CN107223849A - A kind of sorbic acid-Tea Polyphenols composite nanoparticle and its application - Google Patents
A kind of sorbic acid-Tea Polyphenols composite nanoparticle and its application Download PDFInfo
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- CN107223849A CN107223849A CN201610165457.7A CN201610165457A CN107223849A CN 107223849 A CN107223849 A CN 107223849A CN 201610165457 A CN201610165457 A CN 201610165457A CN 107223849 A CN107223849 A CN 107223849A
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- Prior art keywords
- composite nanoparticle
- sorbic acid
- tea polyphenols
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- composite
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3499—Organic compounds containing oxygen with doubly-bound oxygen
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3508—Organic compounds containing oxygen containing carboxyl groups
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
- A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
- A61K9/145—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with organic compounds
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A kind of sorbic acid Tea Polyphenols composite nanoparticle and its application, the present invention relates to nano-complex particle synthesis technical field；Its preparation method is as follows：First, composite nanoparticle is prepared；2nd, the envelop rate and drugloading rate of composite nanoparticle are detected, functional response performance test was carried out to the composite nanoparticle prepared, qualified composite nanoparticle is obtained；3rd, security performance detection is carried out to composite nanoparticle：From the overall security of composite nanoparticle, tested by hemolytic, vitro cytotoxicity test and acute toxicity test in mice study the inside and outside toxicity of composite nanoparticle, tentatively understand the intensity and property of its toxicity, obtain dose-effect relationship；The 4th, composite nanoparticle is applied to the exploitation of fresh-keeping and healthcare.Composite nanoparticle security performance is guaranteed, and important leverage is provided for later stage application, and is had a wide range of application, and a kind of new opplication is provided for existing fresh-keeping and healthcare field.
Description
Technical field
The present invention relates to nano-complex particle synthesis technical field, and in particular to a kind of sorbic acid-Tea Polyphenols is multiple
Close nano-particle and its application.
Background technology
Nano-carrier (phytosterol, gelatin, chitosan, lycopene) is special by its small-molecule chemical
Levy in addition to possessing the feature of medicine in itself, it is also special with good characterization of adsorption, slow release characteristic, dissolving
Property and targeting characteristic, the functional products such as sorbic acid-Tea Polyphenols are supported on nano-carrier surface, can be made
The bioactivity of product is strengthened, and realizes the targeted delivery of active material.Project is related to a variety of nanometers
The controlledly synthesis and modification of carrier, and filter out the nano-carrier that load capacity is strong, bio-compatibility is good.
The growth of sorbic acid and its esters to mould and yeast has good inhibiting effect, as natural anti-
Rotten agent is commonly used.But the fungistatic effect of sorbic acid is mainly determined by its acid molecule not dissociated.It is acid
Bigger, the fungistatic effect of sorbate molecule is better, and in the basic conditions, the fungistatic effect of sorbic acid compared with
Difference.Due to sorbic acid, solubility is smaller in water, and the performance of its bacteriostasis is limited by pH,
These all limit direct application of the sorbic acid in food.Nano-particle can deliver various types of things
Matter, improves the solubility of insoluble drug, and this is due to that the solubility of insoluble drug has with specific surface area
Close.
It is well known that Tea Polyphenols has antioxidation, have pre- to each stage of kinds of tumors formation
Anti- and inhibitory action, can absorb radioactive substance, is referred to as natural ultraviolet filter.Tea Polyphenols has
There is the stronger blood pressure that suppresses and change related enzyme activity, thus reduction can be played or keep blood pressure steady
Fixed effect.However, the less stable of Tea Polyphenols powder, under the conditions of humidity, sunlight, high temperature etc.
Easily occur the reactions such as oxidation, polymerization, condensation.Catechin isoreactivity material in Tea Polyphenols is in non-protected
Bioavailability is not high under state, when being taken directly as health medicine, can meet with by gastrointestinal disturbances structure
To destruction, its health-care effect have impact on.
Whether the particulate of nanoscale produces toxicity to the cell in living organism and organizer official rank, is it
Later stage can apply it is particularly critical and important the problem of.
The content of the invention
In view of the defects and deficiencies of the prior art, the present invention intends to provide a kind of mountain reasonable in design
Pears acid-Tea Polyphenols composite nanoparticle and its application, composite nanoparticle security performance is guaranteed, after being
Phase application provides important leverage, and has a wide range of application, and a kind of new opplication is provided for existing fresh-keeping and healthcare field.
To achieve the above object, the technical solution adopted by the present invention is：Its preparation method is as follows：
First, by sorbic acid and Tea Polyphenols using emulsion polymerization, molecular self-assembling method, matrix polymerization method or
Person's emulsion dispersion method is synthesized with nano-carrier, prepares composite nanoparticle；
2nd, the envelop rate and drugloading rate of composite nanoparticle are detected, to the composite nanoparticle prepared
Functional response performance test was carried out, qualified composite nanoparticle is obtained；
3rd, security performance detection is carried out to composite nanoparticle：From the overall security of composite nanoparticle
Set out, tested by hemolytic, vitro cytotoxicity test and acute toxicity test in mice research are combined and received
The inside and outside toxicity of rice corpuscles, tentatively understands the intensity and property of its toxicity, obtains dose-effect relationship；
The 4th, composite nanoparticle is applied to the exploitation of fresh-keeping and healthcare：Pass through microbiological indicator, thio bar
Than appropriate acid number, the antiseptic property of the measure analysis composite nanoparticle of VBN and to beverage product
The influence of matter, is at the same time combined and is received by Cell culture invitro and animal experiment research sorbic acid-Tea Polyphenols
The antitumor activity of rice corpuscles, utilizes form of the means such as inverted microscope, TEM, SEM to tumour cell
Structure is characterized, apoptosis rate and analysis cell cycle using flow cytomery cell, inquires into multiple
The mechanism of action of nano-particle is closed, is the functional food or medicine of sorbic acid-Tea Polyphenols composite nanoparticle
Applicating and exploitation provide theoretical foundation.
The present invention has the beneficial effect that：A kind of sorbic acid-Tea Polyphenols composite nanoparticle of the present invention and its
Using composite nanoparticle security performance is guaranteed, and important leverage, and application are provided for later stage application
Scope is wide, and a kind of new opplication is provided for existing fresh-keeping and healthcare field, and the present invention has simple in construction, setting
Rationally, the advantages of low manufacture cost.
Embodiment
Present embodiment use technical scheme be：Its preparation method is as follows：
First, by sorbic acid and Tea Polyphenols using emulsion polymerization, molecular self-assembling method, matrix polymerization method or
Person's emulsion dispersion method is synthesized with nano-carrier, prepares composite nanoparticle；
2nd, the envelop rate and drugloading rate of composite nanoparticle are detected, to the composite nanoparticle prepared
Carry out function phase and answer performance test, and obtain qualified composite nanoparticle；
3rd, security performance detection is carried out to composite nanoparticle：From the overall security of composite nanoparticle
Set out, tested by hemolytic, vitro cytotoxicity test and acute toxicity test in mice research are combined and received
The inside and outside toxicity of rice corpuscles, tentatively understands the intensity and property of its toxicity, obtains dose-effect relationship；
The 4th, composite nanoparticle is applied to the fresh-keeping and healthcare of beverage：Pass through microbiological indicator, thio bar
Than appropriate acid number, the antiseptic property of the measure analysis composite nanoparticle of VBN and to beverage product
The influence of matter, is at the same time combined and is received by Cell culture invitro and animal experiment research sorbic acid-Tea Polyphenols
The antitumor activity of rice corpuscles, utilizes form of the means such as inverted microscope, TEM, SEM to tumour cell
Structure is characterized, apoptosis rate and analysis cell cycle using flow cytomery cell, inquires into multiple
The mechanism of action of nano-particle is closed, is the functional food or medicine of sorbic acid-Tea Polyphenols composite nanoparticle
Applicating and exploitation provide theoretical foundation.
Present embodiment has the beneficial effect that：A kind of sorbic acid-Tea Polyphenols described in present embodiment
Composite nanoparticle and its application, composite nanoparticle security performance are guaranteed, and are provided for later stage application
Important leverage, and have a wide range of application, provide a kind of new opplication, present invention tool for existing fresh-keeping and healthcare field
Have simple in construction, it is reasonable to set, the advantages of low manufacture cost.
It is described above, it is merely illustrative of the technical solution of the present invention and unrestricted, ordinary skill people
Other modifications or equivalent substitution that member is made to technical scheme, without departing from skill of the present invention
The spirit and scope of art scheme, all should cover among scope of the presently claimed invention.
Claims (1)
1. a kind of sorbic acid-Tea Polyphenols composite nanoparticle and its application, it is characterised in that：Its preparation
Method is as follows：
(1) sorbic acid and Tea Polyphenols, are used into emulsion polymerization, molecular self-assembling method, matrix polymerization method
Or emulsion dispersion method is synthesized with nano-carrier, prepares composite nanoparticle；
(2) envelop rate and drugloading rate of composite nanoparticle, are detected, to the composite nano-granule prepared
Son carried out functional response performance test, obtained qualified composite nanoparticle；
(3) security performance detection, is carried out to composite nanoparticle：From the general safety of composite nanoparticle
Property set out, tested by hemolytic, vitro cytotoxicity test and acute toxicity test in mice research it is compound
The inside and outside toxicity of nano-particle, tentatively understands the intensity and property of its toxicity, obtains dose-effect relationship；
(4), composite nanoparticle is applied to the exploitation of fresh-keeping and healthcare：By microbiological indicator, thio
Barbital acid number, the measure of VBN analyze the antiseptic property of composite nanoparticle and to beverage
The influence of quality, at the same time studies sorbic acid-Tea Polyphenols by Cell culture invitro and animal experiment and is combined
The antitumor activity of nano-particle, utilizes shape of the means such as inverted microscope, TEM, SEM to tumour cell
State structure is characterized, apoptosis rate and analysis cell cycle using flow cytomery cell, is inquired into
The mechanism of action of composite nanoparticle, is the functional food or medicine of sorbic acid-Tea Polyphenols composite nanoparticle
The applicating and exploitation of product provides theoretical foundation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610165457.7A CN107223849A (en) | 2016-03-23 | 2016-03-23 | A kind of sorbic acid-Tea Polyphenols composite nanoparticle and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610165457.7A CN107223849A (en) | 2016-03-23 | 2016-03-23 | A kind of sorbic acid-Tea Polyphenols composite nanoparticle and its application |
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| Publication Number | Publication Date |
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| CN107223849A true CN107223849A (en) | 2017-10-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610165457.7A Pending CN107223849A (en) | 2016-03-23 | 2016-03-23 | A kind of sorbic acid-Tea Polyphenols composite nanoparticle and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107737043A (en) * | 2017-11-24 | 2018-02-27 | 中华全国供销合作总社杭州茶叶研究所 | A kind of self-assembled nanometer compound, preparation method and application that Tea Polyphenols is loaded based on hyaluronic acid |
-
2016
- 2016-03-23 CN CN201610165457.7A patent/CN107223849A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107737043A (en) * | 2017-11-24 | 2018-02-27 | 中华全国供销合作总社杭州茶叶研究所 | A kind of self-assembled nanometer compound, preparation method and application that Tea Polyphenols is loaded based on hyaluronic acid |
| CN107737043B (en) * | 2017-11-24 | 2020-09-18 | 中华全国供销合作总社杭州茶叶研究所 | Hyaluronic acid-based tea polyphenol-loaded self-assembled nano-composite, and preparation method and application thereof |
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| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171003 |
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| WD01 | Invention patent application deemed withdrawn after publication |