CN107175089A - A kind of iron-nickel magnetic microballoon of cobalt ions that adulterates and its application - Google Patents

A kind of iron-nickel magnetic microballoon of cobalt ions that adulterates and its application Download PDF

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CN107175089A
CN107175089A CN201710417423.7A CN201710417423A CN107175089A CN 107175089 A CN107175089 A CN 107175089A CN 201710417423 A CN201710417423 A CN 201710417423A CN 107175089 A CN107175089 A CN 107175089A
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iron
nickel
cobalt ions
magnetic
cobalt
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刘宝全
李林
张艳梅
李春斌
权春善
范圣第
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Dalian Minzu University
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Dalian Nationalities University
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Priority to CN201711358048.XA priority patent/CN108043372A/en
Publication of CN107175089A publication Critical patent/CN107175089A/en
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Abstract

The present invention relates to a kind of iron-nickel magnetic microballoon of cobalt ions that adulterates and its application, belong to chemical material technical field.Main technical schemes are as follows：Cobalt ions and nickel ion are used in mixed way in a kind of iron nickel magnetic ball for the cobalt ions that adulterates, magnetic ball preparation process, the cobalt ions accounts for the 20 30% of cobalt ions and nickel ion total mole number, preferably 25%.The iron-nickel magnetic microballoon of doping cobalt ions synthesized by the present invention can be directly used for purifying and carry histidine-tagged target protein, without carrying out other modifications, such as antibody modification, and good dispersion in magnetic ball surface, and application effect is rapidly and efficiently, better than the iron-nickel magnetic microballoon without cobalt doped.The iron-nickel magnetic microballoon of cobalt ions that adulterates is suitable with the Ago-Gel separating property that the coordination of import combines nickel ion, can also realize Magnetic Isolation, can substitute import separation material.

Description

A kind of iron-nickel magnetic microballoon of cobalt ions that adulterates and its application
Technical field
The present invention relates to iron-nickel magnetic microballoon and its application of magnetic microsphere, more particularly to a kind of cobalt ions that adulterates.
Background technology
Magnetic macromolecular microsphere is a kind of novel magnetic materials that developed recently gets up, not only with common polymer microsphere Numerous characteristics also there is magnetic responsiveness, the method such as can not only be modified by copolymerization and surface and assign its surface functional group, also There can be guide function under additional magnetic fields.At present, magnetic composite microsphere is widely used in biomedical, cytology and divided From numerous areas such as engineerings.
The histidine-tagged production process for having been widely used for protein, the agarose for combining nickel ion using coordination coagulates Glue can adsorb histidine-tagged protein quickly and with high selectivity, and improve protein isolates and purifies speed, reduces egg The purifying cost of white matter, several functions protein completes merchandized handling and application using this production technology. In the production process of these functional proteins, the Ago-Gel price that coordination combines nickel ion is high, during isolating and purifying Shared production cost ratio is excessive, therefore, and exploitation is quick, protein separation method efficiently, inexpensive is always albumen The bottleneck of matter industry development.
The content of the invention
The present invention is high for the Ago-Gel price for solving coordination combination nickel ion present in prior art, pure separating There is provided a kind of iron-nickel magnetic microballoon for the cobalt ions that adulterates, Ke Yizhi for the excessive deficiency of shared production cost proportion during change Absorption histidine-tagged protein is connect, and magnetic microsphere is based on thermal decomposition principle and prepared using one kettle way, and production cost is low, doping The magnetic microsphere of cobalt ions is higher than the iron-nickel magnetic microballoon without ZnO thin film to the adsorption capacity of histidine-tagged protein.
The present invention inventive concept be：Iron, nickel, cobalt are the most frequently used ferromagnetic materials, may be used to magnetic microsphere system It is standby, it is that we study the performance impact of cation doping ion pair iron-nickel magnetic microballoon this；Found in research process, a small amount of cation doping Ion can improve adsorption capacity of the iron-nickel magnetic microballoon to histidine-tagged protein.
Technical scheme is specific as follows：In a kind of iron-nickel magnetic microballoon for the cobalt ions that adulterates, magnetic ball preparation process Cobalt ions and nickel ion are used in mixed way, the cobalt ions accounts for the 20-30% of cobalt ions and nickel ion total mole number, are preferably 25%.
Further, described cobalt ions is bivalent cation, and existence form is chloride, sulfate, nitrate.
Further, the grinding in advance of described cobalt ions, nickel ion, iron ion is uniform is used further to magnetic microsphere preparation.
Further, described iron-nickel magnetic microballoon is prepared using one kettle way, is comprised the following steps that：
A. soluble ferric iron salt, soluble nickel salt, soluble cobalt are mixed by certain mol proportion, makes original by constantly grinding Material is well mixed；Described soluble ferric iron salt, soluble nickel salt, soluble cobalt are the convenient source of this area, such as：Chlorination Iron, ferric sulfate, ferric nitrate, nickel chloride, nickel sulfate, nickel nitrate, cobalt chloride, cobaltous sulfate, cobalt nitrate.
B. ethylene glycol is stirred under the conditions of 200 DEG C, and is continually fed into the abundant deoxygenation of nitrogen；
C. under nitrogen protective condition, sodium acetate, monoethanolamine is added and is stirred, addition is pre-mixed uniform solubility Molysite, soluble nickel salt, soluble cobalt raw material, stirred under nitrogen atmosphere reaction 6h, are then cooled to room under nitrogen protection Temperature simultaneously stops reaction；
D. the product obtained in step c is washed with deionized 5-7 times, ethanol solution is washed 10-15 times, doping is made The iron-nickel magnetic microballoon of cobalt ions；
Another object of the present invention is the iron-nickel magnetic microballoon for protecting above-mentioned doping cobalt ions in protein purification Using the iron-nickel magnetic microballoon of doping cobalt ions may be directly applied to point of histidine-tagged protein without additionally modification after preparing From purifying.
Experiment through inventor confirms that the iron-nickel magnetic microballoon for the doping cobalt ions that the present invention is provided is to histidine-tagged egg There is high-affinity in vain, binding ability is better than the iron-nickel magnetic microballoon without ZnO thin film.
Magnetic microsphere is mixed with crude protein extract solution, by centrifugation or magnet adsorption, target protein absorption is micro- in magnetic On ball, by the magnetic microsphere with target protein with after appropriate buffer solution for cleaning, the elution buffer containing high concentration imidazoles can be used Liquid will be eluted with histidine-tagged target protein from magnetic microsphere.
The histidine-tagged principle for protein purification is：Histidine is the amino acid with heterocycle, each histidine Containing an imidazole group, imidazole group is combined by lone pair electrons with metallic ion coordination.Histidine-tagged is continuous 6 groups The function section of propylhomoserin, is combined with high-affinity with the metal ion of immobilization, so that isolating and purifying for protein.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) the iron-nickel magnetic microballoon of the doping cobalt ions synthesized by the present invention can be directly used for purifying histidine-tagged egg In vain, without carrying out other modifications, such as antibody modification on magnetic microsphere surface.
(2) the iron-nickel magnetic microballoon of doping cobalt ions produced by the present invention, good dispersion, and application effect is quickly high Effect, better than the iron-nickel magnetic microballoon without cobalt doped.
(3) it is of the present invention doping cobalt ions iron-nickel magnetic microballoon using one kettle way prepare, preparation method simply, into Sheet is low, easily carry out large-scale industrial production.
Brief description of the drawings
Fig. 1 detects figure for the dispersiveness and adsorptivity of the iron-nickel magnetic microballoon of doping cobalt ions；
Wherein：A, identical bacteria lysis supernatant, b, dispersiveness of the iron-nickel magnetic microballoon in water for the cobalt ions that adulterates Detection, c, aggregation of the iron-nickel magnetic microballoon in magnetic field and the absorption to albumen of the cobalt ions that adulterates.
Fig. 2 is influence schematic diagram of the cobalt ions substitute proportion to the protein adsorption capacity of magnetic microsphere；
Wherein：Ordinate is the percentage that residual protein accounts for former Tot Prot after magnetic microsphere is adsorbed.
The average grain diameter of the magnetic microsphere of Fig. 3 difference cobalt ions substitute proportions；
Fig. 4 is the contrast of elution result after five kinds of histidine-tagged GFP of magnetic microsphere junction belt；
Wherein, M：Protein molecular weight standard, 1：Centrifuged supernatant after bacteria lysis, 2：The magnetic of 15% substitution value is micro- Supernatant after ball absorption, 3：The magnetic microsphere eluted product of 15% substitution value, 4：After the magnetic microsphere absorption of 20% substitution value Supernatant, 5：The magnetic microsphere eluted product of 20% substitution value, 6：Supernatant, 7 after the magnetic microsphere absorption of 25% substitution value： The magnetic microsphere eluted product of 25% substitution value, 8：Supernatant, 9 after the magnetic microsphere absorption of 30% substitution value：30% substitution value Magnetic microsphere eluted product, 10：Supernatant, 11 after the magnetic microsphere absorption of 35% substitution value：The magnetic of 35% substitution value is micro- Ball eluted product.
After Fig. 5 is the magnetic microsphere combination His-tag-GFP of 25% substitution value, the contrast knot of imidazole concentration and eluting power Fruit detection (SDS-PAGE)；
Wherein, M：Protein molecular weight standard, 1：Centrifuged supernatant after bacteria lysis, 2：The magnetic microsphere of 25% substitution value Supernatant after absorption, 3-8：The elution effect of the different eluent of imidazoles final concentration compares, and wherein imidazoles final concentration is respectively： 0.20mol/L, 0.25mol/L, 0.3mol/L, 0.5mol/L, 1mol/L and 2mol/L.
Fig. 6 is coordinated the Ago-Gel (Ni- for combining nickel ion for the magnetic microsphere of 25% substitution value with import NTAagarose performance comparison result)；
Wherein, M：Protein molecular weight standard, 1：Not plus derivant bacterial lysate supernatant；2：Bacteria lysis after induction The supernatant of liquid；3：The direct sample preparation detection of thalline after induction；4：Centrifuged supernatant after Ago-Gel absorption；5：Ago-Gel is inhaled Attached eluate；6：Centrifuged supernatant after the magnetic microsphere absorption of 25% substitution value；7：The magnetic microsphere absorption of 25% substitution value Eluate afterwards.
Embodiment
The invention will be further described for 1-6 and specific embodiment below in conjunction with the accompanying drawings.Following examples are description Property, it is not limited, it is impossible to which protection scope of the present invention is limited with this.Chemical reagent and instrument that the present invention is applied Such as without special instruction, it can be bought from commercial channel.Preferably, three kinds of metal salts select cobalt nitrate, nickel sulfate, trichlorine respectively Change iron, be purchased from Chemical Reagent Co., Ltd., Sinopharm Group.
Embodiment 1
By cobalt nitrate, nickel sulfate in molar ratio 1:4 mixing (are designated as 20%, represent that cobalt ions accounts for cobalt ions total with nickel ion Molal quantity 20%), then mix with iron ion, be fully ground the preparation of the iron nickel magnetic ball for the cobalt ions that is used to adulterating after mixing.Magnetic Prepared by ball is to be based on thermal decomposition method principle, is prepared and completed using one kettle way, metal cation salt after mixing is added under nitrogen protection Enter into 200 DEG C of ethylene glycol solution (abundant deoxygenation, and add sodium acetate, monoethanolamine stir), keep nitrogen to protect Stirring reaction 6h under the conditions of shield, is then cooled to room temperature and stops reaction under nitrogen protection；By synthetic product deionized water Washing 5 times, ethanol solution is washed 5 times, and the iron nickel magnetic ball of 20% doping cobalt ions is made.
Embodiment 2
Cobalt nitrate, nickel sulfate are pressed to the mol ratio 1 of ion:3 mixing (are designated as 25%, represent that cobalt ions accounts for cobalt ions and nickel Ion total mole number 25%), then mix with iron ion, be fully ground the system of the iron nickel magnetic ball for the cobalt ions that is used to adulterating after mixing It is standby.Prepared by magnetic ball is to be based on thermal decomposition method principle, is prepared and completed using one kettle way, metal cation salt after mixing is protected in nitrogen Under be added in 200 DEG C of ethylene glycol solution (abundant deoxygenation, and add sodium acetate, monoethanolamine stir), keep nitrogen Stirring reaction 6h under the conditions of gas shielded, is then cooled to room temperature and stops reaction under nitrogen protection；By synthetic product spend from Sub- water washing 7 times, ethanol solution is washed 15 times, and the iron nickel magnetic ball of 25% doping cobalt ions is made.
Embodiment 3
Cobalt nitrate, nickel sulfate are pressed to the mol ratio 3 of ion:7 mixing (are designated as 30%, represent that cobalt ions accounts for cobalt ions and nickel Ion total mole number 30%), then mix with iron ion, be fully ground the system of the iron nickel magnetic ball for the cobalt ions that is used to adulterating after mixing It is standby.Prepared by magnetic ball is to be based on thermal decomposition method principle, is prepared and completed using one kettle way, metal cation salt after mixing is protected in nitrogen Under be added in 200 DEG C of ethylene glycol solution (abundant deoxygenation, and add sodium acetate, monoethanolamine stir), keep nitrogen Stirring reaction 6h under the conditions of gas shielded, is then cooled to room temperature and stops reaction under nitrogen protection；By synthetic product spend from Sub- water washing 6 times, ethanol solution is washed 10 times, and the iron nickel magnetic ball of 30% doping cobalt ions is made.
Embodiment 4
Cobalt nitrate, nickel sulfate are pressed to the mol ratio 7 of ion:13 mixing (are designated as 35%, represent that cobalt ions accounts for cobalt ions and nickel Ion total mole number 35%), then mix with iron ion, be fully ground the system of the iron nickel magnetic ball for the cobalt ions that is used to adulterating after mixing It is standby.Prepared by magnetic ball is to be based on thermal decomposition method principle, is prepared and completed using one kettle way, metal cation salt after mixing is protected in nitrogen Under be added in 200 DEG C of ethylene glycol solution (abundant deoxygenation, and add sodium acetate, monoethanolamine stir), keep nitrogen Stirring reaction 6h under the conditions of gas shielded, is then cooled to room temperature and stops reaction under nitrogen protection；By synthetic product spend from Sub- water washing 5 times, ethanol solution is washed 15 times, and the iron nickel magnetic ball of 35% doping cobalt ions is made.
Embodiment 5
Cobalt nitrate, nickel sulfate are pressed to the mol ratio 3 of ion:17 mixing (are designated as 15%, represent that cobalt ions accounts for cobalt ions and nickel Ion total mole number 15%), then mix with iron ion, be fully ground the system of the iron nickel magnetic ball for the cobalt ions that is used to adulterating after mixing It is standby.Prepared by magnetic ball is to be based on thermal decomposition method principle, is prepared and completed using one kettle way, metal cation salt after mixing is protected in nitrogen Under be added in 200 DEG C of ethylene glycol solution (abundant deoxygenation, and add sodium acetate, monoethanolamine stir), keep nitrogen Stirring reaction 6h under the conditions of gas shielded, is then cooled to room temperature and stops reaction under nitrogen protection；By synthetic product spend from Sub- water washing 7 times, ethanol solution is washed 15 times, and the iron nickel magnetic ball of 15% doping cobalt ions is made.
Embodiment 6
Cobalt nitrate, nickel sulfate are pressed to the mol ratio 1 of ion:1 mixing (is designated as 50%, represents that cobalt ions accounts for cobalt ions and nickel Ion total mole number 50%), then mix with iron ion, be fully ground the system of the iron nickel magnetic ball for the cobalt ions that is used to adulterating after mixing It is standby.Prepared by magnetic ball is to be based on thermal decomposition method principle, is prepared and completed using one kettle way, metal cation salt after mixing is protected in nitrogen Under be added in 200 DEG C of ethylene glycol solution (abundant deoxygenation, and add sodium acetate, monoethanolamine stir), keep nitrogen Stirring reaction 6h under the conditions of gas shielded, is then cooled to room temperature and stops reaction under nitrogen protection；By synthetic product spend from Sub- water washing 6 times, ethanol solution is washed 10 times, and the iron nickel magnetic ball of 50% doping cobalt ions is made.
Embodiment 7
Magnetic ball only is prepared using nickel sulfate, the cobalt ions that undopes (is designated as 0%, represent to be free of cobalt ions, as control), directly Connect and mixed with nickel sulfate with iron ion, being fully ground is used for the preparation for compareing iron nickel magnetic ball after mixing.Prepared by magnetic ball is based on heat Decomposition method principle, is prepared using one kettle way and completed, metal cation salt after mixing is added to 200 DEG C of second two under nitrogen protection In alcoholic solution (abundant deoxygenation, and add sodium acetate, monoethanolamine stir), stirring reaction under nitrogen protective condition is kept 6h, is then cooled to room temperature and stops reaction under nitrogen protection；7 times, ethanol solution are washed with deionized in synthetic product Washing 5 times, is made the control iron nickel magnetic ball without ZnO thin film.
Adulterate cobalt ions in iron nickel magnetic ball preparation process, can improve absorption of the magnetic ball to histidine-tagged protein；Its In, bivalent cation is used in mixed way cobalt ions and nickel ion, and cobalt ions accounts for bivalent cation (cobalt ions and nickel ion) and always rubbed Effect is good when the ratio of your number is 20-30%.
Contrast experiment is carried out by 25% magnetic ball of ZnO thin film ratio, by the magnetic ball that ZnO thin film ratio is 25% With containing bacteria lysis supernatant mix, centrifuged after being sufficiently mixed and supernatant and determine protein residues amount, after absorption Supernatant residual protein accounts for the 23.08% of former Tot Prot, and is 49.62% without cobalt ions control magnetic ball, 25% ZnO thin film The protein adsorption capacity of magnetic ball be doubled (see accompanying drawing 2).
ZnO thin film ratio for 15%-35% magnetic balls average grain diameter between 274.03nm-290.63nm (see accompanying drawing 3) protein absorption, is used equally for (see accompanying drawing 4, accompanying drawing 5).
The histidine-tagged protein adsorbed on iron nickel magnetic ball can be separated using the eluent containing imidazoles, complete mesh Mark isolating and purifying (see accompanying drawing 5) for albumen.The separating property of the histidine-tagged protein of the iron nickel magnetic ball of doping cobalt ions is with entering The coordination of mouth combines Ago-Gel (Ni-NTA agarose) performance of nickel ion quite (see accompanying drawing 6), and can realize magnetic Property separation, imported material can be substituted completely.

Claims (6)

1. it is a kind of adulterate cobalt ions iron-nickel magnetic microballoon, it is characterised in that be used in mixed way in magnetic ball preparation process cobalt ions with Nickel ion, the cobalt ions accounts for the 20-30% of cobalt ions and nickel ion total mole number.
2. the iron-nickel magnetic microballoon of a kind of cobalt ions that adulterates as claimed in claim 1, it is characterised in that described cobalt ions is accounted for Cobalt ions and the 25% of nickel ion total mole number.
3. a kind of iron-nickel magnetic microballoon of cobalt ions that adulterates as claimed in claim 1, it is characterised in that described cobalt ions is Bivalent cation, existence form is chloride, sulfate, nitrate.
4. a kind of iron-nickel magnetic microballoon of cobalt ions that adulterates as claimed in claim 1, it is characterised in that described cobalt ions, The grinding in advance of nickel ion, iron ion is uniform to be used further to magnetic microsphere preparation.
5. a kind of iron-nickel magnetic microballoon of cobalt ions that adulterates as claimed in claim 1, it is characterised in that described iron-nickel magnetic Microballoon is prepared using one kettle way, is comprised the following steps that：
A. soluble ferric iron salt, soluble nickel salt, soluble cobalt are mixed in certain molal quantity ratio, by constantly grinding Mill is well mixed raw material；
B. ethylene glycol is stirred under the conditions of 200 DEG C, and is continually fed into the abundant deoxygenation of nitrogen；
C. under nitrogen protective condition, sodium acetate, monoethanolamine is added and is stirred, addition is pre-mixed uniform soluble iron Salt, soluble nickel salt, soluble cobalt raw material, stirred under nitrogen atmosphere reaction 6h, are then cooled to room temperature under nitrogen protection And stop reaction；
D. the product obtained in step c is washed with deionized 5-7 times, ethanol solution is washed 10-15 time, obtained cation doping from The iron-nickel magnetic microballoon of son.
6. application of the iron-nickel magnetic microballoon as described in claim 1-5 is any in Separation of Proteins, it is characterised in that described Iron-nickel magnetic microballoon be directly adsorbed with histidine-tagged protein.
CN201710417423.7A 2017-06-06 2017-06-06 A kind of iron-nickel magnetic microballoon of cobalt ions that adulterates and its application Pending CN107175089A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108554412A (en) * 2018-05-11 2018-09-21 江西理工大学 A kind of preparation method and applications of large scale high porosity Fe doping photocatalyzing magnetic porous microsphere
CN109954517A (en) * 2019-04-28 2019-07-02 大连民族大学 A kind of Au@NH2- MIL-125 nanometers of enzyme catalysts and preparation method thereof
CN109999915A (en) * 2019-04-28 2019-07-12 大连民族大学 One kind being used for CO2The porphyryl metal-organic framework materials and preparation method thereof of cyclisation catalysis reaction
CN110038533A (en) * 2019-04-04 2019-07-23 深圳市南科征途有限公司 High magnetic particle diameter nanometer magnetic bead and preparation method thereof

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CN110038533A (en) * 2019-04-04 2019-07-23 深圳市南科征途有限公司 High magnetic particle diameter nanometer magnetic bead and preparation method thereof
CN109954517A (en) * 2019-04-28 2019-07-02 大连民族大学 A kind of Au@NH2- MIL-125 nanometers of enzyme catalysts and preparation method thereof
CN109999915A (en) * 2019-04-28 2019-07-12 大连民族大学 One kind being used for CO2The porphyryl metal-organic framework materials and preparation method thereof of cyclisation catalysis reaction
CN109999915B (en) * 2019-04-28 2021-12-21 大连民族大学 For CO2Porphyrin-based metal organic framework material for cyclization catalytic reaction and preparation method thereof

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