CN107224621A - One kind has endothelium bionic function coating and preparation method thereof - Google Patents

One kind has endothelium bionic function coating and preparation method thereof Download PDF

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Publication number
CN107224621A
CN107224621A CN201610172970.9A CN201610172970A CN107224621A CN 107224621 A CN107224621 A CN 107224621A CN 201610172970 A CN201610172970 A CN 201610172970A CN 107224621 A CN107224621 A CN 107224621A
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wsc
liquaemin
endothelium
solution
coating
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黄楠
齐鹏凯
杨志禄
杨莹
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CHENGDU JIAODA MAIDIKE TECHNOLOGY Co Ltd
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CHENGDU JIAODA MAIDIKE TECHNOLOGY Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/08—Materials for coatings
    • A61L31/10—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/28—Materials for coating prostheses
    • A61L27/34—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L31/16—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L33/00—Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
    • A61L33/0005—Use of materials characterised by their function or physical properties
    • A61L33/0011—Anticoagulant, e.g. heparin, platelet aggregation inhibitor, fibrinolytic agent, other than enzymes, attached to the substrate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L33/00—Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
    • A61L33/06—Use of macromolecular materials
    • A61L33/08—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/21—Acids
    • A61L2300/214—Amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/23—Carbohydrates
    • A61L2300/236—Glycosaminoglycans, e.g. heparin, hyaluronic acid, chondroitin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/42—Anti-thrombotic agents, anticoagulants, anti-platelet agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/422—Anti-atherosclerotic agents

Abstract

There is endothelium bionic function coating and preparation method thereof the invention discloses one kind, for cardiovascular implant and contacting blood material surface modifying, the compound of disulfide bond containing carboxyl with nitric oxide catalytic activity and heparin are fixed on the coating surface rich in amido altogether.The functional coating shows class endothelium bionic function, not only show excellent anticoagulation function, but also have both the excellent function of suppressing smooth muscle cell proliferation and promotion endothelial cell growth, it can be widely applied to the processing of the medical apparatus surfaces such as intravascular stent, artificial blood vessel, extracorporal circulatory system conduit, CVC.

Description

One kind has endothelium bionic function coating and preparation method thereof
Technical field
The present invention relates to bio-medical engineering material, the technology with endothelium bionic function coating is especially prepared.
Background technology
Angiocardiopathy, it is generally relevant with atherosclerosis, it is to cause human death and the master disabled in world wide Want reason.Since Sigwart postoperative, the metallic blood vessels that reported first case human coronary stenter to implant in 1987 Stent has turned into a kind of one of means of most common treatment severe cardiovascular disease.But, stent-expansion holds Vascular tissue around easy damaged implantation.Vascular tissue's wound causes thrombosis, inflammation, vascellum endometrial hyperplasia and base Matter deposition/vascular remodeling, 3-6 month implanting results show that in-stent restenosis rate is up to 20-30%.Artificial blood vessel Especially small-caliber artificial blood vessel (<Application 5mm) also results in post-surgical vascular damage location smooth muscle cell proliferation, Again result in the generation of ISR.Extracorporal circulatory system conduit is outside a certain degree of anticoagulation function of guarantee, long-term biology Compatibility is not good, can also cause the generation of the accidents such as infection.So being carried out for angiocarpy with medical material bionical natural The biocompatibility that endothelialization processing improves material is reduction restenosis rate, reduces one of important means of postoperative infection.
It is used as a kind of preferable angiocarpy support and tube material, it should while there is excellent anticoagulation, promote endothelium Cell growth and the ability for suppressing smooth muscle proliferation.However, most at present change for improving cardiovascular material surface Property strategy focus only on and improve material biocompatibility in a certain respect.It is temporarily implanted effect significantly, but for a long time Clinical follow up results comply with one's wishes not to the utmost.For example, being once described as having guided the drug eluting vascular of new round intravascular stent revolution The appearance of support has but triggered even more serious advanced thrombus although largely reducing support in stenosis rate.This It is because stents releasing drugs also suppress the growth of endothelial cell while smooth muscle cell proliferation is suppressed, so as to delay Rack surface endothelialization process again.The complete blood vessel endothelial layer of one health passes through numerous pathologic, physiologic work( Energy (such as Heparan sulfate and prostacyclin) includes sustained release nitric oxide (NO) to maintain the normal heart Vascular function is such as：Vasodilation, inhibition thrombosis suppresses smooth muscle proliferation etc..
The content of the invention
In view of above-mentioned the deficiencies in the prior art, the purpose of the present invention is to obtain a kind of simple method to prepare with interior The coating of skin bionic function, to meet its anti-freezing, anti-infective, promotion endothelial cell growth, suppression in use The demand of proliferation of smooth muscle.It can be widely applied to cardiovascular implantation intervention biomaterial including but not limited to medical metal-based Material (Fe and its alloy, mg-based material, 316L SS, Ti, Ti alloy Ni-Ti alloys and CoCr alloy etc.), Inorganic material (Ti-O, TiN, pyrolytic carbon, aluminum oxide, hydroxyapatite, calcium phosphate etc.), high polymer material are (such as： PET, PTFE, PDMS etc.) and degradable high polymer material (such as PLA, PLGA, PCL, PTMC) surface It is modified.
The purpose of the present invention is realized by following technological means：One kind has endothelium bionic function coating, and its feature exists In in biomaterial the equipment surfaces simultaneously fixed compound of disulfide bond containing carboxyl and liver with nitric oxide catalytic activity Element.
The biomaterial includes but is not limited to the biomaterial for cardiovascular implant material, including：Medical metal Sill, inorganic material, high polymer material and degradable surface modified polymer material.
The medical metal sill includes：Fe and its alloy, mg-based material, 316L SS, Ti, Ni-Ti alloys And CoCr alloy.
The present invention also aims to obtain the preparation method with endothelium bionic function coating, it will be urged with nitric oxide The compound of disulfide bond containing carboxyl and the heparin for changing activity are fixed on the biomaterial surface rich in amido altogether, including it is following its Step is：
A, amine-containing functional films preparation
In coating of the biomaterial surface formation rich in amido of including but not limited to cardiovascular implant material；
B, the compound of disulfide bond containing carboxyl and heparin be fixed on the biomaterial surface rich in amido altogether, A is made Surface rich in amido coating biomaterial be soaked in the compound of disulfide bond containing carboxyl/heparin-WSC solution react, Then fully rinsed with buffer solution and distilled water respectively, target is made after drying has endothelium bionic function coating.
In actually implementing, method of the invention can selection or optimization as needed, i.e.,：
The step A is made coating and includes but is not limited to following form：Plasma polymerized coating, Polyphenols and polyamines Cardinal extremity molecule copolymerization coating, Electrostatic Absorption rich amine base molecular coatings, the silane coupled terminal modified coating of amido.
The compound of disulfide bond containing carboxyl includes but is not limited to material：3,3- dithiodipropionic acids, cystine, sulphur is pungent Acid, meso-DMSA and liquaemin and is dissolved in WSC solution, and WSC reaction solutions, which are constituted, is 2- (N- morpholines) ethyl sulfonic acid (MES), 1- ethyls -3- (3- dimethylamino-propyls) carbodiimide (EDC) and N- hydroxysuccinimides (NHS).
Compared with prior art, the beneficial effects of the invention are as follows：It is simultaneously fixed with an oxidation in cardiovascular material surface The DiThi and heparin of nitrogen catalytic activity, it shows high endothelium bionic function, possesses anti-freezing, promotees endothelium suppression smoothly The multi-biological function such as flesh and treatment atherosclerosis, greatly improves the success of cardiovascular implantation instrument implantation Rate.
Its advantage is：
First, by DiThi and heparin by the EDC/NHS carboxyls activated and film surface reactivity amido contract Reaction is closed, fixed DiThi and heparin have very strong adhesion and chemical stability.
2nd, because heparin and NO are two big fundamentals of maintenance normal endothelial cell function, rack surface is simultaneously common DiThi and heparin with nitric oxide catalytic activity are fixed by the bionical vascular endothelial cell layer in bigger degree Function, can fundamentally improve the biocompatibility of intravascular stent.
The preparation of such a endothelium bionic coating simultaneously, it is only necessary to using simple covalently fixing means in conventional amine-based surface DiThi and heparin molecule layer are built, its preparation process is simple to operate, without expensive special equipment, system Standby cost is low.
Embodiment
The implementation to the present invention is further described below.But it is emphasized that following embodiment is Exemplary, the scope being not intended to be limiting of the invention and application.
Embodiment 1
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By intravascular stent (316L SS, Ti and its alloy, Ti-O, Ti-N, Co-Cr alloy, Fe and its alloy, Zn and its alloy, Mg and its alloy substrates) it is put into film In cvd reactive chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, the argon gas that flow is 0.5-5sccm is passed through As discharge gas, and be passed through makes operating pressure be 1-10Pa as the allylamine of reacting gas, in radio-frequency power 5-10W, back bias voltage be 0-150V, pulse duty factor be when entering behavior under conditions of 5-100% 5-60 minutes etc. from Daughter polyallylamine thin film deposition, i.e., plasma polyallylamine of the deposition rich in amino is thin on intravascular stent surface Film.
B, 3,3- dithiodipropionic acid and liquaemin are fixed altogether
3,3- dithiodipropionic acids are pressed into 0.01 μ g/ml-10 by 0.01 μ g/ml-10mg/ml and liquaemin Mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morpholines) 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml of ethyl sulfonic acid cushioning liquid, 1mg/ml N- hydroxysuccinimides, 3,3- dithiodipropionic acid-WSC and liquaemin-WSC solution are obtained respectively；Place 3,3- 3,3- dithiodipropionic acids/liquaemin is mixed into after dithiodipropionic acid-WSC and liquaemin-WSC solution 1min-12h - WSC solution, 3, the 3- bis- thio two is soaked in by the intravascular stent of surface deposition plasma polyallylamine film React -48 hours 1 hour, then fully rinsed with PBS and distilled water respectively in propionic acid/liquaemin-WSC solution, Dry, produce purpose thing.
The intravascular stent of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after intravascular stent implantation The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 2
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By intravascular stent (316L SS, Ti and its alloy, Ti-O, Ti-N, Co-Cr alloy, Fe and its alloy, Zn and its alloy, Mg and its alloy substrates) it is put into film In cvd reactive chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, the argon gas that flow is 0.5-5sccm is passed through As discharge gas, and be passed through makes operating pressure be 1-10Pa as the allylamine of reacting gas, in radio-frequency power 5-10W, back bias voltage be 0-150V, pulse duty factor be when entering behavior under conditions of 5-100% 5-60 minutes etc. from Daughter polyallylamine thin film deposition, i.e., plasma polyallylamine of the deposition rich in amino is thin on intravascular stent surface Film.
B, cystine and liquaemin are fixed altogether
Cystine is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains cystine-WSC and liquaemin-WSC solution respectively；Place cystine-WSC and liquaemin-WSC Cystine/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the cystine/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The intravascular stent of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after intravascular stent implantation The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 3
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By intravascular stent (316L SS, Ti and its alloy, Ti-O, Ti-N, Co-Cr alloy, Fe and its alloy, Zn and its alloy, Mg and its alloy substrates) it is put into film In cvd reactive chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, the argon gas that flow is 0.5-5sccm is passed through As discharge gas, and be passed through makes operating pressure be 1-10Pa as the allylamine of reacting gas, in radio-frequency power 5-10W, back bias voltage be 0-150V, pulse duty factor be when entering behavior under conditions of 5-100% 5-60 minutes etc. from Daughter polyallylamine thin film deposition, i.e., plasma polyallylamine of the deposition rich in amino is thin on intravascular stent surface Film.
B, lipoic acid and liquaemin are fixed altogether
Lipoic acid is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains lipoic acid-WSC and liquaemin-WSC solution respectively；Place lipoic acid-WSC and liquaemin-WSC Lipoic acid/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the lipoic acid/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The intravascular stent of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after intravascular stent implantation The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 4
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By intravascular stent (316L SS, Ti and its alloy, Ti-O, Ti-N, Co-Cr alloy, Fe and its alloy, Zn and its alloy, Mg and its alloy substrates) it is put into film In cvd reactive chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, the argon gas that flow is 0.5-5sccm is passed through As discharge gas, and be passed through makes operating pressure be 1-10Pa as the allylamine of reacting gas, in radio-frequency power 5-10W, back bias voltage be 0-150V, pulse duty factor be when entering behavior under conditions of 5-100% 5-60 minutes etc. from Daughter polyallylamine thin film deposition, i.e., plasma polyallylamine of the deposition rich in amino is thin on intravascular stent surface Film.
B, meso -2,3- dimercaptosuccinic acids and liquaemin are fixed altogether
Meso -2,3- dimercaptosuccinic acids are pressed into 0.01 μ g/ml by 0.01 μ g/ml-10mg/ml and liquaemin - 10mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morphines Quinoline) ethyl sulfonic acid cushioning liquid, 1mg/ml 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxysuccinimides, meso-DMSA-WSC and liquaemin-WSC solution are obtained respectively； Meso is mixed into after placing meso -2,3- dimercaptosuccinic acid-WSC and liquaemin-WSC solution 1min-12h - DMSA/liquaemin-WSC solution, by the blood vessel branch of surface deposition plasma polyallylamine film Frame is soaked in reaction -48 hours 1 hour in the meso-DMSA/liquaemin-WSC solution, so Fully rinsed with PBS and distilled water respectively afterwards, dry, produce purpose thing.
The intravascular stent of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after intravascular stent implantation The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 5
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, base material is soaked in the solution of dopamine/hexamethylene diamine and reacts 1-48h, is then fully rinsed with distilled water, Dry, produce.
B, 3,3- dithiodipropionic acid and liquaemin are fixed altogether
3,3- dithiodipropionic acids are pressed into 0.01 μ g/ml-10 by 0.01 μ g/ml-10mg/ml and liquaemin Mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morpholines) 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml of ethyl sulfonic acid cushioning liquid, 1mg/ml N- hydroxysuccinimides, 3,3- dithiodipropionic acid-WSC and liquaemin-WSC solution are obtained respectively；Place 3,3- 3,3- dithiodipropionic acids/liquaemin is mixed into after dithiodipropionic acid-WSC and liquaemin-WSC solution 1min-12h - WSC solution, 3, the 3- bis- thio two is soaked in by the intravascular stent of surface deposition plasma polyallylamine film React -48 hours 1 hour, then fully rinsed with PBS and distilled water respectively in propionic acid/liquaemin-WSC solution, Dry, produce purpose thing.
The intravascular stent of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after intravascular stent implantation The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 6
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, base material is soaked in the solution of dopamine/hexamethylene diamine and reacts 1-48h, is then fully rinsed with distilled water, Dry, produce.
B, cystine and liquaemin are fixed altogether
Cystine is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains cystine-WSC and liquaemin-WSC solution respectively；Place cystine-WSC and liquaemin-WSC Cystine/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the cystine/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The intravascular stent of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after intravascular stent implantation The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 7
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, base material is soaked in the solution of dopamine/hexamethylene diamine and reacts 1-48h, is then fully rinsed with distilled water, Dry, produce.
B, lipoic acid and liquaemin are fixed altogether
Lipoic acid is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains lipoic acid-WSC and liquaemin-WSC solution respectively；Place lipoic acid-WSC and liquaemin-WSC Lipoic acid/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the lipoic acid/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The intravascular stent of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after intravascular stent implantation The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 8
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, base material is soaked in the solution of dopamine/hexamethylene diamine and reacts 1-48h, is then fully rinsed with distilled water, Dry, produce.
B, meso -2,3- dimercaptosuccinic acids and liquaemin are fixed altogether
Meso -2,3- dimercaptosuccinic acids are pressed into 0.01 μ g/ml by 0.01 μ g/ml-10mg/ml and liquaemin - 10mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morphines Quinoline) ethyl sulfonic acid cushioning liquid, 1mg/ml 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxysuccinimides, meso-DMSA-WSC and liquaemin-WSC solution are obtained respectively； Meso is mixed into after placing meso -2,3- dimercaptosuccinic acid-WSC and liquaemin-WSC solution 1min-12h - DMSA/liquaemin-WSC solution, by the blood vessel branch of surface deposition plasma polyallylamine film Frame is soaked in reaction -48 hours 1 hour in the meso-DMSA/liquaemin-WSC solution, so Fully rinsed with PBS and distilled water respectively afterwards, dry, produce purpose thing.
The intravascular stent of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after intravascular stent implantation The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 9
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By artificial blood vessel (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, 3,3- dithiodipropionic acid and liquaemin are fixed altogether
3,3- dithiodipropionic acids are pressed into 0.01 μ g/ml-10 by 0.01 μ g/ml-10mg/ml and liquaemin Mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morpholines) 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml of ethyl sulfonic acid cushioning liquid, 1mg/ml N- hydroxysuccinimides, 3,3- dithiodipropionic acid-WSC and liquaemin-WSC solution are obtained respectively；Place 3,3- 3,3- dithiodipropionic acids/liquaemin is mixed into after dithiodipropionic acid-WSC and liquaemin-WSC solution 1min-12h - WSC solution, 3, the 3- bis- thio two is soaked in by the intravascular stent of surface deposition plasma polyallylamine film React -48 hours 1 hour, then fully rinsed with PBS and distilled water respectively in propionic acid/liquaemin-WSC solution, Dry, produce purpose thing.
The artificial blood vessel of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after artificial blood vessel is implanted into The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 10
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By artificial blood vessel (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, cystine and liquaemin are fixed altogether
Cystine is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains cystine-WSC and liquaemin-WSC solution respectively；Place cystine-WSC and liquaemin-WSC Cystine/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the cystine/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The artificial blood vessel of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after artificial blood vessel is implanted into The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 11
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By artificial blood vessel (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, lipoic acid and liquaemin are fixed altogether
Lipoic acid is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains lipoic acid-WSC and liquaemin-WSC solution respectively；Place lipoic acid-WSC and liquaemin-WSC Lipoic acid/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the lipoic acid/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The artificial blood vessel of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after artificial blood vessel is implanted into The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 12
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By artificial blood vessel (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, meso -2,3- dimercaptosuccinic acids and liquaemin are fixed altogether
Meso -2,3- dimercaptosuccinic acids are pressed into 0.01 μ g/ml by 0.01 μ g/ml-10mg/ml and liquaemin - 10mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morphines Quinoline) ethyl sulfonic acid cushioning liquid, 1mg/ml 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxysuccinimides, meso-DMSA-WSC and liquaemin-WSC solution are obtained respectively； Meso is mixed into after placing meso -2,3- dimercaptosuccinic acid-WSC and liquaemin-WSC solution 1min-12h - DMSA/liquaemin-WSC solution, by the blood vessel branch of surface deposition plasma polyallylamine film Frame is soaked in reaction -48 hours 1 hour in the meso-DMSA/liquaemin-WSC solution, so Fully rinsed with PBS and distilled water respectively afterwards, dry, produce purpose thing.
The artificial blood vessel of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after artificial blood vessel is implanted into The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 13
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, artificial blood vessel (PET, PTFE, PCL, PLGA, PLLA etc.) is soaked in dopamine/hexamethylene diamine with material 1-48h is reacted in solution, is then fully rinsed with distilled water, dries, produces.
B, 3,3- dithiodipropionic acid and liquaemin are fixed altogether
3,3- dithiodipropionic acids are pressed into 0.01 μ g/ml-10 by 0.01 μ g/ml-10mg/ml and liquaemin Mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morpholines) 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml of ethyl sulfonic acid cushioning liquid, 1mg/ml N- hydroxysuccinimides, 3,3- dithiodipropionic acid-WSC and liquaemin-WSC solution are obtained respectively；Place 3,3- 3,3- dithiodipropionic acids/liquaemin is mixed into after dithiodipropionic acid-WSC and liquaemin-WSC solution 1min-12h - WSC solution, 3, the 3- bis- thio two is soaked in by the intravascular stent of surface deposition plasma polyallylamine film React -48 hours 1 hour, then fully rinsed with PBS and distilled water respectively in propionic acid/liquaemin-WSC solution, Dry, produce purpose thing.
The artificial blood vessel of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after artificial blood vessel is implanted into The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 14
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, artificial blood vessel (PET, PTFE, PCL, PLGA, PLLA etc.) is soaked in dopamine/hexamethylene diamine with material 1-48h is reacted in solution, is then fully rinsed with distilled water, dries, produces.
B, cystine and liquaemin are fixed altogether
Cystine is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains cystine-WSC and liquaemin-WSC solution respectively；Place cystine-WSC and liquaemin-WSC Cystine/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the cystine/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The artificial blood vessel of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after artificial blood vessel is implanted into The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 15
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, artificial blood vessel (PET, PTFE, PCL, PLGA, PLLA etc.) is soaked in dopamine/hexamethylene diamine with material 1-48h is reacted in solution, is then fully rinsed with distilled water, dries, produces.
B, lipoic acid and liquaemin are fixed altogether
Lipoic acid is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains lipoic acid-WSC and liquaemin-WSC solution respectively；Place lipoic acid-WSC and liquaemin-WSC Lipoic acid/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the lipoic acid/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The artificial blood vessel of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after artificial blood vessel is implanted into The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 16
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, artificial blood vessel (PET, PTFE, PCL, PLGA, PLLA etc.) is soaked in dopamine/hexamethylene diamine with material 1-48h is reacted in solution, is then fully rinsed with distilled water, dries, produces.
B, meso -2,3- dimercaptosuccinic acids and liquaemin are fixed altogether
Meso -2,3- dimercaptosuccinic acids are pressed into 0.01 μ g/ml by 0.01 μ g/ml-10mg/ml and liquaemin - 10mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morphines Quinoline) ethyl sulfonic acid cushioning liquid, 1mg/ml 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxysuccinimides, meso-DMSA-WSC and liquaemin-WSC solution are obtained respectively； Meso is mixed into after placing meso -2,3- dimercaptosuccinic acid-WSC and liquaemin-WSC solution 1min-12h - DMSA/liquaemin-WSC solution, by the blood vessel branch of surface deposition plasma polyallylamine film Frame is soaked in reaction -48 hours 1 hour in the meso-DMSA/liquaemin-WSC solution, so Fully rinsed with PBS and distilled water respectively afterwards, dry, produce purpose thing.
The intravascular stent of the bionical natural endothelium functionalization not only has excellent anticoagulation, promotes inner skin cell viscosity to echo The adhesion of multiplication capacity and suppression smooth muscle cell and the ability of propagation, in addition, being persistently catalyzed after intravascular stent implantation The nitric oxide of generation has therapeutic action to atherosclerosis, so as to reach the vascular tissue for repairing lesion.
Embodiment 17
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By CVC (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, 3,3- dithiodipropionic acid and liquaemin are fixed altogether
3,3- dithiodipropionic acids are pressed into 0.01 μ g/ml-10 by 0.01 μ g/ml-10mg/ml and liquaemin Mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morpholines) 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml of ethyl sulfonic acid cushioning liquid, 1mg/ml N- hydroxysuccinimides, 3,3- dithiodipropionic acid-WSC and liquaemin-WSC solution are obtained respectively；Place 3,3- 3,3- dithiodipropionic acids/liquaemin is mixed into after dithiodipropionic acid-WSC and liquaemin-WSC solution 1min-12h - WSC solution, 3, the 3- bis- thio two is soaked in by the intravascular stent of surface deposition plasma polyallylamine film React -48 hours 1 hour, then fully rinsed with PBS and distilled water respectively in propionic acid/liquaemin-WSC solution, Dry, produce purpose thing.
The CVC of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 18
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By CVC (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, cystine and liquaemin are fixed altogether
Cystine is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains cystine-WSC and liquaemin-WSC solution respectively；Place cystine-WSC and liquaemin-WSC Cystine/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the cystine/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The CVC of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 19
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By CVC (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, lipoic acid and liquaemin are fixed altogether
Lipoic acid is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains lipoic acid-WSC and liquaemin-WSC solution respectively；Place lipoic acid-WSC and liquaemin-WSC Lipoic acid/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the lipoic acid/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The CVC of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 20
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By CVC (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, meso -2,3- dimercaptosuccinic acids and liquaemin are fixed altogether
Meso -2,3- dimercaptosuccinic acids are pressed into 0.01 μ g/ml by 0.01 μ g/ml-10mg/ml and liquaemin - 10mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morphines Quinoline) ethyl sulfonic acid cushioning liquid, 1mg/ml 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxysuccinimides, meso-DMSA-WSC and liquaemin-WSC solution are obtained respectively； Meso is mixed into after placing meso -2,3- dimercaptosuccinic acid-WSC and liquaemin-WSC solution 1min-12h - DMSA/liquaemin-WSC solution, by the blood vessel branch of surface deposition plasma polyallylamine film Frame is soaked in reaction -48 hours 1 hour in the meso-DMSA/liquaemin-WSC solution, so Fully rinsed with PBS and distilled water respectively afterwards, dry, produce purpose thing.
The CVC of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 21
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, by CVC (PET, PTFE, PCL, PLGA, PLLA etc.) be soaked in material dopamine/oneself two 1-48h is reacted in the solution of amine, is then fully rinsed with distilled water, dries, produces.
B, 3,3- dithiodipropionic acid and liquaemin are fixed altogether
3,3- dithiodipropionic acids are pressed into 0.01 μ g/ml-10 by 0.01 μ g/ml-10mg/ml and liquaemin Mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morpholines) 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml of ethyl sulfonic acid cushioning liquid, 1mg/ml N- hydroxysuccinimides, 3,3- dithiodipropionic acid-WSC and liquaemin-WSC solution are obtained respectively；Place 3,3- 3,3- dithiodipropionic acids/liquaemin is mixed into after dithiodipropionic acid-WSC and liquaemin-WSC solution 1min-12h - WSC solution, 3, the 3- bis- thio two is soaked in by the intravascular stent of surface deposition plasma polyallylamine film React -48 hours 1 hour, then fully rinsed with PBS and distilled water respectively in propionic acid/liquaemin-WSC solution, Dry, produce purpose thing.
The CVC of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 22
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, by CVC (PET, PTFE, PCL, PLGA, PLLA etc.) be soaked in material dopamine/oneself two 1-48h is reacted in the solution of amine, is then fully rinsed with distilled water, dries, produces.
B, cystine and liquaemin are fixed altogether
Cystine is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains cystine-WSC and liquaemin-WSC solution respectively；Place cystine-WSC and liquaemin-WSC Cystine/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the cystine/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The CVC of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 23
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, by CVC (PET, PTFE, PCL, PLGA, PLLA etc.) be soaked in material dopamine/oneself two 1-48h is reacted in the solution of amine, is then fully rinsed with distilled water, dries, produces.
B, lipoic acid and liquaemin are fixed altogether
Lipoic acid is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains lipoic acid-WSC and liquaemin-WSC solution respectively；Place lipoic acid-WSC and liquaemin-WSC Lipoic acid/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the lipoic acid/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The CVC of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 24
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, by CVC (PET, PTFE, PCL, PLGA, PLLA etc.) be soaked in material dopamine/oneself two 1-48h is reacted in the solution of amine, is then fully rinsed with distilled water, dries, produces.
B, meso -2,3- dimercaptosuccinic acids and liquaemin are fixed altogether
Meso -2,3- dimercaptosuccinic acids are pressed into 0.01 μ g/ml by 0.01 μ g/ml-10mg/ml and liquaemin - 10mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morphines Quinoline) ethyl sulfonic acid cushioning liquid, 1mg/ml 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxysuccinimides, meso-DMSA-WSC and liquaemin-WSC solution are obtained respectively； Meso is mixed into after placing meso -2,3- dimercaptosuccinic acid-WSC and liquaemin-WSC solution 1min-12h - DMSA/liquaemin-WSC solution, by the blood vessel branch of surface deposition plasma polyallylamine film Frame is soaked in reaction -48 hours 1 hour in the meso-DMSA/liquaemin-WSC solution, so Fully rinsed with PBS and distilled water respectively afterwards, dry, produce purpose thing.
The CVC of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 25
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By extracorporal circulatory system conduit (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, 3,3- dithiodipropionic acid and liquaemin are fixed altogether
3,3- dithiodipropionic acids are pressed into 0.01 μ g/ml-10 by 0.01 μ g/ml-10mg/ml and liquaemin Mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morpholines) 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml of ethyl sulfonic acid cushioning liquid, 1mg/ml N- hydroxysuccinimides, 3,3- dithiodipropionic acid-WSC and liquaemin-WSC solution are obtained respectively；Place 3,3- 3,3- dithiodipropionic acids/liquaemin is mixed into after dithiodipropionic acid-WSC and liquaemin-WSC solution 1min-12h - WSC solution, 3, the 3- bis- thio two is soaked in by the intravascular stent of surface deposition plasma polyallylamine film React -48 hours 1 hour, then fully rinsed with PBS and distilled water respectively in propionic acid/liquaemin-WSC solution, Dry, produce purpose thing.
The extracorporal circulatory system conduit of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 26
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By extracorporal circulatory system conduit (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, cystine and liquaemin are fixed altogether
Cystine is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains cystine-WSC and liquaemin-WSC solution respectively；Place cystine-WSC and liquaemin-WSC Cystine/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the cystine/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The extracorporal circulatory system conduit of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 27
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By extracorporal circulatory system conduit (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, lipoic acid and liquaemin are fixed altogether
Lipoic acid is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains lipoic acid-WSC and liquaemin-WSC solution respectively；Place lipoic acid-WSC and liquaemin-WSC Lipoic acid/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the lipoic acid/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The extracorporal circulatory system conduit of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 28
A kind of preparation method with endothelium bionic function coating, its step is：
A, plasma polyallylamine film deposition
Surface is rich in the plasma polymerization film preparation of amido：By extracorporal circulatory system conduit (PET, PTFE, PCL, PLGA, PLLA etc.) it is put into film deposition chamber, when reative cell vacuum is evacuated to as 0.01-2Pa, being passed through flow is 0.5-5sccm argon gas is as discharge gas, and be passed through makes operating pressure be 1-10 as the allylamine of reacting gas Pa, when it is to enter behavior under conditions of 5-100% that radio-frequency power 5-10W, back bias voltage, which are 0-150V, pulse duty factor, The plasma polyallylamine thin film deposition of 5-60 minutes, i.e., on intravascular stent surface deposition rich in amino grade from Daughter polyallylamine film.
B, meso -2,3- dimercaptosuccinic acids and liquaemin are fixed altogether
Meso -2,3- dimercaptosuccinic acids are pressed into 0.01 μ g/ml by 0.01 μ g/ml-10mg/ml and liquaemin - 10mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morphines Quinoline) ethyl sulfonic acid cushioning liquid, 1mg/ml 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxysuccinimides, meso-DMSA-WSC and liquaemin-WSC solution are obtained respectively； Meso is mixed into after placing meso -2,3- dimercaptosuccinic acid-WSC and liquaemin-WSC solution 1min-12h - DMSA/liquaemin-WSC solution, by the blood vessel branch of surface deposition plasma polyallylamine film Frame is soaked in reaction -48 hours 1 hour in the meso-DMSA/liquaemin-WSC solution, so Fully rinsed with PBS and distilled water respectively afterwards, dry, produce purpose thing.
The extracorporal circulatory system conduit of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 29
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, by extracorporal circulatory system conduit (PET, PTFE, PCL, PLGA, PLLA etc.) be soaked in material dopamine/oneself two 1-48h is reacted in the solution of amine, is then fully rinsed with distilled water, dries, produces.
B, 3,3- dithiodipropionic acid and liquaemin are fixed altogether
3,3- dithiodipropionic acids are pressed into 0.01 μ g/ml-10 by 0.01 μ g/ml-10mg/ml and liquaemin Mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morpholines) 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml of ethyl sulfonic acid cushioning liquid, 1mg/ml N- hydroxysuccinimides, 3,3- dithiodipropionic acid-WSC and liquaemin-WSC solution are obtained respectively；Place 3,3- 3,3- dithiodipropionic acids/liquaemin is mixed into after dithiodipropionic acid-WSC and liquaemin-WSC solution 1min-12h - WSC solution, 3, the 3- bis- thio two is soaked in by the intravascular stent of surface deposition plasma polyallylamine film React -48 hours 1 hour, then fully rinsed with PBS and distilled water respectively in propionic acid/liquaemin-WSC solution, Dry, produce purpose thing.
The extracorporal circulatory system conduit of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 30
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, by extracorporal circulatory system conduit (PET, PTFE, PCL, PLGA, PLLA etc.) be soaked in material dopamine/oneself two 1-48h is reacted in the solution of amine, is then fully rinsed with distilled water, dries, produces.
B, cystine and liquaemin are fixed altogether
Cystine is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains cystine-WSC and liquaemin-WSC solution respectively；Place cystine-WSC and liquaemin-WSC Cystine/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the cystine/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The extracorporal circulatory system conduit of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 31
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, by extracorporal circulatory system conduit (PET, PTFE, PCL, PLGA, PLLA etc.) be soaked in material dopamine/oneself two 1-48h is reacted in the solution of amine, is then fully rinsed with distilled water, dries, produces.
B, lipoic acid and liquaemin are fixed altogether
Lipoic acid is distinguished by 0.01 μ g/ml-10mg/ml and liquaemin by 0.01 μ g/ml-10mg/ml sodium It is dissolved in WSC solution；The composition of WSC solution is molten for 9.76mg/ml 2- (N- morpholines) ethyl sulfonic acid buffering 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxyl ambers of liquid, 1mg/ml Amber acid amides, obtains lipoic acid-WSC and liquaemin-WSC solution respectively；Place lipoic acid-WSC and liquaemin-WSC Lipoic acid/liquaemin-WSC solution is mixed into after solution 1min-12h, surface deposition plasma polyallylamine is thin The intravascular stent of film, which is soaked in the lipoic acid/liquaemin-WSC solution, to react -48 hours 1 hour, then distinguishes Fully rinsed with PBS and distilled water, dry, produce purpose thing.
The extracorporal circulatory system conduit of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.
Embodiment 32
A kind of preparation method with endothelium bionic function coating, its step is：
A, Polyphenols and the copolymerization of polyamines cardinal extremity molecule prepare rich amido coating
0-20mg/ml dopamines and 0-40mg/ml hexamethylene diamines are dissolved in pH=8.5Tris-base buffer In, by extracorporal circulatory system conduit (PET, PTFE, PCL, PLGA, PLLA etc.) be soaked in material dopamine/oneself two 1-48h is reacted in the solution of amine, is then fully rinsed with distilled water, dries, produces.
B, meso -2,3- dimercaptosuccinic acids and liquaemin are fixed altogether
Meso -2,3- dimercaptosuccinic acids are pressed into 0.01 μ g/ml by 0.01 μ g/ml-10mg/ml and liquaemin - 10mg/ml sodium is dissolved separately in WSC solution；The composition of WSC solution is 9.76mg/ml 2- (N- morphines Quinoline) ethyl sulfonic acid cushioning liquid, 1mg/ml 1- ethyls -3- (3- dimethylamino-propyls) carbodiimides and 0.24mg/ml N- hydroxysuccinimides, meso-DMSA-WSC and liquaemin-WSC solution are obtained respectively； Meso is mixed into after placing meso -2,3- dimercaptosuccinic acid-WSC and liquaemin-WSC solution 1min-12h - DMSA/liquaemin-WSC solution, by the blood vessel branch of surface deposition plasma polyallylamine film Frame is soaked in reaction -48 hours 1 hour in the meso-DMSA/liquaemin-WSC solution, so Fully rinsed with PBS and distilled water respectively afterwards, dry, produce purpose thing.
The extracorporal circulatory system conduit of the bionical natural endothelium functionalization continues after not only having excellent long-term anticoagulation, implantation Being catalyzed the nitric oxide that produces can also effective antibacterial, the generation of the symptom such as reduction postoperative infection.

Claims (9)

1. one kind has endothelium bionic function coating, it is characterised in that fix tool simultaneously in biomaterial equipment surfaces There are the compound of disulfide bond containing carboxyl and heparin of nitric oxide catalytic activity.
2. according to claim 1 have endothelium bionic function coating, it is characterised in that the biomaterial Including but not limited to it is used for the biomaterial of cardiovascular implant material, including：Medical metal sill, inorganic material, High polymer material and degradable surface modified polymer material.
3. according to claim 1 have endothelium bionic function coating, it is characterised in that the medical metal Sill includes：Fe and its alloy, mg-based material, 316L SS, Ti, Ni-Ti alloy and CoCr alloy.
4. according to claim 1 have endothelium bionic function coating, it is characterised in that the inorganic material Including：Ti-O, TiN, pyrolytic carbon, aluminum oxide, hydroxyapatite, calcium phosphate.
5. according to claim 1 have endothelium bionic function coating, it is characterised in that the macromolecule material Material includes：PET、PTFE、PDMS.
6. according to claim 1 have endothelium bionic function coating, it is characterised in that described degradable Surface modified polymer material includes：PLA、PLGA、PCL、PTMC.
7. a kind of preparation method with endothelium bionic function coating, it is characterised in that：There to be nitric oxide catalysis The compound of disulfide bond containing carboxyl of activity is fixed on the biomaterial surface rich in amido, including its following step altogether with heparin Suddenly it is：
A, amine-containing functional films preparation
In coating of the biomaterial surface formation rich in amido of including but not limited to cardiovascular implant material；
B, the compound of disulfide bond containing carboxyl and heparin be fixed on the biomaterial surface rich in amido altogether, A is made Surface rich in amido coating biomaterial be soaked in the compound of disulfide bond containing carboxyl/heparin-WSC solution react, Then fully rinsed with buffer solution and distilled water respectively, target is made after drying has endothelium bionic function coating.
8. according to the method described in claim 1, it is characterised in that coating, which is made, in the step A includes but do not limit In following form：Plasma polymerized coating, Polyphenols and polyamines cardinal extremity molecule copolymerization coating, Electrostatic Absorption rich amine base Molecular coatings, the silane coupled terminal modified coating of amido.
9. according to the method described in claim 1, it is characterised in that the compound of disulfide bond containing carboxyl include but It is not limited to material：3,3- dithiodipropionic acids, cystine, lipoic acid, meso-DMSA and heparin Sodium and it is dissolved in WSC solution, WSC reaction solutions composition is 2- (N- morpholines) ethyl sulfonic acid (MES), 1- Ethyl -3- (3- dimethylamino-propyls) carbodiimide (EDC) and N- hydroxysuccinimides (NHS).
CN201610172970.9A 2016-03-23 2016-03-23 One kind has endothelium bionic function coating and preparation method thereof Pending CN107224621A (en)

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