CN107151218A - A kind of method that dibutyl phthalate is extracted in the ballstone algae from ocean - Google Patents
A kind of method that dibutyl phthalate is extracted in the ballstone algae from ocean Download PDFInfo
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- CN107151218A CN107151218A CN201710367720.5A CN201710367720A CN107151218A CN 107151218 A CN107151218 A CN 107151218A CN 201710367720 A CN201710367720 A CN 201710367720A CN 107151218 A CN107151218 A CN 107151218A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/56—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses in a kind of ballstone algae from ocean extract dibutyl phthalate method, successively with：Extracted with ethyl acetate；Chromatographed with reverse phase silica gel post；Chromatographed with sephadex column Sephadex LH 20；Chromatographed with reverse phase silica gel post；Chromatographed with normal phase silicagel column；The method prepared with PTLC extracts dibutyl phthalate from the ballstone algae of ocean.The present invention has carried out separation, purifying to the dibutyl phthalate of ocean ballstone algae ethyl acetate phase extract first, and the antibacterial activity of dibutyl phthalate is studied, to be laid the foundation for follow-up large-scale production dibutyl phthalate.
Description
Technical field
The present invention relates to the technical field of biological agent, more particularly to one kind phthalic acid is extracted from the ballstone algae of ocean
The method of dibutyl ester.
Background technology
Ocean ballstone algae (Coccolithophores) is a miniature phytoplankton of class single-cell sea, is under the jurisdiction of and determines whip algae
Door (Haptophyta=Prymnesiophyta) Haptophyceae (Prymnesiophyceae).In the ballstone algae metabolite of ocean
Rich in various bioactivators, such as docosahexaenoic acid (DHA), polyketides, especially marine microalgae cell
Middle total lipid content reaches as high as the 50% of dry cell weight, and these compounds have the biologies such as well antibacterial, anti-oxidant, antitumor
Function, has broad application prospects in biological technical field.But at present about ocean ballstone algae lipid active material field
Research is less.
In view of this, dibutyl phthalate is extracted during the present inventor studies and devises a kind of ballstone algae from ocean
Thus method, this case produces.
The content of the invention
It is an object of the invention to provide a kind of method that dibutyl phthalate is extracted in ballstone algae from ocean, it is intended to
By methods such as TLC, silica gel column chromatography, gel filtration chromatography method, preparative thin layer PTLC to ocean ballstone algae acetic acid
The dibutyl phthalate of ethyl ester phase extract is separated, purified, in order to follow-up large-scale production phthalic acid
Dibutyl ester.
In order to solve above-mentioned purpose, the technical solution adopted by the present invention is as follows：
In a kind of ballstone algae from ocean extract dibutyl phthalate method, successively with：Extracted with ethyl acetate
Take；Chromatographed with reverse phase silica gel post；Chromatographed with sephadex column Sephadex LH-20；Carried out with reverse phase silica gel post
Chromatography；Chromatographed with normal phase silicagel column；The method prepared with PTLC extracts dibutyl phthalate from the ballstone algae of ocean.
As the preferred embodiment of embodiment, the method for said extracted dibutyl phthalate specifically includes following steps：
Step 1: preparing ocean ballstone algae ethyl acetate phase extract：First extracted with ethanol solution, obtain ethanol extract,
Extraction processing is carried out by extractant of ethyl acetate again, combining extraction liquid is concentrated under reduced pressure to give ocean ballstone algae ethyl acetate phase
Extract；
As the preferred embodiment of embodiment, ocean ballstone algae grows to stationary phase, and harvest, freeze-drying obtains ocean ballstone
Algae powder g, is extracted for 75% ethanol solution with mass fraction, obtains ethanol extract, add isometric ethyl acetate and water,
Extract repeatedly, collect ethyl acetate phase, merge, be concentrated under reduced pressure into dry, acquisition ocean ballstone algae ethyl acetate phase extract；
Step 2: reversed-phase silica gel column chromatography prepares crude product of dibutyl phthalate：By the ocean ballstone algae of acquisition
Ethyl acetate phase extract carries out Image processing with reverse phase silica gel post, collects, and detects, merges, being concentrated under reduced pressure into dry, obtaining adjacent benzene
Crude product of dibutyl carboxylic acid；
It is as the preferred embodiment of embodiment, ocean ballstone algae ethyl acetate phase extract is molten with the appropriate pure methanol of analysis
Solution, addition there just was not extract to be advisable with methanol, carried out reversed-phase silica gel column chromatography；Water is used successively, and mass fraction is 25%
Methanol solution, mass fraction be 50% methanol solution, mass fraction be 75% methanol solution, methanol elution, Mei Geti
Degree elution 1.5L, flow control is 30mL/min, and 1 is collected per 200mL and is managed, and revolving tentatively analyzes effluent with TLC, merges adjacent
Dibatyl phithalate high concentration flow is segmented, and is concentrated under reduced pressure into dry, acquisition crude product of dibutyl phthalate；
Step 3: sephadex column Sephadex LH-20 chromatographies prepare the secondary crude product of dibutyl phthalate：Will
Crude product of dibutyl phthalate carries out Image processing with sephadex column Sephadex LH-20, collects, detection, conjunction
And, be concentrated under reduced pressure into dry, obtain the secondary crude product of dibutyl phthalate；
After crude product proper amount of methanol of dibutyl phthalate is dissolved, upper sephadex column Sephadex LH-
20, eluted with methanol as eluant, eluent, flow control 12-13S/drop collects each cut, often using automatic collector
30min collects one and managed, and tentatively analyzes effluent with TLC, merges the cut of 80 to 90 pipes, is concentrated under reduced pressure into dry, the adjacent benzene two of acquisition
The secondary crude product of formic acid dibutyl ester；
Step 4: reversed-phase silica gel column chromatography prepares fine work of dibutyl phthalate：By the phthalic acid of acquisition
The secondary crude product of dibutyl ester carries out Image processing with reverse phase silica gel post, collects, and detects, merges, being concentrated under reduced pressure into dry, obtaining adjacent benzene two
Fine work of formic acid dibutyl ester；
By the secondary crude product of dibutyl phthalate, mass fraction is used successively for 30%, 40%, 50%, 60%, 70%
Aqueous methanol gradient elution, finally with methanol elution carry out reversed-phase silica gel column chromatography, each gradient elution 300mL, flow velocity
Control as 5mL/min, revolving tentatively analyzes effluent with TLC, merge the segmentation of dibutyl phthalate high concentration flow, decompression
It is concentrated to dryness, obtains fine work of dibutyl phthalate；
Step 5: normal-phase silica gel column chromatography prepares the secondary fine work of dibutyl phthalate：By the phthalic acid of acquisition
Fine work of dibutyl ester carries out Image processing with normal phase silicagel column, collects, and detects, merges, being concentrated under reduced pressure into dry, obtaining adjacent benzene two
The secondary fine work of formic acid dibutyl ester；
Fine work of dibutyl phthalate is passed through into normal-phase silica gel column chromatography：Sample, silica gel oil are mixed with silica white
Fill after post, sample upper prop and to be eluted with petroleum ether acetone system after ether saturation, the volume ratio of petroleum ether and acetone is 200：1,
Cut is collected, dry, the acquisition secondary fine work of dibutyl phthalate is concentrated under reduced pressure into；
Step 6: PTLC prepares dibutyl phthalate sterling：By the secondary fine work of the dibutyl phthalate of acquisition
PTLC is carried out, point sample, expansion, scraper plate, sample reclaim, is stored in -20 DEG C, obtains dibutyl phthalate sterling；
The secondary fine work of dibutyl phthalate is passed through into PTLC, dibutyl phthalate is finally given；Using efficient
Thin layer chromatography board, is petroleum ether in volume ratio：Ethyl acetate=80：Deploy in 1 solvent, blade is used in rear and front end colour developing
Center section is scraped, merges, is dissolved with methanol, high speed centrifugation, supernatant is collected, is concentrated under reduced pressure into dry, finally give adjacent benzene two
Formic acid dibutyl ester.
A kind of dibutyl phthalate is preparing anti-Escherichia coli, bacillus subtilis and staphylococcus aureus medicament
In application.
Because present system employs above-mentioned technical scheme so that this practicality has the advantages that：This hair
It is bright to use the methods such as TLC, silica gel column chromatography, gel filtration chromatography method, preparative thin layer first to ocean ballstone algae second
The dibutyl phthalate of acetoacetic ester phase extract has carried out separation, purifying, and the antibacterial of dibutyl phthalate is lived
Property studied, to be laid the foundation for follow-up large-scale production dibutyl phthalate.
Brief description of the drawings
Fig. 1 is dibutyl phthalate TLC of the present invention figure.
Embodiment
The method that dibutyl phthalate is extracted in a kind of ballstone algae from ocean of embodiment 1
Present invention is disclosed in a kind of ballstone algae from ocean extract dibutyl phthalate method, successively with：Use second
Acetoacetic ester is extracted；Chromatographed with reverse phase silica gel post；Chromatographed with sephadex column Sephadex LH-20；With anti-
Phase silicagel column is chromatographed；Chromatographed with normal phase silicagel column；The method prepared with PTLC extracts adjacent benzene from the ballstone algae of ocean
Dibutyl carboxylic acid.
As the preferred embodiment of embodiment, the method for said extracted dibutyl phthalate specifically includes following steps：
Step 1: preparing ocean ballstone algae ethyl acetate phase extract：First extracted with ethanol solution, obtain ethanol extract,
Extraction processing is carried out by extractant of ethyl acetate again, combining extraction liquid is concentrated under reduced pressure to give ocean ballstone algae ethyl acetate phase
Extract；
Ballstone algae grows to stationary phase for ocean, and harvest, freeze-drying obtains ocean ballstone algae powder 19.3740g, uses quality
Fraction is 75% alcohol steep, obtains ethanol extract 5.1247g, adds isometric ethyl acetate and water, extracts repeatedly, is received
Collect ethyl acetate phase, merge, be concentrated under reduced pressure into dry, acquisition ocean ballstone algae ethyl acetate phase extract 4.2357g；
Step 2: reversed-phase silica gel column chromatography prepares crude product of dibutyl phthalate：By the ocean ballstone algae of acquisition
Ethyl acetate phase extract carries out Image processing with reverse phase silica gel post, collects, and detects, merges, being concentrated under reduced pressure into dry, obtaining adjacent benzene
Crude product of dibutyl carboxylic acid；
By 4.2357g oceans ballstone algae ethyl acetate phase extract with after the appropriate pure methanol dissolving of analysis, carry out anti-phase
Silica gel (180g) column chromatography；Water is used successively, and mass fraction is 25% methanol solution, and mass fraction is 50% methanol solution,
Mass fraction is 75% methanol solution, and methanol is eluted, each gradient elution 1.5L, and flow control is 30mL/min, per 200mL
1 pipe is collected, revolving tentatively analyzes effluent with TLC, merges the segmentation of palmitic acid high concentration flow, be concentrated under reduced pressure into dry, the adjacent benzene of acquisition
Crude product of dibutyl carboxylic acid；
Step 3: sephadex column Sephadex LH-20 chromatographies prepare the secondary crude product of dibutyl phthalate：Will
Crude product of dibutyl phthalate carries out Image processing with sephadex column Sephadex LH-20, collects, detection, conjunction
And, be concentrated under reduced pressure into dry, obtain the secondary crude product of dibutyl phthalate；
After ocean ballstone algae ethyl acetate phase crude product proper amount of methanol of extract is dissolved, upper sephadex column
Sephadex LH-20, are eluted with methanol as eluant, eluent, flow control 12-13S/drop, are received using automatic collector
Collect each cut, per 30min collect one manage, tentatively with TLC analyze effluent, merge 80 to 90 pipes cut, be concentrated under reduced pressure into it is dry,
Obtain the secondary crude product of dibutyl phthalate；
Step 4: reversed-phase silica gel column chromatography prepares fine work of dibutyl phthalate：By the phthalic acid of acquisition
The secondary crude product of dibutyl ester carries out Image processing with reverse phase silica gel post, collects, and detects, merges, being concentrated under reduced pressure into dry, obtaining adjacent benzene two
Fine work of formic acid dibutyl ester；
By the secondary crude product of dibutyl phthalate, mass fraction is used successively for 30%, 40%, 50%, 60%, 70%
Methanol-water gradient elution, finally with methanol elution carry out reversed-phase silica gel column chromatography, each gradient elution 300mL, flow control
For 5mL/min, revolving tentatively analyzes effluent with TLC, merges the segmentation of dibutyl phthalate high concentration flow, is concentrated under reduced pressure
To dry, fine work of dibutyl phthalate is obtained；
Step 5: normal-phase silica gel column chromatography prepares the secondary fine work of dibutyl phthalate：By the phthalic acid of acquisition
Fine work of dibutyl ester carries out Image processing with normal phase silicagel column, collects, and detects, merges, being concentrated under reduced pressure into dry, obtaining adjacent benzene two
The secondary fine work of formic acid dibutyl ester；
Fine work of dibutyl phthalate is passed through into normal-phase silica gel column chromatography, sample, silica gel oil are mixed with silica white
Fill after post, sample upper prop and to be eluted with petroleum ether and acetone system after ether saturation, the volume ratio of petroleum ether and acetone is 200：
1, cut is collected, dry, the acquisition secondary fine work of dibutyl phthalate is concentrated under reduced pressure into；
Step 6: PTLC prepares dibutyl phthalate sterling：By the secondary fine work of the dibutyl phthalate of acquisition
PTLC is carried out, point sample, expansion, scraper plate, sample reclaim, is stored in -20 DEG C, obtains dibutyl phthalate sterling；
The secondary fine work of dibutyl phthalate is passed through into PTLC, dibutyl phthalate is finally given：Using efficient
Thin layer chromatography board, be in volume ratio：Petroleum ether：Ethyl acetate=80：Deploy in 1 solvent, blade is used in rear and front end colour developing
Center section is scraped, merges, is dissolved with methanol, high speed centrifugation, supernatant is collected, is concentrated under reduced pressure into dry, finally give adjacent benzene two
Formic acid dibutyl ester.
The bacteriostatic activity of the dibutyl phthalate of embodiment 2
Minimal inhibitory concentration MIC measure：
Test tube doubling dilution：The decoction of various concentrations is obtained using the method for doubling dilution, inoculation sets sun for examination bacterium
Property control and negative control, be incubated at a suitable temperature, so that it is determined that MIC value.
The bacteriostatic activity of the dibutyl phthalate of table 1
Note:1))-and without inhibition zone ,+7.1~9.0mm, ++ 9.1~11.0mm.
Antibacterial tests result is carried out to dibutyl phthalate to show, dibutyl phthalate is to Escherichia coli, withered
Careless bacillus, staphylococcus aureus have inhibitory action, wherein having stronger to bacillus subtilis, staphylococcus aureus
Inhibitory action.
The dibutyl phthalate of table 2 difference dilution factor MIC measure
Note：- without colony growth ,+there is colony growth
MIC determination experiment results show that dibutyl phthalate has resistance bacillus subtilis, Staphylococcus aureus
The effect of bacterium, its MIC value is respectively 5mg/mL, 10mg/mL.
The cytotoxic activity of embodiment 3 is tested
A. cell cryopreservation：
Prepare 10%DMSO calf serum frozen stock solutions；Take the logarithm the phase growth LO2, Hep G2 cells, disappeared with trypsase
Change monolayer growth cell；1000rpm centrifuges 5min；Supernatant is removed, new frozen stock solution is added；It is sub-packed in cryopreservation tube, has marked name
Title, time；The cryopreservation tube that will be equipped with cell is put into -20 DEG C of refrigerator 2h, is then placed in -70 DEG C of refrigerator overnights, and rapid take out is frozen
Pipe is deposited, moves into and is frozen in liquid nitrogen.
B. cell recovery：
Cell cryopreservation tube is taken out from liquid nitrogen, is thawed rapidly in 37 DEG C of water-baths, this process is controlled within 1 minute；Inhale
Go out cell suspending liquid into centrifuge tube and add 10 times of culture mediums, gently mix；1000rpm centrifuges 5min；Supernatant is outwelled, weight
Once, blood counting chamber is counted for after backwashing, stands the 37 DEG C of cultures of CO2 incubators；Next day changes culture medium and continues to cultivate.
C.MTT methods detect cell viability：
The selected tumour cell of this experiment is liver cancer cell lines Hep G2 and normal liver cell LO2.Logarithm is digested with pancreatin
The tumour cell in growth period, is counted with blood counting chamber after adding fresh medium, adjusts and 96 holes are inoculated in after cell concentration
Plate, is placed in culture 24h in 37 DEG C of CO2 incubators.6 kinds of monomeric compounds are configured to the decoction that concentration is 10mg/mL, Mei Genong
Three parallel laboratory tests are spent, using cis-platinum as positive control.20 μ L MTT solution, 37 DEG C of culture 4h are added in per hole.Supernatant is removed, often
Hole adds the μ L of DMSO 150.37 DEG C of shaking table cultures 2 hours, determine optical density (OD490) value at ELIASA 490nm, calculate suppression
Rate processed.
Inhibiting rate %=(1- experimental groups mean OD value/control group mean OD value) × 100%
The dibutyl phthalate of table 3 is to LO2 (normal) and the inhibiting rate of Hep G2 (liver cancer) cell
Cytotoxic activity experiment shows that dibutyl phthalate has certain inhibitory action to Hep G2 liver cancer cells,
But DeGrain, while it also has certain detrimental effect to normal cell LO2.
It is the colourless transparent oil liquid for having fragranced that n-butyl phthalate, which is also known as DBP, be widely present in cigarette,
In flue gas, mainly it is used as plasticiser in food and industry, it is also possible to make external insecticide.This result of study shows O-phthalic
Dibutyl phthalate DBP is respectively 5mg/mL, 10mg/mL, this result to the MIC value of bacillus subtilis, staphylococcus aureus
To control this important sea-farming disease of bacillus subtilis, staphylococcus aureus using dibutyl phthalate in the future
Evil problem provides a new thread.Other dibutyl phthalate also has certain cytotoxic activity, in this experiment
Liver cancer cells Hep G2 have resistance effect.
It is described above, only present pre-ferred embodiments, therefore the scope that the present invention is implemented can not be limited according to this, i.e., according to
The equivalent changes and modifications that the scope of the claims of the present invention and description are made, all should still belong in the range of the present invention covers.
Claims (9)
1. the method for dibutyl phthalate is extracted in a kind of ballstone algae from ocean, it is characterised in that：Successively with：Use acetic acid second
Ester is extracted；Chromatographed with reverse phase silica gel post；Chromatographed with sephadex column Sephadex LH-20；Use anti-phase silicon
Glue post is chromatographed；Chromatographed with normal phase silicagel column；The method prepared with PTLC extracts O-phthalic from the ballstone algae of ocean
Dibutyl phthalate.
2. a kind of method as claimed in claim 1 that dibutyl phthalate is extracted from the ballstone algae of ocean, its feature exists
In：Comprise the following steps：
Step 1: preparing ocean ballstone algae ethyl acetate phase extract：First extracted with ethanol solution, acquisition ethanol extract, then with
Ethyl acetate is that extractant carries out extraction processing, and combining extraction liquid is concentrated under reduced pressure to give ocean ballstone algae ethyl acetate phase extraction
Thing；
Step 2: reversed-phase silica gel column chromatography prepares crude product of dibutyl phthalate：By the ocean ballstone algae acetic acid of acquisition
Ethyl ester phase extract carries out Image processing with reverse phase silica gel post, collects, and detects, merges, being concentrated under reduced pressure into dry, obtaining O-phthalic
Crude product of dibutyl phthalate；
Step 3: sephadex column Sephadex LH-20 chromatographies prepare the secondary crude product of dibutyl phthalate：By adjacent benzene
Crude product of dibutyl carboxylic acid carries out Image processing with sephadex column Sephadex LH-20, collects, detect, merge,
It is concentrated under reduced pressure into dry, obtains the secondary crude product of dibutyl phthalate；
Step 4: reversed-phase silica gel column chromatography prepares fine work of dibutyl phthalate：By the fourth of phthalic acid two of acquisition
The secondary crude product of ester carries out Image processing with reverse phase silica gel post, collects, and detects, merges, being concentrated under reduced pressure into dry, obtaining phthalic acid
Fine work of dibutyl ester；
Step 5: normal-phase silica gel column chromatography prepares the secondary fine work of dibutyl phthalate：By the fourth of phthalic acid two of acquisition
Fine work of ester carries out Image processing with normal phase silicagel column, collects, and detects, merges, being concentrated under reduced pressure into dry, obtaining phthalic acid
The secondary fine work of dibutyl ester；
Step 6: PTLC prepares dibutyl phthalate sterling：The secondary fine work of the dibutyl phthalate of acquisition is carried out
PTLC, point sample, expansion, scraper plate, sample is reclaimed, and is stored in -20 DEG C, is obtained dibutyl phthalate sterling.
3. extracting the method for dibutyl phthalate in a kind of ballstone algae from ocean as claimed in claim 2, its feature exists
In：The specific method of the step one is：Ballstone algae grows to stationary phase for ocean, and harvest, freeze-drying obtains ocean ballstone algae
Algae powder g, is extracted for 75% ethanol solution with mass fraction, obtains ethanol extract, add isometric ethyl acetate and water, instead
Multiple extraction, collects ethyl acetate phase, merges, is concentrated under reduced pressure into dry, acquisition ocean ballstone algae ethyl acetate phase extract.
4. extracting the method for dibutyl phthalate in a kind of ballstone algae from ocean as claimed in claim 2, its feature exists
In：The specific method of the step 2 is：Ocean ballstone algae ethyl acetate phase extract is dissolved with the appropriate pure methanol of analysis,
Addition there just was not extract to be advisable with methanol, carried out reversed-phase silica gel column chromatography；Water is used successively, and mass fraction is 25% first
Alcoholic solution, mass fraction is 50% methanol solution, and mass fraction is 75% methanol solution, methanol elution, each gradient elution
1.5 L, flow control is 30 mL/min, and every 200 mL collects 1 and managed, and revolving tentatively analyzes effluent with TLC, merges adjacent benzene two
Formic acid dibutyl ester high concentration flow is segmented, and is concentrated under reduced pressure into dry, acquisition crude product of dibutyl phthalate.
5. extracting the method for dibutyl phthalate in a kind of ballstone algae from ocean as claimed in claim 2, its feature exists
In：The specific method of the step 3 is：After crude product proper amount of methanol of dibutyl phthalate is dissolved, upper glucan
Gel column Sephadex LH-20, are eluted with methanol as eluant, eluent, flow control 12-13 S/drop, use automatic receipts
Storage collects each cut, and one is collected per 30min and is managed, tentatively effluent is analyzed with TLC, merges the cut of 80 to 90 pipes, is depressurized dense
It is reduced to dry, the acquisition secondary crude product of dibutyl phthalate.
6. extracting the method for dibutyl phthalate in a kind of ballstone algae from ocean as claimed in claim 2, its feature exists
In：The specific method of the step 4 is：By the secondary crude product of dibutyl phthalate, mass fraction is used successively for 30%,
40%, 50%, 60%, 70% aqueous methanol gradient elution finally carries out reversed-phase silica gel column chromatography, each gradient with methanol elution
300 mL are eluted, flow control is 5 mL/min, and revolving tentatively analyzes effluent with TLC, merges dibutyl phthalate high
Concentration flow point section, is concentrated under reduced pressure into dry, acquisition fine work of dibutyl phthalate.
7. extracting the method for dibutyl phthalate in a kind of ballstone algae from ocean as claimed in claim 2, its feature exists
In：The specific method of the step 5 is：Fine work of dibutyl phthalate is passed through into normal-phase silica gel column chromatography：Use silica gel
Powder mixes sample, and silica gel is eluted with petroleum ether acetone system with being filled after petroleum ether saturation after post, sample upper prop, petroleum ether and acetone
Volume ratio be 200：1, cut is collected, dry, the acquisition secondary fine work of dibutyl phthalate is concentrated under reduced pressure into.
8. extracting the method for dibutyl phthalate in a kind of ballstone algae from ocean as claimed in claim 2, its feature exists
In：The specific method of the step 6 is：The secondary fine work of dibutyl phthalate is passed through into PTLC, O-phthalic is finally given
Dibutyl phthalate；It is petroleum ether in volume ratio using High Performance Thin Layer Chromatography plate：Ethyl acetate=80：Deploy in 1 solvent, it is front and rear
Two ends develop the color, with blade scrape center section, merge, dissolved with methanol, high speed centrifugation, collect supernatant, be concentrated under reduced pressure into it is dry,
Finally give dibutyl phthalate.
9. a kind of dibutyl phthalate is in anti-Escherichia coli, bacillus subtilis and staphylococcus aureus medicament is prepared
Application.
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| CN107141198A (en) * | 2017-05-23 | 2017-09-08 | 集美大学 | A kind of method that phytol is extracted in the ballstone algae from ocean |
| CN107141198B (en) * | 2017-05-23 | 2020-11-06 | 集美大学 | Method for extracting phytol from marine chlorella |
| CN108017554A (en) * | 2018-01-17 | 2018-05-11 | 集美大学 | A kind of method that ceramide is extracted in the ballstone algae from ocean |
| CN108017554B (en) * | 2018-01-17 | 2020-10-02 | 集美大学 | Method for extracting ceramide from marine coccolithosiphon |
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