CN107138193B - Digital PCR instrument - Google Patents
Digital PCR instrument Download PDFInfo
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- CN107138193B CN107138193B CN201710287845.7A CN201710287845A CN107138193B CN 107138193 B CN107138193 B CN 107138193B CN 201710287845 A CN201710287845 A CN 201710287845A CN 107138193 B CN107138193 B CN 107138193B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
- B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
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- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Fluid-Driven Valves (AREA)
- Micromachines (AREA)
Abstract
The invention relates to the technical field of biological detection equipment, in particular to a digital PCR instrument, which comprises: an operation platform for placing a microfluidic chip, the microfluidic chip having a fluid channel, and the fluid channel having a channel inlet and a channel outlet; the microfluidic chip is provided with a first flexible valve body communicated with the channel inlet and a second flexible valve body communicated with the channel outlet; a first actuator; a second actuator; the positioning frame is used for placing the first adaptive pipe and the second adaptive pipe; a first air pressure generating mechanism; a second air pressure generating mechanism; a master control device. The embodiment of the invention realizes the sample injection of the microfluidic chip and has the filling uniformity by utilizing the microfluidic chip with the channel inlet and the channel outlet, the first flexible valve body arranged at the channel inlet, the second flexible valve body arranged at the channel outlet, the first actuating mechanism, the second actuating mechanism, the first air pressure generating mechanism, the second air pressure generating mechanism and the main control equipment.
Description
Technical Field
The invention relates to the technical field of biological detection equipment, in particular to a digital PCR instrument.
Background
Digital polymerase chain reaction (dPCR) is used as a new DNA quantification technology, and single-molecule DNA absolute quantification is realized. At present, the method plays an important role in clinical diagnosis, transgenic component quantification, single cell gene expression, environmental microorganism detection, next generation sequencing and other aspects. In the digital PCR sample injection process, microfluid needs to be injected into a microfluid chip for subsequent detection. The microfluid chip technology is one of the high and new technologies which are rapidly developed at present and the advanced fields of multidisciplinary cross science and technology, and is an important technical platform for researching signal detection and processing methods in life science, chemical science and information science.
In the injection step of the microfluidic chip, fluid driving and control are critical and can be divided into pressure driving, electric driving, thermal driving and surface tension driving, wherein the pressure driving is the mainstream technology of the fluid driving. At present, the pressure driving is mainly to press fluid into a chip by positive pressure, and the process is simple, namely, the sample is squeezed into a microfluidic channel of the chip by increasing the pressure of the fluid sample, but with the reduction of the size of the microfluidic chip and the gradual complexity of the microfluidic channel, the following disadvantages are exposed in the positive pressure mode: first, the flow resistance experienced during fluid injection is large, increasing the difficulty of sample injection. Secondly, after the fluid sample is injected, the gas residue possibility in the fluid channel is high, the sample is difficult to press into the volume occupied by the residual gas, the uniformity of the full filling of the sample is difficult to realize, and even the sample injection failure is caused; thirdly, the positive pressure mode generally requires a certain amount of sample to realize smooth sample introduction, which causes great waste to some precious reagents or samples. In order to solve the problem, in the prior art, the flow is controlled by using a micro valve and a micro pump, but the system has high cost and has strict requirements on fluid pressure, flow rate and the like.
Disclosure of Invention
The invention aims to provide a digital PCR instrument for injecting samples by a microfluid chip, which can realize positive pressure drive and negative pressure drive and can realize the uniformity of filling the microfluid chip with injected samples.
In order to solve the above technical problems, an embodiment of the present invention provides a digital PCR instrument, including:
an operation platform for placing a microfluidic chip, the microfluidic chip having a fluid channel, and the fluid channel having a channel inlet and a channel outlet; the microfluidic chip is provided with a first flexible valve body communicated with the channel inlet and a second flexible valve body communicated with the channel outlet;
the first actuating mechanism is used for extruding the first flexible valve body, and the inlet of the channel is sealed after the first flexible valve body is extruded;
the second actuating mechanism is used for extruding the second flexible valve body, and the second flexible valve body seals the channel outlet after being extruded;
the positioning frame is used for placing a first adaptive pipe and a second adaptive pipe, wherein the first adaptive pipe is used for being communicated with the first flexible valve body, and the second adaptive pipe is used for being communicated with the second flexible valve body;
a first air pressure generating mechanism for generating air pressure to the first adaptive pipe;
a second air pressure generating mechanism for generating air pressure to the second adaptive pipe;
the main control device is used for respectively controlling the first executing mechanism, the second executing mechanism, the first air pressure generating mechanism and the second air pressure generating mechanism.
Compared with the prior art, the embodiment of the invention realizes the sample introduction of the microfluidic chip by utilizing the microfluidic chip with the channel inlet and the channel outlet, the first flexible valve body arranged at the channel inlet, the second flexible valve body arranged at the channel outlet, the first executing mechanism, the second executing mechanism, the first air pressure generating mechanism, the second air pressure generating mechanism and the main control equipment. Specifically, the first flexible valve body is extruded by the first actuating mechanism and is matched with the second actuating mechanism to extrude the second flexible valve body, so that the on-off of a channel inlet and a channel outlet can be selectively controlled, the microfluidic chip can be selectively closed, and then a first adaptive pipe communicated with the first flexible valve body, a second adaptive pipe communicated with the second flexible valve body, a first air pressure generating mechanism used for generating air pressure to the first adaptive pipe and a second air pressure generating mechanism used for generating air pressure to the second adaptive pipe are matched, and a microfluidic sample is injected into the fluid channel by utilizing positive pressure or negative pressure generated by the first air pressure generating mechanism and the second air pressure generating mechanism and has full uniformity.
Preferably, in the digital PCR instrument, the operation platform is provided with a socket, and the socket is matched with the microfluidic chip in shape and is used for inserting the microfluidic chip so as to fix the microfluidic chip.
Preferably, in the digital PCR instrument, the socket groove includes a sliding groove portion extending to an edge of the operation platform, so that the microfluidic chip is more easily mounted.
Preferably, in the digital PCR instrument, the positioning frame includes:
the first fixing part is used for fixing the first flexible valve body;
and the second fixing part is used for fixing the second flexible valve body. The locating rack is fixed first flexible valve body and the flexible valve body of second in fixed first fitting pipe and the second fitting pipe for the connection of first fitting pipe and first flexible valve body, and the connection of second fitting pipe and the flexible valve body of second is more firm, and utilizes same locating rack, makes the structure of this digital PCR appearance more compact.
Preferably, in the digital PCR instrument, the first executing mechanism includes a first pressing thimble and a first telescopic device, the first telescopic device is configured to drive the first pressing thimble to move, and after the first pressing thimble presses the first flexible valve body, the inlet of the channel is closed. The structure is relatively simple, the sealing of the channel inlet can be completely realized, and the first telescopic device is matched with the main control equipment to realize remote operation and is more practical.
Preferably, in the digital PCR instrument, the operating platform is provided with a first hollow portion, and the first pressing thimble penetrates through the first hollow portion, so that the structure of the digital PCR instrument is more compact.
Preferably, in the digital PCR instrument, the first flexible valve body has a first internal passage and a first pressing groove, the first pressing groove extends toward the first internal passage, and the first pressing groove is used for the first pressing spike to extend into. The wall thickness of the pressing part of the first flexible valve body is reduced, so that the first flexible valve body is easier to deform, and the inlet of the channel is convenient to close.
Preferably, in the digital PCR instrument, the first flexible valve body further has a first supporting groove extending toward the first internal passage;
the locating rack further comprises a first supporting ejector pin which is used for extending into the first supporting groove, and the first supporting ejector pin and the first pressing ejector pin are arranged oppositely. The wall thickness of the pressed part of the first flexible valve body is further reduced, and a supporting force is provided for the first pressing thimble, so that the first flexible valve body is easier to deform, and the inlet of the channel is closed.
Preferably, in the digital PCR instrument, the second actuator includes a second pressing thimble and a second telescopic device, the second telescopic device is configured to drive the second pressing thimble to move, and after the second pressing thimble presses the second flexible valve body, the channel outlet is closed. The structure is relatively simple, the sealing of the channel inlet can be completely realized, and the second telescopic device is matched with the main control equipment to realize remote operation, so that the structure is more practical.
Preferably, in the digital PCR instrument, the operating platform is provided with a second hollow portion, and the second pressing thimble penetrates through the second hollow portion, so that the structure of the digital PCR instrument is more compact.
Preferably, in the digital PCR instrument, the second flexible valve body has a second internal channel and a second pressing groove, the second pressing groove extends toward the second internal channel, and the second pressing groove is used for the second pressing spike to extend into. The wall thickness of the pressed part of the second flexible valve body is reduced, so that the second flexible valve body is easier to deform, and the inlet of the channel is closed.
Preferably, in the digital PCR instrument, the second flexible valve body further has a second support groove extending toward the second internal passage;
the positioning frame further comprises a second supporting thimble used for extending into the second supporting groove, and the second supporting thimble and the second pressing thimble are arranged oppositely. The wall thickness of the pressed part of the second flexible valve body is further reduced, and a supporting force is provided for the second pressing thimble, so that the second flexible valve body is easier to deform, and the inlet of the channel is closed.
Preferably, in the digital PCR instrument, the first air pressure generating mechanism includes a first air pressure generating device and a first joint communicated with the first air pressure generating device;
the digital PCR instrument further comprises a first movable plate, the first joint is arranged on the first movable plate, and the first movable plate moves relative to the positioning frame through a first stroke mechanism and is used for driving the first joint to be communicated with the first adapting pipe. The operation flexibility of the digital PCR instrument is improved, and the first adaptive pipe is convenient to replace.
Preferably, in the digital PCR instrument, the second air pressure generating mechanism includes a second air pressure generating device and a second joint communicated with the second air pressure generating device;
the digital PCR instrument further comprises a second movable plate, the second joint is arranged on the second movable plate, and the second movable plate moves relative to the positioning frame through a second stroke mechanism and is used for driving the second joint to be communicated with the second adapter tube. The operation flexibility of the digital PCR instrument is improved, and the second adapter tube is convenient to replace.
Drawings
FIG. 1 is a first schematic structural diagram of a digital PCR instrument according to a first embodiment of the present invention;
FIG. 2 is a second schematic structural view of a digital PCR instrument according to a first embodiment of the present invention;
FIG. 3 is a third schematic structural view of a digital PCR instrument according to a first embodiment of the present invention;
FIG. 4 is a schematic view of the internal structure of a microfluidic chip in a first embodiment of the present invention;
FIG. 5 is a schematic structural view of a spacer according to a first embodiment of the present invention;
FIG. 6 is a first schematic structural view of a microfluidic chip according to a first embodiment;
FIG. 7 is a second schematic structural view of the microfluidic chip according to the first embodiment;
fig. 8 is a schematic structural view of the operation platform in the first embodiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in detail below with reference to the accompanying drawings. However, it will be appreciated by those of ordinary skill in the art that numerous technical details are set forth in order to provide a better understanding of the present application in various embodiments of the present invention. However, the technical solution claimed in the present application can be implemented without these technical details and various changes and modifications based on the following embodiments.
The first embodiment of the present invention relates to a digital PCR instrument, as shown in fig. 1 to 8, which mainly comprises: the device comprises an operation platform 1, a first executing mechanism 2, a second executing mechanism 3, a positioning frame 4, a first air pressure generating mechanism 5, a second air pressure generating mechanism 6 and a main control device (not shown).
Wherein the operation platform 1 is used for placing the microfluidic chip 7, as shown in FIG. 4, the microfluidic chip 7 is provided with a fluid channel 71, and the fluid channel 71 has a channel inlet 72 and a channel outlet 73, the channel inlet 72 and the channel outlet 73 being respectively opened at the surface of the microfluidic chip 7, and the fluid channel 71 is embedded inside the microfluidic chip 7, as shown in fig. 6 and 7, the microfluidic chip 7 is provided with a first flexible valve body 74 communicating with the channel inlet 72, and a second flexible valve body 75 communicating with the channel outlet 73, the first flexible valve body 74 and the second flexible valve body 75 are made of a polymeric material and have excellent elastic deformability, the first flexible valve body 74 and the second flexible valve body 75 are bonded to the surface of the microfluidic chip 7 by an adhesive, this kind of connected mode is detachable connection, and first flexible valve body 74 and second flexible valve body 75 or microfluid chip 7 after the dismantlement can recycle after wasing.
The first actuator 2 is used for pressing the first flexible valve body 74, and the first flexible valve body 74 is pressed to close the channel inlet 72. Specifically, in the digital PCR instrument of the present embodiment, the first actuator 2 includes a first push pin 21 and a first telescoping device 22, wherein the first telescoping device 22 is a pneumatic or electric telescoping device, such as an air cylinder or an electric cylinder, the first telescoping device 22 is configured to drive the first push pin 21 to move, the channel inlet 72 is closed after the first push pin 21 pushes the first flexible valve body 74, and when the first push pin 21 is removed, the first flexible valve body 74 is restored to the original shape due to elastic deformation and continues to maintain the open state. The structure is relatively simple, the channel inlet 72 can be completely sealed, and the first telescopic device 22 can be operated remotely by matching with the main control equipment, so that the structure is more practical.
It should be noted that, in order to make the structure of the digital PCR instrument more compact, the operation platform 1 is provided with a first hollow portion 11, and the first pressing pin 21 passes through the first hollow portion 11, that is, the first pressing pin 21 reciprocates within the range of the operation platform 1. Preferably, the first flexible valve element 74 has a first inner passage 741 and a first pressing groove 742, the first pressing groove 742 extends in the direction of the first inner passage 741, and the first pressing groove 742 is used for the first pressing spike 21 to extend into. With less wall thickness of the pressed portion of first flexible valve body 74, first flexible valve body 74 is more easily deformed for closure of passage inlet 72. In this embodiment, the extending direction of the first pressing groove 742 is perpendicular to the direction of the first internal channel 741, the extending direction of the first pressing groove 742 is also consistent with the running direction of the first telescopic device 22, and the aperture from the notch to the bottom of the first pressing groove 742 is gradually reduced, which is beneficial to the insertion of the first pressing thimble 21, so that the allowable error range during assembly is large, and the assembly is easier. It will also be understood that the second actuator 3, which is used to squeeze the second flexible valve body 75, the second flexible valve body 75 is squeezed to close the channel outlet 73. In this embodiment, the second actuator 3 and the first actuator 2 are disposed in parallel and symmetrically, the operation direction is the same, and similar to the first actuator 2, the second actuator 3 includes a second pressing pin 31 and a second telescopic device 32, the second telescopic device 32 is configured to drive the second pressing pin 31 to move, and after the second pressing pin 31 presses the second flexible valve body 75, the channel outlet 73 is closed. The operating platform 1 is provided with a second hollow part, and the second pressing thimble 31 passes through the second hollow part, so that the structure of the digital PCR instrument is more compact. In this embodiment, the second hollow portion is communicated with the first hollow portion 11, or the second hollow portion and the first hollow portion 11 form a complete hollow structure on the operation platform 1, and certainly, if the second hollow portion and the first hollow portion 11 are not communicated with each other, an approximate effect can be achieved. The second flexible valve body 75 has a second internal passage 751 and a second pressing groove 752, the second pressing groove 752 extending in the direction of the second internal passage 751, and the second pressing groove 752 for the second pressing spike 31 to extend into. With a smaller wall thickness of the pressed portion of the second flexible valve body 75, the second flexible valve body 75 is more easily deformed for the closure of the passage inlet 72.
The positioning frame 4, as shown in fig. 5, is used for placing a first adapting pipe 41 and a second adapting pipe 42, wherein the first adapting pipe 41 is used for communicating with the first flexible valve body 74, and the second adapting pipe 42 is used for communicating with the second flexible valve body 75. In the present embodiment, the first adapter tube 41 and the second adapter tube 42 each have a sharp-nose shape, that is, have a sharp-nose shaped outlet, and the respective outlets are respectively inserted into the first internal passage 741 port of the first flexible valve body 74 and the first internal passage 741 port of the second flexible valve body 75, so as to facilitate connection. In addition, the positioning frame 4 further includes a first fixing portion for fixing the first flexible valve body 74, and a second fixing portion for fixing the second flexible valve body 75. As shown in fig. 1 to 4, the positioning frame 4 fixes the first adaptive pipe 41 and the second adaptive pipe 42, and also fixes the first flexible valve body 74 and the second flexible valve body 75, so that the connection between the first adaptive pipe 41 and the first flexible valve body 74 and the connection between the second adaptive pipe 42 and the second flexible valve body 75 are more stable, and the structure of the digital PCR instrument is more compact by using the same positioning frame 4. To further assist the operation of the two flexible valve bodies, as shown in fig. 5 and 7, the first flexible valve body 74 further has a first supporting slot 743, the first supporting slot 743 extends toward the first inner channel 741, and the positioning frame 4 further includes a first supporting thimble 43 for extending into the first supporting slot 743, and the first supporting thimble is disposed opposite to the first pressing thimble 21. To further reduce the thickness of the wall of the pressed portion of the first flexible valve body 74 and to provide a supporting force to the first pressing spike 21, so that the first flexible valve body 74 is more easily deformed to close the passage inlet 72. The positioning frame 4 and the first supporting thimble are of an integrated structure, and the aperture from the notch of the first supporting slot 743 to the bottom of the slot is gradually reduced, so that the first supporting thimble can extend into the slot. As will be understood, the second flexible valve body 75 also has a second support groove 753, the second support groove 753 extending in a direction toward the second internal passage 751; the positioning frame 4 further includes a second supporting thimble 44, which is used for extending into the second supporting groove 753, and the second supporting thimble is disposed opposite to the second pressing thimble 31. To further reduce the wall thickness of the pressed portion of the second flexible valve body 75 and to provide a supporting force to the second pressing spike 31, so that the second flexible valve body 75 is more easily deformed to close the passage inlet 72.
A first air pressure generating mechanism 5 for generating air pressure to the first adaptive pipe 41; the second air pressure generating means 6 generates air pressure to the second adaptive pipe 42. The first air pressure generating mechanism 5 and the second air pressure generating mechanism 6 can select a double positive pressure generating mechanism, a double negative pressure generating mechanism and a positive pressure-negative pressure generating mechanism as required, and can realize sample introduction of the microfluidic chip 7 through different operation flows. In addition, the negative pressure can also effectively save the consumption of precious reagents or samples.
The main control device is electrically connected with the first executing mechanism 2, the second executing mechanism 3, the first air pressure generating mechanism 5 and the second air pressure generating mechanism 6, and is used for controlling the first executing mechanism 2, the second executing mechanism 3, the first air pressure generating mechanism 5 and the second air pressure generating mechanism 6.
As can be seen from the above, the digital PCR instrument provided in this embodiment utilizes the microfluidic chip 7 having the channel inlet 72 and the channel outlet 73, the first flexible valve body 74 disposed at the channel inlet 72, the second flexible valve body 75 disposed at the channel outlet 73, the first actuator 2, the second actuator 3, the first air pressure generating mechanism 5, the second air pressure generating mechanism 6, and the main control device to implement sample injection of the microfluidic chip 7. Specifically, the first actuator 2 presses the first flexible valve body 74, and the second actuator 3 presses the second flexible valve body 75, so that the opening and closing of the channel inlet 72 and the channel outlet 73 can be selectively controlled, and the microfluidic chip 7 can be selectively closed, and then the first adapter tube 41 communicating with the first flexible valve body 74, the second adapter tube 42 communicating with the second flexible valve body 75, the first air pressure generating mechanism 5 for generating air pressure to the first adapter tube 41, the second air pressure generating mechanism 6 for generating air pressure to the second adapter tube 42 are combined, and the microfluidic sample is injected into the fluid channel 71 by using the positive pressure or the negative pressure generated by the first air pressure generating mechanism 5 and the second air pressure generating mechanism 6, and has a filling uniformity.
It should be noted that, in the digital PCR instrument of the present embodiment, as shown in fig. 8, the operation platform 1 is provided with a socket 12, and the socket 12 is matched with the shape of the microfluidic chip 7 for inserting the microfluidic chip 7 to fix the microfluidic chip 7. Further, the socket groove 12 includes a slide groove portion 13, and the slide groove portion 13 extends to the edge of the operation platform 1, so that the microfluidic chip 7 can be more easily mounted.
A second embodiment of the present invention relates to a digital PCR instrument. The second embodiment is an improvement of the first embodiment, and the main improvements are as follows: in the digital PCR instrument of the present embodiment, as also shown in fig. 1 to 3, the first air pressure generating mechanism 5 includes a first air pressure generating device 51 and a first joint 52 communicating with the first air pressure generating device 51; the digital PCR instrument further comprises a first movable plate 8, the first joint 52 is arranged on the first movable plate 8, the first movable plate 8 moves relative to the positioning frame 4 through the first stroke mechanism 9, the first stroke mechanism 9 can move towards the X-axis direction and the Z-axis direction in a coordinate system, the first stroke mechanism 9 can also be understood as a cylinder or an electric cylinder with at least two stroke directions to be combined and used for driving the first joint 52 to be communicated with the first adaptive pipe 41, so that the first joint 52 covers the pipe orifice of the first adaptive pipe 41, the structure improves the flexible operation performance of the digital PCR instrument, and the first adaptive pipe 41 can be conveniently replaced. Correspondingly, in the digital PCR instrument, the second air pressure generating mechanism 6 includes a second air pressure generating device and a second joint 62 communicated with the second air pressure generating device, the second air pressure generating device is the first air pressure generating device 51, of course, a second air pressure generating device may be additionally provided, the second joint 62 and the first joint 52 are arranged in parallel and symmetrically, and are also arranged on the first movable plate 8 and are driven by the first movable plate 8 to move, so that the flexible operation performance of the digital PCR instrument is also improved, and the second adaptive pipe 42 is convenient to replace. It should be understood by those skilled in the art that if the digital PCR instrument further includes a second movable plate, the second joint 62 is disposed on the second movable plate, and the second movable plate moves relative to the positioning frame 4 through the second stroke mechanism for driving the second joint 62 to communicate with the second adapting pipe 42, similar technical effects can be achieved.
It will be understood by those of ordinary skill in the art that the foregoing embodiments are specific examples for carrying out the invention, and that various changes in form and details may be made therein without departing from the spirit and scope of the invention in practice.
Claims (11)
1. A digital PCR instrument, comprising:
an operation platform for placing a microfluidic chip, the microfluidic chip having a fluid channel, and the fluid channel having a channel inlet and a channel outlet; the microfluidic chip is provided with a first flexible valve body communicated with the channel inlet and a second flexible valve body communicated with the channel outlet;
the first actuating mechanism is used for extruding the first flexible valve body, and the inlet of the channel is sealed after the first flexible valve body is extruded;
the second actuating mechanism is used for extruding the second flexible valve body, and the second flexible valve body seals the channel outlet after being extruded;
the positioning frame is used for placing a first adaptive pipe and a second adaptive pipe, wherein the first adaptive pipe is used for being communicated with the first flexible valve body, and the second adaptive pipe is used for being communicated with the second flexible valve body;
a first air pressure generating mechanism for generating air pressure to the first adaptive pipe;
a second air pressure generating mechanism for generating air pressure to the second adaptive pipe;
the main control equipment is used for respectively controlling the first executing mechanism, the second executing mechanism, the first air pressure generating mechanism and the second air pressure generating mechanism;
the locating rack includes:
the first fixing part is used for fixing the first flexible valve body;
the second fixing part is used for fixing the second flexible valve body;
the first air pressure generating mechanism comprises a first air pressure generating device and a first joint communicated with the first air pressure generating device;
the digital PCR instrument further comprises a first movable plate, the first joint is arranged on the first movable plate, and the first movable plate moves relative to the positioning frame through a first stroke mechanism and is used for driving the first joint to be communicated with the first adapter tube;
the second air pressure generating mechanism comprises a second air pressure generating device and a second joint communicated with the second air pressure generating device;
the digital PCR instrument further comprises a second movable plate, the second joint is arranged on the second movable plate, and the second movable plate moves relative to the positioning frame through a second stroke mechanism and is used for driving the second joint to be communicated with the second adapter tube.
2. The digital PCR instrument according to claim 1, wherein the operation platform is provided with a socket, the socket is matched with the shape of the microfluidic chip for inserting the microfluidic chip.
3. The digital PCR instrument of claim 2, wherein the socket slot includes a runner portion that extends to the platform edge.
4. The digital PCR instrument as claimed in claim 1, wherein the first actuator comprises a first pressing pin and a first expansion device, the first expansion device is configured to drive the first pressing pin to move, and the channel inlet is closed after the first pressing pin presses the first flexible valve body.
5. The digital PCR instrument of claim 4, wherein the operation platform is provided with a first hollow portion, and the first pressing thimble penetrates through the first hollow portion.
6. The digital PCR instrument of claim 5, wherein the first flexible valve body has a first internal passage and a first pressing groove, the first pressing groove extends toward the first internal passage, and the first pressing groove is used for the first pressing spike to extend into.
7. The digital PCR instrument of claim 6, wherein the first flexible valve body further has a first support groove extending toward the first internal passage;
the locating rack further comprises a first supporting ejector pin which is used for extending into the first supporting groove, and the first supporting ejector pin and the first pressing ejector pin are arranged oppositely.
8. The digital PCR instrument as claimed in claim 1, wherein the second actuator comprises a second push pin and a second expansion device, the second expansion device is configured to drive the second push pin to move, and the channel outlet is closed after the second push pin presses the second flexible valve body.
9. The digital PCR instrument of claim 8, wherein the operating platform is provided with a second hollow portion, and the second pressing pin penetrates through the second hollow portion.
10. The digital PCR instrument of claim 9, wherein the second flexible valve body has a second internal channel and a second pressing groove, the second pressing groove extends toward the second internal channel, and the second pressing groove is used for the second pressing spike to extend into.
11. The digital PCR instrument of claim 10, wherein the second flexible valve body further has a second support groove extending toward the second internal channel;
the positioning frame further comprises a second supporting thimble used for extending into the second supporting groove, and the second supporting thimble and the second pressing thimble are arranged oppositely.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710287845.7A CN107138193B (en) | 2017-04-27 | 2017-04-27 | Digital PCR instrument |
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|---|---|---|---|
| CN201710287845.7A CN107138193B (en) | 2017-04-27 | 2017-04-27 | Digital PCR instrument |
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| CN107138193A CN107138193A (en) | 2017-09-08 |
| CN107138193B true CN107138193B (en) | 2020-02-25 |
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| CN112940928A (en) * | 2021-02-19 | 2021-06-11 | 清华大学 | Fully-integrated pathogen nucleic acid analyzer |
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