CN107144453A - Amino acid and the dry blood cake quality-control product of fatty acyl carnitine and preparation method thereof - Google Patents
Amino acid and the dry blood cake quality-control product of fatty acyl carnitine and preparation method thereof Download PDFInfo
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Abstract
The invention discloses amino acid and the dry blood cake quality-control product of fatty acyl carnitine and preparation method thereof, dry blood cake quality-control product is made up of amino acid standard items, fatty acyl carnitine standard items, red blood cell, and amino acid standard items have 8 kinds, and fatty acyl carnitine standard items have 15 kinds.The use of standard items is greatly reduced with the dry blood cake quality-control product of fatty acyl carnitine for the amino acid of the present invention, and stability, homogeneity all meet the requirements, and amino acid can be preserved more than 4 months with the dry blood cake Quality Control of fatty acyl carnitine under conditions of 20 DEG C.Preparation method cost is low, simple to operate, larger amount of can be prepared, usage cycles are longer, is not required to frequently change Quality Control lot number, facilitates daily Analysis of quality control, so as to meet demand of the clinical and routine testing to quality-control product.
Description
Technical field
The present invention relates to a kind of detection quality-control product and its compound method, more particularly to amino acid and the dry blood cake of fatty acyl carnitine
Quality-control product and preparation method thereof.
Background technology
Inherited Metabolic Disorders refer to because gene mutation makes the protein function defects such as the enzyme, acceptor, carrier of synthesis, cause in vivo
There are various exceptions in terms of synthesis, metabolism, transhipment and storage in biochemical substances.It belongs to a part for single gene inheritance disease,
Overwhelming majority category autosomal recessive inheritance, it is a small number of to be lost for autosomal dominant inheritance, the chain sex-linked inheritance of X, or mitochondria
Pass；Inherited Metabolic Disorders disease is various, common 500-600 kinds, and sum may be up to thousands of remaining kinds；Single disease incidence of disease is low, but overall hair
Sick rate is higher, has been reported that illness rate more than 0.5%.
The examination of newborn infant diseases is the prevention effective three stage mitigation of inborn defect, to improving the overall quality of newborns
Have great significance.The Inherited Metabolic Disorders project of tandem mass spectrum detection can detect 18 kinds of amino acid and 45 kinds of acyls in 2 minutes
Base carnitine, above-mentioned Inherited Metabolic Disorders can detect more than 45 kinds Inherited Metabolic Disorders, and various high-end laboratories are widely used at present,
Can more effectively, detect inherited metabolic disorder earlier, provide effective foundation for immunotherapy targeted autoantibody.With the technology increasingly
Clinical auxiliary diagnosis is applied to more, and the accuracy that vivo acid and fatty acyl carnitine are determined seems more and more important.
Quality-control product is used exclusively for indoor quality control, stable, approximate with testing sample material, is to ensure accurate
The important foundation of measure, and a permanently effective monitoring can be carried out to method stability, with tandem mass spectrum detection increasingly
Clinical auxiliary diagnosis is applied to more, and the demand of related quality-control product also gradually increases.
Because the classes of compounds of tandem mass spectrum one-time detection is various, it is however generally that, to ensure its Quality Control effect, its Quality Control
It is also required to add various ingredients in product, and because the characteristic of each component is different, it is difficult to prepare the quality-control product of stable homogeneous.Cause
This, at present, commercialization amino acid and the dry blood cake quality-control product source of fatty acyl carnitine are few, and valuable product, and the arrival cycle is long, together
One lot number no stability is originated.Therefore, research and development stability, all satisfactory quality-control product preparation method of homogeneity have important meaning
Justice.
The content of the invention
It is an object of the invention to provide a kind of composition is relatively easy, stability, the satisfactory amino acid of homogeneity
With the dry blood cake quality-control product of fatty acyl carnitine and preparation method thereof.
The technical solution used in the present invention is：
A kind of amino acid and the dry blood cake quality-control product of fatty acyl carnitine, mainly by amino acid standard items, fatty acyl carnitine standard items and
Red blood cell it is solid on carrier formed, wherein, amino acid standard items include alanine, phenylalanine, leucine, methionine,
Valine, citrulling, arginine, tyrosine totally 8 kinds of amino acid.
As the further improvement of above-mentioned amino acid and the dry blood cake quality-control product of fatty acyl carnitine, fatty acyl carnitine standard items include trip
From carnitine, acetylcarnitine, propionyl carnitine, butyryl carnitine, C5, C6, C8, certain herbaceous plants with big flowers acylcarnitine, bay
Acylcarnitine, C14, palmitoyl carnitine, 18 phosphinylidyne carnitines, 3- hydroxyls C5, C5DC, the third two
Acylcarnitine totally 15 kinds of fatty acyl carnitines.
As the further improvement of above-mentioned amino acid and the dry blood cake quality-control product of fatty acyl carnitine, in quality-control product each amino acid according to
Coordinated with following Final molar ratios：
As the further improvement of above-mentioned amino acid and the dry blood cake quality-control product of fatty acyl carnitine, each fatty acyl carnitine is pressed in quality-control product
Following Final molar ratios coordinate：
As the further improvement of above-mentioned amino acid and the dry blood cake quality-control product of fatty acyl carnitine, carrier is filter paper.Filter paper can be with
It is the filter paper commonly used in the art such as international 903 filter paper.
The preparation method of a kind of above-mentioned amino acid and the dry blood cake quality-control product of fatty acyl carnitine, comprises the following steps：
1) red blood cell liquid is prepared：Healthy People source whole blood is collected, isolated red blood cell adjusts red blood cell ratio with physiological saline
Hold to predetermined value, obtain red blood cell liquid, and determine wherein each amino acid and fatty acyl carnitine background values；
2) standard liquid is prepared：Amino acid and/or water-soluble fatty acyl carnitine are dissolved using aqueous solvent；It is molten using alcohols
Fat-soluble fatty acyl carnitine is dissolved in agent, obtains amino acid standard liquid and/or fatty acyl carnitine standard liquid；
3) blood cake quality-control product is prepared：Amino acid standard liquid and/or fatty acyl carnitine standard are added in above-mentioned red blood cell liquid
Solution obtains working solution to setting value；Take above-mentioned working solution to be added dropwise on carrier, be dried to obtain amino acid and the dry blood of fatty acyl carnitine
Spot quality-control product.
As the further improvement of above-mentioned preparation method, aqueous solvent is physiological saline, and the amino acid is：Alanine,
Phenylalanine, methionine, valine, citrulling, arginine, leucine and tyrosine；The water-soluble fatty acyl carnitine is：Trip
From carnitine, acetylcarnitine, propionyl carnitine, butyryl carnitine, C5, C6, C8, certain herbaceous plants with big flowers acylcarnitine, 3- hydroxyls
Base C5, C5DC and C3DC；Alcohols solvent is methanol, and the fat-soluble fatty acyl carnitine is：Bay
Acylcarnitine, C14, palmitoyl carnitine, 18 phosphinylidyne carnitines.
As the further improvement of above-mentioned preparation method, prepare in red blood cell liquid step, the predetermined value of hematocrit value is
45~55%.
As the further improvement of above-mentioned preparation method, in working solution, the concentration of each amino acid is respectively：Alanine 350
~650 μM, 120~300 μM of phenylalanine, 150~450 μM of leucine, 50~170 μM of methionine, the μ of valine 200~400
M, 40~150 μM of citrulling, 60~200 μM of arginine, 180~370 μM of tyrosine；The concentration of each fatty acyl carnitine is respectively：Trip
30~70 μM from carnitine, 20~40 μM of acetylcarnitine, 5~12 μM of propionyl carnitine, 1~3.5 μM of butyryl carnitine, C5
0.5~2.0 μM, 0.4~1.2 μM of C6,0.4~1.2 μM of C8,0.4~1.2 μM of certain herbaceous plants with big flowers acylcarnitine, lauroyl meat
0.3~1.0 μM of alkali, 0.4~1.4 μM of C14,4.0~12.0 μM of palmitoyl carnitine, 18 phosphinylidyne carnitines 1.5~
6.0 μM, 0.4~1.2 μM of 3- hydroxyls C5,1.0~3.0 μM of C5DC, 0.2~1.0 μM of C3DC.
As the further improvement of above-mentioned preparation method, prepare in blood cake quality-control product step, take 70~100 μ L work drops
It is added on carrier.
The beneficial effects of the invention are as follows：
The amino acid of the present invention and the dry blood cake quality-control product of fatty acyl carnitine, by well-designed component, consider detection side
The characteristics of method and 18 kinds of amino acid are most representative with being selected after the importance of 45 kinds of fatty acyl carnitine Testing index, significantly
In the case of reducing standard items use, all satisfactory amino acid of stability, homogeneity and the dry blood cake of fatty acyl carnitine are obtained
Quality-control product.
Equally it is the characteristics of considering detection method and each also, the concentration of the quality control index is also very crucial
Selected after the characteristics of Testing index, with optimal representativeness and stability.
The quality-control product preparation method cost is low, simple to operate, larger amount of can be prepared, usage cycles are longer, no
Quality Control lot number need to be frequently changed, facilitates daily Analysis of quality control, so as to meet demand of the clinical and routine testing to quality-control product.
Amino acid made from the preparation method provided using the present invention and bar of the dry blood cake quality-control product of fatty acyl carnitine at -20 DEG C
It can be preserved more than 4 months under part, can meet quality-control product from the homogeneity after -20 DEG C of conditions taking-up recovery normal temperature, stability and want
Ask.
Embodiment
A kind of amino acid and the dry blood cake quality-control product of fatty acyl carnitine, mainly by amino acid standard items, fatty acyl carnitine standard items and
Red blood cell it is solid on carrier formed, wherein, amino acid standard items include alanine, phenylalanine, leucine, methionine,
Valine, citrulling, arginine, tyrosine totally 8 kinds of amino acid.
As the further improvement of above-mentioned amino acid and the dry blood cake quality-control product of fatty acyl carnitine, fatty acyl carnitine standard items include trip
From carnitine, acetylcarnitine, propionyl carnitine, butyryl carnitine, C5, C6, C8, certain herbaceous plants with big flowers acylcarnitine, bay
Acylcarnitine, C14, palmitoyl carnitine, 18 phosphinylidyne carnitines, 3- hydroxyls C5, C5DC, the third two
Acylcarnitine totally 15 kinds of fatty acyl carnitines.
As the further improvement of above-mentioned amino acid and the dry blood cake quality-control product of fatty acyl carnitine, in quality-control product each amino acid according to
Coordinated with following Final molar ratios：
As the further improvement of above-mentioned amino acid and the dry blood cake quality-control product of fatty acyl carnitine, each fatty acyl carnitine is pressed in quality-control product
Following Final molar ratios coordinate：
As the further improvement of above-mentioned amino acid and the dry blood cake quality-control product of fatty acyl carnitine, carrier is filter paper.Filter paper can be with
It is the filter paper commonly used in the art such as international 903 filter paper.
The preparation method of a kind of above-mentioned amino acid and the dry blood cake quality-control product of fatty acyl carnitine, comprises the following steps：
1) red blood cell liquid is prepared：Healthy People source whole blood is collected, isolated red blood cell adjusts red blood cell ratio with physiological saline
Hold to predetermined value, obtain red blood cell liquid, and determine wherein each amino acid and fatty acyl carnitine background values；
2) standard liquid is prepared：Amino acid and/or water-soluble fatty acyl carnitine are dissolved using aqueous solvent；It is molten using alcohols
Fat-soluble fatty acyl carnitine is dissolved in agent, obtains amino acid standard liquid and/or fatty acyl carnitine standard liquid；
3) blood cake quality-control product is prepared：Amino acid standard liquid and/or fatty acyl carnitine standard are added in above-mentioned red blood cell liquid
Solution obtains working solution to setting value；Take above-mentioned working solution to be added dropwise on carrier, be dried to obtain amino acid and the dry blood of fatty acyl carnitine
Spot quality-control product.
As the further improvement of above-mentioned preparation method, aqueous solvent is physiological saline, and the amino acid is：Alanine,
Phenylalanine, methionine, valine, citrulling, arginine, leucine and tyrosine；The water-soluble fatty acyl carnitine is：Trip
From carnitine, acetylcarnitine, propionyl carnitine, butyryl carnitine, C5, C6, C8, certain herbaceous plants with big flowers acylcarnitine, 3- hydroxyls
Base C5, C5DC and C3DC；Alcohols solvent is methanol, and the fat-soluble fatty acyl carnitine is：Bay
Acylcarnitine, C14, palmitoyl carnitine, 18 phosphinylidyne carnitines.
Alcohols solvent also includes the aqueous solution of alcohol.
As the further improvement of above-mentioned preparation method, prepare in red blood cell liquid step, the predetermined value of hematocrit value is
45~55%.
As the further improvement of above-mentioned preparation method, in working solution, the concentration of each amino acid is respectively：Alanine 350
~650 μM, 120~300 μM of phenylalanine, 150~450 μM of leucine, 50~170 μM of methionine, the μ of valine 200~400
M, 40~150 μM of citrulling, 60~200 μM of arginine, 180~370 μM of tyrosine；The concentration of each fatty acyl carnitine is respectively：Trip
30~70 μM from carnitine, 20~40 μM of acetylcarnitine, 5~12 μM of propionyl carnitine, 1~3.5 μM of butyryl carnitine, C5
0.5~2.0 μM, 0.4~1.2 μM of C6,0.4~1.2 μM of C8,0.4~1.2 μM of certain herbaceous plants with big flowers acylcarnitine, lauroyl meat
0.3~1.0 μM of alkali, 0.4~1.4 μM of C14,4.0~12.0 μM of palmitoyl carnitine, 18 phosphinylidyne carnitines 1.5~
6.0 μM, 0.4~1.2 μM of 3- hydroxyls C5,1.0~3.0 μM of C5DC, 0.2~1.0 μM of C3DC.
As the further improvement of above-mentioned preparation method, prepare in blood cake quality-control product step, take 70~100 μ L work drops
It is added on carrier.
Amino acid, fatty acyl carnitine, which both can individually dissolve, is configured as corresponding standard solution, can also be as needed
Multiple standards product are dissolved in the lump, or are divided into multigroup dissolving.It is preferred that, according to different aminoacids, the dissolution characteristics of fatty acyl carnitine
Difference carries out packet dissolving, can so greatly reduce the amount of preparation of standard solution, reduces the difficulty prepared.
During preparation, the target ammonia in U.S. CDC quality-control product concentration, laboratory clinical reference value and red blood cell liquid may be referred to
Base acid and fatty acyl carnitine background concentration, add target amino acid and fatty acyl carnitine standard items, and concentration is adjusted to aimed concn, tool
Body is as follows：
Standard items consumption needed for compound concentration quality-control product needed for calculating
N (amount of theoretical material)=C (needing the concentration strengthened) × V (the Quality Control cumulative volume of preparation)
M (the actually amount of weighing)=n (amount of theoretical material) * M (relative molecular mass)
The cumulative volume of standard substance solution is added needed for calculating, physiological saline volume is added needed for calculating, produces 50% red
Specific volume of cell Quality Control matrix；
V (physiological saline volume)=V1 (erythrocyte volume)-V2 (adds standard substance overall solution volume).
Those skilled in the art can also use other to be prepared for means well known to those skilled in the art.
Concentration unit μM refers to μm ol/L.
With reference to embodiment, technical scheme is further illustrated.
Embodiment 1
1) separation of red blood cell：
Collect Healthy People source anticoagulant heparin whole blood, it is desirable to without obvious haemolysis, jaundice, fat or pollution, with 3000r/min's
Supernatant and tunica albuginea are separated after centrifugation 5min, centrifugation, the physiological saline of about red cell volume equivalent is added, is filled using vortex mixer
Divide and mix 5-7min, separates supernatant and tunica albuginea after 3000r/min centrifugations 5min, repeat this step 3 time, produce red blood cell.Make
It is 50% with physiological saline adjustment hematocrit value, obtains red blood cell liquid, determine amino acid and fatty acyl carnitine sheet in red blood cell liquid
Bottom concentration.
2) preparation of amino acid and fatty acyl carnitine：
Use physiological saline solution alanine Ala, phenylalanine Phe, leucine Lue, methionine Met, valine
Val, citrulling Cit, arginine Arg, tyrosine Tyr totally 8 kinds of amino acid standard items, obtain Freamine Ⅲ；
Use physiological saline solution free carnitine C0, acetylcarnitine C2, propionyl carnitine C3, butyryl carnitine C4, isovaleryl meat
Alkali C5, C6 C6, C8 C8, certain herbaceous plants with big flowers acylcarnitine C10,3- hydroxyl C5 C5-OH, C5DC C5DC,
C3DC C3DC, obtains water-soluble fatty acyl carnitine solution；
Use 40v/v% methanol-waters dissolving Laurylcarnitine C12, C14 C14, palmitoyl carnitine C16,18
Phosphinylidyne carnitine C18, obtains fat-soluble fatty acyl carnitine solution.
Freamine Ⅲ and water-soluble fatty acyl carnitine solution are mixed with red blood cell liquid, fat-soluble fatty acyl carnitine is added dropwise afterwards
Solution, fully mixes, obtains mixed liquor.According to amino acid in red blood cell liquid and fatty acyl carnitine background concentration, by adjusting amino
The addition of the addition and physiological saline of acid solution and fatty acyl carnitine solution, control amino acid and fatty acyl carnitine solution are mixed
The final concentration closed in liquid is as shown in table 1：
Table 1, target amino acid and carnitine configuration concentration scope
3) preparation of dry blood cake quality-control product
The μ L of mixed liquor 85 in previous step are drawn using pipettor, are added dropwise on international 903# filter papers, at room temperature mistake
Night dries, and obtains amino acid and the dry blood cake quality-control product of fatty acyl carnitine.
Amino acid and the dry blood cake quality-control product of fatty acyl carnitine are sealed using valve bag, are placed in less than -20 DEG C, humidity 30RH% with
Preserved under conditions of lower.
With reference to experiment, further amino acid made from displaying the inventive method and the dry blood cake quality-control product of fatty acyl carnitine
Advantage.
Amino acid and the dry blood cake homogeneity of fatty acyl carnitine Quality Control are assessed
Amino acid and each 20 blood cakes of the dry blood cake Quality Control of fatty acyl carnitine are randomly selected, according to routine testing flow processing sample
Product, are measured using LC-MS/MS, calculate RSD.Experimental result is as shown in 2~table of table 5：
Dry blood cake Quality Control (Low) homogeneity of the amino acid of table 2 assesses data
Dry blood cake Quality Control (High) homogeneity of the amino acid of table 3 assesses data
Dry blood cake Quality Control (Low) homogeneity of the fatty acyl carnitine of table 4 assesses data
Dry blood cake Quality Control (High) homogeneity of the fatty acyl carnitine of table 5 assesses data
Experimental data shows：The RSD of amino acid is between 4.00%~15.00% in quality-control product, the RSD of fatty acyl carnitine
Between 5.00%~20.00%, illustrate that Quality Control matrix is uniform, homogeneity is good.
Amino acid and the dry blood cake stability assessment of fatty acyl carnitine Quality Control
Blood cake is dried after preservation, and experiment was using the result of 3 days every time, and 4 groups of Quality Controls of detection daily, every 60 days, repeat this
Operation, calculates its average, RSD, and carries out analysis of trend, using initial 3 days averages as initial value, calculates the rate of recovery.As a result such as
Shown in table 6~9：
Dry blood cake Quality Control (Low) the stability assessment data of table 6, amino acid
Dry blood cake Quality Control (High) the stability assessment data of table 7, amino acid
Dry blood cake Quality Control (Low) the stability assessment data of table 8, fatty acyl carnitine
Dry blood cake Quality Control (High) the stability assessment data of table 9, fatty acyl carnitine
Amino acid and the dry blood cake Quality Control long-time stability of fatty acyl carnitine are verified using LC-MS/MS, wherein amino acid
The rate of recovery is between 80%~110%, and the fatty acyl carnitine rate of recovery is between 80%~120, and amino acid and fatty acyl carnitine are dry
Blood cake quality-control product is below -20 DEG C, under conditions of below humidity 30RH%, can stablize and preserve 4 months.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to assert
The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (10)
1. a kind of amino acid and the dry blood cake quality-control product of fatty acyl carnitine, mainly by amino acid standard items, fatty acyl carnitine standard items and red
Cell is solid in formation on carrier, wherein, the amino acid standard items include alanine, phenylalanine, leucine, first sulphur ammonia
Acid, valine, citrulling, arginine, tyrosine totally 8 kinds of amino acid.
2. amino acid according to claim 1 and the dry blood cake quality-control product of fatty acyl carnitine, it is characterised in that：The fatty acyl carnitine
Standard items include free carnitine, acetylcarnitine, propionyl carnitine, butyryl carnitine, C5, C6, C8, certain herbaceous plants with big flowers
Acylcarnitine, Laurylcarnitine, C14, palmitoyl carnitine, 18 phosphinylidyne carnitines, 3- hydroxyls C5, penta 2
Acylcarnitine, C3DC totally 15 kinds of fatty acyl carnitines.
3. amino acid according to claim 1 or 2 and the dry blood cake quality-control product of fatty acyl carnitine, it is characterised in that：The Quality Control
Each amino acid coordinates according to following Final molar ratios in product：
4. amino acid according to claim 3 and the dry blood cake quality-control product of fatty acyl carnitine, it is characterised in that：Each acyl in quality-control product
Base carnitine is coordinated by following Final molar ratios：
5. amino acid according to claim 1 or 2 and the dry blood cake quality-control product of fatty acyl carnitine, it is characterised in that：The carrier
For filter paper.
6. the preparation method of the amino acid and the dry blood cake quality-control product of fatty acyl carnitine described in a kind of any one of claim 1-5, including
Following steps：
1) red blood cell liquid is prepared：Healthy People source whole blood is collected, isolated red blood cell adjusts hematocrit value extremely with physiological saline
Predetermined value, obtains red blood cell liquid, and determine wherein each amino acid and fatty acyl carnitine background values；
2) standard liquid is prepared：Amino acid and/or water-soluble fatty acyl carnitine are dissolved using aqueous solvent；It is molten using alcohols solvent
Fat-soluble fatty acyl carnitine is solved, amino acid standard liquid and/or fatty acyl carnitine standard liquid is obtained；
3) blood cake quality-control product is prepared：Amino acid standard liquid and/or fatty acyl carnitine standard liquid are added in above-mentioned red blood cell liquid
To setting value, working solution is obtained；Take above-mentioned working solution to be added dropwise on carrier, be dried to obtain amino acid and the dry blood cake matter of fatty acyl carnitine
Control product.
7. preparation method according to claim 6, it is characterised in that：The aqueous solvent is physiological saline, the amino
Acid is：Alanine, phenylalanine, methionine, valine, citrulling, arginine, leucine and tyrosine；The water solubility
Fatty acyl carnitine is：Free carnitine, acetylcarnitine, propionyl carnitine, butyryl carnitine, C5, C6, C8,
Certain herbaceous plants with big flowers acylcarnitine, 3- hydroxyls C5, C5DC and C3DC；
The alcohols solvent is methanol, and the fat-soluble fatty acyl carnitine is：Laurylcarnitine, C14, palmityl meat
Alkali, 18 phosphinylidyne carnitines.
8. preparation method according to claim 6, it is characterised in that：It is described to prepare in red blood cell liquid step, red blood cell ratio
The predetermined value of appearance is 45~55%.
9. the preparation method according to claim 6 or 7, it is characterised in that：In the working solution, the concentration point of each amino acid
It is not：350~650 μM of alanine, 120~300 μM of phenylalanine, 150~450 μM of leucine, 50~170 μM of methionine,
200~400 μM of valine, 40~150 μM of citrulling, 60~200 μM of arginine, 180~370 μM of tyrosine；
In the working solution, the concentration of each fatty acyl carnitine is respectively：30~70 μM of free carnitine, 20~40 μM of acetylcarnitine, third
5~12 μM of acylcarnitine, 1~3.5 μM of butyryl carnitine, 0.5~2.0 μM of C5,0.4~1.2 μM of C6, decoyl meat
0.4~1.2 μM of alkali, 0.4~1.2 μM of certain herbaceous plants with big flowers acylcarnitine, 0.3~1.0 μM of Laurylcarnitine, 0.4~1.4 μM of C14,
4.0~12.0 μM of palmitoyl carnitine, 1.5~6.0 μM of 18 phosphinylidyne carnitine, 0.4~1.2 μM of 3- hydroxyls C5, penta 2
1.0~3.0 μM of acylcarnitine, 0.2~1.0 μM of C3DC.
10. preparation method according to claim 9, it is characterised in that：It is described to prepare in blood cake quality-control product step, take 70~
100 μ L working solutions are added dropwise on carrier.
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| CN107955833A (en) * | 2017-11-20 | 2018-04-24 | 广州市达瑞生物技术股份有限公司 | A kind of dry blood cake positive quality control product of heterozygous mutant for being suitable for deaf mutator detection |
| CN108008031A (en) * | 2017-11-17 | 2018-05-08 | 深圳华大生命科学研究院 | Detect amino acid, carnitine, ketone, the method for hormone and kit at the same time |
| CN109870535A (en) * | 2017-12-01 | 2019-06-11 | 中国科学院大连化学物理研究所 | A kind of extensive qualitative method of fatty acyl carnitine based on mixed biologic sample |
| US10656059B2 (en) | 2018-03-07 | 2020-05-19 | Alcala Pharmaceutical, Inc. | Method for qualitative and quantitative multiplexing of drug analytes from biological samples |
| CN114076701A (en) * | 2021-07-22 | 2022-02-22 | 上海安谱实验科技股份有限公司 | Dried blood spot quality control product for newborn screening and preparation method thereof |
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| 高健群等: "窒息新生儿血肉碱谱与脑损伤相关性的研究", 《中国妇幼保健》 * |
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| CN109870535A (en) * | 2017-12-01 | 2019-06-11 | 中国科学院大连化学物理研究所 | A kind of extensive qualitative method of fatty acyl carnitine based on mixed biologic sample |
| US10656059B2 (en) | 2018-03-07 | 2020-05-19 | Alcala Pharmaceutical, Inc. | Method for qualitative and quantitative multiplexing of drug analytes from biological samples |
| US11054349B2 (en) | 2018-03-07 | 2021-07-06 | Alcala Pharmaceutical, Inc. | Method for preparation of dried blood sample for multiplexing of analytes |
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