CN107118276B

CN107118276B - Monoclonal antibody targeting human tumor stem cells and application thereof

Info

Publication number: CN107118276B
Application number: CN201710485302.6A
Authority: CN
Inventors: 沈敏
Original assignee: Suzhou Bojuhua Biology Medicine Technology Co ltd
Current assignee: Suzhou Bojuhua Biology Medicine Technology Co ltd
Priority date: 2017-06-23
Filing date: 2017-06-23
Publication date: 2020-12-04
Anticipated expiration: 2037-06-23
Also published as: CN107118276A

Abstract

The invention relates to the field of biomedicine. In particular, the present invention relates to a mouse monoclonal antibody or an antigen-binding fragment thereof against human tumor stem cells, and hybridoma cells producing the antibody. The invention also relates to the use of said antibodies in the treatment and diagnosis of tumors.

Description

Monoclonal antibody targeting human tumor stem cells and application thereof

Technical Field

Background

Malignant tumors (cancers) have become the first killers threatening the life and health of people all over the world. The number of tumor patients is more than 1400 million every year in the world, and only more than 300 million new tumor patients are added every year in China.

The underlying cause of high mortality from cancer is the spread of cancer cells, metastasis and the susceptibility to relapse and drug resistance in most patients after treatment. The existing clinical treatment means, such as surgery, radiotherapy and chemotherapy, have little curative effect on cancer cell metastasis, recurrence and drug resistance, or only have short-term curative effect, and cannot change the long-term survival condition of patients. Currently, surgical resection works well in about 10-20% of patients in the early stages, but is almost ineffective in patients who have developed diffuse metastases. Radiotherapy can only treat local focus, and is often used as adjuvant therapy before and after operation and radical therapy for a few kinds of cancers. Chemotherapy is available for patients who have developed diffuse metastases, but has significant near term efficacy in only about 20-30% of patients due to the high toxic side effects that tend to develop near or far term resistance. Even with the combination of surgery, radiotherapy and chemotherapy, the long-term efficacy for 5 years of survival has been wandering for years to 20-30%, with about 70-80% of patients dying within 5 years after treatment due to metastasis, relapse and drug resistance. Even in early cancer patients who have no metastasis at the time of treatment, some of them die due to recurrence of metastasis after treatment. The novel targeted medicine for the tumor developed in recent years, which comprises polypeptide, small molecules, protein factors, gene therapy and antibody medicines, can prolong the life of a patient by 3-9 months usually only compared with the existing treatment means when being combined with chemotherapeutic medicines, and does not obviously improve the 5-year survival rate of a long-term patient. In recent two years, emerging tumor immunotherapy approaches, such as PD-1 mab drugs and CAR-T cell therapy, have shown some encouraging indication of long-term efficacy, but the overall efficiency of cancer patients is only about 20-30%, and there are still a large number of cancer patients who cannot be treated with truly effective drugs. Therefore, the key to improve the long-term curative effect and prolong the life of the tumor patients is to develop a novel medicament for inhibiting tumor metastasis, recurrence and drug resistance.

Summary of The Invention

In a first aspect, the present invention provides a monoclonal antibody or an antigen-binding fragment thereof, which is produced by mouse hybridoma cells deposited with the general microbiological center of the China Committee for culture Collection of microorganisms under the accession number CGMCC No.12251 on 16/3/2016.

In a second aspect, the present invention provides a hybridoma cell, which is deposited in the China general microbiological culture Collection center at 2016, 3, 16 days with the collection number of CGMCC No. 12251.

In a third aspect, the present invention provides a pharmaceutical composition comprising a monoclonal antibody of the invention, or an antigen-binding fragment thereof, and a pharmaceutically acceptable carrier.

In some embodiments, the monoclonal antibody or antigen-binding fragment thereof is conjugated to a therapeutic moiety selected from the group consisting of a cytotoxin, a radioisotope, or a biologically active protein.

In a fourth aspect, the present invention provides a method of treating a malignancy, preventing and/or treating metastasis or recurrence of a malignancy in a patient, the method comprising administering to the patient an effective amount of a monoclonal antibody or antigen-binding fragment thereof of the invention or a pharmaceutical composition of the invention.

In some embodiments, the malignancy is selected from breast cancer, colorectal cancer, pancreatic cancer, prostate cancer, liver cancer, lung cancer, and gastric cancer.

In some embodiments, the method further comprises administering to the patient other anti-tumor therapeutic means, such as administration of chemotherapeutic agents, antibodies targeting other tumor-specific antigens, or radiation therapy.

In a fifth aspect, the present invention provides the use of a monoclonal antibody or antigen-binding fragment thereof of the invention or a pharmaceutical composition of the invention in the preparation of a medicament for the treatment of a malignant tumor, the prevention and/or treatment of metastasis or recurrence of a malignant tumor.

In a sixth aspect, the present invention provides a method of detecting the presence of tumor stem cells in a biological sample, comprising:

a) contacting the biological sample with a monoclonal antibody or antigen-binding fragment thereof of the invention;

b) detecting binding of the monoclonal antibody or antigen-binding fragment thereof of the invention to a target antigen in the biological sample, wherein detection of said binding is indicative of the presence of tumor stem cells in the biological sample.

In a seventh aspect, the present invention also provides a method for isolating tumor stem cells, the method comprising:

(a) providing a population of cells suspected of comprising tumor stem cells;

(b) identifying a subpopulation of said cells that binds to a monoclonal antibody of the invention, or an antigen-binding fragment thereof; and

(c) isolating the subpopulation.

In some embodiments of the sixth and seventh aspects, the tumor stem cell is selected from the group consisting of a breast cancer stem cell, a large bowel cancer stem cell, a pancreatic cancer stem cell, a prostate cancer stem cell, a liver cancer stem cell, a lung cancer stem cell, and a gastric cancer stem cell.

In an eighth aspect, the invention also provides a method of detecting the presence of a malignant tumor in a patient, comprising:

a) contacting a biological sample obtained from the patient with a monoclonal antibody or antigen-binding fragment thereof of the invention;

b) detecting binding of the monoclonal antibody or antigen binding fragment thereof of the invention to a target antigen in the biological sample, wherein detection indicates the presence of a malignancy in the patient.

In a ninth aspect, the present invention also provides a method for prognosing recurrence or progression of a malignant tumor in a patient, the method comprising:

(a) isolating a biological sample comprising circulating cells from the patient;

(b) contacting the biological sample comprising circulating cells with a monoclonal antibody or antigen-binding fragment thereof of the invention; and

(c) identifying the presence of circulating cells that bind to the monoclonal antibody or antigen-binding fragment thereof of the invention,

thereby prognosing the recurrence or progression of malignant tumors in said patient.

In some embodiments, the progression of the malignancy comprises metastasis of the malignancy in the patient.

In some embodiments of the eighth and ninth aspects, the biological sample comprises a blood sample, a lymph sample, or a component thereof. In some embodiments, the malignancy is selected from breast cancer, colorectal cancer, pancreatic cancer, prostate cancer, liver cancer, lung cancer, and gastric cancer.

Drawings

FIG. 1. the living cell immunofluorescence technique detects the expression of the monoclonal antibody Hetumomab target antigen on the surface of the living cells of various tumor cells (partial typical positive results).

FIG. 2 shows that the monoclonal antibody Hetumomab target antigen is specifically and highly expressed in human liver cancer, lung cancer and gastric cancer tissues through immunohistochemical detection (partial typical positive results).

FIG. 3 shows that cancer cells identified by monoclonal antibody Hetumomab are significantly enriched in sphere culture cells of various human tumor cell lines (part of typical flow-type fluorescence maps).

FIG. 4 is a CCK8 method for detecting drug resistance of Hetumomab + cells of various human tumor cells (such as liver cancer, lung cancer and gastric cancer) identified by monoclonal antibody Hetumomab (IC 50).

FIG. 5 shows that the monoclonal antibody Hetumomab can significantly inhibit the self-renewal ability (balling) of tumor stem cells of various tumors (such as liver cancer, lung cancer and gastric cancer).

FIG. 6 shows that the monoclonal antibody Hetumomab can obviously inhibit the invasion capacity of tumor stem cells of various tumors (such as liver cancer, lung cancer and gastric cancer).

FIG. 7, the monoclonal antibody Hetumomab can obviously inhibit the invasion capacity of tumor stem cells of various tumors.

FIG. 8 shows the in vivo tumor growth curve of the monoclonal antibody Hetumomab and the combined chemotherapeutic drug for treating human hepatoma transplantable tumor Bel 7402-V13.

FIG. 9 shows the in vivo tumor volume inhibition rate (at drug withdrawal) of the monoclonal antibody Hetumomab and the combined chemotherapeutic drugs for treating human hepatoma transplantable tumor Bel 7402-V13.

FIG. 10 shows the in vivo tumor volume inhibition rate (one month after drug withdrawal) of the monoclonal antibody Hetumomab and the combined chemotherapeutic drugs for treating the human hepatoma transplantable tumor Bel 7402-V13.

FIG. 11 shows the survival curves of mice treated with Hetumomab and combined chemotherapeutic drugs for human hepatoma transplantable tumor Bel 7402-V13.

FIG. 12 is a graph of the in vivo tumor growth of monoclonal antibody Hetumomab on human lung cancer transplantation tumor SPCA-1.

FIG. 13 is the in vivo tumor growth curve of the monoclonal antibody Hetumomab and the combination chemotherapeutic drug for treating human gastric cancer transplantation tumor SNU-5.

Detailed Description

A, define

In the present invention, unless otherwise specified, scientific and technical terms used herein have the meanings that are commonly understood by those skilled in the art. Also, protein and nucleic acid chemistry, molecular biology, cell and tissue culture, microbiology, immunology related terms, and laboratory procedures used herein are all terms and conventional procedures used extensively in the relevant art. Meanwhile, in order to better understand the present invention, the definitions and explanations of related terms are provided below.

As used herein, "antibody" refers to immunoglobulins and immunoglobulin fragments, whether naturally occurring or partially or wholly synthetically (e.g., recombinantly) produced, including any fragments thereof that comprise at least a portion of the variable region of an immunoglobulin molecule that retain the binding specificity capability of a full-length immunoglobulin. Thus, an antibody includes any protein having a binding domain that is homologous or substantially homologous to an immunoglobulin antigen binding domain (antibody binding site). Antibodies include antibody fragments, such as anti-tumor stem cell antibody fragments. As used herein, the term antibody thus includes synthetic antibodies, recombinantly produced antibodies, multispecific antibodies (e.g., bispecific antibodies), human antibodies, non-human antibodies, humanized antibodies, chimeric antibodies, intrabodies, and antibody fragments, such as, but not limited to, Fab fragments, Fab 'fragments, F (ab')₂Fragments, Fv fragments, disulfide-linked Fv (dsfv), Fd fragments, Fd' fragments, single chain Fv (scFv), single chain Fab (scFab), diabodies, anti-idiotypic (anti-Id) antibodies, or antigen-binding fragments of any of the foregoing. Antibodies provided herein include members of any immunoglobulin class (e.g., IgG, IgM, IgD, IgE, IgA, and IgY), any class (e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2), or subclass (e.g., IgG2a and IgG2b)。

As used herein, an "antibody fragment" or "antigen-binding fragment" of an antibody refers to any portion of a full-length antibody that is less than full-length, but that comprises at least a portion of the variable region of the antibody that binds antigen (e.g., one or more CDRs and/or one or more antibody binding sites), and thus retains the binding specificity as well as at least a portion of the specific binding capacity of the full-length antibody. Thus, an antigen-binding fragment refers to an antibody fragment that comprises an antigen-binding portion that binds to the same antigen as the antibody from which the antibody fragment is derived. Antibody fragments include antibody derivatives produced by enzymatic treatment of full-length antibodies, as well as synthetically produced derivatives, e.g., recombinantly produced derivatives. Antibodies include antibody fragments. Examples of antibody fragments include, but are not limited to, Fab ', F (ab')₂Single chain Fv (scFv), Fv, dsFv, diabodies, Fd and Fd' fragments, and other fragments, including modified fragments (see, e.g., Methods in Molecular Biology, Vol 207: Recombinant Antibodies for Cancer Therapy Methods and Protocols (2003); Chapter 1; p 3-25, Kipriyanov). The fragments may comprise multiple chains linked together, for example by disulphide bonds and/or by peptide linkers. Antibody fragments generally comprise at least or about 50 amino acids, and typically at least or about 200 amino acids. Antigen-binding fragments include any antibody fragment that, when inserted into an antibody framework (e.g., by replacement of the corresponding region), achieves immunospecific binding (i.e., exhibits at least or at least about 10)⁷-10⁸M^-1Ka) of (2).

As used herein, "monoclonal antibody" refers to a population of identical antibodies, meaning that each individual antibody molecule in the monoclonal antibody population is identical to other antibody molecules. This property is in contrast to the property of a polyclonal population of antibodies that comprises antibodies having a plurality of different sequences. Monoclonal antibodies can be prepared by a number of well-known methods (Smith et al (2004) J. Clin. Pathol.57, 912-917; and Nelson et al, J Clin Pathol (2000),53, 111-. For example, monoclonal antibodies can be prepared by immortalizing B cells, e.g., by fusion with myeloma cells to produce hybridoma cell lines or by infecting B cells with a virus such as EBV. Recombinant techniques can also be used to produce antibodies in vitro from a clonal population of host cells by transforming the host cells with plasmids carrying artificial sequences of nucleotides encoding the antibodies.

As used herein, the term "hybridoma" or "hybridoma cell" refers to a cell or cell line (typically a myeloma or lymphoma cell) resulting from the fusion of an antibody-producing lymphocyte and a non-antibody-producing cancer cell. As known to those of ordinary skill in the art, hybridomas proliferate and continuously supply production of a particular monoclonal antibody. Methods for producing hybridomas are known in the art (see, e.g., Harlow & Lane, 1988). When referring to the term "hybridoma" or "hybridoma cell", it also includes subclones and progeny cells of the hybridoma.

As used herein, the term "epitope" refers to any antigenic determinant on an antigen to which the paratope of an antibody binds. Epitopic determinants generally comprise a chemically active surface type of molecule, such as amino acid or sugar side chains, and generally have specific three-dimensional structural characteristics as well as specific charge characteristics.

As used herein, "specifically binds" or "immunospecifically binds" with respect to an antibody or antigen-binding fragment thereof is used interchangeably herein and refers to the ability of the antibody or antigen-binding fragment to form one or more non-covalent bonds with the alloantigen through non-covalent interactions between the antibody and the antibody-binding site of the antigen. The antigen may be an isolated antigen or present in a tumor cell. Typically, the antibody that immunospecifically binds (or specifically binds) to the antigen is at about or 1 × 10⁷M^-1Or 1x 10⁸M^-1Or greater affinity constant Ka (or 1x 10)^-7M or 1X 10^-8Dissociation constant (K) of M or less_d) Bind the antigen. Affinity constants can be determined by standard kinetic methods of antibody reaction, e.g., immunoassay, Surface Plasmon Resonance (SPR) (Rich and Myszka (2000) curr. Opin. Biotechnol 11: 54; Englebienne (1998) analysis.123: 1599), Isothermal Titration Calorimetry (ITC), or other kinetic interaction assays known in the art (see, e.g., U.S.A.)For example, Paul, ed., Fundamental Immunology,2nd ed., Raven Press, New York, pages 332-; see also U.S. patent No. 7,229,619 describing exemplary SPR and ITC methods for calculating binding affinity of an antibody). Instruments and methods for detecting and monitoring the rate of binding in real time are known and commercially available (see, BiaCore 2000, Biacore AB, Upsala, Sweden and GE Healthcare Life Sciences; Malmqvist (2000) biochem. Soc. Trans.27: 335).

As used herein, the term "competes" with respect to an antibody means that the first antibody or antigen-binding fragment thereof binds to an epitope in a manner sufficiently similar to the second antibody or antigen-binding fragment thereof such that the binding outcome of the first antibody to its cognate epitope is detectably reduced in the presence of the second antibody as compared to the absence of the second antibody. Alternatively, this may, but need not, be the case where the binding of the second antibody to its epitope is also detectably reduced in the presence of the first antibody. That is, the first antibody may inhibit the binding of the second antibody to its epitope without the second antibody inhibiting the binding of the first antibody to its respective epitope. However, in the case where each antibody detectably inhibits the binding of another antibody to its cognate epitope or ligand, whether to the same, greater or lesser extent, the antibodies are said to "cross-compete" with each other for binding to their respective epitopes. Both competing and cross-competing antibodies are encompassed by the present invention. Regardless of the mechanism by which such competition or cross-competition occurs (e.g., steric hindrance, conformational change, or binding to a common epitope or fragment thereof), one of skill in the art, based on the teachings provided herein, will recognize that such competing and/or cross-competing antibodies are encompassed by the present invention and can be used in the methods disclosed herein.

As used herein, "polypeptide" refers to two or more amino acids that are covalently linked. The terms "polypeptide" and "protein" are used interchangeably herein.

An "isolated protein," "isolated polypeptide," or "isolated antibody" means that the protein, polypeptide, or antibody is (1) not associated with components with which it is naturally associated in its native state, (2) free of other proteins from the same species, (3) expressed by cells from a different species, or (4) does not occur in nature. Thus, a chemically synthesized polypeptide or a polypeptide synthesized in a cell system different from the cell from which the polypeptide is naturally derived will be "isolated" from its naturally associated components. The protein may also be isolated so as to be substantially free of naturally associated components, i.e., using protein purification techniques well known in the art.

As used herein, "treating" an individual having a disease or condition means that the individual's symptoms are partially or fully alleviated, or remain unchanged after treatment. Thus, treatment includes prophylaxis, treatment and/or cure. Prevention refers to prevention of the underlying disease and/or prevention of worsening of symptoms or disease progression. Treatment also includes any antibody or antigen-binding fragment thereof provided as well as any pharmaceutical use of the compositions provided herein.

As used herein, "therapeutic effect" means an effect resulting from treatment of an individual that alters, typically ameliorates or improves a symptom of a disease or disease condition, or cures the disease or disease condition.

As used herein, "therapeutically effective amount" or "therapeutically effective dose" refers to an amount of a substance, compound, material, or composition comprising a compound that is at least sufficient to produce a therapeutic effect upon administration to a subject. Thus, it is the amount necessary to prevent, cure, ameliorate, block, or partially block the symptoms of the disease or disorder.

As used herein, a "prophylactically effective amount" or a "prophylactically effective dose" refers to an amount of a substance, compound, material, or composition comprising a compound that will have the intended prophylactic effect when administered to a subject, e.g., to prevent or delay the onset or recurrence of a disease or symptom, to reduce the likelihood of onset or recurrence of a disease or symptom. A complete prophylactically effective dose need not occur by administration of one dose, and may occur only after administration of a series of doses. Thus, a prophylactically effective amount may be administered in one or more administrations.

As used herein, the term "patient" refers to a mammal, such as a human.

"tumor stem cells" refers to a small fraction of cancer cells with a dry characteristic present in tumor tissues, which have self-renewal capacity, strong invasive capacity, resistance to chemotherapeutic drugs, and strong tumorigenic capacity compared to normal cancer cells.

Monoclonal antibody for tumor stem cells

In the invention, a human tumor pluripotent stem cell line is used as immunogen to immunize a mouse, and a monoclonal antibody Hetumomab is obtained by a classical hybridoma fusion technology. The mouse hybridoma cell strain Hetumomab producing the monoclonal antibody Hetumomab is preserved in China general microbiological culture Collection center (CGMCC No. 12251) at 2016, 3, 16 and 3. (example 1)

The inventor finds that the target antigen of the monoclonal antibody Hetumomab is expressed on the surfaces of living cells of various human tumor cells, and is specifically and highly expressed in various tumor tissues (the positive rate is 79-94%). The Hetumomab monoclonal antibody disclosed by the invention can be enriched in the sphere culture cells of various tumor cells and can identify tumor cells of tumor stem cell markers such as ESA (embryonic stem cell assay), CD90 and the like, and the Hetumomab monoclonal antibody is a monoclonal antibody specific to the tumor stem cells (example 2).

Further studies based on the hetomomab target antigen positive tumor cells showed that the hetomomab target antigen positive tumor cells had stronger self-renewal, invasion, drug resistance and in vivo tumorigenic capacity relative to the parental tumor cells and the hetomomab target antigen negative tumor cells, further demonstrating that the hetomomab specifically targets tumor stem cells (example 3).

The tumor stem cells specifically targeted by the Hetumomab monoclonal antibody of the invention include, but are not limited to, breast cancer stem cells, large intestine cancer stem cells, pancreas cancer stem cells, prostate cancer stem cells, liver cancer stem cells, lung cancer stem cells and stomach cancer stem cells.

Therefore, the invention provides a monoclonal antibody or an antigen binding fragment thereof, wherein the monoclonal antibody is produced by mouse hybridoma cells which are preserved in China general microbiological culture Collection center (CGMCC No. 12251) at 3, 16 and 2016.

The invention also encompasses isolated monoclonal antibodies or antigen-binding fragments thereof that compete with Hetumomab for binding to tumor stem cells.

The invention also encompasses isolated monoclonal antibodies or antigen binding fragments thereof that bind to the same epitope on tumor stem cells as Hetumomab.

Third, disease treatment

Tumor stem cells are a small fraction of cancer cells present in tumor tissue with a sternness characteristic, with the following biological characteristics: can self-renew, duplicate, differentiate nondirectionally, and has high tumorigenicity, high invasion, diffusion and transfer capacity, and no sensitivity to radiotherapy and chemotherapy. Due to the existence of the tumor stem cells, the tumor can continuously and rapidly grow, spread, metastasize and recur. More serious, the tumor stem cells are resistant to almost all traditional chemotherapy drugs, radiotherapy and targeted drugs (including antibody targeted drugs) on the market in recent years. The tumor stem cells are all in the non-growth and non-proliferation state in the G0 stage of the cell cycle. Chemoradiotherapy only works on cancer cells that grow and proliferate at a high speed, but cannot kill tumor stem cells in the G0 stage. When a large amount of cancer cells growing rapidly are killed by the chemoradiotherapy, the tumor stem cells resisting the chemoradiotherapy are screened and enriched, so that the proportion is greatly improved. Because the tumor stem cells have extremely strong self-replication capacity and diffusion and transfer capacity, the tumor stem cells can be rapidly differentiated, proliferated, grown and spread and transferred to all organs of the whole body to form new transfer focuses, and the cancer cells of the transfer focuses resist radiotherapy and chemotherapy, and the existence of the tumor stem cells is proved in various malignant tumors such as breast cancer, colorectal cancer, pancreatic cancer, prostatic cancer, liver cancer, lung cancer, gastric cancer and the like. The more malignant cancer, the more tumor stem cells, and the higher the proportion of tumor stem cells, the more metastatic and recurrent cancer patients, the shorter the survival time.

The inventors found that the Hetumomab monoclonal antibody specifically recognizing tumor stem cells of the present invention can significantly inhibit self-renewal, invasion and drug resistance of various tumor stem cells in vitro (example 4). Further experiments showed that the Hetumomab mab was able to inhibit the growth, metastasis and drug resistance of various tumor transplants in animal models (example 5). Therefore, the Hetumomab mabs of the invention can be used to treat malignancies, prevent or/and treat malignant metastasis or recurrence by targeting tumor stem cells.

Thus, the present invention provides a method of treating a malignancy, preventing or/and treating metastasis or recurrence of a malignancy in a patient, the method comprising administering to the patient an effective amount of an antibody or antigen-binding fragment thereof against tumor stem cells of the present invention. Malignant tumors that can be treated by the methods of the present invention include, but are not limited to, breast cancer, colorectal cancer, pancreatic cancer, prostate cancer, liver cancer, lung cancer, and gastric cancer.

Medicine composition

The invention also provides a pharmaceutical composition comprising the antibody or antigen-binding fragment thereof against tumor stem cells of the invention and a pharmaceutically acceptable carrier. The pharmaceutical composition is used for treating malignant tumors, preventing or/and treating metastasis or recurrence of malignant tumors in a patient.

As used herein, "pharmaceutically acceptable carrier" includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, that are physiologically compatible. Preferably, the carrier is suitable for intravenous, intramuscular, subcutaneous, parenteral, spinal or epidermal administration (e.g., by injection or infusion). Depending on the route of administration, the active compound, i.e., antibody molecule, immunoconjugate, may be encapsulated in a material to protect the compound from acids and other natural conditions that may inactivate the compound.

The pharmaceutical compositions of the present invention may also contain a pharmaceutically acceptable antioxidant. Examples of pharmaceutically acceptable antioxidants include: (1) water-soluble antioxidants such as ascorbic acid, cysteine hydrochloride, sodium bisulfate, sodium metabisulfite, sodium sulfite, and the like; (2) oil-soluble antioxidants such as ascorbyl palmitate, Butylated Hydroxyanisole (BHA), Butylated Hydroxytoluene (BHT), lecithin, propyl gallate, alpha-tocopherol, and the like; and (3) metal chelating agents such as citric acid, ethylenediaminetetraacetic acid (EDTA), sorbitol, tartaric acid, phosphoric acid, and the like.

These compositions may also contain adjuvants such as preserving, wetting, emulsifying, and dispersing agents.

Prevention of the presence of microorganisms can be ensured by sterilization procedures or by the inclusion of various antibacterial and antifungal agents, such as parabens, chlorobutanol, phenol sorbic acid, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol, sorbitol, or sodium oxide in the composition. Prolonged absorption of the injectable drug can be achieved by incorporating into the composition a delayed absorption agent, such as monostearate salts and gelatin.

Pharmaceutically acceptable carriers include sterile aqueous solutions or dispersions and powders for the extemporaneous preparation of sterile injectable solutions or dispersions. The use of such media and agents for pharmaceutically active substances is well known in the art. Conventional media or agents, except insofar as any is incompatible with the active compound, may be present in the pharmaceutical compositions of the invention. Supplementary active compounds may also be incorporated into the composition.

Therapeutic compositions generally must be sterile and stable under the conditions of manufacture and storage. The compositions may be formulated as solutions, microemulsions, liposomes or other ordered structures suitable for high drug concentrations. The carrier can be a solvent or dispersion containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof. Proper fluidity can be maintained, for example, by the use of a coating, such as lecithin, by the maintenance of the required particle size in the case of dispersions and by the use of surfactants.

Sterile injectable solutions can be prepared by incorporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by sterile microfiltration. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle which contains a basic dispersion medium and the required other ingredients from those enumerated above. For sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze-drying (lyophilization) that yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.

The amount of active ingredient that can be combined with the carrier materials to produce a single dosage form will vary depending upon the subject being treated and the particular mode of administration. The amount of active ingredient that can be combined with the carrier materials to produce a single dosage form is generally that amount of the composition which produces a therapeutic effect. Typically, this amount ranges from about 0.01% to about 99% of the active ingredient, preferably from about 0.1% to about 70%, most preferably from about 1% to about 30%, by 100%, in combination with a pharmaceutically acceptable carrier.

Dosage regimens may be adjusted to provide the best desired response (e.g., therapeutic response). For example, a single bolus may be administered, several divided doses may be administered over time, or the dose may be scaled down or up as required by the exigencies of the therapeutic condition. It is particularly advantageous to formulate parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suitable as unitary dosages for the subject to be treated; each unit containing a predetermined amount of active compound calculated to produce the desired therapeutic effect in combination with the required pharmaceutical carrier. The specifics of the dosage unit forms of the invention are defined and directly dependent upon (a) the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and (b) the limitations inherent in the art of formulating such active compounds for use in the treatment of sensitivity in an individual.

For administration of the antibody molecule, the dosage range is about 0.0001 to 100mg/kg, more usually 0.01 to 20mg/kg of the recipient's body weight. For example, the dose may be 0.3mg/kg body weight, 1mg/kg body weight, 3mg/kg body weight, 5mg/kg body weight, 10mg/kg body weight or 20mg/kg body weight, or in the range of 1-20mg/kg body weight. Exemplary treatment regimens require weekly dosing, biweekly dosing, every three weeks, every four weeks, monthly dosing, every 3 months, every 3-6 months, or slightly shorter initial dosing intervals (e.g., weekly to every three weeks) followed by longer post dosing intervals (e.g., monthly to every 3-6 months).

Alternatively, antibody molecules directed against tumor stem cells may also be administered as a sustained release formulation, in which case less frequent administration is required. The dose and frequency will vary depending on the half-life of the antibody molecule in the patient. Typically, human antibodies exhibit the longest half-life, followed by humanized, chimeric, and non-human antibodies. The dosage and frequency of administration will vary depending on whether the treatment is prophylactic or therapeutic. In prophylactic applications, relatively low doses are administered at less frequent intervals over an extended period of time. Some patients continue to receive treatment for the remainder of their lives. In therapeutic applications, it is sometimes desirable to administer higher doses at shorter intervals until progression of the disease is reduced or halted, preferably until the patient exhibits partial or complete improvement in disease symptoms. Thereafter, the administration to the patient may be carried out in a prophylactic regime.

Actual dosage levels of the active ingredients in the pharmaceutical compositions of this invention may be varied so as to obtain amounts of the active ingredients effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without toxicity to the patient. The selected dosage level will depend upon a variety of pharmacokinetic factors including the activity of the particular composition of the invention employed, the route of administration, the time of administration, the rate of excretion of the particular compound employed, the duration of the treatment, other drugs, compounds and/or materials used in conjunction with the particular composition employed, the age, sex, weight, condition, general health and medical history of the patient being treated, and like factors well known in the medical arts.

An "effective amount" of an antibody or antigen-binding fragment thereof of the invention preferably results in a reduction in the severity of disease symptoms, an increase in the frequency and duration of the asymptomatic phase of the disease, or prevention of injury or disability due to disease affliction. For example, for the treatment of a tumor, an "effective amount" of an antibody or antigen-binding fragment thereof of the invention preferably inhibits cell growth or tumor growth by at least about 10%, preferably by at least about 20%, more preferably by at least about 30%, more preferably by at least about 40%, more preferably by at least about 50%, more preferably by at least about 60%, more preferably by at least about 70%, more preferably by at least about 80%, relative to the untreated subject. The ability to inhibit tumor growth can be evaluated in animal model systems that predict efficacy against human tumors. Alternatively, it can be assessed by examining the ability to inhibit cell growth, which can be measured in vitro by assays well known to those skilled in the art. An effective amount of an antibody or antigen-binding fragment thereof of the invention is capable of reducing tumor size, or otherwise alleviating a symptom in a subject such as preventing and/or treating metastasis or recurrence. Such amounts can be determined by one skilled in the art based on factors such as the size of the subject, the severity of the subject's symptoms, and the particular composition or route of administration selected.

The antibodies or antigen-binding fragments thereof of the present invention or the pharmaceutical compositions of the present invention may be administered by one or more routes of administration using one or more methods well known in the art. It will be appreciated by those skilled in the art that the route and/or manner of administration will vary depending on the desired result. Preferred routes of administration of the antibodies of the invention include intravenous, intramuscular, intradermal, intraperitoneal, subcutaneous, spinal or other parenteral routes of administration, such as injection or infusion. The phrase "parenteral administration" as used herein refers to modes of administration other than enteral and topical administration, typically injections, including, but not limited to, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal, epidural, and intrasternal injections and infusions.

Alternatively, the antibody or antigen-binding fragment thereof against tumor stem cells of the present invention or the pharmaceutical composition of the present invention may also be administered by a non-parenteral route, such as topical, epidermal or mucosal route, e.g., intranasal, oral, vaginal, rectal, sublingual or topical.

The active compounds can be formulated with carriers that protect the compound from rapid release, such as controlled release formulations, including implants, transdermal patches, and microencapsulated delivery systems. Biodegradable, biocompatible polymers may be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Many methods of preparing such formulations are patented or are generally known to those skilled in the art. See, for example, Sustaineedand controlled Release Drug Delivery Systems, J.R. Robinson, ed., Marcel Dekker, Inc., New York, 1978.

The therapeutic compositions can be administered using medical devices well known in the art. For example, in a preferred embodiment, the therapeutic compositions of the present invention can be administered using a needleless hypodermic injection device, such as those described in U.S. Pat. Nos. 5,399,163; 5,383,851, respectively; 5,312,335, respectively; 5,064,413, respectively; 4,941,880, respectively; 4,790,824, respectively; or 4,596,556. Examples of known implants and modules that may be used in the present invention include: U.S. patent No.4,487,603, which discloses an implantable micro-infusion pump for dispensing a drug at a controlled rate; U.S. patent No.4,486,194, which discloses a therapeutic device for transdermal drug delivery; U.S. Pat. No.4,447,233, which discloses a medical infusion pump for delivering a drug at a precise infusion rate; U.S. patent No.4,447,224, which discloses a variable flow implantable infusion device for continuous delivery of a drug; U.S. Pat. No.4,439,196, which discloses an osmotic drug delivery system having multiple lumen compartments: and U.S. patent No.4,475,196, which discloses an osmotic drug delivery system. These patents are incorporated herein by reference. Many other such implants, delivery systems, and modules are known to those skilled in the art.

In certain embodiments, the antibodies of the invention against tumor stem cells can be formulated to ensure proper distribution in vivo. For example, the blood-brain barrier (BBB) prevents many highly hydrophilic compounds. To ensure that the therapeutic compounds of the invention are able to cross the BBB (if desired), they may be formulated, for example, in liposomes. As for methods of preparing liposomes, see, for example, U.S. Pat. nos. 4,522,811; 5,374,548, and 5,399,331. Liposomes contain one or more targeting moieties that can be selectively transported into a particular cell or organ, thereby enhancing targeted drug delivery (see, e.g., v.v. ranade (1989) j.clin.pharmacol.29: 685). Examples of targeting moieties include folate or biotin (see, e.g., U.S. Pat. No.5,416,016 to Low et al); mannoside (Umezawa et al (1988) biochem. Biophys. Res. Commun.153: 1038); antibodies (P.G.Blueman et al (1995) FEBS Lett.357: 140; M.Owais et al (1995) antibodies.Agents Chemother.39: 180); the surfactant protein A receptor (Briscoe et al (1995) am. J. physiol.1233: 134); p120(Schreier et al (1994) J.biol.chem.269: 9090); see also k.keinanen; M.L.Laukkanen (1994) FEBS Lett.346: 123; j.j.killion; fidler (1994) Immunomethods 4: 273.

the antibodies or antigen-binding fragments thereof directed against tumor stem cells of the present invention in the pharmaceutical composition may also be conjugated with a therapeutic moiety such as a cytotoxin, a radioisotope, or a biologically active protein.

Cytotoxins include any agent that is harmful to cells (e.g., kills cells). Examples include: paclitaxel, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, epipodophyllotoxin glucopyranoside, epipodophyllotoxin thiophenoside, vincristine, vinblastine, colchicine, doxorubicin, daunorubicin, dihydroxy anthrax dione, mitoxantrone, mithramycin, actinomycin D, 1-dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, and puromycin and analogs or homologs thereof.

Therapeutic agents useful for conjugation also include, for example: antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6-thioguanine, cytarabine, 5-fluorouracil, dacarbazine), alkylating agents (e.g., mechlorethamine, chlorambucil, melphalan, carmustine (BSNU) and lomustine (CCNU), cyclophosphamide, busulfan, dibromomannitol, streptozocin, mitomycin C and cis-dichlorodiammineplatinum (II) (DDP) cisplatin), anthranilones (e.g., daunorubicin (formerly daunorubicin) and doxorubicin), antibiotics (e.g., actinomycin D (formerly actinomycin), bleomycin, mithramycin and Amphenomycin (AMC)), and antimitotics (e.g., vincristine and vinblastine).

Other preferred examples of therapeutic cytotoxins that can be conjugated to the antibodies of the present invention directed to tumor stem cells include duocarmycin, calicheamicin, maytansine, auristatin, and derivatives thereof.

Cytotoxins may be conjugated to the antibodies of the present invention against tumor stem cells using linker technology used in the art. Examples of types of linkers that have been used to conjugate cytotoxins to antibodies directed against tumor stem cells include, but are not limited to, hydrazones, thioethers, esters, disulfides, and peptide-containing linkers. Alternatively, for example, a linker may be selected that is susceptible to cleavage by low pH or by a protease, such as a protease preferentially expressed in tumor tissue, such as cathepsin (e.g., cathepsin B, C, D), within the lysosome compartment.

For further discussion of the type of cytotoxin, the linker used to conjugate the therapeutic agent to the antibody, and the methods, see Saito, g, et al (2003) adv. drug deliv. rev.55: 199-; trail, p.a. et al (2003) cancer.immunol.immunoher.52: 328-337; payne, G. (2003) Cancer Cell 3: 207-212; allen, t.m. (2002) nat. rev. cancer 2: 750- > 763; patan, i, and Kreitman, R.J, (2002) curr. opin. investig. drugs 3: 1089-; senter, P.D. and Springer, C.J. (2001) adv.drug Deliv.Rev.53: 247-264.

The antibodies of the present invention directed to tumor stem cells may also be conjugated with a radioisotope to produce a cytotoxic radiopharmaceutical, also referred to as a radioactive antibody conjugate. Examples of radioisotopes that can be conjugated to antibodies for diagnostic or therapeutic use include, but are not limited to, iodine 131, indium 111, yttrium 90, and lutetium 177. Methods for preparing radioactive antibody conjugates have been established in the art.

The antibodies of the invention directed to tumor stem cells may also be conjugated to proteins having a desired biological activity, and may be used to modify specific biological responses. Such biologically active proteins include, for example: toxins or active fragments thereof having enzymatic activity, such as abrin, ricin a, pseudomonas exotoxin, or diphtheria toxin; proteins, such as tumor necrosis factor or interferon-gamma; or biological response modifiers such as lymphokines, interleukin-1 ("IL-1"), interleukin-2 ("IL-2"), interleukin-6 ("IL-6"), interleukin-10 ("IL-10"), granulocyte macrophage colony stimulating factor ("GM-CSF"), granulocyte colony stimulating factor ("G-CSF"), or other immune factors such as IFN and the like.

Techniques For conjugating such therapeutic moieties to antibody molecules are well known, see, e.g., Arnon et al, "Monoclonal Antibodies For Immunotargeting Of Drugs In Cancer Therapy", Monoclonal Antibodies And Cancer Therapy, Reisfeld et al (ed.), pp.243-56(Alan R.Liss, Inc.1985); hellstrom et al, "Antibodies For Drug Delivery", Controlled Drug Delivery (2nd Ed.), Robinson et al (Ed.), pp.623-53(Marcel Dekker, Inc.1987); thorpe, "Antibody Carriers Of Cytotoxin Agents In Cancer Therapy: a Review ", Monoclonal Antibodies' 84: biological And Clinical Applications, Pinchera et al (ed.), pp.475-506 (1985); "Analysis, Results, And" Analysis "Of The Therapeutic Use Of radioactive Antibody In Cancer Therapy", Monoclonal Antibodies For Cancer Detection And Therapy, Baldwin et al (ed.), pp.303-16(Academic Press 1985), And "Thorpe et al," The prediction And cytological proteins Of Antibody-Toxin Conjugates, "Immunol.Rev., 62: 119-58(1982).

Combination therapy

The antibodies or pharmaceutical compositions of the invention directed to tumor stem cells may be administered in combination with chemotherapeutic agents or antibodies targeting other tumor antigens. The present application, example 5, demonstrates the synergistic effect of the administration of the Hetumomab monoclonal antibodies of the invention in combination with chemotherapeutic agents in tumor therapy. Without being bound by any theory, it is believed that the antibodies against tumor stem cells of the present invention are capable of inhibiting tumor resistance function, thereby enabling a synergistic effect when administered in combination with chemotherapeutic agents or antibodies targeting other tumor antigens.

Chemotherapeutic agents or antibodies targeting other tumor antigens that can be used in combination with the antibodies of the invention or the pharmaceutical compositions of the invention are not particularly limited. Examples of such chemotherapeutic agents and antibodies targeting other tumor antigens include, but are not limited to: ifosfamide, cyclophosphamide, dacarbazine, temozolomide, nimustine, busulfan, melphalan, enocitabine, capecitabine, carmofur, cladribine, gemcitabine, cytarabine, tegafur-uracil, TS-1, doxifluridine, nelarabine, hydroxyurea, fluorouracil, fludarabine, pemetrexed, pentostatin, mercaptopurine, methotrexate, irinotecan, etoposide, eribulin, sobuzolfacto, docetaxel, paclitaxel, vinorelbine, vincristine, vindesine, vinblastine, actinomycin D, aclarubicin, amrubicin, idarubicin, epirubicin, netfatin, daunorubicin, doxorubicin, pirarubicin, bleomycin, pellomycin, mitomycin C, mitoxantrone, oxaliplatin, carboplatin, cisplatin, nedaplatin, Anastrozole, exemestane, ethinylestradiol, chlordexrazone, goserelin, tamoxifen, dexamethasone, bicalutamide, toremifene, flutamide, prednisolone, fosfestrol, mitotane, methyltestosterone, leuprolide, letrozole, methamphetamine, temitumomab, imatinib, everolimus, erlotinib, gefitinib, sunitinib, cetuximab, sorafenib, dasatinib, tamibarotene, trastuzumab, retinoic acid, pamitumomab, bevacizumab, bortezomib, and lapatinib. In a specific embodiment, the chemotherapeutic agent is a platinum-containing chemotherapeutic agent, such as cisplatin.

The antibody of the invention and the chemotherapeutic agent or antibody targeting the other tumor antigen may all be administered at one time or separately. When administered separately (in the case of mutually different administration regimens), they may be administered continuously without interruption or at predetermined intervals.

The combined dose of the antibody of the invention and the chemotherapeutic agent or antibody targeting another tumor antigen in the pharmaceutical composition of the invention is not particularly limited. As described above, the dose of the antibody of the present invention can be determined by referring to the dose when the antibody is used alone. The chemotherapeutic agent and the antibody targeting the other tumor antigen may be used or may be reduced (in view of the combined effect with the antibody of the present invention) according to the respective drug indicated dose.

The antibody of the invention or the pharmaceutical composition of the invention may also be combined with radiotherapy, for example comprising the administration of ionizing radiation to a patient before, during and/or after the administration of the antibody or pharmaceutical composition of the invention.

Sixthly, detecting and purifying tumor stem cells

As described herein, the Hetumomab monoclonal antibodies of the invention specifically recognize tumor stem cells. Accordingly, the present invention also provides a method of detecting the presence of tumor stem cells in a biological sample comprising:

b) detecting binding of the monoclonal antibody or antigen binding fragment thereof of the invention to a target antigen in the biological sample, wherein detection indicates the presence of tumor stem cells in the biological sample.

In some embodiments, the tumor stem cell is selected from the group consisting of a breast cancer stem cell, a large bowel cancer stem cell, a pancreatic cancer stem cell, a prostate cancer stem cell, a liver cancer stem cell, a lung cancer stem cell, and a gastric cancer stem cell.

In some embodiments of the above-described detection methods of the invention, the monoclonal antibody or antigen-binding fragment thereof of the invention is further conjugated with a fluorescent dye, chemical, polypeptide, enzyme, isotope, tag, or the like that is detectable or can be detected by other reagents.

Methods for detecting antibody-antigen binding are known in the art, such as ELISA and the like.

The present invention also provides a method for isolating tumor stem cells, the method comprising:

(a) providing a population of cells suspected of comprising tumor stem cells;

(c) isolating the subpopulation.

For example, liver cancer stem cells can be isolated by flow cytometry.

Seventh, diagnosis and prognosis

As described herein, the target antigen of the monoclonal antibody Hetumomab of the present invention is expressed on the surface of living cells of various human tumor cells, and is specifically highly expressed in various tumor tissues (positive rate 79% -94%).

Accordingly, the present invention also provides a method of detecting the presence of a malignant tumor in a patient, comprising:

The present invention also provides a method for prognosing recurrence or progression of a malignant tumor in a patient, the method comprising:

The presence of circulating cells identified that bind to the monoclonal antibody or antigen-binding fragment thereof of the invention is indicative of a high risk of recurrence or progression of malignant tumors in said patient.

In some embodiments, the biological sample comprises a blood sample, a lymph sample, or a component thereof.

In some embodiments of the above-described methods of the invention, the monoclonal antibody or antigen-binding fragment thereof of the invention is further conjugated to a fluorescent dye, chemical, polypeptide, enzyme, isotope, tag, or the like, which is detectable or detectable by other reagents.

Eighthly, kit

Also within the scope of the invention are kits for use in the methods of the invention, the kits comprising a monoclonal antibody of the invention, or an antigen-binding fragment thereof, and instructions for use. The kit may further comprise at least one additional detection reagent for detecting the presence of a monoclonal antibody of the invention. The kit generally includes a label indicating the intended use and/or method of use of the kit contents. The term label includes any written or recorded material provided on or with the kit or otherwise provided with the kit.

Examples

A further understanding of the present invention may be obtained by reference to certain specific examples which are set forth herein and are intended to be illustrative of the invention only and are not intended to limit the scope of the invention in any way. Obviously, many modifications and variations of the present invention are possible without departing from the spirit thereof, and these modifications and variations are therefore also within the scope of the invention as claimed.

Example 1 preparation of mouse monoclonal antibody Hetumomab

Preparation of anti-human tumor pluripotent stem cell mouse monoclonal antibody library

This example used a human tumor pluripotent stem cell line T3A-A3 as the immunogen (Liu H, et al. cell Death and disease.2013,4: e 857). The human tumor stem cell line is obtained by separating liver cancer tissues excised from a primary liver cancer patient through surgery, and can be subjected to in vitro long-term subculture. . The cell line has been passaged more than 100 times, the cells still grow rapidly, and the stem cell properties are maintained. The cell line expresses markers of various stem cells, has the self-renewal capacity of the stem cells, and has the potential of directional differentiation to different tumor cells; and has tumor properties, tumor forming ability and metastasis ability. The cell line is cultured in vitro for a long time without changing the property, and has strong tumorigenicity and metastatic capacity in an immunodeficient mouse.

Fixing the liver cancer stem cell (human tumor pluripotent stem cell) line T3A-A3 obtained by amplification culture with paraformaldehyde, immunizing common Balb/c mice 2-4 times a week, and each time is about 1 × 10⁷A cell. And (3) long-term immunization until the titer of the serum of the immunized mouse against a human liver cancer stem cell (human tumor pluripotent stem cell) line T3A-A3 is determined to be more than 1:50000 by adopting a conventional cellular immunochemical method. Taking mouse spleen cells and mouse myeloma cells SP2/0, and fusing to form hybridoma secreting mouse monoclonal antibody by a conventional PEG mediated fusion method. Preparing hybridoma monoclonals by a conventional methylcellulose plate method, respectively picking the monoclonals to a 96-well plate for continuous culture after the monoclonals grow, thereby obtaining a library of the hybridoma clones containing a large amount of monoclonal antibodies of anti-human liver cancer stem cells (human tumor pluripotent stem cells) line T3A-A3. Culture supernatants from each hybridoma clone in 96-well plates were collected for further assay and screening procedures.

Screening of anti-human tumor pluripotent stem cell mouse monoclonal antibody Hetumomab

Serum-free suspension medium was DMEM/F12(1:1) medium containing 20ng/mL EGF and 20ng/mL bFGF in a 1:50 ratio, supplemented with B27, 10ng/mL LIF, 2mmol/mL glutamine, and 1. mu.g/mL Heparin. Cells were washed 1 time before culture using serum-free medium. The human liver cancer stem cell (human tumor pluripotent stem cell) line T3A-A3 spherical cell cultured in serum-free suspension is gently blown into a single cell, and a 96-well plate is inoculated with 2000 cells/well. After culturing for 24h in serum-free medium, washing the cells with PBS containing 1% BSA, adding 100 μ L of culture supernatant of each hybridoma clone into each well, and incubating for 2h at room temperature; washing with PBS containing 1% BSA for 5 times, adding biotin-labeled anti-mouse secondary antibody, and reacting for 30min at room temperature; after washing 5 times with PBS containing 1% BSA, Cy 3-labeled Avidin was added and reacted at room temperature for 30 minutes; after washing 5 times with 1% BSA in PBS, the reaction between the monoclonal antibody hybridoma supernatants in the monoclonal antibody library and the human liver cancer stem cell (human tumor pluripotent stem cell) line T3A-A3 was determined by fluorescence microscopy under a fluorescence microscope. And (3) observing that partial cells are positively judged by fluorescent staining, and primarily screening to obtain the mouse monoclonal antibody capable of being combined with the surface antigen of the T3A-A3 membrane of the human liver cancer stem cell (human tumor pluripotent stem cell) line.

Further, spherical cells of serum-free suspension-cultured human liver cancer cell lines (Yan Li, Zhao-You Tang, Sheng-Long Ye, Yin-Kun Liu, Jie CHEN, Qiong Xue, Jun Chen, Dong-Mei Gao, Wei-Hua Bao.Estalich of cells with differential signaling from the metastic hepatocellular nuclear line MHCC97. J.world gastroenterology (English edition) 2001,7(05):630 and 636.) were selected, and the above procedure was performed in the same manner, and the supernatant of hybridoma monoclonal antibodies in the monoclonal antibody library was judged to react with the human liver cancer stem cells (MHCC97 spherical cells are rich in liver cancer 97L) to obtain mouse monoclonal antibodies capable of binding to the surface antigen of the stem cells.

Screening 1 strain of mouse monoclonal antibody Hetumomab from a mouse monoclonal antibody library of anti-human tumor pluripotent stem cells, wherein the mouse monoclonal antibody Hetumomab not only can be combined with the surface antigen of a membrane of a human tumor pluripotent stem cell line T3A-A3, but also can be combined with the surface antigen of a membrane of a stem cell (MHCC97L spherical cell) of a human liver cancer MHCC97L cell line, and the mouse monoclonal antibody Hetumomab can be proved to be capable of recognizing human liver cancer stem cells of different sources. The mouse monoclonal antibody Hetumomab was selected for further identification and pharmacodynamic studies.

Mouse hybridoma cells secreting Hetumomab monoclonal antibody are preserved in China general microbiological culture Collection center (CGMCC) at 2016, 3, 16 days (Beijing institute of microbiology, China academy of sciences, No. 3, Xilu No.1, Beicheng, the rising area) with the preservation number of CGMCC No. 12251.

The hybridoma cells were expanded and the antibody-containing supernatant was collected. The monoclonal antibody subclass detection kit of Southern Biotech company is adopted to identify the monoclonal antibody class and subclass and the ELISA secondary antibody of Sigma company is adopted to detect the antibody yield of the supernatant. The experimental result shows that the monoclonal antibody Hetumomab is IgG1 heavy chain and kappa light chain.

And (3) carrying out in-vitro amplification culture on the hybridoma cells secreting the monoclonal antibody Hetumomab, replacing a serum-free culture medium after the cells grow to 80%, and collecting the serum-free supernatant secreting the antibody after continuing to culture for 4-5 days. And (3) purifying the monoclonal antibody Hetumomab by using an anti-Protein G purification column. The purity of the isolated and purified Hetumomab monoclonal antibody was then identified by Coomassie blue staining using 10% SDS-PAGE. The results show that the molecular weight of the heavy chain of Hetumomab is about 47kDa and the molecular weight of the light chain is about 26kDa, which corresponds to the molecular weight of the theoretically normal IgG antibody heavy and light chains. The purity of the target band of the scanning analysis electrophoresis is more than 95 percent, and the purity of the purified Hetumomab monoclonal antibody meets the requirements of subsequent experiments.

Example 2 specific expression of Hetumomab target antigens in various tumor cells and tissues

The Hetumomab target antigen is expressed in various human liver cancer, lung cancer and gastric cancer cell lines and can be expressed on the surface of living cells.

The expression condition of the monoclonal antibody Hetumomab target antigen on the surface of living cells in various human tumor cell lines such as liver cancer, lung cancer and gastric cancer cell lines is detected by adopting a conventional living cell immunofluorescence staining technology. The specific technical method is as follows: cell slide or seed 96-well culture plate (4X 10)³One/well), when the cells grow to 60% -70% full, washing 2 times with serum-free culture solution and 1 time with PBS. Adding primary antibody (hybridoma supernatant or purified antibody), mouse anti-alpha-tublin antibody (dilution 1:1000) as cell permeation control, normal mouse IgG, SP2/0 supernatant, PBS as negative control, and incubating at room temperature for 1 h; viable cells were washed 5 times (5 min each time) with 1% BSA in PBS; fixing 4% paraformaldehyde at room temperature for 15 min; fixed cell washes (PBS with 0.1% BSA, 0.05% tween-20) were washed 5 times for 5min each; adding 100 μ l of secondary antibody, and incubating at room temperature in dark for 30 min; washing with fixed cell lotion for 5 times, each for 5 min; blocking 50. mu.l of PBS containing 10. mu.g/ml DAPI, 50% glycerol; and (4) observing under a fluorescence microscope. The result was positive when some cells were observed to show membrane fluorescent staining.

As a result, the monoclonal antibody Hetumomab target antigen can be expressed on the surfaces of the living cells of the following human liver cancer cell, lung cancer cell and gastric cancer cell lines (purchased from the cell resource center of the institute of basic medicine of Chinese academy of medical science):

human hepatoma cell lines: MHCC97L, Bel 7402-V13;

human lung cancer cell lines: a549 and SPCA-1;

human gastric cancer cell lines: SNU-5 and BGC-823.

A partial typical photograph of a positive result is shown in FIG. 1.

The above results show that the monoclonal antibody Hetumomab target antigen is expressed in various human tumors, such as liver cancer, lung cancer and gastric cancer cell lines, and can be expressed on the membrane surface of living cancer cells.

Secondly, the Hetumomab target antigen is specifically and highly expressed in human liver cancer, lung cancer and gastric cancer tissues.

The expression condition of the monoclonal antibody Hetumomab target antigen in a plurality of human liver cancer, lung cancer and gastric cancer patients and other related tissues is detected by adopting the traditional conventional immunohistochemical technology and taking Hetumomab as a primary antibody and adopting an anti-mouse antibody secondary antibody.

The specific technical method is as follows: dewaxing the tissue slices by a conventional method; pouring citric acid buffer solution (pH6.0) into the antigen repairing box, adding the sheet, placing the repairing box in boiling water, heating in water bath for 30min, and naturally cooling at room temperature for 2 hr; washing with PBS for 3min × 3 times, spin-drying the water on the slices, and immediately drawing circles along the tissues with a grouping pen; adding a drop of endogenous peroxidase blocking solution to each tissue, incubating at room temperature for 20min, washing with PBS for 3min × 3 times, discarding the washing solution, adding a drop of normal animal serum, i.e., goat serum, sealing, and incubating at room temperature for 20 min; removing the sealing serum, adding primary antibody to each tissue point, placing in a moisture preservation box, and incubating overnight at 4 ℃; removing the primary antibody, washing with PBS for 3min × 3 times, removing the washing solution, adding a secondary antibody, namely biotin-anti-mouse antibody, and incubating at room temperature for 20 min; washing with PBS for 3min × 5 times, removing the washing solution, adding a drop of Avidin-HRP, and incubating at room temperature for 10 min; washing with PBS for 3min × 3 times, removing the washing solution, adding a drop of freshly prepared DAB, observing under a microscope, and strictly timing, and stopping washing with tap water after the tissue is positive; counterstaining with hematoxylin for 5min, separating with 1% hydrochloric acid-75% alcohol for 2 s, and washing with tap water; after dehydrating the slices, sealing the slices with neutral gum and observing the slices under a microscope.

The monoclonal antibody Hetumomab is used as a primary antibody, and the expression conditions of the single-anti-Hetumomab target antigen in 120 cases of human liver cancer tissues, 20 cases of paracancerous tissues, 10 cases of normal liver tissues, 10 cases of hepatitis tissues and 40 cases of liver cirrhosis tissues are detected. The results of the experiment (table 1) show: the target antigen of the monoclonal antibody Hetumomab is specifically expressed in 79.17 percent (95/120) of human liver cancer tissues, but is not expressed in paracarcinoma tissues, normal liver tissues, hepatitis tissues and liver cirrhosis tissues, which indicates that the target antigen of the monoclonal antibody Hetumomab is specifically and highly expressed in the human liver cancer tissues.

TABLE 1 results of immunohistochemical detection of expression of Hetumomab target antigen in human hepatoma tissue

The monoclonal antibody Hetumomab is used as a primary antibody, and the expression conditions of the single-anti-Hetumomab target antigen in 160 cases of human lung cancer tissues, 32 cases of paracarcinoma tissues and 3 cases of normal lung tissues are detected. The results of the experiment (table 2) show: the target antigen of the monoclonal antibody Hetumomab is specifically expressed in 82.5 percent (132/160) of human lung cancer tissues, but is only expressed in 6.25 percent of paracarcinoma tissues, and the expression of the target antigen is not seen in normal lung tissues, which indicates that the target antigen of the monoclonal antibody Hetumomab is specifically and highly expressed in the human lung cancer tissues.

TABLE 2 immunohistochemical technique for detecting the expression of Hetumomab target antigen in human lung cancer tissues

The expression conditions of the single-anti-Hetumomab target antigen in 110 cases of human gastric cancer tissues, 20 cases of paracarcinoma tissues and 3 cases of normal gastric tissues are detected by taking the monoclonal antibody Hetumomab as a primary antibody. The results of the experiment (table 3) show: the target antigen of the monoclonal antibody Hetumomab is specifically expressed in 93.64 percent (103/110) of human gastric cancer tissues, but is not expressed in the tissues beside cancer and normal stomach, which indicates that the target antigen of the monoclonal antibody Hetumomab is specifically and highly expressed in the human gastric cancer tissues.

TABLE 3 results of immunohistochemical detection of expression of Hetumomab target antigen in human gastric cancer tissues

A typical partial positive photograph of the above immunohistochemical assay results is shown in FIG. 2.

These results indicate that the monoclonal antibody Hetumomab target antigen is specifically and highly expressed in cancer tissues of many human tumors, such as liver cancer, lung cancer and gastric cancer.

Example 3 monoclonal antibody Hetumomab recognition of tumor Stem cells

Firstly, cancer cells identified by the monoclonal antibody Hetumomab are enriched in sphere culture cells of the cancer cells.

According to The extensive literature, it has been reported that tumor stem cells can be enriched in serum-free suspension culture, i.e., sphere culture (Reynolds, B.A. and S.Weiss, "clone and position analysis systems purified that an EGF-responsive mammalia imaging system CNS therapy a stem cell," Dev Biol,1996.175(1): p.1-13.; Fang, N., et al, "pH responsive addition of phosphorus sensitive poly (acrylic acid) culture," colloid B Biol, 2005.42(3-4): p.245-52.; and titanium, V., thermal gradient) culture surface, "collagen Su B Biol s. 2005.42(3-4): p.245-52.; and cement culture of sphere culture of collagen-3. J.35. culture of culture medium J.133-3. c. (III). Therefore, whether the cells identified by the monoclonal antibody Hetumomab are related to the tumor stem cells or not is preliminarily judged according to whether the cancer cells identified by the monoclonal antibody Hetumomab can be enriched in sphere culture or not.

After 5 days of serum-free culture of the human hepatoma cell lines Bel7402-V13 and MHCC97L cells, the Hetumomab in parent cells and sphere culture cells is subjected to live cell flow cytometry⁺The cells were examined. The results of the experiment (Table 4) show that Hetumomab⁺The proportion of the cells in Bel7402-V13sphere cells is 8.92 percent, which is thinner than that in the parentThe proportion of cells is 2.25% enriched by 3.96 times; hetumomab⁺The proportion of cells in MHCC97L sphere cells was 7.51% enriched by a factor of 2.18 compared to 3.45% in the parental cells. That is, Hetumomab of the liver cancer cell line after serum-free culture⁺The cells are enriched.

Table 4. result of detecting enrichment of hepatoma cells identified by monoclonal antibody Hetumomab in sphere culture cells of hepatoma cells by immune flow type fluorescence technology

After 5 days of serum-free culture of SPCA-1 and A549 cells of human lung cancer cell lines, the Hetumomab in parental cells and sphere culture cells is subjected to flow cytometry⁺The cells were examined. The results of the experiment (Table 5) show that Hetumomab⁺The proportion of the cells in the SPCA-1sphere cells is 7.34 percent, which is 4.22 times more enriched than the proportion of the cells in the parent cells, which is 1.74 percent; hetumomab⁺The proportion of cells in A549sphere cells was 13.30%, which is 1.84-fold enriched compared to its proportion in parental cells of 7.23%. That is, Hetumomab of lung cancer cell lines after serum-free culture⁺The cells are enriched.

TABLE 5 results of detection of lung cancer cell enrichment in cultured lung cancer cell by monoclonal antibody Hetumomab by immunoflow fluorescence technology

After the SNU-5 and BGC-823 cells of the human gastric cancer cell line are cultured for 5 days in a serum-free manner, the Hetumomab in the parent cells and the cultured sphere cells is cultured by adopting the living cell flow type fluorescence technique⁺The cells were examined. The results of the experiment (Table 6) show that Hetumomab⁺The proportion of the cells in the SNU-5sphere cells is 7.19 percent, which is 2.13 times more enriched than the proportion of the cells in the parent cells which is 3.38 percent; hetumomab⁺The proportion of the cells in the BGC-823sphere cells was 7.45% compared with that in the parental cellsThe ratio of 2.95% was 2.53-fold enriched. That is, Hetumomab of gastric cancer cell lines after serum-free culture⁺The cells are enriched.

Table 6. result of detecting enrichment of gastric cancer cells identified by monoclonal antibody Hetumomab in sphere culture cells of gastric cancer cells by immune flow type fluorescence technology

A typical profile of a portion of the above results of the immuno-flow fluorescence assay is shown in FIG. 3.

These results show that the cancer cells identified by the monoclonal antibody Hetumomab are significantly enriched in the sphere culture cells of various human tumors, such as liver cancer, lung cancer and gastric cancer cell lines.

Secondly, monoclonal antibody Hetumomab recognizes ESA and CD90 positive tumor stem cells.

Several documents (Yamashita T, et al. EpCAMPositive hepatocellular area tumor-inducing cells with stem/promoter cell defects. gastroenterology, 2009,136(3): 1012. about.1024. and Yang ZF. identification of local and circulating cancer cells in human liver cell.about.2008, 47(3): 919. 928) demonstrate that ESA and CD90 are tumor stem cell surface markers for some hepatoma cells. In order to detect that the monoclonal antibody Hetumomab liver cancer recognizing cells in the human liver cancer cell Bel7402-V13 cells are liver cancer stem cells with positive ESA and CD90 markers, two-color flow fluorescence is adopted to stain the human liver cancer Bel7402-V13 cells cultured in a serum-free culture medium for 5 days.

The results (as in Table 7) show that the fraction of cells recognized by the monoclonal antibody Hetumomab is 8.52%, and the ESA⁺The expression ratio of the stem cells is 9.02 percent, the co-infection ratio of the stem cells and the stem cells is 3.63 percent, namely, 40.2 percent of ESA is identified by the monoclonal antibody Hetumomab⁺A stem cell. The results of staining MHCC97L cells of human liver cancer cultured for 5 days in serum-free medium (see Table 7) show that the proportion of cells recognized by monoclonal antibody Hetumomab is 2.38%, and the proportion of cells recognized by CD90 is⁺The expression ratio of the stem cells is 4.54 percent, and the co-staining ratio of the stem cells and the stem cells is 2.01%, i.e. 44.3% of CD90 was recognized by monoclonal antibody Hetumomab⁺A stem cell.

TABLE 7 results of two-color flow-type fluorescence detection of co-staining of monoclonal antibody Hetumomab, ESA and CD90 liver cancer stem cell surface markers in hepatoma cells

These results demonstrate that monoclonal antibody Hetumomab recognizes human tumor stem cells positive for markers such as ESA and CD 90.

The cancer cells identified by the monoclonal antibody Hetumomab have stronger self-renewal capacity

Self-renewal capacity, strong invasive capacity, chemotherapy drug resistance and strong tumorigenic capacity are important basic characteristics of tumor stem cells from common progeny tumor cells. Therefore, to further verify whether the cells recognized by the monoclonal antibody hetumab have tumor stem cell characteristics, hetumab among various human tumor cells was sorted⁺Cells tested for their self-renewal, invasion, drug resistance and tumorigenic capacity in vivo.

The self-renewal capacity of tumor stem cells is mainly expressed by the ability to form spheres in serum-free medium in a manner known as asymmetric division, i.e., when one cell divides into two daughter cells, one of the daughter cells retains the same characteristics as the parent cell, while the other daughter cell can continue to divide to form normal daughter cells. Thus, it is possible to detect Hetumomab⁺The self-renewal capacity of cells was determined by their ability to sphere in serum-free medium.

Adopts the flow sorting technology to separate Hetumomab from Bel7402-V13sphere cells of human liver cancer cultured for 5 days⁺Cell, parental cell and Hetumomab^-A cell. The sorted cells were inoculated at 500 cells/well into a semisolid sphere medium containing 0.8% methylcellulose (semisolid sphere medium containing 0.8% methylcellulose, 20ng/mL EGF and 20ng/mL bFGF), and B27, 10ng/mL LIF, 2mmol/mL glutamine and 1u/mL Heparin in a 1:50 ratio, DMEM/F12(1:1) culture solution) In the above-mentioned method, the cells were cultured in an ultra-low adhesion 24-well plate, and the number of spherulites of each cell was observed. The results show (Table 8), Hetumomab⁺Cell, parental cell and Hetumomab^-The balling numbers of the cells under the serum-free culture medium condition are 262 +/-8.5, 168 +/-5.6 and 98 +/-5.6 respectively, namely Hetumomab⁺The balling rate of the cells is obviously higher than that of other two cells (p)<0.05). Thus, Hetumomab⁺Cell-specific parental cell and Hetumomab^-The cells have a stronger self-renewal capacity.

TABLE 8 Hetumomab in hepatoma cells⁺Cell, parental cell and Hetumomab^-Comparison of cell self-renewal Capacity

Separating Hetumomab from SPCA-1sphere cells of human lung cancer by adopting flow sorting technology⁺Cell, parental cell and Hetumomab^-A cell. The sorted cells were seeded at 500 cells/well in semisolid sphere medium containing 0.8% methylcellulose, cultured in ultra low adhesion 24-well plate, and the number of spheroids of each cell was observed. The results show (Table 8), Hetumomab⁺Cell, parental cell and Hetumomab^-The cell balling numbers under the condition of serum-free culture medium are 165.7 +/-6.0, 127 +/-5.6 and 83.7 +/-4.7 respectively, namely Hetumomab⁺The balling rate of the cells is obviously higher than that of other two cells (p)<0.05). Thus, Hetumomab⁺Cell-specific parental cell and Hetumomab^-The cells have a stronger self-renewal capacity.

TABLE 9 Hetumomab in Lung cancer cells⁺Cell, parental cell and Hetumomab^-Comparison of cell self-renewal Capacity

Hetumomab is separated from SNU-5sphere cells of human gastric cancer by adopting flow sorting technology⁺Cell, parental cell and Hetumomab^-A cell. Will be provided withThe sorted cells were seeded at 500 cells/well in semisolid sphere medium containing 0.8% methylcellulose, cultured in ultra low adhesion 24-well plate plates, and the number of spheroids of each cell was observed. The results show (Table 10), Hetumomab⁺Cell, parental cell and Hetumomab^-The cell balling numbers under the condition of serum-free culture medium are respectively 24 +/-1.4, 15.5 +/-0.7 and 11.5 +/-0.7, namely Hetumomab⁺The balling rate of the cells is obviously higher than that of other two cells (p)<0.05). Thus, Hetumomab⁺Cell-specific parental cell and Hetumomab^-The cells have a stronger self-renewal capacity.

TABLE 10 Hetumomab in gastric cancer cells⁺Cell, parental cell and Hetumomab^-Comparison of cell self-renewal Capacity

These results show that the various cancer cells (liver cancer, lung cancer and gastric cancer) identified by the monoclonal antibody Hetumomab have stronger self-renewal capacity, namely, one of the main characteristics of the tumor stem cells: high self-renewal capacity.

Cancer cells identified by monoclonal antibody Hetumomab have stronger invasive ability

Adopts the flow sorting technology to separate Hetumomab from Bel7402-V13sphere cells of human liver cancer cultured for 5 days⁺Cell, parental cell and Hetumomab^-A cell. The sorted cells were seeded in equal numbers in a Transwell chamber pre-coated with Matrigel gel, fixed after 24h, and the number of membrane-penetrating cells was observed under a microscope. The results show (Table 11), Hetumomab⁺Cell and relativeThe present cell and Hetumomab^-The number of cells penetrating the membrane is 308 + -9.5, 210 + -10.7 and 132 + -14.7 per field, respectively, i.e. Hetumomab⁺The number of cells invading through the membrane is significantly higher than the other two (p)<0.05). Thus, Hetumomab⁺Cell-specific parental cell and Hetumomab^-The cells have stronger invasive ability.

TABLE 11 Hetumomab in hepatoma cells⁺Cell, parental cell and Hetumomab^-Comparison of cell invasiveness

Separating Hetumomab from cultured human lung cancer SPCA-1sphere cells by adopting flow sorting technology⁺Cell, parental cell and Hetumomab^-A cell. The sorted cells were seeded in equal numbers in a Transwell chamber pre-coated with Matrigel gel, fixed after 24h, and the number of membrane-penetrating cells was observed under a microscope. The results show (Table 12), Hetumomab⁺Cell, parental cell and Hetumomab^-The number of the cells penetrating the membrane is respectively 222 +/-11.5, 193.7 +/-5.7 and 154.3 +/-12.1 per visual field, namely Hetumomab⁺The number of cells invading through the membrane is significantly higher than the other two (p)<0.05). Thus, Hetumomab⁺Cell-specific parental cell and Hetumomab^-The cells have stronger invasive ability.

TABLE 12 Hetumomab in Lung cancer cells⁺Cell, parental cell and Hetumomab^-Comparison of cell invasiveness

Hetumomab is separated from cultured SNU-5sphere cells of human gastric cancer by adopting flow sorting technology⁺CellsParent cell and Hetumomab^-A cell. The sorted cells were seeded in equal numbers in a Transwell chamber pre-coated with Matrigel gel, fixed after 24h, and the number of membrane-penetrating cells was observed under a microscope. The results show (Table 13), Hetumomab⁺Cell, parental cell and Hetumomab^-The number of cells penetrating the membrane is 247.5 + -19.1, 142.5 + -9.2 and 145.0 + -11.3 per field, respectively, that is, Hetumomab⁺The number of cells invading through the membrane is significantly higher than the other two (p)<0.05). Thus, Hetumomab⁺Cell-specific parental cell and Hetumomab^-The cells have stronger invasive ability.

TABLE 13 Hetumomab in gastric cancer cells⁺Cell, parental cell and Hetumomab^-Comparison of cell invasiveness

These results show that the various cancer cells (liver cancer, lung cancer and gastric cancer) identified by the monoclonal antibody Hetumomab have stronger invasive ability, namely, one of the main characteristics of the tumor stem cells: high invasiveness.

The cancer cells identified by the monoclonal antibody Hetumomab have stronger capacity of resisting chemotherapeutic drugs

For detecting Hetumomab⁺The drug resistance of the hepatoma cells, namely Hetumomab obtained by the cell flow sorting of human hepatoma cells Bel7402-V13sphere⁺Cell, parental cell and Hetumomab^-The cells were plated in 96-well plates at 5000 cells/well, with each set of cells containing 0. mu.g/mL, 0.0625. mu.g/mL, 0.125. mu.g/mL, 0.25. mu.g/mL, 0.5. mu.g/mL, 1. mu.g/mL2. mu.g/mL and 4. mu.g/mL of cisplatin 8 different concentrations in complete medium, the medium was changed 1 time after 3 days, and after 7 days, OD was measured by the CCK8 method to determine IC50 which reflects the drug resistance. The results of the experiments show (Table 14, FIG. 4), Hetumomab⁺Cell, parental cell and Hetumomab^-The IC50 of the cells were 0.739. mu.g/mL, 0.502. mu.g/mL and 0.313. mu.g/mL, respectively, i.e., Hetumomab⁺The drug resistance of the cell is obviously higher than that of the parental cell and the Hetumomab^-Cellular, statistical significance of the differences (p)<0.05). Thus, Hetumomab⁺Hepatoma cells than parental cells and Hetumomab^-The cells have a greater ability to tolerate chemotherapeutic drugs.

TABLE 14 Hetumomab in hepatoma cells⁺Cell, parental cell and Hetumomab^-Comparison of the ability of cells to tolerate chemotherapeutic drugs

For detecting Hetumomab⁺Drug resistance of lung cancer cells, Hetumomab obtained by flow sorting of SPCA-1sphere cells of human lung cancer cells⁺Cell, parental cell and Hetumomab^-Cells were seeded in 96-well plates at 5000 cells/well, and each group of cells was cultured in complete media containing 7 different concentrations of cisplatin, 0. mu.g/mL, 0.2. mu.g/mL, 0.4. mu.g/mL, 0.6. mu.g/mL, 0.8. mu.g/mL, 1. mu.g/mL and 2. mu.g/mL, after which IC50 reflecting its drug resistance was determined by measuring OD by the CCK8 method. The results of the experiments show (Table 15, FIG. 4), Hetumomab⁺Cell, parental cell and Hetumomab^-The IC50 of the cells was 0.707. mu.g/mL, 0.513. mu.g/mL, and 0.180. mu.g/mL, respectively, i.e., Hetumomab⁺The drug resistance of the cell is obviously higher than that of the parental cell and the Hetumomab^-Cellular, statistical significance of the differences (p)<0.05). Thus, Hetumomab⁺Lung cancer cell than parental cell and Hetumomab^-The cells have a greater ability to tolerate chemotherapeutic drugs.

TABLE 15 Hetumomab in Lung cancer cells⁺Cell, parental cell and Hetumomab^-Comparison of the ability of cells to tolerate chemotherapeutic drugs

For detecting Hetumomab⁺Drug resistance of gastric cancer cells, namely Hetumomab obtained by flow sorting of SNU-5sphere cells of human gastric cancer cells⁺Cell, parental cell and Hetumomab^-Cells were seeded in 96-well plates at 5000 cells/well, and each group of cells was cultured in complete media containing 8 different concentrations of cisplatin, 0. mu.g/mL, 0.0125. mu.g/mL, 0.025. mu.g/mL, 0.05. mu.g/mL, 0.1. mu.g/mL, 0.2. mu.g/mL, 0.4. mu.g/mL and 0.8. mu.g/mL, after which IC50 reflecting its drug resistance was determined by measuring OD by the CCK8 method. The results of the experiments show (Table 16, FIG. 4), Hetumomab⁺Cell, parental cell and Hetumomab^-The IC50 of the cells were 0.285. mu. mol/L, 0.155. mu. mol/L and 0.094. mu. mol/L, respectively, i.e., Hetumomab⁺The drug resistance of the cell is obviously higher than that of the parental cell and the Hetumomab^-Cellular, statistical significance of the differences (p)<0.05). Thus, Hetumomab⁺Stomach cancer cell than parental cell and Hetumomab^-The cells have a greater ability to tolerate chemotherapeutic drugs.

TABLE 16 Hetumomab in gastric cancer cells⁺Cell, parental cell and Hetumomab^-Comparison of the ability of cells to tolerate chemotherapeutic drugs

These results show that various cancer cells (liver cancer, lung cancer and gastric cancer) identified by the monoclonal antibody Hetumomab have stronger capacity of resisting chemotherapeutic drugs, namely, one of the main characteristics of tumor stem cells: strong drug resistance.

Sixthly, the cancer cells recognized by the monoclonal antibody Hetumomab have stronger in-vivo tumorigenic capacity

The "gold standard" to test whether a cell is a tumor stem cell is strongly tumorigenic in vivo. Therefore, Hetumomab obtained by cell flow sorting of human hepatoma cells Bel7402-V13sphere⁺Cell, parental cell and Hetumomab^-The cells were inoculated subcutaneously into 4-week-old nude mice, and the tumors were observed in vivo for a long period of time. The results are shown in Table 17, 1X 10⁴Hetumomab⁺Cells were tumorigenic in mice 3 weeks after inoculation, whereas parental cells required 1X 10⁵The individual cells developed tumors, Hetumomab, 3 weeks after inoculation^-Cells did not develop tumors throughout the observation period. The results of this classical experiment demonstrate that Hetumomab⁺The in vivo tumorigenicity of the cells is higher than that of the parent and the Hetumomab^-The cells were significantly strong. Indication of Hetumomab⁺The cells have the high tumorigenicity characteristics of tumor stem cells and meet the judgment 'gold standard' of the tumor stem cells. Therefore, the cells recognized by the monoclonal antibody Hetumomab are liver cancer tumor stem cells.

TABLE 17 Hetumomab in hepatoma cells Bel7402-V13⁺Cell, parental cell and Hetumomab^-Comparison of in vivo tumorigenic Capacity of cells (number of tumorigenic animals)

Hetumomab obtained by carrying out flow sorting on human gastric cancer cell SNU-5sphere cells⁺Cell, parental cell and Hetumomab^-The cells were inoculated subcutaneously into 4-week-old nude mice, and the tumors were observed in vivo for a long period of time. The results are shown in Table 18, 2X 10³Hetumomab⁺Cells can be inoculated in mice 3 monthsThe inner half of mice were tumorigenic, while the parental cells were 2X 10³The individual cells did not develop a tumor, Hetumomab, after 4 months of inoculation^-Cells did not develop tumors throughout the observation period. The results of this classical experiment demonstrate that Hetumomab⁺The in vivo tumorigenicity of the cells is higher than that of the parent and the Hetumomab^-The cells were significantly strong. Indication of Hetumomab⁺The cells have the high tumorigenicity characteristics of tumor stem cells and meet the judgment 'gold standard' of the tumor stem cells. Therefore, the cells recognized by the monoclonal antibody Hetumomab are gastric cancer tumor stem cells.

TABLE 18 Hetumomab in stomach cancer cells SNU-5⁺Cell, parental cell and Hetumomab^-Comparison of in vivo tumorigenic Capacity of cells (number of tumorigenic animals)

These results show that many cancer cells (liver cancer and gastric cancer) identified by monoclonal antibody Hetumomab have stronger in vivo tumorigenicity, namely, the cells have high tumorigenicity which is one of the main characteristics of tumor stem cells.

Example 4 monoclonal antibody Hetumomab can inhibit the self-renewal, invasion and drug resistance functions of tumor stem cells

The monoclonal antibody Hetumomab can obviously inhibit the self-renewal capacity (one of the main characteristics of tumor stem cells) of the tumor stem cells of various tumors (liver cancer, gastric cancer and lung cancer).

The self-renewal capacity of tumor stem cells is mainly expressed by the ability to form spheres in serum-free medium in a manner known as asymmetric division, i.e., when one cell divides into two daughter cells, one of the daughter cells retains the same characteristics as the parent cell, while the other daughter cell can continue to divide to form normal daughter cells. To demonstrate whether the monoclonal antibody Hetumomab is a functional monoclonal antibody capable of directly inhibiting liver cancer stem cells, purified monoclonal antibody Hetumomab (250 μ g/mL) is used as an experimental group and PBS is used as a negative control group after human liver cancer cell line Bel7402-V13sphere cells cultured in serum-free medium for 5 days are prepared into single cell suspension, and the cells are incubated for 2 hours at 37 ℃ during the period of time, and the cells and the antibody or the negative control are mixed uniformly every half hour. 500 cells of each group are inoculated in a semi-solid sphere culture medium (containing EGF, LIF, bFGF and the like) containing 0.8 percent of methyl cellulose, cultured in a plate with 24 holes and ultra-low adhesion, and the liquid is supplemented by 1 to 1.5mL every other day, and the cell balling quantity of the two groups is observed after 14 days. The experimental results (FIG. 5) show that the number of beads in the experimental group was 212. + -. 2.8, while the number of beads in the negative control group was 278.5. + -. 0.7, which is significantly higher than that in the experimental group. The spheronization inhibition rate of the monoclonal antibody Hetumomab to Bel7402-V13 cells reaches 23.9 percent, p is less than 0.05, and the statistical difference exists. The result shows that the monoclonal antibody Hetumomab can directly act on the liver cancer stem cells and inhibit the self-renewal capacity of the liver cancer stem cells.

In order to prove whether the monoclonal antibody Hetumomab is a functional monoclonal antibody capable of directly inhibiting the lung cancer stem cells, the inhibition effect of the monoclonal antibody Hetumomab on the SPCA-1 spheroidisation of a human lung cancer cell line is detected by the same method. The results (see Table 19 and FIG. 5) show that the numbers of spheroids in the experimental group with the highest antibody concentration are 88.3 + -7.2, while the numbers of spheroids in the negative control group are 151.3 + -9.1, which are significantly higher than those in the experimental group, the spheroids inhibition rate of the monoclonal antibody Hetumomab on SPCA-1 cells reaches 41.6%, and p is less than 0.01, which is statistically different. The result shows that the monoclonal antibody Hetumomab can directly act on the lung cancer stem cells and inhibit the self-renewal capacity of the lung cancer stem cells.

Table 19 shows the results of detecting that the monoclonal antibody Hetumomab inhibits the SPCA-1 balling of the human lung cancer cell line

In order to prove whether the monoclonal antibody Hetumomab is a functional monoclonal antibody capable of directly inhibiting the gastric cancer stem cells, the inhibition effect of the monoclonal antibody Hetumomab on the tumor stem cell subpopulation of CD44 positive cells of the human gastric cancer cell line SNU-5 is detected by the same method. The results (see Table 20, FIG. 5) show that the spheronization numbers of the experimental group with the highest antibody concentration are 18. + -. 2.0, while those of the negative control group are 128. + -. 4.0, which is significantly higher than that of the experimental group, in which the monoclonal antibody Hetumomab is used for SNU-5 CD44⁺The cell balling inhibition rate reaches 85.9 percent, p<0.01, there was a statistical difference. The results show that the method has the advantages of high yield,the monoclonal antibody Hetumomab can directly act on the gastric cancer stem cells and inhibit the self-renewal capacity of the gastric cancer stem cells.

TABLE 20 detection of monoclonal antibody Hetumomab inhibition of human gastric cancer cell line SNU-5 CD44⁺Results of cell balling

The results show that the monoclonal antibody Hetumomab can directly and obviously inhibit the self-renewal capacity of various cancer cells (liver cancer, lung cancer and gastric cancer), and the monoclonal antibody Hetumomab can not only identify the target tumor stem cells, but also can directly inhibit the functional (therapeutic) anti-tumor stem cell monoclonal antibody of the tumor stem cells.

Secondly, the monoclonal antibody Hetumomab can obviously inhibit the invasion capacity of tumor stem cells of various tumors (liver cancer, gastric cancer and lung cancer) (the second main characteristic of the tumor stem cells).

High invasiveness is another important biological characteristic of tumor stem cells. In order to prove whether the monoclonal antibody Hetumomab is a functional monoclonal antibody capable of directly inhibiting the liver cancer stem cells, the invasion capacity of the monoclonal antibody Hetumomab in inhibiting the liver cancer cells is analyzed by adopting a Transwell invasion experiment. The experimental results showed (FIG. 6) that the number of invaded cells in the PBS negative control group was (301.0. + -. 16.3)/visual field, and the number of invaded cells in the monoclonal antibody Hetumomab (0.5mg/ml) group was (148. + -. 16.4)/visual field. Experimental results show that the cell invasion ability of the monoclonal antibody Hetumomab after direct action is obviously weakened, the inhibition rate of the monoclonal antibody to the invasion of Bel7402-V13sphere cells is 50.8%, p is less than 0.05, and statistical differences exist. The experimental result shows that the monoclonal antibody Hetumomab can directly act on the liver cancer stem cells and inhibit the invasion capacity of the liver cancer stem cells.

In order to prove whether the monoclonal antibody Hetumomab is a functional monoclonal antibody capable of directly inhibiting the lung cancer stem cells, the inhibition effect of the monoclonal antibody Hetumomab on the invasion of the human lung cancer cell line SPCA-1 is detected by the same method. The experimental results showed (table 21, fig. 6) that the number of invaded cells in the PBS negative control group was (232.3 ± 3.1)/visual field, and the minimum number of invaded cells in the mab Hetumomab group was (153.0 ± 6.1)/visual field. Experimental results show that the cell invasion ability of the monoclonal antibody Hetumomab after direct action is obviously weakened, the inhibition rate of the monoclonal antibody to the SPCA-1sphere cell invasion is 34.1%, p is less than 0.05, and statistical differences exist. The experimental result shows that the monoclonal antibody Hetumomab can directly act on the lung cancer stem cells and inhibit the invasion capacity of the lung cancer stem cells.

TABLE 21 results of detecting inhibition of monoclonal antibody Hetumomab against SPCA-1 invasion of human lung cancer cell line

In order to prove whether the monoclonal antibody Hetumomab is a functional monoclonal antibody capable of directly inhibiting the gastric cancer stem cells, the inhibition effect of the monoclonal antibody Hetumomab on the invasion of the CD44 positive cell tumor stem cell subpopulation of the human gastric cancer cell line SNU-5 is detected by the same method. The experimental results showed (table 22, fig. 6) that the number of invaded cells in the PBS negative control group was (231 ± 7.0)/visual field, and the lowest number of invaded cells in the mab Hetumomab group was (56 ± 4.0)/visual field. The experimental result shows that the cell invasion ability of the monoclonal antibody Hetumomab after direct action is obviously weakened, and the monoclonal antibody is used for SNU-5 CD44⁺The inhibition rate of cell invasion was 75.8%, p<0.05, there was a statistical difference. The experimental result shows that the monoclonal antibody Hetumomab can directly act on the gastric cancer stem cells and inhibit the invasion capacity of the gastric cancer stem cells.

TABLE 21 detection of monoclonal antibody Hetumomab inhibition of human gastric cancer cell line SNU-5 CD44⁺Results of cell invasion

The results show that the monoclonal antibody Hetumomab can directly and obviously inhibit the invasion capacity of various cancer cells (liver cancer, lung cancer and gastric cancer), and the monoclonal antibody Hetumomab can not only identify the target tumor stem cells, but also can directly inhibit the functional (therapeutic) anti-tumor stem cell monoclonal antibody of the tumor stem cells.

And thirdly, the monoclonal antibody Hetumomab remarkably inhibits the capacity of the tumor stem cells of various tumors (liver cancer and lung cancer) to resist chemotherapeutic drugs (the third main characteristic of the tumor stem cells).

Drug resistance is one of the biological characteristics of tumor stem cells. To confirm whether the monoclonal antibody Hetumomab is a functional monoclonal antibody capable of directly inhibiting liver cancer stem cells, Bel7402-V13sphere cells cultured for 5 days were seeded in 96-well plates in a number of 5000 cells/well, and purified monoclonal antibody Hetumomab (0.5mg/mL) and PBS were added to each well at 37 ℃ with 5% CO₂After 24h incubation in the incubator, the antibody-containing medium was removed and cells were cultured in each set with medium containing cisplatin at a total concentration of 9 different concentrations of 0, 0.0625, 0.125, 0.25, 0.5, 1, 2, 4 and 8 μ g/mL for each set of cells, 1 change of cisplatin-containing complete medium was made for 48h, CCK-8 reagent was added 5 days later according to CCK-8 kit instructions, OD450 absorbance values were measured and IC50 values were calculated for each set. The experimental results show (figure 7) that the drug resistance of the cells directly acted by the monoclonal antibody Hetumomab is obviously reduced, the IC50 value of the cells is 0.334 mu g/mL, the IC50 value of the control group is 0.9 mu g/mL, and the drug resistance of the cells directly acted by the monoclonal antibody Hetumomab is obviously lower than that of the control group. The experimental result shows that the monoclonal antibody Hetumomab can directly act on the liver cancer stem cells and inhibit the drug resistance capability of the liver cancer stem cells.

In order to prove whether the monoclonal antibody Hetumomab is a functional monoclonal antibody capable of directly inhibiting the lung cancer stem cells, the inhibition effect of the monoclonal antibody Hetumomab on the drug resistance of the human lung cancer cell line SPCA-1 is detected by the same method. The experimental results show (figure 7) that the drug resistance of the cells directly acted by the monoclonal antibody Hetumomab is obviously reduced, the IC50 value of the cells is 0.136 mu g/mL at the lowest, the IC50 value of the control group is 0.351 mu g/mL, and the drug resistance of the cells directly acted by the monoclonal antibody Hetumomab is obviously lower than that of the control group. The experimental result shows that the monoclonal antibody Hetumomab can directly act on the lung cancer stem cells and inhibit the drug resistance capability of the lung cancer stem cells.

The results show that the monoclonal antibody Hetumomab can directly and obviously inhibit the capacity of various cancer cells (liver cancer and lung cancer) for tolerating chemotherapeutic drugs, and the monoclonal antibody Hetumomab can not only identify the target tumor stem cells, but also can directly inhibit the functional (therapeutic) antitumor stem cell monoclonal antibody of the tumor stem cells.

Example 5 monoclonal antibody Hetumomab has pharmacodynamic effects of inhibiting tumor transplantation tumor growth and coordinating chemotherapy in animals

The previous series of experimental results prove that the monoclonal antibody Hetumomab is a monoclonal antibody targeting multiple tumor stem cells; and in vitro pharmacodynamic research results show that the monoclonal antibody Hetumomab can obviously inhibit the self-renewal, invasion and drug resistance of various tumor stem cells. In order to further clarify the influence of the monoclonal antibody Hetumomab on the growth, the metastasis and the drug resistance of various tumors in vivo, various human tumor animal models are adopted to evaluate the pharmacodynamic action of the monoclonal antibody Hetumomab on the growth, the metastasis and the drug resistance of various tumors in vivo.

The monoclonal antibody Hetumomab can obviously inhibit the growth of human liver cancer transplantable tumor in vivo, can obviously and synergistically enhance the curative effect of chemotherapy, obviously prolongs the life cycle, and has obvious antitumor effect.

The experimental study of the monoclonal antibody Hetumomab on treating the liver cancer in nude mice is carried out, the curative effects of the monoclonal antibody alone, the chemotherapeutic drug alone and the monoclonal antibody combined with the chemotherapeutic drug on treating the liver cancer are observed and compared, whether the monoclonal antibody Hetumomab can treat the growth and the drug resistance of the liver cancer in vivo is analyzed and compared, and the optimal scheme of treating the liver cancer by targeting liver cancer stem cells is discussed.

The sphere cells of Bel7402-V13 were inoculated subcutaneously into nude mice at 3 ten thousand/mouse, and the nude mice were randomly divided into 6 groups (6/group), which were: a single chemotherapeutic drug group (cisplatin 0.3mg/kg, 6); antibody high dose group (10mg/kg, 6); antibody high dose + chemotherapy group (6); antibody low dose group (2.5mg/kg, 6); antibody low dose + chemotherapy group (6); PBS group (6). Treatment started the day after cell inoculation, 2 times per week and ended after 5 weeks. The long diameter and short diameter of the subcutaneously transplanted tumor were measured 2 times per week, and the tumor volume was calculated by the formula V ═ (pi/6) × (long diameter × short diameter), and changes in tumor volume and growth rate were observed for each group. After drug withdrawal, the growth of the transplanted tumors was continued and tumor size was recorded. Tumor growth rate ═ V_t-V₀) Number of days, V_tIs the tumor volume at each measurement, V₀Is the tumor volume (V) before administration₀Refers to the tumor volume at which dosing was stopped).

The growth curve of the transplanted tumor in the mouse is shown in fig. 8, and the monoclonal antibody Hetumomab can obviously inhibit the growth of the transplanted tumor in the nude mouse. And the inhibition rate of the transplanted tumor is gradually increased along with the increase of the dosage of the antibody, so that a dosage dependence relationship exists. The experimental result is shown in fig. 9, when the treatment is stopped for 5 weeks, the inhibition rates of the high-dose and low-dose monoclonal antibodies Hetumomab on the transplanted tumors are 71.5% and 54.4% respectively, the inhibition rate of the chemotherapeutic drug group is 83.5%, and the inhibition rates of the high-dose and low-dose monoclonal antibodies combined with the chemotherapeutic drug group are similar and reach about 97%. The results suggest that the monoclonal antibody combined with the chemotherapeutic drug group shows better treatment effect in the treatment of the transplanted tumor compared with the monoclonal antibody and the chemotherapeutic drug group which are used singly.

Mice died when the drug was discontinued for one month. The inhibition rates of the groups are shown in fig. 10, the inhibition rates of the monoclonal antibody on the transplanted tumor are 49.1% and 34.4% respectively, the inhibition rates of the monoclonal antibody on the combined chemotherapeutic drug are similar and about 84.5%, but the inhibition rate of the single chemotherapeutic drug is 48.6%. The results suggest that the inhibition rate of the monoclonal antibody and the chemotherapeutic drug combination group on the mouse transplantation tumor is higher than that of other treatment scheme groups, P<0.05, the difference was statistically significant. Compared with the volume of the transplanted tumor at the time of drug withdrawal for one month after drug withdrawal, the tumor growth rate of the monoclonal antibody combined chemotherapy group is 0.051cm³Tumor growth rate/day, compared to PBS control group (0.239 cm)³Day) 4.7 times lower and tumor growth rate (0.148 cm) higher than that of monoclonal antibody, low dose group and chemotherapeutic group³0.185 cm/day³Daily and 0.154cm³Day) by 2.9, 3.6 and 3.1 times respectively, and the results show that the method of combining the monoclonal antibody with the chemotherapeutic drug can effectively inhibit the growth and the recurrence of the tumor.

The entire treatment and observation process lasted six months. The survival curves of the mice over six months (fig. 11) showed that the difference in the survival curves of these 6 groups of mice was statistically significant, with P < 0.05. The survival condition of the mouse of the monoclonal antibody and the chemotherapeutic drug combination is obviously better than that of the PBS control group, the monoclonal antibody group and the chemotherapeutic drug combination. It is suggested that the life of mice can be prolonged by the combination of monoclonal antibody and chemotherapy drug.

The results show that the monoclonal antibody Hetumomab can obviously inhibit the growth of the human liver cancer transplantation tumor by being used alone, and has obvious pharmacodynamic action of inhibiting liver cancer. The monoclonal antibody Hetumomab combined with the chemotherapeutic drug group shows high inhibition rate on transplanted tumors, can obviously inhibit the growth of tumors, has better treatment effect than the single antibody group and the single chemotherapeutic drug group, and shows that the monoclonal antibody combined with the chemotherapeutic drug can effectively inhibit the growth of tumors and reduce the chemotherapy resistance. The survival time of the mice of the combined medicine group is obviously longer than that of the mice of the single antibody group and the single chemotherapeutic medicine group, which shows that the scheme of treating the tumors by using the monoclonal antibody Hetumomab and the chemotherapeutic medicine can not only treat the growth of the tumors, but also prolong the survival time of the mice, and possibly obviously inhibit the death of the mice caused by the metastasis of the tumors in vivo.

Secondly, the monoclonal antibody Hetumomab can obviously inhibit the growth of the human lung cancer transplantation tumor in vivo and has obvious antitumor effect.

The experimental study of the monoclonal antibody Hetumomab on the treatment of the lung cancer in nude mice is carried out, and the curative effect of treating the lung cancer by using the monoclonal antibody and the chemotherapeutic drug alone is observed.

Specifically, spheroid cells of the human lung cancer cell line SPCA-1 were harvested at 2.5X 10⁵One cell/one inoculated nude mouse. 5 groups are divided, and each group comprises 5: PBS control group, chemotherapy group alone (cisplatin 0.3mg/kg), Hetumomab antibody high dose group (40mg/kg), Hetumomab antibody medium dose group (10mg/kg), Hetumomab low dose group (2.5 mg/kg). The antibody treatment is started on the 2nd day after the lung cancer cell inoculation, and the experimental group and the control group are treated by intraperitoneal injection, and the medicine is stopped after the treatment is carried out for 28 days after the lung cancer cell inoculation. Chemotherapeutic treatments were performed 2 times per week. The long diameter and short diameter of the subcutaneous graft tumor were measured 2 times per week, and the tumor volume was calculated, formula V ═ pi/6) × (long diameter × short diameter). The groups were observed for changes in tumor volume.

When the treatment was stopped 28 days after the inoculation, the tumor volume growth of the mice was observed and measured, and the inhibition rate was calculated. The growth curve of the transplanted tumor in the mouse is shown in fig. 12, the tumor volume inhibition rate is shown in table 22, the monoclonal antibody Hetumomab can significantly inhibit the growth of the transplanted tumor in the nude mouse, the inhibition rate of the transplanted tumor is correspondingly increased along with the increase of the dosage of the antibody, the inhibition rates of the monoclonal antibody Hetumomab with high, medium and low dosages on the transplanted tumor are respectively 55.97%, 43.56% and 35.58% when the drug is stopped, and the inhibition rate of the chemotherapeutic drug is only 24.91%.

The results show that the monoclonal antibody Hetumomab can obviously inhibit the growth of the human lung cancer transplantable tumor by being used alone, and has obvious pharmacodynamic action of inhibiting the lung cancer.

TABLE 22 results of monoclonal antibody Hetumomab inhibition of human lung carcinoma SPCA-1 graft tumor growth in animals

And thirdly, the monoclonal antibody Hetumomab can obviously inhibit the growth of the human gastric cancer transplantation tumor in vivo, can obviously and synergistically enhance the curative effect of chemotherapy, and has obvious antitumor effect.

The experimental study of the monoclonal antibody Hetumomab on the treatment of the gastric cancer in nude mice is carried out, the curative effects of the monoclonal antibody alone, the chemotherapeutic drug alone and the monoclonal antibody combined with the chemotherapeutic drug on the treatment of the gastric cancer are observed and compared, whether the monoclonal antibody Hetumomab can treat the growth of the gastric cancer in vivo and the chemotherapeutic curative effect is synergistically enhanced is analyzed and compared.

Specifically, spheroid cells of the human gastric cancer cell line SNU-5-V13 were harvested at 2.5X 10⁵One cell/one inoculated nude mouse. 7 groups are divided, and each group comprises 8: PBS control group, mouse IgG control group, chemotherapy group alone (cisplatin 0.3mg/kg), Hetumomab antibody high dose group (20mg/kg), Hetumomab low dose group (1.25mg/kg), Hetumomab high dose + chemotherapeutic agent, Hetumomab low dose + chemotherapeutic agent. Antibody treatment was started on day 2 after inoculation of gastric cancer cells, and the experimental group and the control group were treated by intraperitoneal injection, and after one month of treatment, the drug was stopped. The chemotherapeutic agent is administered for 4 weeks, and is discontinued 2 times per week. The long diameter and short diameter of the subcutaneous graft tumor were measured 2 times per week, and the tumor volume was calculated, formula V ═ pi/6) × (long diameter × short diameter). The groups were observed for changes in tumor volume.

When the treatment was stopped one month after the inoculation, the tumor volume growth of the mice was observed and measured, and the inhibition rate was calculated. The growth curve of the transplanted tumor in the mouse is shown in fig. 13, the tumor volume inhibition rate is shown in table 23, the monoclonal antibody Hetumomab can obviously inhibit the growth of the transplanted tumor in the nude mouse, the inhibition rate of the transplanted tumor is correspondingly increased along with the increase of the dosage of the antibody, the inhibition rates of the monoclonal antibody Hetumomab with high and low dosages on the transplanted tumor are 57.62% and 30.68% respectively when the drug is stopped, the inhibition rate of the chemotherapeutic drug is only 33.16%, the inhibition rates of the monoclonal antibody Hetumomab with high and low dosages combined with the chemotherapeutic drug respectively reach 70.68% and 47.93%, and the result shows that the inhibition rate of the monoclonal antibody combined with the chemotherapeutic drug on the transplanted tumor of the mouse is higher than that of the treatment scheme group of the single chemotherapy and the single antibody treatment, p is less than 0.05, and the monoclonal antibody combined with the chemotherapeutic drug shows better treatment effect on the transplanted.

The results show that the monoclonal antibody Hetumomab alone can obviously inhibit the growth of the human gastric cancer transplantation tumor, and has obvious pharmacodynamic action of inhibiting the gastric cancer. The monoclonal antibody Hetumomab combined with the chemotherapeutic drug group shows high inhibition rate on transplanted tumors, can obviously inhibit the growth of tumors, has better treatment effect than the single antibody group and the single chemotherapeutic drug group, and shows that the monoclonal antibody combined with the chemotherapeutic drug can effectively inhibit the growth of tumors and reduce the chemotherapy resistance.

TABLE 23 results of monoclonal antibody Hetumomab inhibition of SNU-5 graft tumor growth in human gastric carcinoma in animals

The results of the series of in vivo tumor inhibition experiments of animals prove that the monoclonal antibody Hetumomab has significant inhibition effect (pharmacodynamic effect) on the growth and drug resistance of various human tumors (such as liver cancer, lung cancer and gastric cancer) in vivo, and has important application value for treating the growth, metastasis and drug resistance of various tumors.

Claims

1. A monoclonal antibody or an antigen-binding fragment thereof, wherein the monoclonal antibody is produced by mouse hybridoma cells which are deposited with the China general microbiological culture Collection center (CGMCC) No.12251 at 2016, 3, 16 and 16.

2. A hybridoma cell which is preserved in China general microbiological culture Collection center in 2016, 3, 16 days and with the preservation number of CGMCC No. 12251.

3. A pharmaceutical composition comprising the monoclonal antibody or antigen-binding fragment thereof of claim 1 and a pharmaceutically acceptable carrier.

4. The pharmaceutical composition of claim 3, wherein the monoclonal antibody or antigen-binding fragment thereof is conjugated to a therapeutic moiety selected from the group consisting of a cytotoxin, a radioisotope, or a biologically active protein.

5. Use of the monoclonal antibody or antigen-binding fragment thereof of claim 1 or the pharmaceutical composition of claim 3 or 4 in the manufacture of a medicament for the treatment of a malignant tumor, wherein the malignant tumor is selected from the group consisting of liver cancer, lung cancer, and gastric cancer.

6. Use of the monoclonal antibody or antigen-binding fragment thereof according to claim 1 or the pharmaceutical composition according to claim 3 or 4 for the preparation of a medicament for preventing and/or treating metastasis or recurrence of a malignant tumor, wherein the malignant tumor is selected from the group consisting of liver cancer, lung cancer and gastric cancer.

7. The use of claim 5 or 6, wherein the medicament is for use in combination with a chemotherapeutic agent, an antibody targeting another tumor-specific antigen or radiotherapy.

8. Use of the monoclonal antibody or antigen-binding fragment thereof of claim 1 in the preparation of a kit for detecting the presence of tumor stem cells in a biological sample by a method comprising:

a) contacting the biological sample with the monoclonal antibody or antigen-binding fragment thereof of claim 1;

b) detecting binding of the monoclonal antibody or antigen-binding fragment thereof to a target antigen in the biological sample,

wherein detection of said binding is indicative of the presence of a tumor stem cell in said biological sample, wherein said tumor stem cell is selected from the group consisting of a liver cancer stem cell, a lung cancer stem cell, and a gastric cancer stem cell.

9. The use of claim 8, wherein the biological sample comprises a blood sample, a lymph sample or a component thereof.

10. Use of the monoclonal antibody or antigen-binding fragment thereof of claim 1 in the preparation of a kit for isolating tumor stem cells by a method comprising:

(a) providing a population of cells suspected of comprising tumor stem cells;

(b) identifying a subpopulation of said cells that binds to the monoclonal antibody or antigen-binding fragment thereof of claim 1; and

(c) (ii) isolating the subpopulation(s),

wherein the tumor stem cell is selected from the group consisting of a liver cancer stem cell, a lung cancer stem cell, and a stomach cancer stem cell.

11. Use of the monoclonal antibody or antigen-binding fragment thereof of claim 1 in the preparation of a kit for detecting the presence of malignant tumor in a patient by a method comprising:

a) contacting a biological sample obtained from the patient with the monoclonal antibody or antigen-binding fragment thereof of claim 1;

b) detecting binding of said monoclonal antibody or antigen binding fragment thereof to a target antigen in said biological sample, wherein detection of said binding is indicative of the presence of a malignancy in said patient, wherein said malignancy is selected from the group consisting of liver cancer, lung cancer and stomach cancer.

12. Use of the monoclonal antibody or antigen-binding fragment thereof of claim 1 in the preparation of a kit for prognosing malignant tumor recurrence in a patient, said kit for prognosing malignant tumor recurrence in a patient by a method comprising:

(b) contacting the biological sample comprising circulating cells with the monoclonal antibody or antigen-binding fragment thereof of claim 1; and

(c) identifying the presence of circulating cells that bind to the monoclonal antibody or antigen-binding fragment thereof,

thereby prognosing the recurrence of a malignancy in said patient, wherein said malignancy is selected from the group consisting of liver cancer, lung cancer and gastric cancer.

13. Use of the monoclonal antibody or antigen-binding fragment thereof of claim 1 in the preparation of a kit for prognosing malignant tumor progression in a patient, said kit being for prognosing malignant tumor progression in a patient by a method comprising:

thereby prognosing the progression of a malignancy in said patient, wherein said malignancy is selected from the group consisting of liver cancer, lung cancer and gastric cancer.

14. The use of claim 13, wherein the progression of the malignancy comprises metastasis of the malignancy in the patient.

15. The use of any one of claims 11-14, wherein the biological sample comprises a blood sample, a lymph sample, or a component thereof.