CN107115382A - A kind of method that general flavone is extracted from Guava Leaf - Google Patents
A kind of method that general flavone is extracted from Guava Leaf Download PDFInfo
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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Abstract
The invention discloses a kind of method that general flavone is extracted from Guava Leaf, characterized in that, Guava Leaf is placed in the aqueous solution of surfactant, extracted under the auxiliary of microwave and ultrasound, through separation of solid and liquid after, that is, obtain the described extract solution for including general flavone；In the aqueous solution of described surfactant, the mass percent of surfactant is 1%~3%；The power of microwave is 100~150W；Ultrasonic power is 600~650W.The present invention replaces organic solvent with the aqueous solution, wetting, the solubilization of abundant mating surface activating agent, " body heating " principle of microwave abstracting, and the cavitation effect of ultrasonic wave, conventional organic solvents residue problem is not only solved, while enough greatly shortening extraction time, recovery rate is improved, the production cost of enterprise is reduced, with wide promotion prospect.
Description
Technical field
Field is extracted the present invention relates to plant actives, and in particular to one kind utilizes surfactant collaboration microwave-super
The method that sound wave extracts guava leaf total flavonoid.
Background technology
Guava (Psidium guajava) is Myrtaceae Psidium plant, tropical America is originated in, in China's good fortune
Build, Taiwan, Guangdong, the ground such as Guangxi are distributed or cultivated.Modern pharmacological research shows that Guava Leaf has obvious drop
Blood glucose, antiviral, antibacterial, antidiarrheal, antimalarial isoreactivity.Fitochemical studies show in recent years, extractible in Guava Leaf to have
Effect position belongs to Flavonoids, with good hypoglycemic, antibacterial, effect promoting blood circulation and removing blood stasis, thus is opened with very high
Send out value.
At present, traditional Guava Leaf extracting flavonoids method has " decocting cooking method ", " alcohol dipping extraction method ", " enzymatic isolation method "
" supercritical extract " etc..But, traditional water decoction method extraction efficiency is relatively low, and high-temperature water boils and is easily caused amount of activated material mistake
It is living；Using alcohol extracting method, flavones ingredient recovery rate is higher, but ethanol consumption is big, and the rate of recovery is low, therefore cost is higher, and
Environmental pollution is serious, and industrialization production is inadvisable；Enzymatic Extraction requires that enzyme has high specificity and high activity and enzyme
Method is easily influenceed by many factors such as the species of enzyme, consumption, enzymolysis time, temperature, the more difficult control of production link；It is overcritical
Although extraction extraction efficiency is higher, exists invest excessive in production, this method is limited the problems such as cumbersome
Popularization and application in production.Therefore, a kind of energy consumption of exploitation is low, the time is short, process cleans, technique simple and efficient, and is easy to
The Guava Leaf extracting flavonoids method of industrialized production is particularly important.
Microwave radiation exaraction is temperature effect and Electromagnetic Environmental Effect using microwave to improve the extraction of active substance of plant
Rate.Microwave energy, by directly acting on material molecule, is that whole material is heated simultaneously, i.e., " body heating ", with common heating phase
Than can more reach the effect that energy makes full use of.Microwave radiation exaraction passes through dipole ion rotation and ionic conduction two ways
Inside and outside heat simultaneously, raise cell interior temperature, pressure increase, when pressure exceedes the ability to bear of cell membrane, cell membrane
Rupture, the chromocor compound positioned at cell interior is discharged from cell, is dissolved by the solvent of surrounding, therefore its heat transfer
It is consistent with mass transfer direction, this contributes to the Fast Stripping of flavone compound.Ultrasonic wave is a kind of elastic wave, is had
A series of mechanics, calorifics, ultrasonic effect, cavitation effect, fuel factor and the chemical effect etc. of electromagnetism and chemistry.Ultrasonic wave
Cavitation effect promotes solvent to penetrate into a greater degree in cell, improves the transmission speed of material；And its second order effect such as machine
Tool effect, mechanical oscillation, emulsification, spread, smash and can make cell wall rupture with chemical effect etc. and accelerate flavones chemical combination in plant
The diffusion and release of thing.Surfactant is that a class can also significantly reduce solid-liquid phase table (boundary) face when concentration is very low
The material of power.Surfactant can form molecular aggregate by the suction-operated on surface or interface or in the solution and change
Become the dissolubility of chromocor compound in aqueous, so as to greatly improve the extraction efficiency of flavones.If can replace having with water
Machine solvent does solvent, by surface using the solubilising of surfactant, wetting action, in combination with microwave-ultrasonic advantage in
One, can not only reduce enterprise's production cost, solve the problems, such as organic solvent residual, while Guava Leaf can be greatly improved
The recovery rate of general flavone.
Qu Fenxia etc. (Qu Fenxia, it is old to deposit and Phoebe bourneis group culturation rapid propagating technology research [J] forestry practical techniques, 2010,11：
004.) multistage microwave amplifier bamboo-leaves flavones are have studied, concentration of alcohol, leaf harvest time, solid-liquid ratio and Microwave Extraction time is probed into
Influence of these factors to extracting flavonoids amount, obtaining optimum extraction condition is：Gather blade, solid-liquid ratio 1: 30,60% second June
Determining alcohol, Microwave Extraction time 6min.(Lin Na, the Yin Liguo, Chen Yun's pearl ultrasonic wave extractions hardhead Huang bamboo-leaves flavones work such as Lin Na
Skill optimizes [J] Agriculture of Anhui science, 2011,39 (18)：10816-10817.) carry out carrying for bamboo-leaves flavones using supercritical ultrasonics technology
The optimum extraction condition for taking bamboo-leaves flavones is：Concentration of alcohol 85%, 75 DEG C of Extracting temperature, extraction time 90min, solid-liquid ratio 1:
30, extracting flavonoids rate is 1.178% under the conditions of being somebody's turn to do.Compared with traditional solvent extraction method, ultrasonic extraction and microwave loss mechanisms
Although extracting flavonoids rate can be improved, the raising of recovery rate is not obvious.Ultrasonic wave is used in combination with microwave loss mechanisms, and profit
Acted on surfactant adsorption, extracting flavonoids rate can be effectively improved in the case of without using organic solvent.
The content of the invention
For problem above, a kind of surfactant that provides of the invention cooperates with thin surfacing Guava Leaf
The method of general flavone, it is intended to simplify and extract operation, improves extraction efficiency.
To achieve the above object, the technical solution used in the present invention is：
A kind of method that general flavone is extracted from Guava Leaf, Guava Leaf is placed in the aqueous solution of surfactant,
Extracted under the auxiliary of microwave and ultrasound, through separation of solid and liquid after, that is, obtain the described extract solution for including general flavone；
In the aqueous solution of described surfactant, the mass percent of surfactant is 1%~3%；
The power of microwave is 100~150W；Ultrasonic power is 600~650W.
The present inventor is had found by numerous studies, is done solvent using the aqueous solution of surfactant, is utilized surfactant
Solubilising, wetting action, the cavitation effect of fuel factor and radiation effects and ultrasonic wave in combination with microwave, can be with one
Enterprise's production cost is reduced, organic solvent residual is solved the problems, such as, while the extraction of guava leaf total flavonoid can be greatly shortened
Time, improve extraction efficiency.There is the inventive method step to be simple and convenient to operate, with low cost, extraction efficiency is high, and not deposit
The problem of toxic organic solvents are remained, with very big development potentiality and application value.
The present invention, as Extraction solvent, is made using the aqueous solution dissolved with a small amount of surfactant using surface active agent solubilization
With, the mechanism of galvanomagnetic-effect and ultrasonic wave in combination with microwave, carrying for chromocor compound in Guava Leaf is effectively facilitated
Take efficiency.
Research shows that different classes of surfactant has certain influence on effect of extracting.
Preferably, described surfactant is the mixed of nonionic surface active agent and anionic surfactant
Compound.
The present inventor's research is found, using nonionic surface active agent and the table of the compounding of anionic surfactant
Face activating agent can be from Guava Leaf described in high efficiency extraction general flavone.
Described nonionic surface active agent is Tween-40, Tween-80, Span -60, Span-80；
Further preferably, nonionic surface active agent is at least one of Tween-40, Tween-80.
Described anionic surfactant is lauryl sodium sulfate, dodecyl sodium sulfate.
Further preferably, described anionic surfactant is lauryl sodium sulfate.Lauryl sodium sulfate exists
Solubility with temperature is raised and increased in water, under this specified conditions, and lauryl sodium sulfate coordinates described nonionic table
Face activating agent, extraction effect is more excellent.
The inventors discovered that, the mass ratio of suitable anionic surfactant and nonionic surface active agent is to carrying
Take effect that there is influence, by the scope control under suitable scope, can unexpectedly lift extraction effect.
Still more preferably, described surfactant is the mixture of lauryl sodium sulfate and Tween-80.
Preferably, the mass ratio of anionic surfactant and nonionic surface active agent is 1.5~3.5: 1.
Further preferably, the mass ratio of anionic surfactant and nonionic surface active agent is 2: 1.
Described surfactant is the lauryl sodium sulfate and Tween-80 that mass ratio is 2: 1.
Preferably, in the aqueous solution of surfactant, the mass percent of surfactant is 2%~3%, further
Preferably 2.5~3%.
Preferably, the power of microwave is 120~150W.
Further preferably, the power of microwave is 120W.
Preferably, ultrasonic power is 630~650W.
The present invention can substantially shorten extraction time using described surfactant and microwave, supersonic synergic.As excellent
Choosing, extraction time is 10~20min.
Preferably, Guava Leaf is in advance through drying, pulverization process.
Preferably, Guava Leaf drying to water content is less than or equal into 2%.
Preferably, using fresh Guava Leaf in 50~70 DEG C, freeze-day with constant temperature to described water content.
40 eye mesh screens are crossed after dried Guava Leaf is crushed, screenings is used as extracting raw material.
Preferably, described Guava Leaf and the mass ratio of the aqueous solution of surfactant are 1: 15~40.
The method that a kind of method of preferred extraction guava leaf total flavonoid of the present invention extracts flavones from Guava Leaf, tool
Body comprises the following steps：
The processing of step (1) Guava Leaf
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment to 60 DEG C, freeze-day with constant temperature, when moisture content is low
When 2%, it is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
Step (2) obtains feed liquid after being sufficiently mixed Guava Leaf crushed material and distilled water mass ratio 1: 15~1: 40；
Step (3) is added into the feed liquid of step (2) obtains mixed solution after surfactant, mixing, the surface is lived
Property agent and the aqueous solution mass percent be 2%~3%；
The mixed solution of step (3) is carried out thin surfacing by step (4), and microwave power is 100~150W, is surpassed
Acoustic power is 600~650W, and extraction time is 10~20min；
The product that step (4) is obtained is cooled to room temperature by step (5), depressurizes suction filtration, gained filtrate is containing guava
The solution of leaf flavones.
Still more preferably, in such scheme, the surfactant be at least one of Tween-40, Tween-80 and
The mixture of lauryl sodium sulfate；Wherein, at least one of Tween-40, Tween-80 and the quality of lauryl sodium sulfate
Than for 1: 2；Described surfactant is more preferably lauryl sodium sulfate and Tween-80 (and mass ratio 2: 1).
The microwave power is 120W, and ultrasonic power is 630W, and extraction time is 15min.
Present invention research shows, solvent is used in described surfactant and weight percent；It is matched with again described
Power under microwave and ultrasound penetrate and cavitation effect, lifting solvent can be cooperateed with to the work of " solubilising " of extract component
With, help substantially to shorten extraction time, lifted purpose product recovery rate.
The present invention principle be：Under described extraction system, allow active ingredient dissolution easily.With dissolved with suitable agent
The water of the surfactant of amount replaces organic solvent as Extraction medium, plays the role of to active ingredient " solubilising ".Described
Under the microwave of collaboration and ultrasound, polar molecule can be made to occur resonance friction so as to produce fuel factor, can be with while there is penetrability again
Make heating simultaneously inside described aqueous surfactant solution and material, fuel factor is so not only make use of, simultaneously because cell
It is internal to heat, pressure is generated, cell is easier to rupture, active ingredient can free dissolution.In addition, in described association
With microwave and ultrasound under, " cavitation " caused by medium locally produces the high pressure of moment and destroys cell membrane and cell
Membrane structure, so as to promote cellular plasm by cell membrane and cell membrane, is dissolved in medium, active ingredient is further flowed
Go out；So by triplicity, extraction efficiency can be greatly improved by acting on Guava Leaf extraction process simultaneously, reduce cost.
Advantages of the present invention and beneficial effect：
(1) using the distilled water solution dissolved with a small amount of surfactant as extract solution, it instead of traditional organic solvent.And
Be not belonging to toxic solvent, in the absence of residual the problem of.
(2) Surfactant SDS of the present invention is anion surfactant and non-anion
The mixture of surfactant.Anion surfactant solubility with temperature in water is raised and increased, in this specific bar
Under part, the synergy of anionic and non-anion surface active agent tween -80 can
Significantly improve the recovery rate of Flavonoid substances.
(3) guava leaf total flavonoid is extracted using the method for the invention, extraction time has traditional 2h~5h to shorten to
10~20min, substantially reduces extraction time, greatly reduces energy consumption and cost in production process, improves extraction efficiency.
(4) guava leaf total flavonoid extracted using the method for the invention, due to avoiding prolonged fuel factor,
Reduce flavones and occur thermal decomposition and oxidized degree.
(5) the method for the invention to external environment condition without excessive demand, while required equipment is simple and operating method simply,
With potential application prospect.
Brief description of the drawings
Fig. 1 is that different surfaces activating agent recovery rate under identical conditions is contrasted.
Specific implementation method
In order to be best understood from the present invention, with reference to the example content that the present invention is furture elucidated, but present disclosure
It is not limited solely to following example.
Embodiment 1
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 2: 1 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio,
Mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is 2%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 100W, and ultrasonic power is
600W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 4.96%.
Embodiment 2
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 2: 1 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio,
Mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is
2.5%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 150W, and ultrasonic power is
650W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 5.79%
Embodiment 3
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 2: 1 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio,
Mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is 3%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 120W, and ultrasonic power is
630W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 6.45%.
Embodiment 4
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 3.5 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio:
1, mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is
3%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 120W, and ultrasonic power is
630W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 5.27%.
Embodiment 5
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 1.5 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio:
1, mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is
3%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 120W, and ultrasonic power is
630W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 5.61%.
Embodiment 6
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 3: 1 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio,
Mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is 2%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 100W, and ultrasonic power is
600W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 5.03%.
Embodiment 7
Compare, differ only in embodiment 3, surfactant be dodecyl sodium sulfate and Tween-40 (mass ratio 2:
1).As a result show：General flavone content is 5.03%.
Embodiment 8
Compare, differ only in to embodiment 3, surfactant is dodecyl sodium sulfate and Tween-80 (quality
Than 2: 1).As a result show：General flavone content is 5.12%.
Embodiment 9
Compare, differ only in to embodiment 3, surfactant is dodecyl sodium sulfate and the (quality of Span -60
Than 2: 1).As a result show：General flavone content is 4.43%.
Embodiment 10
Compare, differ only in to embodiment 3, surfactant is dodecyl sodium sulfate and Span-80 (quality
Than 2: 1).As a result show：General flavone content is 4.29%.
Embodiment 11
Compare, differ only in to embodiment 3, surfactant is lauryl sodium sulfate and Tween-40.As a result show
Show：General flavone content is 4.65%.
Embodiment 12
Compare, differ only in to embodiment 3, surfactant is lauryl sodium sulfate and Span -60.As a result show
Show：General flavone content is 4.53%.
Embodiment 13
Compare, differ only in to embodiment 3, surfactant is lauryl sodium sulfate and Span-80.As a result show
Show：General flavone content is 4.57%.
Embodiment 14
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 2: 1 that surfactant sodium dodecyl base sodium sulfonate is added into the feed liquid of step (2) with Tween-80 mass ratio,
Mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is 1%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 100W, and ultrasonic power is
600W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 4.86%.
Comparative example 1
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 2: 1 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio,
Mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is 4%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 100W, and ultrasonic power is
600W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 3.62%.
Comparative example 2
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 2: 1 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio,
Mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is 2%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 50W, and ultrasonic power is
500W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 3.53%.
Comparative example 3
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 2: 1 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio,
Mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is 2%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 200W, and ultrasonic power is
700W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 3.26%.
Comparative example 4
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) Surfactant SDS is added into the feed liquid of step (2), the surfactant accounts for surface
The mass percent of aqueous surfactant solutions is 2%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 100W, and ultrasonic power is
600W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 3.04%.
Comparative example 5
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) surface active agent tween -80 are added into the feed liquid of step (2), the surfactant accounts for blending surface activity
The mass percent of the agent aqueous solution is 2%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 100W, and ultrasonic power is
600W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 2.63%.
Comparative example 6
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) feed liquid of step (2) is subjected to thin surfacing, microwave power is 100W, and ultrasonic power is 600W,
Extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 2.41%.
Comparative example 7
Compare, differ only in embodiment 3, surfactant is Tween-40.As a result show：General flavone content is
2.31%.
Comparative example 8
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 1: 1 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio,
Mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is 2%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 100W, and ultrasonic power is
600W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 2.84%.
Comparative example 9
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 1: 2 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio,
Mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is 2%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 100W, and ultrasonic power is
600W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 2.67%.
Comparative example 10
A kind of method that surfactant cooperates with thin surfacing guava leaf total flavonoid, comprises the following steps：1)
Fresh Guava Leaf is placed in Constant Temp. Oven, temperature adjustment is to 60 DEG C, freeze-day with constant temperature, when moisture content is less than 2%,
It is put into portable pulverizer, after crushing 6 minutes, crosses 40 mesh sieves, obtain Guava Leaf crushed material；
2) precise obtains feed liquid after being sufficiently mixed Guava Leaf crushed material 5g, and distilled water by quality 1: 30；
3) it is 4: 1 that Surfactant SDS is added into the feed liquid of step (2) with Tween-80 mass ratio,
Mixed solution is obtained after mixing, the mass percent that the mixed surfactant accounts for the mixed surfactant aqueous solution is 2%；
4) mixed solution of step (3) is subjected to thin surfacing, microwave power is 100W, and ultrasonic power is
600W, extraction time is 15min；
Using NaNO2-Al(NO3)3- NaOH methods determine the content for the guava leaf total flavonoid that said extracted is obtained, and as a result show
Show：General flavone content is 2.98%.
Above-described embodiment and comparative example are only to demonstrate to illustrate done example, but embodiments of the present invention are not
Limited by above-described embodiment and comparative example, that is made under other any Spirit Essences and principle for not running counter to the present invention changes
Become, modify, substitute, combine, simplify, should be equivalent substitute mode, be included within protection scope of the present invention.
Claims (10)
1. a kind of method that general flavone is extracted from Guava Leaf, it is characterised in that Guava Leaf is placed in surfactant
In the aqueous solution, extracted under the auxiliary of microwave and ultrasound, through separation of solid and liquid after, that is, obtain the described extraction for including general flavone
Liquid；
In the aqueous solution of described surfactant, the mass percent of surfactant is 1%~3%；
The power of microwave is 100~150W；Ultrasonic power is 600~650W.
2. the method for general flavone is extracted from Guava Leaf as claimed in claim 1, it is characterised in that described surface-active
Agent is the mixture of nonionic surface active agent and anionic surfactant.
3. the method for general flavone is extracted from Guava Leaf as claimed in claim 2, it is characterised in that described is non-ionic
Surfactant is Tween-40, Tween-80, Span -60, Span-80；
Described anionic surfactant is lauryl sodium sulfate, dodecyl sodium sulfate.
4. the method for general flavone is extracted from Guava Leaf as claimed in claim 2 or claim 3, it is characterised in that anionic table
The mass ratio of face activating agent and nonionic surface active agent is 1.5~3.5: 1.
5. the method for general flavone is extracted from Guava Leaf as claimed in claim 4, it is characterised in that the power of microwave is
120~150W.
6. the method for general flavone is extracted from Guava Leaf as claimed in claim 5, it is characterised in that ultrasonic power is 630
~650W.
7. the method for general flavone is extracted from Guava Leaf as claimed in claim 6, it is characterised in that live on anionic surface
Property agent and nonionic surface active agent mass ratio be 2: 1.
8. the method for general flavone is extracted from Guava Leaf as claimed in claim 7, it is characterised in that described surface-active
In the aqueous solution of agent, the mass percent of surfactant is 2%~3%.
9. as claimed in claim 1 from Guava Leaf extract general flavone method, it is characterised in that extraction time be 10~
20min。
10. the method for general flavone is extracted from Guava Leaf as claimed in claim 1, it is characterised in that Guava Leaf is advance
Through drying, pulverization process；
Wherein, Guava Leaf drying to water content is less than or equal to 2%；
40 eye mesh screens are crossed after dried Guava Leaf is crushed, screenings is used as extracting raw material；
Described Guava Leaf and the mass ratio of the aqueous solution of surfactant are 1: 15~40.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108553515A (en) * | 2018-05-03 | 2018-09-21 | 中南大学湘雅二医院 | A kind of preparation method of capsule for lowering blood sugar and preventing thirst |
| CN109007834A (en) * | 2018-08-31 | 2018-12-18 | 华南理工大学 | A kind of Fructus psidii guajavae immaturus leaf extract cyclodextrin inclusion compound and the preparation method and application thereof |
| CN112321740A (en) * | 2020-11-12 | 2021-02-05 | 东北农业大学 | Solvent-free high-efficiency extraction method for volatile oil and polysaccharide in guava leaves synchronously by adding lithium ions and combining microwaves |
| CN115974628A (en) * | 2023-02-07 | 2023-04-18 | 广州市果树科学研究所 | Fertilizer for improving quality of guava No. 1 tassel and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103751317A (en) * | 2014-02-20 | 2014-04-30 | 武汉工程大学 | Method for extracting hovenia dulcis thunb total flavones by cooperation of surfactant and microwave-ultrasonic extraction process |
| CN104398542A (en) * | 2014-10-30 | 2015-03-11 | 厦门大学 | Method for extracting and purifying flavones from plants containing flavones |
-
2017
- 2017-06-14 CN CN201710451891.6A patent/CN107115382B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103751317A (en) * | 2014-02-20 | 2014-04-30 | 武汉工程大学 | Method for extracting hovenia dulcis thunb total flavones by cooperation of surfactant and microwave-ultrasonic extraction process |
| CN104398542A (en) * | 2014-10-30 | 2015-03-11 | 厦门大学 | Method for extracting and purifying flavones from plants containing flavones |
Non-Patent Citations (2)
| Title |
|---|
| 徐金瑞等: "番石榴叶黄酮的微波提取及其抗氧化作用研究", 《中国食品学报》 * |
| 杨继生: "《表面活性剂原理与应用》", 31 December 2012, 东南大学出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108553515A (en) * | 2018-05-03 | 2018-09-21 | 中南大学湘雅二医院 | A kind of preparation method of capsule for lowering blood sugar and preventing thirst |
| CN109007834A (en) * | 2018-08-31 | 2018-12-18 | 华南理工大学 | A kind of Fructus psidii guajavae immaturus leaf extract cyclodextrin inclusion compound and the preparation method and application thereof |
| CN112321740A (en) * | 2020-11-12 | 2021-02-05 | 东北农业大学 | Solvent-free high-efficiency extraction method for volatile oil and polysaccharide in guava leaves synchronously by adding lithium ions and combining microwaves |
| CN115974628A (en) * | 2023-02-07 | 2023-04-18 | 广州市果树科学研究所 | Fertilizer for improving quality of guava No. 1 tassel and preparation method thereof |
| CN115974628B (en) * | 2023-02-07 | 2023-10-17 | 广州市果树科学研究所 | Fertilizer for improving quality of guava with red spike number 1 and preparation method thereof |
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