CN107132102A - A kind of improved pig parasitic disease excrement detecting method - Google Patents
A kind of improved pig parasitic disease excrement detecting method Download PDFInfo
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- CN107132102A CN107132102A CN201710465973.6A CN201710465973A CN107132102A CN 107132102 A CN107132102 A CN 107132102A CN 201710465973 A CN201710465973 A CN 201710465973A CN 107132102 A CN107132102 A CN 107132102A
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- 238000000034 method Methods 0.000 title claims abstract description 46
- 208000030852 Parasitic disease Diseases 0.000 title claims abstract description 10
- 241000282898 Sus scrofa Species 0.000 claims abstract description 45
- 239000007788 liquid Substances 0.000 claims abstract description 31
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000004202 carbamide Substances 0.000 claims abstract description 27
- 238000001514 detection method Methods 0.000 claims abstract description 13
- 238000000386 microscopy Methods 0.000 claims abstract description 13
- 239000004094 surface-active agent Substances 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 8
- 239000002893 slag Substances 0.000 claims abstract description 7
- 239000000344 soap Substances 0.000 claims description 25
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical group [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 241000224483 Coccidia Species 0.000 claims description 6
- 206010023076 Isosporiasis Diseases 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 125000001931 aliphatic group Chemical group 0.000 claims description 2
- 201000008167 cystoisosporiasis Diseases 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 201000010099 disease Diseases 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract description 16
- 229920006395 saturated elastomer Polymers 0.000 abstract description 13
- 235000002639 sodium chloride Nutrition 0.000 abstract description 13
- 239000011780 sodium chloride Substances 0.000 abstract description 11
- 102000004169 proteins and genes Human genes 0.000 abstract description 10
- 108090000623 proteins and genes Proteins 0.000 abstract description 10
- 230000000007 visual effect Effects 0.000 abstract description 9
- 238000005259 measurement Methods 0.000 abstract description 8
- 238000012795 verification Methods 0.000 abstract description 8
- 238000004925 denaturation Methods 0.000 abstract description 3
- 230000036425 denaturation Effects 0.000 abstract description 3
- 230000002209 hydrophobic effect Effects 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 230000007547 defect Effects 0.000 abstract description 2
- 238000004945 emulsification Methods 0.000 abstract description 2
- 150000003384 small molecules Chemical class 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 239000000843 powder Substances 0.000 description 16
- 239000000243 solution Substances 0.000 description 11
- 238000005188 flotation Methods 0.000 description 8
- 239000011521 glass Substances 0.000 description 7
- 239000002775 capsule Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 238000007689 inspection Methods 0.000 description 6
- 241000209630 Cystoisospora suis Species 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 210000003250 oocyst Anatomy 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 244000045947 parasite Species 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000223924 Eimeria Species 0.000 description 1
- 241000567229 Isospora Species 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- -1 fatty acids salt Chemical class 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 210000004215 spore Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
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Abstract
The invention discloses a kind of improved pig parasitic disease excrement detecting method.First toward adding urea liquid in swine excrement, while add surfactant, stir and evenly mix and filter and stood after thick excrement slag, stood again after defrother is added dropwise, take surface solution progress microscopy.Instant invention overcomes the defect of traditional saturated aqueous common salt floating method, the principle of protein denaturation is made by using urea, the protein in detectable substance is removed；Add a certain amount of surfactant, utilize the hydrophilic and oleophobic base and oleophyllie hydrophobic base of surfactant, make fat emulsification in excrement into small molecule fat drips, so as to remove influence of the fat to detection, defrother is added simultaneously and removes the bubble produced when using surfactant by stirring so as to improve the microscopy visual field, improves verification and measurement ratio；The present invention is simple, convenient, and verification and measurement ratio is high, and raw material is easy to get, is well suited for promoting the use of in actual clinical.
Description
Technical field
The invention belongs to animal diseases diagnosis technical field.Examined more particularly, to a kind of improved pig parasitic disease excrement
Method.
Background technology
Pig global-worm illness is to parasitize the Eimeria coccidia of swine intestinal epithelium cells and wait spore to belong to coccidia and cause, to suffer from diarrhoea and
Become thin as cardinal symptom.The Zhu Junneng infected pigs coccidia of different cultivars and growth phase, wherein nursery-age pig is to sorospheres such as pigs
Worm（Isospora suis）Neurological susceptibility highest, clinic often there is watery diarrhea, slow-growing or even death.Isospora suis
It is popular extensive, Isospora suis can be detected on the pig farm of Canada 70%, at home, Sichuan infection rate is even as high as 87.50%.
Pig global-worm illness brings serious economic loss to aquaculture.
The principle of early treatment is found based on morning, checkout and diagnosis pig global-worm illness can reduce very big economic loss in time.But
It is that traditional excrement detecting method has many problems, particularly suckling pig global-worm illness when detecting global-worm illness, due to lactation porkling
Digestive system does not develop perfect, when infecting Isospora suis, causes enteron aisle to be damaged, indigestion, exists largely not in excrement
The impurity such as protein, the fat of digestion, in the flotation process with saturated aqueous common salt floating method, because high salt concentration makes protein
Generation salting-out phenomenon, makes Proteins float in liquid table, along with liquid table adrift substantial amounts of fat, microscopy visual field when causing microscopy
Unintelligible, verification and measurement ratio is low, detection difficult, and detection false negative rate is increased.
The content of the invention
The technical problems to be solved by the invention are to overcome pig global-worm illness excrement detecting method verification and measurement ratio is low in the prior art to lack
Fall into and deficiency is there is provided a kind of pig parasitic disease excrement detecting method, can be floated in hyperbaric solution using pig parasite egg/egg capsule
Floating principle, is replaced as the floating method of solute from urea and shows clinically usual saturated aqueous common salt floating method, saturation urea
The proportion of solution is suitable with saturated aqueous common salt, and can make protein denaturation, while using neat soap（Principal component is received for aliphatic acid）Remove
Fat is removed, then the air bubble problem of detection process is solved with silicate defrother, so as to improve the microscopy visual field, detection is improved
Rate, the degree of accuracy is high.Methods described is simple, convenient, and verification and measurement ratio is high, and raw material is easy to get, and being well suited for promoting in actual clinical makes
With.
It is an object of the invention to provide a kind of improved pig parasitic disease excrement detecting method.
The above-mentioned purpose of the present invention is to give realization by the following technical programs：
A kind of improved pig parasitic disease excrement detecting method, methods described is to add urea liquid into swine excrement, while adding table
Face activating agent, stirs and evenly mixs and filters standing after thick excrement slag, stood again after defrother is added dropwise, take surface solution to carry out microscopy.
The principle that the present invention can be floated using pig parasite egg/egg capsule in hyperbaric solution, from urea as molten
The floating method substitution of matter shows clinically usual saturated aqueous common salt floating method, proportion and the saturated common salt aqueous phase of saturation urea liquid
When, and protein denaturation can be made, the protein in detectable substance can be removed；Surfactant is added simultaneously, utilizes surface-active
The hydrophilic and oleophobic base and oleophyllie hydrophobic base of agent, make the fat emulsification in excrement into small molecule fat drips, so as to remove fat to detection
Influence；Due to when surface reactive material removes fatty impurity, certain bubble can be produced in whipping process, it is difficult to dissipate, so
Bubble can be removed by adding a certain amount of defrother, make the microscopy visual field apparent, improve verification and measurement ratio.
Preferably, methods described is to add urea liquid into swine excrement, while adding surfactant, is stirred and evenly mixed simultaneously
Filter 1~2min of standing after thick excrement slag（It is preferred that 1min）, then 1~2 drop defrother is added dropwise（It is preferred that 1 drop）, stand 10 min~15
min（It is preferred that 15min）After take liquid table solution to be detected.
Preferably, the thick excrement slag of filtering is to be sieved through the thick excrement slag of filter with 60 mesh copper.
Preferably, the mass volume ratio of the swine excrement and urea liquid and surfactant is 1g：6~10ml：0.1
~0.3g.
Preferably, the mass fraction of the urea liquid is 50%.
Preferably, the surfactant is soap.
It is highly preferred that the soap is neat soap；Before use, can be by neat soap grind into powder, to improve neat soap molten
Diffusion velocity in liquid.
Preferably, the defrother is silicate defrother.
Silicate in the silicate defrother contains hydrophobic active substances, and adsorbable surface-active substance makes bubble surface
Tension force becomes causes it to rupture greatly, the problem of solution bubble that can be quickly.
Preferably, the pig parasitic disease is pig global-worm illness.
Because the nutrition of suckling pig places one's entire reliance upon breast milk, the sucking pig excrement composition after infection Isospora suis contains not
The materials such as complete protein, fat are absorbed, the next certain difficulty of the detection band to suckling pig coccidian oocyst, and institute of the present invention
State method and solve above mentioned problem just, therefore the method for the invention is particularly suitable for use in the detection of coccidiasis in piglets on some farms.
Preferably, the pig global-worm illness is the pig isosporiasis.
As a kind of selectable embodiment, the method for the invention is used for concrete operations when coccidiasis in piglets on some farms excrement is examined
Method comprises the following steps：
S1. neat soap is dried, smashed with mortar；Urea liquid is configured to the floating solution that mass fraction is 50%.
S2. take 3 g piglets excrement in 100 ml beakers, first add 10 ml urea liquids, while 0.1 g neat soap powder is added,
Stir, add 20 ml urea liquids, stir evenly again, filter is sieved through with 60 mesh copper, stand and a drop is added dropwise after 1 min
Silicate defrother, liquid level is gently agitated for glass bar, stands 15 min.
S3. with taking pendular ring to take liquid table solution 2~3 to drip in slide same position, observe under an optical microscope, detect ovum
Capsule number.
Compared with prior art, the invention has the advantages that：
（1）Instant invention overcomes the defect of traditional saturated aqueous common salt floating method, by using urea liquid and surfactant, have
Effect eliminates protein and fat in swine excrement, removes bubble by adding defrother, so as to improve the microscopy visual field, improves
Verification and measurement ratio.
（2）The present invention is simple, convenient, and verification and measurement ratio is high, and raw material is easy to get, is well suited for promoting the use of in actual clinical.
Brief description of the drawings
Fig. 1 is each group microscopy result of embodiment 1, and a, b, c, d represent 1 respectively, 2,3,4 groups of result of the test.
Fig. 2 for the neat soap powder optimised quantity of embodiment 2 determination experiment excrement inspection cup in liquid top layer glossy comparison diagram, a, b, c, d,
E, f represent the 1st respectively, 2,3,4,5,6 groups of result of the test.
Fig. 3 is the determination experiment microscopy comparison diagram of neat soap powder optimised quantity, a, b, c, d, e, f represent the 1st respectively, 2,3,4,5,
6 groups of result of the test.
Fig. 4 is the line chart of the determination experiment of the present invention optimal flotation time.
Embodiment
The present invention is further illustrated below in conjunction with Figure of description and specific embodiment, but embodiment is not to the present invention
Limit in any form.Unless stated otherwise, the reagent of the invention used, method and apparatus routinely try for the art
Agent, method and apparatus.
Unless stated otherwise, following examples agents useful for same and material are purchased in market.
A kind of improved pig global-worm illness excrement detecting method of embodiment 1
1st, take the thick piglet excrement of 12 g greases fully to mix, divide four parts equally and load four excrement inspection cups, be organized as 1,2,3,4
Group.
The 1st group of ml saturated common salt aqueous solution of addition 30；
The 2nd group of ml urea liquid of addition 30（50%）；
The 3rd group of ml urea liquid of addition 30（50%）Add a little neat soap powder solution simultaneously；
4th group on the basis of the 3rd group float one minute after add one drip defrother；
Solution takes 2~3 ring upper liquids in slide same position mirror after floating certain time with pendular ring is taken in above-mentioned each group
Inspection, contrasts the definition in each visual field.
2nd, result
As a result as shown in figure 1, the 4th group（Urea+neat soap powder+silicate defrother）Effect is best, among actual production
The detection of pig global-worm illness.
The measure of the neat soap powder optimised quantity of the pig global-worm illness excrement of embodiment 2 inspection
1st, in being surface reactive material, whipping process due to neat soap major components fatty acids salt, certain bubble can be produced, works as addition
Neat soap powder amount it is more, the bubble of generation is more, influence detection, when bubble is excessive, and defrother de-bubble also seems that some are difficult, and
And add to be also excessively a kind of waste, accordingly, it would be desirable to be determined to the addition of neat soap powder, experimental procedure is as follows：
（1）Take the thick piglet excrement of 18 g greases first fully to mix, be bisected into six parts and be added in 6 100 ml excrement inspection cups,
It is divided into 1~6 group, the 1st group of addition 30ml saturated aqueous common salt, remaining each group is separately added into 30 ml urea liquids（50%）, fully stir
Mix to excrement and be completely dissolved, filter.
（2）The gradient of neat soap powder amount is set：0 g, 0.1 g, 0.3 g, 0.5 g, 0.7 g, are separately added into the 2nd, 3,4,5,6
In group beaker, the 1st group is that saturated aqueous common salt group is compared, and is sufficiently stirred for.
（3）The oil layer observed in cup（As shown in Figure 2）, while with taking pendular ring to take ring upper liquid in slide, microscopy,
Contrast the definition in each visual field.
2nd, result
Repeatedly test, show the 3rd group of content for optimal neat soap powder amount, liquid top layer is glossy most light in beaker（Such as Fig. 2 c）
And the observation of the microscopy visual field is most clear（Such as Fig. 3 c）.
The measure of the optimal flotation time of the pig global-worm illness excrement of embodiment 3 inspection
1st, due to urea liquid（50%）Proportion be 1.14, be different from and the proportion 1.20 of saturated aqueous common salt, therefore the flotation time
Can not be with reference to saturated aqueous common salt, it is necessary to redefine, experimental procedure is as follows：
（1）It is 0 min, 5 min, 10 min, 15 min, 20 min, 25 min to set flotation time gradient.
（2）Take in 6 100 ml beakers, each beaker and add 30 ml urea liquids（50%）.
（3）10000 coccidian oocysts are added in each beaker, while being sufficiently stirred for and starting timing.
（4）Since 0 min, 5 min are often crossed respectively with taking pendular ring in the middle of the cup and nearby take a ring to be added to load glass
The same position of piece, adds cover glass, and the quantity of 5 rows or 5 column count coccidian oocysts is taken at random, to determine during optimal floating
Between.
2nd, result
As shown in table 1, egg sac number is with the change line chart of flotation time as shown in figure 4, from above-mentioned number for three repetition experimental results
Find out according to that can draw：When the flotation time is 10 min~15 min, you can observed.
The egg sac number of table 1 with the flotation time variation relation
The application of the improved pig global-worm illness excrement detecting method of embodiment 4
1st, the application of improved pig global-worm illness excrement detecting method
（1）The thick piglet excrement of 30 g greases is first fully mixed, divides equally and is fitted into six 100 ml beakers, divide two groups equally
（First group, second group）.Every group of three parallel group # are all 1~3.
（2）First group every glass plus 30 ml satisfy salt solution, second group every glass 30 ml urea liquids（50%）0.1 g is added simultaneously
Neat soap powder.And 10,000 egg capsules are added in every glass.Mix, be sieved through and filtered with 60 mesh copper, Jia one to second group every glass after 1 min drips
Defrother, is gently mixed liquid level.Every group takes three visuals field meter egg sac numbers, each beaker with taking pendular ring to take three drops, in often dripping at random
Egg capsule count, with three drop average counting numbers.
2nd, result
As a result as shown in table 2, second group（Urea+neat soap powder+silicate defrother floating method group）The egg capsule amount detected is obvious
More than saturated aqueous common salt floating method.
Table 2
The clinical practice of the improved pig global-worm illness excrement detecting method of embodiment 5
1st, the clinical practice of improved pig global-worm illness excrement detecting method
（1）Three pig farm piglet excrement are taken, each 20 parts of samples fully mix every part of sample, stand-by.
（2）Prepare 120 100 ml glasss, divide two groups equally.First group is set to saturated aqueous common salt group, and second group is set to urine
Element+neat soap powder+defrother group.Divide every part of sample equally two parts of progress two groups of experiments, and correspond detection and compare, Ran Houxiang
First group of ml saturated aqueous common salt of addition 30, the second group of ml urea of addition 30+neat soap powder solution is fully mixed, and is sieved with 60 mesh copper
Jia one to second group every glass after filtering, 1 min and drip defrother, be gently mixed liquid level.
2nd, result
Testing result is as shown in table 3, second group（Neat soap powder+urea+defrother group）Three pig farm isospora positive rate difference
For：35%, 25%, 33%, and saturated common salt water component is not：5%, 5%, 10%.Show second group（That is the present invention program）Detection
Positive rate is substantially better than first group.
Table 3
Claims (10)
1. a boar parasitic disease excrement detecting method, it is characterised in that add urea liquid into swine excrement, lives while adding surface
Property agent, stir and evenly mix and filter after thick excrement slag stand, be added dropwise defrother after stand again, take surface solution carry out microscopy.
2. excrement detecting method according to claim 1, it is characterised in that described to stir and evenly mix and filter what is stood after thick excrement slag
Time is 1~2min, and it is the min of 10 min~15 that time for standing again, which is added dropwise after defrother,.
3. excrement detecting method according to claim 1, it is characterised in that the swine excrement and urea liquid and surface-active
The mass volume ratio of agent is 1g：6~10ml：0.1~0.3g, the consumption of the defrother is 1~2 drop.
4. the excrement detecting method according to any one of claims 1 to 3, it is characterised in that the mass fraction of the urea liquid
For 40~60%.
5. the excrement detecting method according to any one of claims 1 to 3, it is characterised in that the surfactant is aliphatic acid
Salt.
6. the excrement detecting method according to any one of claims 1 to 3, it is characterised in that the defrother is silicate de-bubble
Agent.
7. excrement detecting method according to claim 5, it is characterised in that the soap is neat soap.
8. the excrement detecting method according to any one of claim 1~7, it is characterised in that the pig parasitic disease is pig coccidia
Disease.
9. application of the excrement detecting method in the detection of pig global-worm illness described in any one of claim 1~8.
10. excrement detecting method according to claim 9, it is characterised in that the pig global-worm illness is the pig isosporiasis.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652315A (en) * | 2018-11-23 | 2019-04-19 | 佛山市正典生物技术有限公司 | A method of improving pig coccidian oocyst separative efficiency and cleanliness |
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