CN107151646A - A kind of active bio battery construction method based on electroctyte - Google Patents

A kind of active bio battery construction method based on electroctyte Download PDF

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CN107151646A
CN107151646A CN201710353182.4A CN201710353182A CN107151646A CN 107151646 A CN107151646 A CN 107151646A CN 201710353182 A CN201710353182 A CN 201710353182A CN 107151646 A CN107151646 A CN 107151646A
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electroctyte
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nano
cell
solution
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CN107151646B (en
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贺健康
李涤尘
张兵
唐昊煜
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Xian Jiaotong University
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    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
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Abstract

A kind of active bio battery construction method based on electroctyte, first the separating charged powered organ of fish, extracts electroctyte, forms the mixed solution of electroctyte suspension or electroctyte and hydrogel；The biomaterial of micro-nano fiber manufacture and the mixed solution of conductive material are configured to, uniform biological conductive Material cladding solution is prepared；Biological conductive Material cladding solution is manufactured into by the support with micro-nano fiber structure using electrostatic spinning or micro-nano 3D printing technique, the mixed solution of electroctyte suspension or electroctyte and hydrogel is planted or is printed upon on the support with micro-nano fiber structure, obtain the complex structure body of micro-nano structure and electroctyte, cultivate in vitro, make cell according to the direction oriented growth and propagation of fiber, realize cell tandem；Winding up or superposition are finally carried out, the three-dimensional active bio battery structure of acquisition, the present invention has the advantages such as cleaning, efficient, strong adaptability.

Description

A kind of active bio battery construction method based on electroctyte
Technical field
The present invention relates to biology manufacture and field of new energy technologies, and in particular to a kind of active bio based on electroctyte Battery construction method.
Background technology
With the development of society, the mankind increase the demand of the energy year by year.At present, the main body of Energy Mix be still oil, The traditional energies such as coal.This non-renewable energy resources not only limited amount, and more serious problem of environmental pollution can be brought.Cause This, finds a kind of sustainable energy of clean and effective, gradually reduces and break away from the dependence to oil, coal resources, be to need solution badly Social concern certainly.The high pressure that the powered fish such as electric eel, the electric ray existed in nature can produce hundreds of volts is used to prey on and prevent Defend.In its musculature, millions of electroctytes are dispersed with, each cell can produce tens millivolts of voltage. Unique polarity between cell makes it mutually be in series, and realizes the output of high voltage electric energy.Although the powered fish of existing research and utilization Organ realizes external electric energy output, and drives simple electric elements, but not yet has the activity electricity based on biological growth principle The research report in pond.
The content of the invention
In order to overcome the shortcoming of above-mentioned prior art, it is an object of the present invention to provide a kind of active bio based on electroctyte Battery construction method, the biobattery of structure have it is renewable, be easy to et al. Ke, cleanliness without any pollution, underwater environment adaptability The advantage such as strong, can be widely used for the energy driving in the fields such as biochip, water environment electronic device, flexible robot.
In order to achieve the above object, the technical scheme taken of the present invention is：
A kind of active bio battery construction method based on electroctyte, comprises the following steps：
1) powered fish are chosen, separating charged organ extracts the electroctyte inside organ, forms electroctyte suspension Or the mixed solution of electroctyte and hydrogel, electroctyte concentration is 1 × 105To 1 × 107Individual/ml, beats as electroctyte The material of print/extrusion molding；
2) biomaterial of micro-nano fiber manufacture and the mixed solution of conductive material, first configuration quality fraction are configured to For 1% to 15% biomaterial solution, then adding conductive material makes the concentration of conductive material be 10mg/ml to 100mg/ Ml, uniform biological conductive Material cladding solution, the printing for conductive-nano-fibers are formed by dissolving or ultrasonic disperse；
3) biological conductive Material cladding solution is manufactured into using electrostatic spinning or micro-nano 3D printing technique fine with micro-nano The support of structure is tieed up, the growth for electroctyte is sought connections with and oriented, after disinfecting, the electroctyte of preparation is suspended The mixed solution plantation of liquid or electroctyte and hydrogel is printed upon on the support with micro-nano fiber structure, obtains micro-nano knot The complex structure body of structure and electroctyte；
4) complex structure body of micro-nano structure and cell is cultivated in vitro, makes cell according to the direction oriented growth of fiber With propagation, cell tandem is realized；
5) it will realize that the micro-nano structure of cell tandem carries out winding up with the complex structure body of cell or is superimposed, and obtain three-dimensional Active bio battery structure, is then placed in the dynamic biological inside reactor manufactured by insulating materials and is cultivated, and forms stable Electric energy output.
Described step 1) in powered fish be electric eel or electric ray.
Described step 1) in hydrogel be collagen, fibrin or gelatin.
Described step 2) in conductive material include Nano Silver, conducting polymer, CNT, graphene or they Mixture, biomaterial includes polyethylene glycol oxide, polycaprolactone, PLA, chitosan, fibroin albumen or their mixture.
Described step 5) in insulating materials be silicon rubber or lucite.
The described active bio battery construction method based on electroctyte is carried out in an aseptic environment.
Described active bio battery structure includes electricity generation module 3 and battery case 4, and electricity generation module 3 is by electroctyte 1st, micro-nano conductive fiber support 2 and cell nutrient solution 5 are constituted, and electroctyte 1 is the base unit that biobattery produces electric energy；Hair It is connected in series or parallel between electric cell 1 by micro-nano conductive fiber support 2, electroctyte 1 is survived in cell nutrient solution 5 Interior, the existence of electroctyte 1 needed nutrient matter is provided by cell nutrient solution 5；The effect of battery case 4 is that electricity generation module 3 is carried out Encapsulation and protection, different insulating materials and shape are selected according to different use occasions.
The beneficial effect that the present invention is compared with the prior art is：
1) mechanism that powered fish electroctyte produces electric energy is used for reference, is turned biological energy source using the power generation characteristics of electroctyte Electric energy is changed to, non-pollution discharge is a kind of electrical energy production mode of cleaning.
2) micro-scale of conductive fiber can be customized with macro morphology, and cell can be according to desired trajectory in conductive fiber Upper growth, battery pattern is not limited by arrangement space, it is possible to produce the biobattery of arbitrary shape.
3) biobattery prepared by imitates self power generation fish, adaptable to underwater environment.Available for water of new generation Under mechanically or electrically subcomponent energy resource supply.
4) the active bio battery prepared by is the flexible structure being fabricated by using flexible material, materials for binding biological Degradability, can develop the biobattery for being easy to et al. Ke, the energy resource supply for implants in vivo such as biochips.
Brief description of the drawings
Fig. 1 is biobattery construction method schematic diagram of the invention.
Fig. 2 is biobattery structural representation prepared by the inventive method.
Fig. 3 is ordered nano-fibers prepared by the inventive method.
Fig. 4 is the competent cell fluorogram that the inventive method is printed.
Embodiment
The present invention is described in further detail below in conjunction with the accompanying drawings.
A kind of reference picture 1, active bio battery construction method based on electroctyte, comprises the following steps：
1) powered fish are chosen, separating charged organ extracts the electroctyte inside organ, forms electroctyte suspension Or the mixed solution of electroctyte and hydrogel, electroctyte concentration is 1 × 105To 1 × 107Individual/ml, beats as electroctyte The material of print/extrusion molding；
2) biomaterial of micro-nano fiber manufacture and the mixed solution of conductive material, first configuration quality fraction are configured to For 1% to 15% biomaterial solution, then adding conductive material makes the concentration of conductive material be 10mg/ml to 100mg/ Ml, uniform biological conductive Material cladding solution, the printing for conductive-nano-fibers are formed by dissolving or ultrasonic disperse；
3) biological conductive Material cladding solution is manufactured into using electrostatic spinning or micro-nano 3D printing technique fine with micro-nano The support of structure is tieed up, it is after disinfecting, the mixing of the electroctyte suspension or electroctyte of preparation and hydrogel is molten Liquid is planted or is printed upon on the support with micro-nano fiber structure, obtains the complex structure body of micro-nano structure and cell；
4) complex structure body of micro-nano structure and cell is cultivated in vitro, makes cell according to the direction oriented growth of fiber With propagation, cell tandem is realized；
5) it will realize that the micro-nano structure of cell tandem carries out winding up with the complex structure body of cell or is superimposed, and obtain three-dimensional Active bio battery structure, is then placed in the dynamic biological inside reactor manufactured by insulating materials and is cultivated, and forms stable Electric energy output.
Described step 1) in powered fish be electric eel or electric ray.
Described step 1) in hydrogel be collagen, fibrin or gelatin.
Described step 2) in conductive material include Nano Silver, conducting polymer, CNT, graphene or they Mixture, biomaterial includes polyethylene glycol oxide, polycaprolactone, PLA, chitosan, fibroin albumen or their mixture.
Described step 5) in insulating materials be silicon rubber or lucite.
The described active bio battery construction method based on electroctyte is carried out in an aseptic environment.
As shown in Fig. 2 described active bio battery structure includes electricity generation module 3 and battery case 4, electricity generation module 3 It is made up of electroctyte 1, micro-nano conductive fiber support 2 and cell nutrient solution 5, electroctyte 1 is that biobattery produces electric energy Base unit；It is connected in series or parallel between electroctyte 1 by micro-nano conductive fiber support 2, realizes that stablizing for electric energy is defeated Go out；Electroctyte 1 is survived in cell nutrient solution 5, and the existence of electroctyte 1 needed nutrient matter is provided by cell nutrient solution 5；Electricity The effect of pond shell 4 is that electricity generation module 3 is packaged and protected, and different insulating materials and shape are selected according to different use occasions Shape.
Embodiment 1：A kind of active bio battery construction method based on electroctyte, comprises the following steps：
1) the powered fish such as electric eel, electric ray are chosen, separating charged organ extracts the electroctyte inside organ, forms hair The mixed solution of electric cell and hydrogel, electroctyte concentration is 1 × 105To 1 × 107Individual/ml, is printed as electroctyte The material of type；
2) mixed solution of the biomaterial and conductive material manufactured for micro-nano fiber, first configuration quality point are configured to Number is 2% polyethylene glycol oxide acetum, and adding polycaprolactone powder makes the mass fraction of polycaprolactone be 5%, forms life Thing material solution；Then adding nano-silver thread solution makes the concentration of nano-silver thread be 10mg/mL, and 12 are stirred using magnetic stirring bar After hour, ultrasonic vibration 20 minutes forms uniform biological conductive Material cladding solution；
3) biological conductive Material cladding solution is manufactured into by micro-nano fiber structure using near field electrostatic spinning technology Support, the growth for electroctyte is sought connections with and oriented, after disinfecting, by the electroctyte of preparation and hydrogel Mixed solution is printed upon on the support with micro-nano fiber structure by static dump technique, obtains micro-nano structure and electroctyte Complex structure body；
4) complex structure body of micro-nano structure and cell is cultivated in vitro, makes cell according to the direction oriented growth of fiber With propagation, cell tandem is realized；
5) it will realize that the micro-nano structure of cell tandem carries out winding up with the complex structure body of cell or is superimposed, and obtain three-dimensional Active bio battery structure, is then placed in the dynamic biological inside reactor manufactured by insulating materials and is cultivated, and forms stable Electric energy output.
The present embodiment has the beneficial effect that：Near field electrostatic spinning technology, which can realize that submicron order is other, micro-nano fiber The support printing of structure, by increasing the measures such as moveable platform, modulation process parameter, can be achieved have micro-nano fiber structure Support macrostructure and micro-scale regulation and control；Static dump technique can realize electroctyte with there is micro-nano fiber structure Support on be accurately positioned, electroctyte can along with have micro-nano fiber structure support grow, formed electroctyte with The complex structure body of conductive fiber.Sub-micron manufactured in the present embodiment has the support of micro-nano fiber structure as shown in figure 3, living Orderly distribution of the property cell on the support for having micro-nano fiber structure is as shown in Figure 4.
Embodiment 2：A kind of active bio battery construction method based on electroctyte, comprises the following steps：
1) powered fish are chosen, separating charged organ extracts the electroctyte inside organ, forms electroctyte and water-setting The mixed solution of glue, electroctyte concentration is 1 × 105To 1 × 107Individual/ml, is used as the material of electroctyte extrusion molding；
2) biomaterial of micro-nano fiber manufacture and the mixed solution of conductive material are configured to, first configuration quality fraction is 4% polyethylene glycol oxide acetum, adding polycaprolactone powder makes the mass fraction of polycaprolactone be 6%, forms biological material Expect solution；Then adding nano-silver thread solution makes the concentration of nano-silver thread be 60mg/mL, is stirred 12 hours using magnetic stirring bar Afterwards, ultrasonic vibration 20 minutes, form uniform biological conductive Material cladding solution；
3) biological conductive Material cladding solution is manufactured into by the support with micro-nano fiber structure using extruding-out process, Growth for electroctyte is sought connections with and oriented, after disinfecting, by step 1) electroctyte and hydrogel that prepare Mixed solution is printed upon on the support with micro-nano fiber structure with the mode of extrusion molding, obtains micro-nano structure and electroctyte Complex structure body；
4) complex structure body of micro-nano structure and cell is cultivated in vitro, makes cell according to the direction oriented growth of fiber With propagation, cell tandem is realized；
5) it will realize that the micro-nano structure of cell tandem carries out winding up with the complex structure body of cell or is superimposed, and obtain three-dimensional Active bio battery structure, is then placed in the dynamic biological inside reactor manufactured by insulating materials and is cultivated, and forms stable Electric energy output.
The present embodiment has the beneficial effect that：Extruding-out process efficiency is higher, can realize yardstick it is larger have micro-nano It is prepared by the support of fibre structure；Electroctyte can along with have micro-nano fiber structure support grow, formed electroctyte with The complex structure body of conductive fiber.
Embodiment 3：A kind of active bio battery construction method based on electroctyte, comprises the following steps：
1) the powered fish such as electric eel, electric ray are chosen, separating charged organ extracts the electroctyte inside organ, forms hair The mixed solution of electric cell and hydrogel, electroctyte concentration is 1 × 105To 1 × 107Individual/ml, the plantation for electroctyte；
2) biomaterial of micro-nano fiber manufacture and the mixed solution of conductive material are configured to, first configuration quality fraction is 6% polyethylene glycol oxide acetum, adding polycaprolactone powder makes the mass fraction of polycaprolactone be 8%, forms biological material Expect solution；Then adding nano-silver thread solution makes the concentration of nano-silver thread be 100mg/mL, small using magnetic stirring bar stirring 12 Shi Hou, ultrasonic vibration 20 minutes forms uniform biological conductive Material cladding solution；
3) biological conductive Material cladding solution is manufactured into by the support with micro-nano fiber structure using extruding-out process, Growth for electroctyte is sought connections with and oriented, after disinfecting, by step 1) electroctyte and hydrogel that prepare Mixed solution is planted on the support with micro-nano fiber structure, obtains the complex structure body of micro-nano structure and electroctyte；
4) complex structure body of micro-nano structure and cell is cultivated in vitro, makes cell according to the direction oriented growth of fiber With propagation, cell tandem is realized；
5) it will realize that the micro-nano structure of cell tandem carries out winding up with the complex structure body of cell or is superimposed, and obtain three-dimensional Active bio battery structure, is then placed in the dynamic biological inside reactor manufactured by insulating materials and is cultivated, and forms stable Electric energy output.
The present embodiment has the beneficial effect that：Extruding-out process efficiency is higher, can realize yardstick it is larger have micro-nano fine It is prepared by the support for tieing up structure；Electroctyte is grown along the support with micro-nano fiber structure by the way of plantation, generate electricity Cell survival rate is high, and larger electric energy output can be achieved.

Claims (10)

1. a kind of active bio battery construction method based on electroctyte, it is characterised in that comprise the following steps：
1) powered fish are chosen, separating charged organ extracts the electroctyte inside organ, forms electroctyte suspension or hair The mixed solution of electric cell and hydrogel, electroctyte concentration is 1 × 105To 1 × 107Individual/ml, prints/squeezes as electroctyte Go out the material of shaping；
2) biomaterial of micro-nano fiber manufacture and the mixed solution of conductive material are configured to, configuration quality fraction first is 1% to 15% biomaterial solution, then adding conductive material makes the concentration of conductive material be 10mg/ml to 100mg/ml, Uniform biological conductive Material cladding solution, the printing for conductive-nano-fibers are formed by dissolving or ultrasonic disperse；
3) biological conductive Material cladding solution is manufactured into micro-nano fiber knot using electrostatic spinning or micro-nano 3D printing technique The support of structure, the growth for electroctyte is sought connections with and oriented, after disinfecting, by the electroctyte suspension of preparation or Electroctyte and hydrogel mixed solution plantation or be printed upon on the support with micro-nano fiber structure, obtain micro-nano structure with The complex structure body of electroctyte；
4) complex structure body of micro-nano structure and cell is cultivated in vitro, makes cell according to the direction oriented growth of fiber with increasing Grow, realize cell tandem；
5) it will realize that the micro-nano structure of cell tandem carries out winding up with the complex structure body of cell or is superimposed, obtain three-dimensional activity Biobattery structure, is then placed in the dynamic biological inside reactor manufactured by insulating materials and is cultivated, and forms stable electricity Can output.
2. a kind of active bio battery construction method based on electroctyte according to claim 1, it is characterised in that：Institute The step 1 stated) in powered fish be electric eel or electric ray.
3. a kind of active bio battery construction method based on electroctyte according to claim 1, it is characterised in that：Institute The step 1 stated) in hydrogel be collagen, fibrin or gelatin.
4. a kind of active bio battery construction method based on electroctyte according to claim 1, it is characterised in that：Institute The step 2 stated) in conductive material include Nano Silver, conducting polymer, CNT, graphene or their mixture, it is raw Thing material includes polyethylene glycol oxide, polycaprolactone, PLA, chitosan, fibroin albumen or their mixture.
5. a kind of active bio battery construction method based on electroctyte according to claim 1, it is characterised in that：Institute The step 5 stated) in insulating materials be silicon rubber or lucite.
6. a kind of active bio battery construction method based on electroctyte according to claim 1, it is characterised in that：Institute The active bio battery construction method based on electroctyte stated is carried out in an aseptic environment.
7. a kind of active bio battery construction method based on electroctyte according to claim 1, it is characterised in that：Institute The active bio battery structure stated includes electricity generation module (3) and battery case (4), and electricity generation module (3) is by electroctyte (1), micro- Receive conductive fiber (2) and cell nutrient solution (5) composition, electroctyte (1) be biobattery produce electric energy base unit；Generate electricity It is connected in series or parallel between cell (1) by micro-nano conductive fiber (2)；Electroctyte (1) is survived in cell nutrient solution (5) Interior, battery case (4) effect is that electricity generation module (3) is packaged and protected, according to different use occasions from different exhausted Edge material and shape.
8. a kind of active bio battery construction method based on electroctyte according to claim 1, it is characterised in that bag Include following steps：
1) the powered fish such as electric eel, electric ray are chosen, separating charged organ extracts the electroctyte inside organ, is formed and generates electricity thin Born of the same parents and the mixed solution of hydrogel, electroctyte concentration are 1 × 105To 1 × 107Individual/ml, it is conductive in micro-nano for electroctyte Printing on fibrous framework；
2) biomaterial of micro-nano fiber manufacture and the mixed solution of conductive material are configured to, first configuration quality fraction is 2% Polyethylene glycol oxide acetum, add polycaprolactone powder make polycaprolactone mass fraction be 5%, formed biomaterial it is molten Liquid；Then adding nano-silver thread solution makes the concentration of nano-silver thread be 10mg/mL, after being stirred 12 hours using magnetic stirring bar, Ultrasonic vibration 20 minutes, forms uniform biological conductive Material cladding solution；
3) biological conductive Material cladding solution is manufactured into the branch of micro-nano fiber structure using near field electrostatic spinning technology Frame, the growth for electroctyte is sought connections with and oriented, after disinfecting, by the mixing of the electroctyte of preparation and hydrogel Solution is printed upon on the support with micro-nano fiber structure by static dump technique, obtains answering for micro-nano structure and electroctyte Close structure；
4) complex structure body of micro-nano structure and cell is cultivated in vitro, makes cell according to the direction oriented growth of fiber with increasing Grow, realize cell tandem；
5) it will realize that the micro-nano structure of cell tandem carries out winding up with the complex structure body of cell or is superimposed, obtain three-dimensional activity Biobattery structure, is then placed in the dynamic biological inside reactor manufactured by insulating materials and is cultivated, and forms stable electricity Can output.
9. a kind of active bio battery construction method based on electroctyte according to claim 1, it is characterised in that bag Include following steps：
1) electroctyte inside the powered fish of selection, separating charged organ, extraction organ, formation electroctyte and hydrogel Mixed solution, electroctyte concentration is 1 × 105To 1 × 107Individual/ml, is used as the material of electroctyte extrusion molding；
2) biomaterial of micro-nano fiber manufacture and the mixed solution of conductive material are configured to, first configuration quality fraction is 4% Polyethylene glycol oxide acetum, add polycaprolactone powder make polycaprolactone mass fraction be 6%, formed biomaterial it is molten Liquid；Then adding nano-silver thread solution makes the concentration of nano-silver thread be 60mg/mL, after being stirred 12 hours using magnetic stirring bar, Ultrasonic vibration 20 minutes, forms uniform biological conductive Material cladding solution；
3) biological conductive Material cladding solution is manufactured into by the support with micro-nano fiber structure using extruding-out process, be used for The growth of electroctyte is sought connections with and oriented, after disinfecting, by step 1) prepare electroctyte and hydrogel mixing Solution is printed upon on the support with micro-nano fiber structure with the mode of extrusion molding, obtains answering for micro-nano structure and electroctyte Close structure；
4) complex structure body of micro-nano structure and cell is cultivated in vitro, makes cell according to the direction oriented growth of fiber with increasing Grow, realize cell tandem；
5) it will realize that the micro-nano structure of cell tandem carries out winding up with the complex structure body of cell or is superimposed, obtain three-dimensional activity Biobattery structure, is then placed in the dynamic biological inside reactor manufactured by insulating materials and is cultivated, and forms stable electricity Can output.
10. a kind of active bio battery construction method based on electroctyte according to claim 1, it is characterised in that Comprise the following steps：
1) the powered fish such as electric eel, electric ray are chosen, separating charged organ extracts the electroctyte inside organ, is formed and generates electricity thin Born of the same parents and the mixed solution of hydrogel, electroctyte concentration are 1 × 105To 1 × 107Individual/ml, it is conductive in micro-nano for electroctyte Plantation on fibrous framework；
2) biomaterial of micro-nano fiber manufacture and the mixed solution of conductive material are configured to, first configuration quality fraction is 6% Polyethylene glycol oxide acetum, add polycaprolactone powder make polycaprolactone mass fraction be 8%, formed biomaterial it is molten Liquid；Then adding nano-silver thread solution makes the concentration of nano-silver thread be 100mg/mL, after being stirred 12 hours using magnetic stirring bar, Ultrasonic vibration 20 minutes, forms uniform biological conductive Material cladding solution；
3) biological conductive Material cladding solution is manufactured into by the support with micro-nano fiber structure using extruding-out process, be used for The growth of electroctyte is sought connections with and oriented, after disinfecting, by step 1) prepare electroctyte and hydrogel mixing Solution is planted on the support with micro-nano fiber structure, obtains the complex structure body of micro-nano structure and electroctyte；
4) complex structure body of micro-nano structure and cell is cultivated in vitro, makes cell according to the direction oriented growth of fiber with increasing Grow, realize cell tandem；
5) it will realize that the micro-nano structure of cell tandem carries out winding up with the complex structure body of cell or is superimposed, obtain three-dimensional activity Biobattery structure, is then placed in the dynamic biological inside reactor manufactured by insulating materials and is cultivated, and forms stable electricity Can output.
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110448721A (en) * 2019-07-18 2019-11-15 西安交通大学 A kind of conductive oxidation resistant injectable composite hydrogel and its preparation method and application that stops blooding of antibacterial adherency
DE102018123610A1 (en) * 2018-09-25 2020-03-26 Jochen Schleef Energy storage device for storing electrical energy with at least one storage element

Citations (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5187026A (en) * 1990-10-09 1993-02-16 Scrivano Thomas J Battery adapter
WO2001075989A2 (en) * 2000-04-04 2001-10-11 Moltech Power Systems, Inc. Extended life battery pack with active cooling
US20030219806A1 (en) * 2000-02-22 2003-11-27 Millennium Pharmaceuticals, Inc. Novel 18607, 15603, 69318, 12303, 48000, 52920, 5433, 38554, 57301, 58324, 55063, 52991, 59914, 59921 and 33751 molecules and uses therefor
US20040048150A1 (en) * 2002-09-06 2004-03-11 Crouch Dell Albert Battery cell having edge support and method of making the same
US20050215764A1 (en) * 2004-03-24 2005-09-29 Tuszynski Jack A Biological polymer with differently charged portions
CN101081313A (en) * 2007-06-19 2007-12-05 东南大学 Bionics artificial thews material based on electro spinning superfine fibre and method for making same
CN101635532A (en) * 2009-08-25 2010-01-27 北京大学 Method for converting free energy stored in ion concentration gradient form into electric energy
CN102198022A (en) * 2011-05-23 2011-09-28 西安交通大学 Solid forming method of active cell-hydrogel organ structure
CN102514697A (en) * 2011-12-20 2012-06-27 南京航空航天大学 Bionic robot stingray and movement method thereof
CN103976805A (en) * 2014-05-29 2014-08-13 西安交通大学 Fabrication method for hydrogel/macromolecule polymer film muscular tissue support
WO2015015540A1 (en) * 2013-07-27 2015-02-05 エクセルギー・パワー・システムズ株式会社 Negative electrode material for battery, negative electrode for battery, and battery
CN104645412A (en) * 2015-01-28 2015-05-27 南方医科大学 Preparation method of bionic artificial nerve scaffold established by collagen
CN105428090A (en) * 2015-12-13 2016-03-23 复旦大学 Fibrous super capacitor possessing high output voltage and manufacturing method thereof
CN105688281A (en) * 2016-03-08 2016-06-22 北京航空航天大学 Vascularized artificial tissue organ construction method combining cell three-dimensional printing and dielectrophoresis adsorption
CN106222085A (en) * 2016-07-28 2016-12-14 西安交通大学 A kind of high-precision biology is combined 3D printing equipment and Method of printing
WO2018009032A1 (en) * 2016-07-07 2018-01-11 인하대학교 산학협력단 Biologically extracted melanin/polymer composite having high electrical conductivity and dense structure, and preparation method therefor
JP2018110098A (en) * 2016-12-28 2018-07-12 パナソニックＩｐマネジメント株式会社 battery
CN108384742A (en) * 2018-02-11 2018-08-10 大连金玛健康产业发展有限公司 A kind of novel activated dose and preparation method thereof for immune cell expansion
CN109301298A (en) * 2018-08-29 2019-02-01 中南大学 A kind of life battery and preparation method thereof and the device for being used to prepare life battery
CN109436321A (en) * 2018-10-25 2019-03-08 南京理工大学 A kind of water sky of bionical electric ray is dual-purpose to detect dozen type unmanned plane
US10737093B2 (en) * 2014-07-03 2020-08-11 The Vitasken Venture Club Wearable item for increased application of nutrients

Patent Citations (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5187026A (en) * 1990-10-09 1993-02-16 Scrivano Thomas J Battery adapter
US20030219806A1 (en) * 2000-02-22 2003-11-27 Millennium Pharmaceuticals, Inc. Novel 18607, 15603, 69318, 12303, 48000, 52920, 5433, 38554, 57301, 58324, 55063, 52991, 59914, 59921 and 33751 molecules and uses therefor
WO2001075989A2 (en) * 2000-04-04 2001-10-11 Moltech Power Systems, Inc. Extended life battery pack with active cooling
US20040048150A1 (en) * 2002-09-06 2004-03-11 Crouch Dell Albert Battery cell having edge support and method of making the same
US20050215764A1 (en) * 2004-03-24 2005-09-29 Tuszynski Jack A Biological polymer with differently charged portions
CN101081313A (en) * 2007-06-19 2007-12-05 东南大学 Bionics artificial thews material based on electro spinning superfine fibre and method for making same
CN101635532A (en) * 2009-08-25 2010-01-27 北京大学 Method for converting free energy stored in ion concentration gradient form into electric energy
CN102198022A (en) * 2011-05-23 2011-09-28 西安交通大学 Solid forming method of active cell-hydrogel organ structure
CN102514697A (en) * 2011-12-20 2012-06-27 南京航空航天大学 Bionic robot stingray and movement method thereof
WO2015015540A1 (en) * 2013-07-27 2015-02-05 エクセルギー・パワー・システムズ株式会社 Negative electrode material for battery, negative electrode for battery, and battery
CN103976805A (en) * 2014-05-29 2014-08-13 西安交通大学 Fabrication method for hydrogel/macromolecule polymer film muscular tissue support
US10737093B2 (en) * 2014-07-03 2020-08-11 The Vitasken Venture Club Wearable item for increased application of nutrients
CN104645412A (en) * 2015-01-28 2015-05-27 南方医科大学 Preparation method of bionic artificial nerve scaffold established by collagen
CN105428090A (en) * 2015-12-13 2016-03-23 复旦大学 Fibrous super capacitor possessing high output voltage and manufacturing method thereof
CN105688281A (en) * 2016-03-08 2016-06-22 北京航空航天大学 Vascularized artificial tissue organ construction method combining cell three-dimensional printing and dielectrophoresis adsorption
WO2018009032A1 (en) * 2016-07-07 2018-01-11 인하대학교 산학협력단 Biologically extracted melanin/polymer composite having high electrical conductivity and dense structure, and preparation method therefor
CN106222085A (en) * 2016-07-28 2016-12-14 西安交通大学 A kind of high-precision biology is combined 3D printing equipment and Method of printing
JP2018110098A (en) * 2016-12-28 2018-07-12 パナソニックＩｐマネジメント株式会社 battery
CN108384742A (en) * 2018-02-11 2018-08-10 大连金玛健康产业发展有限公司 A kind of novel activated dose and preparation method thereof for immune cell expansion
CN109301298A (en) * 2018-08-29 2019-02-01 中南大学 A kind of life battery and preparation method thereof and the device for being used to prepare life battery
CN109436321A (en) * 2018-10-25 2019-03-08 南京理工大学 A kind of water sky of bionical electric ray is dual-purpose to detect dozen type unmanned plane

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
HANGNILO ROBERT: ""Charging a Battery with the Electric Organ Discharge of Malapterurus Electricus Catfish"", 《INTERNATIONAL JOURNAL OF MODERN ENGINEERING RESEARCH (IJMER》 *
HAO SUN等: ""Electrochemical Capacitors with High Output Voltages that Mimic Electric Eels"", 《ADVANCED MATERIALS》 *
JIAN XU等: ""Sythetic protocells to mimic and test cell function"", 《ADVANCED MATERIALS》 *
JIANKANG HE等: ""Electrohydrodynamic 3D printing of microscale poly (ε-caprolactone) scaffolds with multi-walled carbon nanotubes"", 《BIOFABRICATION》 *
THOMAS B. H. SCHROEDER等: ""An electric-eel-inspired soft power source from stacked hydrogels"", 《NATURE》 *
YANG JIE等: ""A Structural Bionic Design: From Electric Organs to Systematic Triboelectric Generators"", 《NANO ENERGY》 *
刘玉宝: ""生物电"", 《学科教育》 *
叶澍: ""鱼类为什么会放电"", 《海洋世界》 *
张晓燕编著: "《无尽的海洋世界》", 31 March 2014, 吉林出版集团有限责任公司 *
温海深主编: "《水产动物生理学》", 31 March 2009, 中国海洋大学出版社 *
知乎: ""有没有可能通过电鳗的发电原理，人工培养电细胞并收集电能，进行利用"", 《知乎》 *
贺佩等: ""多材料打印仿生离子凝胶电池研究"", 《机械工程学报》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102018123610A1 (en) * 2018-09-25 2020-03-26 Jochen Schleef Energy storage device for storing electrical energy with at least one storage element
CN110448721A (en) * 2019-07-18 2019-11-15 西安交通大学 A kind of conductive oxidation resistant injectable composite hydrogel and its preparation method and application that stops blooding of antibacterial adherency

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