CN107238593A - For diquat dibromide Raman enhanced spectrum method and its agent prescription in fruits and vegetables - Google Patents
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Abstract
One kind is used for diquat dibromide Raman enhanced spectrum method and its agent prescription in fruits and vegetables, it is characterised in that be used as surface enhanced reagent and Cl containing finite concentration using silver colloid aqueous solution A‑Aqueous solution B for auxiliary reagent to strengthen the raman spectral signal of diquat dibromide, with wave number 1593cm‑1Neighbouring Raman spectrum signal carries out diquat dibromide test.For fruits and vegetables, using deionized water as extracts reagent, directly tested after mixing, centrifuging.The inventive method can carry out the qualitative detection primary dcreening operation of diquat dibromide in food sample；Also diquat dibromide in food sample can quantitatively be detected, easy to operate, quick, reagent, consumables cost are cheap, it is easy to penetration and promotion using external standard method according to diquat dibromide raman spectral signal intensity and the linear relationship of its content.
Description
Technical field
The present invention is on a kind of Fast Detection Technique of pesticide residue, more particularly to the Raman spectroscopy of diquat dibromide field quick detection, agent prescription and application method in fruits and vegetables.
Background technology
Food security be one on national economy " project supported by commen people ", be directly connected to the healthy and life security of broad masses of the people.And, food safety affair involves wide, especially in agricultural production after applying pesticides, a part of agricultural chemicals is directly or indirectly residued in cereal, vegetables, fruit, livestock products, aquatic products and soil and water body, and increasingly severe threat is constituted to human health safety or even living environment.Therefore, it is possible to safety, fast and effectively detect the problem of residues of pesticides technologies of the agricultural product such as gourd, fruit and vegetable is paid close attention to jointly as researcher.
Diquat dibromide, sterling is colourless to pale yellow crystals, also known as sharp agriculture, conductibility is generally used for tag steriland herbicide, can be rapidly by green plants tissue resorption, lost activity quickly after being contacted with soil, for the weeding such as before crop field, orchard, bare place, harvesting, the cauline leaf for being also used as potato and pachyrhizus urges withered.Toxicological information shows that diquat dibromide belongs to medium poison class herbicide, its dibromide is about 6~12g to the lethal dose of people, and diquat dibromide is degraded in intestines and stomach by bacterial action, therefore it absorbs few through intestines and stomach, with blood distribution to systemic organs, tissue after absorption, wherein higher with liver, kidney concentration.The toxicity of its metabolite is low compared with its original shape, and enteric microorganism plays a major role to metabolism.Mainly by kidney excretion, also there is a great deal of to pass through bile excretion.Therefore, the residues of pesticides contained in the gourd, fruit and vegetable for using diquat dibromide can cause serious harm to human body.The method of conventional detection diquat dibromide has gas chromatography, liquid chromatography, capillary electrophoresis, square wave voltammetry and these methods and mass spectrographic GC-MS etc. at present.Gas Chromatography-mass Spectrometry and liquid chromatography/mass spectrometry method are the standard methods that our countries are recommended, and conventional chromatography is required for cumbersome time-consuming sample pretreatment process, and analysis time is long.
SERS (SERS) generally occurs in precious metal surfaces such as coarse gold, silver.SERS Electromagnetic enhancement mechanism thinks, when incident laser radiation is to precious metal surface, and surface plasma, which is known from experience, to be excited and cause the enhancing of local electric field, finally causes the very big enhancing of Raman scattering.Therefore, when having the substrate surface of SERS activity when Molecular Adsorption or closely, the Raman signal of molecule can greatly be strengthened.SERS detection techniques are due to having the advantages that sensitivity is high, detection speed is fast, test limit is extremely low and can realize that nondestructive analysis has evolved into a kind of powerful for being widely used in the fields such as Surface Science, analysis science and life science.
This paper presents a kind of method of utilization SERS technology for detection diquat dibromide content, this method operating process is simple, and detection speed is fast, and minimum detection limit can reach 10- 8Mol/L, is expected to develop into a kind of common method for being applied to diquat dibromide and other similar Detecting Pesticides.
The content of the invention
It is an object of the invention to provide the quick determination method that diquat dibromide is remained in a kind of fruits and vegetables, it is used for quickly detecting using extractive technique and SERS to remaining diquat dibromide in fruits and vegetables, it is with low cost, can field application.
To adapt to the demand, the present invention provides simple fruits and vegetables (such as strawberry, Chinese cabbage) pre-treating method, and there is provided detection reagent formula and preparation method thereof, there is provided the qualitative and quantitative analysis method of diquat dibromide field quick detection.Its step is as follows：
1. extract：Sample extracts reagent is used as with deionized water, vegetables or fruit are broken into juice shape, accurately weigh sample 3.0g, 3mL deionized waters rotation nest is added to mix, 5~10min is centrifuged under 8000~10000rpm in centrifuge, the supernatant liquor after centrifugation is drawn, with 0.22 μm of membrane filtration, takes filtrate to be measured.
2. detection：Filtrate is added in advance plus in good a certain amount of reagent A and B detection cell, is vortexed or shake is uniform, be placed in portable Raman Kuai Jian instrument sensing chamber and carry out Raman spectroscopy scans, gather in Raman spectrogram, Raman spectrogram such as in 1593cm-1(wave number error<10cm-1) there is characteristic peak at place, then judgement sample with the addition of diquat dibromide, on the contrary then judge that sample does not add diquat dibromide.The Raman enhanced spectrum method of the present invention, the water is redistilled water or deionized water.
3. reagent：A is silver colloid aqueous solution, is prepared according to known methods；Reagent B is that can condense A containing Cl-Dielectric, Cl-Concentration range is 0.1M~1M；PH is 3-14.The consumption of the reagent is as follows：(1) reagent A：100-300μL；(2) reagent B：100-300μL；(3) sample filtrate：100-500μL.
4. instrument：The LASER Light Source launch wavelength that portable Raman of the present invention examines instrument soon is 785nm.At present, China Inst. of Quarantine Inspection Sciences, (Beijing) Science and Technology Ltd., Bi Da Imtech are ground by Zhong Jian states can provide the Portable Raman spectrometer for meeting test request.
During using the present invention, the data acquisition in later stage and interpretation of result step are as follows：
(1) detected that laser energy parameter selection 300mw, time of integration 10s adjust optimal Raman peak intensity with Portable Raman spectrometer.Apparatus, which has powerful connections, to be deducted the data processing software of function and carries out data processing, the flat Raman collection of illustrative plates of baseline is obtained, because wave number is in 200-300cm-1With 2000-2200cm-1Between spectrogram without difference very little between obvious characteristic peak and spectrogram, so selection 300-2100cm-1Between as this experiment investigation scope.
(2) it can be seen from the gained diquat dibromide aqueous solution strengthens Raman collection of illustrative plates, diquat dibromide is in 1081cm-1、1202cm-1、1248cm-1、1335cm-1、1395cm-1、1541cm-1、1593cm-1(the equal ＜ 10cm of wave number error-1) etc. have Raman strengthen peak, such as occur in that Raman peaks in these places, then can determine the presence of diquat dibromide.In these enhancing peaks, 1593cm-1(wave number error<10cm-1) place enhancing peak intensity it is larger, when diquat dibromide concentration is relatively low, still more substantially and peak and diquat dibromide concentration have more clear and definite linear relationship at this, therefore the peak is the characteristic peak of diquat dibromide at this peak.The negative fruits and vegetables sample of contrast, positive fruits and vegetables sample even up the Raman spectrogram after baseline, such as 1593cm-1(wave number error<10cm-1) place occurs in that obvious Raman peaks, and signal to noise ratio >=3, then is tentatively judged as with the addition of the positive of diquat dibromide, such as 1593cm-1(wave number error<10cm-1) place do not have obvious peak, then is tentatively judged as not adding the negative sample of diquat dibromide.Therefore, 1593cm-1(wave number error<10cm-1) characteristic peak at place is to detect the important evidence of diquat dibromide whether is with the addition of in food, and just mesh analysis can be carried out to fruits and vegetables sample diquat dibromide by this feature peak.
(4) quantitative analysis：According to 1593cm-1(wave number error<10cm-1) relative signal intensity at characteristic frequency corresponding to diquat dibromide and the linear relationship of its concentration do standard curve, and quantitative analysis can be carried out to diquat dibromide in fruits and vegetables with the standard curve.
The SERS method of the present invention, sample pre-treatments are simple, without crossing column purification process, directly use deionized water mechanical shaking extraction, and experiment consumables cost is cheap.Pass through Raman spectrum Figure 159 3cm-1(wave number error<10cm-1) whether there is diquat dibromide characteristic peak, judge whether add diquat dibromide in fruits and vegetables.Its fruit detection limit, the range of linearity and rate of recovery are shown in Table 1, and vegetables detection limit, the range of linearity and the rate of recovery are shown in Table 2.This method is easy to operate quick, and linear relationship is good, and analysis result is intuitively visual, and field quick detection can be achieved if using portable Raman detector, quickly can qualitatively and quantitatively be detected to whether with the addition of the diquat dibromide scene of carrying out in fruits and vegetables.
Diquat dibromide measurement result in the fruit of table 1.
| Sample | Diquat dibromide detection limit (μ g/kg) | The range of linearity (μ g/kg) | The rate of recovery |
| Watermelon | 10 | 10-150 | 104.7% (50 μ g/L) |
| Strawberry | 100 | 100-500 | 93.9% (350 μ g/L) |
Diquat dibromide measurement result in the vegetables of table 2.
| Sample | Diquat dibromide detection limit (μ g/kg) | The range of linearity (μ g/kg) | The rate of recovery |
| Chinese cabbage | 3 | 3-80 | 103.0% (50 μ g/kg) |
| Cucumber | 10 | 10-100 | 93.5% (60 μ g/kg) |
| Potato | 200 | 200-1000 | 95.0% (500 μ g/kg) |
Brief description of the drawings
Fig. 1 is the SERS figure of the diquat dibromide standard items aqueous solution；
SERS figure (the A. watermelon positives of Fig. 2 representativeness fruit (watermelon) positives and negative sample；B. watermelon negative sample)；
Fig. 3 representativeness fruit (watermelon) adds the canonical plotting of diquat dibromide
SERS figure (the A. strawberry positives of Fig. 4 representativeness fruit (strawberry) positives and negative sample；B. strawberry negative sample)；
Fig. 5 representativeness fruit (strawberry) adds the canonical plotting of diquat dibromide
SERS figure (the A. Chinese cabbage positives of Fig. 6 representativeness vegetables (Chinese cabbage) positives and negative sample；B. Chinese cabbage negative sample)；
Fig. 7 representativeness vegetables (Chinese cabbage) add the canonical plotting of diquat dibromide
SERS figure (the A. cucumber positives of Fig. 8 representativeness vegetables (cucumber) positives and negative sample；B. cucumber negative sample)；
Fig. 9 represents the canonical plotting that vegetables (cucumber) add diquat dibromide
SERS figure (the A. potato positives of Figure 10 representativeness vegetables (potato) positives and negative sample；B. potato negative sample)；
Figure 11 represents the canonical plotting that vegetables (potato) add diquat dibromide
Embodiment
Embodiment 1 is a kind of to be used for diquat dibromide Raman enhanced spectrum method and its agent prescription in fruits and vegetables, including in detail below the step of：
1. sample preparation：Fruit is broken into juice shape and 3.0g is weighed, 3mL deionized waters is added and mixes, centrifuge 10min under 8000rpm at room temperature, cross 0.22 μm of filter membrane to be measured.
2. sampling：100 μ L reagent As are taken to be added in detection cell；The μ L of the NaCl aqueous solution 100 are taken to be added in advance plus in the detection cell of good reagent A；The μ L of filtrate 300 are taken to be added in advance plus in good reagent A and NaCl detection cell, it is to be measured.
3. detection：Detected using the Portable Raman spectrometer developed by China Inst. of Quarantine Inspection Sciences, LASER Light Source launch wavelength is 785nm, laser energy parameter selection 300mw, time of integration 10s, adjusts optimal Raman peak intensity.Apparatus, which has powerful connections, to be deducted the data processing software of function and carries out data processing, is obtained the flat Raman collection of illustrative plates of baseline, is selected 300-2000cm-1Between as this experiment investigation scope.First mesh analysis, 1593cm is can be seen that by Raman spectrogram-1(wave number error<10cm-1) place do not have obvious peak, then tentatively judges the sample for the fruit without addition diquat dibromide.
Embodiment 2 is a kind of to be used for diquat dibromide Raman enhanced spectrum method and its agent prescription in fruits and vegetables, including in detail below the step of：
1. sample preparation：Vegetables are broken into juice shape and 3.0g is weighed, 3mL deionized waters is added and mixes, centrifuge 15min under 10000rpm at room temperature, cross 0.22 μm of filter membrane to be measured.
2. sampling：100 μ L reagent As are taken to be added in detection cell；The μ L of the NaCl aqueous solution 100 taken are added in advance plus in the detection cell of good reagent A；The μ L of filtrate 300 are taken to be added in advance plus in good reagent A and NaCl detection cell, it is to be measured.
4. detection：Detected that laser energy parameter selection 300mw adjusts optimal Raman peak intensity using the Portable Raman spectrometer developed by China Inst. of Quarantine Inspection Sciences.Apparatus, which has powerful connections, to be deducted the data processing software of function and carries out data processing, is obtained the flat Raman collection of illustrative plates of baseline, is selected 300-2000cm-1Between as this experiment investigation scope.First mesh analysis, 1593cm is can be seen that by Raman spectrogram-1(wave number error<10cm-1) place do not have obvious peak, then tentatively judges the sample for without addition diquat dibromide.
Claims (6)
1. for diquat dibromide Raman enhanced spectrum method and its agent prescription in fruits and vegetables, it is characterized in that, draw sample extract solution or sample after pre-treatment, it is placed in the detection cell for adding good a certain amount of surface enhanced reagent A and auxiliary reagent B in advance, it is vortexed or shake is uniform, with Raman spectrometer in 200~2200cm-1In the range of carry out Raman spectroscopy scans, with wave number 1593cm-1Neighbouring Raman spectral peaks are the Raman spectrum signal of diquat dibromide, and according to the raman spectral signal intensity of diquat dibromide and the linear relationship of diquat dibromide content, the scene of carrying out of diquat dibromide in sample is quickly qualitatively and quantitatively detected.
2. according to claim 1 be used for diquat dibromide Raman enhanced spectrum method and its agent prescription in fruits and vegetables, it is characterised in that including following operating procedure：
(1) sample pre-treatment：It is accurate to weigh the fruits and vegetables sample 3g for being broken into juice shape, 3ml deionized waters are added, mixes, in centrifuging 5~10min under 8000~15000rpm of room temperature, draws the supernatant liquor after centrifugation, cross 0.22 μm of membrane filtration, it is to be measured；
(2) detect：Careful test solution of drawing is vortexed in advance plus in good a certain amount of surface enhanced reagent A and auxiliary reagent B detection cell or shake is uniform, carry out Raman spectroscopy scans detection；Using external standard method and select 1593cm-1Wave number corresponding to neighbouring highest Raman spectral peaks is the characteristic waves that diquat dibromide is detected, according to the raman spectral signal intensity of diquat dibromide and the linear relationship of diquat dibromide content, carries out the fast quantification and qualitative detection of diquat dibromide in sample.
3. according to claim 1 be used for diquat dibromide Raman enhanced spectrum method and its agent prescription in fruits and vegetables, it is characterised in that including following agent prescription and application method：
With deionized water as sample extracts reagent, surface enhanced reagent A is silver colloid aqueous solution (preparing according to known methods), and auxiliary reagent B is that can condense A containing Cl-Dielectric；Described dielectric, it is characterised in that：Cl-Concentration range be 0.1M~1M；PH is 3-14；The consumption of the surface enhanced reagent A, auxiliary reagent B and test solution is as follows：Reagent A：80-300μL；Reagent B：80-300μL；Test solution consumption is 50-500 μ L.
4. according to claim 1 be used for diquat dibromide Raman enhanced spectrum method and its agent prescription in fruits and vegetables, it is characterized in that, used Raman spectrum detector can be hand-held, portable or desk-top instrument, should possess the LASER Light Source that launch wavelength is 785nm, its wave number error<10cm-1, the units such as (Beijing) Science and Technology Ltd., Bi Da Imtech are ground by current China Inst. of Quarantine Inspection Sciences, Zhong Jian states can provide the Portable Raman spectrometer for meeting test request.
5. according to claim 1 be used for diquat dibromide Raman enhanced spectrum method and its agent prescription in fruits and vegetables, it is characterised in that its operating procedure includes：
(1) sample：80-300 μ L surface enhanced reagent As are taken in detection cell, 80-300 μ L auxiliary reagent B are added, the careful μ L of homogeneous test solution 100, the 200 μ L or 400uL drawn after centrifugation are in detection cell；
(2) instrument parameter is set：Preferred laser energy parameter and time of integration parameter obtain the optimal Raman peak intensity of Raman spectrometer, and preferably continuous scanning optical spectrum number of times obtains most preferably average scanning optical spectrum；
(3) scan：With Raman spectrum detector in 200~2200cm-1In the range of sweep test sample.
6. according to claim 1 be used for diquat dibromide Raman enhanced spectrum method and its agent prescription in fruits and vegetables, its data acquisition and interpretation of result step include：
(1) it is internal standard benchmark to select the intensity corresponding to the Raman signal peak under a certain characteristic waves, carries out spectrogram normalization；
(2) apparatus, which has powerful connections, deducts the data processing software of function and carries out data processing, obtains the flat Raman collection of illustrative plates of baseline；
(3) qualitative analysis：Gather the Raman spectrogram of food sample, the corresponding characteristic peak 1593cm of such as diquat dibromide-1Place occurs in that obvious Raman peaks, and signal to noise ratio >=3, then is tentatively judged as with the addition of the positive of diquat dibromide, such as characteristic waves 1593cm-1There is no obvious peak, be then tentatively judged as not adding the negative sample of diquat dibromide；
(4) quantitative analysis：According to characteristic waves 1593cm-1Relative signal intensity and the linear relationship of its concentration at characteristic waves corresponding to diquat dibromide do standard curve, and quantitative analysis can be carried out to diquat dibromide in food with the standard curve.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109211872A (en) * | 2018-09-04 | 2019-01-15 | 广东朗源生物科技有限公司 | A kind of full-automatic pesticide residue detection method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104142321A (en) * | 2014-07-24 | 2014-11-12 | 江西农业大学 | Method for fast detecting surface enhancing Raman spectrums of pesticide residues in tea leaves |
| CN104597024A (en) * | 2014-12-01 | 2015-05-06 | 中检国研(北京)科技有限公司 | Raman spectrometry used for rapidly detecting paraquat in fruit and vegetable on site |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104142321A (en) * | 2014-07-24 | 2014-11-12 | 江西农业大学 | Method for fast detecting surface enhancing Raman spectrums of pesticide residues in tea leaves |
| CN104597024A (en) * | 2014-12-01 | 2015-05-06 | 中检国研(北京)科技有限公司 | Raman spectrometry used for rapidly detecting paraquat in fruit and vegetable on site |
Non-Patent Citations (1)
| Title |
|---|
| 刘培培等: "基于表面增强拉曼散射的敌草快检测方法", 《高等学校化学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109211872A (en) * | 2018-09-04 | 2019-01-15 | 广东朗源生物科技有限公司 | A kind of full-automatic pesticide residue detection method |
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