CN107185036A - A kind of micro nanometer fiber 3 D chitosan support and preparation method thereof - Google Patents
A kind of micro nanometer fiber 3 D chitosan support and preparation method thereof Download PDFInfo
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- CN107185036A CN107185036A CN201710434323.5A CN201710434323A CN107185036A CN 107185036 A CN107185036 A CN 107185036A CN 201710434323 A CN201710434323 A CN 201710434323A CN 107185036 A CN107185036 A CN 107185036A
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- chitosan
- nanometer fiber
- micro nanometer
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- support
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- 229920001661 Chitosan Polymers 0.000 title claims abstract description 37
- 239000000835 fiber Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 27
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000007710 freezing Methods 0.000 claims abstract description 4
- 230000008014 freezing Effects 0.000 claims abstract description 4
- 239000012046 mixed solvent Substances 0.000 claims abstract description 4
- 238000004108 freeze drying Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 6
- 239000002904 solvent Substances 0.000 abstract description 5
- 210000001519 tissue Anatomy 0.000 description 4
- 238000013019 agitation Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010041 electrostatic spinning Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005191 phase separation Methods 0.000 description 2
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 description 1
- 229920001287 Chondroitin sulfate Polymers 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000003519 biomedical and dental material Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 229940059329 chondroitin sulfate Drugs 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000003658 microfiber Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002121 nanofiber Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dispersion Chemistry (AREA)
- Materials For Medical Uses (AREA)
Abstract
The present invention relates to a kind of micro nanometer fiber 3 D chitosan support and preparation method thereof, it can obtain micro nanometer fiber 3 D chitosan support using the ternary mixed solvent dissolving chitosan of a certain proportion of acetic acid, tetrahydrofuran and water through a point sample, freeze forming and after freezing.The microstructure that micro nanometer fiber coexists assigns support good biocompatibility and mechanical strength.The preparation method technique is simple, compared with chitosan stent prepared by traditional binary solvent, and it is typically characterised by the microcosmic upper form that micro nanometer fiber is presented.
Description
Technical field
The present invention relates to a kind of micro nanometer fiber 3 D chitosan support and preparation method thereof, belong to bio-medical material skill
Art field.
Background technology
Chitosan be by a kind of natural alkaline polysaccharide, it is similar to the structure of chondroitin sulfate in human body, and amino brings
Alkalescence the features such as make it have anti-inflammation.Tissue engineering bracket prepared by chitosan has good mechanical property, is beneficial to
The Proliferation, Differentiation of cell, therefore have in tissue engineering material field important application.Conventional chitosan solvent is the water of acetic acid
Solution, the microstructure of the chitosan stent thus prepared is tens of sheet sponges to hundreds of micron thickness, with preferable
Mechanical strength and biology performance（Biomaterials,1999;20:1133-1142）.Hu Qiaoling et al. discloses three-dimensional order
The preparation method of porous chitosan scaffold material（CN101366972）, the reparation available for bone tissue.The shortcoming of chitosan stent
It is that the microstructure of its sheet is unfavorable for the adhesion and infiltration of cell, this is very not for the tissue engineering bracket of degradability
Profit.Research shows that nanometer fibrous support has higher specific surface area, beneficial to cell adherence and differentiation.But simple nanometer
Fiber mechanicses intensity is relatively low, it is impossible to bear the mechanical loading of stent implantation procedure.Comparatively speaking, micrometer fibers support has preferable
Mechanical property.Therefore, simultaneously there is nanometer and the microstructure of micrometer fibers can then overcome biocompatibility in the bracket
Contradiction between mechanical property.Micro nanometer fiber is prepared using method of electrostatic spinning more than current researcher, its advantage is that fiber is straight
Footpath can be controlled in certain limit, but method of electrostatic spinning can only obtain the film of two dimension, and preparation time is long.Traditional phase separation method
It is usually sheet honeycomb to prepare chitosan three-dimensional tissue engineering bracket, and its yardstick is generally at tens microns.Due to chitosan
Molecule has stronger molecular separating force, and it is dissolved in, and viscosity after acid is larger, and obtaining micro/nano fiber structure need to be by the dense of chitosan
Degree becomes very low, but the support being prepared is without mechanical strength, it is impossible to be used as tissue engineering bracket.
The content of the invention
The main imagination of the present invention is the binary solvent body for changing traditional water and acetic acid composition in phase separation preparation method
System, adds a kind of organic solvent and constitutes a kind of new ternary solvent system with water and acetic acid, change the molecular separating force of chitosan,
Chitosan is promoted to form micro nanometer fiber during freezing, so as to obtain micro nanometer fiber 3 D chitosan support.
The preparation method of micro nanometer fiber 3 D chitosan support is described in detail below in the present invention.
(1) ternary mixed solvent of acetic acid, tetrahydrofuran and water is prepared.
(2) chitosan is added, electromagnetic agitation dissolves 24 hours.
(3) after solution point sample, it is placed in cryogenic freezing shaping.
(4) freeze dryer is lyophilized 48 hours, can obtain micro nanometer fiber 3 D chitosan support.
Sample produced by the present invention is white foam, its 3D shape can by point sample using different containers and
Divide the regulation of sample volume.The microstructure that micro nanometer fiber coexists assigns support good biocompatibility and mechanical strength.The system
Preparation Method technique is simple, and with low cost, rack forming is good.Compared with chitosan stent prepared by traditional binary solvent, its
It is typically characterised by the microcosmic upper form that micro nanometer fiber is presented.
Embodiment
With reference to specific embodiment, present invention is further described, but the present invention implementation not
It is confined to this.
Embodiment 1：Acetic acid, tetrahydrofuran and water each 1ml, 5ml and 94ml are taken respectively, are well mixed.Weighing molecular weight is
100000 chitosan 0.5g, electromagnetic agitation dissolves 24 hours.After chitosan is completely dissolved, by chitosan solution point sample to container
In, freeze more than 12 hours and be molded at -80 DEG C, the three-dimensional shell of micro nanometer fiber can be obtained after being freezed 48 hours in freeze dryer and is gathered
Sugared support.
Embodiment 2：Acetic acid, tetrahydrofuran and water each 10ml, 20ml and 70ml are taken respectively, are well mixed.Weigh molecular weight
For 400,000 chitosan 3g, electromagnetic agitation dissolves 24 hours.After chitosan is completely dissolved, by chitosan solution point sample to container
In, at -196 DEG C（In liquid nitrogen）Lower freeze forming, can obtain the three-dimensional shell of micro nanometer fiber and gather after being freezed 48 hours in freeze dryer
Sugared support.
Claims (4)
1. a kind of micro nanometer fiber 3 D chitosan support and preparation method thereof, it is characterised in that dissolve the chitosan in acetic acid,
In the ternary mixed solvent of tetrahydrofuran and water, freeze forming at a certain temperature after point sample, freeze-drying is in freeze dryer
Micro nanometer fiber 3 D chitosan support can be obtained.
2. micro nanometer fiber 3 D chitosan support according to claim 1 and preparation method thereof, it is characterised in that made
Chitosan molecule amount is between 10~400,000, and the mass-volume concentration of chitosan is between 0.5~3%.
3. micro nanometer fiber 3 D chitosan support according to claim 1 and preparation method thereof, it is characterised in that ternary
The volume ratio of in the mixed solvent acetic acid is between 1~10%, and the volume ratio of tetrahydrofuran is between 5~20%, and the volume ratio of water exists
Between 70~94%.
4. micro nanometer fiber 3 D chitosan support according to claim 1 and preparation method thereof, it is characterised in that freezing
Forming temperature is between -80~-196 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710434323.5A CN107185036B (en) | 2017-06-09 | 2017-06-09 | Micro-nanofiber three-dimensional chitosan scaffold and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710434323.5A CN107185036B (en) | 2017-06-09 | 2017-06-09 | Micro-nanofiber three-dimensional chitosan scaffold and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107185036A true CN107185036A (en) | 2017-09-22 |
| CN107185036B CN107185036B (en) | 2020-03-31 |
Family
ID=59876246
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710434323.5A Expired - Fee Related CN107185036B (en) | 2017-06-09 | 2017-06-09 | Micro-nanofiber three-dimensional chitosan scaffold and preparation method thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN107185036B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747453A (en) * | 2012-07-05 | 2012-10-24 | 四川大学 | Porous superfine polymer fiber and preparation method thereof |
| CN103285424A (en) * | 2013-05-27 | 2013-09-11 | 东华大学 | Three-dimensional fiber-based aerogel tissue engineering scaffold and preparation method thereof |
| CN105641744A (en) * | 2016-02-27 | 2016-06-08 | 青岛大学 | Nano-micro multi-scale chitosan and polylactic acid composite scaffold and preparation method thereof |
| WO2017075554A1 (en) * | 2015-10-29 | 2017-05-04 | Golfetto Michael | Methods freeze drying and composite materials |
-
2017
- 2017-06-09 CN CN201710434323.5A patent/CN107185036B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747453A (en) * | 2012-07-05 | 2012-10-24 | 四川大学 | Porous superfine polymer fiber and preparation method thereof |
| CN103285424A (en) * | 2013-05-27 | 2013-09-11 | 东华大学 | Three-dimensional fiber-based aerogel tissue engineering scaffold and preparation method thereof |
| WO2017075554A1 (en) * | 2015-10-29 | 2017-05-04 | Golfetto Michael | Methods freeze drying and composite materials |
| CN105641744A (en) * | 2016-02-27 | 2016-06-08 | 青岛大学 | Nano-micro multi-scale chitosan and polylactic acid composite scaffold and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 刘强，孟范平，姚瑞华，张爱静: "添加剂聚乙二醇对壳聚糖超滤膜结构和性能的影响", 《膜科学与技术》 * |
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| Publication number | Publication date |
|---|---|
| CN107185036B (en) | 2020-03-31 |
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