CN107173337A - A kind of prevention and controls for silkworm double cross original seed granulosis semina vertical transmission - Google Patents
A kind of prevention and controls for silkworm double cross original seed granulosis semina vertical transmission Download PDFInfo
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- CN107173337A CN107173337A CN201710462792.8A CN201710462792A CN107173337A CN 107173337 A CN107173337 A CN 107173337A CN 201710462792 A CN201710462792 A CN 201710462792A CN 107173337 A CN107173337 A CN 107173337A
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- silkworm
- seed
- double cross
- prevention
- silkworm seed
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 84
- 230000002265 prevention Effects 0.000 title claims abstract description 24
- 230000005570 vertical transmission Effects 0.000 title abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 20
- 208000015181 infectious disease Diseases 0.000 claims abstract description 15
- 238000005470 impregnation Methods 0.000 claims abstract description 9
- 230000009967 tasteless effect Effects 0.000 claims abstract description 6
- 230000003139 buffering effect Effects 0.000 claims abstract description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 20
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- RPAJSBKBKSSMLJ-DFWYDOINSA-N (2s)-2-aminopentanedioic acid;hydrochloride Chemical class Cl.OC(=O)[C@@H](N)CCC(O)=O RPAJSBKBKSSMLJ-DFWYDOINSA-N 0.000 claims description 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 2
- 229910017604 nitric acid Inorganic materials 0.000 claims description 2
- 201000010099 disease Diseases 0.000 abstract description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 12
- 235000013601 eggs Nutrition 0.000 abstract description 12
- 230000012447 hatching Effects 0.000 abstract description 12
- 238000011161 development Methods 0.000 abstract description 3
- 230000018109 developmental process Effects 0.000 abstract description 3
- 206010067482 No adverse event Diseases 0.000 abstract description 2
- 238000011534 incubation Methods 0.000 description 11
- 238000007598 dipping method Methods 0.000 description 10
- 238000010828 elution Methods 0.000 description 6
- 241000243190 Microsporidia Species 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 241001126828 Nosema bombycis Species 0.000 description 3
- 238000009366 sericulture Methods 0.000 description 3
- 244000048199 Hibiscus mutabilis Species 0.000 description 2
- 240000000249 Morus alba Species 0.000 description 2
- 235000008708 Morus alba Nutrition 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000005058 diapause Effects 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of prevention and controls for silkworm double cross original seed granulosis germinal infection, this comprises the following steps：Silkworm seed is without chilling treatment：Then silkworm seed is impregnated into 6~10min by silkworm seed in standing 15~24h at 20~30 DEG C in 47~48 DEG C of water after silkworm spawning；It is eluted to clear water tasteless after silkworm seed is taken out, is then dried；Silkworm seed is by chilling treatment：Silkworm seed is taken out from freezer, and several hours are stood at 15 20 DEG C as buffering, 6~10min of impregnation process in 47~50 DEG C of water is then placed in；It is eluted to clear water tasteless after silkworm seed is taken out, is then dried.The present invention is to the relative prevention effect of pebrine disease semina vertical transmission up to 90~97%, and Hatching Rate of Silkworm Eggs has no adverse effects up to more than 92% to Growth And Development of Silkworm.
Description
Technical field
The invention belongs to sericultural production field, and in particular to one kind is vertically passed for silkworm double cross original seed granulosis semina
The prevention and controls broadcast.
Background technology
Pebrine disease be the pathogenicity microsporidian infected silkworms such as nosema bombycis (Nosema bombycis) and
A kind of infectiousness silkworm epidemic disease occurred.The disease both can also make silkworm egg band poison through being infected under mulberry leaves food through germinal infection, because
This disease always extremely pay attention to by industry, is the unique object of silkworm egg quarantine.
China's silkworm and mulberry development of the 80s and 90s in last century is swift and violent, and silkworm egg production facility falls behind and technical management is extensive causes
Pebrine disease is very popular in China.In recent years, changing with sericulture region, production of hybrid seeds environment and management mode
Become, pebrine disease has revivable sign, heavy economic losses is brought to sericulture industry again.
Currently, the cultivated silkworm breed variety of popularization and application is all tetra hybrid, also known as common species on domestic sericultural production.It is profit
With hybrid vigour, four cultivated silkworm breed varieties are by taking the Hybrid that (A × B) × (C × D) mating pattern is bred as.Two product
Plant and first carry out two-way cross generation double cross original seed, right latter two double cross original seed is hybridized generation tetra hybrid.Therefore, breed double
Hand over the main task that original seed is original strain of mulberry silkworm.
Preventing and treating double cross original seed germinal infection is the important leverage of silkworm egg production safety.But, pebrine disease also lacks
Special treatment method, is currently mostly by examining microsporidian to the female moth of spawning under the microscope, being picked according to microscopy result
Except poisonous silkworm egg, so as to prevent and treat the germinal infection of pebrine disease.However, this method and the experience and technology water of the malicious personnel of inspection
It is flat relevant, easily cause erroneous judgement and fail to judge.Therefore, the new method for finding preventing and treating pebrine disease germinal infection is always sericulture
The emphasis direction of scientific research.
The content of the invention
For this not perfect present situation of prevention and controls of silkworm double cross original seed germinal infection, it is an object of the invention to carry
For a kind of prevention and controls for double cross original seed granulosis germinal infection, this method is real by handling double cross original seed
Now inactivate the purpose of nosema bombycis in double cross original seed.The present invention's is simple to operate, practical, and compensate for Microscopical Method For Detection may
The situation of failing to judge occurred, is the useful supplement of Microscopical Method For Detection, the silkworm egg after processing is realized nontoxic substantially.
The purpose of the present invention is achieved through the following technical solutions：
A kind of prevention and controls for silkworm double cross original seed granulosis germinal infection, comprise the following steps：
Silkworm seed is without chilling treatment：Silkworm seed is treated into the embryo of silkworm seed in standing 15~24h at 20~30 DEG C after silkworm spawning
Fetal hair, which is educated, to be formed the phase to germ band and does next step processing again；Then silkworm seed is impregnated into 6~10min in 47~48 DEG C of water, impregnated
When should ensure that silkworm seed and solution are uniform, fully contact；It is eluted to clear water tasteless after silkworm seed is taken out, is then dried；
Silkworm seed is by chilling treatment：Silkworm seed is taken out from freezer, and several hours are stood at 10-20 DEG C as buffering,
6~10min of impregnation process in 47~50 DEG C of water is then placed in, should ensure that silkworm seed is uniform with solution, fully contact during dipping；Will
Silkworm seed is eluted to tasteless after taking out with clear water, is then dried；
The silkworm seed is double cross original seed；
Described several hours are preferred 1-3 hours.
Described water, preferably comprises acid or hydrogen peroxide；
Described acid is hydrochloric acid, sulfuric acid, nitric acid or acetic acid etc.；
The water containing hydrochloric acid, its density is 1.075-1.092g/cm3。
What the inventive method was leaned on is that water temperature kills microsporidian, but is needed for silkworm double cross original seed, water temperature and dip time
Grope and select.Water temperature is low to be not enough to kill microsporidian, and water temperature over-high then has an impact to the incubation rate of silkworm seed；During dipping
Between be also in this way, the time it is short be not enough to kill microsporidian, impregnate long and have an impact to the incubation rate of silkworm seed.The present invention is to this
Performing creative labour has been paid in the determination of two features, has obtained suitable scope, achieves good effect.Meanwhile, this hair
Bright dilute acid soln impregnates, and is conducive to releasing silkworm seed diapause, improves incubation rate.
The present invention has the following advantages and effect relative to prior art：
1st, the present invention to the relative prevention effect of double cross original seed pebrine disease semina vertical transmission up to 90~97%.
2nd, method Hatching Rate of Silkworm Eggs of the invention has no adverse effects up to more than 92% to Growth And Development of Silkworm.
Brief description of the drawings
Fig. 1 is influence of the dipping temperature to the double cross original seed incubation rate without chilling treatment.
Fig. 2 is influence of the dipping temperature to the double cross original seed incubation rate through chilling treatment.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited
In this.
Embodiment 1
A kind of prevention and controls for silkworm double cross original seed granulosis germinal infection, comprise the following steps：
" Guangdong and Guangxi Provinces two " double cross original seed, by silkworm seed in protecting 20h at 25 DEG C after spawning, be then placed in 47 DEG C, density be
1.075g/cm3Hydrochloric acid solution in impregnation process 10min, then take out elution, dry, hasten the hatching of silkworms, hatch.Practical incubation rate reaches
94%, 3 ages silkworm microscopic examination, compared with untreated control, with respect to prevention effect up to 95%.
Calculation formula with respect to prevention effect is as follows：
With respect to prevention effect (%)=(the 100- treatment groups incidence of disease/control group incidence of disease) × 100
Embodiment 2
A kind of prevention and controls for silkworm double cross original seed granulosis germinal infection, comprise the following steps：
" Guangdong and Guangxi Provinces two " double cross original seed, by silkworm seed in protecting 20h at 25 DEG C after spawning, be then placed in 48 DEG C, density be
1.075g/cm3Hydrochloric acid solution in impregnation process 6min, then take out elution, dry, hasten the hatching of silkworms, hatch.Practical incubation rate reaches
93%, 3 ages silkworm microscopic examination, compared with untreated control, with respect to prevention effect up to 96%.
Embodiment 3
A kind of prevention and controls for silkworm double cross original seed granulosis germinal infection, comprise the following steps：
" Guangdong and Guangxi Provinces two " double cross original seed, by silkworm seed in protecting 20h at 25 DEG C after spawning, be then placed in 47.5 DEG C, density be
1.075g/cm3Hydrochloric acid solution in impregnation process 8min, then take out elution, dry, hasten the hatching of silkworms, hatch.Practical incubation rate reaches
94%, 3 ages silkworm microscopic examination, compared with untreated control, with respect to prevention effect up to 97%.
Embodiment 4
In the case of other operation identicals, the reality of different dipping temperature solution is done to the silkworm seed of the cottonrose hibiscus of double cross original seed 9,7 Hunan
Test, investigate dipping temperature to not refrigerating the influence of double cross original seed Hatching Rate of Silkworm Eggs, as a result see Fig. 1.As can be seen that liquid temperature is more than 48
DEG C, Hatching Rate of Silkworm Eggs is remarkably decreased, therefore the temperature upper limit of dipping solution selects 48 DEG C.
Embodiment 5
A kind of prevention and controls for silkworm double cross original seed granulosis germinal infection, comprise the following steps：
" Guangdong and Guangxi Provinces two " double cross original seed, it is stored refrigerated, silkworm seed is taken out from freezer and protects 2h under medium temperature (15 DEG C),
Be then placed in 47 DEG C, proportion be 1.092g/cm3Hydrochloric acid solution in impregnation process 10min, then take out elution, dry, urge
Blue or green, hatching.Practical incubation rate is up to the silkworm microscopic examination of 94%, 3 age, compared with untreated control, with respect to prevention effect up to 92%.
Embodiment 6
A kind of prevention and controls for silkworm double cross original seed granulosis germinal infection, comprise the following steps：
" Guangdong and Guangxi Provinces two " double cross original seed, it is stored refrigerated, silkworm seed is taken out in (15 DEG C) protection 2h of medium temperature, so from freezer
After be put into 50 DEG C, proportion be 1.092g/cm3Hydrochloric acid solution in impregnation process 6min, then take out elution, dry, hasten the hatching of silkworms, incubate
Change.Practical incubation rate is up to the silkworm microscopic examination of 93%, 3 age, compared with untreated control, with respect to prevention effect up to 93%.
Embodiment 7
A kind of prevention and controls for silkworm double cross original seed granulosis germinal infection, comprise the following steps：
" Guangdong and Guangxi Provinces two " double cross original seed, it is stored refrigerated, silkworm seed is taken out in (15 DEG C) protection 2h of medium temperature, so from freezer
After be put into 48 DEG C, proportion be 1.092g/cm3Hydrochloric acid solution in impregnation process 8min, then take out elution, dry, hasten the hatching of silkworms, incubate
Change.Practical incubation rate is up to the silkworm microscopic examination of 94%, 3 age, compared with untreated control, with respect to prevention effect up to 95%.
Embodiment 8
In the case of other operation identicals, the reality of different dipping temperature solution is done to the silkworm seed of the cottonrose hibiscus of double cross original seed 9,7 Hunan
Test, investigate influence of the dipping temperature to refrigeration double cross original seed Hatching Rate of Silkworm Eggs, as a result see Fig. 2.As can be seen that liquid temperature is more than 50
DEG C, Hatching Rate of Silkworm Eggs is remarkably decreased, therefore the temperature upper limit of dipping solution selects 50 DEG C.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (4)
1. a kind of prevention and controls for silkworm double cross original seed granulosis germinal infection, it is characterised in that comprise the following steps：
Silkworm seed is without chilling treatment：By silkworm seed in standing 15~24h at 20~30 DEG C after silkworm spawning, then by silkworm seed 47
6~10min is impregnated in~48 DEG C of water；It is eluted to clear water tasteless after silkworm seed is taken out, is then dried；
Silkworm seed is by chilling treatment：Silkworm seed is taken out from freezer, and several hours are stood at 10-20 DEG C as buffering, then
It is put into 6~10min of impregnation process in 47~50 DEG C of water；It is eluted to clear water tasteless after silkworm seed is taken out, is then dried.
2. method according to claim 1, it is characterised in that：Described water, contains acid or hydrogen peroxide.
3. method according to claim 2, it is characterised in that：Described acid is hydrochloric acid, sulfuric acid, nitric acid or acetic acid.
4. method according to claim 3, it is characterised in that：The water containing hydrochloric acid, its density is 1.075-1.092g/
cm3。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710462792.8A CN107173337B (en) | 2017-06-19 | 2017-06-19 | Method for preventing and treating vertical propagation of micro-particle disease embryo of silkworm double cross breeder seed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710462792.8A CN107173337B (en) | 2017-06-19 | 2017-06-19 | Method for preventing and treating vertical propagation of micro-particle disease embryo of silkworm double cross breeder seed |
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| Publication Number | Publication Date |
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| CN107173337A true CN107173337A (en) | 2017-09-19 |
| CN107173337B CN107173337B (en) | 2020-04-14 |
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|---|---|---|---|
| CN201710462792.8A Active CN107173337B (en) | 2017-06-19 | 2017-06-19 | Method for preventing and treating vertical propagation of micro-particle disease embryo of silkworm double cross breeder seed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757444A (en) * | 2019-03-25 | 2019-05-17 | 广东省农业科学院蚕业与农产品加工研究所 | A method of targeting heat treatment silkworm egg treats pebrine disease |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1212835A (en) * | 1997-09-30 | 1999-04-07 | 四川省农业科学院蚕业研究所 | Disinfactant for silkworm |
| CN1687767A (en) * | 2005-05-11 | 2005-10-26 | 苏州大学 | Diseased eggs of nosemosis in product eggs of domestic silkworm, and method for detecting rate of diseased eggs |
| CN101077138A (en) * | 2007-07-22 | 2007-11-28 | 唐珍发 | Several use method for adding drug on silkworm disease control |
-
2017
- 2017-06-19 CN CN201710462792.8A patent/CN107173337B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1212835A (en) * | 1997-09-30 | 1999-04-07 | 四川省农业科学院蚕业研究所 | Disinfactant for silkworm |
| CN1687767A (en) * | 2005-05-11 | 2005-10-26 | 苏州大学 | Diseased eggs of nosemosis in product eggs of domestic silkworm, and method for detecting rate of diseased eggs |
| CN101077138A (en) * | 2007-07-22 | 2007-11-28 | 唐珍发 | Several use method for adding drug on silkworm disease control |
Non-Patent Citations (1)
| Title |
|---|
| 河野太郎: "室温盐酸孵化法", 《国外农学－蚕业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757444A (en) * | 2019-03-25 | 2019-05-17 | 广东省农业科学院蚕业与农产品加工研究所 | A method of targeting heat treatment silkworm egg treats pebrine disease |
| CN109757444B (en) * | 2019-03-25 | 2022-03-04 | 广东省农业科学院蚕业与农产品加工研究所 | Method for treating silkworm pebrine by targeted heat treatment of silkworm seeds |
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| Publication number | Publication date |
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| CN107173337B (en) | 2020-04-14 |
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Application publication date: 20170919 Assignee: Guangdong Guangxin Seed Industry Co.,Ltd. Assignor: SERICULTURE & AGRI FOOD Research Institute GAAS Contract record no.: X2023980053696 Denomination of invention: A Prevention and Control Method for Vertical Transmission of Microparticle Disease in Embryos of Double Crossed Original Silkworm Granted publication date: 20200414 License type: Common License Record date: 20231222 |