CN107232195A - A kind of method for suppressing aspergillus flavus using 2 nonanones - Google Patents

A kind of method for suppressing aspergillus flavus using 2 nonanones Download PDF

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Publication number
CN107232195A
CN107232195A CN201710404388.5A CN201710404388A CN107232195A CN 107232195 A CN107232195 A CN 107232195A CN 201710404388 A CN201710404388 A CN 201710404388A CN 107232195 A CN107232195 A CN 107232195A
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CN
China
Prior art keywords
methyl
aspergillus flavus
heptyl ketone
heptyl
ketone
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Pending
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CN201710404388.5A
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Chinese (zh)
Inventor
赵月菊
刘阳
宗英
邢福国
王龑
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Institute of Food Science and Technology of CAAS
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Institute of Food Science and Technology of CAAS
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Priority to CN201710404388.5A priority Critical patent/CN107232195A/en
Publication of CN107232195A publication Critical patent/CN107232195A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B9/00—Preservation of edible seeds, e.g. cereals
    • A23B9/16—Preserving with chemicals
    • A23B9/24—Preserving with chemicals in the form of liquids or solids
    • A23B9/26—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3409—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor
    • A23L3/3445—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor in a controlled atmosphere comprising other gases in addition to CO2, N2, O2 or H2O
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3481—Organic compounds containing oxygen
    • A23L3/3499—Organic compounds containing oxygen with doubly-bound oxygen

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides a kind of application of 2 nonanone in aspergillus flavus is suppressed, including the suppression grown to aspergillus flavus, and the suppression to aspergillus spore sprouting.The present invention also proposes a kind of method for suppressing aspergillus flavus using 2 nonanones.The present invention proposes application of 2 nonanones in aspergillus flavus is suppressed.2 nonanones are the food flavor for allowing to use, nontoxic, for that can increase the fragrance of food simultaneously in food, and are had the advantages that cheap and easy to get.The experiment proves that, 2 nonanones can suppress the growth of aspergillus flavus, the sprouting of spore, mycelia is formed and toxin is produced, and as the biological control material of aspergillus flavus, the compound has the application prospect of the new biological pesticide of exploitation or bacteriostatic agent.

Description

A kind of method that utilization methyl n-heptyl ketone suppresses aspergillus flavus
Technical field
The invention belongs to food processing field, and in particular to a kind of aspergillus flavus suppressing method and aspergillus flavus inhibitor.
Background technology
Methyl n-heptyl ketone：English name, 2-Nonanone is colourless oil liquid, water insoluble, is dissolved in the organic flux such as ethanol. It is naturally occurring in cheese, butter oil, strawberry, coconut.With fruity, fragrant fragrant and sweet, wax, soap, blue or green fragrant and coconut, the gas of cream Taste.For allocating the flavoring essences such as jackfruit, coconut, cheese.The FEMA numberings of methyl n-heptyl ketone are that 2785, FDA numberings are 172.515, CoE numberings are 154.Chinese GB 2760-1996 are approved as the food flavor for allowing to use.
Aspergillus flavus (Aspergillus flavus) is a kind of relatively common saprophytic fungus, belongs to half mushroom.Aspergillus flavus (A.flavus) grain oil product such as infecting peanut, corn and nut easily before and after harvest and in storage, causes grain oil product Go mouldy, destroy grain and oil nutritive value, cause grain and oil quality deterioration, cause huge economic loss (Lee and Chuang, 1992；Kayode et al.,2013；Kamala et al.,2015)；What is more important, which is produced, has strong toxicity and carcinogenic Property aflatoxin threaten human and livestock health (Cotty, 1990；Horn andAbbas,2003；Frisvad et al., 2006a；Frisvad et al.,2006b).
Therefore, screening and exploitation can efficiently suppress the safe and nontoxic natural active matter of aspergillus flavus growth, strengthen yellow bent Mould toxin prevention and control research turns into the active demand for ensureing China's grain security and food security.
The content of the invention
The weak point existed for this area, it is an object of the invention to provide methyl n-heptyl ketone answering in aspergillus flavus is suppressed With.
It is another object of the present invention to propose that a kind of utilization methyl n-heptyl ketone suppresses the method for aspergillus flavus.
Third object of the present invention is to propose a kind of aspergillus flavus inhibitor.
The technical scheme for realizing above-mentioned purpose of the present invention is：
Application of the methyl n-heptyl ketone in aspergillus flavus is suppressed, including the suppression grown to aspergillus flavus, and aspergillus spore is sprouted Suppression.
A kind of method that utilization methyl n-heptyl ketone suppresses aspergillus flavus, agricultural product are placed in the atmosphere containing methyl n-heptyl ketone；The agriculture Product includes nut, peanut, grain.
A preferred technical solution of the present invention is that agricultural product are placed in closed vessel, and methyl n-heptyl ketone is placed with container, Every 2~3L container volumes place 1~10mL methyl n-heptyl ketones.
Or, agricultural product are placed in closed vessel, the agricultural product are fumigated with methyl n-heptyl ketone.
Or, contact aspergillus flavus bacterium colony with methyl n-heptyl ketone；The mode of contact is to be placed in the atmosphere containing methyl n-heptyl ketone, or contact It is soaked with the porous material of methyl n-heptyl ketone.
Alternatively, every 50~90cm2The porous material of area is soaked with 50 μ L methyl n-heptyl ketones, the porous material be absorbent cotton, One kind in filter paper, cotton, non-woven fabrics, felt, sponge.
A kind of aspergillus flavus inhibitor, its active ingredient includes methyl n-heptyl ketone.
Further, its active ingredient includes methyl n-heptyl ketone, methyl n-heptyl ketone analog and one kind or many in methyl n-heptyl ketone derivative Kind.
The beneficial effects of the present invention are：
The present invention proposes application of the methyl n-heptyl ketone in aspergillus flavus is suppressed.Methyl n-heptyl ketone is the food flavor for allowing to use, nontoxic It is harmless, in food the fragrance of food can be increased simultaneously, and have the advantages that cheap and easy to get.
The experiment proves that, methyl n-heptyl ketone can suppress the growth of aspergillus flavus, the sprouting of spore, mycelia is formed and toxin is produced Raw, as the biological control material of aspergillus flavus, the compound has the application prospect of the new biological pesticide of exploitation or bacteriostatic agent.
Brief description of the drawings
Fig. 1 is the suppression experiment photo that methyl n-heptyl ketone grows to aspergillus flavus.
Fig. 2 is the suppression experiment photo that methyl n-heptyl ketone is sprouted to aspergillus spore.
Fig. 3 is that methyl n-heptyl ketone tests photo to the suppression that aspergillus flavus on peanut grows.
Embodiment
With reference to specific embodiment, the present invention is further elaborated, but the present invention is not limited to following examples.Should When, it is noted that to those skilled in the art, without departing from the inventive concept of the premise, if can also make Dry modification and improvement, these belong to protection scope of the present invention.
The method used is conventional method unless otherwise instructed.
Embodiment 1：Methyl n-heptyl ketone suppresses the measure of aspergillus flavus growth result
PDA culture medium：Peeled potatoes 200g, which boils, takes filtrate after 30min, glucose 20g, agar 16g are added, with steaming Distilled water is settled to 1000mL, plus deionized water supplies 1L, 115 DEG C of autoclavings 30 minutes.
PDB culture mediums：Peeled potatoes 200g, which boils, takes filtrate after 30min, add glucose 20g, use distilled water constant volume To 1000mL, plus deionized water supplies 1L, 115 DEG C of autoclavings 30 minutes.
WA culture mediums：Agar (15g/L), plus deionized water supply 1L, 121 DEG C of autoclavings 20 minutes.
Actication of culture：Aspergillus flavus is inoculated with PDA culture medium, being grown at 28 DEG C 7 days, 4 DEG C are stored.
Methyl n-heptyl ketone is carried out to aspergillus flavus growth inhibition test using culture dish (diameter 9cm) make-up cultivation.By aspergillus flavus Spore inoculating shakes 3 days generation hypha bodies of training in PDB culture mediums, extracts the hypha body of white, is inoculated in PDA centers.WA culture mediums Sterilizing filter paper piece is placed in center, and 50 μ L methyl n-heptyl ketone is slowly added on filter paper, is test group；Blank group is in WA culture medium The filter paper without methyl n-heptyl ketone of centre, every group of setting 3 is parallel.Fill hypha body PDA culture medium culture dish remove lid, separately The WA medium culture wares of one dress experiment or blank remove lid, and two culture dish make-ups, culture medium contact is sealed with sealed membrane After be put into valve bag, be placed in incubator 28 DEG C of cultures, the growing state of aspergillus flavus is observed in culture after 3 days.
As a result as shown in figure 1, A is control group, B is experimental group.As a result show, methyl n-heptyl ketone can completely inhibit the life of aspergillus flavus It is long.
The methyl n-heptyl ketone of embodiment 2 suppresses the measure that aspergillus spore sprouts effect
Prepare spore:In the Tween 80 that the appropriate aspergillus spore to sterile 0.1% activated is dipped with sterilized cotton swabs, Uniform spore liquid is made in vortex concussion, and blood counting chamber is counted, and spore concentration is diluted into 1 × 105/mL as needed standby With.
Methyl n-heptyl ketone is carried out to aspergillus spore germination inhibitor using culture dish (diameter 9cm) make-up cultivation.100μl Aspergillus spore 1 × 105PDA culture medium is coated on, WA culture medium centrals place sterilizing filter paper piece, and 50 μ L methyl n-heptyl ketone is slow Add on filter paper；The filter paper of blank group is without methyl n-heptyl ketone, and every group of setting 3 is parallel, after culture dish is sealed with sealed membrane It is put into valve bag, is placed in 28 DEG C of cultures in incubator, the spore germination situation of aspergillus flavus is observed in culture after 1 day.
Shown in result figure 2, A is control group, and B is experimental group.Methyl n-heptyl ketone can completely inhibit aspergillus spore and sprout and mycelia Formed.
The methyl n-heptyl ketone of embodiment 3 is determined to the inhibition of aspergillus flavus
Prepare spore:In the Tween 80 that the appropriate aspergillus spore to sterile 0.1% activated is dipped with sterilized cotton swabs, Uniform spore liquid is made in vortex concussion, and blood counting chamber is counted, and spore concentration is diluted into 5 × 10 as needed5Individual/mL is standby With.
Weigh 200g Ecological Property of Peanut Seeds to be stored in 500ml triangular flasks, in 121 DEG C of autoclavings 20 minutes.It is inoculated with aspergillus flavus spore Sub- liquid 2ml, fully shaking 10min are mixed.The seed in triangular flask is poured into 6cm culture dishes at random after shaking up, each culture Corn kernel 25-30g in ware.Inhibition experiment is carried out in two 2.5L driers.Trained on dividing plate in each drier Support 3 parts of peanut samples.6ml methyl n-heptyl ketone is added in experimental group drier bottom.Cover, be placed in 28 DEG C of cultures 7 in incubator My god.
The extraction of aflatoxin：Peanut after culture, is ground after being carried out 121 DEG C of sterilizing 30min with Universalpulverizer Powder, grinding is after drying and processing 5d at 60 DEG C, with reference to immune affinity column operation instructions, using affine in immunity post detection aspergillus flavus The content of toxin.
The detection of aflatoxin：Obtained sample is extracted with HPLC (Post-column photochemical derivatization) to purification to detect, HPLC analyses are carried out to aflatoxin B1 standard items simultaneously.
HPLC testing conditions are mobile phase methanol: water=1: 1 (volume ratio)；Flow velocity 1ml/min；Chromatographic column C18 (150mm × 4.6mm, 0.5 μm)；Excitation wavelength 350nm, Detection wavelength 450nm；Column temperature：30℃；The μ l of sample size 20.
Shown in result figure 3, A is control group, and B is experimental group.Methyl n-heptyl ketone can completely inhibit growth of the aspergillus flavus on peanut It is malicious (table 1) with production.
Aflatoxin content in the peanut of table 1
Group Aflatoxin content (μ g/kg)
Control group Do not detect
Experimental group 38.8±8.4
Embodiment above is only that the preferred embodiment of the present invention is described, and not the scope of the present invention is entered Row is limited, on the premise of design spirit of the present invention is not departed from, technical side of this area ordinary skill technical staff to the present invention In all variations and modifications that case is made, the protection domain that claims of the present invention determination all should be fallen into.

Claims (8)

  1. Application of the 1.2- nonanones in aspergillus flavus is suppressed, it is characterised in that including the suppression grown to aspergillus flavus, and to aspergillus flavus The suppression of spore germination.
  2. 2. a kind of method that utilization methyl n-heptyl ketone suppresses aspergillus flavus, it is characterised in that agricultural product are placed in the atmosphere containing methyl n-heptyl ketone In；The agricultural product include nut, peanut, grain.
  3. 3. the method that utilization methyl n-heptyl ketone according to claim 2 suppresses aspergillus flavus, it is characterised in that agricultural product are placed in envelope Close in container, methyl n-heptyl ketone is placed with container, every 2~3L container volumes place 1~10mL methyl n-heptyl ketones.
  4. 4. the method that utilization methyl n-heptyl ketone according to claim 2 suppresses aspergillus flavus, it is characterised in that by aspergillus flavus bacterium colony with Methyl n-heptyl ketone is contacted；The mode of contact is to be placed in the atmosphere containing methyl n-heptyl ketone, or contact is soaked with the porous material of methyl n-heptyl ketone.
  5. 5. the method that utilization methyl n-heptyl ketone according to claim 4 suppresses aspergillus flavus, it is characterised in that every 50~90cm2Area Porous material be soaked with 50 μ L methyl n-heptyl ketones, during the porous material is absorbent cotton, filter paper, cotton, non-woven fabrics, felt, sponge It is a kind of.
  6. 6. the method that utilization methyl n-heptyl ketone according to claim 2 suppresses aspergillus flavus, it is characterised in that agricultural product are placed in envelope Close in container, the agricultural product are fumigated with methyl n-heptyl ketone.
  7. 7. a kind of aspergillus flavus inhibitor, it is characterised in that its active ingredient includes methyl n-heptyl ketone.
  8. 8. aspergillus flavus inhibitor according to claim 7, it is characterised in that its active ingredient includes methyl n-heptyl ketone, methyl n-heptyl ketone One or more in analog and methyl n-heptyl ketone derivative.
CN201710404388.5A 2017-06-01 2017-06-01 A kind of method for suppressing aspergillus flavus using 2 nonanones Pending CN107232195A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110407696A (en) * 2019-07-10 2019-11-05 中国农业科学院农产品加工研究所 Benzo cyclopentanone derivatives and the preparation method and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5334619A (en) * 1993-07-07 1994-08-02 The United States Of America As Represented By The Secretary Of Agriculture Inhibition of postharvest fruit decay by 2-nonanone
WO2007103336A2 (en) * 2006-03-06 2007-09-13 The Board Of Trustees Operating Micro-encapsulation of volatile compounds into cyclodextrins
WO2014165969A1 (en) * 2013-04-12 2014-10-16 Perry Lidster Antimicrobial mixtures of an essential oil derived bactericide and an essential oil derived fungicide which extend the shelf-life of perishable agricultural commodities
JP5907655B2 (en) * 2009-12-21 2016-04-26 テーブルマーク株式会社 Food preservative, method for inhibiting mold and / or yeast growth, method for inhibiting yeast and / or bacterial growth

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5334619A (en) * 1993-07-07 1994-08-02 The United States Of America As Represented By The Secretary Of Agriculture Inhibition of postharvest fruit decay by 2-nonanone
WO2007103336A2 (en) * 2006-03-06 2007-09-13 The Board Of Trustees Operating Micro-encapsulation of volatile compounds into cyclodextrins
JP5907655B2 (en) * 2009-12-21 2016-04-26 テーブルマーク株式会社 Food preservative, method for inhibiting mold and / or yeast growth, method for inhibiting yeast and / or bacterial growth
WO2014165969A1 (en) * 2013-04-12 2014-10-16 Perry Lidster Antimicrobial mixtures of an essential oil derived bactericide and an essential oil derived fungicide which extend the shelf-life of perishable agricultural commodities

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
H. J. ZERINGUE JR. AND S. P. MCCORMICK: "Relationships Between Cotton Leaf-Derived Volatiles and Growth of Aspergiflus flavus", 《JAOCS》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110407696A (en) * 2019-07-10 2019-11-05 中国农业科学院农产品加工研究所 Benzo cyclopentanone derivatives and the preparation method and application thereof
CN110407696B (en) * 2019-07-10 2021-09-07 中国农业科学院农产品加工研究所 Benzocyclopentanone derivative and preparation method and application thereof

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Application publication date: 20171010