CN107183507A - A kind of method that reference state heterocycle amine content is reduced based on acid amides active component - Google Patents
A kind of method that reference state heterocycle amine content is reduced based on acid amides active component Download PDFInfo
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Abstract
The invention discloses a kind of method that reference state heterocycle amine content is reduced based on acid amides active component, including, the solution of amide containing active component is uniformly added into beef gruel, baking box baking is put into after shaping.The method that acid amides active component provided by the present invention reduces reference state heterocycle amine content, can effectively be greatly reduced the content of reference state heterocyclic amine in roast beef, eat barbecue meat products safer, and method cost is low, is applicable to large-scale industrialized production.The method that acid amides active component provided by the present invention reduces reference state heterocycle amine content, reduces reference state heterocycle amine content, moreover it is possible to ensure that the local flavor more giving off a strong fragrance of barbecue meat products is lasting.The method that acid amides active component provided by the present invention reduces reference state heterocycle amine content, complies fully with natural, green, healthy development trend.
Description
Technical field
The invention belongs to food processing technology field, and in particular to one kind is miscellaneous based on acid amides active component reduction reference state
The method of cyclammonium content.
Background technology
Heterocyclic amine (Heterocyclic Amines, HAs) is to be rich in animal derived protein raw material in high-temperature cooking meat, fish etc.
During, because protein, amino acid, kreatinin and creatine are thermally decomposed in tissue, the class being made up of carbon, nitrogen, hydrogen has
There is carcinogenic, mutagenesis polycyclc aromatic compound.Research shows that long-term meat products of the intake containing HAs is remarkably improved mankind's trouble
Cancer risk.Therefore, HAs research turns into one of focus of attention of food security circle.So far, from high temperature meat product
Planted more than identification, the HAs30 for separating and there is carcinogenic mutagenicity using Ames/Salmonella experimental verifications.According to its structure
With constructive ways, two major classes can be broadly divided into：Aminooimidazole azepine aromatic hydrocarbons (aminoimidazoazarenes, AIAs) and ammonia
Base carboline class (carbolines).AIAs mainly includes：Quinolines, pyridines and quinoxaline, by glucose, free amine group
Acid, creatine and kreatinin are generated below 300 DEG C through thermal response, and the amino contained in its structure on imidazole ring, α positions can be in body
Inside it is converted into carcinogenic, mutagenicity N- hydroxy compounds.AIAs is alternatively referred to as IQ types heterocyclic amine or polar heterocyclic simultaneously
Amine.Amino carboline class heterocyclic amine mainly includes：α-carboline, B-carboline, gamma-carbolines and δ-carboline, are existed by protein or amino acid
It is pyrolyzed and produces during higher than 300 DEG C, its carcinogenic, Mutagenicity is weaker compared with IQ type heterocyclic amines, alternatively referred to as non-IQ types heterocycle
Amine or nonpolar heterocyclic amine.Wherein A α C, two kinds of α of MeA α C-carboline class heterocyclic amine are defined by international cancer research institution (IARC)
For potential carcinogen.
Reference state heterocyclic amine refers to that heterocyclic amine is combined the adduct to be formed with protein.China is meat products production, added
Work has Beef Pie, kebob, sausage etc. with consumption big country, major product.Heterocyclic amine material is prevalent in daily city
Sell in barbecue product, wherein reference state heterocyclic amine total amount can reach microgram rank.
Therefore, effectively control and reduce extensive pass of the method by academia of reference state heterocycle amine content in meat products
One of note, and the focus of attention as field of food safety.
The content of the invention
The purpose of this part is some aspects for summarizing embodiments of the invention and briefly introduces some preferably implementations
Example.It may do a little simplified or be omitted to avoid making our department in this part and the description of the present application summary and denomination of invention
Point, the purpose of specification digest and denomination of invention obscure, and this simplification or omit and cannot be used for limiting the scope of the present invention.
In view of there is the problem of reference state heterocycle amine content is high in above-mentioned and/or existing barbecue meat products, it is proposed that the present invention.
Therefore, the side of reference state heterocycle amine content is reduced based on acid amides active component it is an object of the present invention to provide a kind of
Method.
In order to solve the above technical problems, the invention provides following technical scheme：One kind is based on acid amides active component drop
The method of low combination state heterocycle amine content, including, the solution of amide containing active component is uniformly added into beef gruel, after shaping
It is put into baking box baking.
As a kind of method that reference state heterocycle amine content is reduced based on acid amides active component of the present invention, wherein：
The addition of the acid amides active component accounts for the 0.005~0.015% of the gross mass of beef gruel.
As a kind of method that reference state heterocycle amine content is reduced based on acid amides active component of the present invention, wherein：
The acid amides active component includes the one or more of capsaicine, numb-taste component of zanthoxylum or pipering.
As a kind of method that reference state heterocycle amine content is reduced based on acid amides active component of the present invention, wherein：
The acid amides active component includes capsaicine, numb-taste component of zanthoxylum, pipering, wherein, the quality of the capsaicine accounts for amide-type work
The 5~25% of property composition gross mass.
As a kind of method that reference state heterocycle amine content is reduced based on acid amides active component of the present invention, wherein：
The acid amides active component includes capsaicine, numb-taste component of zanthoxylum, pipering, wherein, the quality of the numb-taste component of zanthoxylum accounts for amide-type
The 35~55% of active component gross mass.
As a kind of method that reference state heterocycle amine content is reduced based on acid amides active component of the present invention, wherein：
The acid amides active component includes capsaicine, numb-taste component of zanthoxylum, pipering, wherein, the quality of the pipering accounts for amide-type work
The 15~55% of property composition gross mass.
As a kind of method that reference state heterocycle amine content is reduced based on acid amides active component of the present invention, wherein：
Described to be put into baking box baking, its baking temperature is 200~230 DEG C, and humidity is 80~95%, and cooking time is 15~25min.
As a kind of method that reference state heterocycle amine content is reduced based on acid amides active component of the present invention, wherein：
The beef gruel, its creatinine level is 0.70~0.95mg/g.
As a kind of method that reference state heterocycle amine content is reduced based on acid amides active component of the present invention, wherein：
The acid amides active component derives from natural flavor.
As a kind of method that reference state heterocycle amine content is reduced based on acid amides active component of the present invention, wherein：
The shaping, it is that beef is made into minced beef cutlet, and its thickness is 0.5~1.5cm, a diameter of 5~7cm.
The present invention is had the advantage that：
(1) method that acid amides active component provided by the present invention reduces reference state heterocycle amine content, can effectively significantly
The content of reference state heterocyclic amine in roast beef is reduced, eats barbecue meat products safer, and method cost is low, is applicable to big rule
Mould factorial praluction.
(2) method that acid amides active component provided by the present invention reduces reference state heterocycle amine content, reduces reference state
Heterocycle amine content, moreover it is possible to ensure that the local flavor more giving off a strong fragrance of barbecue meat products is lasting.
(3) method that acid amides active component provided by the present invention reduces reference state heterocycle amine content, complies fully with day
So, green, the development trend of health.
Embodiment
In order to facilitate the understanding of the purposes, features and advantages of the present invention, with reference to specific embodiment pair
The embodiment of the present invention is described in detail.
Many details are elaborated in the following description to facilitate a thorough understanding of the present invention, still the present invention can be with
It is different from other manner described here using other to implement, those skilled in the art can be without prejudice to intension of the present invention
In the case of do similar popularization, therefore the present invention is not limited by following public specific embodiment.
Secondly, " one embodiment " or " embodiment " referred to herein refers to may be included at least one realization side of the invention
Special characteristic, structure or characteristic in formula." in one embodiment " that different places occur in this manual not refers both to
Same embodiment, nor the single or selective embodiment mutually exclusive with other embodiment.
Embodiment 1
It will be completely dissolved from capsaicine, numb-taste component of zanthoxylum and pipering with ethanol in proper amount.
Beef gruel is made with meat grinder in fresh beef, creatinine level 0.85mg/ in beef gruel is measured with picric acid method
g。
Capsaicine, numb-taste component of zanthoxylum and pipering will be completely dissolved to be added in the beef gruel being made, mixed with cooking machine
Close uniform, rotating speed is 21000r/min, and the time is 2min.Diameter 6cm, thickness 1cm are made into 6cm culture dish, is weighed about
The total addition level of 40g meat pie, wherein capsaicine, numb-taste component of zanthoxylum and pipering accounts for the 0.005% of the minced beef cutlet weight.Capsicum
Plain adding proportion is 10%, and numb-taste component of zanthoxylum adding proportion is 35%, and pipering adding proportion is 55%
Meat pie is placed in baking box, the baking 20min under conditions of 225 DEG C, humidity is 90%, per each 10min of one side.
The minced beef cutlet of baking is crushed with pulverizer respectively, 4g samples is accurately weighed and 30mL 1mol/L sodium hydroxides is molten
Liquid is mixed, and 14g diatomite is added after homogeneous 1min and is stirred.50mL ethyl acetate is added in mixture and carries out 30min's
Ultrasonic extraction, same extraction is carried out twice, and remaining mixture carries out suction filtration, and smoke filtrate is with being that free state is miscellaneous in supernatant
Cyclammonium.Remaining mixture, which is volatilized, to be loaded after organic solvent in hydrolysis bottle, 40mL 6mol/L hydrochloric acid is added, in hydrolysis at 110 DEG C
24h, obtains the hydrolyzate of heterocyclic amine, and heterocyclic amine contained by this part is reference state heterocyclic amine.10mL hydrolyzates are taken to be loaded in advance
The Waters Oasis MCX solid phase extraction columns activated respectively with 6mL methanol, water, 0.1mol/L hydrochloric acid and ethyl acetate, then
Respectively pillar is rinsed with 6mL 0.1mol/L hydrochloric acid and water.After the drying of pillar vacuum, with 6mL ammoniated methanols (methanol aqueous ammonia volume
Than for 19:1) elute.The eluent finally given is dried up using nitrogen, and is redissolved with 300 μ L methanol.Redissolve liquid with 12000g from
After heart 10min, the UPLC-TQD ultra performance liquid chromatography QQ-TOF mass spectrometries that supernatant uses Waters, US are collected
Combined instrument is measured to heterocyclic amine contained therein.
Wherein, the condition of ultra performance liquid chromatography QQ-TOF mass spectrometry is respectively：Chromatographic condition：Chromatographic column：ACQUITYBEH C18 posts (2.1mm × 50mm, 1.7 μm)；Column temperature：35℃；Mobile phase：A phases are 10mmol/L ammonium acetates, and B phases are
100% acetonitrile；Gradient：0~0.1min be 90%A, 0.1~18min be 10%~30%B, 18~20min be 30%~
100%B, 20~20.1min are 100%~10%B；Flow velocity：0.3mL/min；Sample room temperature：4℃；Column temperature：35℃；Sample introduction
Amount：1μL.
Mass Spectrometry Conditions：Select multiple-reaction monitoring technology (Multi Reaction Monitoring, MRM)；Ionization side
Formula：Electron spray (ESI) positive ion mode second order mses；Ion source temperature：120℃；Desolvation temperature：350℃；Capillary electricity
Pressure：3.5kV；Taper hole throughput (nitrogen)：50L/h；Desolventizing gas flow (nitrogen)：800L/h；Collision gas flow (argon gas)：
0.13mL/min；Scanning range：2~2000Da.
Embodiment 2
It will be completely dissolved from capsaicine, numb-taste component of zanthoxylum and pipering with ethanol in proper amount.
Beef gruel is made with meat grinder in fresh beef, creatinine level 0.75mg/ in beef gruel is measured with picric acid method
g。
Capsaicine, numb-taste component of zanthoxylum and pipering will be completely dissolved to be added in the beef gruel being made, mixed with cooking machine
Close uniform, rotating speed is 21000r/min, and the time is 2min.Diameter 6cm, thickness 1cm are made into 6cm culture dish, is weighed about
The total addition level of 40g meat pie, wherein capsaicine, numb-taste component of zanthoxylum and pipering accounts for the 0.010% of the minced beef cutlet weight.Capsicum
Plain adding proportion is 20%, and numb-taste component of zanthoxylum adding proportion is 50%, and pipering adding proportion is 30%.
Meat pie is placed in baking box, the baking 20min under conditions of 225 DEG C, humidity is 90%, per each 10min of one side.
The minced beef cutlet of baking is crushed with pulverizer respectively, 4g samples is accurately weighed and 30mL 1mol/L sodium hydroxides is molten
Liquid is mixed, and 14g diatomite is added after homogeneous 1min and is stirred.50mL ethyl acetate is added in mixture and carries out 30min's
Ultrasonic extraction, same extraction is carried out twice, and remaining mixture carries out suction filtration, and smoke filtrate is with being that free state is miscellaneous in supernatant
Cyclammonium.Remaining mixture, which is volatilized, to be loaded after organic solvent in hydrolysis bottle, 40mL 6mol/L hydrochloric acid is added, in hydrolysis at 110 DEG C
24h, obtains the hydrolyzate of heterocyclic amine, and heterocyclic amine contained by this part is reference state heterocyclic amine.10mL hydrolyzates are taken to be loaded in advance
The Waters Oasis MCX solid phase extraction columns activated respectively with 6mL methanol, water, 0.1mol/L hydrochloric acid and ethyl acetate, then
Respectively pillar is rinsed with 6mL 0.1mol/L hydrochloric acid and water.After the drying of pillar vacuum, with 6mL ammoniated methanols (methanol aqueous ammonia volume
Than for 19:1) elute.The eluent finally given is dried up using nitrogen, and is redissolved with 300 μ L methanol.Redissolve liquid with 12000g from
After heart 10min, the UPLC-TQD ultra performance liquid chromatography QQ-TOF mass spectrometries that supernatant uses Waters, US are collected
Combined instrument is measured to heterocyclic amine contained therein.
Wherein, the condition of ultra performance liquid chromatography QQ-TOF mass spectrometry is respectively：Chromatographic condition：Chromatographic column：ACQUITYBEH C18 posts (2.1mm × 50mm, 1.7 μm)；Column temperature：35℃；Mobile phase：A phases are 10mmol/L ammonium acetates, and B phases are
100% acetonitrile；Gradient：0~0.1min be 90%A, 0.1~18min be 10%~30%B, 18~20min be 30%~
100%B, 20~20.1min are 100%~10%B；Flow velocity：0.3mL/min；Sample room temperature：4℃；Column temperature：35℃；Sample introduction
Amount：1μL.
Mass Spectrometry Conditions：Select multiple-reaction monitoring technology (Multi Reaction Monitoring, MRM)；Ionization side
Formula：Electron spray (ESI) positive ion mode second order mses；Ion source temperature：120℃；Desolvation temperature：350℃；Capillary electricity
Pressure：3.5kV；Taper hole throughput (nitrogen)：50L/h；Desolventizing gas flow (nitrogen)：800L/h；Collision gas flow (argon gas)：
0.13mL/min；Scanning range：2~2000Da.
Embodiment 3
It will be completely dissolved from capsaicine, numb-taste component of zanthoxylum and pipering with ethanol in proper amount.
Beef gruel is made with meat grinder in fresh beef, creatinine level 0.85mg/ in beef gruel is measured with picric acid method
g。
Capsaicine, numb-taste component of zanthoxylum and pipering will be completely dissolved to be added in the beef gruel being made, mixed with cooking machine
Close uniform, rotating speed is 21000r/min, and the time is 2min.Diameter 6cm, thickness 1cm are made into 6cm culture dish, is weighed about
The total addition level of 40g meat pie, wherein capsaicine, numb-taste component of zanthoxylum and pipering accounts for the 0.015% of the minced beef cutlet weight.Capsicum
Plain adding proportion is 25%, and numb-taste component of zanthoxylum adding proportion is 55%, and pipering adding proportion is 20%.
Meat pie is placed in baking box, the baking 20min under conditions of 225 DEG C, humidity is 90%, per each 10min of one side.
The minced beef cutlet of baking is crushed with pulverizer respectively, 4g samples is accurately weighed and 30mL 1mol/L sodium hydroxides is molten
Liquid is mixed, and 14g diatomite is added after homogeneous 1min and is stirred.50mL ethyl acetate is added in mixture and carries out 30min's
Ultrasonic extraction, same extraction is carried out twice, and remaining mixture carries out suction filtration, and smoke filtrate is with being that free state is miscellaneous in supernatant
Cyclammonium.Remaining mixture, which is volatilized, to be loaded after organic solvent in hydrolysis bottle, 40mL 6mol/L hydrochloric acid is added, in hydrolysis at 110 DEG C
24h, obtains the hydrolyzate of heterocyclic amine, and heterocyclic amine contained by this part is reference state heterocyclic amine.10mL hydrolyzates are taken to be loaded in advance
The Waters Oasis MCX solid phase extraction columns activated respectively with 6mL methanol, water, 0.1mol/L hydrochloric acid and ethyl acetate, then
Respectively pillar is rinsed with 6mL 0.1mol/L hydrochloric acid and water.After the drying of pillar vacuum, with 6mL ammoniated methanols (methanol aqueous ammonia volume
Than for 19:1) elute.The eluent finally given is dried up using nitrogen, and is redissolved with 300 μ L methanol.Redissolve liquid with 12000g from
After heart 10min, the UPLC-TQD ultra performance liquid chromatography QQ-TOF mass spectrometries that supernatant uses Waters, US are collected
Combined instrument is measured to heterocyclic amine contained therein.
Wherein, the condition of ultra performance liquid chromatography QQ-TOF mass spectrometry is respectively：Chromatographic condition：Chromatographic column：ACQUITYBEH C18 posts (2.1mm × 50mm, 1.7 μm)；Column temperature：35℃；Mobile phase：A phases are 10mmol/L ammonium acetates, and B phases are
100% acetonitrile；Gradient：0~0.1min be 90%A, 0.1~18min be 10%~30%B, 18~20min be 30%~
100%B, 20~20.1min are 100%~10%B；Flow velocity：0.3mL/min；Sample room temperature：4℃；Column temperature：35℃；Sample introduction
Amount：1μL.
Mass Spectrometry Conditions：Select multiple-reaction monitoring technology (Multi Reaction Monitoring, MRM)；Ionization side
Formula：Electron spray (ESI) positive ion mode second order mses；Ion source temperature：120℃；Desolvation temperature：350℃；Capillary electricity
Pressure：3.5kV；Taper hole throughput (nitrogen)：50L/h；Desolventizing gas flow (nitrogen)：800L/h；Collision gas flow (argon gas)：
0.13mL/min；Scanning range：2~2000Da.
Embodiment 4
By the measurement result analysis and arrangement of above-described embodiment 1~3, concrete outcome see the table below.
Amide-type activity in the mixing natural flavor described in blank minced beef cutlet and addition three kinds of embodiments of the invention in table
After composition in minced beef cutlet reference state heterocyclic amine content (ng/g).
| MeIQ | 4,8-DiMeIQx | Total | |
| Blank minced beef cutlet | 6.73±0.23 | 0.86±0.10 | 1679.51±79.15 |
| Embodiment 1 | 2.88 ± 0.28 (57%) | 0.32 ± 0.02 (63%) | 502.38 ± 11.99 (70%) |
| Embodiment 2 | 4.38 ± 0.12 (35%) | 0.41 ± 0.03 (52%) | 427.34 ± 11.23 (75%) |
| Embodiment 3 | 2.56 ± 0.19 (62%) | Nd (100%) | 561.75 ± 11.04 (67%) |
Note：The heterocycle amine content of each condition is three repetitions, is as a result represented with mean value ± standard deviation；It is phase in bracket
The inhibiting rate to specific heterocyclic amine after acid amides active component is added in present invention mixing natural flavor to blank minced beef cutlet.
As can be seen from the table, the acid amides active component quinoline stronger to carcinogenicity in natural flavor is mixed in the present invention
Quinoline class heterocyclic amine MeIQ and quinoxaline heterocyclic amine 4,8-DiMeIQx and reference state heterocyclic amine total amount have significant suppression effect
Really.
Embodiment 5
After minced beef cutlet pretreatment made from embodiment 1~3, flavor substance measure is carried out, is specially：Take 8mL reactions anti-
Answer liquid in 15mL ml headspace bottle, cover immediately, in 50 DEG C of water-baths after constant temperature 35min, insert extracting head, probe is placed in sample
Top, the probe extracted takes out, is directly pyrolyzed on GC-MS combined instruments immediately by extraction time 40min from ml headspace bottle
Inhale, 250 DEG C of thermal desorption temperature, desorption time 3min, while starting instrument gathered data.
GC conditions：DB-WAX capillary columns (30m × O.25mm, 0.25 μm)；250 DEG C of injector temperature；Carrier gas is nitrogen；
Flow velocity 1.2mL/min；Split ratio is 10：1；Heating schedule：40 DEG C of holding 2min, then rise to 200 DEG C, then with 3 DEG C/min
250 DEG C are risen to 5 DEG C/min, 5min is kept.
Ms conditions：Electron ionization sources；Electron energy 70eV：The μ A of filament emission current 20；200 DEG C of ion source temperature；Interface
250 DEG C of temperature；Full scan；Mass scan range m/z 33~495.
Flavor substance measurement result shows, compared to blank minced beef cutlet, the roast beef main flavor thing in embodiment 1~3
The amount of matter pyrazine has different degrees of lifting, and this has great role to the flavor quality for lifting minced beef cutlet.
As can be seen here, the method that acid amides active component provided by the present invention reduces reference state heterocycle amine content, can have
The content of reference state heterocyclic amine in roast beef is greatly reduced in effect, eats barbecue meat products safer, and method cost is low, applicable
In large-scale industrialized production；The method that acid amides active component provided by the present invention reduces reference state heterocycle amine content, drop
Low combination state heterocycle amine content, moreover it is possible to ensure that the local flavor more giving off a strong fragrance of barbecue meat products is lasting；Amide-type provided by the present invention is lived
The method of property constituent reduction reference state heterocycle amine content, complies fully with natural, green, healthy development trend.
It is noted that present invention applicant passes through lot of experiments and creative work, the flesh in beef is analyzed
Acid anhydride acid content within the specific limits, can reduce the generation of the reference state heterocyclic amine in post-production.And in testing also
It was found that, effect is used alone typically in three kinds of natural acid amides active components involved in the present invention, and for example capsaicine is to PhIP's
Inhibition very well, and is ineffective to other several heterocyclic amines.Therefore the inhibition of the protrusion of the invention reached,
Realized under a kind of synergy, i.e., the content of creatinine, the proportioning of three kinds of natural acid amides active components and they
With the collective effect of the proportioning and baking condition of Quality Beef these optimum conditions.Its application point is that we invents offer
Technical scheme can suppress flesh liver and pass through aminooimidazole part in cyclisation and dehydration formation molecule, suppress pyrazine and pyrrole
The participation Maillard reaction formation heterocyclic amine of pyridine radical cation or Ionization Potential of C-Centered Radicals.Flavor substance be Pyrazine herein
Obtain a certain degree of protection.Free state is generated at relatively high temperatures, and reference state can be generated at a lower temperature, therefore is ground
Studying carefully reference state heterocyclic amine has the bigger meaning of specific ionization state heterocyclic amine outstanding.
It should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to preferable
The present invention is described in detail embodiment, it will be understood by those within the art that, can be to technology of the invention
Scheme is modified or equivalent substitution, and without departing from the spirit and scope of technical solution of the present invention, it all should cover in this hair
Among bright right.
Claims (10)
1. a kind of method that reference state heterocycle amine content is reduced based on acid amides active component, it is characterised in that including：
The solution of amide containing active component is uniformly added into beef gruel, baking box baking is put into after shaping.
2. the method for reference state heterocycle amine content is reduced based on acid amides active component as claimed in claim 1, it is characterised in that：
The addition of the acid amides active component accounts for the 0.005~0.015% of the gross mass of beef gruel.
3. reducing the method for reference state heterocycle amine content based on acid amides active component as claimed in claim 1 or 2, its feature exists
In：The acid amides active component includes the one or more of capsaicine, numb-taste component of zanthoxylum or pipering.
4. the method for reference state heterocycle amine content is reduced based on acid amides active component as claimed in claim 3, it is characterised in that：
The acid amides active component includes capsaicine, numb-taste component of zanthoxylum, pipering, wherein, the quality of the capsaicine accounts for amide-type work
The 5~25% of property composition gross mass.
5. the method for reference state heterocycle amine content is reduced based on acid amides active component as claimed in claim 4, it is characterised in that：
The acid amides active component includes capsaicine, numb-taste component of zanthoxylum, pipering, wherein, the quality of the numb-taste component of zanthoxylum accounts for amide-type
The 35~55% of active component gross mass.
6. the method for reference state heterocycle amine content is reduced based on acid amides active component as claimed in claim 5, it is characterised in that：
The acid amides active component includes capsaicine, numb-taste component of zanthoxylum, pipering, wherein, the quality of the pipering accounts for amide-type work
The 15~55% of property composition gross mass.
7. reference state heterocycle amine content is reduced based on acid amides active component as any one of claim 1,2,4,5 or 6
Method, it is characterised in that：Described to be put into baking box baking, its baking temperature is 200~230 DEG C, and humidity is 80~95%, baking
Time is 15~25min.
8. the method for reference state heterocycle amine content is reduced based on acid amides active component as claimed in claim 7, it is characterised in that：
The beef gruel, its creatinine level is 0.70~0.95mg/g.
9. contained as any one of claim 1,2,4,5,6 or 8 based on acid amides active component reduction reference state heterocyclic amine
The method of amount, it is characterised in that：The acid amides active component derives from natural flavor.
10. reducing the method for reference state heterocycle amine content based on acid amides active component as claimed in claim 9, its feature exists
In：The shaping, it is that beef is made into minced beef cutlet, and its thickness is 0.5~1.5cm, a diameter of 5~7cm.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108709788A (en) * | 2018-04-23 | 2018-10-26 | 河南科高中标检测技术有限公司 | The methods for commenting method to measure gingerol content in ginger medicinal substances extract are surveyed using one more |
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| CN108709788A (en) * | 2018-04-23 | 2018-10-26 | 河南科高中标检测技术有限公司 | The methods for commenting method to measure gingerol content in ginger medicinal substances extract are surveyed using one more |
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| CN109077249B (en) * | 2018-08-31 | 2022-07-29 | 江南大学 | Method for reducing content of heterocyclic amine in sausage |
| CN112293679A (en) * | 2019-07-30 | 2021-02-02 | 昆明芳本生物科技发展有限公司 | Composition for inhibiting heterocyclic amine and preparation method and application thereof |
| CN111418786A (en) * | 2020-05-13 | 2020-07-17 | 吉林大学 | Preparation method of low-heterocyclic amine sauced beef |
| CN116268298A (en) * | 2023-03-27 | 2023-06-23 | 吉林大学 | Preparation method of low-heterocyclic amine mountain black pig barbecue |
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