CN107121771A - Adaptive optics focuses on interference compensation method and system - Google Patents
Adaptive optics focuses on interference compensation method and system Download PDFInfo
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- CN107121771A CN107121771A CN201710322144.2A CN201710322144A CN107121771A CN 107121771 A CN107121771 A CN 107121771A CN 201710322144 A CN201710322144 A CN 201710322144A CN 107121771 A CN107121771 A CN 107121771A
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
- G02B26/06—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the phase of light
Abstract
Interference compensation method and system are focused on the invention discloses a kind of adaptive optics.The present invention first passes through the fluorescence inspired in sample and received by photomultiplier, and now focusing center's light intensity is threshold value to note；DMD is placed in after object lens in the conjugate planes in pupil face, and to its subregion, area's incident beam is reflexed to side light path by each subregion successively, makes to return to positive side light path after its phase compensation, while keeping other subregion light beams constant；Focusing center's light intensity after the change of photomultiplier record partitioning beam phase；Mark light intensity to be more than or equal to the subregion of threshold value, obtain compensation subregion；Light beam is focused on again after DMD loading compensation subregion, and the stronger hot spot of central light strength is formed in sample.The present invention can quickly improve the quality of scattering medium internal focus hot spot from principle of optical interference, be that light science of heredity and live body deep layer high-resolution optical micro-imaging technique provide new approaches.
Description
Technical field
The invention belongs to light science of heredity and optical microphotograph imaging field, a kind of more particularly to adaptive optics focuses on interference
Compensation method and system, and be imaged applied to Noninvasive light science of heredity light stimulus of new generation and live body deep penetration optical microphotograph.
Background technology
In biomedical optical community, optical scattering is the principal element for restricting quality of optical imaging.Most deep tissues
The optical technology (for example, laser confocal imaging, Two Photon Fluorescence and optical coherence tomography scanning) of imaging is mainly using non-
Scattered photon (i.e. trajectory photon) is imaged.With depth increase, exponentially formula decays the quantity of trajectory photon, therefore by optical focus
Scope has been limited in 1mm depth.
Light genetics technology needs to carry out light stimulus to specific neuron, to study its neural circuitry mechanism.But pass
Damage of the embedded fibers formula light science of heredity of system to living body biological is serious, is unfavorable for studying for a long period of time.Previously apply in astronomy
Adaptive optical technique, for realize the light stimulus of deep layer biological tissue with imaging provide new technical support.
Existing non-intrusion type adaptive optical genetics technology is the fine phase correction based on adaptive optics, by inciting somebody to action
Spatial light modulator is divided into some subregions, changes the light beam additive phase in subregion at equal intervals successively, detects its optimal optics and gather
Burnt phase.Each subregion loop iteration successively, so as to obtain correction of a final proof phase, phase compensation is carried out to incident beam, so that
Its distorted phase is corrected, good optical is formed and focuses on.
But above fine phase correction needs to consume the substantial amounts of time, in order to obtain preferably optical correction, it is necessary to
Divide more subregions and thinner phase intervals.Because the image refresh rate of spatial light modulator is relatively low, cause optical correction
The plenty of time is consumed, is unfavorable for carrying out the stimulation of Real-Time Optical science of heredity and Real-time High Resolution rate optical imagery, restriction in living body biological
The popularization of adaptive optical genetic technique and microtechnic.
The content of the invention
In order to solve problem present in background technology, present invention aims at utilize higher digital micro- of image refresh rate
Mirror device solves the problem of traditional adaptive optical science of heredity spatial light modulator is time-consuming longer.From principle of optical interference,
Light beam is divided into some regions, and detects interference effect of each subregion inner light beam to focusing center.By retaining to focusing center
The light beam in region that interference is mutually grown, while interfering focusing center the light beam additional phase error π in the region of cancellation, expires it
Foot interference phase elongate member is simultaneously introduced back into light path.By the interference that script is played to negative effect to focal beam spot center
The light beam of cancellation switchs to interfere mutually long light beam so that light beam is fully used, so as to improve the matter of optical focus
Amount, shortens the correction time of adaptive optical stimulation.
To achieve these goals, technical scheme comprises the following steps：
First, a kind of adaptive optics focuses on interference compensation method：
1) focal plane of object lens does not place laboratory sample, and carrying out light beam with the DMD without loading subregion gathers
Jiao, ideal focusing hot spot is obtained in the focal plane of object lens, records the focusing center position O of ideal focusing hot spotf；
2) laboratory sample is placed in the focal plane of object lens, light intensity spy is carried out with the DMD without loading subregion
Survey, record obtains focusing center position OfLight intensity value, be used as Intensity threshold Ith；
3) reflecting surface of DMD is divided into polylith region, using each region successively as target area, to mesh
The reflected beams for marking region carry out phase compensation, and traversal regional carries out light intensity spy with the DMD of loading subregion
Survey, obtain the focusing center position O that regional corresponding record is arrivedfA series of light intensity values at place；
4) by each light intensity value and Intensity threshold IthIt is compared, and processing obtains judged result in the following ways：
If light intensity value is less than Intensity threshold Ith, then the corresponding region of the light intensity value is to be not required to phase compensation region；
If light intensity value is more than or equal to Intensity threshold Ith, then the corresponding region of the light intensity value is to need phase compensation region；
5) subregion will be adjusted according to judged result and will be loaded on DMD and carries out light intensity detection, in laboratory sample
The interior final optics focus-compensating hot spot formed after interference compensation, is O in focusing center positionfPlace inspires stronger fluorescence.
The step 1) in light beam focus on be specifically：Laser launches light beam, after collimator and extender, micro- in numeral
Reflect, then focused on by object lens on mirror device.
The step 3) in signified phase compensation specifically refer to, it is π's that a numerical value is added in the former phase of light beam
Phase difference.
The step 2), 3) and 5) in light intensity detection be specifically：Laser launches light beam, after collimator and extender,
Reflect, then focused on by object lens on sample on DMD, sample carries fluorescent material, is produced in sample abnormal
Become scattering hot spot and inspire fluorescence, the fluorescence inspired is swept after lens focus with photomultiplier and galvanometer again
Retouch detection and obtain the fluoroscopic image that scattering hot spot is inspired, record focusing center position OfLight intensity value.
The step 3) when being carried out light intensity detection with the DMD of loading subregion and is specifically referred to light intensity detection, it is digital
Itself is received the reflected beams produced after incident beam and adjusts reflection angle by the target area on micro mirror element, makes the reflected beams
To side light path return after phase compensation and do not adjust in the reflected light path before reflection angle, while in addition to target area
Other regions keep the reflected beams reflection angle and the step 2) light intensity detection when the reflected beams reflection angle it is identical.
The step 5) specifically referred to according to judged result compensation subregion：During by DMD, phase is needed after judgement
Itself is received the reflected beams produced after incident beam and adjusts reflection angle by position compensatory zone, makes the reflected beams to side light path
Returned after phase compensation and do not adjust in the reflected light path before reflection angle, while being not required to phase compensation region guarantor after judging
Hold the reflection angle and the step 2 of the reflected beams) light intensity detection when the reflected beams reflection angle it is identical.
Described fluorescent material includes fluorescin, fluorescence beads or fluorescent dye.
The laboratory sample is but is not limited to vivo biological tissue, tissues in vitro, agar block containing bead etc..
The step 3) in the reflecting surface of DMD be divided into polylith region specifically referred to DMD
Micromirror pixels member with n × n mode uniform segmentations, so that the incident beam after collimator and extender is divided into corresponding n × n light beam
Unit.
2nd, a kind of adaptive optics focuses on interference compensation system：
System includes laser, beam collimation and expands module, DMD, phase compensation block, beam splitter, shrink beam
Module, scan module, dichroscope, microcobjective, laboratory sample and light intensity detection module.Beam collimation expands module arrangement and existed
After laser, laser launches collimated expand after module parallel expands union space filtering of light beam and incides digital micromirror device
Phase compensation block is equipped with front of part, DMD side exit end, DMD positive side exit end is equipped with beam splitting
Device and shrink beam module, phase compensation block are located at the side of beam splitter, and the side the reflected beams of DMD are through phase
It is re-reflected into after compensating module in beam splitter, the positive side the reflected beams of DMD are directly reflexed in beam splitter；Shrink beam
Dichroscope is provided with front of module；Light beam is focused on after being reflected through dichroscope through over-scanning module into microcobjective, tests sample
Grade is on microcobjective focal plane；The fluorescence inspired in laboratory sample after microcobjective and scan module through two to
Look mirror is received by light intensity detection module and carries out light intensity detection.
Described beam collimation expands module and expands module lens, light beam spatial filter and rear light including preceding beam collimation
Beam collimator and extender module lens；Preceding beam collimation expands module lens, light beam spatial filter and rear beam collimation and expands module
Lens are arranged in parallel in the front at laser emitting end successively, and premenstrual beam collimation expands module lens, light to incident beam successively
Beam spatial filter and rear beam collimation incide DMD after expanding that module lens are parallel and expanding.
Described phase compensation block includes but is not limited to optical delay line, spatial light modulator, phase delay chip etc..
Described shrink beam module includes preceding shrink beam module lens and rear shrink beam module lens；Preceding shrink beam module lens and retraction
Beam module lens are arranged in parallel in the front side of beam splitter successively, and premenstrual shrink beam module is saturating successively for the light beam of DMD reflection
The side of dichroscope is incided after mirror shrink beam parallel with rear shrink beam module lens.
Described scan module shakes including preceding scanning galvanometer, preceding light beam collimation lens, rear light beam collimation lens, rear scanning
Mirror, preceding scan module lens and rear scan module lens；Preceding scanning galvanometer, preceding light beam collimation lens, rear light beam collimation lens, after
Scanning galvanometer, preceding scan module lens and rear scan module lens are sequentially arranged in the front side of dichroscope, dichroscope reflection
Light beam premenstrual scanning galvanometer, preceding light beam collimation lens, rear light beam collimation lens, rear scanning galvanometer, preceding scan module are saturating successively
Microcobjective is incided after mirror and rear scan module lens.
Described light intensity detection module includes optical filter, condenser lens, spatial filter and photomultiplier；Optics
Wave filter, condenser lens, spatial filter and photomultiplier are sequentially arranged in the rear side of dichroscope, what laboratory sample was sent
Fluorescence successively through microcobjective, after scan module lens, preceding scan module lens, after scanning galvanometer, after light beam collimation lens, preceding
Enter photoelectricity times after light beam collimation lens, preceding scanning galvanometer, dichroscope, optical filter, condenser lens and spatial filter
Increase pipe and carry out light intensity detection.
The beneficial effects of the invention are as follows：
The present invention realizes easy adaptive optics focus-compensating using DMD, utilizes DMD
Quick image refresh rate, overcomes conventional utilization space optical modulator and carries out the problem of speed is slow during serial phasing,
Improve the speed of optical focus and optical stimulation.
The present invention is based on optical interference image-forming principle, judges there is interference cancellation to focal beam spot by DMD
The subregion light beam of effect, and by the phase difference that added value is π in its phase, it is met the condition that interference is mutually grown, so that
The light intensity for obtaining focusing center is obviously improved, and improves the quality of strong scattering medium internal opticses focusing.
The present invention carries out adaptive optics correction using DMD, and has a variety of easy modes to realize that phase is mended
Repay, reduce experimental cost, more conducively application of the method and system in research experiment.
And the present invention easily can be combined with existing various micro-imaging techniques, contribute to non-embedded fibers formula light
Science of heredity light stimulus technology develops, and realizes the synchronous light stimulus in brain depths and micro-imaging, promotes brain science progress.
Brief description of the drawings
Fig. 1 is the structural representation of present system；
Fig. 2 is the scattering focal beam spot image of nil micro mirror element timing in embodiment；
Gained light intensity value and the comparison figure of threshold value after Fig. 3 is compensated successively for DMD subregion in embodiment；
Fig. 4 needs the segmented phase distribution map of compensation after judging for DMD in embodiment；
Fig. 5 is carries out the focal beam spot image after phase compensation in embodiment to the subregion of compensation in need.
Embodiment
Following adaptive optics focus-compensating embodiment can be with the more detailed description present invention, but does not limit in any form
The present invention.
Embodiments of the invention and its detailed process are as follows：
As shown in figure 1, present system, which includes laser 1, preceding beam collimation, expands module lens 2, light beam space filtering
It is saturating that device 3 and rear beam collimation expand module lens 4, DMD 5, phase compensation block 6, beam splitter 7, preceding shrink beam module
Mirror 8 and rear shrink beam module lens 9, dichroscope 10, preceding scanning galvanometer 11, preceding light beam collimation lens 12, rear light beam collimation lens
13rd, rear scanning galvanometer 14, preceding scan module lens 15 and rear scan module lens 16, microcobjective 17, laboratory sample 18, optics
Wave filter 19, condenser lens 20, spatial filter 21 and photomultiplier 22.
(1) beam collimation expands module lens 2, the and of light beam spatial filter 3 before the light beam that laser 1 is sent is sequentially passed through
Beam collimation is expanded after module lens 4 are expanded afterwards, is irradiated on DMD 5.In DMD 5 without loading
Image and when being not loaded with laboratory sample 18, it is saturating that light beam is reflected through beam splitter 7, preceding shrink beam module lens 8 and rear shrink beam module
Mirror 9, the side for being irradiated to dichroscope 10 is reflected, the reflected beams by preceding scanning galvanometer 11, preceding light beam collimation lens 12, after
Enter microcobjective after light beam collimation lens 13, rear scanning galvanometer 14, preceding scan module lens 15 and rear scan module lens 16
17, and preferable focal beam spot is formed in focal plane, note focal beam spot center is O in the position of focal planef。
(2) loading experiment sample 18, when DMD 5 is without load image, the light beam that laser 1 is sent is sequentially passed through
Preceding beam collimation expands module lens 2, light beam spatial filter 3 and rear beam collimation and expanded after module lens 4 are expanded, and shines
It is mapped on DMD 5, light beam is reflected through beam splitter 7, preceding shrink beam module lens 8 and rear shrink beam module lens 9, shines
The side for being mapped to dichroscope 10 is reflected, the reflected beams by preceding scanning galvanometer 11, preceding light beam collimation lens 12, after light beam it is accurate
Into microcobjective 17 after straight lens 13, rear scanning galvanometer 14, preceding scan module lens 15 and rear scan module lens 16, and
The scattering focal beam spot of focal plane formation distortion in laboratory sample 18, and inspire fluorescence.
(3) fluorescence is emitted into microcobjective 17 out of laboratory sample 18, then in turn through rear scan module lens 16,
Preceding scan module lens 15, rear scanning galvanometer 14, rear light beam collimation lens 13, preceding light beam collimation lens 12, preceding scanning galvanometer 11,
Enter photomultiplier 22 after dichroscope 10, optical filter 19, condenser lens 20 and spatial filter 21 and carry out light intensity spy
Survey, the scattering focal beam spot image of nil micro mirror element timing is obtained by scanning, as shown in Figure 2.Remember now OfCorrespondence
The light intensity value I of positionthFor Intensity threshold.Because light intensity point of maximum intensity is offset in this specific implementation case, OfThe light of correspondence position
Intensity values IthFor 15.76.
(4) the micromirror pixels member of DMD is uniformly divided into 32 × 32 modes in this specific implementation case
1024 regions, so that the incident beam after collimator and extender is divided into corresponding 1024 beam units.It is micro- in one subregion
Mirror is deflected to same direction simultaneously, and area's light beam is reflexed to the phase compensation block 6 of side light path, make its phase change π it
After return to former reflected light path, at the same time, micro mirror in other subregions keeps constant so that its light beam formation optical focus, i.e.,
The light beam in a subregion is only removed, and keeps the light beam of remaining 1023 subregion constant, by photomultiplier record in OfPlace
Fluorescent intensity value.All subregions are repeated in above-mentioned steps until recording the corresponding light intensity value of all subregions.
(5) by 1024 light intensity values obtained by step (4) and Intensity threshold I obtained by step (3)thCompare, obtain such as Fig. 3 institutes
Show beam intensity ratio relatively figure.Keep light intensity value constant less than the subregion of threshold value and be more than or equal to the Labelling Regions of threshold value to mend by light intensity value
Subregion is repaid, sectional image is compensated.Gained light intensity after DMD subregion is compensated successively in this specific implementation case
The comparison figure of value and threshold value is as shown in Figure 3；The segmented phase distribution map of required compensation is as shown in Figure 4.
(6) compensation sectional image is loaded on DMD 5, the premenstrual beam collimation of incident beam expands module
Lens 2, light beam spatial filter 3 and rear beam collimation are expanded after module lens 4 are expanded, and are irradiated to DMD 5
Above, it is necessary to which the corresponding light beam of subregion of compensation is reflected onto in the phase compensation block 6 of side light path, so as to occur π phase
Difference changes, and return in beam splitter 7 together with light beam corresponding with retained subregion by preceding shrink beam module lens 8 and after shrink beam
Module lens 9, the side for being irradiated to dichroscope 10 is reflected, and the reflected beams are saturating by preceding scanning galvanometer 11, preceding beam collimation
Enter after mirror 12, rear light beam collimation lens 13, rear scanning galvanometer 14, preceding scan module lens 15 and rear scan module lens 16 aobvious
Speck mirror 17, and focal plane forms final optical focus compensation hot spot in laboratory sample 18, and excite stronger fluorescence.
Phase compensation block used is optical delay line in this specific implementation case.
(7) fluorescence excited after optical focus is compensated is emitted into microcobjective 17 out of laboratory sample 18, then
Sequentially pass through rear scan module lens 16, preceding scan module lens 15, rear scanning galvanometer 14, rear light beam collimation lens 13, preceding light
After beam collimation lens 12, preceding scanning galvanometer 11, dichroscope 10, optical filter 19, condenser lens 20 and spatial filter 21
Into photomultiplier 22 carry out light intensity detection, by scanning obtain DMD 5 loading compensation subregion compensate after
Focal beam spot image, as shown in figure 5, the image O in this specific implementation casefThe corresponding light intensity value in position is 202.2.
Traditional optimization adaptive optics focusing technology enters the successive correction of line phase using spatial light modulator.Assuming that
Spatial light modulator is divided into 32 × 32 subregions, each subregion changes the phase of correspondence light beam from 0 to 2 π successively with the interval of π/5
Position, so as to obtain the phase value with best calibration result.Assuming that image loading speed upper limit during spatial light modulator work
For 60Hz, then the time required for completing an optical focus phase detection is：
And the image refresh rate upper limit of DMD used in the present invention is 22727Hz, 32 are equally classified as
× 32 subregions, its complete a sub-beams focus on enhancing subregion judgement time be：
Need successive ignition computing due to optimizing adaptive optics focusing technology, complete time of light beam convergence correction compared with
It is long.And the present invention utilize the quick image refresh rate of DMD, considerably reduce light beam focus on enhancing required for
Processing time, the processing speed of optical focus compensation has been obviously improved it.
As seen from the above-described embodiment, adaptive optics of the present invention focus on interference compensation method and simple system, it is convenient, can
After simple, quick serial light beam judges, the light intensity of target focus point is lifted about 1183%.Compared to utilization space light
Modulator carries out gradually phasing, and the present invention can shorten correction time while focal beam spot central light strength is lifted, be
Non- embedded fibers formula light science of heredity light stimulus provides more inexpensive more easily experimental technique, lifting with deep penetration real time imagery
Conventional efficient.
Claims (10)
1. a kind of adaptive optics focuses on interference compensation method, it is characterised in that comprise the following steps：
1) focal plane of object lens does not place laboratory sample, and light beam focusing is carried out with the DMD (5) without loading subregion,
Ideal focusing hot spot is obtained in the focal plane of object lens, the focusing center position O of ideal focusing hot spot is recordedf；
2) laboratory sample is placed in the focal plane of object lens, light intensity detection is carried out with the DMD (5) without loading subregion,
Record obtains focusing center position OfLight intensity value, be used as Intensity threshold Ith；
3) reflecting surface of DMD (5) is divided into polylith region, using each region successively as target area, to target
The reflected beams in region carry out phase compensation, and traversal regional carries out light intensity spy with the DMD (5) of loading subregion
Survey, obtain the focusing center position O that regional corresponding record is arrivedfA series of light intensity values at place；
4) by each light intensity value and Intensity threshold IthIt is compared, and processing obtains judged result in the following ways：
If light intensity value is less than Intensity threshold Ith, then the corresponding region of the light intensity value is to be not required to phase compensation region；
If light intensity value is more than or equal to Intensity threshold Ith, then the corresponding region of the light intensity value is to need phase compensation region；
5) subregion will be compensated according to judged result and will be loaded on DMD and carries out light intensity detection, the shape in laboratory sample
Final optics focus-compensating hot spot into after interference compensation, is O in focusing center positionfPlace inspires stronger fluorescence.
2. a kind of adaptive optics according to claim 1 focuses on interference compensation method, it is characterised in that：
The step 1) in light beam focus on be specifically：Laser launches light beam, after collimator and extender, in digital micromirror device
Reflect, then focused on by object lens on part (5).
3. a kind of adaptive optics according to claim 1 focuses on interference compensation method, it is characterised in that：The step 3)
Middle signified phase compensation is specifically referred to, and the phase difference that a numerical value is π is added in the former phase of light beam.
4. a kind of adaptive optics according to claim 1 focuses on interference compensation method, it is characterised in that：
The step 2), 3) and 5) in light intensity detection be specifically：Laser launches light beam, after collimator and extender, in number
Reflect, then focused on by object lens on sample on word micro mirror element, sample carries fluorescent material, distortion is produced in sample and is dissipated
Penetrate hot spot and inspire fluorescence, the fluorescence inspired is swept after lens focus with photomultiplier (22) and galvanometer again
Retouch detection and obtain the fluoroscopic image that scattering hot spot is inspired, record focusing center position OfLight intensity value.
5. a kind of adaptive optics according to claim 1 focuses on interference compensation method, it is characterised in that：
The step 3) when being carried out light intensity detection with the DMD (5) of loading subregion and is specifically referred to light intensity detection, it is digital
Itself is received the reflected beams produced after incident beam and adjusts reflection angle by the target area on micro mirror element, makes the reflected beams
To side light path return after phase compensation and do not adjust in the reflected light path before reflection angle, while in addition to target area
Other regions keep the reflected beams reflection angle and the step 2) light intensity detection when the reflected beams reflection angle it is identical.
6. a kind of adaptive optics according to claim 1 focuses on interference compensation method, it is characterised in that：
The step 5) specifically referred to according to judged result adjustment subregion：During by DMD, phase is needed to mend after judgement
Repay region and itself is received to the reflected beams adjustment reflection angle produced after incident beam, the reflected beams is carried out to side light path
Return and do not adjusted in the reflected light path before reflection angle after phase compensation, while phase compensation region is not required to after judging keeps anti-
The reflection angle of irradiating light beam and the step 2) light intensity detection when the reflected beams reflection angle it is identical.
7. a kind of adaptive optics according to claim 1 focuses on interference compensation method, it is characterised in that：
The laboratory sample is but is not limited to vivo biological tissue, tissues in vitro, agar block containing bead etc..
8. focus on interference compensation system for implementing a kind of adaptive optics of claim 1 methods described, it is characterised in that：Bag
Include laser (1), beam collimation and expand module, DMD (5), phase compensation block (6), beam splitter (7), shrink beam mould
Block, scan module, dichroscope (10), microcobjective (17), laboratory sample (18) and light intensity detection module；Beam collimation is expanded
Module arrangement is after laser (1), and laser launches the collimated module parallel that expands of light beam and expands after union space is filtered
DMD (5) is mapped to, DMD (5) side exit end is equipped with phase compensation block (6), DMD
(5) positive side exit end is equipped with beam splitter (7) and shrink beam module, and phase compensation block (6) is located at the side of beam splitter (7), number
The side the reflected beams of word micro mirror element (5) are re-reflected into after phase compensation block (6) in beam splitter (7), digital micromirror device
The positive side the reflected beams of part (5) are directly reflexed in beam splitter (7)；Dichroscope (10) is provided with front of shrink beam module；Light beam is passed through
Focused on after dichroscope (10) reflection through over-scanning module into microcobjective (17), laboratory sample (18) is located at microcobjective
(17) on focal plane；The fluorescence inspired in laboratory sample (18) passes through dichroic after microcobjective (17) and scan module
Mirror (10) is received by light intensity detection module and carries out light intensity detection.
9. a kind of adaptive optics according to claim 8 focuses on interference compensation system, it is characterised in that：Described light beam
Collimator and extender module expands module lens (2), light beam spatial filter (3) and rear beam collimation including preceding beam collimation and expands mould
Block lens (4)；Preceding beam collimation expands module lens (2), light beam spatial filter (3) and rear beam collimation and expands module lens
(4) front of laser (1) exit end is arranged in parallel in successively, and premenstrual beam collimation expands module lens to incident beam successively
(2), light beam spatial filter (3) and rear beam collimation incide DMD after expanding that module lens (4) are parallel and expanding
(5)；
Described shrink beam module includes preceding shrink beam module lens (8) and rear shrink beam module lens (9)；Preceding shrink beam module lens (8)
Be arranged in parallel in the front side of beam splitter (7) successively with rear shrink beam module lens (9), the light beam of DMD (5) reflection according to
Dichroscope is incided after secondary shrink beam parallel through beam splitter (7), preceding shrink beam module lens (8) shrink beam module lens (9) with after
(10) side；
Described scan module include preceding scanning galvanometer (11), preceding light beam collimation lens (12), rear light beam collimation lens (13), after
Scanning galvanometer (14), preceding scan module lens (15) and rear scan module lens (16)；Preceding scanning galvanometer (11), preceding beam collimation
Lens (12), rear light beam collimation lens (13), rear scanning galvanometer (14), preceding scan module lens (15) and rear scan module lens
(16) be sequentially arranged in the front side of dichroscope (10), the light beam of dichroscope (10) reflection successively premenstrual scanning galvanometer (11),
Preceding light beam collimation lens (12), rear light beam collimation lens (13), rear scanning galvanometer (14), preceding scan module lens (15) and after sweep
Retouch and microcobjective (17) is incided after module lens (16)；
Described light intensity detection module includes optical filter (19), condenser lens (20), spatial filter (21) and photoelectricity times
Increase pipe (22)；Optical filter (19), condenser lens (20), spatial filter (21) and photomultiplier (22) are sequentially arranged in
The rear side of dichroscope (10), the fluorescence that laboratory sample (18) is sent successively through microcobjective (17), after scan module lens
(16), preceding scan module lens (15), rear scanning galvanometer (14), rear light beam collimation lens (13), preceding light beam collimation lens (12),
Preceding scanning galvanometer (11), dichroscope (10), optical filter (19), condenser lens (20) and spatial filter (21) enter afterwards
Photomultiplier (22) carries out light intensity detection.
10. a kind of adaptive optics according to claim 8 focuses on interference compensation system, it is characterised in that：Described phase
Position compensating module (6) includes but is not limited to optical delay line, spatial light modulator, phase delay chip.
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| CN109683312A (en) * | 2019-01-22 | 2019-04-26 | 中国工程物理研究院激光聚变研究中心 | A kind of adjusting method of adaptive optics system Image relaying relationship |
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| CN101339129A (en) * | 2007-09-03 | 2009-01-07 | 深圳大学 | Variable visual field scanning microscope and the method based on fixed light path system |
| CN102798622A (en) * | 2012-08-17 | 2012-11-28 | 浙江大学 | Intensity difference based three-dimensional super-resolution microscopic method and device |
| CN103399413A (en) * | 2013-07-30 | 2013-11-20 | 浙江大学 | Double helix light beam-based sample axial drift detection and compensation method and device |
| CN104062750A (en) * | 2014-06-18 | 2014-09-24 | 浙江大学 | Method and device for two-photon fluorescence stimulated emission differential super-resolution microscopy |
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| CN101339129A (en) * | 2007-09-03 | 2009-01-07 | 深圳大学 | Variable visual field scanning microscope and the method based on fixed light path system |
| CN102798622A (en) * | 2012-08-17 | 2012-11-28 | 浙江大学 | Intensity difference based three-dimensional super-resolution microscopic method and device |
| CN103399413A (en) * | 2013-07-30 | 2013-11-20 | 浙江大学 | Double helix light beam-based sample axial drift detection and compensation method and device |
| CN104062750A (en) * | 2014-06-18 | 2014-09-24 | 浙江大学 | Method and device for two-photon fluorescence stimulated emission differential super-resolution microscopy |
Cited By (2)
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| CN109683312A (en) * | 2019-01-22 | 2019-04-26 | 中国工程物理研究院激光聚变研究中心 | A kind of adjusting method of adaptive optics system Image relaying relationship |
| CN109683312B (en) * | 2019-01-22 | 2021-03-12 | 中国工程物理研究院激光聚变研究中心 | Method for adjusting image transfer relationship of adaptive optical system |
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