CN107202777A

CN107202777A - A kind of small molecule chip, its construction method, its application and its detection method

Info

Publication number: CN107202777A
Application number: CN201610150174.5A
Authority: CN
Inventors: 赵�智; 张振伟; 何建安; 彭开美; 祁婷婷; 朱劲松
Original assignee: GUANGZHOU GAOTONG BIOLOGICAL TECHNOLOGY CO LTD
Current assignee: Suzhou Puxin Life Science Technology Co.,Ltd.
Priority date: 2016-03-16
Filing date: 2016-03-16
Publication date: 2017-09-26
Anticipated expiration: 2036-03-16
Also published as: CN107202777B

Abstract

The present invention relates to biochip technology field, and in particular to a kind of small molecule chip, its construction method, its application and its detection method；The small molecule chip that the present invention is provided is formed by photo-crosslinking chip and micromolecular compound chemical bonding, and wherein micromolecular compound can be with BChE albumen or BACE1 protein bindings；Present invention also offers the construction method of this small molecule chip, using this method, micromolecular compound fixed efficiency is high, good fixing effect；Present invention also offers a kind of method for detecting BChE albumen or BACE1 albumen using the small molecule chip, the method is simple to operate, and testing result is accurate.BChE albumen or BACE1 albumen in the small molecule chip detection testing sample provided using the present invention, with low cost, the stable advantage of performance.

Description

A kind of small molecule chip, its construction method, its application and its detection method

Technical field

The present invention relates to biochip technology field, more particularly to a kind of small molecule chip, its construction method, It is applied and its detection method.

Background technology

Small molecule chip is also referred to as small-molecular micro-array, is one of the important branch of biochip research.Since Since Schreiber seminars of Harvard University report small-molecular micro-array in 1999 first, small molecule chip It is widely used.The research of current small molecule chip mainly has both direction, and one is to be based on small point Basic research based on sub- chip carrier surface chemical modification and micromolecular compound immobilization strategy, two It is the application for expanding small molecule chip.In recent years, small molecule chip has been successfully applied to each hatching egg Interaction, the inhibitor screening of enzyme between screening, protein and the small molecule of white matter part, enzyme activity The research of property collection of illustrative plates, high-flux medicaments sifting, food and clinical detection and cell surface and small molecule it is mutual The fields such as relation.

Alzheimer's disease is also known as alzheimer's disease, is a kind of common disease for threatening human longevity.It More than main influence threescore the elderly cognition and capacity, the ill cycle is many decades. Current people do not understand completely yet for the Basic disease cause of Alzheimer's disease.Research discovery, butyryl courage β amyloid precusor protein lyases 1 of alkali lipase (BChE albumen) and people (BACE1 albumen) are Relevant with Alzheimer's, therefore, detection BChE albumen and BACE1 albumen are for section's lap tool There is important meaning.

In the prior art, BChE albumen and BACE1 albumen are mainly detected using Elisa methods.This side Method uses BChE albumen and BACE1 protein production antibody first, utilizes the special anti-of antigen and antibody The BChE albumen and BACE1 albumen in testing sample should be detected.The method sensitivity is high, strong, But also have certain disadvantages.For example, the antibody preparation process employed in Elisa kits is complicated, it is raw Produce cost high；And antibody mutability is inactivated, performance is unstable, and transport and carrying cost are also higher.For Disadvantage mentioned above, research and development are a kind of with low cost, performance stable detection BChE albumen or BACE1 albumen Product and method have great importance.

The content of the invention

In view of this, the invention provides a kind of small molecule chip, it can detect the BChE in testing sample Albumen or BACE1 albumen, this small molecule chip cost are cheap, and performance is stable, and testing result is accurate； In addition, present invention also offers the construction method of this small molecule chip and detection method.

In order to realize foregoing invention purpose, the present invention provides following technical scheme：

The invention provides a kind of small molecule chip, it is made up of photo-crosslinking chip and micromolecular compound, it is small Molecular compound is chemically bonded with photo-crosslinking chip；

Wherein, micromolecular compound includes 2- (1- (2,3- phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- Methyl -2- (2- amino ethoxies) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxos -4- thia -1- azabicyclos [3.2.0] heptan In alkane -2- formic acid sodium salt or 4- amino-2-hydroxybenzoic acids any one or both more than compound；

And/or；

N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl -6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) Quinazoline -4- amine, amantadine or (4- (2- chloro-4 nitrophenyls) piperidin-1-yl) (5- methyl -3- phenyl-isoxazoles Azoles -4- bases) in ketone any one or both more than compound.

In some embodiments of the invention, micromolecular compound is 2- (1- (2,3- phthalazinyl)) hydrazine carboxylic Acid ethyl ester hydrochloride salt, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridines Dioctyl phthalate methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxo -4- thia -1- nitrogen In miscellaneous bicyclic [3.2.0] heptane -2- formic acid sodium salt or 4- amino-2-hydroxybenzoic acids any one or both with On compound.

In other embodiments of the present invention, micromolecular compound is N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) Phenyl -6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, amantadine or (4- (2- Chloro-4 nitrophenyl) piperidin-1-yl) in (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone any one or both Compound above.

In other embodiments of the present invention, the micromolecular compound on small molecule chip is included 2- (1- (phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- Chlorphenyl) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetyls Amino) -7- oxo -4- thia -1- azabicyclos [3.2.0] heptane -2- formic acid sodium salts or 4- amino -2- hydroxy benzenes first In acid any one or both more than compound；And include N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl - 6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, amantadine or (4- (2- chlorine - 4- nitrobenzophenones) piperidin-1-yl) in (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone any one or both with On compound.

Preferably, in the small molecule chip that the present invention is provided, connection micromolecular compound and photo-crosslinking chip Chemical bond be C-C, C-O or C-N keys, i.e. micromolecular compound and photo-crosslinking chip pass through C-C, C-O Or the bonding of C-N keys.

Small molecule chip is unmarked small molecule chip, and any labelled reagent, work are not needed when in use Make efficiency high.

In some embodiments of the invention, photo-crosslinking chip is also chemically bonded with blank control.

Preferably, blank control is DMSO；DMSO consumption is the μ L of 1 μ L~2.

Preferably, photo-crosslinking chip is also chemically bonded with positive control.

Preferably, positive control is rapamycin.

Preferably, photo-crosslinking chip is also chemically bonded with negative control.

Preferably, negative control is BSA.

Photo-crosslinking chip is that the chip of micromolecular compound can be fixed with UV-crosslinked technology.

Preferably, photo-crosslinking chip is two-dimentional photo-crosslinking chip or three-dimensional photo-crosslinking chip.

Preferably, two-dimentional photo-crosslinking chip includes solid support, two terminal modified polyethylene glycol and light Crosslinking agent；Two terminal modified polyethylene glycol are that base group modification is determined in one end by riveting, and the other end is repaiied by conjugated group The polyethylene glycol of decorations, solid support is determined group with riveting and is connected, and photocrosslinking agent is connected with conjugated group；Riveting Determine group and be selected from-SH ,-S-S- or-SiCl₃；Conjugated group is selected from-COOH ,-OH ,-NH₂Or-OCH₃。

Preferably, three-dimensional photo-crosslinking chip include solid support, trigger composition, trigger monomer and Photocrosslinking agent；Trigger monomer by triggering composition to be connected with solid support；Photocrosslinking agent is single with triggering Body is connected, and triggers composition to include initiator and diluent, and initiator is dithiol initiator.

Present invention also offers a kind of construction method of small molecule chip, comprise the following steps：Take small molecule Compound and photo-crosslinking chip hybrid, are dried, photo-crosslinking, are cleaned, and are dried, and obtain chip, closing is not Binding site, is cleaned, and is dried, is produced.

Preferably, after taking micromolecular compound, micromolecular compound and photo-crosslinking chip hybrid step Before also comprising dissolving micromolecular compound the step of.

Preferably, the solvent of dissolving micromolecular compound is DMSO.

Preferably, when micromolecular compound is with photo-crosslinking chip hybrid, each micromolecular compound Concentration is 5mmol/L~10mmol/L.

Preferably, when micromolecular compound is with photo-crosslinking chip hybrid, each micromolecular compound Volume is the μ L of 1 μ L~2.

Preferably, micromolecular compound is with after photo-crosslinking chip hybrid, before photo-crosslinking, drying Temperature is 25 DEG C~30 DEG C.

Preferably, micromolecular compound is with after photo-crosslinking chip hybrid, before photo-crosslinking, drying Time is 1h~2h.

Preferably, photo-crosslinking is concretely comprised the following steps：With ultraviolet light 2~4 times, irradiate every time when Between be 1min~3min.

Preferably, the wavelength of ultraviolet light is 340nm~380nm, the energy of ultraviolet light is 0~9999 uJ/cm²。

It is highly preferred that the wavelength of ultraviolet light is 365nm, the energy of ultraviolet light is 0~9999uJ/cm²。

Preferably, after photo-crosslinking, dry before cleaning washing lotion for analytically pure organic solvent and Deionized water.

Preferably, in washing lotion, organic solvent be DMSO, DMF, THF, DCM, MeCN or In EtOH any one or both more than solvent.

Preferably, after photo-crosslinking, before drying, cleaning and being cleaned for ultrasonic wave.

Preferably, after photo-crosslinking, before drying, during cleaning, the number of times of every kind of washing lotion cleaning is 3 Secondary~4 times, the time cleaned every time is 5min~10min.

Preferably, the time cleaned every time is 5min.

Preferably, after cleaning, obtaining before chip, drying as nitrogen drying.

Preferably, the uncombined site of closing is specially：Coring piece and concentration are that 1mol/L, pH value are 8.5 ethanolamine solutions mixing.

In some embodiments of the invention, in the construction method for the small molecule chip that the present invention is provided, envelope The solvent for closing the ethanolamine solutions used in uncombined site is DMF.

Preferably, the temperature of mixing is 25 DEG C~30 DEG C, the time of mixing is 30min~60min.

Preferably, the time of mixing is 30min.

Preferably, after the uncombined site of closing, before drying, the washing lotion of cleaning for ethanol and go from Sub- water.

Preferably, after cleaning, obtaining before small molecule chip, drying as nitrogen drying.

Detection BChE albumen or BACE1 protein products are being prepared present invention also offers small molecule chip In application.

In small molecule chip, if micromolecular compound is 2- (1- (2,3- phthalazinyl)) hydrazine carboxylic acid's ethyl ester salt Hydrochlorate, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acid first Ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxo -4- thia -1- azabicyclos In [3.2.0] heptane -2- formic acid sodium salt or 4- amino-2-hydroxybenzoic acids any one or both more than change During compound, the small molecule chip can be used to detect BChE albumen.

In small molecule chip, if micromolecular compound is N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl - 6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, amantadine or (4- (2- chlorine - 4- nitrobenzophenones) piperidin-1-yl) in (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone any one or both with On compound when, the small molecule chip can be used to detect BACE1 albumen.

In small molecule chip, if micromolecular compound includes 2- (1- (2,3- phthalazinyl)) hydrazine carboxylic acid's ethyl ester Hydrochloride, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids Methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxo -4- thia -1- azabicyclos In [3.2.0] heptane -2- formic acid sodium salt or 4- amino-2-hydroxybenzoic acids any one or both more than change Compound, and include N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl -6- (5- ((2- mesyls ethylamino-) first Base) furans -2- bases) quinazoline -4- amine, amantadine or (4- (2- chloro-4 nitrophenyls) piperidin-1-yl) (5- methyl - 3- phenyl-isoxazole azoles -4- bases) in ketone any one or both more than compound when, the small molecule chip It can be used to detect BChE albumen and BACE1 albumen.

Present invention also offers the detection BChE albumen or BACE1 eggs of small molecule chip non-diagnostic purpose White method, comprises the following steps：

Take small molecule chip to be mixed with testing sample, be incubated, cleaning is obtained after micromolecular compound incubation Product after product and blank control are incubated, then through surface plasma resonance image-forming technology for detection, if small point Product has compared significantly (P after product is incubated with blank control after sub- compound incubation<0.05) SPR letters Number, show to contain corresponding albumen in testing sample.

Preferably, micromolecular compound be 2- (1- (2,3- phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxos -4- thia -1- azabicyclos [3.2.0] heptan In alkane -2- formic acid sodium salt or 4- amino-2-hydroxybenzoic acids any one or both the above compound when, phase The albumen answered is BChE albumen.

Preferably, micromolecular compound is N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl -6- (5- ((2- first sulphurs Acyl group ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, amantadine or (4- (2- chloro-4 nitrophenyls) Piperidin-1-yl) more than any one in (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone or both compound when, Corresponding albumen is BACE1 albumen.

Preferably, micromolecular compound comprising 2- (1- (2,3- phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxos -4- thia -1- azabicyclos [3.2.0] heptan In alkane -2- formic acid sodium salt or 4- amino-2-hydroxybenzoic acids any one or both above compound, and bag Containing N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl -6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) Quinazoline -4- amine, amantadine or (4- (2- chloro-4 nitrophenyls) piperidin-1-yl) (5- methyl -3- phenyl-isoxazoles Azoles -4- bases) more than any one in ketone or both compound when, corresponding albumen be BChE albumen and BACE1 albumen.

It was found from from embodiment 12, the inspection by small molecule chip to sample a~sample f of principal component Survey, gained testing result is consistent with actual conditions, and thus rate of accuracy reached to 100% illustrates, the present invention Small molecule chip can be used to detect BChE albumen or BACE1 albumen, testing result is accurate.

It was found from from embodiment 13, compared to existing ELISA kit, small point provided of the invention Sub- chip detection performance is stable, is easy to store and transports.In addition, small molecule chip of the present invention and ELISA Kit is compared, and is also had the advantages that with low cost.

Preferably, the time being incubated is 300s~600s, the temperature of incubation is 25 DEG C~30 DEG C.

It is furthermore preferred that the time being incubated is 300s.

Preferably, the solvent of cleaning is the mixed solution of glycine, hydrochloric acid and water, the pH of mixed solution It is worth for 2.0.It is furthermore preferred that the concentration of glycine is 10mmol/L in the solvent of cleaning.

The invention provides a kind of small molecule chip, it is made up of photo-crosslinking chip and micromolecular compound, it is small Molecular compound is chemically bonded with photo-crosslinking chip；Wherein, micromolecular compound includes 2- (1- (2,3- phenodiazines Miscellaneous naphthyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- chlorphenyls) -1,4- Dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxos It is any in -4- thia -1- azabicyclos [3.2.0] heptane -2- formic acid sodium salts or 4- amino-2-hydroxybenzoic acids Compound more than one or both；And/or；N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl -6- (5- ((2- Mesyl ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, amantadine or (4- (the chloro- 4- nitrobenzene of 2- Base) piperidin-1-yl) in (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone any one or both more than chemical combination Thing.

From embodiment 11,2- (1- (2,3- phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- Amino ethoxy) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxos -4- thia -1- azabicyclos [3.2.0] heptan Alkane -2- formic acid sodium salt or 4- amino-2-hydroxybenzoic acids can include small point of the above with BChE protein bindings The small molecule chip of sub- compound can be used to detect BChE albumen；N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) benzene Base -6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, amantadine or (4- (2- Chloro-4 nitrophenyl) piperidin-1-yl) (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone can be with BACE1 albumen knots Close, the small molecule chip for including above micromolecular compound can be used to detect BACE1 albumen.

Present invention also offers the construction method of small molecule chip, from embodiment 10, with the present invention The small molecule chip that method is built, the fixed efficiency between photo-crosslinking chip and micromolecular compound is high, Gu Determine effect good.Small molecule chip detection BChE albumen or BACE1 are utilized present invention also offers one kind The method of albumen and application, from embodiment 12, small molecule chip of the invention can be used to detect BChE Albumen or BACE1 albumen, detection method are simple, and testing result is accurate, and accuracy rate is reached for 100%. From embodiment 13, compared to ELISA kit, the small molecule chip detection property that the present invention is provided Can be stable, it is easy to store and transports.The small molecule chip that the present invention is provided use with BChE albumen or The protein bound micromolecular compounds of BACE1 detect BChE albumen or BACE1 albumen, it is to avoid The use of antibody, because antibody belongs to protein, mutability, production cost is high, micromolecular compound tool Have the advantages that property is stable, with low cost, therefore, the small molecule chip cost that the present invention is provided is cheap, Performance is stable.

Brief description of the drawings

The spr signal figure of rapamycin and FKBP12 albumen that Fig. 1 is 10mmol/L in embodiment 10.

Embodiment

The invention provides a kind of small molecule chip, its construction method, its application and its detection method.This Art personnel can use for reference present disclosure, be suitably modified technological parameter realization.In particular Be, all similar replacements and change it is apparent to those skilled in the art, they all by It is considered as and is included in the present invention.The method of the present invention and application are described by preferred embodiment, Related personnel substantially can be not departing from present invention, in spirit and scope to method described herein and should With being modified or suitably changing with combining, to realize and apply the technology of the present invention.

Used reagent and raw material can be bought by market in the embodiment that the present invention is provided.In the present invention In embodiment, raw material sources are specific as follows：

BChE albumen：Manufacturer is Sigma.

BACE1 albumen：Manufacturer is Sigma.

BChE ELISA kits：Manufacturer is EK-Bioscience.

BACE1ELISA kits：Manufacturer is EK-Bioscience.

Other raw materials and reagent are bought by market.

With reference to embodiment, the present invention is expanded on further：

The structure of the small molecule chip of embodiment 1

Take micromolecular compound 2- (1- (phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- Amino ethoxy) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxos -4- thia -1- azabicyclos [3.2.0] heptan Alkane -2- formic acid sodium salt and 4- amino-2-hydroxybenzoic acids, positive control rapamycin, by micromolecular compound It is dissolved in respectively in DMSO with positive control, concentration is 10mmol/L, pure DMSO is used as blank pair According to taking micromolecular compound and positive control, the μ L of blank control 2 respectively using high-precision automatic point sample instrument Point sample places 1h in two-dimentional photo-crosslinking chip surface under 25 DEG C (drying, room temperature)；Treat that chip is complete After drying, in ultraviolet light (wavelength 365nm, 9999uJ/cm²) under exposure 2 times, each time be 3 Min, photo-crosslinking fixes determinand；Chip is soaked in respectively the pure DMSO of analysis, DMF, THF, In DCM, MeCN, EtOH and deionized water, ultrasonic wave cleaning 5min, the respectively cleaning 3 of every kind of solvent Secondary, each scavenging period is 5min, to remove loose determinand.Fully cleaning is finished, nitrogen After drying, 1mL monoethanolamines (1M, pH=8.5) 30min is soaked at 25 DEG C, closing is uncombined Site.It is respectively washed with ethanol, deionized water, nitrogen drying covers flow cell, obtained unmarked Small molecule chip.

The structure of the small molecule chip of embodiment 2

Take micromolecular compound N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl -6- (5- ((2- mesyl ethamine Base) methyl) furans -2- bases) quinazoline -4- amine, amantadine and (4- (2- chloro-4 nitrophenyls) piperidines -1- Base) (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone, positive control rapamycin, by micromolecular compound and sun Property control be dissolved in respectively in DMSO, concentration is 5mmol/L, pure DMSO be used as blank control, profit Take micromolecular compound and positive control, the μ L point samples of blank control 2 respectively with high-precision automatic point sample instrument In three-dimensional photo-crosslinking chip surface, 1h is placed under 30 DEG C (drying room temperatures)；After chip is completely dried, In ultraviolet light (wavelength 340nm, 9999uJ/cm²) under exposure 4 times, each time be 3min, light The fixed determinand of crosslinking；Chip is soaked in analysis pure DMSO, DMF, THF and deionized water respectively In, ultrasonic wave cleaning 5min, every kind of solvent respectively cleaning 4 times, each scavenging period be 5min, with except Remove loose determinand.Fully cleaning is finished, and after nitrogen drying, 1ml second is soaked at 25 DEG C Hydramine (1M, pH=8.5) 60min, the uncombined site of closing.It is respectively washed with ethanol, deionized water, Nitrogen is dried up, and covers flow cell, obtains unmarked small molecule chip.

The structure of the small molecule chip of embodiment 3

Take micromolecular compound 2- (1- (phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- Amino ethoxy) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxos -4- thia -1- azabicyclos [3.2.0] heptan Alkane -2- formic acid sodium salt, 4- amino-2-hydroxybenzoic acids, N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl - 6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, amantadine and (4- (2- chlorine - 4- nitrobenzophenones) piperidin-1-yl) (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone, positive control rapamycin, Micromolecular compound and positive control are dissolved in DMSO respectively, concentration is 10mmol/L, pure DMSO As blank control, using high-precision automatic point sample instrument take respectively micromolecular compound and positive control, The μ L point samples of blank control 1 place 2h in two-dimentional photo-crosslinking chip surface under 25 DEG C (drying room temperatures)； After chip is completely dried, in ultraviolet light (wavelength 365nm, 0~9999uJ/cm²) under exposure 4 times, Each time is 1min, and photo-crosslinking fixes determinand；Chip is soaked in respectively analytically pure DMSO, In DMF, THF, DCM, MeCN and deionized water, ultrasonic wave cleaning 5min, every kind of solvent is each Cleaning 3 times, each scavenging period is 10min, to remove loose determinand.Fully cleaning is finished, After nitrogen drying, 1ml monoethanolamines (1M, pH=8.5) 45min is soaked at 30 DEG C, closing is not tied Close site.It is respectively washed with ethanol, deionized water, nitrogen drying covers flow cell, obtained unmarked Small molecule chip.

The structure of the small molecule chip of embodiment 4

Take micromolecular compound 2- (1- (phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, the N- (chloro- 4- (3- of 3- Fluorobenzyloxy)) phenyl -6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, sun Property control rapamycin, micromolecular compound and positive control are dissolved in DMSO respectively, concentration is 10mmol/L, pure DMSO take small point respectively as blank control using high-precision automatic point sample instrument Sub- compound and positive control, the μ L point samples of blank control 2 are in three-dimensional photo-crosslinking chip surface, and 25 DEG C (dry Dry room temperature) under place 1h；After chip is completely dried, in ultraviolet light (wavelength 365nm, 9999uJ/cm²) Lower exposure 4 times, each time is 3min, and photo-crosslinking fixes determinand；Chip is soaked in analysis respectively Pure (in DMSO, DMF, THF, DCM and deionized water, ultrasonic wave cleaning 5min, Mei Zhongrong Agent respectively cleaning 3 times, each scavenging period is 5min, to remove loose determinand.Fully cleaning Finish, after nitrogen drying, 1mL monoethanolamines (1M, pH=8.5) 30min be soaked at 25 DEG C, The uncombined site of closing.It is respectively washed with ethanol, deionized water, nitrogen drying covers flow cell, obtained Obtain unmarked small molecule chip.

The structure of the small molecule chip of embodiment 5

Take micromolecular compound 2- (1- (phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- Amino ethoxy) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, amantadine and (4- (2- chloro-4 nitrophenyls) piperidin-1-yl) (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone, positive control thunder handkerchief Mycin, micromolecular compound and positive control is dissolved in DMSO respectively, concentration is 10mmol/L, Pure DMSO takes micromolecular compound and sun respectively as blank control using high-precision automatic point sample instrument Property control, the μ L point samples of blank control 2 in two-dimentional photo-crosslinking chip surface, 25 DEG C of (drying room temperatures) decentralizations Put 1h；After chip is completely dried, in ultraviolet light (wavelength 365nm, 9999uJ/cm²) under exposure 3 times, each time is 3min, and photo-crosslinking fixes determinand；Chip is soaked in respectively analytically pure In DMSO and deionized water, ultrasonic wave cleaning 5min, every kind of solvent respectively cleaning 3 times, when cleaning every time Between be 5min, to remove loose determinand.Fully cleaning is finished, after nitrogen drying, at 25 DEG C Under be soaked in 1ml monoethanolamines (1M, pH=8.5) 30min, the uncombined site of closing.With ethanol, go Ionized water is respectively washed, nitrogen drying, is covered flow cell, is obtained unmarked small molecule chip.

The structure of the small molecule chip of embodiment 6

Take micromolecular compound (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxo -4- thias - 1- azabicyclos [3.2.0] heptane -2- formic acid sodium salt, 4- amino-2-hydroxybenzoic acids, positive control thunder handkerchief are mould Element, micromolecular compound and positive control is dissolved in DMSO respectively, concentration is 10mmol/L, Pure DMSO takes micromolecular compound and sun respectively as blank control using high-precision automatic point sample instrument Property control, the μ L point samples of blank control 2 in three-dimensional photo-crosslinking chip surface, 25 DEG C of (drying room temperatures) decentralizations Put 1h；After chip is completely dried, in ultraviolet light (wavelength 365nm, 9999uJ/cm²) under exposure 4 times, each time is 3min, and photo-crosslinking fixes determinand；Chip is soaked in the pure DMF of analysis respectively In deionized water, ultrasonic wave cleaning 5min, every kind of solvent is respectively cleaned 3 times, and each scavenging period is 5min, to remove loose determinand.Fully cleaning is finished, and after nitrogen drying, is soaked at 25 DEG C In 1ml monoethanolamines (1M, pH=8.5) 30min, the uncombined site of closing.With ethanol, deionized water It is respectively washed, nitrogen drying covers flow cell, obtains unmarked small molecule chip.

The structure of the small molecule chip of embodiment 7

Take micromolecular compound N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl -6- (5- ((2- mesyl ethamine Base) methyl) furans -2- bases) quinazoline -4- amine, positive control rapamycin, by micromolecular compound and the positive Control is dissolved in DMSO respectively, and concentration is 10mmol/L, and pure DMSO is utilized as blank control High-precision automatic point sample instrument take respectively micromolecular compound and positive control, the μ L point samples of blank control 2 in 1h is placed under two-dimentional photo-crosslinking chip surface, 25 DEG C (drying room temperature)；After chip is completely dried, In ultraviolet light (wavelength 365nm, 9999uJ/cm²) under exposure 2 times, each time be 3min, light The fixed determinand of crosslinking；Chip is soaked in analytically pure MeCN, EtOH and deionized water respectively, Ultrasonic wave cleans 5min, and every kind of solvent is respectively cleaned 3 times, and each scavenging period is 5min, to remove not Fixed determinand.Fully cleaning is finished, and after nitrogen drying, 1ml monoethanolamines are soaked at 25 DEG C (1M, pH=8.5) 30min, the uncombined site of closing.It is respectively washed with ethanol, deionized water, Nitrogen is dried up, and covers flow cell, obtains unmarked small molecule chip.

The structure of the small molecule chip of embodiment 8

Micromolecular compound 4- amino-2-hydroxybenzoic acids are taken, rapamycin is as positive control, by small point Sub- compound and positive control are dissolved in DMSO respectively, and concentration is 10mmol/L, and pure DMSO makees For blank control, BSA takes small molecule respectively as negative control using high-precision automatic point sample instrument Compound and positive control, blank control, the μ L point samples of negative control 2 are in two-dimentional photo-crosslinking chip surface, 25 DEG C 1h is placed under (drying room temperature)；After chip is completely dried, ultraviolet light (wavelength 365nm, 0~ 9999uJ/cm².) under exposure 4 times, each time be 3min, photo-crosslinking fix determinand；By chip It is soaked in respectively in analytically pure EtOH and deionized water, ultrasonic wave cleaning 5min, every kind of solvent is each clear Wash 4 times, each scavenging period is 5min, to remove loose determinand.Fully cleaning is finished, After nitrogen drying, 1ml monoethanolamines (1M, pH=8.5) 30min is soaked at 25 DEG C, closing is not Binding site.It is respectively washed with ethanol, deionized water, nitrogen drying covers flow cell, obtained without mark The small molecule chip of note.

The structure of the small molecule chip of embodiment 9

Take micromolecular compound 2- (1- (phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- Amino ethoxy) methyl -4- (2- chlorphenyls) -1,4- dihydros -3,5- pyridinedicarboxylic acids methyl ethyl ester and 4- amino -2- hydroxyls Yl benzoic acid, pure DMSO takes small molecule respectively as blank control using high-precision automatic point sample instrument Compound and the μ L point samples of blank control 2 are in two-dimentional photo-crosslinking chip surface, 25 DEG C of (drying room temperature) decentralizations Put 1h；After chip is completely dried, in ultraviolet light (wavelength 365nm, 9999uJ/cm²) under exposure 2 Secondary, each time is 3min, and photo-crosslinking fixes determinand；Chip is soaked in respectively the pure DMSO of analysis, In DMF, THF, DCM, MeCN, EtOH and deionized water, ultrasonic wave cleans 5min, every kind of Solvent respectively cleaning 3 times, each scavenging period is 5min, to remove loose determinand.It is fully clear Wash it is complete, nitrogen drying after, 1ml monoethanolamines (1M, pH=8.5) 30min is soaked at 25 DEG C, The uncombined site of closing.It is respectively washed with ethanol, deionized water, nitrogen drying covers flow cell, obtained Obtain unmarked small molecule chip.

The detection of the small molecule chip fixed efficiency of embodiment 10

The detection of small molecule chip fixed efficiency prepared by embodiment 1：

Small molecule chip prepared by Example 1, small molecule is installed according to the operation manual of SPRi instruments Chip, with PBST cushioning liquid (pH=7.4,0.05%Tween) as mobile phase, using glycine+ Hydrochloric acid (pH=2.0) aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) Live again for several times, until baseline is steady.Sample injection method is set afterwards, is passed through target proteinses FKBP12 (100nM) 300s, using glycine+hydrochloric acid (pH=2.0) aqueous solution, (concentration of glycine is afterwards 10mmol/L, addition hydrochloric acid is adjusted to pH=2.0) live again for several times, until baseline is steady.Preserve completely Data, then using DAS processed offline the data obtained, deduct after blank background, produce thunder Handkerchief mycin interacts produced spr signal, testing result as shown in figure 1, it is wrapped with target proteinses Curve (i.e. curve 1-6) obtained by 6 different monitoring points (6 repetitions), 1 control point are included (i.e. DMSO blank controls, i.e. curve 7), wherein 6 different test point curve obtaineds coincide substantially, nothing Significant difference, illustrates that the detection method stability of the present invention is good, reappearance is high；And have obvious SPR Signal value, compared with blank control spr signal, significant difference (P ＜ 0.05).As shown in Figure 1, Rapamycin has significantly (P with FKBP12 albumen<0.05) spr signal, this explanation rapamycin by It is fixed on photo-crosslinking chip, thus also knows, remaining micromolecular compound fixed with same method It has been fixed on photo-crosslinking chip, therefore, the small molecule core built using the method for the embodiment of the present invention 1 Piece, the fixed efficiency between photo-crosslinking chip and micromolecular compound is high, good fixing effect.

The detection of the fixed efficiency of small molecule chip prepared by 2~embodiment of embodiment 9：

The detection of efficiency, detection side is fixed in small molecule chip prepared by 2~embodiment of Example 9 Method is identical with the detection method of the small molecule chip of above-described embodiment 1, gained testing result and above-described embodiment The testing result of 1 small molecule chip is similar, i.e., 2~embodiment of the embodiment of the present invention 9 provides what method was built Small molecule chip, the fixed efficiency between photo-crosslinking chip and micromolecular compound is high, good fixing effect.

The small molecule chip of embodiment 11 and BACE1 albumen and BChE Protein binding assays

Sample preparation：

Sample I：Take BACE1 albumen to be mixed with physiological saline, obtain the BACE1 that concentration is 37nM Protein solution.

Sample II：Take BChE albumen to be mixed with physiological saline, the BChE eggs that concentration is 21nM are made White solution.

Sample III：Physiological saline.

Test group 1：Small molecule chip prepared by Example 3, pacifies according to the operation manual of SPRi instruments Small molecule chip is filled, with PBST cushioning liquid (pH=7.4,0.05%Tween20) as mobile phase, Using glycine+hydrochloric acid (pH=2.0) aqueous solution, (concentration of glycine is 10mmol/L, adds hydrochloric acid It is adjusted to pH=2.0) live again for several times, until baseline is steady.

After after baseline stability, passing first into after albumen FKBP12 (100nM) 300s, real-time online is seen Examine, positive control rapamycin has obvious interaction compared to blank control.

Then, the small molecule chip is lived again for several times using glycine+hydrochloric acid (pH=2.0) aqueous solution, After after baseline stability, sample (2 μ L/s, 300s), liquid of living again (3 μ L/s 300s), buffer solution (2 μ L/s, 300s), In the case where temperature is 25 DEG C, the obtained 300s of sample I is passed through, afterwards using glycine+hydrochloric acid (pH=2.0) The aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) is lived again for several times, directly It is steady to baseline.

Complete data are preserved, then using DAS processed offline the data obtained, blank are deducted After background, spr signal is observed, Kd values are calculated.

Test group 2：Compared with test group 1, it is passed through obtained sample II and replaces sample I, remaining preparation side Method and the small molecule chip of use are identical with test group 1.

Test group 3：Compared with test group 1, it is passed through obtained sample III and replaces sample I, remaining preparation side Method and the small molecule chip of use are identical with test group 1.

Obtained experimental result is as shown in the table：

The micromolecular compound of table 1 and the spr signal surveyed after being incubated after material is incubated relative to blank control

Significantly (P<0.05)

Kd values after the micromolecular compound of table 2 and institute's test substances incubation

From data above, micromolecular compound N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl -6- (5- ((2- Mesyl ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, amantadine, (4- (the chloro- 4- nitrobenzene of 2- Base) piperidin-1-yl) after (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone and sample I be incubated, it is incubated with blank control Comparing afterwards has notable (P<0.05) spr signal, Kd values respectively 24nM, 7.9nM, 11nM, Sample III and above-mentioned micromolecular compound do not have spr signal after being incubated.Because sample I is compared with sample III BACE1 albumen is added, above-mentioned micromolecular compound and sample I are incubated after being incubated relative to blank control After have notable spr signal, with sample III be incubated after there is no spr signal, it follows that small molecule chemical combination Thing N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl -6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) Quinazoline -4- amine, amantadine, (4- (2- chloro-4 nitrophenyls) piperidin-1-yl) (5- methyl -3- phenyl-isoxazoles Azoles -4- bases) ketone can be with BACE1 protein bindings.

Data above also shows, micromolecular compound 2- (1- (2,3- phthalazinyl)) hydrazine carboxylic acid's ethyl ester Salt, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids first and second Ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxo -4- thia -1- azabicyclos [3.2.0] After heptane -2- formic acid sodium salt, 4- amino-2-hydroxybenzoic acids and sample II are incubated, after being incubated with blank control Compared to there is notable (P<0.05) spr signal, Kd values be respectively 5.1nM, 0.1nM, 0.6nM, 5.0nM, sample III and above-mentioned micromolecular compound do not have spr signal after being incubated.Because sample II is relative BChE albumen is added in sample III, relative to blank pair after above-mentioned micromolecular compound and sample II incubations According to there is notable spr signal after incubation, there is no spr signal after being incubated with sample III, it follows that small point Sub- compound 2- (1- (phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- amino ethoxies) Methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl - 6- (2- phenylacetylaminos) -7- oxo -4- thia -1- azabicyclos [3.2.0] heptane -2- formic acid sodium salt, 4- amino - 2 hydroxybenzoic acid can be with BChE protein bindings.

The detection of the small molecule chip accuracy of embodiment 12

Take sample a~sample f, it is known that BChE albumen and BACE1 albumen situations in sample a~sample f It is as follows：

BChE albumen and BACE1 albumen situations in the known sample a of table 3~sample f

Albumen

Sample a

Sample b

Sample c

Sample d

Sample e

Sample f

BChE albumen

+

-

+

-

BACE1 albumen

-

+

-

+

"+" represent containing；"-" represents to be free of.

Testing sample a~testing sample f is taken, the small molecule chip that the preparation of embodiment 3 is respectively adopted is examined Survey, detection method is as follows：

Then, the small molecule chip is lived again for several times using glycine+hydrochloric acid (pH=2.0) aqueous solution, After after baseline stability, sample (2 μ L/s, 300s), liquid of living again (3 μ L/s 300s), buffer solution (2 μ L/s, 300s), In the case where temperature is 25 DEG C, above-mentioned sample a 300s are passed through, afterwards using glycine+hydrochloric acid (pH=2.0) The aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) is lived again for several times, directly It is steady to baseline.

Test group 2：Compared with test group 1, above-mentioned sample b is passed through instead of sample a, remaining preparation method Small molecule chip with use is identical with test group 1.

Test group 3：Compared with test group 1, above-mentioned sample c is passed through instead of sample a, remaining preparation method Small molecule chip with use is identical with test group 1.

Test group 4：Compared with test group 1, above-mentioned sample d is passed through instead of sample a, remaining preparation method Small molecule chip with use is identical with test group 1.

Test group 5：Compared with test group 1, above-mentioned sample e is passed through instead of sample a, remaining preparation method Small molecule chip with use is identical with test group 1.

Test group 6：Compared with test group 1, above-mentioned sample f is passed through instead of sample a, remaining preparation method Small molecule chip with use is identical with test group 1.

Obtained experimental result is as shown in the table：

The micromolecular compound of table 4 and the spr signal surveyed after being incubated after material is incubated compared to blank control

Significantly (P<0.05)

Kd values after the micromolecular compound of table 5 and institute's test substances incubation

From data above, sample b, c, f and micromolecular compound N- (the chloro- 4- of 3- (3- fluorobenzene methoxies Base)) phenyl -6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, amantadine, After (4- (2- chloro-4 nitrophenyls) piperidin-1-yl) (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone is incubated, with blank Control is compared after being incubated notable (P<0.05) spr signal, Kd values be respectively 24nM, 7.9nM, 11nM, it follows that containing the BACE1 that can be combined with above-mentioned micromolecular compound in sample b, c, f Albumen, it is consistent with the actual conditions containing the albumen in sample；Sample a, d, e and above-mentioned small molecule After compound is incubated, no spr signal shows to be free of BACE1 albumen in sample a, d, e, with sample The actual conditions for not containing the albumen are consistent.

Also known by data above, sample a, b, e and small molecule 2- (1- (2,3- phthalazinyl)) hydrazine carboxylic Acid ethyl ester hydrochloride salt, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridines Dioctyl phthalate methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxo -4- thia -1- nitrogen After miscellaneous bicyclic [3.2.0] heptane -2- formic acid sodium salt and 4- amino-2-hydroxybenzoic acids are incubated, incubated with blank control Being compared after educating has notable (P<0.05) spr signal, Kd values be respectively 5.1nM, 0.1nM, 0.6nM, 5.0nM, it follows that containing the BChE albumen that can be combined with above-mentioned small molecule in sample a, b, e, It is consistent with the actual conditions containing the albumen in sample；Sample c, d, f are incubated with above-mentioned micromolecular compound After educating, no spr signal shows to be free of BChE albumen in sample c, d, f, with not containing in sample The situation of the albumen is consistent.

Summary result of the test, the small molecule chip that the present invention is provided can be for detection BChE albumen Or BACE1 albumen, Detection accuracy is 100%.

The small molecule chip of 1~embodiment of the embodiment of the present invention 2 and 4~embodiment of embodiment 8 is used Same method detection sample a~sample f BChE albumen or BACE1 albumen, testing result and upper State testing result identical, Detection accuracy is 100%.

The Detection of Stability of the small molecule chip of embodiment 13

Test group：Small molecule chip 180 prepared by 1~embodiment of Example 9, is divided into 3 groups, 3 Group small molecular number of chips is identical with species, and every group is provided with 60 small molecule chips, wherein in fact Apply the small molecule chip 8 prepared in the small molecule chip 8 of the preparation of example 1, embodiment 2, embodiment The 3 small molecule chips 8 prepared, small molecule chip prepared by 4~embodiment of embodiment 9 is 6.

Control group：Take BChE ELISA kits and BACE1 that EK-Bioscience companies produce ELISA kit 180, the type and quantity for being divided into kit in 3 groups, 3 groups are identical, often Group sets 30 BChE ELISA kits and 30 BACE1ELISA kits.

Detected sample：Sample b, it is known that contain BChE albumen in sample b, contain BACE1 albumen.

Above-mentioned test group and control group are detected as follows：

Detection 1：The small molecule chip of 1 group of test group and the ELISA kit of 1 group of control group are taken, often Individual small molecule chip and kit carry out sample b detection on the day of production.

Detection 2：The small molecule chip of 1 group of test group and the ELISA kit of 1 group of control group are taken, often Individual small molecule chip and kit after manufacture, sample b are carried out after being placed 30 days, 30 days under normal temperature Detection.

Detection 3：The small molecule chip of 1 group of test group and the ELISA kit of 1 group of control group are taken, often Individual small molecule chip and kit after manufacture, sample b are carried out after being placed 60 days, 60 days at 60 DEG C Detection.

Statistic mixed-state result, and the accuracy rate of detection is calculated, if testing result is consistent with actual conditions, Illustrate that testing result is accurate；If testing result and actual conditions are conversely or detection fails, illustrate detection knot It is really inaccurate.

Gained testing result and accuracy rate data are calculated as follows shown in table：

The testing result of table 6 is counted

From result above, the small molecule chip of test group is in detection 1~detection 3, with small molecule The growth of chip standing time and the rise of temperature, the accuracy rate of small molecule chip detection testing sample are unchanged Change, be 100%, thus illustrate, small molecule chip detection accuracy rate be affected by the surrounding environment compared with Small, detection performance is stable.

This table is also seen that control group using ELISA kit to detect sample, in detection 1~detection 3 In, with the growth and the rise of temperature of standing time, the accuracy rate of ELISA kit detection is from 100% 30% is dropped to, is thus illustrated, the accuracy rate of ELISA kit detection is larger by surrounding environment influence, Detect that performance is unstable.

Summary experimental result is understood, compared to ELISA kit, the small molecule core of the invention provided Piece detection performance is stable, is easy to store and transports.

The small molecule chip of embodiment 14 is used for the detection of BChE albumen and BACE1 albumen

The preparation of testing sample 1：

Extraction blood 0.1mL, dilute with PBST from blood group, the atrium sinistrum of size identical adult rat 10 times are released to 1mL, BChE albumen is added thereto, the ultimate density for making BChE is 1 μ g/mL, Produce testing sample.Contain BChE albumen in the biological sample, in SIGMA-ALDRICH State, rat is from Guangzhou biological medicine and health research institute.

Small molecule chip prepared by Example 1, small molecule is installed according to the operation manual of SPRi instruments Chip, with PBST cushioning liquid (pH=7.4,0.05%Tween20) as mobile phase, uses sweet ammonia Acid+hydrochloric acid (pH=2.0) aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) Live again for several times, until baseline is steady.

Sample (2 μ L/s, 300s), liquid of living again (3 μ L/s 300s), buffer solution (2 μ L/s, 300s), in temperature Spend at 25 DEG C, to be passed through the above-mentioned 300s of testing sample 1, afterwards using glycine+hydrochloric acid (pH=2.0) The aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) is lived again for several times, directly It is steady to baseline.

Obtained experimental result is as follows：

The micromolecular compound of table 7 and the spr signal surveyed after being incubated after material is incubated relative to blank control

Significantly (P<0.05)

From data above：After micromolecular compound is incubated with testing sample 1, it is incubated with blank control Comparing afterwards has notable (P<0.05) spr signal and Kd values, show to contain BChE albumen in testing sample 1.

The small molecule chip of embodiment 15 is used for the detection of BChE albumen and BACE1 albumen

The preparation of testing sample 2：

Extraction blood 0.1mL, dilute with PBST from blood group, the atrium sinistrum of size identical adult rat 10 times are released to 1mL, BChE and BACE1 albumen is added thereto, the ultimate density for making BChE is 1 μ g/mL, BACE1 ultimate density is that 1 μ g/mL produce testing sample.Contain in the biological sample BChE albumen and BACE1 albumen, derive from SIGMA-ALDRICH China, and rat derives from Guangzhou biological medicine and health research institute.

Small molecule chip prepared by Example 6, small molecule is installed according to the operation manual of SPRi instruments Chip, with PBST cushioning liquid (pH=7.4,0.05%Tween20) as mobile phase, uses sweet ammonia Acid+hydrochloric acid (pH=2.0) aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) Live again for several times, until baseline is steady.

Sample (2 μ L/s, 300s), liquid of living again (3 μ L/s 300s), buffer solution (2 μ L/s, 300s), at 25 DEG C Under be passed through the above-mentioned 600s of testing sample 2, using glycine+hydrochloric acid (pH=2.0) aqueous solution (glycine Concentration is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) live again for several times, until baseline is steady.

Result of the test is as follows：

The micromolecular compound of table 8 and surveys material incubation after relative to blank control incubation after spr signal and Kd values

From data above：After micromolecular compound is incubated with testing sample 2, it is incubated with blank control Comparing afterwards has notable (P<0.05) spr signal and Kd values, show to contain BChE in testing sample 2 Albumen.

The small molecule chip of embodiment 16 is used for the detection of BChE albumen and BACE1 albumen

The preparation of testing sample 3：

Extraction blood 0.1mL, dilute with PBST from blood group, the atrium sinistrum of size identical adult rat 10 times are released to 1mL, BACE1 albumen is added thereto, the ultimate density for making BACE1 is 1 μ g/mL Produce testing sample.Containing BACE1 albumen but without BChE albumen, BACE1 in the biological sample Albumen source derives from Guangzhou biological medicine and health research in SIGMA-ALDRICH China, rat Institute.

Small molecule chip prepared by Example 8, small molecule is installed according to the operation manual of SPRi instruments Chip, with PBST cushioning liquid (pH=7.4,0.05%Tween20) as mobile phase, uses sweet ammonia Acid+hydrochloric acid (pH=2.0) aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) Live again for several times, until baseline is steady.

Then, the small molecule chip is lived again for several times using glycine+hydrochloric acid (pH=2.0) aqueous solution, After after baseline stability, sample (2 μ L/s, 300s), liquid of living again (3 μ L/s 300s), buffer solution (2 μ L/s, 300s), the above-mentioned 300s of testing sample 3 is passed through at 30 DEG C, glycine+hydrochloric acid (pH=2.0) water is used Solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) is lived again for several times, until Baseline is steady.

Result of the test is as follows：

The micromolecular compound of table 9 and surveys material incubation after relative to blank control incubation after spr signal and Kd values

From data above：Micromolecular compound does not have spr signal after being incubated with testing sample 3, Negative control does not show spr signal after being incubated, and shows to be free of BChE albumen in testing sample 3.

The small molecule chip of embodiment 17 is used for the detection of BChE albumen and BACE1 albumen

The preparation of testing sample 4：

Extraction blood 0.1mL, dilute with PBST from blood group, the atrium sinistrum of size identical adult rat 10 times are released to 1mL, BACE1 albumen is added thereto, the ultimate density for making BACE1 is 2ug/mL Produce testing sample.Containing BACE1 albumen but without BChE albumen, BACE1 in the biological sample Albumen source derives from Guangzhou biological medicine and health research in SIGMA-ALDRICH China, rat Institute.

Small molecule chip prepared by Example 9, small molecule is installed according to the operation manual of SPRi instruments Chip, with PBST cushioning liquid (pH=7.4,0.05%Tween20) as mobile phase, uses sweet ammonia Acid+hydrochloric acid (pH=2.0) aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) Live again for several times, until baseline is steady.

Sample (2 μ L/s, 300s), liquid of living again (3 μ L/s 300s), buffer solution (2 μ L/s, 300s), The above-mentioned 300s of testing sample 4 is passed through at 25 DEG C, it is (sweet using glycine+hydrochloric acid (pH=2.0) aqueous solution The concentration of propylhomoserin is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) live again for several times, until baseline is steady.

Result of the test is as follows：

The micromolecular compound of table 10 and surveys material incubation after relative to blank control incubation after spr signal and Kd values

From data above：Micromolecular compound does not have spr signal after being incubated with testing sample 4, Show to be free of BChE albumen in testing sample 4.

The small molecule chip of embodiment 18 is used for the detection of BChE albumen and BACE1 albumen

The preparation of testing sample 5：

Extraction blood 0.1mL, dilute with PBST from blood group, the atrium sinistrum of size identical adult rat 10 times are released to 1mL, BACE1 albumen is added thereto, the ultimate density for making BACE1 is 1 μ g/mL, Produce testing sample.Contain BACE1 albumen in the biological sample, from SIGMA-ALDRICH China, rat is from Guangzhou biological medicine and health research institute.

Small molecule chip prepared by Example 2, small molecule is installed according to the operation manual of SPRi instruments Chip, with PBST cushioning liquid (pH=7.4,0.05%Tween20) as mobile phase, uses sweet ammonia Acid+hydrochloric acid (pH=2.0) aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) Live again for several times, until baseline is steady.

After after baseline stability, passing first into after albumen FKBP12 (100nM) 300s, real-time online is seen Examine, positive control rapamycin has obvious interaction after being incubated compared to blank control.

Then, the small molecule chip is lived again for several times using glycine+hydrochloric acid (pH=2.0) aqueous solution, After after baseline stability, sample (2 μ L/s, 300s), liquid of living again (3 μ L/s 300s), buffer solution (2 μ L/s, 300s), the above-mentioned 300s of testing sample 5 is passed through at 25 DEG C, glycine+hydrochloric acid (pH=2.0) water is used Solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) is lived again for several times, until Baseline is steady.

Result of the test is as follows：

The micromolecular compound of table 11 and the SPR surveyed after being incubated after material is incubated relative to blank control Signal

From data above：After micromolecular compound is incubated with testing sample 5, it is incubated with blank control Comparing afterwards has notable (P<0.05) spr signal and Kd values, show to contain BACE1 eggs in testing sample 5 In vain.

The small molecule chip of embodiment 19 is used for the detection of BChE albumen and BACE1 albumen

The preparation of testing sample 6：

Extraction blood 0.1mL, dilute with PBST from blood group, the atrium sinistrum of size identical adult rat 10 times are released to 1mL, BChE albumen is added thereto, the ultimate density for making BChE is 2 μ g/mL, Produce testing sample.Contain BChE albumen in the biological sample, without BACE1 albumen, BChE eggs In vain from SIGMA-ALDRICH China, rat is from Guangzhou biological medicine and health research institute.

Small molecule chip prepared by Example 7, small molecule is installed according to the operation manual of SPRi instruments Chip, with PBST cushioning liquid (pH=7.4,0.05%Tween20) as mobile phase, uses sweet ammonia Acid+hydrochloric acid (pH=2.0) aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) Live again for several times, until baseline is steady.

Sample (2 μ L/s, 300s), liquid of living again (3 μ L/s 300s), buffer solution (2 μ L/s, 300s), at 25 DEG C Under be passed through the above-mentioned 300s of testing sample 6, use glycine+hydrochloric acid (pH=2.0) aqueous solution (glycine Concentration be 10mmol/L, addition hydrochloric acid is adjusted to pH=2.0) live again for several times, until baseline is steady.

Result of the test is as follows：

The micromolecular compound of table 12 and surveys material incubation after relative to blank control incubation after spr signal and Kd values

From data above：Micromolecular compound does not have spr signal after being incubated with testing sample 6, Show to be free of BACE1 albumen in testing sample 6.

The small molecule chip of embodiment 20 is used for the detection of BChE albumen and BACE1 albumen

The preparation of testing sample 3：

Extraction blood 0.1mL, dilute with PBST from blood group, the atrium sinistrum of size identical adult rat 10 times are released to 1mL, BACE1 albumen is added thereto, the ultimate density for making BACE1 is 1 μ g/mL Produce testing sample.Containing BACE1 albumen but without BChE albumen, BACE1 in the biological sample Albumen source derives from Guangzhou biological medicine and health research in SIGMA-ALDRICH China, rat Institute.Testing sample 3 in obtained testing sample 3 and embodiment 16 is identical.

Small molecule chip prepared by Example 3, small molecule is installed according to the operation manual of SPRi instruments Chip, with PBST cushioning liquid (pH=7.4,0.05%Tween20) as mobile phase, uses sweet ammonia Acid+hydrochloric acid (pH=2.0) aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) Live again for several times, until baseline is steady.

Then, the small molecule chip is lived again for several times using glycine+hydrochloric acid (pH=2.0) aqueous solution, After after baseline stability, sample (2 μ L/s, 300s), liquid of living again (3 μ L/s 300s), buffer solution (2 μ L/s, 300s), The 300s of testing sample 3 is passed through at 25 DEG C, glycine+hydrochloric acid (pH=2.0) aqueous solution (sweet ammonia is used The concentration of acid is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) live again for several times, until baseline is steady.

Obtained experimental result is as follows：

The micromolecular compound of table 13 and the spr signal surveyed after being incubated after material is incubated relative to blank control

By above results showed that micromolecular compound N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl - 6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, amantadine, (4- (the chloro- 4- of 2- Nitrobenzophenone) piperidin-1-yl) after (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone and testing sample 3 be incubated, with Blank control is compared after being incubated notable (P<0.05) spr signal and Kd values, illustrate testing sample Contain BACE1 albumen in 3.

Data above also shows, micromolecular compound 2- (1- (2,3- phthalazinyl)) hydrazine carboxylic acid's ethyl ester Salt, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids first and second Ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxo -4- thia -1- azabicyclos [3.2.0] After heptane -2- formic acid sodium salt, 4- amino-2-hydroxybenzoic acids and testing sample 3 are incubated, no spr signal, Illustrate to be free of BChE albumen in testing sample 3.

Contain BACE1 albumen in summary result of the test, testing sample 3, without BChE albumen.

The small molecule chip of embodiment 21 is used for the detection of BChE albumen and BACE1 albumen

The preparation of testing sample 7：

Blood 0.1mL is extracted from health, blood group, the atrium sinistrum of size identical adult rat, PBST is used 10 times are diluted to 1mL.Both BACE1 albumen had been free of in the biological sample or BChE albumen is free of, greatly Mouse is from Guangzhou biological medicine and health research institute.

Small molecule chip prepared by Example 4, small molecule is installed according to the operation manual of SPRi instruments Chip, with PBST cushioning liquid (pH=7.4,0.05%Tween20) as mobile phase, uses sweet ammonia Acid+hydrochloric acid (pH=2.0) aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) Live again for several times, until baseline is steady.

Sample (2 μ L/s, 300s), liquid of living again (3 μ L/s 300s), buffer solution (2 μ L/s, 300s), at 25 DEG C Under be passed through the above-mentioned 300s of testing sample 7, using glycine+hydrochloric acid (pH=2.0) aqueous solution (glycine Concentration is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) live again for several times, until baseline is steady.

Result of the test is as follows：

The micromolecular compound of table 14 and the spr signal surveyed after being incubated after material is incubated relative to blank control With Kd values

From data above：2- (1- (phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochlorides and testing sample 7 Show after incubation without spr signal in testing sample 7 without BChE albumen.

N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl -6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) Quinazoline -4- amine does not have spr signal after being incubated with testing sample 7, shows to be free of in testing sample 7 BACE1 albumen.

BACE1 albumen is free of in summary result of the test, testing sample 7, without BChE albumen.

The small molecule chip of embodiment 22 is used for the detection of BChE albumen and BACE1 albumen

The preparation of testing sample 8：

Extraction blood 0.1mL, dilute with PBST from blood group, the atrium sinistrum of size identical adult rat 10 times are released to 1mL, BChE and BACE1 albumen is added thereto, the ultimate density for making BChE is 1ug/mL, BACE1 ultimate density are that 2ug/mL produces testing sample.Contain in the biological sample BChE albumen and BACE1 albumen, derive from SIGMA-ALDRICH China, and rat derives from Guangzhou biological medicine and health research institute.

Small molecule chip prepared by Example 5, small molecule is installed according to the operation manual of SPRi instruments Chip, with PBST cushioning liquid (pH=7.4,0.05%Tween20) as mobile phase, uses sweet ammonia Acid+hydrochloric acid (pH=2.0) aqueous solution (concentration of glycine is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) Live again for several times, until baseline is steady.

Sample (2 μ L/s, 300s), liquid of living again (3 μ L/s 300s), buffer solution (2 μ L/s, 300s), at 25 DEG C Under be passed through the above-mentioned 300s of testing sample 8, using glycine+hydrochloric acid (pH=2.0) aqueous solution (glycine Concentration is 10mmol/L, and addition hydrochloric acid is adjusted to pH=2.0) live again for several times, until baseline is steady.

Result of the test is as follows：

The micromolecular compound of table 15 and the spr signal surveyed after being incubated after material is incubated relative to blank control With Kd values

From data above：2- (1- (phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- Amino ethoxy) methyl -4- (2- chlorphenyls) -1,4- dihydros -3,5- pyridinedicarboxylic acids methyl ethyl ester and testing sample 8 After incubation, there is notable (P compared with after blank control incubation<0.05) spr signal and Kd values, show to treat Contain BChE albumen in test sample product 8.

Amantadine, (4- (2- chloro-4 nitrophenyls) piperidin-1-yl) (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone After being incubated with testing sample 8, there is notable (P compared with after blank control incubation<0.05) spr signal and Kd Value, shows to contain BACE1 albumen in testing sample 8.

Contain BACE1 albumen in summary result of the test, testing sample 8, contain BChE albumen.

Described above is only the preferred embodiment of the present invention, it is noted that for the general of the art For logical technical staff, under the premise without departing from the principles of the invention, some improvement and profit can also be made Decorations, these improvements and modifications also should be regarded as protection scope of the present invention.

Claims

1. a kind of small molecule chip, it is characterised in that be made up of photo-crosslinking chip and micromolecular compound, The micromolecular compound is chemically bonded with the photo-crosslinking chip；

Wherein, the micromolecular compound comprising 2- (1- (2,3- phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- chlorphenyls) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetylaminos) -7- oxos -4- thia -1- azabicyclos [3.2.0] heptan In alkane -2- formic acid sodium salt or 4- amino-2-hydroxybenzoic acids any one or both more than compound；

And/or；

2. small molecule chip according to claim 1, it is characterised in that the photo-crosslinking chip is also It is chemically bonded with blank control.

3. small molecule chip according to claim 1 or 2, it is characterised in that the photo-crosslinking chip For two-dimentional photo-crosslinking chip or three-dimensional photo-crosslinking chip.

4. small molecule chip according to claim 3, it is characterised in that the two-dimentional photo-crosslinking core Piece includes solid support, two terminal modified polyethylene glycol and photocrosslinking agent；Described two terminal modified poly- second Glycol is that base group modification, the polyethylene glycol that the other end is modified by conjugated group, the solid are determined in one end by riveting Holder is determined group with the riveting and is connected, and the photocrosslinking agent is connected with the conjugated group；The riveting is determined Group is selected from-SH ,-S-S- or-SiCl₃；The conjugated group is selected from-COOH ,-OH ,-NH₂Or-OCH₃。

5. small molecule chip according to claim 3, it is characterised in that the three-dimensional photo-crosslinking core Piece includes solid support, triggers composition, triggers monomer and photocrosslinking agent；The initiation monomer passes through The initiation composition is connected with the solid support；The photocrosslinking agent is connected with the initiation monomer, The initiation composition includes initiator and diluent, and the initiator is dithiol initiator.

6. a kind of construction method of small molecule chip as claimed in claim 1 or 2, it is characterised in that Comprise the following steps：The micromolecular compound and the photo-crosslinking chip hybrid are taken, is dried, photo-crosslinking, Cleaning, is dried, and obtains chip, the uncombined site of closing is cleaned, dries, produce.

7. construction method according to claim 6, it is characterised in that the closing is not associated with site Specially：It is that the ethanolamine solutions that 1mol/L, pH value are 8.5 are mixed to take the chip and concentration.

8. a kind of small molecule chip as claimed in claim 1 or 2 prepare detection BuCh lipase or Application in β products of amyloid precusor protein lyases 1 of people.

9. a kind of detection BuCh lipase of small molecule chip non-diagnostic purpose as claimed in claim 2 Or the method for β amyloid precusor protein lyases 1 of people, it is characterised in that comprise the following steps：

Take the small molecule chip to be mixed with testing sample, be incubated, cleaning obtains micromolecular compound and incubated Product after rear product and blank control incubation is educated, then through surface plasma resonance image-forming technology for detection, if Product has been compared significantly after product is incubated with the blank control after the micromolecular compound is incubated (P<0.05) spr signal, shows to contain corresponding albumen in the testing sample.

10. method according to claim 9, it is characterised in that the micromolecular compound is 2- (1- (phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- Chlorphenyl) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetyls Amino) -7- oxo -4- thia -1- azabicyclos [3.2.0] heptane -2- formic acid sodium salts or 4- amino -2- hydroxy benzenes first In acid any one or both the above compound when, corresponding albumen be BuCh lipase.

11. method according to claim 9, it is characterised in that the micromolecular compound is N- (3- Chloro- 4- (3- fluorobenzyloxies)) phenyl -6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) quinazoline - 4- amine, amantadine or (4- (2- chloro-4 nitrophenyls) piperidin-1-yl) (5- methyl -3- phenyl-isoxazole azoles -4- Base) more than any one in ketone or both compound when, before corresponding β amyloids of albumen behaviour Body protein lyases 1.

12. method according to claim 9, it is characterised in that the micromolecular compound is included 2- (1- (phthalazinyl)) hydrazine carboxylic acid's carbethoxy hydrochloride, 6- methyl -2- (2- amino ethoxies) methyl -4- (2- Chlorphenyl) -1,4- dihydro -3,5- pyridinedicarboxylic acids methyl ethyl ester, (2S, 5R, 6R) -3,3- dimethyl -6- (2- phenylacetyls Amino) -7- oxo -4- thia -1- azabicyclos [3.2.0] heptane -2- formic acid sodium salts or 4- amino -2- hydroxy benzenes first In acid any one or both above compound, and include N- (the chloro- 4- of 3- (3- fluorobenzyloxies)) phenyl - 6- (5- ((2- mesyls ethylamino-) methyl) furans -2- bases) quinazoline -4- amine, amantadine or (4- (2- chlorine - 4- nitrobenzophenones) piperidin-1-yl) it is more than any one in (5- methyl -3- phenyl-isoxazole azoles -4- bases) ketone or both Compound when, corresponding albumen is that β amyloid precusor proteins of BuCh lipase and people are cracked Enzyme 1.

13. method according to claim 9, it is characterised in that the time of the incubation be 300s~ 600s, the temperature of the incubation is 25 DEG C~30 DEG C.