CN107215860A - A kind of preparation method of the orderly self-assembled structures of graphene/DNA - Google Patents
A kind of preparation method of the orderly self-assembled structures of graphene/DNA Download PDFInfo
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- CN107215860A CN107215860A CN201610159601.6A CN201610159601A CN107215860A CN 107215860 A CN107215860 A CN 107215860A CN 201610159601 A CN201610159601 A CN 201610159601A CN 107215860 A CN107215860 A CN 107215860A
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Abstract
A kind of preparation method of the orderly self-assembled structures of graphene/DNA, this method comprises the following steps：（1）Silicon chip polyglutamic acid or the polyacrylic acid solution immersion modification of graphene will be loaded with；（2）Soaked and modified with single stranded DNA solution, i.e., in the graphenic surface formation orderly self-assembled structures of DNA.The invention provides a kind of highly versatile, the orderly self-assembled structures of graphene/DNA of functionalization, to replace the conventional self-assembled structures in rigid substrate in the prior art, application of the orderly self-assembled structures of DNA in biochemistry detection, scientific research etc. numerous areas is effectively extended.
Description
Technical field
The present invention relates to graphene composite material field, and in particular to a kind of orderly self-assembled structures of graphene/DNA and its
Preparation method.
Background technology
There is graphene the carbon atom in Colloidal particles, plane to be connected to form hexagonal lattice with sp2 hybridized orbits
Structure.Due to its unique physical property, graphene is considered as having huge in terms of sensitive electrical detection chemistry or biomolecule
Prospect.
Before the sensitive detectors based on graphene are explored, first have to find it is practicable, can be to graphene table
Face carries out the method for modifying of functionalization.Have been reported that（Mohanty, N.; Berry, V. Nano Lett. 2008, 8,
4469–4476.）The single stranded DNA that amido is ended up is by being covalently bound on graphene oxide.But what it was selected is not stone
Black alkene, but graphene oxide, and its ordered fabrication for failing to realize surface DNA.In addition, having been reported that（Graphene-DNA
hybrids: self-assembly and electrochemical detection performance）By DNA with graphite
Alkene mixes to prepare graphene aqueous solution.It utilizes interaction strong between DNA and graphene, enables the DNA sides for being dissolved in water
Graphene is helped to be dissolved in water.And ordered fabrication of the biomolecule such as DNA in graphenic surface how is realized, there is not report so far
Road.
This area need develop it is a kind of can graphenic surface ordered fabrication biomolecule method, not only make graphene
The integrality of lamella is not destroyed, can also be in graphenic surface ordered fabrication biomolecule.
The content of the invention
It is an object of the present invention to have by carrier of graphene there is provided graphene/DNA of a kind of highly versatile, functionalization
Sequence self-assembled structures, to replace the conventional self-assembled structures in rigid substrate in the prior art, effectively extend DNA in order certainly
Application of the package assembly in biochemistry detection, scientific research etc. numerous areas.
First, the invention provides a kind of preparation method of the orderly self-assembled structures of graphene/DNA, it is characterised in that should
Method comprises the following steps：
（1）Silicon chip polyglutamic acid or the polyacrylic acid solution immersion certain time of graphene will be loaded with, then spent respectively
Ionized water, absolute ethyl alcohol are cleaned, and are then dried up with nitrogen；
（2）Be dissolved with 1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides solvent be 0.01M-0.1M PBS
The single stranded DNA solution immersion of buffer solution, is then cleaned with deionized water, absolute ethyl alcohol, is then dried up with nitrogen, i.e., in stone respectively
Black alkene surface forms the orderly self-assembled structures of DNA.
Specifically, the silicon chip of graphene that is loaded with is peeled off graphene onto silicon chip using micromechanical forces stripping method.
To remove remained on surface, the silicon chip for being loaded with graphene is 1.0 × 10 in 350 DEG C of -600 DEG C of vacuums-6Pa-
1.0×10-4Vacuum annealing 2-5h is carried out under Pa, to remove the cull remained on silicon chip.
The concentration of 1- (3- the dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides used is 0.01M-0.1M.It is poly-
Glutamic acid or polyacrylic acid solution weight average molecular weight are that 20000-50000, concentration are 0.1 mg/ml-2mg/ml.
It is 0.5h-2h to take into account soak time in efficiency and stability, step (1).Step（2）Middle soak time is 6h-
24h。
Specifically, the single-stranded DNA sequence is AAAAATTTTT.The specific DNA is certainly not limited to, in the prior art institute
Be related to can in order self assembly DNA sequence dna can according to the present invention mentality of designing graphenic surface carry out self assembly.
The present invention has following technical advantage compared to prior art：
（1）The creative graphene that introduces as the substrate of DNA self assemblies, silicon different from the past, silica, mica,
Quartz etc. hard material, graphene realizes the effect of flexible substrates with its unique single layer structure；
（2）The excellent physical property of graphene effectively can be attached to the orderly self assemblies of DNA using graphene as substrate
In structure, so that the regulation and control to graphene physical property are realized, the influence of electron transport performance, its technology especially in graphene
Effect and application prospect are beyond imagination.
（3）As a kind of general preparation method, the application is widely used, the DNA sequence dna of especially existing functionalization,
It is fixed, ordering carries out scientific research, practical application to it wide significance and prospect.
Embodiment
Technical scheme is further explained below by embodiment, but protection scope of the present invention is not by reality
Apply any formal limitation of example.
Embodiment 1
1. prepare graphene on silicon chip with mechanical stripping method, the silicon chip of graphene then will be loaded with 350 DEG C, and vacuum is
1.0×10-6Vacuum annealing 2h is carried out under Pa.
2. will be loaded with the silicon chip of graphene with weight average molecular weight is polyglutamic acidleach that 20000, concentration is 0.1mg/ml
2h is steeped, is then cleaned, is then dried up with nitrogen with deionized water, absolute ethyl alcohol respectively.
3. with 1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides are dissolved with, concentration is 0.04mg/ml's
Solvent is the single stranded DNA solution immersion 8h of 0.01M PBS, is then cleaned respectively with deionized water, absolute ethyl alcohol, so
Dried up afterwards with nitrogen.DNA used sequence is AAAAATTTTT in experiment, and 1- (3- dimethylamino-propyls) -3- ethyls carbon two is sub-
The concentration of amine hydrochlorate is 0.01M.
Embodiment 2
1. prepare graphene on silicon chip with mechanical stripping method, the silicon chip of graphene then will be loaded with 450 DEG C, and vacuum is
1.0×10-4Vacuum annealing 5h is carried out under Pa.
2. will be loaded with the silicon chip of graphene with weight average molecular weight is polyglutamic acidleach that 40000, concentration is 0.5 mg/ml
1h is steeped, is then cleaned, is then dried up with nitrogen with deionized water, absolute ethyl alcohol respectively.
3. with 1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides are dissolved with, concentration is 0.08mg/ml's
Solvent is the single stranded DNA solution immersion 6h of 0.05M PBS, is then cleaned respectively with deionized water, absolute ethyl alcohol, so
Dried up afterwards with nitrogen.DNA used sequence is AAAAATTTTT in experiment, and 1- (3- dimethylamino-propyls) -3- ethyls carbon two is sub-
The concentration of amine hydrochlorate is 0.04M.
Embodiment 3
1. prepare graphene on silicon chip with mechanical stripping method, the silicon chip of graphene then will be loaded with 500 DEG C, and vacuum is
1.0×10-6Vacuum annealing 2h is carried out under Pa.
2. will be loaded with the silicon chip of graphene with weight average molecular weight is polypropylene acidleach that 30000, concentration is 1.5 mg/ml
0.8h is steeped, is then cleaned, is then dried up with nitrogen with deionized water, absolute ethyl alcohol respectively.
3. with 1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides are dissolved with, concentration is 0.06mg/ml's
Solvent is the single stranded DNA solution immersion of 0.04M PBS, is then cleaned respectively with deionized water, absolute ethyl alcohol, then
Dried up with nitrogen.DNA used sequence is AAAAATTTTT, 1- (3- dimethylamino-propyls) -3- ethyl carbodiimides in experiment
The concentration of hydrochloride is 0.05M.
Embodiment 4
1. prepare graphene on silicon chip with mechanical stripping method, the silicon chip of graphene then will be loaded with 600 DEG C, and vacuum is
1.0×10-5Vacuum annealing 3h is carried out under Pa.
2. will be loaded with the silicon chip of graphene with weight average molecular weight is polypropylene acidleach that 40000, concentration is 1.2 mg/ml
1.5h is steeped, is then cleaned, is then dried up with nitrogen with deionized water, absolute ethyl alcohol respectively.
3. with 1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides are dissolved with, concentration is 0.1mg/ml's
Solvent is the single stranded DNA solution immersion of 0.1M PBS, is then cleaned respectively with deionized water, absolute ethyl alcohol, Ran Houyong
Nitrogen is dried up.DNA used sequence is AAAAATTTTT, 1- (3- dimethylamino-propyls) -3- ethyl carbodiimide salt in experiment
The concentration of hydrochlorate is 0.1M.
Embodiment 5
1. prepare graphene on silicon chip with mechanical stripping method, the silicon chip of graphene then will be loaded with 550 DEG C, and vacuum is
1.0×10-5Vacuum annealing 4h is carried out under Pa.
2. will be loaded with the silicon chip of graphene with weight average molecular weight is polypropylene acidleach that 30000, concentration is 1.5 mg/ml
2h is steeped, is then cleaned, is then dried up with nitrogen with deionized water, absolute ethyl alcohol respectively.
3. with 1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides are dissolved with, concentration is 0.2mg/ml's
Solvent is the single stranded DNA solution immersion of 0.1M PBS, is then cleaned respectively with deionized water, absolute ethyl alcohol, Ran Houyong
Nitrogen is dried up.DNA used sequence is AAAAATTTTT, 1- (3- dimethylamino-propyls) -3- ethyl carbodiimide salt in experiment
The concentration of hydrochlorate is 0.05M.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (9)
1. a kind of preparation method of the orderly self-assembled structures of graphene/DNA, it is characterised in that this method comprises the following steps：
（1）Silicon chip polyglutamic acid or the polyacrylic acid solution immersion certain time of graphene will be loaded with, then spent respectively
Ionized water, absolute ethyl alcohol are cleaned, and are then dried up with nitrogen；
（2）Be dissolved with 1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides solvent be 0.01M-0.1M PBS
The single stranded DNA solution immersion of buffer solution, is then cleaned with deionized water, absolute ethyl alcohol, is then dried up with nitrogen, i.e., in stone respectively
Black alkene surface forms the orderly self-assembled structures of DNA.
2. the preparation method of the orderly self-assembled structures of graphene/DNA according to claim 1, it is characterised in that described negative
The silicon chip of graphene is loaded with to peel off graphene onto silicon chip using micromechanical forces stripping method.
3. the preparation method of the orderly self-assembled structures of graphene/DNA according to claim 2, it is characterised in that described negative
It is 1.0 × 10 that the silicon chip of graphene, which is loaded with, in 350 DEG C of -600 DEG C of vacuums-6Pa-1.0×10-4Vacuum annealing 2- is carried out under Pa
5h, to remove the cull remained on silicon chip.
4. the preparation method of the orderly self-assembled structures of graphene/DNA according to claim 1, it is characterised in that 1- (3-
Dimethylamino-propyl) -3- ethyl-carbodiimide hydrochlorides concentration be 0.01M-0.1M.
5. the preparation method of the orderly self-assembled structures of graphene/DNA according to claim 1, it is characterised in that described poly-
Glutamic acid or polyacrylic acid solution weight average molecular weight are that 20000-50000, concentration are 0.1 mg/ml-2mg/ml.
6. the preparation method of the orderly self-assembled structures of graphene/DNA according to claim 1, it is characterised in that step
(1) soak time is 0.5h-2h in.
7. the preparation method of the orderly self-assembled structures of graphene/DNA according to claim 1, it is characterised in that the list
Chain DNA sequence is AAAAATTTTT.
8. the preparation method of the orderly self-assembled structures of graphene/DNA according to claim 1, it is characterised in that step
（2）Middle soak time is 6h-24h.
9. the orderly self-assembled structures of graphene/DNA prepared by a kind of method limited according to any one of claim 1-8.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109534349A (en) * | 2018-03-30 | 2019-03-29 | 中国科学院上海应用物理研究所 | A kind of synthetic method and application of organic mineralized structures based on frame nucleic acid encode |
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| CN101559944A (en) * | 2009-05-27 | 2009-10-21 | 天津大学 | Conductive graphene film and self-assembly preparation method thereof |
| CN102659918A (en) * | 2012-05-16 | 2012-09-12 | 北京化工大学 | Graphene/dipeptide self-assembly composite film and preparation method thereof |
| CN102875805A (en) * | 2012-09-28 | 2013-01-16 | 浙江理工大学 | Three-dimensional graphene-poly dopamine-gold nanoparticle composite material and preparation method thereof |
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