CN107235851A - A kind of method of extract and separate amino acid - Google Patents
A kind of method of extract and separate amino acid Download PDFInfo
- Publication number
- CN107235851A CN107235851A CN201710434616.3A CN201710434616A CN107235851A CN 107235851 A CN107235851 A CN 107235851A CN 201710434616 A CN201710434616 A CN 201710434616A CN 107235851 A CN107235851 A CN 107235851A
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- China
- Prior art keywords
- amino acid
- organic solvent
- aqueous solution
- organic
- containing amino
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The present invention relates to chemical field, a kind of method of extract and separate amino acid is specifically disclosed.By the aqueous solution containing amino acid by being mixed with organic solvent, it is concentrated by evaporation the moisture removed in amino acid solution, so that amino acid molecular preferably enters organic phase, the organic phase product containing amino acid is obtained and inorganic salts and the less entrance organic phase of other organic impurities, so as to realize amino acid and the separation of the other impurities in the aqueous solution.It can be obtained into the amino acid in organic phase by a variety of traditional methods known to industry technical staff to more pure amino acid, amino-acid salt and/or other amino acid products.The method of the invention extract and separate amino acid, process route is short, is easy to operation, and gained amino acid purity is high.Amino acid products high income, environmental pollution are small, it is to avoid complicated operating process in nontraditional amino acid production technology.
Description
Technical field
The present invention relates to chemical field, specifically, it is related to the method for extract and separate amino acid.
Background technology
The extracting process of current amino acid is mainly conventional liquid-liquid extraction method, is added in liquid mixture and its not phase
The selected solvent of miscible (or slightly miscible), separation is reached using its component different solubilities in a solvent or mesh is extracted
's.Amino acid determines the complexity that organic phase is transferred to by extraction component in aqueous phase and the distribution coefficient in organic phase.
Therefore, for current amino acids production technique is cumbersome, product yield is low and high energy consumption situation, need badly and propose one
Plant more economic, the effective method for preparing amino acid.
The content of the invention
In order to solve problems of the prior art, it is an object of the invention to provide a kind of side of extract and separate amino acid
Method.
In order to realize the object of the invention, technical scheme is as follows：
The present invention provides a kind of method of extract and separate amino acid, comprises the following steps：
1) the crude product aqueous solution containing amino acid is mixed with organic solvent, concentration is evaporated to mixed liquor and goes water removal
Point, obtain the organic solvent containing amino acid；
2) extraction step 1) gained in amino acid.
The extracting method can use conventional method well-known to those skilled in the art or conventional use of extracting method,
Amino acid/the amino-acid salt for entering organic phase is extracted from organic solvent, more pure amino acid/amino acid is obtained
Salt/other amino acid products.
Further, the amino acid includes but is not limited to alanine, methionine, threonine or lysine.
The crude product aqueous solution containing amino acid is：(1) amino acid zymotic fluid obtained by fermentation or enzymatic conversion；
(2) amino acid zymotic fluid treated by other method；(3) aqueous solution containing amino acid obtained by other approach.
The crude product aqueous solution containing amino acid can also be the aqueous solution by pre-concentration.
Further, the organic solvent is selected from the mixture of a kind of following organic compound or a variety of organic compounds：
Esters (such as amyl acetate), ketone (such as 2-HEPTANONE), ethers (such as butyl ether), alcohols (such as octanol), esters (such as ammonia
Base acid tributyl etc.), phosphoric acid ester (such as tributyl phosphate), phosphorus oxygen class (trioctylphosphine oxide (TOPO)), (triisobutyl vulcanizes phosphine sulphur class
Phosphine), amine (such as trioctylamine), amide-type (N.N- dibutyl formamides), other boiling points are more than 100 DEG C at ambient pressure and organising
Compound.
Further, the mixed proportion of the crude product aqueous solution containing amino acid and organic solvent is 1：80~80：1.
Preferably, the mixed proportion of the crude product aqueous solution containing amino acid and organic solvent is 1：50~50：1.
Further, the condition of the evaporation and concentration is is concentrated in vacuo, and temperature is 30~85 DEG C.
Preferably, temperature is 40~75 DEG C.
Further, the step 2) be specially：Organic solvent containing amino acid is mixed with water, the ammonia purified
Base acid solution；Or mix the organic solvent containing amino acid with alkalescence/acid solution, obtain amino acid salt solution.
Organic solvent after amino acid separation can return to step 1) middle recycling.
Preferably, the temperature when organic solvent containing amino acid is mixed with water/alkalescence/acid solution is 10~100
℃。
The present invention relates to raw material or reagent be ordinary commercial products, the operation being related to is unless otherwise specified
This area routine operation.
On the basis of common sense in the field is met, above-mentioned each optimum condition can be mutually combined, obtain specific embodiment party
Formula.
The beneficial effects of the present invention are：
The method of the invention extract and separate amino acid, process route is short, is easy to operation, and gained amino acid purity is high.Ammonia
Base acid product high income, environmental pollution be small, it is to avoid complicated operating process in nontraditional amino acid production technology.
Embodiment
The preferred embodiment of the present invention is described in detail below in conjunction with embodiment.It will be appreciated that following real
Providing merely to play the purpose of explanation for example is applied, is not used to limit the scope of the present invention.The skill of this area
Art personnel can carry out various modifications and replacement in the case of without departing substantially from spirit of the invention and spirit to the present invention.
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material, reagent used etc., unless otherwise specified, are commercially obtained in following embodiments.
Embodiment 1
12% (mass percent) alanine solution 200g is taken, organic solvent butyl ether 400g is put in three-necked flask, in 60
DEG C it is concentrated in vacuo, is concentrated into after being come out there is no condensed water, has obtained the organic solvent containing alanine.
Organic solvent containing alanine is transferred in another three-necked flask, add water with it is organic molten containing alanine
Agent is in 60 DEG C of mixing then by the isolated solution containing alanine of organic solvent.
It is computed, the yield of alanine is 98%.
After testing, isolated alanine purity is 98.5%.
Embodiment 2
Take 15% (mass percent) lysine solution 175g, organic solvent 400g (wherein amyl acetate 150g, butyl ether
60g, amino acid tributyl 190g) it is put in three-necked flask, it is concentrated in vacuo in 70 DEG C, is concentrated into and is come out there is no condensed water
Afterwards, the organic solvent containing lysine has been obtained.
Organic solvent containing lysine is transferred in another three-necked flask, the aqueous solution is added and contains lysine
Organic solvent is in 70 DEG C of mixing then by the isolated solution containing lysine of organic solvent.
It is computed, the yield of lysine is 98%.
After testing, isolated lysine purity is 98.5%.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (8)
1. a kind of method of extract and separate amino acid, it is characterised in that comprise the following steps：
1) the crude product aqueous solution containing amino acid is mixed with organic solvent, being evaporated concentration to mixed liquor removes moisture removal, obtains
To the organic solvent containing amino acid；
2) extraction step 1) gained in amino acid.
2. according to the method described in claim 1, it is characterised in that the amino acid include but is not limited to alanine, methionine,
Threonine or lysine.
3. method according to claim 2, it is characterised in that the crude product aqueous solution containing amino acid is by pre- dense
The aqueous solution of contracting.
4. the method according to any one of claims 1 to 3, it is characterised in that the organic solvent, which is selected from following one kind, to be had
The mixture of machine compound or a variety of organic compounds：
Esters, ketone, ethers, alcohols, esters, phosphoric acid ester, phosphorus oxygen class, phosphine sulphur class, amine, amide-type, other boiling points are normal
Organic compound of the pressure more than 100 DEG C.
5. the method according to any one of claims 1 to 3, it is characterised in that the crude product aqueous solution containing amino acid
Mixed proportion with organic solvent is 1：100~100：1.
6. method according to claim 5, it is characterised in that the condition of the evaporation and concentration is is concentrated in vacuo, material temperature
Spend for 10~90 DEG C.
7. method according to claim 6, it is characterised in that the step 2) be specially：The organic of amino acid will be contained
Solvent is mixed with water, the Freamine Ⅲ purified；Or mix the organic solvent containing amino acid with alkalescence/acid solution
Close, obtain amino acid salt solution.
8. method according to claim 7, it is characterised in that the organic solvent containing amino acid and water/alkalescence/acidity are molten
Temperature when liquid is mixed is 10~100 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710434616.3A CN107235851A (en) | 2017-06-09 | 2017-06-09 | A kind of method of extract and separate amino acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710434616.3A CN107235851A (en) | 2017-06-09 | 2017-06-09 | A kind of method of extract and separate amino acid |
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| Publication Number | Publication Date |
|---|---|
| CN107235851A true CN107235851A (en) | 2017-10-10 |
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ID=59986208
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201710434616.3A Pending CN107235851A (en) | 2017-06-09 | 2017-06-09 | A kind of method of extract and separate amino acid |
Country Status (1)
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2894954A (en) * | 1957-04-25 | 1959-07-14 | Eagle Ottawa Leather Company | Method of selectively extracting amino acids |
| CN85101575A (en) * | 1984-02-24 | 1987-01-10 | 孟山都公司 | The preparation method of liquid 2-hydroxy-4-methylthiobutyric acid |
| CN87104295A (en) * | 1986-06-25 | 1988-03-02 | 罗纳·布朗克化学公司 | Method separating aminoacid |
-
2017
- 2017-06-09 CN CN201710434616.3A patent/CN107235851A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2894954A (en) * | 1957-04-25 | 1959-07-14 | Eagle Ottawa Leather Company | Method of selectively extracting amino acids |
| CN85101575A (en) * | 1984-02-24 | 1987-01-10 | 孟山都公司 | The preparation method of liquid 2-hydroxy-4-methylthiobutyric acid |
| CN87104295A (en) * | 1986-06-25 | 1988-03-02 | 罗纳·布朗克化学公司 | Method separating aminoacid |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171010 |
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| RJ01 | Rejection of invention patent application after publication |