CN107142215A - A kind of ganoderma lucidum tissue disintegration culture medium and preparation method thereof - Google Patents
A kind of ganoderma lucidum tissue disintegration culture medium and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of ganoderma lucidum tissue disintegration culture medium and preparation method thereof, the culture medium is mainly made up of glucose, yeast extract, potato, peptone, agar, hawthorn, malt, matrimony vine, smilax, dregs of beans, Herba Lespedezae Cuneatae, corncob, vinasse, gypsum, tea grounds, the capsule of weeping forsythia, salt and water.Active ingredient polyoses content in the ganoderma lucidum quality better that the medium culture comes out, ganoderma lucidum is high.
Description
Technical field
The present invention relates to a kind of Medium for Ganoderma lucidum and preparation method thereof, particularly a kind of ganoderma lucidum tissue disintegration culture medium and
Its preparation method.
Background technology
Ganoderma lucidum is the drying fructification of On Polyporaceae red sesame or purple sesame.Ganoderma lucidum is a kind of precious medicinal fungi, category
In Basidiomycetes Polyporaceae Ganoderma, there is the medicinal history of more than 2,000 years in China.Ganoderma lucidum first recorded in《Sheng Nong's herbal classic》, the successive dynasties
Doctor thinks that ganoderma lucidum can treat a variety of diseases, is the precious medicine that strengthening by means of tonics is strengthened the body resistance to consolidate the constitution.GL-B be in ganoderma lucidum most
One of effective composition, is present in the fructification of ganoderma lucidum, conidia powder and mycelium, with suppression tumour, enhancing body to certainly
The ability removed by base, thus can reduce free radical to the damage of body, have the effect of anti-aging, can also improve immunity,
Anti-inflammatory, reducing blood lipid, hypoglycemic etc. are acted on.
As the health-care efficacy of ganoderma lucidum is widely recognized and received by people, Wild ganoderma far can not meet people's
Demand.Culture medium is ganoderma lucidum conditions on which persons or things depend for existence, different culture raw materials and with can to the mycelial growth of ganoderma lucidum, yield and
Active component etc. produces important influence.Wherein, ganoderma lucidum tissue disintegration culture medium influences on the quality of the finished product ganoderma lucidum in later stage
It is very big.In the prior art in the culture medium during ganoderma lucidum tissue disintegration during raw material such as cotton seed hulls, wood chip, these raw materials often account for total amount
50%-80%, be primary raw material, the raw material proportion such as wheat bran is less, and generally in 10%-20%, but these raw materials are sought in itself
Form point few, be unfavorable for the quality guarantee of output ganoderma lucidum.Particularly it cannot be guaranteed that in ganoderma lucidum polysaccharide content so that finally give
Ganoderma lucidum in polyoses content it is low.
The content of the invention
The purpose of the present invention, is to provide a kind of ganoderma lucidum tissue disintegration culture medium and preparation method thereof.The culture medium training
The active ingredient polyoses content raised in the ganoderma lucidum quality better come, ganoderma lucidum is high.
What the present invention was realized in.
A kind of ganoderma lucidum tissue disintegration culture medium, the culture medium is calculated by weight, mainly by 10-30 parts of glucose,
1-5 parts of yeast extract, 150-250 parts of potato, 1-5 parts of peptone, 15-35 parts of agar, 25-35 parts of hawthorn, 1-10 parts of malt, matrimony vine
5-15 parts, 20-30 parts of smilax, 45-55 parts of dregs of beans, 20-40 parts of Herba Lespedezae Cuneatae, 20-40 parts of corncob, 10-20 parts of vinasse, gypsum
900-1100 parts of 10-30 parts, 25-45 parts of tea grounds, 35-45 parts of the capsule of weeping forsythia, 5-15 parts of salt and water are made.
Foregoing ganoderma lucidum tissue disintegration culture medium, the culture medium is calculated by weight, mainly by glucose 15-25
Part, 1.5-2.5 parts of yeast extract, 180-220 parts of potato, 1.5-2.5 parts of peptone, 20-24 parts of agar, 28-32 parts of hawthorn, malt
2-8 parts, 8-12 parts of matrimony vine, 22-28 parts of smilax, 48-52 parts of dregs of beans, 25-35 parts of Herba Lespedezae Cuneatae, 25-35 parts of corncob, vinasse
950-1050 parts of 12-18 parts, 15-25 parts of gypsum, 30-40 parts of tea grounds, 38-42 parts of the capsule of weeping forsythia, 8-12 parts of salt and water are made.
Foregoing ganoderma lucidum tissue disintegration culture medium, the culture medium is calculated by weight, mainly by 20 parts of glucose, ferment
Female 2 parts of cream, 200 parts of potato, 1-2 parts of peptone, 22 parts of agar, 30 parts of hawthorn, 5 parts of malt, 10 parts of matrimony vine, 25 parts of smilax,
50 parts of dregs of beans, 30 parts of Herba Lespedezae Cuneatae, 30 parts of corncob, 15 parts of vinasse, 20 parts of gypsum, 35 parts of tea grounds, 40 parts of the capsule of weeping forsythia, 10 parts of salt and
1000 parts of water is made.
A kind of preparation method of foregoing ganoderma lucidum tissue disintegration culture medium, potato, hawthorn, matrimony vine, smilax, snake fall
Move back, the capsule of weeping forsythia, tea grounds and corncob, chopping, plus formula ratio 40-60% water boiled 20-30 minute, and filter residue is discarded, and filtrate, which adds, to be remained
Excess water, continues to be heated to after 50-60 degree, adds remaining raw material, stirring while adding, after whole raw materials are added, and continues to heat
30-50min, packing, silica gel plug sealing is wrapped, and after being sterilized 20-30 minutes under 120-122 degree, is cooled to 50-60 degree, is tilted 5-
15 degree are placed, and at 30-35 DEG C after drying 24-48h, are taken out, are preserved, produce.
The preparation method of foregoing ganoderma lucidum tissue disintegration culture medium, potato, hawthorn, matrimony vine, smilax, Herba Lespedezae Cuneatae, company
Stick up, tea grounds and corncob, chopping, plus the water of formula ratio 50% boiled 25 minutes, and filter residue is discarded, and filtrate adds surplus water, is continued
It is heated to after 50-60 degree, adds remaining raw material, it is stirring while adding, after whole raw materials are added, continue to heat 40min, dispense,
Silica gel plug is sealed, and is wrapped with newspaper, after being sterilized 25 minutes under 120-122 degree, is cooled to 50-60 degree, tilts 10 degree of placements, 30-
Dry after 35h, take out at 35 DEG C, preserve, produce.
Applicant ganoderma lucidum tissue disintegration has carried out substantial amounts of research with culture medium, and part Experiment is as follows：
Experimental example determination of polysaccharide
1 determines project：
1.1 ganoderma lucidums 1：Selection high-quality Wild ganoderma carries out tissue separation with culture medium of the present invention and then purified, matter is matched somebody with somebody, rejuvenation
（Switching）, expand it is numerous, domestication, cultivation etc. rear harvesting ganoderma lucidum.
1.2 ganoderma lucidums 2：Selection high-quality Wild ganoderma carries out tissue separation with culture medium and then purified, matter is matched somebody with somebody, rejuvenation（Turn
Connect）, expand it is numerous, domestication, cultivation etc. rear harvesting ganoderma lucidum；The culture medium is purchased from longitude and latitude agricultural product Co., Ltd of Suzhou City.
2 methods
The preparation of 2.1 phenol solutions
Accurate weighing analyzes pure phenol crystal 5g in 250m L, adds distilled water 60m L, being put into 50 DEG C of water-baths dissolves it,
The constant volume in 100mL volumetric flasks, produces 5% phenol solution.Phenol can be with oxidation by air, so phenol solution is now with the current.
2.2 sample extraction method of polysaccharides
2.2.1 the sample solution of ganoderma lucidum 1：The sample of 5g red ganodermas 1 is taken, 80 mesh sieves were crushed.Take gained REISH 0.5g in
In 500mL beakers, 15mL95% ethanol is added, is heated 30 minutes in 60 DEG C of constant temperature water baths, filtering.Operated more than repeating
Twice, to remove the interference composition such as monosaccharide and disaccharide and oligosaccharide.Sample after filtering extracts one in 100 DEG C of boiling water
The section time, filtrate plus distilled water is taken to be settled to 500mL after filtering.
2.2.1 the sample solution of ganoderma lucidum 2：The sample of 5g red ganodermas 2 is taken, 80 mesh sieves were crushed.Take gained REISH 0.5g
In 500mL beakers, 15mL95% ethanol is added, is heated 30 minutes in 60 DEG C of constant temperature water baths, filtering.Grasped more than repeating
Make twice, to remove the interference composition such as monosaccharide and disaccharide and oligosaccharide.Sample after filtering is extracted in 100 DEG C of boiling water
For a period of time, filtrate plus distilled water is taken to be settled to 500mL after filtering.
2.3 spectrophotometry polyoses contents
2.3.1 the drafting of standard curve.The abundant dried pure glucose 50mg of analysis of accurate weighing, adds water and is settled to 500mL
In volumetric flask, 0.1mg/mL glucose standards solutions are obtained.6, volumetric flask for taking 50m L clean.It is accurate with pipette respectively
Glucose standards solution 5mL, 10mL, 15mL, 20mL, 25mL, 30mL are added, distilled water constant volume is added, that is, obtains concentration difference
It is molten for 0.01mg/mL, 0.02mg/mL, 0.03mg/mL, 0.04mg/mL, 0.05mg/mL, 0.06mg/mL Glucose standards
Liquid.Take each concentration of glucose standard liquids of 1mL to be added in 20mL color-comparison tubes during measure, enter rapidly 5% phenol solution with
And 98% the concentrated sulfuric acid it is appropriate, place the 20min that develops the color at room temperature after shaking up.Separately take lmL distilled water, ibid add phenol solution and
The concentrated sulfuric acid, does blank reference solution.Absorbance is determined at specified wavelength, standard curve is drawn, calibration curve equation is calculated
And coefficient R.
2.3.2 the determination of polysaccharide of ganoderma lucidum 1：The sample polysaccharide extraction liquid of lm L ganoderma lucidums 1 is taken to be added to 20mL
In color-comparison tube, phenol solution and the concentrated sulfuric acid are sequentially added using with titer colour developing same procedure, in specific wavelength
Place determines absorbance, brings absorbance into calibration curve equation, calculates sample liquid concentration C.Be calculated as follows out take it is many
Sugared content, polyoses content (%)=CV/m, C is sample liquid concentration, and V is sample liquid volume, and m is the quality of sampling.
2.3.3 the determination of polysaccharide of ganoderma lucidum 2：The sample polysaccharide extraction liquid of lm L ganoderma lucidums 2 is taken to be added to 20mL
In color-comparison tube, phenol solution and the concentrated sulfuric acid are sequentially added using with titer colour developing same procedure, in specific wavelength
Place determines absorbance, brings absorbance into calibration curve equation, calculates sample liquid concentration C.Be calculated as follows out take it is many
Sugared content, polyoses content (%)=CV/m, C is sample liquid concentration, and V is sample liquid volume, and m is the quality of sampling.
3 results
The determination of 3.1 maximum absorption wavelengths
LmL 0.05m g/mL glucose standards solutions are taken, are added after phenol solution and the concentrated sulfuric acid after reaction a period of time.
450-520nm range of wavelengths carries out spectral scan, determines its maximum absorption wavelength, finds at 488nm wavelength, gained extinction
Angle value is maximum, without other miscellaneous peaks, then optimum determining wavelength selects 488nm.
The determination of 3.2 phenol solution consumptions
The glucose standard 1mL of same concentrations is taken in 6 20mL color-comparison tubes, be separately added into 0.4mL, 0.6mL,
0.8mL, 1.0mL, 1.2mL, 1.4mL 5% phenol solution, sequentially add the concentrated sulfuric acids of 5mL 98%, and mixing shakes up.Place
Developed the color 30 minutes at a temperature of 20 DEG C, absorbance is determined at 488nm wavelength.When adding phenol solution 1.0mL, absorbance
Value is maximum, and add phenol solution excessively can cause absorbance to reduce on the contrary, thus in experiment 5% phenol solution optimal addn
It should be 1mL.
The determination of 3.3 concentrated sulfuric acid consumptions
The glucose standards solution lmL of same concentrations is taken to be added in the color-comparison tube of five numberings, 5% phenol of each addition is molten
Liquid lmL, is added dropwise the concentrated sulfuric acid 4.0mL, 4.5mL, 5.0mL, 5.5m, 6.0mL successively.Its absorbance is determined at 488nm wavelength, is sent out
Existing sulphuric acid absorbance measured when being 5m L reaches maximum, adds sulfuric acid and crosses and reacts not complete enough at least.Add
Sulfuric acid may excessively cause other side reactions, cause measurement result to reduce, so optimal sulphuric acid is 5mL.
The determination of 3.4 colour temps
Take finite concentration glucose standard lmL to be added in the color-comparison tube of five numberings respectively, 5% is respectively added thereto
Phenol solution lmL, it is rapid that 98% concentrated sulfuric acid 5mL is added dropwise, developed the color at a temperature of 20 DEG C, 30 DEG C, 40 DEG C, 50 DEG C, 60 DEG C are put in respectively
30min, determines its absorbance at 489nm wavelength.At each temperature, the absorbance measured is basically unchanged, so temperature is to anti-
Absorbance is answered without influence, can just to react completely at room temperature substantially.
The determination of 3.5 developing times
Take finite concentration glucose standard lmL to be added in the color-comparison tube of five numberings respectively, 5% is respectively added thereto
Phenol solution lmL, it is rapid that 98% concentrated sulfuric acid 5mL is added dropwise, respectively at room temperature colour developing 10min, 20min, 30min, 40min,
50min, determines its absorbance at 488nm wavelength.It was found that, 10 minutes absorbances of reaction are smaller, and developing time is from 20 to 50
Minute, the absorbance of sample solution is basically unchanged, so reaction time control can just react complete substantially more than 20 minutes.
So the reaction time is controlled more than 20 minutes with regard to that can reach optimum efficiency.
In summary measurement result, draws Phenol sulfuric acid procedure optimum determining condition parameter：1m sample liquids, 5% phenol solution
Addition is 1m L, and 98% dense sulphur, sour addition is 5mL, and colour temp room temperature, developing time is more than 20 minutes, most
Big absorbing wavelength is 488nm.
The drafting of 3.6 standard curves
The abundant dried pure glucose 50mg of analysis of accurate weighing, adds water and is settled in 500mL volumetric flasks, obtain 0.1mg/mL
Glucose standards solution.Take 6, the volumetric flask that 50mL is clean, accurately added with pipette respectively glucose standards solution 5mL,
10mL、15mL、20mL、25mL、30mL.Distilled water constant volume is added, that is, it is respectively 0.01mg/m L, 0.02mg/ to obtain concentration
ML, 0.03mg/mL, 00.04mg/mL, 0.05mg/mL, 0.06mg/mL glucose standards solution.Each concentration of 1mL is taken during measure
Glucose standards solution is added in 20mL color-comparison tubes, is rapidly added 5% phenol solution and 98% concentrated sulfuric acid is appropriate,
The 20min that develops the color at room temperature is placed after shaking up.LmL distilled water separately is taken, phenol solution and the concentrated sulfuric acid is ibid added, does blank ginseng
Compare solution.Absorbance is determined at specified wavelength, standard curve is drawn, calibration curve equation and coefficient R is calculated.Accurately
Weigh glucose 0.0520g.Calibration curve equation be y=9.0803x-0.0385, coefficient R value is 0.9984, linearly compared with
It is good, range of linearity 10.5-63.0ug/mL.
3.7 replica test
Take sample solution to determine 5 times using Phenol sulfuric acid procedure is parallel, obtain data such as table 1.
The repeated experiment result of table 1
| Sequence number | 1 | 2 | 3 | 4 | 5 | RSD/% |
| Absorbance A | 0.241 | 0.248 | 0.245 | 0.246 | 0.242 | 1.18 |
RSD=1.18%, illustrates that precision is higher, can meet the requirement of quantitative analysis, and mean absorbance values are 0.244.
3.8 recovery of standard addition
The sample solution of ganoderma lucidum 1 and the sample solution 0.5mL of ganoderma lucidum 2 are accurately pipetted respectively in 20mL color-comparison tubes, it is accurate respectively
0.005mg/mL, 0.010mg/mL, 0.015mg/mL standard liquid are pipetted, 0.5mL is added in sample liquid, determines polyoses content
Calculate average recovery rate.Average recovery rate is that 97.64%, RSD is 1.2%.The rate of recovery is high, illustrates that this method result accuracy rate is high.
4 assay results
Ganoderma lucidum 1 and ganoderma lucidum 2 are taken respectively, it is parallel to be divided into 6 parts, the sample solution of ganoderma lucidum 1 and the sample solution of ganoderma lucidum 2 is made, by above-mentioned side
Method is measured, record data, results averaged.Obtain the results are shown in Table 2.
The ganoderma polyoses content measurement result of table 2
As seen from table, culture medium of the present invention is carried out after tissue separation, and the content of polysaccharide is higher in the ganoderma lucidum finally harvested.Ganoderma lucidum product
Matter is good.
Compared with prior art, the active ingredient in the ganoderma lucidum quality better that medium culture of the present invention comes out, ganoderma lucidum
Polyoses content is high.
Embodiment
Embodiment 1.
Raw material：Glucose 20kg, yeast extract 2kg, potato 200kg, peptone 1-2kg, agar 22kg, hawthorn 30kg, wheat
Bud 5kg, matrimony vine 10kg, smilax 25kg, dregs of beans 50kg, Herba Lespedezae Cuneatae 30kg, corncob 30kg, vinasse 15kg, gypsum 20kg, tea
Slag 35kg, capsule of weeping forsythia 40kg, salt 10kg and water 1000kg.
Preparation method：Potato, hawthorn, matrimony vine, smilax, Herba Lespedezae Cuneatae, the capsule of weeping forsythia, tea grounds and corncob, chopping, plus formula ratio
50% water is boiled 25 minutes, and filter residue is discarded, and filtrate adds surplus water, continues to be heated to after 50-60 degree, adds remaining original
Material, it is stirring while adding, after whole raw materials are added, continue to heat 40min, packing, silica gel plug sealing is wrapped, 120-122 with newspaper
After the lower sterilizing of degree 25 minutes, 50-60 degree is cooled to, 10 degree is tilted and places, at 30-35 DEG C after drying 35h, takes out, preserves, i.e.,
.
Embodiment 2.
Raw material：Glucose 30kg, yeast extract 5kg, potato 250kg, peptone 5kg, agar 35kg, hawthorn 35kg, malt 10kg,
Matrimony vine 15kg, smilax 30kg, dregs of beans 55kg, Herba Lespedezae Cuneatae 40kg, corncob 40kg, vinasse 20kg, gypsum 30kg, tea grounds
45kg, capsule of weeping forsythia 45kg, salt 15kg and water 1100kg.
4. preparation method：Potato, hawthorn, matrimony vine, smilax, Herba Lespedezae Cuneatae, the capsule of weeping forsythia, tea grounds and corncob, chopping, plus formula
The water of amount 60% is boiled 30 minutes, and filter residue is discarded, and filtrate adds surplus water, continues to be heated to after 50-60 degree, is added remaining
Raw material, it is stirring while adding, after whole raw materials are added, continue to heat 30-50min, packing, silica gel plug sealing is wrapped, 120-122
After the lower sterilizing of degree 30 minutes, 50-60 degree is cooled to, 15 degree is tilted and places, at 30-35 DEG C after drying 48h, takes out, preserves, i.e.,
.
Embodiment 3.
Raw material：Glucose 10kg, yeast extract 1kg, potato 150kg, peptone 1kg, agar 15kg, hawthorn 25kg, malt 1kg,
Matrimony vine 5kg, smilax 20kg, dregs of beans 45kg, Herba Lespedezae Cuneatae 20kg, corncob 20kg, vinasse 10kg, gypsum 10kg, tea grounds 25kg,
Capsule of weeping forsythia 35kg, salt 5kg and water 900kg.
Preparation method：Potato, hawthorn, matrimony vine, smilax, Herba Lespedezae Cuneatae, the capsule of weeping forsythia, tea grounds and corncob, chopping, plus formula ratio
60% water is boiled 30 minutes, and filter residue is discarded, and filtrate adds surplus water, continues to be heated to after 50-60 degree, adds remaining original
Material, it is stirring while adding, after whole raw materials are added, continue to heat 30-50min, packing, silica gel plug sealing is wrapped, 120-122 degree
After lower sterilizing 30 minutes, 50-60 degree is cooled to, is tilted at 15 degree of placements, 30-35 DEG C after drying 48h, taking-up is preserved, produced.
Claims (5)
1. a kind of ganoderma lucidum tissue disintegration culture medium, it is characterised in that：The culture medium is calculated by weight, mainly by glucose
10-30 parts, 1-5 parts of yeast extract, 150-250 parts of potato, 1-5 parts of peptone, 15-35 parts of agar, 25-35 parts of hawthorn, malt 1-
10 parts, 5-15 parts of matrimony vine, 20-30 parts of smilax, 45-55 parts of dregs of beans, 20-40 parts of Herba Lespedezae Cuneatae, 20-40 parts of corncob, vinasse 10-
900-1100 parts of 20 parts, 10-30 parts of gypsum, 25-45 parts of tea grounds, 35-45 parts of the capsule of weeping forsythia, 5-15 parts of salt and water are made.
2. it is as claimed in claim 1 main by 15-25 parts of glucose, 1.5-2.5 parts of yeast extract, 180-220 parts of potato, albumen
1.5-2.5 parts of peptone, 20-24 parts of agar, 28-32 parts of hawthorn, 2-8 parts of malt, 8-12 parts of matrimony vine, 22-28 parts of smilax, dregs of beans
48-52 parts, 25-35 parts of Herba Lespedezae Cuneatae, 25-35 parts of corncob, 12-18 parts of vinasse, 15-25 parts of gypsum, 30-40 parts of tea grounds, the capsule of weeping forsythia
950-1050 parts of 38-42 parts, 8-12 parts of salt and water are made.
3. ganoderma lucidum tissue disintegration culture medium as claimed in claim 1 or 2, it is characterised in that：The culture medium is by weight
Calculate, mainly by 20 parts of glucose, 2 parts of yeast extract, 200 parts of potato, 1-2 parts of peptone, 22 parts of agar, 30 parts of hawthorn, malt 5
Part, 10 parts of matrimony vine, 25 parts of smilax, 50 parts of dregs of beans, 30 parts of Herba Lespedezae Cuneatae, 30 parts of corncob, 15 parts of vinasse, 20 parts of gypsum, tea grounds
1000 parts of 35 parts, 40 parts of the capsule of weeping forsythia, 10 parts of salt and water are made.
4. a kind of preparation method of ganoderma lucidum tissue disintegration culture medium as any one of claim 1-3, its feature exists
In：Potato, hawthorn, matrimony vine, smilax, Herba Lespedezae Cuneatae, the capsule of weeping forsythia, tea grounds and corncob, chopping, plus formula ratio 40-60% water boil
20-30 minutes, filter residue was discarded, and filtrate adds surplus water, continues to be heated to after 50-60 degree, added remaining raw material, side edged
Stirring, after whole raw materials are added, continues to heat 30-50min, packing, silica gel plug sealing is wrapped, sterilize 20- under 120-122 degree
After 30 minutes, 50-60 degree is cooled to, 5-15 degree is tilted and places, at 30-35 DEG C after drying 24-48h, is taken out, is preserved, produce.
5. the preparation method of ganoderma lucidum tissue disintegration culture medium as claimed in claim 4, it is characterised in that：Potato, hawthorn, Chinese holly
Qi, smilax, Herba Lespedezae Cuneatae, the capsule of weeping forsythia, tea grounds and corncob, chopping, plus the water of formula ratio 50% are boiled 25 minutes, and filter residue is discarded, filter
Liquid adds surplus water, continues to be heated to after 50-60 degree, adds remaining raw material, stirring while adding, after whole raw materials are added,
Continue to heat 40min, packing, silica gel plug sealing is wrapped with newspaper, after sterilizing 25 minutes under 120-122 degree, is cooled to 50-60
Degree, is tilted at 10 degree of placements, 30-35 DEG C after drying 35h, taking-up is preserved, produced.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101376873A (en) * | 2007-08-29 | 2009-03-04 | 上海医药工业研究院 | Chinese Ganoderma fermentation method and Chinese Ganoderma mycelium prepared thereby |
| CN104058802A (en) * | 2014-06-27 | 2014-09-24 | 朱正来 | Ganoderma lucidum culture medium, method for cultivating ganoderma lucidum, ganoderma lucidum tea and preparation method of ganoderma lucidum tea |
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2017
- 2017-06-15 CN CN201710453488.7A patent/CN107142215A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101376873A (en) * | 2007-08-29 | 2009-03-04 | 上海医药工业研究院 | Chinese Ganoderma fermentation method and Chinese Ganoderma mycelium prepared thereby |
| CN104058802A (en) * | 2014-06-27 | 2014-09-24 | 朱正来 | Ganoderma lucidum culture medium, method for cultivating ganoderma lucidum, ganoderma lucidum tea and preparation method of ganoderma lucidum tea |
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| Title |
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| 扶亚祥等: "扛板归化学成分分析及抗菌效果研究", 《动物医学进展》 * |
| 韦朝阳等: "外源添加物对食药用菌液体发酵影响的研究进展", 《食品科学》 * |
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Application publication date: 20170908 |