CN107176697A - Cicada fungus flocculant - Google Patents
Cicada fungus flocculant Download PDFInfo
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- CN107176697A CN107176697A CN201710529376.5A CN201710529376A CN107176697A CN 107176697 A CN107176697 A CN 107176697A CN 201710529376 A CN201710529376 A CN 201710529376A CN 107176697 A CN107176697 A CN 107176697A
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- Prior art keywords
- flocculant
- cicada fungus
- spore
- spore suspension
- mixed liquor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The invention discloses a kind of cicada fungus flocculant, it is desirable to provide the microbial flocculant that a kind of preservation is easy to carry, performance is stable.Its step is；The cicada fungus bacterial strain of Cord blood is inoculated with into the dose inoculation of three flat boards by every plant to be activated to PDA plate, constant temperature is inverted culture, the spore under sterile washing on flat board；Adjust the spore concentration in spore suspension；The spore suspension of the concentration is inoculated into the triangular flask of PDA liquid medium, isothermal vibration fermented and cultured；Centrifuge zymotic fluid；Concentrated supernatant, adds absolute ethyl alcohol, and vibration is mixed, and obtains mixed liquor；Mixed liquor is centrifuged, ethanol cleaning sediment is freeze-dried to obtain cicada fungus flocculant crude product.The present invention is for ease of the dry product for preserving and carrying, and optimal flocculating rate is up to 91.55 ± 2.43%；It is a kind of organism waste water inorganic agent.
Description
Technical field
The present invention relates to a kind of microbial flocculant, more particularly to a kind of cicada fungus flocculant；Belong to sewage treatment flocculating agent.
Background technology
Flocculant be a class can by the colloid or flocculation in suspension into the natural subsidence compared with big flocculating body thing
Matter, flocculation is exactly that flocculant makes suspension reach a process of separation of solid and liquid；Be widely used wastewater treatment, drinking water
The fields such as purification.Divided according to its structure and property, flocculant can be divided into inorganic flocculating agent, organic flocculant and microorganism
Flocculant.Current most of flocculants are all inorganic salts or organic polymer.Inorganic salts flocculant mainly has aluminium salt and molysite,
Although cost is relatively low, the aluminium ion remained in water can cause secondary pollution；Iron ion has color in itself, and has corruption to equipment
Erosion is acted on.In addition, inorganic salts flocculant dosage is big, sludge output is high, therefore increase operating cost.Synthesize flocculating polymer
Agent is although adding less, formation flco is big, flco effect is good, and non-corrosiveness, but a certain amount of remaining single due to existing
Body acrylamide, inevitably brings chemical toxicity, so its application is restricted.
Microbial flocculant belongs to natural macromolecule flocculating agent, relative to traditional flocculant have it is degradable, without secondary dirt
Dye, security are good, easily realize that separation of solid and liquid, precipitation are few, pH and heat endurance are good, and turbidity removal ability is strong, and consumption is few, uses model
Enclose and wait many advantages extensively；Therefore had a good application prospect in terms of water process, food processing and fermentation industry,
The focus researched and developed as domestic and international New Type Water Treatment Chemicals.
Cicada fungus is a kind of entomogenous fungi, the medicinal history with nearly one thousand years；It is a kind of safe fungus resource.Develop cicada
The flocculant of peanut production, extracts active material therein, and the microbial flocculant higher by a kind of security is obtained, is its industry
Change the core of application.
At present, be typically all that the zymotic fluid that produces bacterial strain directly applies to water process, exist use, carry it is inconvenient,
Not easy to maintain the shortcomings of.
The content of the invention
For drawbacks described above present in prior art, the present invention is intended to provide one kind is easy to preserve and carried, performance is steady
Fixed cicada fungus flocculant.
To achieve these goals, the present invention uses following technical scheme：
1) the cicada fungus bacterial strain of Cord blood is inoculated with into the dose inoculation of three flat boards by every plant to be lived to PDA plate
Change, 5~7d of culture is inverted in 25 DEG C of constant incubators, then with the spore under sterile washing on flat board, spore suspension is obtained；
2) spore concentration in adjustment spore suspension is up to 1 × 107Individual/mL；
3) it is 200mL/ by every plant of 3 parallel dose inoculations to bottling amount by the spore suspension adjusted through over-richness
In 500mL PDA liquid medium triangular flask, fermented and cultured 7d in isothermal vibration incubator is placed in；Concussion frequency is 140r/
Min, cultivation temperature are 25 DEG C；
4) zymotic fluid is placed in a centrifuge processing 100min, collects supernatant, centrifuge speed is 6000r/min；
5) supernatant is placed in 50 DEG C of Rotary Evaporators and is concentrated into the 1/3 of original volume, add 3~4 times of volumes,
Temperature is 4 DEG C of absolute ethyl alcohol, and vibration is mixed after standing 18~24h in 4 DEG C of environment, obtains mixed liquor；
6) mixed liquor is placed in a centrifuge processing 15min, sediment is cleaned twice with 95% ethanol, -46 DEG C
Under the conditions of freeze-drying 24h obtain cicada fungus flocculant crude product；Centrifuge speed is 6000r/min.
Compared with the prior art, the present invention is as a result of freeze drying process, and not only performance is steady for its flocculant prepared
Fixed, optimal flocculating rate is up to 91.55 ± 2.43%, and flocculant can effectively extend for ease of the dry product for preserving and carrying
The commodity price of flocculant.
Embodiment
With reference to specific embodiment, the invention will be further described, comprises the following steps that：
1) the cicada fungus bacterial strain of Cord blood is inoculated with into the dose inoculation of three flat boards by every plant to be lived to PDA plate
Change, 5~7d of culture is inverted in 25 DEG C of constant incubators, then with the spore under sterile washing on flat board, spore suspension is obtained；
2) spore concentration in adjustment spore suspension is up to 1 × 107Individual/mL；
3) it is 200mL/ by every plant of 3 parallel dose inoculations to bottling amount by the spore suspension adjusted through over-richness
In 500mL PDA liquid medium triangular flask, fermented and cultured 7d in isothermal vibration incubator is placed in；Concussion frequency is 140r/
Min, cultivation temperature are 25 DEG C；
4) zymotic fluid is placed in a centrifuge processing 100min, collects supernatant, centrifuge speed is 6000r/min；
5) supernatant is placed in 50 DEG C of Rotary Evaporators and is concentrated into the 1/3 of original volume, add 3~4 times of volumes,
Temperature is 4 DEG C of absolute ethyl alcohol, and vibration is statically placed in 18~24h in 4 DEG C of environment after mixing, obtains mixed liquor；
6) mixed liquor is placed in a centrifuge processing 15min, sediment is cleaned twice with 95% ethanol, -46 DEG C
Under the conditions of freeze-drying 24h obtain cicada fungus flocculant crude product；Centrifuge speed is 6000r/min.
The following is the simulated test made using present invention processing Kaolin clay suspension.
By flocculating agent formulation of the present invention into the liquid that concentration is 1g/L, by 0,0.4,0.8,1.2,1.6,2,2.4,2.8,
3.2nd, 3.6,4,4.4mL dosage is injected separately into 50mL colorimetric cylinder, and the aqueous suspension ofkaolin for being 4g/L with concentration is supplied
50mL；Colorimetric cylinder is reverse 10 times (rise to finish by bubble every time and be defined), stand 20min；Below liquid level 1cm liquid is taken to exist
Light absorption value is determined at 550nm, the light absorption value to add distilled water each handles 3 Duplicate Samples, determine what is isolated and purified as control
The flocculation activity of flocculant, is represented with flocculating rate E (%), obtains optimum dosage.
Flocculating rate calculation formula：Flocculating rate E (%)=(A-B)/A × 100%
In formula, A represents that the light absorption value, the B that add the kaolinite soil suspension that distilled water is measured represent to add the height that zymotic fluid is measured
The light absorption value of ridge soil suspension.Testing result is shown in Table 1.
Table 1：Extract the flocculation activity of the cicada fungus flocculant prepared
| Experiment numbers | Addition (mL/50mL) | Repeat number | Flocculating rate (%) |
| 1 | 0.4 | 3 | 60.92±4.74 |
| 2 | 0.8 | 3 | 82.42±5.70 |
| 3 | 1.2 | 3 | 91.55±2.43 |
| 4 | 1.6 | 3 | 89.76±1.85 |
| 5 | 2.0 | 3 | 86.49±2.04 |
| 6 | 2.4 | 3 | 84.76±2.33 |
| 7 | 2.8 | 3 | 81.04±2.43 |
| 8 | 3.2 | 3 | 78.54±1.20 |
| 9 | 3.6 | 3 | 74.88±3.85 |
| 10 | 4.0 | 3 | 73.97±3.92 |
| 11 | 4.4 | 3 | 72.78±3.89 |
As can be seen from Table 1：Influence of the different dosages to its flocculation efficiency is different.When flocculant dosage be 0.4~
During 1.2mL/50mL mixed liquors (flocculant liquid+aqueous suspension ofkaolin), flocculating rate increases with the increase of dosage, and
Peak 91.55 ± 2.43% is reached during 1.2mL；But when flocculant dosage is 1.2~4.4mL/50mL mixed liquors, flocculation
Rate is reduced with the increase of dosage.This is probably the adsorbed polymer of suspension bulky grain when flocculant addition increases
Surround, a kind of new mutually exclusive charge balance has been reached between suspension, so as to cause flocculating rate to decline.
Claims (1)
1. a kind of cicada fungus flocculant, including cicada fungus culture；It is characterized in that preparation method is as follows：
1) the cicada fungus bacterial strain of Cord blood is inoculated with into the dose inoculation of three flat boards by every plant to be activated to PDA plate, in
5~7d of culture is inverted in 25 DEG C of constant incubators, then with the spore under sterile washing on flat board, spore suspension is obtained；
2) spore concentration in adjustment spore suspension is up to 1 × 107Individual/mL；
3) it is 200mL/500mL by every plant of 3 parallel dose inoculations to bottling amount by the spore suspension adjusted through over-richness
PDA liquid medium triangular flask in, be placed in fermented and cultured 7d in isothermal vibration incubator；Concussion frequency is 140r/min, training
It is 25 DEG C to support temperature；
4) zymotic fluid is placed in a centrifuge processing 100min, collects supernatant, centrifuge speed is 6000r/min；
5) supernatant is placed in 50 DEG C of Rotary Evaporators and is concentrated into the 1/3 of original volume, add 3~4 times of volumes, temperature
For 4 DEG C of absolute ethyl alcohol, vibration is mixed after standing 18~24h in 4 DEG C of environment, obtains mixed liquor；
6) mixed liquor is placed in a centrifuge processing 15min, cleans sediment twice with 95% ethanol, -46 DEG C of conditions
Lower freeze-drying 24h obtains cicada fungus flocculant crude product；Centrifuge speed is 6000r/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710529376.5A CN107176697A (en) | 2017-07-01 | 2017-07-01 | Cicada fungus flocculant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710529376.5A CN107176697A (en) | 2017-07-01 | 2017-07-01 | Cicada fungus flocculant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107176697A true CN107176697A (en) | 2017-09-19 |
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ID=59845206
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710529376.5A Pending CN107176697A (en) | 2017-07-01 | 2017-07-01 | Cicada fungus flocculant |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101070203A (en) * | 2007-06-18 | 2007-11-14 | 同济大学 | Method for preparing biological flocculant using strange deformation bacilli |
| CN102250777A (en) * | 2011-06-20 | 2011-11-23 | 贵州大学 | Isariacicadae strain and use thereof |
| JP2014024044A (en) * | 2012-07-30 | 2014-02-06 | Tomooka Kaken Kk | Cation high polymer coagulant |
| CN103571756A (en) * | 2012-07-30 | 2014-02-12 | 浙江泛亚生物医药股份有限公司 | Test tube screening method of isaria cicadae strain and culture medium |
| US20140284273A1 (en) * | 2012-04-17 | 2014-09-25 | Environmental Dynamics International, Inc. | Decanted bio-balanced reactor and method |
| CN106698680A (en) * | 2017-02-14 | 2017-05-24 | 厦门大学 | Method for adsorbing heavy metal lead by using biological flocculating agent |
-
2017
- 2017-07-01 CN CN201710529376.5A patent/CN107176697A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101070203A (en) * | 2007-06-18 | 2007-11-14 | 同济大学 | Method for preparing biological flocculant using strange deformation bacilli |
| CN102250777A (en) * | 2011-06-20 | 2011-11-23 | 贵州大学 | Isariacicadae strain and use thereof |
| US20140284273A1 (en) * | 2012-04-17 | 2014-09-25 | Environmental Dynamics International, Inc. | Decanted bio-balanced reactor and method |
| JP2014024044A (en) * | 2012-07-30 | 2014-02-06 | Tomooka Kaken Kk | Cation high polymer coagulant |
| CN103571756A (en) * | 2012-07-30 | 2014-02-12 | 浙江泛亚生物医药股份有限公司 | Test tube screening method of isaria cicadae strain and culture medium |
| CN106698680A (en) * | 2017-02-14 | 2017-05-24 | 厦门大学 | Method for adsorbing heavy metal lead by using biological flocculating agent |
Non-Patent Citations (1)
| Title |
|---|
| 郭旭辉等: "一株蝉拟青霉菌产絮凝活性的研究", 《武汉大学学报(理学版)》 * |
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Application publication date: 20170919 |
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