CN107219289A - A kind of isoelectric focusing electrophoresis instrument - Google Patents
A kind of isoelectric focusing electrophoresis instrument Download PDFInfo
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- CN107219289A CN107219289A CN201710341396.XA CN201710341396A CN107219289A CN 107219289 A CN107219289 A CN 107219289A CN 201710341396 A CN201710341396 A CN 201710341396A CN 107219289 A CN107219289 A CN 107219289A
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- Prior art keywords
- groove
- isoelectric focusing
- focusing
- electrophoresis instrument
- instrument
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44795—Isoelectric focusing
Abstract
A kind of isoelectric focusing electrophoresis instrument, positive electrode electrode end system, negative electrode electrodes end system, focusing groove, by way of running multiple macroblock modes simultaneously and focusing on groove, single monoblock focusing groove, electrophoresis apparatus is run adhesive tape amount and reach 13~36, greatly improve operational efficiency, substantial amounts of material can lack isolating and purifying for batch as far as possible, with reduce it is multiple batches of between various factors change the resultant error brought, improve stability, the efficiency isolated and purified is improved simultaneously, shorten the cycle of operation, reduce cost, save the input of human and material resources.
Description
Technical field
The present invention relates to biotechnology separation detection apparatus field, specially protein isoelectric focusing electrophoresis equipment.
Background technology
At present, albumen Quality Research is the study hotspot of rear era gene.In the research to Separation of Proteins and enrichment,
Two dimensional gel electrophore- sis (Two-dimensional electrophoresis, 2DE) is a use very universal classical skill
Art.In this technology, electrophoresis is an important link for isoelectric focusing (isoelectric focusing, IEF), and is used
The progress experiment of 7cm adhesive tape focusing electrophoresis is groped and medical sample detection is the more commonly used and easy method.
There are a variety of isoelectric focusing electrophoresis instrument that groove is focused on equipped with 7cm adhesive tape on Vehicles Collected from Market, but at most once can only be complete
Run simultaneously into 12 adhesive tape, detected for several samples, can only repeatedly be focused experiment.Temperature, electric current, electrophoretic medium etc.
Multiple factors can all produce influence to testing result, it is multiple batches of between the parameter value of various factors have different, stability
It is relatively low, be not suitable for large-scale experiment grope and batch medical sample detection.
In order to solve the above-mentioned technical problem, the present invention, which proposes a kind of isoelectric focusing electrophoresis instrument, is used for high-volume Protein Detection
Application method, up to 36 adhesive tape focusing electrophoresis can be carried out simultaneously, far above 12 channel patterns used at present, and are grasped
Make simple, reduce difference between the batch that test of many times is caused due to reasons such as multifactor parameter variations, stability, focus detection essence
Degree is significantly improved；Human and material resources, financial resources are also greatlyd save simultaneously, and the total process of isoelectric focusing shortens, cost reduction.
The content of the invention
A kind of isoelectric focusing electrophoresis instrument, including：Positive electrode electrode end system, negative electrode electrodes end system, focusing groove, it is described
Positive and negative electrode electrode end system is the contact device between instrument high voltage power supply and experiment adhesive tape, focuses on groove and uses passage electrophoresis,
Focusing groove wherein in isoelectric focusing electrophoresis instrument is provided with 13~36 adhesive tape.
Focusing groove in currently marketed isoelectric focusing electrophoresis instrument once can be only done 12 provided with 1~12 adhesive tape
Adhesive tape is run simultaneously, for several samples or large batch of sample detection, can only carry out multi-focusing experiment.Due to multiple factors
Influence will be produced on testing result, multiple batches of operation have different, stability poor, be not suitable for testing on a large scale grope with
And large batch of medical sample detection.Above-mentioned electrofocusing meter flexible can be adjusted according to the how much of the sample type and batch that need to be detected
The quantity of adhesive tape is saved, when sample batch is larger or needs separated in synchronization to purify several samples, by increasing isoelectric focusing electrophoresis instrument
The quantity of middle adhesive tape can reduce focusing batch, or even disposable focus on completes, and be prevented effectively from the multiple batches of experiment mistake for operating and bringing
Difference and unstability, while can shorten focal time, reduce material resources, the input of manpower, reduction focuses on experimental cost.
It is preferred that, the isoelectric focusing electrophoresis instrument includes >=2 macroblock modes and focuses on groove.All macroblock modes are focused on into groove
It is put on the temperature control plate in electrophoresis tank, all macroblock modes focusing groove is plugged into positive and negative electrode electrode end system, each electrode tip
System connects instrument high voltage power supply respectively, it is ensured that the parameter value such as temperature, voltage, electric current during isoelectric focusing is identical.When need
When the albumen quality of separation is more, albumen as much as possible can be disposably handled by increasing the quantity of macroblock mode focusing groove
Matter, reduces the batch of protein processing, reduces experimental error, and follow-up detection error is completed to reduce separation as far as possible.
It is preferred that, each macroblock mode focuses on groove provided with 12 focus channels.Port number in the focusing groove of in the market
Amount is commonly 12, during large batch of protein electrophorese operation, 2 or 3 piecemeals directly can be focused on into groove and be put into electrophoresis tank together
It is middle to carry out with the detection of batch Separation of Proteins, without in order to reduce the focusing groove that electrophoresis batch remakes more multichannel, operating
Get up more convenient.
It is preferred that, 1 monoblock pattern is placed in the isoelectric focusing electrophoresis instrument and focuses on groove, wherein monoblock pattern focusing groove is
Multiple macroblock modes focus on the integrally formed integral structure of groove.The monoblock pattern of integration, which focuses on groove, can meet large batch of
Protein completes separation detection with batch or less batch, shortens focal time and optimizes focusing effect.
It is preferred that, the monoblock pattern focuses on groove provided with 24 or 36 focus channels.Such as Fig. 2,24 focus channels it is poly-
Burnt groove is the overall structure of 2 macroblock mode integrations；Such as Fig. 3, the focusing groove of 36 focus channels is 3 macroblock mode one
The overall structure of change.
It is preferred that, the length of the adhesive tape is 7cm~24cm.
It is preferred that, the isoelectric focusing electrophoresis instrument is used for the isoelectric focusing of protein or the dielectrophoresis of protein.Albumen
The processing of matter be divided into first to isoelectric focusing, second to protein molecular weight separate.Either only have first to wait electricity
Focus on, or first and second to sequential focusing separate, above-mentioned isoelectric focusing electrophoresis instrument can less batch completion, reality
Test result stable, the cycle of operation shortens, cost reduction.
It is preferred that, groove is focused in the isoelectric focusing electrophoresis instrument and is applied to horizontal focusing groove, protein is isolated and purified, with
And multiple macroblock modes are run or individually the operation of monoblock mode electrophoretic is used simultaneously.
Brief description of the drawings：
Embodiment is further described below in conjunction with the accompanying drawings, wherein：
Fig. 1 is that present invention specific implementation case 1 focuses on groove schematic diagram.
Fig. 2 is that present invention specific implementation case 2 focuses on groove schematic diagram.
Fig. 3 is that present invention specific implementation case 3 focuses on groove schematic diagram.
Primary structure number explanation
Following embodiment will further illustrate the present invention with reference to above-mentioned accompanying drawing.
Case 1 is embodied：
Such as Fig. 1, a kind of high-volume Protein Detection for isoelectric focusing electrophoresis focuses on slot device, including：Positive electrode electrode
End system 1, negative electrode electrodes end system 2 and macroblock mode focus on groove 3.Macroblock mode is that independent fritter focuses on groove, is gathered per fritter
Burnt groove have can be put into simultaneously in 12 focus channels, instrument and equipment 3 independent block patterns focus on groove 3, i.e. macroblock modes can
36 adhesive tape focusing electrophoresis are carried out simultaneously.Positive and negative electrode end system is that the contact between instrument high voltage power supply and experiment adhesive tape is filled
Put.It is put in use, 3 sectional types are focused on into groove on the temperature control plate of instrument and equipment, buckles positive and negative electrode end system respectively afterwards
System, adds corresponding reagent solution, sets required voltage electric current, electrophoresis can be started by plugging in.The isoelectric focusing electrophoresis
Instrument reduces the electrophoresis batch of protein, improves the otherness between electrophoresis result stability, reduction batch.
Case 2 is embodied：
Illustrate present embodiment with reference to Fig. 2, Fig. 2 is that monoblock pattern focuses on groove, including：Positive electrode electrode end system, negative electricity
Pole electrode end system and monoblock pattern focus on groove.Focus on groove and use passage electrophoresis, monoblock pattern is that a monoblock pattern focuses on groove,
Inside there are 36 focus channels, 36 adhesive tape focusing electrophoresis can be run simultaneously.In use, focusing groove to be put in the temperature of instrument and equipment
Control on plate, for monoblock pattern, one piece of focusing groove can be placed, 36 adhesive tape can be at most put into while being focused electrophoresis.Respectively
Install after positive and negative electrode end system, add corresponding reagent solution, set required voltage electric current, plugging in start
Electrophoresis.Focusing is finished, successively data storage, is shut power supply and is taken out the adhesive tape progress subsequent experimental focused on.
Case 3 is embodied：
Present embodiment is as different from Example 1：A kind of described high-volume albumen for isoelectric focusing electrophoresis is examined
Survey to focus on and can be put into 2 independent block patterns focusing grooves 3 in slot device simultaneously, while running 24 adhesive tape.Other and embodiment 1
It is identical.Present embodiment is used for dielectrophoresis, improves dielectrophoresis material and isolates and purifies efficiency and stability.
Case 4 is embodied：
Present embodiment is as different from Example 1：A kind of described high-volume albumen for isoelectric focusing electrophoresis is examined
Survey to focus on and can be put into 1 independent block pattern focusing groove 3 in slot device simultaneously.It is other same as Example 1.Present embodiment is used
The dielectrophoresis isolated and purified in a small amount of protein or nucleic acid.
Embodiment described above only expresses the several embodiments of the present invention, and it describes more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (8)
1. a kind of isoelectric focusing electrophoresis instrument, including：Positive electrode electrode end system, negative electrode electrodes end system, focusing groove, it is described
Positive and negative electrode end system is the contact device between instrument high voltage power supply and experiment adhesive tape, focuses on groove and uses passage electrophoresis, it is special
Levy and be：Focusing groove in the isoelectric focusing electrophoresis instrument is provided with 13~36 adhesive tape.
2. isoelectric focusing electrophoresis instrument as claimed in claim 1, it is characterised in that：Focus on groove and focus on groove for the macroblock mode of >=2.
3. isoelectric focusing electrophoresis instrument as claimed in claim 2, it is characterised in that：The macroblock mode focuses on groove provided with 12 focusing
Passage.
4. isoelectric focusing electrophoresis instrument as claimed in claim 1, it is characterised in that：It is that monoblock pattern focuses on groove to focus on groove.
5. isoelectric focusing electrophoresis instrument as claimed in claim 4, it is characterised in that：The monoblock pattern focuses on groove by multiple piecemeal moulds
Formula focuses on groove integrated molding structure, provided with 24 or 36 focus channels.
6. isoelectric focusing electrophoresis instrument as claimed in claim 1, it is characterised in that：The length of the adhesive tape is 7cm~24cm.
7. isoelectric focusing electrophoresis instrument as claimed in claim 1, it is characterised in that：The isoelectric focusing electrophoresis instrument be used for sample etc.
Electrofocusing or dielectrophoresis.
8. the isoelectric focusing electrophoresis instrument as described in any in claim 1~7, it is characterised in that：In the isoelectric focusing electrophoresis instrument
Focus on groove and be applied to vertical focusing groove or horizontal focusing groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710341396.XA CN107219289A (en) | 2017-05-16 | 2017-05-16 | A kind of isoelectric focusing electrophoresis instrument |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710341396.XA CN107219289A (en) | 2017-05-16 | 2017-05-16 | A kind of isoelectric focusing electrophoresis instrument |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999010735A1 (en) * | 1997-08-28 | 1999-03-04 | Caliper Technologies Corporation | Improved controller/detector interfaces for microfluidic systems |
| US20060049051A1 (en) * | 2004-09-03 | 2006-03-09 | Combisep, Inc. | Microfabricated chip and method of use |
| US7517442B1 (en) * | 1999-08-09 | 2009-04-14 | Life Technologies Corporation | Facile method and apparatus for the analysis of biological macromolecules in two dimensions using common and familiar electrophoresis formats |
| CN104788536A (en) * | 2015-04-28 | 2015-07-22 | 扬州大学 | Testing device for isoelectric focusing separation of amphoteric electrolyte and application method of testing device |
| EP2411405B1 (en) * | 2009-03-25 | 2015-09-09 | Bio-Rad Laboratories, Inc. | Isoelectric focusing tray and electrode assembly for alternate gel strip orientations |
| CN105466994A (en) * | 2015-12-30 | 2016-04-06 | 中国计量学院 | Electrophoresis tank device for isoelectric focusing electrophoresis |
-
2017
- 2017-05-16 CN CN201710341396.XA patent/CN107219289A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999010735A1 (en) * | 1997-08-28 | 1999-03-04 | Caliper Technologies Corporation | Improved controller/detector interfaces for microfluidic systems |
| US7517442B1 (en) * | 1999-08-09 | 2009-04-14 | Life Technologies Corporation | Facile method and apparatus for the analysis of biological macromolecules in two dimensions using common and familiar electrophoresis formats |
| US20060049051A1 (en) * | 2004-09-03 | 2006-03-09 | Combisep, Inc. | Microfabricated chip and method of use |
| EP2411405B1 (en) * | 2009-03-25 | 2015-09-09 | Bio-Rad Laboratories, Inc. | Isoelectric focusing tray and electrode assembly for alternate gel strip orientations |
| CN104788536A (en) * | 2015-04-28 | 2015-07-22 | 扬州大学 | Testing device for isoelectric focusing separation of amphoteric electrolyte and application method of testing device |
| CN105466994A (en) * | 2015-12-30 | 2016-04-06 | 中国计量学院 | Electrophoresis tank device for isoelectric focusing electrophoresis |
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Application publication date: 20170929 |