CN107192697A - A kind of fluorescence sense method for detecting exonuclease I - Google Patents
A kind of fluorescence sense method for detecting exonuclease I Download PDFInfo
- Publication number
- CN107192697A CN107192697A CN201710363009.2A CN201710363009A CN107192697A CN 107192697 A CN107192697 A CN 107192697A CN 201710363009 A CN201710363009 A CN 201710363009A CN 107192697 A CN107192697 A CN 107192697A
- Authority
- CN
- China
- Prior art keywords
- exo
- fluorescence intensity
- iridium
- ccp
- complex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a kind of fluorescence sense method of detection exonuclease I (Exo I), feature is to comprise the following steps that：(1) cation conjugated polymer (CCP) is mixed with complex of iridium, then Exo I buffer solutions are added, the fluorescent optical sensor system based on CCP Yu complex of iridium FRET (FRET) effect for detecting Exo I is obtained.Fluorescence intensity；(2) single stranded DNA (ssDNA) is added to the system in step (1), the change of fluorescence intensity；(3) the Exo I of various concentrations are added in step (2), detect the activity of Exo I in sample, advantage is to have invented that a species specificity is good, sensitivity is high, detection speed is fast for the first time, as a result accurately and reliably detection of the analysis method for sensing for Exo I.
Description
Technical field
The present invention relates to biological sensor, passed more particularly, to a kind of fluorescence of detection exonuclease I (Exo I)
Sensing method.
Background technology
Exonuclease (Exonucleases) is a class sequentially catalytic degradation nucleosides since one of polynucleotide chain
The enzyme of acid.Exonuclease is divided into：Exonuclease I (Exo I) and exonuclease III (Exo III).Exo I are a kind of
It is only capable of from the 3 ' of single stranded DNA (ssDNA) ends to 5 ' ends, according to base sequence, the phosphodiester bond between catalyzing hydrolysis base one by one,
Gradually degrade ssDNA enzyme.After Exo I hydrolysis ssDNA, final product is deoxyribonucleotide one by one.Exo I are to ssDNA
Base sequence do not require, without hydrolysis double-stranded DNA ability.Exonuclease biology it is many during
Play an important role, such as DNA replication dna, restructuring, reparation.Most important of which function is exactly to maintain the gene in organism to dash forward
Become probability, so as to maintain the stability of hereditary information.In recent years, Exo III quantitative detection is reported in succession, but Exo I
Research it is also seldom.
At present to Exo I Activity determination general lack of quantitative description because the method for traditional detection Exo I activity be by
The DNA of standard and Exonucleolytic enzyme reaction, gel electrophoresis observation digestion effect, this method give up to semi-quantitative results, and
And troublesome poeration, poor reproducibility, the Enzyme activities under different condition can not be provided in real time.Therefore, develop a kind of quick, accurate
Really, the method for sensitive quantitatively detection Exo I activity, will have important value.
Complex of iridium has high, the larger Stokes displacements of luminous efficiency, glow color can be by changing ligand structure
It is adjusted and the advantages of good photostability and as the focus of research.Root is it is reported that complex of iridium is in bio-sensing
Application in terms of device is more and more extensive.Cation conjugated polymer (CCP) has as a kind of new water soluble fluorescence molecule
The advantages of molar absorption coefficient is big, fluorescence quantum yield is high.Meanwhile, conjugated polymer has the function of molecular wire, Neng Goushi
The amplification of existing fluorescence signal.FRET (FRET) phenomenon can occur for complex of iridium and CCP.
FRET phenomenons of the invention based on complex of iridium and CCP, build a kind of simple, highly sensitive, quick analysis sensing
Method, for quantitatively detecting Exo I, process and its simple provides a kind of very promising detection means for clinical practice.Mesh
Before, there is not been reported for the fluorescent optical sensor of the FRET phenomenons detection Exo I activity based on complex of iridium and CCP.
The content of the invention
The technical problems to be solved by the invention are to provide that a species specificity is good, sensitivity is high, detection speed is fast, result is accurate
Really reliably detect Exo I fluorescence sense method.
The present invention solve the technical scheme that is used of above-mentioned technical problem for：A kind of detection Exo I fluorescence sense method,
Comprise the following steps that：
(1) it is that 3~4 μM of CCP are that 0.5~2mM complex of iridium is mixed with 0.5~2 μ L concentration by 85~95 μ L concentration, so
Afterwards add respective volume 10 × Exo I buffer solutions, obtain 100 μ L be used for detect Exo I based on CCP and complex of iridium FRET
The fluorescent optical sensor system of effect.Fluorescence intensity, calculates CCP (415nm) fluorescence intensity and complex of iridium (530nm)
Fluorescence intensity ratio.
(2) add 5~15 μM of 1~3 μ L ssDNA arrive the system in step (1), the change of fluorescence intensity, calculating
CCP (415nm) fluorescence intensity and the fluorescence intensity ratio of complex of iridium (530nm).
(3) the Exo I of various concentrations are added in step (2), then add 10 × Exo I buffer solutions of respective volume,
25~35min is incubated at 30~40 DEG C, the change of fluorescence intensity calculates CCP (415nm) fluorescence intensity and complex of iridium
The fluorescence intensity ratio of (530nm), obtains a series of corresponding fluorescence intensity ratios of Exo I of various concentrations, sets up fluorescence strong
The quantitative relationship spent between ratio and Exo I concentration；The activity of Exo I in sample can be detected according to this quantitative relationship.
Using the above-mentioned biological sensor detection Exo I based on complex of iridium and CCP method, fluorescence point is carried out
Analyse, setting slit width is：10nm, excitation wavelength is 380nm, and scanning voltage is 700V, detects CCP/ssDNA/ complex of iridium
System calculates CCP (415nm) fluorescence intensity (I to the Exo I of different activities fluorescence intensity415) and complex of iridium
Fluorescence intensity ratio (the I of (530nm)530)。
Inventive principle：The present invention disturbed based on ssDNA the FRET between CCP and complex of iridium developed it is a kind of it is unmarked,
Simple and easy sensor is used for the activity for detecting Exo I.Due to CCP positively chargeds, lead between the complex of iridium with negative electricity
Cross electrostatic interaction and occur FRET, when adding ssDNA, the fluorescence intensity ratio of the two is reduced；In the presence of having Exo I, due to Exo
I hydrolyzes ssDNA so that the fluorescence intensity ratio increase of the two.Exo I concentration is bigger, the fluorescence intensity ratio I of the two530/I415
It is bigger, it is linear between the logarithm of fluorescence intensity ratio and Exo I concentration.
Compared with prior art, the advantage of the invention is that：The present invention constructs a kind of detection Exo I fluorescence sense side
Method.First, the FRET between CCP and complex of iridium is disturbed using ssDNA.Secondly, in the presence of Exo I, hydrolyzed using Exo I
SsDNA so that I530/I415Increase.Exo I concentration is bigger, the fluorescence intensity ratio I of the two530/I415It is bigger.Experimental result table
It is bright, it is linear between the logarithm of fluorescence intensity ratio and Exo I concentration, realize the detection to Exo I.It the advantage is that：
(1) high sensitivity.In 0.01-2U/mL concentration ranges, I530/I415Between value and the logarithm of Exo I concentration (C)
Good linear relationship is presented, linear equation is I530/I415=1.29+0.45 × 1gC (U/mL), linearly dependent coefficient R2=
0.9926, represent that curve has good degree of fitting, detection is limited to 0.01U/mL.These results indicate that the sensor is to Exo I
Detection there is relatively low test limit and higher sensitivity.
(2) high specific.Exonuclease (Exo III), limitation restriction endonuclease (Hind III), exonuclease (λ-
Exo), limitation restriction endonuclease (EcoR I), as control substance of plant drug, is 0.1U/mL, is detected noiseless.
(3) result is accurate.In 10% urine and 10% serum environment, the rate of recovery is between 90%~110%.
(4) prepare with detection method reagent dosage is few, detection speed is fast, cost is low.The present invention only need to consume a small amount of material
The highly sensitive detection to Exo I is achieved that with reagent.
In summary, the present invention be disturbed based on ssDNA the FRET between CCP and complex of iridium build it is a kind of it is unmarked,
Simple and easy sensor is used to detect Exo I activity, with sensitivity is high, selectivity is good, simple to operate, analysis is quick,
Easily operated the advantages of, it is possible to achieve low concentration Exo I detection, have a good application prospect.
Brief description of the drawings
Fig. 1 is the feasibility Experiment figure of inventive sensor；
Fig. 2 is fluorescence response figure of the inventive sensor to various concentrations Exo I；
Fig. 3 is calibration graph of the inventive sensor to various concentrations Exo I；
Fig. 4 is selective lab diagram of the inventive sensor to Exo I；
Fig. 5 is interference--free experiments figure of the inventive sensor to Exo I.
Embodiment
The present invention is described in further detail below in conjunction with accompanying drawing embodiment.
First, specific embodiment
Embodiment 1
The present invention solve the technical scheme that is used of above-mentioned technical problem for：A kind of detection Exo I fluorescence sense method,
Comprise the following steps that：
(1) it is that 3.28 μM of CCP are that 1mM complex of iridium is mixed with 1 μ L concentration by 90 μ L concentration, then adds respective volume
10 × Exo I buffer solutions, obtaining 100 μ L is used to detect the Exo I fluorescence sense based on CCP Yu complex of iridium FRET effects
Body system.Fluorescence intensity, calculates CCP (415nm) fluorescence intensity and the fluorescence intensity ratio of complex of iridium (530nm).
(2) add 10 μM of 2 μ L ssDNA arrive the system in step (1), the change of fluorescence intensity, calculating CCP
The fluorescence intensity of (415nm) and the fluorescence intensity ratio of complex of iridium (530nm).
(3) the Exo I of various concentrations are added in step (2), then 10 × Exo I buffer solutions of respective volume, 37 DEG C
Lower incubation 30min, the change of fluorescence intensity, calculate CCP (415nm) fluorescence intensity and complex of iridium (530nm) it is glimmering
Intensity ratio, obtains a series of corresponding fluorescence intensity ratios of Exo I of various concentrations, sets up fluorescence intensity ratio and Exo
Quantitative relationship between I concentration；The activity of Exo I in sample can be detected according to this quantitative relationship.
Embodiment 2
The present invention solve the technical scheme that is used of above-mentioned technical problem for：A kind of detection Exo I fluorescence sense method,
Comprise the following steps that：
(1) it is that 4 μM of CCP are that 1mM complex of iridium is mixed with 2 μ L concentration by 85 μ L concentration, then adds the 10 of respective volume
× Exo I buffer solutions, obtain the fluorescence sense body based on CCP Yu complex of iridium FRET effects that 100 μ L are used to detect Exo I
System.Fluorescence intensity, calculates CCP (415nm) fluorescence intensity and the fluorescence intensity ratio of complex of iridium (530nm).
(2) add 15 μM of 1 μ L ssDNA arrive the system in step (1), the change of fluorescence intensity, calculating CCP
The fluorescence intensity of (415nm) and the fluorescence intensity ratio of complex of iridium (530nm).
(3) the Exo I of various concentrations are added in step (2), then 10 × Exo I buffer solutions of respective volume, 34 DEG C
Lower incubation 30min, the change of fluorescence intensity, calculate CCP (415nm) fluorescence intensity and complex of iridium (530nm) it is glimmering
Intensity ratio, obtains a series of corresponding fluorescence intensity ratios of Exo I of various concentrations, sets up fluorescence intensity ratio and Exo
Quantitative relationship between I concentration；The activity of Exo I in sample can be detected according to this quantitative relationship.
Embodiment 3
The present invention solve the technical scheme that is used of above-mentioned technical problem for：A kind of detection Exo I fluorescence sense method,
Comprise the following steps that：
(1) it is that 3 μM of CCP are that 2mM complex of iridium is mixed with 0.5 μ L concentration by 95 μ L concentration, then adds respective volume
10 × Exo I buffer solutions, obtain the fluorescent optical sensor based on CCP Yu complex of iridium FRET effects that 100 μ L are used to detect Exo I
System.Fluorescence intensity, calculates CCP (415nm) fluorescence intensity and the fluorescence intensity ratio of complex of iridium (530nm).
(2) add 5 μM of 3 μ L ssDNA arrive the system in step (1), the change of fluorescence intensity, calculating CCP
The fluorescence intensity of (415nm) and the fluorescence intensity ratio of complex of iridium (530nm).
(3) the Exo I of various concentrations are added in step (2), then 10 × Exo I buffer solutions of respective volume, 32 DEG C
Lower incubation 30min, the change of fluorescence intensity, calculate CCP (415nm) fluorescence intensity and complex of iridium (530nm) it is glimmering
Intensity ratio, obtains a series of corresponding fluorescence intensity ratios of Exo I of various concentrations, sets up fluorescence intensity ratio and Exo
Quantitative relationship between I concentration；The activity of Exo I in sample can be detected according to this quantitative relationship.
2nd, feasibility Experiment
Determine the fluorescence intensity of CCP/ complex of iridium systems；The ssDNA composition CCP/ssDNA/ iridium for adding 66 bases is matched somebody with somebody
Compound system, determines its fluorescence intensity；Add and 30min is cultivated at Exo I (10U/mL, 1 μ L), 37 DEG C, determine the fluorescence of system
Intensity.Exo I (10U/mL, 1 μ L) are added in the only system of CCP/ complex of iridium in addition, control experiment is used as.Together
Shi Liyong chemiluminescence fluorescence imagings system obtains the fluoroscopic image (specific implementation example 1) of each solution.When exist in system CCP and
Efficient FRET occurs during complex of iridium；Add after ssDNA, FRET efficiency is strongly reduced；Due to being hydrolyzed in the presence of Exo I
FRET (such as Fig. 1) occurs again for CCP and complex of iridium in the fluorescence intensity enhancing at ssDNA, 530nm, system.So we
The fluorescent optical sensor based on CCP and complex of iridium FRET effects of design can detect Exo I activity, and can quantify detection
Exo I concentration, there is certain application potential.
3rd, Exo I detections application
1st, the method that the biological sensor prepared using above-mentioned specific embodiment 1 detects Exo I
Using fluorescence analysis, setting slit width is：10nm, excitation wavelength is 380nm, and scanning voltage is 700V, detection
CCP (415nm) fluorescence intensity and the fluorescence intensity ratio of complex of iridium (530nm) in CCP/ssDNA/ complex of iridium systems
I530/I415To various concentrations Exo I quantitative relationship, according to quantitative relationship between the two, Exo I in testing sample are determined
Content (specific implementation example 1).Fluorescence intensities of the Fig. 2 at 530nm strengthens with the increase of Exo I concentration, and this means that
With complex of iridium efficient FRET occurs again for CCP, therefore FRET efficiency increases.
2nd, sensitivity test
Using fluorescence analysis, setting slit width is：10nm, excitation wavelength is 380nm, and scanning voltage is 700V, above-mentioned
The fluorescence intensity ratio I of CCP/ssDNA/ complex of iridium systems prepared by specific embodiment 1530/I415, the scope of Exo I concentration
For 0~2U/mL (specific implementation example 1).Result of the test illustrates, as shown in figure 3, explanation is with the increase of Exo I concentration, I530/
I415It is more obvious；Sensor I530/I415Linear correlation equation to the logarithm of Exo I concentration is I530/I415=1.29+0.45 ×
IgC (U/mL), R2=0.9926, the range of linearity is 0.01~2.0U/mL, is calculated and learnt according to S/N, and detection is limited to 0.01U/
mL.Illustrate that sensor can realize high-sensitivity detection to Exo I.
3rd, specificity experiments
With interference--free experiments as shown in Figures 4 and 5, the concentration of wherein Exo I and other enzymes is 0.1U/ for selectivity experiment
ML, other used enzymes are as follows：Exonuclease (Exo III), limitation restriction endonuclease (Hind III), exonuclease (λ-
Exo), limitation restriction endonuclease (EcoR I).
(1) selectivity experiment
Using fluorescence analysis, setting slit width is：10nm, excitation wavelength is 380nm, and scanning voltage is 700V, above-mentioned
Exonuclease (the Exo that CCP/ssDNA/ complex of iridium system difference detectable concentration prepared by specific embodiment 1 is 0.1U/mL
III), limitation restriction endonuclease (Hind III), exonuclease (λ-Exo), limitation restriction endonuclease (EcoR I).Such as Fig. 4, with Exo I
Contrast, response of the sensor to other enzymes is very small, substantially close to blank signal, illustrates that sensor has for Exo I detection
It is selective well.
(2) interference--free experiments,
Using fluorescence analysis, setting slit width is：10nm, excitation wavelength is 380nm, and scanning voltage is 700V, above-mentioned
CCP/ssDNA/ complex of iridium systems prepared by specific embodiment 1, it is 0.1U/mL that detectable concentration is distinguished in the presence of 0.1U/mL
Exonuclease (Exo III), limitation restriction endonuclease (Hind III), exonuclease (λ-Exo), limitation restriction endonuclease (EcoR
I), sensor is compared to the fluorescence response in the presence of four systems and only Exo I, such as Fig. 5, it was observed that I530/I415Size with
I in the presence of only Exo I530/I415Substantially without difference, illustrate that sensor realizes the specific detection to Exo I.
Certainly, described above not limitation of the present invention, the present invention is also not limited to the example above.The art
The variations, modifications, additions or substitutions that those of ordinary skill makes in the essential scope of the present invention, should also belong to protection of the present invention
Scope.
Claims (3)
1. one kind detection exonuclease I (Exo I) fluorescence sense method, is comprised the following steps that：
(1) it is 3~4 μM of cation conjugated polymers (CCP) by 85~95 μ L concentration and 0.5~2 μ L concentration is 0.5~2mM iridium
Complex mix, then add respective volume 10 × Exo I buffer solutions, obtain 100 μ L be used for detect Exo I based on sun from
Sub- conjugated polymer (CCP) and the fluorescent optical sensor system of complex of iridium FRET (FRET) effect.Detection is glimmering
Luminous intensity, calculates CCP (415nm) fluorescence intensity and the fluorescence intensity ratio of complex of iridium (530nm).
(2) add 5~15 μM of 1~3 μ L ssDNA arrive the system in step (1), the change of fluorescence intensity, calculating CCP
The fluorescence intensity of (415nm) and the fluorescence intensity ratio of complex of iridium (530nm).
(3) the Exo I of various concentrations are added in step (2), then 10 × Exo I buffer solutions of respective volume, 30~40 DEG C
25~35min of lower incubation, the change of fluorescence intensity calculates CCP (415nm) fluorescence intensity and complex of iridium (530nm)
Fluorescence intensity ratio, obtain a series of corresponding fluorescence intensity ratio I of Exo I of various concentrations530/I415, set up fluorescence strong
Spend ratio I530/I415With the quantitative relationship between Exo I concentration；Exo I in sample can be detected according to this quantitative relationship
Activity.
2. a kind of detection Exo I according to claim 1 fluorescence sense method, it is characterised in that：Exo I analysis is passed
Sense detection report is less, realizes Exo I detection using the FRET between complex of iridium and CCP for the first time.
3. one kind utilizes the fluorescence sense method of detection Exo I described in claim 1- (3) a kind of, it is characterised in that：It is narrow
Slit width degree is：10nm, excitation wavelength is 380nm, and scanning voltage is 700V, CCP in detection CCP/ssDNA/ complex of iridium systems
Fluorescence intensity (the I of (415nm)415) with the fluorescence intensity ratio I of complex of iridium (530nm)530/I415To various concentrations Exo I's
Quantitative relationship.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710363009.2A CN107192697B (en) | 2017-05-08 | 2017-05-08 | A kind of fluorescence sense method detecting exonuclease I |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710363009.2A CN107192697B (en) | 2017-05-08 | 2017-05-08 | A kind of fluorescence sense method detecting exonuclease I |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107192697A true CN107192697A (en) | 2017-09-22 |
| CN107192697B CN107192697B (en) | 2019-09-27 |
Family
ID=59874267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710363009.2A Active CN107192697B (en) | 2017-05-08 | 2017-05-08 | A kind of fluorescence sense method detecting exonuclease I |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107192697B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108085368A (en) * | 2017-11-23 | 2018-05-29 | 南京邮电大学 | A kind of method based on water-soluble cationic conjugated polymer material detection DNA |
-
2017
- 2017-05-08 CN CN201710363009.2A patent/CN107192697B/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108085368A (en) * | 2017-11-23 | 2018-05-29 | 南京邮电大学 | A kind of method based on water-soluble cationic conjugated polymer material detection DNA |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107192697B (en) | 2019-09-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20050112601A1 (en) | Methods for cellular or microorganism capture and quantification using bioluminescence regenerative cycle (BRC) assays | |
| EP3830291A1 (en) | Specific detection of deoxyribonucleic acid sequences using novel crispr enzyme-mediated detection strategies | |
| US20220106647A1 (en) | Solution-phase, trans-activated reporter systems for use in crispr-based nucleic acid sequence detections | |
| US9777313B2 (en) | Silver nanocluster probe and target polynucleotide detection method using same, and silver nanocluster probe design method | |
| Ma et al. | CRISPR/Cas12a system responsive DNA hydrogel for label-free detection of non-glucose targets with a portable personal glucose meter | |
| CN104034782A (en) | Potential sensor for detecting dichlorotris (1,10-phenanthroline) ruthenium (II) and detection method and application of potential sensor | |
| CN106568820A (en) | Preparation method for synthesizing silver nanocluster electrochemical biosensor based on DNA signal amplification technique and application of electrochemical biosensor | |
| Pan et al. | Label-free and highly sensitive fluorescence detection of lead (ii) based on DNAzyme and exonuclease III-assisted cascade signal amplification | |
| Zhang et al. | A highly sensitive LED-induced chemiluminescence platform for aptasensing of platelet-derived growth factor | |
| Ye et al. | Sensitive detection of alkaline phosphatase based on terminal deoxynucleotidyl transferase and endonuclease IV-assisted exponential signal amplification | |
| Zhang et al. | Peptide-modified nanochannel system for carboxypeptidase B activity detection | |
| CN107561045A (en) | A kind of fluorescence micro DNA detection method | |
| Wang et al. | Glucometer-based electrochemical biosensor for determination of microRNA (let-7a) using magnetic-assisted extraction and supersandwich signal amplification | |
| Zhang et al. | Recording the Reaction Process of Loop‐Mediated Isothermal Amplification (LAMP) by Monitoring the Voltammetric Response of 2′‐Deoxyguanosine 5′‐Triphosphate | |
| CN109613096A (en) | DNA relevant enzyme electrochemica biological sensor and its application based on the building of DNA- copper nano-cluster | |
| CN107083437B (en) | Method for simultaneously detecting multiple DNA glycosylases by using inherent fluorescent nucleotide ultrasensitiveness | |
| EP3036341B1 (en) | Detection of nucleic acid amplification in a porous substrate | |
| CN107192697A (en) | A kind of fluorescence sense method for detecting exonuclease I | |
| Li et al. | Immobilization enzyme fluorescence capillary analysis for determination of lactic acid | |
| Lin et al. | Detection of BCR–ABL using one step reverse transcriptase-polymerase chain reaction and microchip electrophoresis | |
| CN106706578A (en) | Fluorescence detection method for hydrolase activity | |
| Lin et al. | Microfluidic chip-based microbial metabolism-indexed BOD sensor for rapid determination of biochemical oxygen demand | |
| Yang et al. | A split-type electrochemical biosensor using enzyme-linked DNA magnetic beads realizes the detection of BCR/ABLp210 fusion gene in clinical samples: Duplex ligation chain reaction coupled with OR logic gate design | |
| Zhang et al. | A CRISPR/Cas12a-assisted array for Helicobacter pylori DNA analysis in saliva | |
| CN109097449A (en) | A kind of real-time fluorescence LAMP detection method and kit based on ruthenium complex |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221207 Address after: Room 521-73, Floor 5, No. 23, Lane 10688, Beiqing Road, Qingpu District, Shanghai, 200000 Patentee after: Shanghai Baichuangyi Biotechnology Co.,Ltd. Address before: Room 2202, 22 / F, Wantong building, No. 3002, Sungang East Road, Sungang street, Luohu District, Shenzhen City, Guangdong Province Patentee before: Shenzhen dragon totem technology achievement transformation Co.,Ltd. Effective date of registration: 20221207 Address after: Room 2202, 22 / F, Wantong building, No. 3002, Sungang East Road, Sungang street, Luohu District, Shenzhen City, Guangdong Province Patentee after: Shenzhen dragon totem technology achievement transformation Co.,Ltd. Address before: 315211 Ningbo University, 818 Fenghua Road, Jiangbei District, Ningbo, Zhejiang Patentee before: Ningbo University |