CN107151652A - A kind of Vero cell culture mediums - Google Patents

A kind of Vero cell culture mediums Download PDF

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CN107151652A
CN107151652A CN201710527163.9A CN201710527163A CN107151652A CN 107151652 A CN107151652 A CN 107151652A CN 201710527163 A CN201710527163 A CN 201710527163A CN 107151652 A CN107151652 A CN 107151652A
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parts
sodium
acid
cell culture
culture mediums
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马荣华
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SUZHOU BEIKAI BIOCHEMICAL EQUIPMENT Co Ltd
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SUZHOU BEIKAI BIOCHEMICAL EQUIPMENT Co Ltd
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Abstract

The invention provides a kind of Vero cell culture mediums, including glucose, sodium acid carbonate, Sodium Pyruvate, human serum albumin, potassium chloride, anhydrous magnesium sulfate, sodium chloride, AMSP, PHA phytohemagglutinins, folic acid, inositol, niacinamide, anhydrous calcium chloride, ferric nitrate, succinic acid, sodium succinate, D calcium pantothenates, choline tartrate, riboflavin, thiamine hydrochloride, pyridoxine hydrochloride, reduced glutathione, cholesterol, ascorbic acid phosphoric acid esters, makrolon, phenol red sodium and deionized water.Vero cell culture mediums of the present invention, its component is reasonable, it is nutritious, rich in growth factor, more nutrients, high cell growth speed can be provided for Vero cell culture, cell state is good, sharpness of border, Microscopic observation is bright, split coil method is more, form and decentralization are good, culture and research for Vero cells.

Description

A kind of Vero cell culture mediums
Technical field
The invention belongs to biological technology application, in particular it relates to a kind of Vero cell culture mediums.
Background technology
Cell culture is a biological important technology with healthy Related Research Domain.With the rapid hair of life science Exhibition, current cell culture turns into the important base of the disciplinary studies such as cell biology, molecular biology, science of heredity and immunology Plinth.In order to further investigated cell vegetative activity rule, have the pathology of related disorders and the pharmacology of medicine（Toxicity）Mechanism, exploitation tool There are different targetedly cell culture processes significant.
Vero cells are that Japanese scholars are built for 1962 from cercopithecus aethiops (cercopithecus aethiops monkey) kidney Vertical MK cells system.The origin of its title is taken from Esperanto two before VERDE the first two of " green " word letter and " kidney " word Individual letter is combined, but does not allow VERE to exist in Esperanto, and need to be ended up with " O ", therefore is named as " Vero ".Its 93rd generation The U.S. NIH allergy and tropic virus research department of Infectious Disease Research Institute are brought to, the 113rd generation was submitted to ATCC, and numbering is ATCC NO.CCL-81。
In addition to conventional scientific research needs, identify that Vero cells have karyogy stable, do not have by comprehensively research Exogenous factor pollutes the advantage with oncogenicity, complies fully with codes of the WHO in 1987 on the passage cell for biological products It is required that, and can be used as the matrix of production of vaccine by WHO approvals.Then, Vero cells polio inactivated vaccine, polio Live vaccine, Vero cell rabies are developed and granted production in France in succession.Therefore, the application of Vero cells Prospect and demand are very big.
The content of the invention
Goal of the invention：The invention provides a kind of Vero cell culture mediums, the in vitro culture of Vero cells is exclusively used in.
Technical scheme：The invention provides a kind of Vero cell culture mediums, by weight part, including following components：Portugal 80-90 parts of grape sugar, 10-20 parts of sodium acid carbonate, 8-18 parts of Sodium Pyruvate, 2-10 parts of human serum albumin, 30-50 parts of potassium chloride, 5-12 parts of anhydrous magnesium sulfate, 60-80 parts of sodium chloride, 8-16 parts of AMSP, PHA- phytohemagglutinins 0.2-1 Part, 0.2-0.6 parts of folic acid, 0.5-1.5 parts of inositol, 0.2-0.8 parts of niacinamide, 20-40 parts of anhydrous calcium chloride, ferric nitrate 0.1- 0.5 part, 5-10 parts of succinic acid, 9-18 parts of sodium succinate, 0.2-0.8 parts of D-VB5 calcium, 0.5-1 parts of choline tartrate, core yellow Plain 0.1-0.3 parts, 0.1-0.5 parts of thiamine hydrochloride, 0.1-0.6 parts of pyridoxine hydrochloride, 0.1-0.8 parts of reduced glutathione, courage 1-5 parts of sterol, 0.5-1.2 parts of ascorbic acid phosphoric acid esters, 0.3-1.5 parts of makrolon, phenol red sodium 0.8-1.5 parts and deionized water 100 parts.
Further, above-mentioned Vero cell culture mediums, by weight part, including following components：Glucose 82-88 Part, 12-25 parts of sodium acid carbonate, 10-16 parts of Sodium Pyruvate, 5-9 parts of human serum albumin, 35-45 parts of potassium chloride, anhydrous magnesium sulfate 6-10 parts, 70-78 parts of sodium chloride, 10-14 parts of AMSP, 0.4-0.8 parts of PHA- phytohemagglutinins, folic acid 0.3-0.5 parts, 0.8-1.2 parts of inositol, 0.3-0.6 parts of niacinamide, 25-35 parts of anhydrous calcium chloride, 0.2-0.4 parts of ferric nitrate, fourth 6-8 parts of diacid, 12-16 parts of sodium succinate, 0.3-0.6 parts of D-VB5 calcium, 0.6-0.8 parts of choline tartrate, riboflavin 0.1- 0.3 part, 0.2-0.4 parts of thiamine hydrochloride, 0.3-0.5 parts of pyridoxine hydrochloride, 0.2-0.6 parts of reduced glutathione, cholesterol 2-4 Part, 0.6-1 parts of ascorbic acid phosphoric acid esters, 0.4-0.9 parts of makrolon, phenol red sodium 1-1.3 parts and 100 parts of deionized water.
As the present invention a kind of preferred embodiment, above-mentioned Vero cell culture mediums, by weight part, including with the following group Point：86 parts of glucose, 18 parts of sodium acid carbonate, 12 parts of Sodium Pyruvate, 6 parts of human serum albumin, 40 parts of potassium chloride, anhydrous magnesium sulfate 8 parts, 75 parts of sodium chloride, 12 parts of AMSP, 0.5 part of PHA- phytohemagglutinins, 0.4 part of folic acid, 1 part of inositol, 0.5 part of niacinamide, 30 parts of anhydrous calcium chloride, 0.3 part of ferric nitrate, 7 parts of succinic acid, 14 parts of sodium succinate, 0.5 part of D-VB5 calcium, 0.7 part of choline tartrate, 0.2 part of riboflavin, 0.3 part of thiamine hydrochloride, 0.4 part of pyridoxine hydrochloride, reduced glutathione 0.5 Part, 3 parts of cholesterol, 0.8 part of ascorbic acid phosphoric acid esters, 0.8 part of makrolon, 1.2 parts of phenol red sodium and 100 parts of deionized water.
Further, above-mentioned Vero cell culture mediums, in addition to 3-8 parts of amino acid, the amino acid is with components by weight percent Meter, it is composed of the following components：5-10 parts of L- R-genes, 3-8 parts of L- hydrochloric acid cystines, 3-6 parts of Serine, glycine 1-5 parts, 4-6 parts of L- histidine monohydrochlorides, 8-20 parts of ILE, 7-18 parts of L-Leu, 10-20 parts of LYS, 1-6 parts of METHIONINE, 2-8 parts of L-phenylalanine, 5-15 parts of L-threonine, 1-3 parts of L-Trp, 5-9 parts of TYR and 8-12 parts of Valine.
Further, above-mentioned Vero cell culture mediums, in addition to 2-5 parts of confactors, the confactor is with weight Component meter, it is composed of the following components：2-10 parts of recombination human basic fibroblast growth factor, 3-8 parts of interleukin-22, interleukin 1-4 parts of 4 3-6 parts, 1-5 parts of interleukin-11 4,3-6 parts of IFN-γ and neuroleukin.
Further, above-mentioned Vero cell culture mediums, include volume ratio 10-30% hyclone.
Further, above-mentioned Vero cell culture mediums, by weight part, in addition to 0.006 part of penicillin and 0.01 The streptomysin of part.
Further, above-mentioned Vero cell culture mediums, the penicillin is selected from 10000U/ml, and the streptomysin is selected from 10000μg/ml。
Beneficial effect：Vero cell culture mediums of the present invention, its component is rationally, nutritious, rich in growth factor, More nutrients can be provided for Vero cell culture, high cell growth speed, cell state is good, sharpness of border, Jing Xiaguan Examine that more bright, split coil method, form and decentralization are good, culture and research for Vero cells.
Embodiment
Below will be by several specific embodiments, the present invention is furture elucidated, these embodiments simply to illustrate that problem, It is not a kind of limitation.
Embodiment 1
A kind of Vero cell culture mediums, by weight part, including following components：80 parts of glucose, 10 parts of sodium acid carbonate, third 8 parts of ketone acid sodium, 2 parts of human serum albumin, 30 parts of potassium chloride, 5 parts of anhydrous magnesium sulfate, 60 parts of sodium chloride, AMSP 8 parts, 0.2 part of PHA- phytohemagglutinins, 0.2 part of folic acid, 0.5 part of inositol, 0.2 part of niacinamide, 20 parts of anhydrous calcium chloride, 0.1 part of ferric nitrate, 5 parts of succinic acid, 9 parts of sodium succinate, 0.2 part of D-VB5 calcium, 0.5 part of choline tartrate, riboflavin 0.1 Part, 0.1 part of thiamine hydrochloride, 0.1 part of pyridoxine hydrochloride, 0.1 part of reduced glutathione, 1 part of cholesterol, ascorbic acid phosphoric acid esters 100 parts of 0.5 part, 0.3 part of makrolon, 0.8 part of phenol red sodium and deionized water.
In addition, above-mentioned Vero cell culture mediums also include 3 parts of amino acid, the amino acid by weight part, by following Component is constituted：5 parts of L- R-genes, 3 parts of L- hydrochloric acid cystine, 3 parts of Serine, 1 part of glycine, L- histidine monohydrochlorides 4 parts, 8 parts of ILE, 7 parts of L-Leu, 10 parts of LYS, 1 part of METHIONINE, 2 parts of L-phenylalanine, L- 8 parts of 5 parts of threonine, 1 part of L-Trp, 5 parts of TYR and Valine.
Again, above-mentioned Vero cell culture mediums also include 2 parts of confactors, the confactor by weight part, by Following components is constituted：2 parts of recombination human basic fibroblast growth factor, 3 parts of interleukin-22,3 parts of IL-4, interleukin-11 4 1 part of 1 part, 3 parts of IFN-γ and neuroleukin.
In addition, above-mentioned Vero cell culture mediums, include the hyclone of volume ratio 10%.And above-mentioned Vero is thin Born of the same parents' culture medium, by weight part, in addition to 0.006 part of penicillin and 0.01 part of streptomysin.Wherein, the penicillin is selected from 10000U/ml, the streptomysin is selected from 10000 μ g/ml.
Embodiment 2
A kind of Vero cell culture mediums, by weight part, including following components：90 parts of glucose, 20 parts of sodium acid carbonate, third 18 parts of ketone acid sodium, 10 parts of human serum albumin, 50 parts of potassium chloride, 12 parts of anhydrous magnesium sulfate, 80 parts of sodium chloride, anhydrous phosphoric acid dihydro 16 parts of sodium, 1 part of PHA- phytohemagglutinins, 0.6 part of folic acid, 1.5 parts of inositol, 0.8 part of niacinamide, 40 parts of anhydrous calcium chloride, 0.5 part of ferric nitrate, 10 parts of succinic acid, 18 parts of sodium succinate, 0.8 part of D-VB5 calcium, 1 part of choline tartrate, riboflavin 0.3 Part, 0.5 part of thiamine hydrochloride, 0.6 part of pyridoxine hydrochloride, 0.8 part of reduced glutathione, 5 parts of cholesterol, ascorbic acid phosphoric acid esters 100 parts of 1.2 parts, 1.5 parts of makrolon, 1.5 parts of phenol red sodium and deionized water.
In addition, above-mentioned Vero cell culture mediums also include 8 parts of amino acid, the amino acid by weight part, by following Component is constituted：10 parts of L- R-genes, 8 parts of L- hydrochloric acid cystine, 6 parts of Serine, 5 parts of glycine, L- hydrochloric acid group ammonia 6 parts of acid, 20 parts of ILE, 18 parts of L-Leu, 20 parts of LYS, 6 parts of METHIONINE, L-phenylalanine 8 Part, 15 parts of L-threonine, 3 parts of L-Trp, 9 parts of TYR and 12 parts of Valine.
Again, above-mentioned Vero cell culture mediums also include 5 parts of confactors, the confactor by weight part, by Following components is constituted：10 parts of recombination human basic fibroblast growth factor, 8 parts of interleukin-22,6 parts of IL-4, interleukin 4 parts of 14 5 parts, 6 parts of IFN-γ and neuroleukin.
In addition, above-mentioned Vero cell culture mediums, include the hyclone of volume ratio 30%.And above-mentioned Vero is thin Born of the same parents' culture medium, by weight part, in addition to 0.006 part of penicillin and 0.01 part of streptomysin.Wherein, the penicillin is selected from 10000U/ml, the streptomysin is selected from 10000 μ g/ml.
Embodiment 3
A kind of Vero cell culture mediums, by weight part, including following components：82 parts of glucose, 12 parts of sodium acid carbonate, third 10 parts of ketone acid sodium, 5 parts of human serum albumin, 35 parts of potassium chloride, 6 parts of anhydrous magnesium sulfate, 70 parts of sodium chloride, AMSP 10 parts, 0.4 part of PHA- phytohemagglutinins, 0.3 part of folic acid, 0.8 part of inositol, 0.3 part of niacinamide, 25 parts of anhydrous calcium chloride, 0.2 part of ferric nitrate, 6 parts of succinic acid, 12 parts of sodium succinate, 0.3 part of D-VB5 calcium, 0.6 part of choline tartrate, riboflavin 0.1 Part, 0.2 part of thiamine hydrochloride, 0.3 part of pyridoxine hydrochloride, 0.2 part of reduced glutathione, 2 parts of cholesterol, ascorbic acid phosphoric acid esters 100 parts of 0.6 part, 0.4 part of makrolon, 1 part of phenol red sodium and deionized water.
In addition, above-mentioned Vero cell culture mediums also include 3 parts of amino acid, the amino acid by weight part, by following Component is constituted：5 parts of L- R-genes, 3 parts of L- hydrochloric acid cystine, 3 parts of Serine, 1 part of glycine, L- histidine monohydrochlorides 4 parts, 8 parts of ILE, 7 parts of L-Leu, 10 parts of LYS, 1 part of METHIONINE, 2 parts of L-phenylalanine, L- 8 parts of 5 parts of threonine, 1 part of L-Trp, 5 parts of TYR and Valine.
Again, above-mentioned Vero cell culture mediums also include 2 parts of confactors, the confactor by weight part, by Following components is constituted：2 parts of recombination human basic fibroblast growth factor, 3 parts of interleukin-22,3 parts of IL-4, interleukin-11 4 1 part of 1 part, 3 parts of IFN-γ and neuroleukin.
In addition, above-mentioned Vero cell culture mediums, include the hyclone of volume ratio 10%.And above-mentioned Vero is thin Born of the same parents' culture medium, by weight part, in addition to 0.006 part of penicillin and 0.01 part of streptomysin.Wherein, the penicillin is selected from 10000U/ml, the streptomysin is selected from 10000 μ g/ml.
Embodiment 4
A kind of Vero cell culture mediums, by weight part, including following components：88 parts of glucose, 25 parts of sodium acid carbonate, third 16 parts of ketone acid sodium, 9 parts of human serum albumin, 45 parts of potassium chloride, 10 parts of anhydrous magnesium sulfate, 78 parts of sodium chloride, AMSP 14 parts, 0.8 part of PHA- phytohemagglutinins, 0.5 part of folic acid, 1.2 parts of inositol, 0.6 part of niacinamide, 35 parts of anhydrous calcium chloride, 0.4 part of ferric nitrate, 8 parts of succinic acid, 16 parts of sodium succinate, 0.6 part of D-VB5 calcium, 0.8 part of choline tartrate, riboflavin 0.3 Part, 0.4 part of thiamine hydrochloride, 0.5 part of pyridoxine hydrochloride, 0.6 part of reduced glutathione, 4 parts of cholesterol, ascorbic acid phosphoric acid esters 100 parts of 1 part, 0.9 part of makrolon, 1.3 parts of phenol red sodium and deionized water.
In addition, above-mentioned Vero cell culture mediums also include 8 parts of amino acid, the amino acid by weight part, by following Component is constituted：10 parts of L- R-genes, 8 parts of L- hydrochloric acid cystine, 6 parts of Serine, 5 parts of glycine, L- hydrochloric acid group ammonia 6 parts of acid, 20 parts of ILE, 18 parts of L-Leu, 20 parts of LYS, 6 parts of METHIONINE, L-phenylalanine 8 Part, 15 parts of L-threonine, 3 parts of L-Trp, 9 parts of TYR and 12 parts of Valine.
Again, above-mentioned Vero cell culture mediums also include 5 parts of confactors, the confactor by weight part, by Following components is constituted：10 parts of recombination human basic fibroblast growth factor, 8 parts of interleukin-22,6 parts of IL-4, interleukin 4 parts of 14 5 parts, 6 parts of IFN-γ and neuroleukin.
In addition, above-mentioned Vero cell culture mediums, include the hyclone of volume ratio 30%.Also, above-mentioned Vero is thin Born of the same parents' culture medium, by weight part, in addition to 0.006 part of penicillin and 0.01 part of streptomysin.Wherein, the penicillin is selected from 10000U/ml, the streptomysin is selected from 10000 μ g/ml.
Embodiment 5
A kind of Vero cell culture mediums, by weight part, including following components：86 parts of glucose, 18 parts of sodium acid carbonate, third 12 parts of ketone acid sodium, 6 parts of human serum albumin, 40 parts of potassium chloride, 8 parts of anhydrous magnesium sulfate, 75 parts of sodium chloride, AMSP 12 Part, 0.5 part of PHA- phytohemagglutinins, 0.4 part of folic acid, 1 part of inositol, 0.5 part of niacinamide, 30 parts of anhydrous calcium chloride, nitric acid 0.3 part of iron, 7 parts of succinic acid, 14 parts of sodium succinate, 0.5 part of D-VB5 calcium, 0.7 part of choline tartrate, 0.2 part of riboflavin, salt 0.3 part of allithiamine, 0.4 part of pyridoxine hydrochloride, 0.5 part of reduced glutathione, 3 parts of cholesterol, ascorbic acid phosphoric acid esters 0.8 Part, 0.8 part of makrolon, 1.2 parts of phenol red sodium and 100 parts of deionized water.
In addition, above-mentioned Vero cell culture mediums also include 5 parts of amino acid, the amino acid by weight part, by following Component is constituted：8 parts of L- R-genes, 6 parts of L- hydrochloric acid cystine, 5 parts of Serine, 3 parts of glycine, L- histidine monohydrochlorides 5 parts, 12 parts of ILE, 10 parts of L-Leu, 15 parts of LYS, 3 parts of METHIONINE, 4 parts of L-phenylalanine, 9 parts of 9 parts of L-threonine, 2 parts of L-Trp, 6 parts of TYR and Valine.
Again, above-mentioned Vero cell culture mediums also include 4 parts of confactors, the confactor by weight part, by Following components is constituted：8 parts of recombination human basic fibroblast growth factor, 6 parts of interleukin-22,4 parts of IL-4, interleukin-11 4 2 parts of 3 parts, 5 parts of IFN-γ and neuroleukin.
In addition, above-mentioned Vero cell culture mediums, include the hyclone of volume ratio 25%.Also, above-mentioned Vero is thin Born of the same parents' culture medium, by weight part, in addition to 0.006 part of penicillin and 0.01 part of streptomysin.Wherein, the penicillin is selected from 10000U/ml, the streptomysin is selected from 10000 μ g/ml.
Described above is only several embodiments of invention, it is noted that for those skilled in the art For, on the premise of inventive principle is not departed from, some improvement can also be made, these improvement also should be regarded as the protection of the present invention Scope.

Claims (8)

1. a kind of Vero cell culture mediums, it is characterised in that：By weight part, including following components：80-90 parts of glucose, 10-20 parts of sodium acid carbonate, 8-18 parts of Sodium Pyruvate, 2-10 parts of human serum albumin, 30-50 parts of potassium chloride, anhydrous magnesium sulfate 5- 12 parts, 60-80 parts of sodium chloride, 8-16 parts of AMSP, 0.2-1 parts of PHA- phytohemagglutinins, folic acid 0.2- 0.6 part, 0.5-1.5 parts of inositol, 0.2-0.8 parts of niacinamide, 20-40 parts of anhydrous calcium chloride, 0.1-0.5 parts of ferric nitrate, succinic acid 5-10 parts, 9-18 parts of sodium succinate, 0.2-0.8 parts of D-VB5 calcium, 0.5-1 parts of choline tartrate, 0.1-0.3 parts of riboflavin, salt It is 0.1-0.5 parts of allithiamine, 0.1-0.6 parts of pyridoxine hydrochloride, 0.1-0.8 parts of reduced glutathione, 1-5 parts of cholesterol, anti-bad .5-1.2 parts of hematic acid phosphoesterase 30,0.3-1.5 parts of makrolon, phenol red sodium 0.8-1.5 parts and 100 parts of deionized water.
2. Vero cell culture mediums according to claim 1, it is characterised in that：By weight part, including following components： 82-88 parts of glucose, 12-25 parts of sodium acid carbonate, 10-16 parts of Sodium Pyruvate, 5-9 parts of human serum albumin, potassium chloride 35-45 Part, 6-10 parts of anhydrous magnesium sulfate, 70-78 parts of sodium chloride, 10-14 parts of AMSP, PHA- phytohemagglutinins 0.4-0.8 parts, 0.3-0.5 parts of folic acid, 0.8-1.2 parts of inositol, 0.3-0.6 parts of niacinamide, 25-35 parts of anhydrous calcium chloride, nitric acid 0.2-0.4 parts of iron, 6-8 parts of succinic acid, 12-16 parts of sodium succinate, 0.3-0.6 parts of D-VB5 calcium, choline tartrate 0.6-0.8 Part, 0.1-0.3 parts of riboflavin, 0.2-0.4 parts of thiamine hydrochloride, 0.3-0.5 parts of pyridoxine hydrochloride, reduced glutathione 0.2- 0.6 part, 2-4 parts of cholesterol, 0.6-1 parts of ascorbic acid phosphoric acid esters, 0.4-0.9 parts of makrolon, phenol red sodium 1-1.3 parts and go from Sub- 100 parts of water.
3. Vero cell culture mediums according to claim 1, it is characterised in that：By weight part, including following components： 86 parts of glucose, 18 parts of sodium acid carbonate, 12 parts of Sodium Pyruvate, 6 parts of human serum albumin, 40 parts of potassium chloride, anhydrous magnesium sulfate 8 Part, 75 parts of sodium chloride, 12 parts of AMSP, 0.5 part of PHA- phytohemagglutinins, 0.4 part of folic acid, 1 part of inositol, 0.5 part of niacinamide, 30 parts of anhydrous calcium chloride, 0.3 part of ferric nitrate, 7 parts of succinic acid, 14 parts of sodium succinate, 0.5 part of D-VB5 calcium, 0.7 part of choline tartrate, 0.2 part of riboflavin, 0.3 part of thiamine hydrochloride, 0.4 part of pyridoxine hydrochloride, reduced glutathione 0.5 Part, 3 parts of cholesterol, 0.8 part of ascorbic acid phosphoric acid esters, 0.8 part of makrolon, 1.2 parts of phenol red sodium and 100 parts of deionized water.
4. the Vero cell culture mediums according to claim any one of 1-3, it is characterised in that：Also include 3-8 parts of amino acid, The amino acid is by weight part, composed of the following components：5-10 parts of L- R-genes, 3-8 parts of L- hydrochloric acid cystines, 3-6 parts of Serine, 1-5 parts of glycine, 4-6 parts of L- histidine monohydrochlorides, 8-20 parts of ILE, 7-18 parts of L-Leu, 10-20 parts of LYS, 1-6 parts of METHIONINE, 2-8 parts of L-phenylalanine, 5-15 parts of L-threonine, L-Trp 1- 8-12 parts of 3 parts, 5-9 parts of TYR and Valine.
5. Vero cell culture mediums according to claim 4, it is characterised in that：Also include 2-5 parts of confactors, it is described auxiliary Help the factor by weight part, it is composed of the following components：2-10 parts of recombination human basic fibroblast growth factor, interleukin-22 1-4 parts of 3-8 parts, 3-6 parts of IL-4,1-5 parts of interleukin-11 4,3-6 parts of IFN-γ and neuroleukin.
6. Vero cell culture mediums according to claim 5, it is characterised in that：Also include volume ratio 10-30% tire ox blood Clearly.
7. Vero cell culture mediums according to claim 6, it is characterised in that：By weight part, in addition to 0.006 part Penicillin and 0.01 part of streptomysin.
8. Vero cell culture mediums according to claim 7, it is characterised in that：The penicillin is selected from 10000U/ml, institute State streptomysin and be selected from 10000 μ g/ml.
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CN110616183A (en) * 2019-09-23 2019-12-27 山东甲骨文生物科技有限公司 Low-serum culture medium for Vero cell culture and corresponding virus production

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CN110564672A (en) * 2019-09-23 2019-12-13 山东甲骨文生物科技有限公司 Culture medium for Vero cell low-serum culture
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