CN107199230A - A kind of method of the mould fermentation process bent wine grain of shield shell - Google Patents

A kind of method of the mould fermentation process bent wine grain of shield shell Download PDF

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Publication number
CN107199230A
CN107199230A CN201710289566.4A CN201710289566A CN107199230A CN 107199230 A CN107199230 A CN 107199230A CN 201710289566 A CN201710289566 A CN 201710289566A CN 107199230 A CN107199230 A CN 107199230A
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Prior art keywords
bent wine
wine grain
shield shell
bent
fermentation process
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CN201710289566.4A
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Chinese (zh)
Inventor
刘勇
黄小琴
伍文宪
张蕾
尹全
刘红雨
薛龙海
周西全
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Institute of Plant Protection Sichuan Academy of Agricultural Sciences
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Institute of Plant Protection Sichuan Academy of Agricultural Sciences
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Priority to CN201710289566.4A priority Critical patent/CN107199230A/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09BDISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
    • B09B3/00Destroying solid waste or transforming solid waste into something useful or harmless
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09BDISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
    • B09B5/00Operations not covered by a single other subclass or by a single other group in this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/145Fungal isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
  • Mycology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Environmental & Geological Engineering (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The method that the present invention provides a kind of mould fermentation process bent wine grain of shield shell, comprises the following steps:1) bacterial strain is activated:Shield shell trichoderma strain is inoculated in PDA culture medium, cultivated 10 14 days in 18 23 DEG C, obtains activating conidium;2) preparation of spore suspension:Conidial suspension concentration is adjusted to 105‑107Individual spore/mL;3) bent wine grain processing:Bent wine grain is subjected to microwave treatment, then in the 20min that sterilized under HTHP;4) fermentation process:By step 2) spore suspension add in the bent wine grain of sterilized processing, the bent wine grain and the solid-to-liquid ratio m of spore suspension:V=6 8:1, in fermentation process 14 18 days at 18 23 DEG C.The poor processing method of bent wine that the present invention is provided, possesses that processing procedure is simple and convenient, processing time is short, can preferably realize the harmless treatment of bent wine grain, improve crude protein content in bent wine grain, and can decompose the rice husk in bent wine grain very well.

Description

A kind of method of the mould fermentation process bent wine grain of shield shell
Technical field
The invention belongs to field of agricultural microbial technology, and in particular to a kind of method of the mould fermentation process bent wine grain of shield shell.
Background technology
China is drinks production and consumption big country, and substantial amounts of solid waste can be produced in the production process of production drinks Thing, i.e. vinasse, it was reported that the vinasse that China produces every year have exceeded 10,000,000 tons, and in the generation of first half of the year vinasse in 2013 Amount has just reached more than 20,000,000 tons.Because fresh vinasse moisture is high, acidity is high, if handling easily corruption change not in time Can matter, can both cause the wasting of resources, and wine brewing ecological environment is polluted, therefore carry out harmless treatment and money to vinasse Can the abundant trans-utilization in source, basis for the survival for a long time as liquor-making enterprise.
Bent wine grain is the main discarded object produced by white spirit brewing enterprise, is that the raw grains such as sorghum, wheat, cereal pass through distillation Extract the residue after alcohol.Bent wine grain is made up of organic matters such as water, rice husk, starch, sugar, protein, organic acids, is initially contained Water rate is about 60%, additionally containing inorganic constituents such as a small amount of calcium, phosphorus, potassium and silicon.Starch in bent wine grain, protein, have The materials such as machine acid, carbohydrate are the nutritional ingredient needed for biological growth in itself, and are belonged in rice husk containing lignin, cellulose etc. Flammable organic matter, calcium, phosphorus, potassium etc. are the important composition compositions of inorganic fertilizer, and inorganic silicon is then the main component of waterglass.
The existing processing method to vinasse mainly has following several:(1) using energy source of vinasse, including directly burning, work Alcohol fuel, gasification production combustion gas are produced for fermenting raw materials production biogas energy, as raw material;(2) vinasse are used to produce feed, Including vinasse convection drying production feed, ensiling production ensilage, fermentation production of protein feedstuff;(3) industrial chemicals, bag are produced Include production xylitol, production succinic acid, production glycerine, production lactic acid etc.;(4) Applied Materials, including production cellulose, life are produced Produce activated carbon etc.;(6) production organic fertilizer etc..
In the above-mentioned processing method for vinasse, vinasse are used to produce forage protein, possess that technology is simple and easy to apply, scale can Greatly can be small, but its economic benefit is not high, crude protein content is not high in feed, and product market is restricted, and the rice in vinasse Influence of the shell to feeding quality is larger;Vinasse anaerobic fermentation produces biogas, possesses that fermenting-ripening degree is good, operating cost is low, still Floor space is larger, and rice husk is unfermentable, it is impossible to individually handled using the technology;By the way of directly burning, place Reason is thorough and can be as using energy source, but burning easily brings secondary pollution problem.Above-mentioned processing mode, in the presence of certain Limitation, especially for containing a large amount of rice husks bent wine grain, because the rice husk in vinasse can not be effectively treated, to wine The fermentation process of grain brings certain difficulty.Accordingly, it is desirable to provide a kind of method of the processing vinasse of simple and effective, for wine brewing Enterprise solves the problem of rice husk is handled in vinasse.
The content of the invention
The purpose of the present invention provides a kind of side of the mould fermentation process bent wine grain of shield shell exactly in order to solve the above problems Method, possesses that processing procedure is simple and convenient, processing time is short, can realize the harmless treatment of bent wine grain, improve thick in bent wine grain Protein content, and can effectively decompose the rice husk in bent wine grain.
To achieve these goals, the technical solution adopted by the present invention is:
A kind of method of the mould fermentation process bent wine grain of shield shell, comprises the following steps:
1) bacterial strain is activated:Shield shell trichoderma strain is inoculated in PDA culture medium, cultivates 10-14 days, is lived in 18~23 DEG C Change conidium;
2) preparation of spore suspension:With aseptic water washing step 1) gained activation conidium, adjust conidium and hang Supernatant liquid concentration is to 105~107Individual spore/mL;
3) bent wine grain processing:Bent wine grain is subjected to microwave treatment, then in the 20min that sterilized under HTHP;
4) fermentation process:By step 2) gained spore suspension add through step 3) processing after bent wine grain in, the song The solid-to-liquid ratio m of vinasse and spore suspension:V=6~8:1, it is 18~23 DEG C, fermentation process 14-18 days to control temperature.
Applicants have unexpectedly found that, the method provided according to the present invention carries out shield shell mould add at fermentation in bent wine grain Reason, can realize the effective, harmlessization processing of bent wine grain, shorter to the processing time of bent wine grain, improve the thick egg in bent wine grain Bai Hanliang, and to the high treating effect of rice husk in bent wine grain, the resolution ratio of rice husk is high.
Be used as preferred, of the invention step 1) described in shield shell it is mould be that (deposit number is the mould Cm2004 of shield shell:CGMCC NO.3852), using the mould processing bent wine grain of the shield shell, the characteristics of rice husk resolution ratio is higher with treatment effect more preferably.
Be used as preferred, step 1) described in the pH of PDA culture medium be 5.5-6.5, it is preferably under the conditions of the medium pH The mould Cm2004 of shield shell grown cultures, can obtain the activation spore of higher concentration.
Be used as preferred, step 2) in the adjustment spore suspension to 106To song under individual spore/mL, the spore concentration The high treating effect of vinasse, the resolution ratio of rice husk is higher.
Be used as preferred, step 3) described in the operation of microwave treatment be:The water content for adjusting bent wine grain is 30%, then In handling 15min under 500W microwave power.
Be used as preferred, step 3) described in the condition of HTHP be:Steam pressure 130kPa, 125 DEG C of temperature.
Be used as preferred, step 4) in, to pass through step 3) processing bent wine grain in add carbon source, then by spore suspension Liquid is added in bent wine grain, and the mould fermentation of shield shell can be more conducive to by adding carbon source.
As preferred, the carbon source is one kind in glucose, sucrose, maltose.
As preferred, the addition of the carbon source, according to mass percent meter, the 1~1.5% of the poor quality of bent wine is accounted for.
Beneficial effects of the present invention are as follows:
1st, the harmless treatment of bent wine grain can be achieved, treatment effeciency is high, and processing time is shorter;
2nd, crude protein content in bent wine grain can be improved, enhancing rate is up to more than 65%;Can preferably it decompose in bent wine grain Rice husk, rice husk resolution ratio is close to 50%.
Embodiment
In order that technical scheme and effect are clearer, the present invention is carried out specifically with reference to embodiments It is bright.It should be pointed out that following examples are used only for explaining the concrete scheme of the present invention, it is not intended to limit the present invention.
Embodiment 1
Processing bent wine is poor in accordance with the following methods:
1) bacterial strain is activated:Shield shell trichoderma strain is inoculated in the PDA culture medium that pH is 5.5, cultivates 14 days, obtain in 18 DEG C Activate conidium;
2) preparation of spore suspension:With aseptic water washing step 1) gained activation conidium, adjust conidium and hang Supernatant liquid concentration is to 105Individual spore/mL;
3) bent wine grain processing:The water content for adjusting bent wine grain is 30%, then in carrying out microwave under 500W microwave power 15min is handled, then in (steam pressure 130kPa, 125 DEG C of temperature) sterilizing 20min under HTHP;
4) fermentation process:By step 2) gained spore suspension add through step 3) processing after bent wine grain in, the song The solid-to-liquid ratio m of vinasse and spore suspension:V=6:1, it is 18 DEG C, fermentation process 18 days to control temperature.
The mould shield shell is the mould Cm2004 of shield shell, and deposit number is:CGMCC NO.3852.
Embodiment 2
Processing bent wine is poor in accordance with the following methods:
1) bacterial strain is activated:Shield shell trichoderma strain is inoculated in the PDA culture medium that pH is 6.0, cultivates 12 days, obtain in 20 DEG C Activate conidium;
2) preparation of spore suspension:With aseptic water washing step 1) gained activation conidium, adjust conidium and hang Supernatant liquid concentration is to 106Individual spore/mL;
3) bent wine grain processing:The water content for adjusting bent wine grain is 30%, then in carrying out microwave treatment under 500W power 15min, then in (steam pressure 130kPa, 125 DEG C of temperature) sterilizing 20min under HTHP;
4) fermentation process:By step 2) gained spore suspension add through step 3) processing after vinasse in, the bent wine Grain and the solid-to-liquid ratio m of spore suspension:V=7:1, it is 20 DEG C, fermentation process 16 days to control temperature.
The mould shield shell is the mould Cm2004 of shield shell, and deposit number is:CGMCC NO.3852.
Embodiment 3
Processing bent wine is poor in accordance with the following methods:
1) bacterial strain is activated:Shield shell trichoderma strain is inoculated in the PDA culture medium that pH is 6.5, cultivates 10 days, obtain in 23 DEG C Activate conidium;
2) preparation of spore suspension:With aseptic water washing step 1) gained activation conidium, adjust conidium and hang Supernatant liquid is to 107Individual spore/mL;
3) bent wine grain processing:The water content for adjusting bent wine grain is 30%, then in carrying out microwave treatment under 500W power 15min, then in (steam pressure 130kPa, 125 DEG C of temperature) sterilizing 20min under HTHP;
4) fermentation process:By step 2) gained spore suspension add through step 3) processing after bent wine grain in, the song The solid-to-liquid ratio m of vinasse and spore suspension:V=8:1, it is 23 DEG C, fermentation process 14 days to control temperature.
The mould shield shell is the mould Cm2004 of shield shell, and deposit number is:CGMCC NO.3852.
Embodiment 4
According to the method processing bent wine grain of embodiment 3, wherein to through step 3) glucose is added in treated bent wine grain, Addition is the 1% of the poor quality of bent wine, then adds the spore suspension into bent wine grain again.
Embodiment 5
According to the method processing bent wine grain of embodiment 3, wherein to through step 3) sucrose is added in treated bent wine grain, plus It is the 1.5% of the poor quality of bent wine to enter amount, then adds the spore suspension into bent wine grain again.
Test example 1
Bent wine grain after to being handled in embodiment 1-5 is detected, and is contrasted with the bent wine grain of before processing, and detection is bent The content and water-content indicator of crude protein content, rice husk in vinasse, acquired results are as shown in table 1:
Table 1
As it can be seen from table 1 the poor processing method of the bent wine of the present invention, it is possible to increase crude protein content in bent wine grain, lifting Rate is higher to the rice husk resolution ratio in bent wine grain up to more than 65%, and up to 50%.

Claims (9)

1. a kind of method of the mould fermentation process bent wine grain of shield shell, it is characterised in that methods described comprises the following steps:
1) bacterial strain is activated:Shield shell trichoderma strain is inoculated in PDA culture medium, cultivated 10-14 days in 18~23 DEG C, activation point is obtained Raw spore;
2) preparation of spore suspension:With aseptic water washing step 1) gained activation conidium, adjust conidial suspension Concentration is to 105~107Individual spore/mL;
3) bent wine grain processing:Bent wine grain is subjected to microwave treatment, then in the 20min that sterilized under HTHP;
4) fermentation process:By step 2) gained spore suspension added through step 3) in bent wine grain after processing, bent wine grain With the solid-to-liquid ratio m of spore suspension:V=6~8:1, it is 18~23 DEG C, fermentation process 14-18 days to control temperature.
2. mould shield shell described according to the method described in claim 1, it is characterised in that step 1) is the mould Cm2004 of shield shell, protect Hiding numbering is:CGMCC NO.3852.
3. the pH of PDA culture medium described according to the method described in claim 1, it is characterised in that step 1) is 5.5-6.5.
4. the spore suspension is adjusted according to the method described in claim 1, it is characterised in that step 2) to 106Individual spore Son/mL.
5. the operation of microwave treatment described according to the method described in claim 1, it is characterised in that step 3) is:Adjustment is bent The water content of vinasse is 30%, then in handling 15min under 500W microwave power.
6. the condition of HTHP described according to the method described in claim 1, it is characterised in that step 3) is:Vapour pressure Power 130kPa, 125 DEG C of temperature.
7. according to the method described in claim 1, it is characterised in that step 4), to passing through step 3) in the bent wine grain of processing Carbon source is added, then adds spore suspension in bent wine grain.
8. method according to claim 7, it is characterised in that the carbon source is one in glucose, sucrose and maltose Kind.
9. method according to claim 7, it is characterised in that the addition of the carbon source, according to mass percent meter, is accounted for The 1~1.5% of bent wine grain quality.
CN201710289566.4A 2017-04-27 2017-04-27 A kind of method of the mould fermentation process bent wine grain of shield shell Pending CN107199230A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104256057A (en) * 2014-10-01 2015-01-07 青岛嘉瑞生物技术有限公司 Method for preparing feed proteins by utilizing alcohol waste liquor and crop straws
CN104273378A (en) * 2014-10-10 2015-01-14 青岛嘉瑞生物技术有限公司 New process for producing fermented feed for dairy cows by taking wheat straw and distillers' grains as raw materials
CN104759445A (en) * 2015-03-12 2015-07-08 四川省农业科学院植物保护研究所 Harmless treatment method of fungus residue in coniothyrium minitans fermentation technology
CN106035985A (en) * 2016-05-31 2016-10-26 同济大学 Method for producing single cell proteins by using processed waste from mixed bacteria liquid fermentation of yellow wine
CN106578802A (en) * 2016-12-14 2017-04-26 曹书华 Method for preparing fermented beverage by utilizing vinasse

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104256057A (en) * 2014-10-01 2015-01-07 青岛嘉瑞生物技术有限公司 Method for preparing feed proteins by utilizing alcohol waste liquor and crop straws
CN104273378A (en) * 2014-10-10 2015-01-14 青岛嘉瑞生物技术有限公司 New process for producing fermented feed for dairy cows by taking wheat straw and distillers' grains as raw materials
CN104759445A (en) * 2015-03-12 2015-07-08 四川省农业科学院植物保护研究所 Harmless treatment method of fungus residue in coniothyrium minitans fermentation technology
CN106035985A (en) * 2016-05-31 2016-10-26 同济大学 Method for producing single cell proteins by using processed waste from mixed bacteria liquid fermentation of yellow wine
CN106578802A (en) * 2016-12-14 2017-04-26 曹书华 Method for preparing fermented beverage by utilizing vinasse

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Application publication date: 20170926