CN107176969A - A kind of sucking pig liver small molecular extract and application thereof - Google Patents
A kind of sucking pig liver small molecular extract and application thereof Download PDFInfo
- Publication number
- CN107176969A CN107176969A CN201710137504.1A CN201710137504A CN107176969A CN 107176969 A CN107176969 A CN 107176969A CN 201710137504 A CN201710137504 A CN 201710137504A CN 107176969 A CN107176969 A CN 107176969A
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- sucking pig
- pig liver
- small molecular
- extract
- molecular extract
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K4/00—Peptides having up to 20 amino acids in an undefined or only partially defined sequence; Derivatives thereof
- C07K4/12—Peptides having up to 20 amino acids in an undefined or only partially defined sequence; Derivatives thereof from animals; from humans
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of sucking pig liver small molecular extract and application thereof；Aim to provide a kind of preparation method simple, the significant sucking pig liver small molecular extract of drug effect, the small molecular extract provides new medicine for chronic hepatitis B inactivity hepatitis B surface antigen carrier；Its technical scheme is such：Described sucking pig liver small molecular extract is to extract to be made from sucking pig liver.
Description
Technical field
The invention discloses a kind of sucking pig liver small molecular extract, the invention further relates to the sucking pig liver small molecular extract
Purposes.
Background technology
There are 2,000,000,000 population infection hepatitis Bs in the whole world, wherein 2.4 hundred million be chronic HBV surface antigen (HBsAg)
Carrier.Chronic hepatitis B (CHB) some people is sluggish, but some people due to HBsAg lasting masculins can
Cause hepatic sclerosis, liver cancer.
In recent years, using interferon and nucleoside analogue treatment CHB, remarkable progress is obtained, Most patients are by treatment
Afterwards, serum glutamic pyruvic transminase (ALT), HBVDNA, HBeAg are in peripheral blood can recover normal or switch to feminine gender, but peripheral blood
HBsAg still lasting masculins, are extremely difficult to serological conversion (Anti-HBs antibody being presented positive).In these years, domestic and foreign scholars have been proposed
Chronic hepatitis B feature cure concept, that is to say, that treatment chronic hepatitis B except require ALT, HBVDNA,
Beyond HBeAg is steady in a long-term normal, still require that peripheral blood HBsAg is eliminated, and occur hepatitis B surface antibody, i.e. serology by
The HBsAg positives are converted to feminine gender, and Anti-HBs antibody occur this is also terminal or target to treatment of chronic.Reach this
Target, is exactly that CHB features are cured.
The content of the invention
In view of the above-mentioned problems, simple it is an object of the invention to provide a kind of preparation method, the significant sucking pig liver of drug effect is small
Molecule extractive, and the sucking pig liver small molecular extract is provided in preparation treatment chronic hepatitis B inactivity hepatitis B surface antigen
The application of carrier's oral drugs, for chronic hepatitis B, inactivity hepatitis B surface antigen carrier provides new medicine.
In order to solve the above technical problems, the technical scheme that the present invention is provided is such：A kind of sucking pig liver small molecule is extracted
Thing, described sucking pig liver small molecular extract is that obtained micromolecule polypeptide class material is extracted from sucking pig liver.
The preparation method of above-mentioned sucking pig liver small molecular extract, passes sequentially through following step and is prepared：
Step 1：Take after the pretreatment of sucking pig Liver Channel first, homogenate obtains homogenate after being mixed with physiological saline；
Step 2：Carry out ultrafiltration to homogenate with 20KD ultrafilters, then with 6KD milipore filter nanofiltrations；
Step 3：Mutually enter the epicoele pushing piston of gc column tube using high-pressure flow to the mixing homogenate for entering cavity of resorption
Body is descending to implement compression, reaches that orderly, close-packed arrays turn into stationary phase and pressurize again；Separated material take high-pressure delivery from
Feed tube enters, by upper sieve plate and distributor, and filtering is separated the impurity in material, and after uniform divided flows in stationary phase quilt
Absorption, then enter from feed tube through high-pressure flow release stationary phase in adsorbed separated material, according to this from distributor and
Lower sieve plate, respective collection vessel is flowed into by drain pipe, obtains sucking pig liver small molecular extract after purification.
Further, the molecular weight of described sucking pig liver small molecular extract is below 10000 dalton.
A kind of purposes of sucking pig liver small molecular extract as described above, for preparing treatment chronic hepatitis B inactivity second
Liver HBsAg carrier oral drugs.
Compared with prior art, a kind of sucking pig liver small molecular extract that the present invention is provided, significantly, extracting method is simple for drug effect
It is single, and can be chronic as the application in treatment chronic hepatitis B inactivity surface carrier's oral drugs are prepared
The new medicine of liver inactivity hepatitis B surface antigen carrier offer and treatment method.
Embodiment
With reference to embodiment, the claim to the present invention is described in further detail, but is not constituted pair
Any limitation of the present invention, the modification of any limited number of time made in the claims in the present invention protection domain, still in the present invention
Claims within.
Embodiment 1
The sucking pig liver small molecular extract that the present invention is provided is to be walked from sucking pig liver by homogenate, ultrafiltration, liquid chromatography purification
The rapid small molecular extract for obtaining higher concentration, with the bioactivity for starting congenital inherent immunity reaction, produces similar Toll
Sample receptor activators are acted on, namely produce large amounts of type i and interferon type Ⅱ, so as to suppress or remove hepatitis B virus duplication.This is carried
Mouse hepatitis B model that thing sets up to hydrodynamic(al) force method (by oral, daily gavage once, continuous 10 weeks) is taken, is as a result shown to reality
The hepatitis b surface antigen positive for testing model mice switchs to feminine gender, and hepatitis B surface antibody occurs, illustrates that the present invention can be as chronic
The medicine for oral use of hepatitis B inactivity hepatitis B surface antigen carrier.
HBsAg dynamic changes are shown in Table 1 before and after treatment, and Anti-HBs antibody dynamic change is shown in Table 2 before and after treatment.
Table 1
※ is compared P ＜ 0.05 with saline control group.
Table 2
※ is compared P ＜ 0.05 with saline control group.
Above-described is only presently preferred embodiments of the present invention, all timess made in the range of the spirit and principles in the present invention
What modifications, equivalent substitutions and improvements etc., should be included in the scope of the protection.
Claims (3)
1. a kind of sucking pig liver small molecular extract, it is characterised in that described sucking pig liver small molecular extract is from sucking pig liver
Extract obtained micromolecule polypeptide class material.
2. sucking pig liver small molecular extract according to claim 1, it is characterised in that described sucking pig liver small molecule is extracted
The molecular weight of thing is below 10000 dalton.
3. a kind of purposes of sucking pig liver small molecular extract as claimed in claim 1, it is characterised in that slow for preparing treatment
Property hepatitis B inactivity hepatitis B surface antigen carrier's oral drugs.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610136878 | 2016-03-10 | ||
| CN2016101368787 | 2016-03-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107176969A true CN107176969A (en) | 2017-09-19 |
Family
ID=59829756
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710137504.1A Pending CN107176969A (en) | 2016-03-10 | 2017-03-09 | A kind of sucking pig liver small molecular extract and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107176969A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110302359A (en) * | 2018-08-13 | 2019-10-08 | 广东思峰生物科技有限责任公司 | A kind of preparation reducing blood glucose, preparation method and applications |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1041949A (en) * | 1989-09-13 | 1990-05-09 | 中国人民解放军空军广州医院 | Adopt the negative pressure dialysis method to prepare the method for hepatocyte growth factor |
| CN1085803A (en) * | 1992-10-20 | 1994-04-27 | 空军第四研究所 | Medicine for improving growth of liver cell and its method for production |
| CN104388509A (en) * | 2014-10-27 | 2015-03-04 | 合肥平光制药有限公司 | Preparation method of hepatocyte growth-promoting factors employing ultrasonic treatment |
| CN104388510A (en) * | 2014-10-27 | 2015-03-04 | 合肥平光制药有限公司 | High-extraction rate preparation method of high-activity hepatocyte growth-promoting factor |
-
2017
- 2017-03-09 CN CN201710137504.1A patent/CN107176969A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1041949A (en) * | 1989-09-13 | 1990-05-09 | 中国人民解放军空军广州医院 | Adopt the negative pressure dialysis method to prepare the method for hepatocyte growth factor |
| CN1085803A (en) * | 1992-10-20 | 1994-04-27 | 空军第四研究所 | Medicine for improving growth of liver cell and its method for production |
| CN104388509A (en) * | 2014-10-27 | 2015-03-04 | 合肥平光制药有限公司 | Preparation method of hepatocyte growth-promoting factors employing ultrasonic treatment |
| CN104388510A (en) * | 2014-10-27 | 2015-03-04 | 合肥平光制药有限公司 | High-extraction rate preparation method of high-activity hepatocyte growth-promoting factor |
Non-Patent Citations (3)
| Title |
|---|
| 何伟等: "促肝细胞生长素(HGF)治疗慢性乙型肝炎HBv抗原阴转效果观察", 《吉林医学》 * |
| 张琴等: "促肝细胞生长素肠溶胶囊治疗慢性乙型肝炎及肝硬化的临床观察", 《肝脏》 * |
| 熊益群: "《肝脏疾病用药专柜》", 30 September 2000, 中国协和医科大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110302359A (en) * | 2018-08-13 | 2019-10-08 | 广东思峰生物科技有限责任公司 | A kind of preparation reducing blood glucose, preparation method and applications |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170919 |