CN107189999A - A kind of preparation method and applications of the plain carrier of Magnetic agglutination - Google Patents
A kind of preparation method and applications of the plain carrier of Magnetic agglutination Download PDFInfo
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- CN107189999A CN107189999A CN201710342740.7A CN201710342740A CN107189999A CN 107189999 A CN107189999 A CN 107189999A CN 201710342740 A CN201710342740 A CN 201710342740A CN 107189999 A CN107189999 A CN 107189999A
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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Abstract
The invention belongs to full cell fixation field, the method for disclosing a kind of preparation of the plain carrier of Magnetic agglutination and being applied in full cell high-efficient immobilization.The key of technical solution of the present invention is to modify magnetic nano-particle by dopamine hydrochloride, the quinonyl and amino functional group carried by surface and agglutinin covalent bond.The carrier surface area is larger, and preparation method is simple.It is full cell present invention employs restructuring E.coli, Magnetic agglutination element is used for immobilization for carrier, and fixed rate is up to 80 90%.Therefore, prepare the plain carrier of the Magnetic agglutination and provide new thinking and with certain Research Significance for the method for immobilized whole-cell to study efficient immobilization.
Description
Technical field
The invention belongs to full cell fixation field, it is related to a kind of preparation of the plain carrier of Magnetic agglutination and for the fast of bacterium
Fast immobilization.
Background technology
ConA is a kind of phytohemagglutinin, its essence be a class multivalence, nonimmune source, to sugared and its conjugated
The protein of the highly single-minded identification of thing.ConA is used primarily for immunological investigation, is ground with the development and property of ConA purification process
That studies carefully is increasingly deep, and the scope of ConA applications is also more and more wider.Except outer applied to clinical diagnosis, treatment, ConA turned into it is chemical,
Useful research tool in the fields such as biochemistry, biology, immunology and medical science.Ana Cecilia Alves (referring to：
Ana.C.A.et al, 2015) it is prepared into a kind of vasodilator using chitosan beads immobilization agglutinin；Oriol Penin
(referring to：Oriol.P.et al, 2014) agglutinin is fixed on polysilicon surface it is used for cell detection；Pan yiting (ginsengs
See：Pan e t al, 2015) will fixed agglutinin in the enrichment for being used to glycoprotein on polymer；Alex Monzo (referring to：
Alex et al, 2007) immobilization agglutinin is used to be enriched with glycopolymers in agarose and silica surface.
Bacterium is a kind of important biocatalyst, have been widely used for medicine, pharmaceutical intermediate and food preparation,
In the industrial production such as sewage and atmospheric treatment, chemical industry light industrial goods (referring to：de Carvalho,2011).But free cell is in training
Support unstable in base or reaction solution, be difficult continuously to use, and have to reaction environment higher requirement (referring to：Dubey et
al.,2013).Stable immobilized cell is obtained by immobilization, then can be achieved to reuse, and can in bioreactor
High cell concentration is obtained, use cost is reduced, easily reclaims, simple to operate (referring to：Anisha and Prema,2008；
Leonard et al.,2011).For recombinant bacterium, using immobilized cell, without will again be consolidated after enzyme purification
Fixedization, and intracellular has the various redox enzymes needed for cofactor regeneration, for oxidoreducing enzyme, without add it is auxiliary because
Son, substantially reduce biocatalyst preparation cost (referring to：Liet al.,2013；Zajkoska et al.,2013).Mesh
Before, many scholars both domestic and external have carried out research in immobilization bacterium this aspect and attempted.For example, the Ni Ke of East China University of Science
Give (referring to：Kefeng Ni et al, 2012) it is prepared for a kind of bionic adhesion material fixation in situ Gluconobacter oxydans
Bacillus；Gustav Rehn (referring to：Gustav R.et al, 2013) etc. it is big using the flocculation immobilization restructuring of chitosan
Enterobacteria；Nadia Touisui (referring to：Nadia T.et al, 2013) it is prepared for a kind of magnesium aluminum double hydroxide immobilization weight
Group Escherichia coli.Based on background above, the present invention draws up for a kind of plain carrier of Magnetic agglutination, big by magnetic Nano material surface
The functional groups such as the quinonyl of amount are combined with the amino covalence of agglutinin, prepare this magnetic carrier.Using agglutinin to cell
Agglutination immobilization recombination bacillus coli.
The content of the invention
It is an object of the invention to provide a kind of method of quick immobilization recombination bacillus coli, greatly shortened admittedly with reaching
Surely change the time, this process for fixation is applied widely, the biocatalytic Activity of cell is not influenceed, resistance to mass tranfer is small, and prepare
Immobilized cell easily recycle.
It is a further object of the present invention to provide the preparation method of this carrier for quick immobilization.
To achieve the above object, the technical solution adopted by the present invention is as follows：
(1) by FeSO4·4H2O and FeCl3·6H2O is according to mol ratio 1:1~1:5 are dissolved in distilled water, are then transferred to
In three neck round bottom, at 40~80 DEG C, under stirring and nitrogen protective condition, 10-40% NH is rapidly added4OH adjusts solution
To alkalescence, reaction 5-20min after, add oleic acid, be stirred for reaction 25-50min after, the black precipitate absolute ethyl alcohol of acquisition and
Ultra-pure water is cleaned to supernatant water white transparency, strong magnet precipitation separation repeatedly, is dried in vacuum drying oven, is obtained oleic acid magnetic and receive
Rice grain is referred to as Fe3O4@OA；
(2) weigh a certain amount of dopamine hydrochloride to be dissolved in cushioning liquid, be configured to 1~10mg/mL dopamine
Hydrochloric acid solution, oleic acid magnetic nanoparticle grinding prepared by step (1) is even added in dopamine hydrochloric acid solution, ultrasound 10
~50min, is then empty in gas 7~12h of stirring, after stirring terminates, is separated with magnet, the dark brown deposit ethanol of acquisition and
Distilled water is cleaned, and 50 DEG C of drying, obtain magnetic nanoparticle and be referred to as Dopamine magnetic nano-carrier in vacuum drying oven,
It is designated as Fe3O4@OA@DP；
(3) ConA conA is dissolved in reference in cushioning liquid, obtains certain density ConA conA
Storing solution, takes certain density storing solution, is added to the Fe prepared in step (2)3O4Reacted in@OA@DP nano-particles,
Reaction is centrifuged off supernatant after terminating, and obtains Fe3O4@OA@DP@conA magnetic carriers.
In step (1), the oleic acid volume is the 0.1~1% of total solution volume.
In step (2), the concentration of the dopamine hydrochloric acid solution is 0.5-3mg/mL；Buffer solution is that Citrate buffer is molten
Liquid, phosphate buffer solution or Tris-HCl salt buffer solutions, pH is 4.0-9.0.
In step (2), ultrasonic time is 20~40min before the reaction.
In step (1) and step (2), drying temperature is 50 DEG C in vacuum drying oven.
In step (3), the ConA conA Stock concentrations are 0.5-5g/L；Combination buffer is to contain 1mM
Ca2+With 1mM Mn2+Citrate buffer solution, phosphate buffer solution or Tris-HCl salt buffer solutions, pH is 4.0-
9.0。
In step (3), the reaction temperature is 4-25 DEG C, and the reaction time is 1-8h.
The plain carrier of Magnetic agglutination that described preparation method is prepared, is applied to the immobilization of full cell.
Concrete application method is：
Cell is suspended in and combined in cushioning liquid, and measures initial OD600Value, 1-8mL cell suspending liquid is added to
10~30mg Fe of above-mentioned preparation3O4In@OA@DP@conA magnetic carriers, 0.5-6h is reacted at 0-25 DEG C, strong magnet is separated,
Supernatant OD values are measured, cleans 2-3 times, measures supernatant OD values, and calculate immobilization efficiency.
The method that the plain carrier of described Magnetic agglutination is applied to full cell fixation, the full cell is recombination bacillus coli
Cell, the combination buffer is to contain 1mM Ca2+With 1mM Mn2+Citrate buffer solution, phosphate buffer solution or
Person's Tris-HCl salt buffer solutions, pH is 4.0-9.0.
Beneficial effects of the present invention
The present invention has following beneficial effect：
1st, the present invention substantially reduces the immobilization time of cell；
2nd, invention increases the swing free degree of cell on carrier, reduce due to the space bit that macromolecular carrier is caused
Resistance；
3rd, the present invention improves the efficiency of cell, enhances the heat endurance of cell, to the tolerance of acid, extends and partly declines
Phase, it is easy to the separation and recycling of cell, and it is simple to operate.
Brief description of the drawings：
Fig. 1 is the magnetic nano-particle Fe prepared3O4@OA、Fe3O4@OA@DP transmission electron microscope picture.
Fig. 2 is the Fe prepared3O4@OA and Fe3O4@OA@DP infrared analysis figure
Fig. 3 is that embodiment fixes rate pH Optimal Curve figures.
Fig. 4 is embodiment enzyme activity pH Optimal Curve figures.
Embodiment
Embodiment set forth below contributes to those skilled in the art to more fully understand the present invention, but does not limit in any way
The present invention.
Key instrument used in following examples and material
Experiment material：Coomassie brilliant G-250, sodium dihydrogen phosphate, disodium hydrogen phosphate, trishydroxymethylaminomethane, a water
Citric acid, sodium citrate, iron chloride (FeCl3·6H2O), ferrous sulfate (FeSO4·4H2O), oleic acid, absolute ethyl alcohol, hexichol
Amine, dihydroxyacetone (DHA), the concentrated sulfuric acid, acetic acid, dopamine hydrochloride, ConA (conA).
Laboratory apparatus：Digital display thermostat water bath (HH-S, Medical Instruments factory of Jintan City of Jiangsu Province), UV, visible light spectrophotometric
Count (UV-1800, Shanghai Mei Puda Instrument Ltd.), quick vortex mixer (SK-1, Community of Jin Tan County Zhong great instrument plant), four-dimensional rotation
Turn vortex mixer (BE-1100, its woods Bel Instrument Ltd. of Haimen City), vacuum desiccator (DZF-6020, the permanent science and technology in Shanghai one
Instrument Ltd.), Fourier transformation infrared spectrometer (Spectrum One-B, Shimadzu Corp), superclean bench
(SW-CJ-2FD, Purifying Equipment Co., Ltd., Suzhou).
The optimization of the immobilization recombination bacillus coli immobilization pH of embodiment 1
Weigh 6.0g FeSO4·4H2O and 11.6g FeCl3·6H2O is dissolved in 350mL distilled water, is then transferred to three
In neck round-bottom flask, under 80 DEG C, high-speed stirred and nitrogen protective condition, 20mL 25%NH are rapidly added4OH.React 5min
Afterwards, 1.0mL oleic acid is added.The black precipitate obtained after stirring reaction 25min cleans supreme repeatedly with absolute ethyl alcohol and ultra-pure water
Clear liquid water white transparency, strong magnet precipitation separation, 50 DEG C of drying, obtain oleic acid magnetic nanoparticle and are referred to as in vacuum drying oven
Fe3O4@OA。
The dopamine hydrochloride for weighing 0.2g is dissolved in 10mM pH 8.5 Tris-HCl solution, is configured to 2mg/mL
Dopamine solution.The oleic acid magnetic nano-particle grinding of the above-mentioned preparations of 1.0g is even added in dopamine hydrochloric acid solution,
Ultrasonic 15min, is then empty in gas quick stirring 12h.After stirring terminates, separated with magnet, the dark brown deposit second of acquisition
Alcohol and distilled water are cleaned 4 times, and 50 DEG C of drying, obtain magnetic nano-particle referred to as Fe in vacuum drying oven3O4@OA@DP。
Weigh 30mg Fe3O4@OA@DP nano materials, add 0.3mL (2mg/mL) ConA storing solution, 0.7mL knots
Buffer solution is closed, 2h is reacted under the conditions of 7.4,4 DEG C of pH, after reaction terminates, centrifugation takes supernatant 0.8mL, solid precipitation phosphorus
Hydrochlorate cushioning liquid (0.01M, pH 7.4) is cleaned 2 times, and supernatant 0.8mL is taken respectively, is determined respectively 3 times with Bradford methods
The content of protein in supernatant, obtains magnetic Nano material referred to as Fe3O4@OA@DP@ConA。
Fig. 1 is the magnetic nano-particle Fe prepared3O4@OA and Fe3O4@OA@DP TEM figures.Figure A is magnetic Fe3O4@OA receive
Rice corpuscles, figure B is magnetic Fe3O4@OA@DP nano-particles.As can be seen from Figure magnetic nano-particle through DOPA it is amine-modified after, particle diameter
It has been increased that, about 20nm, and dopamine from poly- oxidation make its carrier surface formation one tunic, by magnetic Nano
Grain parcel wherein, makes nano-particle show slight clustering phenomena.
Fig. 2 is the magnetic Fe prepared3O4@OA and Fe3O4The infrared analysis figure of@OA@DP nano-particles.In magnetic Fe3O4@OA
In particle infrared absorption peak, in 1409,1523,2852 and 2922cm-1The peak that place occurs is-CH in oleic acid respectively3, C=O and
CH2Absworption peak.Fe3O4@OA through DOPA it is amine-modified after, in 2852,2922 and 1523cm-1The absworption peak at place is by one in 1440-
Broad peak in the range of 1605cm-1 is replaced, and illustrates that dopamine is wrapped in carrier surface by being reacted with oleic acid.
14 parts of functional magnetic nano materials are weighed, every part of 30mg adds 2mL bacterial suspension (9mg/ml), suspended
The pH of liquid is respectively that 2h is reacted at 4,5,6,7.4,8,9,10,4 DEG C, after reaction terminates, and supernatant OD is surveyed in ferromagnetism separation600Value, is adopted
With (initial bacteria suspension OD600- supernatant OD600)/initial bacteria suspension OD600Calculate fixed rate.It is above-mentioned with diphenylamines determination of color
The enzyme activity of immobilization recombination bacillus coli.
As shown in figure 3, under different pH, Fe3O4The effect that@OA@DP@ConA fix recombination bacillus coli shows difference.
PH is at 7.4, fixed rate highest, in the range of pH 4.0-6.0, and fixed rate declines therewith with increasing for pH, this be by
It is pH 5.0 in the isoelectric point of agglutinin, pH rise causes electrostatic adsorption to decline, when pH is increased under alkalescence state
When, agglutinin is strengthened the sugared composition recognition capability of bacterium surface, and fixed rate is significantly raised, in the range of pH8-10, due to
Strong basicity causes conA to inactivate, and fixed rate declines.
As shown in figure 4, under different pH, the enzymatic activity of free recombination bacillus coli shows marked difference, and optimal pH is
9.0, in the range of pH 6.0-9.0, relative activity is more than 70%, but under basic conditions, and activity is remarkably decreased,
During pH11.0, activity decrease to 14.5%；Immobilization recombination bacillus coli enzyme activity is stablized relatively, and optimal pH is 8.0, when pH rises to
When 11.0, activity also retains more than 50%.
Claims (9)
1. the preparation method of the plain carrier of a kind of Magnetic agglutination, it is characterised in that comprise the following steps：
(1) by FeSO4·4H2O and FeCl3·6H2O is according to mol ratio 1:1~1:5 are dissolved in distilled water, are then transferred to three necks
In round-bottomed flask, at 40~80 DEG C, under stirring and nitrogen protective condition, 10-40% NH is rapidly added4OH adjusts solution to alkali
Property, after reaction 5-20min, oleic acid is added, is stirred for after reaction 25-50min, the black precipitate absolute ethyl alcohol of acquisition and ultrapure
Water is cleaned to supernatant water white transparency, strong magnet precipitation separation repeatedly, is dried in vacuum drying oven, is obtained oleic acid magnetic Nano
Grain is referred to as Fe3O4@OA；
(2) weigh a certain amount of dopamine hydrochloride to be dissolved in cushioning liquid, be configured to 1~10mg/mL dopamine hydrochloric acid
Solution, oleic acid magnetic nanoparticle grinding prepared by step (1) is even added in dopamine hydrochloric acid solution, and ultrasound 10~
50min, is then empty 7~12h of stirring in gas, after stirring terminates, is separated with magnet, the dark brown deposit ethanol of acquisition and steaming
Distilled water is cleaned, and is dried in vacuum drying oven, is obtained magnetic nanoparticle and be referred to as Dopamine magnetic nano-carrier, be designated as
Fe3O4@OA@DP；
(3) ConA conA is dissolved in and combined in cushioning liquid, obtain certain density ConA conA deposits
Liquid, takes certain density storing solution, is added to the Fe prepared in step (2)3O4Reacted, reacted in@OA@DP nano-particles
Supernatant is centrifuged off after end, Fe is obtained3O4@OA@DP@conA magnetic carriers.
2. preparation method as claimed in claim 1, it is characterised in that in step (1), the oleic acid volume is total solution volume
0.1~1%.3. preparation method as claimed in claim 1, it is characterised in that in step (2), the dopamine hydrochloric acid is molten
The concentration of liquid is 0.5-3.0mg/mL；Buffer solution is citrate buffer solution, phosphate buffer solution or Tris-HCl salt
Cushioning liquid, pH is 4.0-9.0.
3. preparation method as claimed in claim 1, it is characterised in that in step (2), before the reaction ultrasonic time be 20~
40min。
4. preparation method as claimed in claim 1, it is characterised in that in step (1) and step (2), is dried in vacuum drying oven
Temperature is 50 DEG C.
5. preparation method as claimed in claim 1, it is characterised in that in step (3), the ConA conA storing solutions
Concentration is 0.5-5g/L；Combination buffer is to contain 1mM Ca2+With 1mM Mn2+Citrate buffer solution, phosphate-buffered
Solution or Tris-HCl salt buffer solutions, pH is 4.0-9.0.
6. preparation method as claimed in claim 1, it is characterised in that in step (3), the reaction temperature is 4-25 DEG C, instead
It is 1-8h between seasonable.
7. the plain carrier of Magnetic agglutination that the preparation method as described in claim 1~6 is prepared, is applied to full cell
Immobilization.
8. the method that the plain carrier of Magnetic agglutination according to claim 7 is applied to full cell fixation, it is characterised in that
Concrete application method is：
Cell is suspended in and combined in cushioning liquid, and measures initial OD600Value, 1-8mL cell suspending liquid is added to above-mentioned
10~30mg the Fe prepared3O4In@OA@DP@conA magnetic carriers, 0.5-6h, strong magnet separation, measurement are reacted at 0-25 DEG C
Supernatant OD values, clean 2-3 times, measure supernatant OD values, and calculate immobilization efficiency.
9. the method that the plain carrier of Magnetic agglutination as claimed in claim 8 is applied to full cell fixation, it is characterised in that described
Full cell is recombinant Bacillus coli cells, and the combination buffer is to contain 1mM Ca2+With 1mM Mn2+Citrate buffer
Solution, phosphate buffer solution or Tris-HCl salt buffer solutions, pH is 4.0-9.0.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109355279A (en) * | 2018-12-15 | 2019-02-19 | 大连民族大学 | A kind of mannose magnetic nano-carrier and preparation method thereof |
| CN109593752A (en) * | 2018-12-15 | 2019-04-09 | 大连民族大学 | A kind of method of mannose immobilized cell |
| CN110808137A (en) * | 2019-11-13 | 2020-02-18 | 山东师范大学 | Magnetic enrichment material, water body bacterium detection kit and application |
| CN111921511A (en) * | 2020-07-03 | 2020-11-13 | 西安交通大学 | Hydrogel-based cell stationary phase preparation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105551704A (en) * | 2015-12-09 | 2016-05-04 | 江苏大学 | Preparation and application of dopamine functional magnetic nano-carrier |
| CN106093021A (en) * | 2016-06-03 | 2016-11-09 | 浙江省农业科学院 | The escherichia coli visualization bio-sensing method of acidity regulation and control and agglutinin identification |
-
2017
- 2017-05-16 CN CN201710342740.7A patent/CN107189999A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105551704A (en) * | 2015-12-09 | 2016-05-04 | 江苏大学 | Preparation and application of dopamine functional magnetic nano-carrier |
| CN106093021A (en) * | 2016-06-03 | 2016-11-09 | 浙江省农业科学院 | The escherichia coli visualization bio-sensing method of acidity regulation and control and agglutinin identification |
Non-Patent Citations (2)
| Title |
|---|
| GANGLONG YANG等: "Isolation and identification of native membrane glycoproteins from living cell by concanavalin A–magnetic particle conjugates", 《ANALYTICAL BIOCHEMISTRY》 * |
| MENG-YAO ZHUANG等: "Using concanavalinA as a spacer for immobilization of E. coli ontomagnetic nanoparticles", 《INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109355279A (en) * | 2018-12-15 | 2019-02-19 | 大连民族大学 | A kind of mannose magnetic nano-carrier and preparation method thereof |
| CN109593752A (en) * | 2018-12-15 | 2019-04-09 | 大连民族大学 | A kind of method of mannose immobilized cell |
| CN110808137A (en) * | 2019-11-13 | 2020-02-18 | 山东师范大学 | Magnetic enrichment material, water body bacterium detection kit and application |
| CN110808137B (en) * | 2019-11-13 | 2021-08-17 | 山东师范大学 | Magnetic enrichment material, water body bacterium detection kit and application |
| CN111921511A (en) * | 2020-07-03 | 2020-11-13 | 西安交通大学 | Hydrogel-based cell stationary phase preparation method |
| CN111921511B (en) * | 2020-07-03 | 2023-01-03 | 西安交通大学 | Hydrogel-based cell stationary phase preparation method |
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Application publication date: 20170922 |