CN107238586A - A kind of biological method for sensing for detecting glutathione - Google Patents
A kind of biological method for sensing for detecting glutathione Download PDFInfo
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- CN107238586A CN107238586A CN201710281494.9A CN201710281494A CN107238586A CN 107238586 A CN107238586 A CN 107238586A CN 201710281494 A CN201710281494 A CN 201710281494A CN 107238586 A CN107238586 A CN 107238586A
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- manganese dioxide
- phenylenediamine
- nano material
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- glutathione
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Abstract
The present invention relates to a kind of biological method for sensing for detecting glutathione.The present invention is using nano material of manganese dioxide as oxidant and recognition component, when without glutathione (GSH), nano material of manganese dioxide catalysis oxidation o-phenylenediamine, which produces oxidation o-phenylenediamine product, is used for fluoroscopic examination, in the presence of GSH, GSH degraded nano material of manganese dioxide, suppress oxidability of the manganese dioxide to o-phenylenediamine, weaken fluorescence signal.The detection to GSH is realized in change by comparing fluorescent value.Relative to prior art, present invention operation is more easy, and sensitivity is high, simple, with low cost, can specific detection GSH；The detection method of the present invention can be efficiently applied to the detection of GSH in biological fluid.
Description
(1) technical field
The present invention relates to a kind of biological method for sensing for detecting glutathione, belong to bioanalysis detection technique neck
Domain.
(2) background technology
The tripeptides that glutathione (GSH) is made up of glutamic acid, cysteine and glycine, is that content is most in living cells
Many non protein thiols, are resistant to the first line of defence of toxin.It keep cell balance, cell signalling, gene regulation,
Play an important roll in terms of removing toxic substances.Generally, the unbalance of GSH can cause a variety of diseases to occur, such as hepatopathy, AIDS, diabetes,
Cancer etc..So, develop sensitive efficient GSH detection methods significant.
At present, the detection for GSH has electrochemical process, high performance liquid chromatography, colorimetric method, fluorescence method etc..Wherein, fluorescence
Method has high sensitivity, non-destructive, simple operation and other advantages.Recently, a variety of organic probes are exploited for GSH detections,
Such as utilize Michael addition reaction, disulfide bonds, sulfydryl nucleophilic substitution etc..Although obtaining good result, big portion
The synthesis of point organic probes and purification step are more, and cost is higher, limit its application.Also there is some fluorescent nano materials such as quantum
Point, gold nanoclusters, fluorescence carbon nanomaterial, upper conversion nano particle are exploited for GSH detections, but have some shortcomings to deposit
Heavy metal cadmium is being used during such as quantum dot synthesis, golden cluster or fluorescence carbon nanomaterial uses mercury ion and comes quenching fluorescence, upper conversion
Nano particle synthesis is complicated and cost is high.So, develop simple to operate, high sensitivity, the fluoroscopic examination GSH methods of high selectivity
Still there is higher scientific value and practical value.
(3) content of the invention
Present invention aims at the deficiencies in the prior art are improved, there is provided a kind of simple to operate, sensitive high, selectivity is high
GSH fluorescence detection methods.
The technical solution adopted by the present invention is：
A kind of biological method for sensing for detecting glutathione, methods described includes：
(1) chemical reduction method synthesis nano material of manganese dioxide, prepares o-phenylenediamine solution；
(2) by nano material of manganese dioxide and o-phenylenediamine solution hybrid reaction, fluorescence spectrum survey is carried out to reaction product
It is fixed, obtain fluorescence reference point F0；Reaction temperature is 20~90 DEG C, and the reaction time is 1 minute~1 hour, manganese dioxide nano material
Material concentration is the μ g/mL of 1 μ g/mL~500, and o-phenylenediamine concentration is 0.1mM~100mM, nano material of manganese dioxide：O-phenylenediamine
The ratio between quality consumption is 1：1~50；
(3) gradient concentration glutathione standard liquid and nano material of manganese dioxide are reacted, by solution after reaction
With o-phenylenediamine solution mixing coreaction, nano material of manganese dioxide and the same step of o-phenylenediamine solution consumption (2) determine reaction
Product fluorescent value F, using glutathione concentrations as abscissa, with F0- F values are ordinate, obtain various concentrations glutathione response
Standard curve；
(4) sample solution containing glutathione to be measured reacts with nano material of manganese dioxide, by solution after reaction and adjacent benzene two
Amine aqueous solution mixing coreaction, nano material of manganese dioxide and the same step of o-phenylenediamine solution consumption (2), determine reaction product fluorescence
Value, reference standard curve obtains sample GSH-PX activity concentration value.
The present invention is using nano material of manganese dioxide as oxidant and recognition component, when without GSH, nano material of manganese dioxide
Catalysis oxidation o-phenylenediamine, which produces oxidation o-phenylenediamine product, is used for fluoroscopic examination, in the presence of GSH, GSH degraded manganese dioxide
Nano material, suppresses oxidability of the manganese dioxide to o-phenylenediamine, weakens fluorescence signal.It is real by the change for comparing fluorescent value
Now to GSH detection.The inventive principle schematic diagram is as shown in Figure 1.
It is preferred that, step (1) the nano material of manganese dioxide synthetic method is as follows：Liquor potassic permanganate adds 2- (N-
Morpholine) in ethanesulfonic acid buffer, ultrasonic reaction obtains brown product, centrifuge washing 3~5 times, and being scattered in again in secondary water makes
It is 1mg/mL to obtain concentration；Wherein, potassium permanganate concentration is 0.1~100mM, and 2- (N- morpholines) ethanesulfonic acid buffer concentration is 1mM
~1M, pH value are 5.0~7.4, and ultrasonic time is 10 minutes~2 hours.
Step (1) the o-phenylenediamine solution compound method is as follows：108mg o-phenylenediamine solids are weighed, 0.5mL second is added
9.5mL secondary waters, which are added, after alcohol dissolving is configured to 100mM o-phenylenediamine solutions.
Step (3) glutathione solution concentration is 0~100 μM, and the reaction time is 1 minute~1 hour.
Step (4) GSH-PX activity sample solution can be the aqueous solution, cushioning liquid, blood serum sample or cell pyrolysis liquid sample
Deng.If testing sample concentration is higher, detected again after can diluting.
The beneficial effects are mainly as follows：Relative to prior art, the present invention operates more easy, sensitivity
It is high, simple, with low cost, can specific detection GSH；The detection method of the present invention can be efficiently applied in biological fluid
GSH detection.
(4) illustrate
Fig. 1 is the principle schematic for detecting glutathione of the invention.
The manganese dioxide nano-plates phenogram that Fig. 2 is prepared for the present invention；(A) liquor potassic permanganate and generation manganese dioxide
Absorb figure；(B) transmission electron microscope picture of manganese dioxide nano-plates；(C) Raman spectrogram of manganese dioxide nano-plates；(D) titanium dioxide
The x-ray photoelectron energy spectrum diagram (Mn2p) of manganese nanometer sheet.
Fig. 3 is the glutathione fluorescence spectra and linear graph that manganese dioxide nano-plates are used to detect various concentrations, and (A) is glimmering
Photoresponse figure；(B) corresponding fluorescence and concentration-response linear correlation figure.Wherein, F0Not add fluorescent value during glutathione,
F is fluorescent value after addition various concentrations glutathione.
Fig. 4 is the selective lab diagram that manganese dioxide nano-plates detect glutathione, and selected chaff interference is respectively：Phosphoric acid
Salt buffer (PBS), Klorvess Liquid (KCl), sodium chloride (NaCl), magnesium chloride (MgCl2), concentrations above is 100mM；Grape
Sugared (glucose), glycine (Gly), serine (Ser), lysine (Lys), aspartic acid (Asp), concentrations above is 10mM；
Bovine serum albumin(BSA) (BSA), human serum albumins (HSA), glucose oxidase (GOx), concentrations above is 10mg/mL.
Fig. 5 is the fluorescence spectra that manganese dioxide nano-plates detect cell pyrolysis liquid GSH-PX activity.(A) different numbers are detected
Glutathione in mesh cell pyrolysis liquid；(B) after cell pyrolysis liquid is pre-processed through NEM, fluorescence response change.
Fig. 6 is the fluorescence spectra that manganese dioxide nano-plates detect serum GSH-PX activity.
(5) embodiment
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This：
Embodiment 1：
The synthesis of nano material of manganese dioxide：1mL liquor potassic permanganates (10mM) and 2.5mL MES buffer solutions (0.1M, pH
6.0) after mixing, 6.5mL secondary waters are added；Afterwards, ultrasonic reaction 30 minutes in ultrasonic cleaning machine are placed in, to the cotton-shaped production of brown
Thing is formed.Brown product 8000 rpm is centrifuged and washed 3 times for 10 minutes after reacting, to remove unreacted ion.Most
Afterwards, by obtained manganese dioxide nano-plates ultrasonic disperse in secondary water, concentration is 1mg/mL.Fig. 2 proves to successfully synthesize two
Manganese material is aoxidized, wherein, (A) figure is the absorption comparison diagram after liquor potassic permanganate and generation manganese dioxide；(B) figure is after synthesizing
Transmission electron microscope picture, it was demonstrated that synthesized the manganese dioxide nano-plates of nano-scale；(C) figure is the Raman of manganese dioxide nano-plates
Spectrogram；(D) figure is the x-ray photoelectron energy spectrum diagram (XPS) of manganese dioxide nano-plates.Result above proves manganese dioxide nano
The successful synthesis of piece.
Embodiment 2：
Fluoroscopic examination glutathione：2.5 μ L manganese dioxide nano-plates (1mg/mL) and 200 μ L various concentrations glutathione
Solution (0,10,20,30,40 μM) and room temperature reaction 5 minutes；Afterwards, o-phenylenediamine (OPDA) solution for adding 5 μ L6.8mM is mixed
It is even, after being reacted 10 minutes in 50 DEG C of baking ovens or water-bath.Room temperature is cooled to, 420nm wavelength excites lower test 568nm's glimmering
Optical emission spectroscopy.When doing selectivity experiment, disturbance thing and 2.5 μ L manganese dioxide nano-plates (1mg/mL) are reacted at room temperature
5 minutes；Afterwards, o-phenylenediamine (OPDA) solution for adding 5 μ L 6.8mM is mixed, and 10 are reacted in 50 DEG C of baking ovens or water-bath
After minute.It is cooled to after room temperature, re-test fluorescence spectra.The fluorescence response of glutathione is as shown in Figure 3.This method is to paddy Guang
The selectivity of sweet peptide detection, as shown in figure 4, in addition to glutathione, other non-targeted molecules suppress manganese dioxide oxygen without obvious
Change the ability of o-phenylenediamine, it was demonstrated that detection method of the invention has good selectivity to glutathione.
Embodiment 3：
The detection of cell lysate solution GSH-PX activity：HeLa cell culture is containing 10% hyclone, penicillin
Contain 5% CO2gas incubator in 37 DEG C of high humility in the culture mediums of RPMI 1640 of (100U/mL) and streptomysin (100 μ g/mL)
In.After the HeLa cells grown are centrifuged, washed with cold PBS after 3 times, the concentration of cell is calculated using cell counter.
Cell suspending liquid is dispersed in cold PBS so that in every 100 μ L solution containing 500,5000,10000,20000,30000,
The different number of cells such as 40000, ultrasound 5 minutes, 10000 rpms under 4 DEG C of condition of ice bath is placed in by cell suspending liquid
After 5 points of centrifugation, supernatant is taken to be used to test.The manganese dioxide nano-plates (1mg/mL) that supernatant is diluted to 200 μ L and 2.5 μ L are mixed
After closing reaction 5 minutes, o-phenylenediamine (OPDA) solution for adding 5 μ L6.8mM is mixed, and is reacted in 50 DEG C of baking ovens or water-bath
After 10 minutes.It is cooled to after room temperature, 420 nano wave lengths excite the fluorescence emission spectrum of 568 nanometers of lower test.In addition, having one
Group cell pyrolysis liquid is first reacted to close mercaptan material with 0.1mM ethyl maleimides (N-ethylmaleimide, NEM),
For proving that fluorescence signal change comes from the glutathione in cell.As a result it is as shown in Figure 5.Prove that this method can be used for
Glutathione detection in cell pyrolysis liquid.
Embodiment 4：
The detection of serum GSH-PX activity：After the centrifugation 10 minutes of prior to 10000 rpms of blood serum sample, take supernatant dilute
Release 50 times.Added in serum dilution after various concentrations glutathione, by 200 μ L dilute samples containing glutathione and 2.5 μ L
Manganese dioxide nano-plates (1mg/mL) hybrid reaction is after 5 minutes, and the o-phenylenediamine solution for adding 5 μ L 6.8mM is mixed, in 50 DEG C
After being reacted 10 minutes in baking oven or water-bath.It is cooled to after room temperature, 420nm wavelength excites the fluorescence at lower test 568nm to send out
Penetrate spectrum.As shown in fig. 6, as glutathione concentrations increase, fluorescence signal is gradually reduced, it was demonstrated that this method can be used in serum
The detection of glutathione.
Claims (5)
1. a kind of biological method for sensing for detecting glutathione, methods described includes：
(1) chemical reduction method synthesis nano material of manganese dioxide, prepares o-phenylenediamine solution；
(2) by nano material of manganese dioxide and o-phenylenediamine solution hybrid reaction, fluorescence spectrometry is carried out to reaction product, obtained
Obtain fluorescence reference point F0；Reaction temperature is 20~90 DEG C, and the reaction time is 1 minute~1 hour, nano material of manganese dioxide concentration
For the μ g/mL of 1 μ g/mL~500, o-phenylenediamine concentration is 0.1mM~100mM, nano material of manganese dioxide：O-phenylenediamine quality is used
The ratio between amount is 1：1~50；
(3) gradient concentration glutathione standard liquid and nano material of manganese dioxide are reacted, by solution after reaction and neighbour
Phenylenediamine solution mixing coreaction, nano material of manganese dioxide and the same step of o-phenylenediamine solution consumption (2), determine reaction product
Fluorescent value F, using glutathione concentrations as abscissa, with F0- F values are ordinate, obtain various concentrations glutathione response standard
Curve；
(4) sample solution containing glutathione to be measured reacts with nano material of manganese dioxide, and solution after reaction and o-phenylenediamine is molten
Liquid mixing coreaction, nano material of manganese dioxide and the same step of o-phenylenediamine solution consumption (2), determine reaction product fluorescent value,
Reference standard curve, obtains sample GSH-PX activity concentration value.
2. the method as described in claim 1, it is characterised in that step (1) the nano material of manganese dioxide synthetic method is such as
Under：Liquor potassic permanganate is added in 2- (N- morpholines) ethanesulfonic acid buffer, and ultrasonic reaction obtains brown product, centrifuge washing 3~5
It is secondary, it is scattered in again in secondary water and causes concentration to be 1mg/mL；Wherein, potassium permanganate concentration is 0.1~100mM, 2- (N-
Quinoline) ethanesulfonic acid buffer concentration is that 1mM~1M, pH value are 5.0~7.4, ultrasonic time is 10 minutes~2 hours.
3. the method as described in claim 1, it is characterised in that step (1) the o-phenylenediamine solution compound method is as follows：Claim
Take 108mg o-phenylenediamine solids, add and 9.5mL secondary waters are added after the dissolving of 0.5mL ethanol to be configured to 100mM o-phenylenediamines molten
Liquid.
4. the method as described in claim 1, it is characterised in that step (3) glutathione solution concentration is 0~100 μM, reaction
Time is 1 minute~1 hour.
5. the method as described in claim 1, it is characterised in that the method as described in claim 1, it is characterised in that gluathione
Peptide sample solution is the aqueous solution, cushioning liquid, blood serum sample or cell pyrolysis liquid sample.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108444963A (en) * | 2018-03-13 | 2018-08-24 | 中国人民解放军国防科技大学 | Method for detecting glutathione and application of gold-sulfur compound |
| CN108982440A (en) * | 2018-06-10 | 2018-12-11 | 福建医科大学 | The upper building for converting faint photodetector and its detection for glutathione |
| CN109852383A (en) * | 2018-12-26 | 2019-06-07 | 中国药科大学 | The fluorescence probe and its preparation method and application for rapidly and efficiently responding glutathione based on fullerene |
| CN110736724A (en) * | 2019-09-24 | 2020-01-31 | 厦门大学 | Detection method of reduced glutathione |
| CN110982521A (en) * | 2019-12-31 | 2020-04-10 | 山西医科大学 | Graphite phase carbon nitride quantum dot composite and synthetic method and biological application thereof |
| CN111077124A (en) * | 2019-12-30 | 2020-04-28 | 中国科学院烟台海岸带研究所 | Red-yellow-blue three-fluorescence emission sensor and preparation and application thereof |
| CN111346676A (en) * | 2020-03-09 | 2020-06-30 | 吉林大学 | Iron-substituted tungstophosphoric acid polydopamine nano mimic enzyme and preparation method and application thereof |
| CN111982873A (en) * | 2020-08-14 | 2020-11-24 | 福建医科大学 | Based on Au3+Mark-free colorimetric method for regulated o-phenylenediamine autocatalytic oxidation and application thereof |
| CN112362646A (en) * | 2020-10-27 | 2021-02-12 | 华南理工大学 | Glutathione sensor based on nanoenzyme, and preparation method and application thereof |
| CN112540064A (en) * | 2020-11-04 | 2021-03-23 | 江苏大学 | Method for detecting acrylamide content in baked food based on up-conversion fluorescent nano system |
| CN113461063A (en) * | 2021-06-29 | 2021-10-01 | 哈尔滨工业大学 | Preparation method and application of manganese dioxide nanoenzyme |
| CN115057472A (en) * | 2022-06-21 | 2022-09-16 | 中国医学科学院基础医学研究所 | Novel fluorescence sensing system and application thereof in PTP-1B detection |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108444963A (en) * | 2018-03-13 | 2018-08-24 | 中国人民解放军国防科技大学 | Method for detecting glutathione and application of gold-sulfur compound |
| CN108982440A (en) * | 2018-06-10 | 2018-12-11 | 福建医科大学 | The upper building for converting faint photodetector and its detection for glutathione |
| CN109852383A (en) * | 2018-12-26 | 2019-06-07 | 中国药科大学 | The fluorescence probe and its preparation method and application for rapidly and efficiently responding glutathione based on fullerene |
| CN110736724A (en) * | 2019-09-24 | 2020-01-31 | 厦门大学 | Detection method of reduced glutathione |
| CN111077124B (en) * | 2019-12-30 | 2022-08-02 | 中国科学院烟台海岸带研究所 | Red-yellow-blue three-fluorescence emission sensor and preparation and application thereof |
| CN111077124A (en) * | 2019-12-30 | 2020-04-28 | 中国科学院烟台海岸带研究所 | Red-yellow-blue three-fluorescence emission sensor and preparation and application thereof |
| CN110982521A (en) * | 2019-12-31 | 2020-04-10 | 山西医科大学 | Graphite phase carbon nitride quantum dot composite and synthetic method and biological application thereof |
| CN110982521B (en) * | 2019-12-31 | 2023-03-03 | 山西医科大学 | Graphite phase carbon nitride quantum dot composite and synthetic method and biological application thereof |
| CN111346676A (en) * | 2020-03-09 | 2020-06-30 | 吉林大学 | Iron-substituted tungstophosphoric acid polydopamine nano mimic enzyme and preparation method and application thereof |
| CN111346676B (en) * | 2020-03-09 | 2023-04-07 | 吉林大学 | Iron-substituted tungstophosphoric acid polydopamine nano mimic enzyme as well as preparation method and application thereof |
| CN111982873A (en) * | 2020-08-14 | 2020-11-24 | 福建医科大学 | Based on Au3+Mark-free colorimetric method for regulated o-phenylenediamine autocatalytic oxidation and application thereof |
| CN111982873B (en) * | 2020-08-14 | 2023-12-01 | 福建医科大学 | Au-based 3+ Label-free colorimetric method for self-catalytic oxidation of regulated o-phenylenediamine and application thereof |
| CN112362646A (en) * | 2020-10-27 | 2021-02-12 | 华南理工大学 | Glutathione sensor based on nanoenzyme, and preparation method and application thereof |
| CN112362646B (en) * | 2020-10-27 | 2022-04-22 | 华南理工大学 | Glutathione sensor based on nanoenzyme, and preparation method and application thereof |
| CN112540064A (en) * | 2020-11-04 | 2021-03-23 | 江苏大学 | Method for detecting acrylamide content in baked food based on up-conversion fluorescent nano system |
| CN113461063A (en) * | 2021-06-29 | 2021-10-01 | 哈尔滨工业大学 | Preparation method and application of manganese dioxide nanoenzyme |
| CN113461063B (en) * | 2021-06-29 | 2022-10-25 | 哈尔滨工业大学 | Preparation method and application of manganese dioxide nanoenzyme |
| CN115057472A (en) * | 2022-06-21 | 2022-09-16 | 中国医学科学院基础医学研究所 | Novel fluorescence sensing system and application thereof in PTP-1B detection |
| CN115057472B (en) * | 2022-06-21 | 2023-10-27 | 中国医学科学院基础医学研究所 | Novel fluorescence sensing system and application thereof in PTP-1B detection |
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Application publication date: 20171010 |
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