:Guava Leaf product prepared by 1.0g embodiments 1~4 is taken respectively in 50mL colorimetric cylinders In, 25mL 50% (v/v) methanol solution is added, after 45 DEG C of water-bath extraction 1h, is filtered with 0.45 μm of filter paper, filtrate passes through true Empty Rotary Evaporators rotate 30min at 37 DEG C, remove methanol, obtain concentrate, and 40mL distilled water is added into concentrate, Then 10mL hexane degreasings are added, then are extracted 3 times with 70mL ethyl acetate, combining extraction liquid, at 35 DEG C, vacuum is spin-dried for, and is gone Except ethyl acetate.The dissolving of 5mL 50% (v/v) methanol is eventually adding, is soluble polyphenol extract solution.- 20 DEG C of preservations are placed in, For polyphenol content analysis and HPLC quantitative analyses.
2. it is insoluble to bind many phenol extractions of state:Remaining Guava Leaf residue after the step 1. soluble polyphenol of middle extraction is added Enter 40mL distilled water and remove organic solvent, be filtered dry, in 60 DEG C of drying to constant weight, record the weight of residue.Add 40mL 4M NaOH solution, extracts 4h at room temperature, then adjusts pH to 2 or so with concentrated hydrochloric acid (concentration is 37%), adds 70mL ethyl acetate Extraction 3 times, combining extraction liquid, at 35 DEG C, vacuum is spin-dried for, and removes ethyl acetate, is eventually adding the dissolving of the methanol of 5mL 50%, i.e., For insoluble binding state polyphenol extract solution.- 20 DEG C of preservations are placed in, for polyphenol content analysis and HPLC quantitative analyses.
3. the detection of polyphenol content:The soluble polyphenol and insolubility binding state for drawing 100 μ L said extracteds respectively are more Phenol extraction liquid, is diluted to suitable concn.Take 1mL to dilute sample liquid or gallic acid titer (10-100 μ g/mL), sequentially add 0.5mL forint phenol reagent is mixed, and is reacted 3-8min, is added 1.5mL20% (w/v) Na2CO3Solution, adds water and is settled to 10mL, Fully vibration is mixed, and stands 30min.Compareed with blank reagent, determine the light absorption value under 760nm.
4. the measure of flavones content:The soluble polyphenol and insolubility for drawing 100 μ L said extracteds respectively bind state Polyphenol extract solution, is diluted to suitable concn.Take 1mL to dilute sample liquid or rutin titer (10-100 μ g/mL), sequentially add 0.3mL 5% (w/v) NaNO2Solution is mixed, and stands 5min.Add 0.3mL 10% (w/v) AlCl3Solution is mixed, and is stood 6min.The mixing of 2mL 4% (w/v) NaOH solution is added, plus 70%, (v/v) ethanol solution is settled to 10mL, fully vibration, it is quiet Put 10min.Compareed with blank reagent, determine the light absorption value under 510nm.
5. flavone aglycone (Quercetin and kaempferol) is detected:Draw respectively by the soluble polyphenol of said extracted with it is insoluble Property binding state polyphenol extract solution filter with 0.45 μm of filter paper, take clear liquid to cross 0.22 μm of organic miillpore filter, filtrate progress HPLC divides Analysis.Concrete analysis condition is:The highly effective liquid phase chromatographic system (Waters 2695) of UV-detector (Waters 2998), detection Wavelength 350nm, 30 DEG C of column temperature, C18 chromatographic columns.Mobile phase used is:A-0.1% (v/v) aqueous formic acid, B- acetonitriles are molten Liquid, flow velocity is 0.8mL/min, the μ L of sample size 10.Testing conditions:Gradient elution -0min, 85%A+15%B, 5min, 85%A+ 15%B, 10min, 80%A+20%B, 20min, 65%A+35%B, 30min, 50%A+50%B, 31min, 20%A+80% B, 40min, 20%A+80%B, 45min, 85%A+15%B, 50min, 85%A+15%B (being volume ratio).Analysis time For 50min.
5. oxidation resistance is detected:
a:DPPH radical scavenging activities
The soluble polyphenol and insolubility for drawing 100 μ L said extracteds respectively bind state polyphenol extract solution, are diluted to conjunction Suitable concentration.Take 100 μ L to dilute sample liquid or vitamin C titer (5-30 μ g/mL), add 400 μ L DPPH- methanol reagents, in 30 DEG C, dark place standing 30min.Negative control is done with water, VC determines the light absorption value under 510nm as positive control.Sample DPPH radical scavenging activities are represented with VC, i.e., per mmol/L number of the g Guava Leafs sample equivalent to VC.
b:ABTS+Radical scavenging activity
The soluble polyphenol and insolubility for drawing 100 μ L said extracteds respectively bind state polyphenol extract solution, are diluted to conjunction Suitable concentration.Take 50 μ L to dilute sample liquid or vitamin C titer (5-30 μ g/mL), add 400 μ L ABTS+(7mM ABTS with 2.45mM K2S2O8With 2:1 volume ratio is mixed, and dark stands 16h) reagent, in 30 DEG C, 30min is stood at dark.The moon is done with water Property control, VC determines the light absorption value under 510nm as positive control.The ABTS of sample+Radical scavenging activity represents with VC, I.e. per mmol/L number of the g Guava Leafs sample equivalent to VC.
6. oxidation resistance is detected:
Take respectively the soluble polyphenol of above-mentioned 2 μ L dilution and insolubility binding state polyphenol extract solution sample liquid (2mg/mL) and Quercetin titer (2mg/mL), adds 5 μ L pMD 18-T DNAs (200ng/ μ L), 10 μ L Fenton reagents (50mM VC、80mM FeCl3And 30mM H2O2), mixed with liquid-transfering gun, it is dark at 37 DEG C to stand 30min.Done with PBS Then mixed liquor is loaded onto the DNA glue after 1% agarose gel electrophoresis, electrophoresis by blank control, Quercetin as positive control Observed under the conditions of ultraviolet, calculate the ratio that helical form DNA accounts for STb gene.DNA damage inhibiting rate calculation formula is as follows:
2nd, testing result
As a result as illustrated in fig. 1 and 2, find solvable according to the Guava Leaf product after Examples 1 and 2 order addition ferment treatment Property polyphenol content substantially increases, and soluble flavone content also substantially increases.And the enzyme reaction of embodiment 1 order is according to cellulose Guava Leaf solubility polyphenol content highest (treated kind of embodiment 1 after enzyme, hemicellulase and beta-glucosidase ferment treatment Pomegranate leaf improves 94.74% relative to the soluble polyphenol content of untreated fish group, and soluble flavone improves 89.48%, and insoluble Binding state polyphenol content substantially reduce.This illustrates that a variety of ferment treatments can promote the soluble polyphenol release of Guava Leaf).And use It is worst that embodiment 3 sequentially adds polyphenol release efficiency after zytase, cellulase, a variety of enzymes of hemicellulase;But according to Embodiment 4 first uses beta-glucosidase ferment treatment, then with treated Guava Leaf relative to the soluble polyphenol of untreated fish group acquisition Efficiency is poor compared with embodiment 1,2, but is better than embodiment 3.
Flavone aglycone (Quercetin and Kaempferol) content is detected using high performance liquid chromatography.Added using the order of embodiment 1 Add after kind of enzyme cohydrolysis, the content highest of Quercetin and Kaempferol, respectively 248.95mg/100g DM, 11.35mg/ 100g DM, relative to untreated fish group, are respectively increased 1.97 times, 1.82 times.And Guava Leaf after embodiment 1, more than 2 kinds of ferment treatment Soluble polyphenol extract solution total antioxidant activity and DNA damage inhibitory action is significantly improved, wherein embodiment 1 is by a variety of Guava Leaf biology after enzyme (0.5% cellulase, 0.5% hemicellulase, and 0.5% beta-glucosidase) processing is living Property highest.DPPH、ABTS+Radical scavenging activity is equivalent to 74.29mmol VC/g DM, 77.41mmol VC/g DM.It is right The inhibiting rate of DNA damage has reached 81.23%.And the Guava Leaf activity after the processing of the method for embodiment 3 is minimum.Detection data are shown in Table 1.
Table 1
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification, Equivalent substitute mode is should be, is included within protection scope of the present invention.

Claims (10)

1. a kind of preparation method of the Guava Leaf rich in soluble polyphenol and flavone aglycone, it is characterised in that including following step Suddenly:
(1) Guava Leaf cleaned up drained, dried, rubbing broken, sieving, removed Guava Leaf stem position, obtain size Basically identical Guava Leaf position;
(2) after the Guava Leaf position for finally giving step (1) is mixed with water, pH value is regulated, enzyme is added, is digested Reaction;
(3) step (2) is carried out to the system after enzyme digestion reaction to dry, obtains kind stone rich in soluble polyphenol and flavone aglycone Pomegranate leaf product.
2. the preparation method of the Guava Leaf according to claim 1 rich in soluble polyphenol and flavone aglycone, its feature It is:
Enzyme described in step (2) is at least one in cellulase, hemicellulase, beta-glucosidase and zytase Kind.
3. the preparation method of the Guava Leaf according to claim 2 rich in soluble polyphenol and flavone aglycone, its feature It is:
Enzyme described in step (2) is the combination of cellulase, hemicellulase and beta-glucosidase.
4. the preparation method of the Guava Leaf according to claim 2 rich in soluble polyphenol and flavone aglycone, its feature It is:
Described cellulase be enzyme activity be 8000U/g cellulase;
Described hemicellulase be enzyme activity be 8000U/g hemicellulase;
Described beta-glucosidase be enzyme activity be 8000U/g beta-glucosidase;
Described zytase be enzyme activity be 8000U/g zytase;
0.5% of the quality consumption of described cellulase equivalent to Guava Leaf position quality;
0.5% of the quality consumption of described hemicellulase equivalent to Guava Leaf position quality;
0.5% of the quality consumption of described beta-glucosidase equivalent to Guava Leaf position quality;
0.5% of the quality consumption of described zytase equivalent to Guava Leaf position quality.
5. the preparation method of the Guava Leaf according to claim 1 rich in soluble polyphenol and flavone aglycone, its feature It is:
The condition of drying described in step (1) is in 50~80 DEG C of drying to constant weight;
The temperature of drying described in step (3) is 50~70 DEG C.
6. the preparation method of the Guava Leaf according to claim 1 rich in soluble polyphenol and flavone aglycone, its feature It is:
PH value described in step (2) is 4.5~6.0;
The temperature of enzyme digestion reaction described in step (2) is 45~55 DEG C;
The time of enzyme digestion reaction described in step (2) is based on 5~8h of each enzyme reaction.
7. the preparation method of the Guava Leaf according to claim 1 rich in soluble polyphenol and flavone aglycone, its feature It is:
The detailed process of described enzyme digestion reaction such as step 1), 2) or 3) shown in:
1) first add cellulase and carry out first time enzymolysis, inactivate cellulase;Add hemicellulase and carry out second of enzyme Solution, inactivates hemicellulase;It is eventually adding beta-glucosidase and carries out third time enzymolysis, inactivates beta-glucosidase;
2) first add hemicellulase and carry out first time enzymolysis, inactivate hemicellulase;Cellulase is added to carry out second Enzymolysis, inactivates cellulase;It is eventually adding beta-glucosidase and carries out third time enzymolysis, inactivates beta-glucosidase;
3) first add beta-glucosidase and carry out first time enzymolysis, inactivate beta-glucosidase;Add cellulase and carry out the Secondary enzymolysis, inactivates cellulase;It is eventually adding hemicellulase and carries out third time enzymolysis, inactivates hemicellulase.
8. the preparation method of the Guava Leaf according to claim 7 rich in soluble polyphenol and flavone aglycone, its feature It is:
Step 1), 2) and 3) in,
The reaction condition that described first time enzymolysis, for the second time enzymolysis and third time is digested is respectively in 50 DEG C of reaction 6h;
The condition of described inactivation is 80 DEG C of processing 10min;
The quality consumption of described cellulase is equivalent to the 0.5% of Guava Leaf position quality;
The quality consumption of described hemicellulase is equivalent to the 0.5% of Guava Leaf position quality;
The quality consumption of described beta-glucosidase is equivalent to the 0.5% of Guava Leaf position quality.
9. a kind of Guava Leaf product rich in soluble polyphenol and flavone aglycone, it is characterised in that:By claim 1~ Preparation method described in 8 any one is obtained.
10. the Guava Leaf product rich in soluble polyphenol and flavone aglycone described in claim 9 in field of food and/or Application in field of health care products.
CN201710383696.4A 2017-05-26 2017-05-26 Guava leaf rich in soluble polyphenol and flavonoid aglycone, preparation method and application Active CN107252093B (en)

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WO2018214458A1 (en) * 2017-05-26 2018-11-29 华南理工大学 Guava leaf rich in soluble polyphenols and flavonoid aglycones, preparation method therefor, and application thereof
CN108852929A (en) * 2018-07-10 2018-11-23 珀莱雅化妆品股份有限公司 A kind of preparation method with the guava leaf extract of antiallergic
CN108852929B (en) * 2018-07-10 2021-05-04 珀莱雅化妆品股份有限公司 Preparation method of guava leaf extract with anti-allergy effect
CN109007834A (en) * 2018-08-31 2018-12-18 华南理工大学 A kind of Fructus psidii guajavae immaturus leaf extract cyclodextrin inclusion compound and the preparation method and application thereof
CN110755459A (en) * 2019-11-22 2020-02-07 广西南亚热带农业科学研究所 Extraction process of annona squamosa leaf polyphenol
CN113481242A (en) * 2021-07-19 2021-10-08 福建省农业科学院亚热带农业研究所(福建省农业科学院蔗麻研究中心) Method for improving biological activity of plant polyphenol through microbial transformation
CN118576628A (en) * 2024-07-29 2024-09-03 四川淇力康生物科技有限公司 Method for extracting total flavonoids from sea buckthorn leaves

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