CN107232054A - The tissue-culturing quick-propagation device and its application method of a kind of succulent jade dew - Google Patents
The tissue-culturing quick-propagation device and its application method of a kind of succulent jade dew Download PDFInfo
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- CN107232054A CN107232054A CN201710447866.0A CN201710447866A CN107232054A CN 107232054 A CN107232054 A CN 107232054A CN 201710447866 A CN201710447866 A CN 201710447866A CN 107232054 A CN107232054 A CN 107232054A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
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Abstract
The invention discloses a kind of tissue-culturing quick-propagation device of succulent jade dew and its application method, device includes central control room and annular arrangement in the explant disinfection room outside central control room, inducing clumping bud room and culture of rootage room, the explant sterilization chamber interior walls set cleaning beaker fixed in multiple necks and embedded neck from the bottom to top, stock is provided with the explant disinfection room, the body of rod of the stock is provided with the explant bearing end stretched into each cleaning beaker, the inducing clumping bud room is with being provided with ambient signal harvester in culture of rootage room, aid in illumination apparatus, temperature control device and humidity conditioner, central controller is provided with inside the central control room, human-computer interaction interface device is provided with the top of central control room.The present invention can quickly breed a large amount of tissue-cultured seedling for being adapted to transplant, and proliferation frequency is high, and seedling is neat, meets production requirement, improves economic benefit.
Description
Technical field
The present invention relates to the tissue-culturing quick-propagation of plant tissue culture technical field, more particularly to a kind of succulent jade dew
Device and application method.
Background technology
Jade dew belongs to fleshiness plant by volume 12 for American aloe mesh Eremurus chinensis section (also referred to as Asphodelaceae, Aloeaceae or A Fu flowers section)
Soft leaf system kind in thing, originates in South Africa, this life circle, many ground can be cultivated.It is Dan Sheng at the beginning of plant, is in gradually all living creatures's shape, meat later
Matter leaf is arranged in rosette-stape, and both sides garden is convex；Blade tip is transparent or translucent, is referred to as " window ", and there is longitudinal dark strokes on surface, pushes up
There is tiny palpus at end, and leaf color is dark green；Loose raceme, small floral white has green vertical stripe.Jade reveals small and exquisite, blade
It is glittering and translucent, it is peculiar and beautiful as jade carving is formed, it is very lovely such as lived handicraft, with small potted plant kind of point
Sew desk, desk, windowsill etc., Fresh and elegant is rare indoor appreciation flowers, be the more prosperous small-sized fleshiness of popularity in recent years
One of plant variety, by succulent, fan is pursued, with good market prospects and economic value.
The common propagation method of jade dew has cuttage, plant division, leaf to insert and the several method such as sowing.But for jade dew, this
A little method reproduction speeds are slower, and less efficient, breeding coefficient is low, and the breeding cycle is long, and site requirements is big, and cost of labor is higher.Cause
This, improves the production efficiency of succulent jade dew, shortens the cultivation time, and reduction cost of labor turns into urgently to be resolved hurrily at present and asked
Topic.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of succulent jade dew tissue-culturing quick-propagation device and
Its application method, the device is by explant disinfection room, inducing clumping bud room and the part of culture of rootage room three to jade dew explant
Body carries out culture and obtains tissue-cultured seedling, improves the reproductive efficiency of succulent jade dew, its incubation time is short, and cost of labor is low.
To solve the above problems, the present invention is adopted the following technical scheme that：
The invention provides a kind of succulent jade dew tissue-culturing quick-propagation device, it include central control room with
And annular arrangement is in the explant disinfection room outside central control room, inducing clumping bud room and culture of rootage room, wherein the center
Control room includes panel one, panel two and the panel for corresponding to explant disinfection room, inducing clumping bud room and culture of rootage room respectively
Three, the explant sterilization chamber interior walls set cleaning beaker fixed in multiple necks and embedded neck from the bottom to top, described
Stock is embedded with panel one, the body of rod of the stock is provided with the explant bearing end stretched into each cleaning beaker, the clump
Induction room of sprouting is respectively equipped with neck and is embedded at the culture medium of neck with culture of rootage chamber interior walls, and the culture medium is externally provided with ring
Border signal pickup assembly, the panel two is provided with auxiliary illumination apparatus, temperature control device and humidity conditioner with panel three,
It is provided with inside the central control room at the top of central controller, central control room and is provided with human-computer interaction interface device, it is described man-machine
Interactive interface device, ambient signal harvester, auxiliary illumination apparatus, temperature control device and humidity conditioner are all connected with central control
Device processed.
Further, the central control room is triangular structure, and on each side provided with buckling groove, the explant sterilization
Room, inducing clumping bud room and culture of rootage room are fixedly connected on the outside of central control room by the buckling groove.
Further, the explant sterilization chamber interior walls are provided with three necks and three cleaning beakers from the bottom to top.
Further, the ambient signal harvester includes optical sensor, temperature sensor and humidity sensor.
Further, culture medium is adventitious shoots culture base in the inducing clumping bud room, and its component is：MS is cultivated substantially
Base, 0.01~0.08mg/L of supplement NAA, 2.0~5.0mg/L6-BA, 0.1~0.8g/L caseinhydrolysates, 10~40g/L sugarcanes
Sugar, 2~10g/L agar.
Further, culture medium is root media in the culture of rootage room, and the composition of the root media is：1/
4MS minimal mediums, 001~0.08mg/L of supplement NAA, 10~40g/L sucrose, 2~10g/L agar, 0.1~0.5mg/L
Activated carbon.
Present invention also offers the application method of the tissue-culturing quick-propagation device of above-mentioned succulent jade dew, specific bag
Include following steps：
(1) required antiseptic solution is poured into each cleaning beaker into explant disinfection room, with the jade cleaned up
Dew blade is explant, beautiful dew explant is put into the explant bearing end in explant disinfection room, rotating and carrying is made by stock
Jade dew explant immerses sterilization and cleaning in each beaker and obtains aseptic explant successively in order；
(2) aseptic explant is cut into 0.3~0.6cm section, is inoculated on adventitious shoots culture base, is controlled using center
When the human-computer interaction interface device of room presets inducing clumping bud indoor lighting conditions for 1100~1300lux of intensity of illumination, illumination
Between be 12~16 hours, temperature be 24~26 DEG C, relative humidity be 50%~60%, in inducing clumping bud room cultivate 15~
18 days, obtain Multiple Buds；
(3) directly it is transferred to after Multiple Buds are separated by 3~4 buds for one plant in root media, utilizes central control room
Human-computer interaction interface device to preset culture of rootage indoor lighting conditions be that 1100~1300lux of intensity of illumination, light application time are
12~16 hours, temperature was 24~26 DEG C, and relative humidity is 50%~60%, cultivates 3~6 weeks, obtains in culture of rootage room
Intact plant with root.
Preferably, the antiseptic solution of the step (1) includes the mercury chloride and mass fraction 2%~3% of mass fraction 1%
Aqueous sodium hypochlorite solution.
Compared to prior art, the beneficial effects of the present invention are：Tissue-culturing quick-propagation device includes in the present invention
Central control room, explant disinfection room, inducing clumping bud room and culture of rootage room, explant disinfection room, inducing clumping bud room and
Culture of rootage room can be quickly connected on central control room by buckling groove or be removed from central control room, consequently facilitating right
The preparation with thimerosal is fixed in cleaning beaker in explant disinfection room, and is easy to the access adventitious shoots culture of explant
Base, Multiple Buds access root media；Central control room of the present invention passes through human-computer interaction interface device default beautiful each stage of dew
Required ambient parameter (temperature, humidity and intensity of illumination), and then environment signal pickup assembly institute is received instead by central controller
The ambient parameter (temperature, humidity and intensity of illumination) of feedback, and control auxiliary illumination apparatus, temperature control device and humidity conditioner work
Make so that inducing clumping bud room and culture of rootage room are maintained in the range of default ambient parameter, thus reach reduction it is artificial into
Originally the purpose of the reproductive efficiency of beautiful dew, is improved.The present invention can quickly breed a large amount of tissue-cultured seedling for being adapted to transplant, and meet production
Demand, improves economic benefit.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the tissue-culturing quick-propagation device of succulent jade dew of the present invention；
Fig. 2 is the sectional structure chart of explant disinfection room in Fig. 1；
Fig. 3 is the sectional structure chart of inducing clumping bud room and culture of rootage room in Fig. 1；
Fig. 4 is control process principle schematic of the invention；
Wherein, 1-central control room, 2-explant disinfection room, 3-inducing clumping bud room, 4-culture of rootage room,
21-panel one, 31-panel two, 41-panel three, 5-neck, 6-cleaning beaker, 7-stock, the carrying of 8-explant
End, 9-culture medium, 10-ambient signal harvester, 11-auxiliary illumination apparatus, 12-temperature control device, 13-humid control
Device, 14-human-computer interaction interface device, 15-central controller, 16-buckling groove, 101-optical sensor, 102-temperature
Spend sensor, 103-humidity sensor.
Embodiment
The present invention is further detailed explanation with reference to the accompanying drawings and examples.
A kind of tissue-culturing quick-propagation device of succulent jade dew as Figure 1-Figure 4, it includes center control
Room 1 and annular arrangement in the explant disinfection room 2 outside central control room 1, inducing clumping bud room 3 and culture of rootage room 4, its
Described in central control room 1 include corresponding to explant disinfection room 2, inducing clumping bud room 3 and the panel one of culture of rootage room 4 respectively
21st, panel 2 31 and panel 3 41, the inwall of explant disinfection room 1 set multiple necks 5 and embedded neck 5 from the bottom to top
Stock 7 is embedded with the cleaning beaker 6 of interior fixation, the panel 1, the body of rod of the stock 7 burns provided with each cleaning is stretched into
Explant bearing end 8 in cup 6, the inducing clumping bud room 3 is respectively equipped with neck 5 with the inwall of culture of rootage room 4 and is embedded at
The culture medium 9 of neck 5, the culture medium 9 is externally provided with ambient signal harvester 10, and the panel 2 31 is all provided with panel 3 41
Auxiliary illumination apparatus 11, temperature control device 12 and humidity conditioner 13 are equipped with, the inside of central control room 1 is provided with center control
Device 15 processed, the top of central control room 1 is provided with human-computer interaction interface device 14, the man-machine interaction group interface device 14, environment letter
Number harvester 10, auxiliary illumination apparatus 11 (LED fluorescent lamps are used in this example), temperature control device 12 are (using electricity in this example
Resistance heater) and humidity conditioner 13 (in this example use air humidifier) be all connected with central controller 15.
In this example, the central control room 1 is triangular structure, and is provided with buckling groove 16, the explant on each side
Body disinfection room 2, inducing clumping bud room 3 and culture of rootage room 4 are fixedly connected on outside central control room 1 by the buckling groove 16
Side.
In this example, the inwall of explant disinfection room 2 is provided with three necks 5 from the bottom to top and three cleanings are burnt
Cup 6.
In this example, the ambient signal harvester 10 includes optical sensor 101, temperature sensor 102 and wet
Spend sensor 103.
In this example, culture medium 9 is adventitious shoots culture base in the inducing clumping bud room 3, and its component is：MS is trained substantially
Support base, 0.01~0.08mg/L of supplement NAA, 2.0~5.0mg/L6-BA, 0.1~0.8g/L caseinhydrolysates, 10~40g/L
Sucrose, 2~10g/L agar.
In this example, culture medium 9 is root media, the composition of the root media in the culture of rootage room 4
For：1/4MS minimal mediums, supplement 001~0.08mg/L NAA, 10~40g/L sucrose, 2~10g/L agar, 0.1~
0.5mg/L activated carbons.
The application method of the tissue-culturing quick-propagation device of above-mentioned succulent jade dew, specifically includes following steps：
(1) required antiseptic solution is poured into each cleaning beaker 6 into explant disinfection room 1, with what is cleaned up
Jade dew blade is explant, and beautiful dew explant is put into the explant bearing end 8 in explant disinfection room 1, rotates and carries by length
Bar 7 makes beautiful dew explant immerse sterilization and cleaning in each beaker 6 successively in order to obtain aseptic explant；
(2) aseptic explant is cut into 0.3~0.6cm section, is inoculated on adventitious shoots culture base, is controlled using center
It is 1100~1300lux of intensity of illumination, light that the human-computer interaction interface device 14 of room 1, which presets illumination condition in inducing clumping bud room 3,
It it is 12~16 hours according to the time, temperature is 24~26 DEG C, relative humidity is 50%~60%, is cultivated in inducing clumping bud room 3
15~18 days, obtain Multiple Buds；
(3) directly it is transferred to after Multiple Buds are separated by 3~4 buds for one plant in root media, utilizes central control room 1
Human-computer interaction interface device 14 to preset in culture of rootage room 4 illumination condition be 1100~1300lux of intensity of illumination, light application time
For 12~16 hours, temperature was 24~26 DEG C, and relative humidity is 50%~60%, culture 3~6 weeks in culture of rootage room 4,
Obtain the intact plant with root.
In this example, the antiseptic solution of the step (1) includes the mercury chloride and mass fraction 2% of mass fraction 1%
~3% aqueous sodium hypochlorite solution.
Based on above-mentioned, central control room 1 of the present invention pass through human-computer interaction interface device 14 it is default it is beautiful reveal each stage needed for
Ambient parameter (temperature, humidity and intensity of illumination), and then it is anti-by central controller 15 to receive the institute of environment signal pickup assembly 10
The ambient parameter (temperature, humidity and intensity of illumination) of feedback, and control auxiliary illumination apparatus 11, temperature control device 12 and humid control dress
13 work are put so that inducing clumping bud room 3 and culture of rootage room 4 are maintained in the range of default ambient parameter.
Those of ordinary skill in the art it should be appreciated that the embodiment of the above be intended merely to explanation the present invention,
And be not used as limitation of the invention, as long as in the spirit of the present invention, the change to embodiment described above
Change, modification will all fall in scope of the presently claimed invention.
Claims (8)
1. a kind of tissue-culturing quick-propagation device of succulent jade dew, it is characterised in that including central control room and ring
Shape is arranged in explant disinfection room, inducing clumping bud room and culture of rootage room outside central control room, wherein the center is controlled
Room includes corresponding to panel one, panel two and the panel three of explant disinfection room, inducing clumping bud room and culture of rootage room, institute respectively
State explant sterilization chamber interior walls and cleaning beaker fixed in multiple necks and embedded neck, the panel one are set from the bottom to top
Stock is inside embedded with, the body of rod of the stock is provided with the explant bearing end stretched into each cleaning beaker, and the Multiple Buds are lured
Lead room to be respectively equipped with neck with culture of rootage chamber interior walls and be embedded at the culture medium of neck, the culture medium is externally provided with ambient signal
Harvester, the panel two and panel three are provided with auxiliary illumination apparatus, temperature control device and humidity conditioner, it is described in
It is provided with inside heart control room at the top of central controller, central control room and is provided with human-computer interaction interface device, man-machine interaction circle
Face device, ambient signal harvester, auxiliary illumination apparatus, temperature control device and humidity conditioner are all connected with central controller.
2. a kind of tissue-culturing quick-propagation device of succulent jade dew according to claim 1, it is characterised in that institute
Central control room is stated for triangular structure, and buckling groove, the explant disinfection room, inducing clumping bud room and life are provided with each side
Root culturing room is fixedly connected on the outside of central control room by the buckling groove.
3. a kind of tissue-culturing quick-propagation device of succulent jade dew according to claim 1, it is characterised in that institute
State explant sterilization chamber interior walls and be provided with three necks and three cleaning beakers from the bottom to top.
4. a kind of tissue-culturing quick-propagation device of succulent jade dew according to claim 1, it is characterised in that institute
Stating ambient signal harvester includes optical sensor, temperature sensor and humidity sensor.
5. a kind of tissue-culturing quick-propagation device of succulent jade dew according to claim 1, it is characterised in that institute
It is adventitious shoots culture base to state culture medium in inducing clumping bud room, and its component is：MS minimal mediums, supplement 0.01~0.08mg/L
NAA, 2.0~5.0mg/L6-BA, 0.1~0.8g/L caseinhydrolysates, 10~40g/L sucrose, 2~10g/L agar.
6. a kind of tissue-culturing quick-propagation device of succulent jade dew according to claim 1, it is characterised in that institute
It is root media to state culture medium in culture of rootage room, and the composition of the root media is：1/4MS minimal mediums, supplement
001~0.08mg/L NAA, 10~40g/L sucrose, 2~10g/L agar, 0.1~0.5mg/L activated carbons.
7. a kind of user of the tissue-culturing quick-propagation device of succulent jade dew as described in claim any one of 1-6
Method, it is characterised in that specifically include following steps：
(1) required antiseptic solution is poured into each cleaning beaker into explant disinfection room, with the beautiful dew leaf cleaned up
Piece is explant, beautiful dew explant is put into the explant bearing end in explant disinfection room, rotating and carrying makes beautiful dew by stock
Explant immerses sterilization and cleaning in each beaker and obtains aseptic explant successively in order；
(2) aseptic explant is cut into 0.3~0.6cm section, is inoculated on adventitious shoots culture base, utilizes central control room
It is that 1100~1300lux of intensity of illumination, light application time are that human-computer interaction interface device, which presets inducing clumping bud indoor lighting conditions,
12~16 hours, temperature was 24~26 DEG C, and relative humidity is 50%~60%, is cultivated 15~18 days in inducing clumping bud room,
Obtain Multiple Buds；
(3) directly it is transferred to after Multiple Buds are separated by 3~4 buds for one plant in root media, utilizes the people of central control room
Machine interactive interface device preset culture of rootage indoor lighting conditions be 1100~1300lux of intensity of illumination, light application time be 12~
16 hours, temperature was 24~26 DEG C, and relative humidity is 50%~60%, is cultivated 3~6 weeks in culture of rootage room, obtained band root
Intact plant.
8. the application method of the tissue-culturing quick-propagation device of succulent jade dew according to claim 7, its feature
It is, the antiseptic solution of the step (1) includes the mercury chloride of mass fraction 1% and the sodium hypochlorite of mass fraction 2%~3%
The aqueous solution.
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| CN201710447866.0A CN107232054A (en) | 2017-06-14 | 2017-06-14 | The tissue-culturing quick-propagation device and its application method of a kind of succulent jade dew |
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Cited By (1)
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| CN108401911A (en) * | 2018-06-13 | 2018-08-17 | 内蒙古自治区生物技术研究院 | Jade dew tissue culture medium (TCM) and preparation method thereof |
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Application publication date: 20171010 |