CN107216201B - Preparation method of medium for cultivating sorrel straw of lentinus edodes - Google Patents
Preparation method of medium for cultivating sorrel straw of lentinus edodes Download PDFInfo
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- CN107216201B CN107216201B CN201710497457.1A CN201710497457A CN107216201B CN 107216201 B CN107216201 B CN 107216201B CN 201710497457 A CN201710497457 A CN 201710497457A CN 107216201 B CN107216201 B CN 107216201B
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- C05—FERTILISERS; MANUFACTURE THEREOF
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Abstract
The invention provides a preparation method of an Indian pyricularia indica straw culture medium for cultivating mushrooms, which comprises the steps of (1) inoculating Indian pyricularia indica in a Kafer culture medium, carrying out shaking culture at the rotating speed of 120-150 r/min at the temperature of 26-30 ℃ for 6-8 d, sampling and measuring the number of viable bacteria after the culture is finished, and dissolving the culture in distilled water to ensure that the number of the viable bacteria in each milliliter of solution is 1 × 1062, the number of the bacteria obtained in the step 1 is 1 × 106And (3) spraying the bacterial liquid per mL onto the surface of a straw culture medium according to the proportion of 1-15% of the inoculation amount, and fermenting at 25-37 ℃ for 12-24 h to obtain the Indian pear spore straw culture medium for cultivating the lentinus edodes. According to the invention, the Piriformospora indica is added, and the optimal cultivation conditions are selected, so that the growth of the root filaments of the shiitake mushrooms can be promoted, and the yield of the shiitake mushrooms is further improved.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a preparation method of a pyricularia indica straw culture medium for cultivating mushrooms.
Background
The cultivation of the mushrooms is originated from China and has been over 800 years old so far. The cultivation method of cutting flowers is invented by farmer Wusangong, who is earliest in Longyancun of Qingyuan county, Zhejiang province in Song Dynasty, and then the cultivation method is spread across the country. It is transmitted into Japan through monk's interaction. The cut flower cultivation of the mushroom comes from China, and the existing cut-log pure mycelium inoculation cultivation comes from Japan. By 1989, the total production of Chinese mushrooms surpassed Japan for the first time, and the Chinese mushrooms became the first major world in world production.
Piriformospora indica is a pure culturable mycorrhizal fungus that colonizes the host plant roots intracellularly and intracellularly in the form of mycelium, coils, branches or rounds. Research has shown that: the strain can promote the growth of 24 kinds of plants in 12 families, induce the plants to generate systemic resistance, improve tolerance to adversity stress, accelerate the formation of lateral roots of cuttings and promote hardening of micropropagated plants.
How to apply the microbial culture medium to the cultivation of the shiitake mushrooms is a problem of extensive research, and the existing microbial culture medium has own defects, such as long-term activity of the microbial culture medium, inexact promotion mechanism research and the like.
Disclosure of Invention
In view of the above, the invention provides a preparation method of an Indian Piriformospora straw culture medium for cultivating lentinus edodes, which optimizes the treatment process of the Indian Piriformospora so as to overcome the defects of the existing lentinus edodes culture medium and further provides a method for cultivating lentinus edodes by adopting the Indian Piriformospora straw culture medium.
The invention provides a preparation method of an Indian pyricularia indica straw culture medium for cultivating lentinus edodes, which comprises the following steps:
(1) inoculating Piriformospora indica to a Kafer culture medium, performing shake culture at the rotation speed of 120-150 r/min and the temperature of 26-30 ℃ for 6-8 days, sampling and measuring the number of viable bacteria after the culture is finished, and dissolving the culture in distilled water to ensure that the number of viable bacteria in each milliliter of solution is 1 × 106A plurality of;
(2) the number of the bacteria obtained in the step (1) is 1 × 106And (3) spraying the bacterial liquid per mL onto the surface of a straw culture medium according to the proportion of 1-15% of the inoculation amount, and fermenting at 25-37 ℃ for 12-24 h to obtain the Indian pear spore straw culture medium for cultivating the lentinus edodes.
The second aspect of the present invention provides a shiitake mushroom cultivation method, wherein the method uses the acerola straw culture medium for cultivating shiitake mushrooms obtained by the above preparation method for cultivating shiitake mushrooms, and comprises the steps of: under the aseptic condition, wiping and disinfecting the mushroom body, and inoculating mushroom flesh at the junction of a mushroom stalk and a mushroom cap into an Indian pyricularia indica straw culture medium for culturing for 50-60 days.
The invention has the beneficial effects that: the invention provides a technical basis for preparing the microbial culture medium and improving the clinical application effect of the microorganisms; according to the invention, the Piriformospora indica is added, so that the growth of the root shreds of the shiitake mushrooms can be promoted, and the yield of the shiitake mushrooms is further improved; the invention optimizes the culture of the lentinus edodes by the aid of the medium of the Piropora indica through single-factor tests, obtains optimal culture conditions, and provides technical basis for industrial mass production.
Detailed Description
The invention provides a preparation method of an Indian pyricularia indica straw culture medium for cultivating lentinus edodes, which comprises the following steps:
(1) inoculating Piriformospora indica to a Kafer culture medium, rotating at 120-150 r/min, performing shake culture at 26-30 deg.C in a rotary constant temperature speed regulation shake flask cabinet for 6-8 days, sampling to determine viable count after the culture is finished, and dissolving the culture in distilled water to make viable count per ml of solution be 1 × 106A plurality of;
(2) the number of the bacteria obtained in the step (1) is 1 × 106And (3) spraying the bacterial liquid per mL onto the surface of a straw culture medium according to the proportion of 1-15% of the inoculation amount, and fermenting at 25-37 ℃ for 12-24 h to obtain the Indian pear spore straw culture medium for cultivating the lentinus edodes.
Preferably, the formula of the straw culture medium is as follows: 62% of corn straw powder, 26% of sawdust, 10% of wheat bran, 1% of sucrose and 1% of gypsum. Crushing the raw materials according to the formula of the straw culture medium, and uniformly mixing to prepare the straw culture medium for subsequent use.
More preferably, the inoculation amount in the step (2) is 5 percent, in one embodiment of the invention, the number of the viable bacteria is 1 × 10 by fixing the fermentation strain6Inoculating each/mL of the bacterial solution into a straw culture medium, wherein the inoculation amount is 1%, 5%, 10% and 15% (V/g), and culturing at 34 ℃ for 24 h. Under aseptic condition, wiping off and sterilizing mushroom body, removing stipe, inoculating to mushroom flesh at pileus junction, and culturing at optimum culture temperature for 50-60 d. And selecting the optimal strain inoculation amount according to the growth condition of the shiitake mushrooms.
More preferably, the fermentation time in step (2) is 18 h. in one embodiment of the present invention, other culture conditions are fixed, and the viable count is 1 × 106Uniformly spraying the bacterial liquid per mL onto the surface of the straw culture medium, and culturing for 12h, 18h and 24h respectively. Under aseptic condition, wiping and sterilizing mushroom body, removing stipe, inoculating to mushroom flesh at pileus junction, and culturing at optimum culture temperature for 55 d. And selecting the optimal fermentation time according to the growth condition of the shiitake mushrooms.
Preferably, in the step (1), the Piriformospora indica is inoculated in a Kafer culture medium, and then the culture is performed for 7d at the rotation speed of 120-150 r/min and the temperature of 28 ℃ under shaking.
The second aspect of the present invention provides a shiitake mushroom cultivation method, wherein the method uses the acerola straw culture medium for cultivating shiitake mushrooms obtained by the above preparation method for cultivating shiitake mushrooms, and comprises the steps of: under the aseptic condition, wiping and disinfecting the mushroom body, and inoculating mushroom flesh at the junction of a mushroom stalk and a mushroom cap into an Indian pyricularia indica straw culture medium for culturing for 50-60 days.
Preferably, the culture time is 55 d. in one embodiment of the invention, according to the determined optimal fermentation temperature, other culture conditions are fixed, and the number of viable bacteria is 1 × 106Inoculating each/mL of the bacterial liquid into a straw culture medium, culturing for 50d, 55d and 60d respectively, and selecting reasonable culture time according to the growth condition of the shiitake mushrooms.
Preferably, the mushroom is selected from fresh mushroom fruiting bodies with normal mushroom shapes, strong growth and 7 ripeness.
The preparation method of the Piriformospora indica straw culture medium for cultivating lentinus edodes provided by the invention is further described by combining specific examples. The following examples are illustrative only and are not to be construed as limiting the invention.
The experimental procedures in the following examples are conventional unless otherwise specified. The experimental materials used in the following examples were all commercially available unless otherwise specified.
Example 1
The embodiment provides a preparation method of an Indian pyricularia indica straw culture medium for cultivating mushrooms, is used for cultivating mushrooms, and screens fermentation time in the preparation process.
The composition of the kafer medium used in this example was determined using formulations conventional in the art.
The microorganism Piriformospora indica is provided by the biomedical research institute of Yangtze university, the microorganism source is not limited to the microorganism source, and the Piriformospora indica purchased or separated in the market can obtain similar effects.
The formula of the used straw culture medium is as follows: 62% of corn straw powder, 26% of sawdust, 10% of wheat bran, 1% of sucrose and 1% of gypsum.
A preparation method of a Piriformospora indica straw culture medium for cultivating lentinus edodes comprises the following steps:
(1) inoculating Piriformospora indica to Kafer culture medium, shake culturing in rotary constant temperature speed regulating shake flask cabinet at rotation speed of 120r/min and 28 deg.C for 17 hr, sampling to determine viable count after culture, and adjusting viable count to 1 × 106one/mL.
The counting method of the viable count adopts a microscopic direct counting method. The specific operation method comprises the following steps: 1g of the culture was dissolved in 5ml of distilled water for use. Remove one piece of clean blood count board, cover a piece of coverslip in the count area. The suspension of the drug is shaken up, a small amount of the suspension is sucked up by a pipette and dropped into a counting plate. And standing for a moment and counting. The counting area is found under the low power lens, and then the high power lens is converted for observation and counting. When counting, if the counting area is composed of 16 big squares, counting the number of bacteria in the 4 big squares on the upper left, the lower left, the upper right and the lower right according to the diagonal direction. In the case of a counting area consisting of 25 large squares, the number of bacteria in the central 1 large square is counted in addition to the 4 large squares. The thallus is positioned on the double lines of the large square grids, the upper line is counted, the lower line is not counted, the left line is counted, the right line is counted, and therefore errors are reduced.
The number of bacteria per ml of suspension is equal to the average number of bacteria per cell (N) × factor (K is 4 × 10)6) × dilution factor of suspension (d).
(2) Fixing other culture conditions, and uniformly spraying the bacterial liquid obtained in the step (1) onto the surface of a straw culture medium, and respectively culturing at 28 ℃ for 12h, 18h and 24h to obtain the piriformospora indica straw culture medium for culturing mushrooms corresponding to different fermentation conditions.
The culture medium of the Piriformospora indica straw for cultivating the shiitake is respectively used for cultivating the shiitake, and the steps are as follows: selecting fresh mushroom fruiting bodies with normal, robust and 7-mature mushroom shapes as inoculation materials. Under aseptic condition, wiping and sterilizing mushroom body, removing stipe, inoculating to mushroom flesh at pileus junction, and culturing at optimum culture temperature for 55 d. After the culture is finished, the length of the root silk and the yield of the Piriformospora indica culture medium mushroom are determined to be the optimized proportion of the yield of the straw culture medium. The results are shown in Table 1.
TABLE 1 optimal ratio of length and yield of root shreds of Lentinus edodes at different fermentation times
From the results of Table 1, the optimum fermentation time was determined to be 18 hours.
Example 2
The embodiment provides a preparation method of an Indian pyricularia indica straw culture medium for cultivating mushrooms, which is used for cultivating mushrooms, and the optimal strain inoculation amount in the preparation process is screened.
(1) According to the results of example one, the fixed fermentation time was 18h, and the formulation of the medium was changed by a one-way test, and the specific formulation is shown in Table 2.
TABLE 2 formulation of Piriformospora indica straw culture medium
Crushing the raw materials according to the formula of the straw culture medium, and mixing to prepare the straw culture medium for culture and standby.
(2) The formula of the culture medium was changed by a one-factor test, and the viable count was 1 × 10 according to the test protocol of Table 26Inoculating each/mL of the bacterial liquid into a straw culture medium, wherein the inoculation amount is 1%, 5%, 10% and 15% (V/g), and culturing at 34 ℃ for 24h to obtain the Indian pear spore straw culture medium for culturing the lentinus edodes corresponding to different fermentation conditions;
the culture medium of the Piriformospora indica straw for cultivating the shiitake is respectively used for cultivating the shiitake, and the steps are as follows: selecting fresh mushroom fruiting bodies with normal, robust and 7-mature mushroom shapes as inoculation materials. Under aseptic condition, wiping and sterilizing mushroom body, removing stipe, inoculating to mushroom flesh at pileus junction, and culturing at optimum culture temperature for 50 d. The cultivation temperature of the shiitake mushrooms may be 28 ℃. The optimized ratio of the length and yield of the root filament of the medium mushroom of Piriformospora indica compared with the yield of the medium of straw is determined. The results are shown in Table 3.
TABLE 3 optimal ratio of length to unit weight of root filament of Lentinus edodes under different inoculation amounts
According to the results of table 3, the microbial culture medium was determined to be number 2, i.e., piriformospora indica: the straw culture medium is 5: 95.
Example 3
The embodiment provides a preparation method of a pyricularia indica straw culture medium for cultivating mushrooms, is used for cultivating mushrooms, and screens the optimal mushroom culture time in the preparation process.
A preparation method of a Piriformospora indica straw culture medium for cultivating lentinus edodes comprises the following steps:
(1) inoculating Piriformospora indica to Kafer culture medium, performing shake culture at 150r/min at 28 deg.C for 7d, sampling to determine viable count after the culture is finished, and dissolving the culture in distilled water to make viable count per ml of solution be 1 × 106A plurality of;
(2) the number of the bacteria obtained in the step (1) is 1 × 106And (3) spraying the bacterial liquid per mL onto the surface of a straw culture medium according to the proportion of 5% of the inoculation amount, and fermenting at 34 ℃ for 24h to obtain the Indian pear spore straw culture medium for cultivating the lentinus edodes.
The culture medium of the Piriformospora indica straw for cultivating the shiitake is respectively used for cultivating the shiitake, and the steps are as follows:
selecting fresh mushroom fruiting bodies with normal, robust and 7-mature mushroom shapes as inoculation materials. Under aseptic condition, wiping and sterilizing mushroom body, removing stipe, inoculating to mushroom flesh at pileus junction, and culturing at optimal culture temperature of Lentinus Edodes for 50d, 55d, and 60 d. The optimized ratio of the length and yield of the root filament of the medium mushroom of Piriformospora indica compared with the yield of the medium of straw is determined. The results are shown in Table 4.
TABLE 4 optimal ratio of length to weight of shiitake root shreds at each cultivation time
According to the results of Table 4, the time for culturing mushrooms was determined to be 55 d.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. A preparation method of a Piriformospora indica straw culture medium for cultivating lentinus edodes comprises the following steps:
(1) inoculating Piriformospora indica to a Kafer culture medium, performing shake culture at the rotation speed of 120-150 r/min and the temperature of 26-30 ℃ for 6-8 days, sampling and measuring the number of viable bacteria after the culture is finished, and dissolving the culture in distilled water to ensure that the number of viable bacteria in each milliliter of solution is 1 × 106A plurality of;
(2) the number of the bacteria obtained in the step (1) is 1 × 106And (3) spraying the bacterial liquid per mL onto the surface of a straw culture medium according to the proportion of 1-15% of the inoculation volume per weight, and fermenting at 25-37 ℃ for 12-24 h to obtain the Indian pear spore straw culture medium for cultivating the lentinus edodes.
2. The method for preparing the piriformospora indica straw culture medium for cultivating shiitake mushrooms according to claim 1, wherein the method comprises the following steps: the formula of the straw culture medium is as follows: 62% of corn straw powder, 26% of sawdust, 10% of wheat bran, 1% of sucrose and 1% of gypsum.
3. The method for preparing the piriformospora indica straw culture medium for cultivating shiitake mushrooms according to claim 1, wherein the method comprises the following steps: inoculating Piriformospora indica into a Kafer culture medium in the step (1), rotating at 120-150 r/min, and performing shake culture at 28 ℃ for 7 d.
4. The method for preparing the piriformospora indica straw culture medium for cultivating shiitake mushrooms according to claim 2, wherein the medium comprises: the volume/weight of the inoculation amount in the step (2) is 5 percent.
5. The method for preparing the piriformospora indica straw culture medium for cultivating shiitake mushrooms according to claim 2, wherein the medium comprises: the fermentation time in the step (2) is 18 h.
6. A shiitake mushroom cultivation method is characterized by comprising the following steps: the Piriformospora indica straw culture medium for cultivating shiitake mushrooms obtained by the preparation method of claim 1 is used for cultivating shiitake mushrooms, and comprises the following steps: under the aseptic condition, wiping and disinfecting the mushroom body, and inoculating mushroom flesh at the junction of a mushroom stalk and a mushroom cap into an Indian pyricularia indica straw culture medium for culturing for 50-60 days.
7. The shiitake mushroom cultivation method according to claim 6, characterized in that: the culture time was 55 d.
8. The shiitake mushroom cultivation method according to claim 6, characterized in that: the mushroom is selected from fresh mushroom fruiting bodies with normal mushroom shapes, strong growth and maturity of 7.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103858719A (en) * | 2014-03-11 | 2014-06-18 | 浙江大学 | Method for reducing cadmium content of overground part of paddy rice |
| WO2015001575A1 (en) * | 2013-07-05 | 2015-01-08 | Amity University | Plant growth promoting formulation of piriformospora indica and azotobacter chroococcum with talcum powder |
| CN105794455A (en) * | 2016-03-18 | 2016-07-27 | 浙江大学 | Method for utilizing piriformospora indica and Zhongshengmycin to jointly prevent and control bacterial wilt of tobaccos |
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| WO2017075023A1 (en) * | 2015-10-26 | 2017-05-04 | Ut-Battelle, Llc | Complex of mutualistic microbes designed to increase plant productivity |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015001575A1 (en) * | 2013-07-05 | 2015-01-08 | Amity University | Plant growth promoting formulation of piriformospora indica and azotobacter chroococcum with talcum powder |
| CN103858719A (en) * | 2014-03-11 | 2014-06-18 | 浙江大学 | Method for reducing cadmium content of overground part of paddy rice |
| CN105794455A (en) * | 2016-03-18 | 2016-07-27 | 浙江大学 | Method for utilizing piriformospora indica and Zhongshengmycin to jointly prevent and control bacterial wilt of tobaccos |
Non-Patent Citations (2)
| Title |
|---|
| 印度梨形孢（Piriformospora indica）的生物学特性及对植物生长的互作效应研究进展;朱志炎等;《长江大学学报（自然科学版）》;20160531;第13卷(第5期);第53-58页 * |
| 印度梨形孢培养条件优化和剂型研制及在油菜上的应用研究;刘晓曦;《中国优秀硕士学位论文全文数据库 农业科学辑》;20150815(第08期);D047-204 * |
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