CN107202732A - A kind of simulation UHT processing systems heated based on micro liquid sample and its application - Google Patents
A kind of simulation UHT processing systems heated based on micro liquid sample and its application Download PDFInfo
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- CN107202732A CN107202732A CN201710399663.9A CN201710399663A CN107202732A CN 107202732 A CN107202732 A CN 107202732A CN 201710399663 A CN201710399663 A CN 201710399663A CN 107202732 A CN107202732 A CN 107202732A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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Abstract
A kind of simulation UHT processing systems heated based on micro liquid sample, the processing system is made up of carrier fluid pipe, thermal source and cooling source, the thermal source is oil bath pan, and the carrier fluid pipe body is made up of metal or alloy, and the carrier fluid pipe main body is provided at both ends with sealing device；The cooling source is ice bath pot.It is to keep high steam pressure and superelevation temperature state to be prepared with the principle for ensureing short time quick sterilization based on UHT sterilization machines that the present invention, which prepares processing system, the carrier fluid control is cheap and easy to get for material, preparation and application is simple, it is easy to operation, by adjusting oil bath temperature and oil bath time, the requirement of any heat time being related in scientific experiment operation and any heating-up temperature can be met, experiment, convenient use can be greatly simplified by being applied in the simulation sterilization process such as UHT of minor amount of experimentation sample.
Description
Technical field
It is especially a kind of to can be used for the heating of micro liquid sample the present invention relates to test-type micro liquid heater field
Simulation UHT processing systems and its application.
Background technology
Ultra high temperature short time sterilization (UHT) is a kind of to require that it is 135-140 DEG C to raise fluid temperature up to sterilizing, insulation 4-
10s process for sterilizing, whole sterilization process is moment completion at high operating temperatures, is the processing such as fresh milk, cereal beverage, fruit juice
A kind of conventional sterilization process, product can be preserved at normal temperatures after sterilizing, and the shelf-life was up to 1-6 months.Normal temperature in the market
The liquid form product of the preservation such as food such as milk, beverage class almost uses UHT process for sterilizing.Sterilized in actual production using UHT
Machine carries out the UHT processing of liquid, but the domestic most workshop in enterprise of UHT sterilization machines, and the characteristics of its is maximum is that UHT is set
It is standby to continuously run and equipment treating capacity is very big.Large-scale, pilot scale is divided into according to the difference of equipment disposal ability and lab scale UHT is set
Standby, factory is generally large-scale or pilot scale UHT sterilizers, and lab scale UHT processors are use for laboratory.Big scale production equipment treating capacity is high
Up to 5-6T/h, scale up test equipment treating capacity is 1.5-2T/h, and 10-100L/ is also wanted even if use for laboratory lab scale equipment treating capacity
H, lab scale UHT equipment is relatively small, but during practical operation must strict guarantee equipment can not dally, therefore single sample size is minimum
Also to ensure in 5-10L or so.
Different heating-up temperatures are frequently referred to product stability, nutritional ingredient, local flavor, texture in many basic research
Deng influence, and when carrying out such research, sample size is often only needed to seldom, as little as 1 liter, or even hundreds of milliliters.Such as, it is real
When testing material for fresh milk, for the milk sample pollution and influence for preventing machine milking from causing, manual milking, therefore most feelings are often required that
Milk sample quantity is few under condition.Some experiments will often consider the design and each single factor test of control group and experimental group to production
The influence of property, structure, the local flavor of product etc., therefore single processing also only has tens milliliters even several milliliters of sample size.In sample
Product amount hundreds of milliliters, tens milliliters even several milliliters it is so micro in the case of, even if the small-sized UHT equipment of use for laboratory also very
Hardly possible completes UHT processing.But need to study the composition and structure change of sample after UHT processing again to improve from the angle of basic science
UHT techniques make it be more applicable for actual production.Moreover, when studying heating-up temperature and the influence of heat time in basic science,
Generally require according to the properties of samples heat time different with the arrangements such as effect are compared or heating-up temperature, this is allowed for can in experiment
Any heating-up temperature or any heat time are used when can be related to sample treatment, and heating disinfection equipment now can not
Fully meet this requirement.In addition the miniature UHT sterilizations machine equipment of current use for laboratory is expensive, at home using equally wideless
It is general, also make it that processing sample is extremely inconvenient.
The content of the invention
For above-mentioned the deficiencies in the prior art, inventor passes through long-term technology and practical exploration, is protected with reference to UHT sterilization machines
The principle that high steam pressure and superelevation temperature state are held to ensure short time quick sterilization is based on micro liquid sample there is provided one kind
The simulation UHT processing systems of heating, it is cheap and easy to get that the processing system prepares material, and preparation and application is simple, is easy to behaviour
Make, experiment, convenient use can be greatly simplified by being applied to the UHT processing of minor amount of experimentation sample.
Specifically, the present invention relates to following technical scheme：
It is a kind of based on micro liquid sample heat simulation UHT processing systems, the processing system by carrier fluid pipe, thermal source and
Cooling source is constituted, and the thermal source is oil bath pan, and the carrier fluid pipe body is made up of metal or alloy, the carrier fluid pipe main body two ends
It is provided with sealing device；The cooling source is ice bath pot；
It is preferred that, the U-shaped pipe of carrier fluid pipe body or coil of pipes；It is easy to fix during heating, while making testing liquid exist
More uniformly spread in pipe；
It is preferred that, the pipe thickness of the carrier fluid pipe body is 0.1-1.5mm, more preferably 0.7-0.8mm；It is described
Outer tube diameter is 0.5-20mm, more preferably 5-10mm；The selection of outer tube diameter and pipe thickness is particularly important, based on most
Limits increase heat transfer area, make heat transfer more rapid consideration, outer tube diameter and pipe thickness should be as smaller as possible, but by
Need to carry out high-temperature high-voltage reaction in selected carrier fluid pipe, various liquid samples to be checked are carried in addition, metal or alloy is such a
It is more easy to occur creep effect under environment, so as to be failed because of creep-rupture；Therefore inventor is groped by constantly testing, finally
Determine above-mentioned tube wall external diameter and thickness parameter；
It is preferred that, the metal or alloy includes but is not limited to silver, copper, gold, aluminium, iron and stainless steel, more preferably
Copper, copper has the good coefficient of heat conduction, in common metal or alloy material, and its coefficient of heat conduction is extremely protruded, while copper
Chemical property is stable, and ductility is good, with good corrosion resistance；Its Portable durable, body is prepared with it to be had well
Heat-conducting effect.
It is preferred that, the sealing device is check valve, can play good self sealss effect；At present, check valve mainly makes
In waring and cooling air conditioning system, often used cooperatively with capillary；In refrigeration systems, medium flows through check valve and enters capillary
Pipe；In heating, operating mode is reverse, due to check valve unidirectional cutoff, and it can only flow into check valve heating side by side
Capillary；The present invention is based on check valve These characteristics, therefore uses the design of check valve at carrier fluid pipe body two ends, and it seals effect
It is really good；The check valve, which is provided with closure, the closure, is provided with rubber blanket for further being sealed；
It is preferred that, the carrier fluid pipe body is provided at both ends with the pressure gauge and/or thermometer being tightly connected with body, is used to
Tubular body pressure and temperature is determined, is easy to monitoring；
It is further preferred that being provided with fastener (being preferably nut) on the pressure gauge and/or thermometer, it is easy to and carries
Liquid pipe is tightly connected；
Enable to be heated evenly by the way of oil bath heating, while being easy to control temperature；Wherein, further preferably
, the oil bath pan oil bath medium is dimethicone, and the dimethicone is tasteless, nontoxic, not volatile, with high heat conduction
Property, resistant of high or low temperature, hydrophobicity and heat endurance, inventor are groped by experiment, used simultaneously using dimethyl-silicon oil medium
Above-mentioned copper carrier fluid pipe can at utmost simulate reduction ultra high temperature short time sterilization, achieve good technique effect.
It is preferred that, the cooling source is ice bath pot, is mixture of ice and water in the ice bath pot, its temperature is 0 DEG C；Through experiment
Checking, heating finished, and carrier fluid pipe, which is put into rapidly mixture of ice and water, to be cooled, it is ensured that sample in carrier fluid pipe in
Room temperature (20 DEG C) is cooled in 10s below, is on the one hand due to that mixture of ice and water temperature keeps constant using mixture of ice and water, leads to
The powerful heat exchanger effectiveness of liquid pipe is overloaded, can be quickly by micro liquid sample cooling down；Another aspect inventor passes through examination
Test from other cooling sources, such as aqueous water, brine ice, liquid nitrogen, because cooling source temperature is too high or too low, it is impossible to meet experiment
It is required that, while the heavy refrigerant such as brine ice, liquid nitrogen can damage carrier fluid tubular construction performance strongly, cause carrier fluid pipe fragility easily occur and break
It is bad, have a strong impact on carrier fluid pipe service life.
The simulation UHT processing systems based on the heating of micro liquid sample pasteurization when simulation low temperature is long
(LTLT), high temperature-short time pasteurization (HTST), superhigh temperature pasteurization and ultra high temperature short time sterilization method (UHT) sterilizing work
Application in skill.
Specifically, the application process, step is as follows：
By in testing liquid injection simulation UHT carrier fluid pipes, device is sealed, is placed in thermal source and is heated, plus
Progress cooling processing in cooling source is immediately placed in after the completion of heat treatment；
It is preferred that, it is described to heat as oil bath heating processing；The cooling source is ice bath pot, is in the ice bath pot
Mixture of ice and water；
Specifically, pasteurization (LTLT, actual sterilization conditions are 62-65 DEG C/30min) when simulation low temperature is long, uses
Oil bath is handled, and oil bath treatment temperature is 71 DEG C, and processing time is 32min；
High temperature-short time pasteurization (HTST, actual sterilization conditions are 75-90 DEG C/15-40s) is simulated, at oil bath
Reason, oil bath treatment temperature is 140 DEG C, and processing time is 30s；
Superhigh temperature pasteurize (actual 120-125 DEG C of sterilization conditions/4-15s) is simulated, is handled using oil bath, oil bath processing
Temperature is 250 DEG C, and processing time is 18s；
Ultra high temperature short time sterilization method (UHT, actual 135-140 DEG C of sterilization conditions/4-15s) is simulated, is handled using oil bath, oil
It is 270 DEG C to bathe treatment temperature, and processing time is 20s.
Beneficial effect of the present invention：
1. the present invention is by reasonable selection body material, a kind of heating tube that UHT is simulated for micro liquid sample is prepared,
The body material of body of the present invention is copper, and from the comprehensive consideration such as the coefficient of heat conduction and cost, final choice copper is used as body material.
The external diameter of main body copper pipe is suitable with material thickness, so as to increase heat transfer area to greatest extent, makes heat transfer more rapid, so as to ensure
Heat exchanger effectiveness is maximized, and realizes being rapidly heated and cooling for liquid sample, so that reduction actual production UHT as far as possible goes out
Bacterium effect；
2. the present invention makes space sealing in pipe using charging valve (check valve), heating can increase overpressure, and then make
The interior sample elevation of boiling point must be managed, the programming rate of sample also increases therewith.Experiment proves that, in pipe in space, sample can be suitable
Profit is warming up to more than its atmospheric boiling point, therefore for the fluid sample even for atmospheric boiling point less than 135 DEG C, this pipe can also
It is set to be rapidly heated to more than 135 DEG C.Such as, the boiling point of water is 100 DEG C under normal pressure, and its maximum temperature is under non-tight state
100℃；In the seal pipe of the present invention, the rise of pressure can make water be smoothly warming up to 135 DEG C and more than, therefore, the present invention
Simulation UHT heating can be carried out to it.
3. the present invention meets the requirement of micro-example treating capacity, single treatment amount is 3-50ml, and easy to operate, cost is small.
And traditional test-type UHT equipment single sample sizes are larger, complex operation, equipment is expensive, is more suitable for the actual life of enterprise
Production.The present invention is optimized from quantity of sample handling, operation and cost, and the large-scale UHT disinfection equipments for having filled up existing are unsuitable for
The blank of micro liquid sample treatment is carried out, convenient, reality is provided for the UHT sterilization processings of micro liquid sample in scientific research
The UHT processing of micro liquid sample, the practical application with highly significant are showed.
4. the present invention is by adjusting oil bath temperature and oil bath time, can meet be related in scientific experiment operation any plus
Hot time and the requirement of any heating-up temperature.Such as, different oil bath temperatures can be such that liquid in pipe is reached respectively in same time
To 65 DEG C, 85 DEG C, 105 DEG C, 125 DEG C, 135 DEG C of constant gradient temperature；Can also similarly make liquid in pipe reach it is mutually synthermal (such as
125 DEG C) the time required to form in gradient.Therefore, when the present invention can handle a series of different heating temperature and different heating
Between sample carry out scientific research, make scientific experiment design more comprehensively, analysis result is more perfect.
5. the present invention, to reach the shortening of heating-up time, makes sample reach ideal temperature as early as possible by raising oil bath temperature,
Keep suitable time in desirable temperature range, and cooled using mixture of ice and water, with ensure the sample in carrier fluid pipe in
Room temperature (20 DEG C) is cooled in 10s below.Thus closer to corresponding sterilization effect in actual production.
Brief description of the drawings
Fig. 1 is the U-shaped carrier fluid pipe schematic diagram of the application；
Wherein, 1- charging valves, 2- carrier fluid pipe bodys；
Fig. 2 (a) is U-shaped carrier fluid pipe pictorial diagram；Fig. 2 (b) is plate-like carrier fluid pipe pictorial diagram；Fig. 2 (c) is charging valve details exhibition
Diagram, wherein a-quadrant are shown as charging valve helicitic texture；
Fig. 3 is the pressure gauge being arranged on carrier fluid pipe, wherein diagram B regions are shown as fastener nut structure, and it can be with
Fig. 2 (c) a-quadrants carry out bolt connection；
Fig. 4 is the thermometer being arranged on carrier fluid pipe, wherein diagram C regions are shown as thermometer detecting head, the displaying of D regions
For for fastener nut structure, it can carry out bolt connection with Fig. 2 (c) a-quadrants；
Fig. 5 is the heating curve figure of embodiment 2；
Fig. 6 is the heating curve figure of embodiment 3；
Fig. 7 is the heating curve figure of embodiment 4；
Fig. 8 is the heating curve figure of embodiment 5；
Embodiment
It is noted that described further below is all exemplary, it is intended to provide further instruction to the application.Unless another
Indicate, all technologies used herein and scientific terminology are with usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singulative
It is also intended to include plural form, additionally, it should be understood that, when in this manual using term "comprising" and/or " bag
Include " when, it indicates existing characteristics, step, operation, device, component and/or combinations thereof.
As background technology is introduced, it there is no killed for carrying out the progress heating of micro liquid sample in the prior art
The equipment of bacterium processing；
In view of this, a kind of simulation heated based on micro liquid sample is provided in the specific embodiment of the present invention
UHT processing systems, the processing system is made up of carrier fluid pipe, thermal source and cooling source, and the thermal source is oil bath pan, the carrier fluid pipe
Body is made up of metal or alloy, and the carrier fluid pipe main body is provided at both ends with sealing device；The cooling source is ice bath pot；
In another embodiment of the invention, the U-shaped pipe of carrier fluid pipe body or coil of pipes；It is easy to fix during heating,
Testing liquid is set to be more uniformly spread in pipe simultaneously；
The pipe thickness of the carrier fluid pipe body is 0.1-1.5mm, more preferably 0.7-0.8mm；Outside the body
Footpath is 0.5-20mm, more preferably 5-10mm；The selection of outer tube diameter and pipe thickness is particularly important, based on to greatest extent
Increase heat transfer area, make heat transfer more rapid consideration, outer tube diameter and pipe thickness should be as smaller as possible, yet with selected
Carrier fluid pipe needs to carry out high-temperature high-voltage reaction, various liquid samples to be checked is carried in addition, metal or alloy is under such a environment
It is more easy to occur creep effect, so as to be failed because of creep-rupture；Therefore inventor is groped by constantly testing, final to determine
State tube wall external diameter and thickness parameter；
In another embodiment of the invention, the metal or alloy includes but is not limited to silver, copper, gold, aluminium, iron and stainless
Steel, more preferably copper, copper have the good coefficient of heat conduction, in common metal or alloy material, its coefficient of heat conduction
Extremely protrude, while copper chemical property is stable, ductility is good, with good corrosion resistance；Its Portable durable, is prepared with it
Body has good heat-conducting effect.
In another embodiment of the invention, the sealing device is check valve, can play good self sealss effect；Mesh
Before, check valve is mainly used in waring and cooling air conditioning system, is often used cooperatively with capillary；In refrigeration systems, medium is flowed through
Check valve enters capillary；In heating, operating mode is reverse, and due to check valve unidirectional cutoff, it can only flow into and list
To valve side by side heat capillary；The present invention is based on check valve These characteristics, therefore uses check valve at carrier fluid pipe body two ends
Design, its good sealing effect；The check valve, which is provided with closure, the closure, is provided with rubber blanket for sealing；
In another embodiment of the invention, the carrier fluid pipe body is provided at both ends with the pressure gauge being tightly connected with body
And/or thermometer, to determine tubular body pressure and temperature, it is easy to monitoring；
In another embodiment of the invention, it (is preferably spiral shell that fastener is provided with the pressure gauge and/or thermometer
It is female), it is easy to be connected with the carrier fluid seal of tube；
Enable to be heated evenly by the way of oil bath heating, while being easy to control temperature；Wherein, the oil bath pan oil
Bath medium is dimethicone, and the dimethicone is tasteless, nontoxic, not volatile, with high-termal conductivity, resistant of high or low temperature,
Hydrophobicity and heat endurance, inventor grope by experiment, and above-mentioned copper carrier fluid pipe is used simultaneously using dimethyl-silicon oil medium
Reduction ultra high temperature short time sterilization can be at utmost simulated, good technique effect is achieved.
In another embodiment of the invention, the cooling source is ice bath pot, is mixture of ice and water in the ice bath pot, its
Temperature is at 0 DEG C；Experiment proves that, heating is finished, and carrier fluid pipe, which is put into rapidly mixture of ice and water, to be cooled, it is ensured that carried
Sample in liquid pipe is in being cooled to room temperature (20 DEG C) below in 10s.
There is provided the simulation UHT processing systems heated based on micro liquid sample in another embodiment of the invention
Simulate low temperature it is long when pasteurization (LTLT), high temperature-short time pasteurization (HTST), superhigh temperature pasteurization and super
Application in flash pasteurization (UHT) sterilization process.
Specifically, the application process, step is as follows：
In testing liquid injection simulation UHT carrier fluid pipes, will seal device seal device and/or thermometer and/
Or pressure gauge, it is placed in thermal source and is heated, is then placed in fast cooling in cooling source；
It is preferred that, it is described to heat as oil bath heating processing；The cooling source is ice bath pot, is ice in the ice bath pot
Aqueous mixtures；
Specifically, pasteurization (LTLT, actual sterilization conditions are 62-65 DEG C/30min) when simulation low temperature is long, uses
Oil bath is handled, and oil bath treatment temperature is 71 DEG C, and processing time is 32min；
High temperature-short time pasteurization (HTST, actual sterilization conditions are 75-90 DEG C/15-40s) is simulated, at oil bath
Reason, oil bath treatment temperature is 140 DEG C, and processing time is 30s；
Superhigh temperature pasteurize (actual 120-125 DEG C of sterilization conditions/4-15s) is simulated, is handled using oil bath, oil bath processing
Temperature is 250 DEG C, and processing time is 18s；
Ultra high temperature short time sterilization method (UHT, actual 135-140 DEG C of sterilization conditions/4-15s) is simulated, is handled using oil bath, oil
It is 270 DEG C to bathe treatment temperature, and processing time is 20s.
It should be noted that the food sterilization application categories used now are various, the sterilization that different types of food is used
Technique is also not quite similar.Consider bactericidal effect, the influence to food quality and the process for sterilizing of process for sterilizing in itself
Implementation, production on frequently with low temperature it is long when pasteurization (LTLT), high temperature-short time pasteurization (HTST), superhigh temperature bar
Family name's bactericidal assay and ultra high temperature short time sterilization method (UHT) these four sterilization process.Based on this, in order that scientific research has scientific research valency
Value and application value and can further Instructing manufacture, researcher is in the different process for sterilizing of progress to food quality, structure
Influence research when, many sterilization process with reference in actual productions carry out sample heat treatments, the conventional sterilization process in production
And heating condition is shown in Table 1.
The process for sterilizing condition commonly used in the actual production of table 1
| Produce sterilization process | Heating condition |
| Pasteurization (LTLT) when low temperature is long | 62-65℃/30min |
| High temperature-short time pasteurization (HTST) | 75-90℃/15-40s |
| Superhigh temperature pasteurize | 120-125℃/4-15s |
| Ultra high temperature short time sterilization method (UHT) | 135-140℃/4-15s |
Below by instantiation, the present invention will be further elaborated, it should explanation, the description below be only for
The present invention is explained, its content is not defined.
Embodiment 1：The exploration of temperature rise curve under the conditions of different oil bath temperatures
Material and equipment：Fluid sample (fresh milk) 35ml, syringe, the simulation UHT heating carrier fluid pipe (pipes of carrier fluid pipe body
Wall thickness is 0.8mm；The outer tube diameter is 10mm, coil of pipes, maximum carrier fluid amount 50ml), thermometer, spanner, oil bath pan is (interior
Put dimethicone) and ice bath pot (built-in mixture of ice and water).
Operating method is as follows：
(1) fluid sample is slowly injected with syringe in simulation UHT heating carrier fluid pipes, heating tube one end is screwed on unidirectionally
Valve, other end, which is screwed on thermometer and screwed up with a wrench, prevents gas leakage, is placed in the oil bath pan of certain relevant temperature and heats.
(2) in heating process, survey a temperature every 5s and record, entered if oil bath temperature is improper according to actual conditions
Row adjustment, untill condition is met.
(3) according to the temperature of record, heating curve figure is drawn.
Embodiment 2：The exploration of oil bath temperature during 62-65 DEG C/30min processing
Material and operating method be the same as Example 1.Temperature is maintained at 60 DEG C of -68 DEG C of scopes during operation.According to record
Temperature, draws heating curve figure, sees Fig. 5.
In Fig. 5, oil bath temperature is 71 DEG C, and under the conditions of this oil bath, liquid in pipe sample rises to 60 DEG C of required times and is
2min, afterwards untill 30min is heated to, liquid in pipe sample temperature is no more than 68 DEG C in overall process, meets pasteurize
The heating requirements of technique.Therefore, oil bath condition is that 71 DEG C/32min is the bar that can reach 62-65 DEG C of pasteurization process/30min
Part requirement.
Embodiment 3：The exploration of oil bath temperature during 75-90 DEG C/15-40s processing
Material and operating method be the same as Example 1.Temperature is maintained at 70 DEG C of -95 DEG C of scopes during operation.According to record
Temperature, draws heating curve figure, sees Fig. 6.
In Fig. 6, oil bath temperature is 140 DEG C, and under the conditions of this oil bath, liquid in pipe sample rises to 70 DEG C of required times and is
15s, liquid in pipe sample temperature has risen to 90 DEG C when continuing to be heated to 30s.To sum up, 140 DEG C/30s is that can reach 75-90
DEG C/15-40s processing heating requirements.
Embodiment 4：The exploration of oil bath temperature during 120-125 DEG C/4-15s processing
Material and operating method be the same as Example 1.Temperature is maintained at 118 DEG C of -128 DEG C of scopes during operation.According to record
Temperature, draw heating curve figure, see Fig. 7.
In Fig. 7, oil bath temperature is 250 DEG C, and under the conditions of this oil bath, liquid in pipe sample rises to 123 DEG C of required times and is
18s, liquid in pipe sample temperature has risen to 128 DEG C when continuing to be heated to 20s.To sum up, 250 DEG C/18s is that can reach 120-
The heating requirements of 125 DEG C/4-15s processing.
Embodiment 5：The exploration of oil bath temperature during 135-140 DEG C/4-15s processing
Material and operating method be the same as Example 1.Temperature is maintained at 130 DEG C of -138 DEG C of scopes during operation.According to record
Temperature, draw heating curve figure, see Fig. 8.
In Fig. 8, oil bath temperature is 270 DEG C, and under the conditions of this oil bath, liquid in pipe sample rises to 135 DEG C of required times and is
20s.Therefore, 270 DEG C/20s oil bath condition is the heating requirements that can reach 135-140 DEG C/4-15s processing.
Embodiment 6：The exploration of the oil bath condition of four kinds of heating technique collects
The step of according to above-described embodiment 2, embodiment 3, embodiment 4 and embodiment 5, operates, and can explore and disclosure satisfy that reality
The oil bath temperature of sterilization conditions and heat time in the production of border, it sterilizes requirement and condition is shown in Table 2.
Process for sterilizing and corresponding oil bath condition in the actual production of table 2
| Process for sterilizing title | Sterilization conditions | Oil bath temperature | Oil bath condition |
| Pasteurization (LTLT) when low temperature is long | 62-65℃/30min | 71℃ | 71℃/32min |
| High temperature-short time pasteurization (HTST) | 75-90℃/15-40s | 140℃ | 140℃/30s |
| Superhigh temperature pasteurize | 120-125℃/4-15s | 250℃ | 250℃/18s |
| Ultra high temperature short time sterilization method (UHT) | 135-140℃/4-15s | 270℃ | 270℃/20s |
The time that heating in actual production is required is very short, even several seconds generally tens seconds, if heating tube heats up
Overlong time, not only results in sample loss, and the error that sample can be caused to be heat-treated is excessive, and the influence later stage divides data
Analysis is handled, therefore the heating-up time of heating liquid in pipe is considered during heating operation.So, by raise oil bath temperature with up to
To the shortening of heating-up time, sample is reached ideal temperature as early as possible, suitable time is kept in desirable temperature range, and use ice
Aqueous mixtures ensure that sample in carrier fluid pipe in being cooled to room temperature (20 DEG C) in 10s below.Thus closer to actual production
In corresponding sterilization effect.Therefore, embodiment 1-5 explores a series of temperature rise curve under the conditions of oil bath temperatures to instruct reality
Operation.Embodiment result shows that heating tube programming rate of the invention is fast, and the time is short, and required temperature is can reach in general 15 seconds
Degree, it is actual to carry out that its heating-up time can be neglected during heating operation, it is fully able to meet the superhigh temperature pasteurize in actual production
With UHT processing.The heating tube single treatment amount of the present invention is 3-50ml, meets what micro liquid sample was heat-treated to low-voltage high-temperature
It is required that, the large-scale UHT disinfection equipments for having filled up existing are unsuitable for the blank of micro liquid sample treatment, are micro in scientific research
It is convenient that the UHT sterilization processings of fluid sample are provided, and realizes the UHT processing of micro liquid sample, the reality with highly significant
Border application.
Embodiment 7：The measure of pressure under the conditions of four kinds of process for sterilizing
Material and equipment：Fluid sample (fresh milk), syringe, simulation UHT heating carrier fluid pipes, pressure gauge, spanner, oil bath pan
(built-in dimethicone) and ice bath pot (built-in mixture of ice and water).
Operating method is as follows：
(1) fluid sample is slowly injected with syringe in simulation UHT heating carrier fluid pipes, heating tube one is screwed on double-deck close
Capping, other end, which is screwed on pressure gauge and screwed up with a wrench, prevents gas leakage, is placed in the oil bath pan of certain relevant temperature and heats.
(2) in heating process, survey a pressure every 5s and record.
(3) pressure value when different temperatures under summary record, different time.Such as table 3.
Overpressure under the conditions of 3 four kinds of process for sterilizing of table
The preferred embodiment of the application is the foregoing is only, the application is not limited to, for the skill of this area
For art personnel, the application can have various modifications and variations.It is all within spirit herein and principle, made any repair
Change, equivalent substitution, improvement etc., should be included within the protection domain of the application.
Claims (10)
1. a kind of simulation UHT processing systems heated based on micro liquid sample, it is characterised in that the processing system is by carrier fluid
Pipe, thermal source and cooling source composition, the thermal source is oil bath pan, and the carrier fluid pipe body is made up of metal or alloy, the carrier fluid
Pipe main body is provided at both ends with sealing device, and the cooling source is ice bath pot.
2. processing system as claimed in claim 1, it is characterised in that the U-shaped pipe of carrier fluid pipe body or coil of pipes.
3. processing system as claimed in claim 2, it is characterised in that the pipe thickness of the carrier fluid pipe body is 0.1-1.5mm,
More preferably 0.7-0.8mm；The outer tube diameter is 0.5-20mm, more preferably 5-10mm.
4. processing system as claimed in claim 1, it is characterised in that the metal or alloy include but is not limited to silver, copper, gold,
Aluminium, iron and stainless steel, preferably copper.
5. processing system as claimed in claim 1, it is characterised in that the sealing device is check valve；The check valve is set
Have in closure, the closure and be provided with rubber blanket.
6. processing system as claimed in claim 1, it is characterised in that the carrier fluid pipe body, which is provided at both ends with to seal with body, to be connected
The pressure gauge and/or thermometer connect；
Fastener (being preferably nut) is provided with the pressure gauge and/or thermometer, is easy to be connected with the carrier fluid seal of tube.
7. processing system as claimed in claim 1, it is characterised in that the oil bath pan oil bath medium includes but is not limited to dimethyl
The conventional oil bath medium such as silicone oil, preferably dimethicone；
The cooling source is ice bath pot, is mixture of ice and water in the ice bath pot, its temperature is 0 DEG C.
8. the simulation UHT processing systems based on the heating of micro liquid sample described in claim any one of 1-7 are long in simulation low temperature
When pasteurization (LTLT), high temperature-short time pasteurization (HTST), superhigh temperature pasteurization and ultra high temperature short time sterilization
Application in method (UHT) sterilization process.
9. application as claimed in claim 8, it is characterised in that the application process, step is as follows：
By in testing liquid injection simulation UHT carrier fluid pipes, device and/or thermometer and/or pressure gauge are sealed, thermal source is placed in
It is middle to be heated, progress cooling processing in cooling source is immediately placed in after the completion of heating；
Described heat is oil bath heating, and the cooling source is ice bath pot, is mixture of ice and water in the ice bath pot.
10. applied as described in claim 8 or 9, it is characterised in that pasteurization (LTLT, actual sterilization when simulation low temperature is long
Condition is 62-65 DEG C/30min), handled using oil bath, oil bath treatment temperature is 71 DEG C, and processing time is 32min；
High temperature-short time pasteurization (HTST, actual sterilization conditions are 75-90 DEG C/15-40s) is simulated, is handled using oil bath, oil
It is 140 DEG C to bathe treatment temperature, and processing time is 30s；
Superhigh temperature pasteurize (actual 120-125 DEG C of sterilization conditions/4-15s) is simulated, is handled using oil bath, oil bath treatment temperature
For 250 DEG C, processing time is 18s；
Ultra high temperature short time sterilization method (UHT, actual 135-140 DEG C of sterilization conditions/4-15s) is simulated, is handled using oil bath, at oil bath
It is 270 DEG C to manage temperature, and processing time is 20s.
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Application publication date: 20170926 |