CN107099579A - A kind of seedling stage assay resisting rice sheath blight connects bacterium method - Google Patents
A kind of seedling stage assay resisting rice sheath blight connects bacterium method Download PDFInfo
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- CN107099579A CN107099579A CN201710330029.XA CN201710330029A CN107099579A CN 107099579 A CN107099579 A CN 107099579A CN 201710330029 A CN201710330029 A CN 201710330029A CN 107099579 A CN107099579 A CN 107099579A
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- sheath blight
- seedling stage
- rice sheath
- connects bacterium
- stage assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention belongs to rice sheath blight disease controlling technical field, disclose a kind of seedling stage assay resisting rice sheath blight and connect bacterium method, the mineral water bottle cap (one side leaving certain gaps uncovered by the economic plan) handled well in advance using being placed in rice seedling base portion, place a little absorbent cotton and closure inside (sterilized water moisturizing is instilled on cotton), the mycelium pellet (diameter 0.5mm) cultivated two days is positioned over seedling base portion and is close to plant surface, Coke bottle is put, strict control connects bacterium condition.It is of the invention to be compared with traditional conventional method simply, effectively, more press close to field banded sclerotial blight occurrence condition, bacterium and Resistance Identification are connect suitable for indoor seedling stage banded sclerotial blight；Compared with traditional conventional method, it not only ensure that plant is not easily susceptible to injury, improve determination rates, operating procedure is simple and easily controllable etc., but also it is reliably accurate equally to realize evaluation result.
Description
Technical field
Connect the invention belongs to rice sheath blight disease controlling technical field, more particularly to a kind of seedling stage assay resisting rice sheath blight
Bacterium method.
Background technology
Rice sheath blight disease is caused by Rhizoctonia solani Kuhn (Rhizoctonia solani), is the weight that world paddy rice producing region occurs
Want one of disease.In recent years, with the change of weather, the Rice Production loss that rice sheath blight disease is caused is further serious.In China
The some paddy rice producing regions in south have become the first major disease of influence rice yield, set up and improve resisting rice sheath blight identification
System is the important foundation cultivated resistant variety and carry out resistance mechanism research, and key among these is exactly the paddy rice for science of setting up
Anti- banded sclerotial blight authentication method system, and the authentication method system that different researchers are used at present is not the same.Traditional rice banded sclerotial blight
Sick Resistance Identification method mainly has：Detached-leaf test method and embedding inlay technique (toothpick connects bacterium), such as toothpick connects bacterium leaf sheath position, in reality
Operating process in, it is excessively cumbersome, and easily damage plant.
In summary, the problem of prior art is present be：Traditional rice sheath blight disease authentication method is in actual operation
Cheng Zhong, condition is difficult to control, and disease index statistical difficulty is excessively cumbersome, and wound is caused to plant.
The content of the invention
The problem of existing for prior art, bacterium side is met the invention provides a kind of seedling stage assay resisting rice sheath blight
Method.
The present invention is achieved in that a kind of seedling stage assay resisting rice sheath blight connects bacterium method, the seedling stage assay water
Sheath and culm blight of rice resistance connects bacterium method and comprised the following steps；
Step one, the mineral water bottle cap handled well in advance using being placed in rice seedling base portion, places absorbent cotton and bottle cap
It is internal；
Step 2, is positioned over seedling base portion by the mycelium pellet cultivated two days and is close to plant surface, put Coke bottle, control connects
Bacterium condition.
Further, in the step one：The leaving certain gaps uncovered by the economic plan of mineral water bottle cap one side.
Further, in the step one：Sterilized water moisturizing is instilled on absorbent cotton cotton.
Advantages of the present invention and good effect are：The mineral water bottle cap handled well in advance using being placed in rice seedling base portion
(one side leaving certain gaps uncovered by the economic plan), places a little absorbent cotton and closure inside (sterilized water moisturizing is instilled on cotton), will cultivate the bacterium of two days
Pompon (diameter 0.5mm) is positioned over seedling base portion and is close to plant surface, puts Coke bottle, and strict control connects bacterium condition.Conventional leaf
Sheath connects bacterium and connects bacterium, it is necessary to push rice leaf sheath aside using toothpick method, wheat method or the grain of rice, is easily caused rice seedling damage, right
Experimental result is impacted.This method is compared with conventional method simple, effective, more press close to field banded sclerotial blight occurrence condition, fit
Bacterium and Resistance Identification are connect in indoor seedling stage banded sclerotial blight.Compared with conventional method before, it not only ensure that plant is not easily susceptible to injury,
Improve determination rates, operating procedure is simple and easily controllable etc., but also it is reliably accurate equally to realize evaluation result.
Brief description of the drawings
Fig. 1 is that seedling stage assay resisting rice sheath blight provided in an embodiment of the present invention connects bacterium method flow diagram.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle of the present invention is explained in detail below in conjunction with the accompanying drawings.
As shown in figure 1, seedling stage assay resisting rice sheath blight provided in an embodiment of the present invention, which connects bacterium method, includes following step
Suddenly：
S101：The mineral water bottle cap (one side leaving certain gaps uncovered by the economic plan) handled well in advance using being placed in rice seedling base portion, is placed
A little absorbent cotton and closure inside (sterilized water moisturizing is instilled on cotton)；
S102：The mycelium pellet (diameter 0.5mm) cultivated two days is positioned over seedling base portion and is close to plant surface, cola is put
Bottle, strict control connects bacterium condition.
Indoor sharp eyespot resistance identification is carried out using the method for the present invention, it is main using three test materials：Special green grass or young crops (moderate resistance
Banded sclerotial blight rice varieties), Lei Mengte (height sense banded sclerotial blight rice varieties), special blue or green interference mutant strain.Test result indicates that this connects bacterium
Method is convenient reliable easy, and the main method of laboratory banded sclerotial blight identification is had become at present.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (3)
1. a kind of seedling stage assay resisting rice sheath blight connects bacterium method, it is characterised in that the seedling stage assay rice sheath blight disease resists
Property connects bacterium method and comprised the following steps；
Step one, the mineral water bottle cap handled well in advance using being placed in rice seedling base portion, places absorbent cotton and closure inside；
Step 2, is positioned over seedling base portion by the mycelium pellet cultivated two days and is close to plant surface, puts Coke bottle, and control connects bacterium bar
Part.
2. seedling stage assay resisting rice sheath blight as claimed in claim 1 connects bacterium method, it is characterised in that the step one
In：The leaving certain gaps uncovered by the economic plan of mineral water bottle cap one side.
3. seedling stage assay resisting rice sheath blight as claimed in claim 1 connects bacterium method, it is characterised in that the step one
In：Sterilized water moisturizing is instilled on absorbent cotton cotton.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710330029.XA CN107099579A (en) | 2017-05-11 | 2017-05-11 | A kind of seedling stage assay resisting rice sheath blight connects bacterium method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710330029.XA CN107099579A (en) | 2017-05-11 | 2017-05-11 | A kind of seedling stage assay resisting rice sheath blight connects bacterium method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107099579A true CN107099579A (en) | 2017-08-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710330029.XA Pending CN107099579A (en) | 2017-05-11 | 2017-05-11 | A kind of seedling stage assay resisting rice sheath blight connects bacterium method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110214657A (en) * | 2019-07-23 | 2019-09-10 | 黑龙江省农业科学院绥化分院 | A kind of building method in rice breeding " disease-resistant, anti-fall " two gardens unification identification garden |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101348825A (en) * | 2008-09-08 | 2009-01-21 | 江苏省农业科学院 | Wheat sharp eyespot resistance seedling identification method |
| KR20100041667A (en) * | 2008-10-14 | 2010-04-22 | 문상영 | Method of cultivation of lentinus edodes |
| CN102388695A (en) * | 2011-08-17 | 2012-03-28 | 江苏省农业科学院 | Banded sclerotial blight resistance identification method of wheat seedlings |
| CN102084806B (en) * | 2010-11-16 | 2012-08-08 | 淮阴工学院 | Method for quickly identifying resistance to banded sclerotial blight in rice seedling stage |
-
2017
- 2017-05-11 CN CN201710330029.XA patent/CN107099579A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101348825A (en) * | 2008-09-08 | 2009-01-21 | 江苏省农业科学院 | Wheat sharp eyespot resistance seedling identification method |
| KR20100041667A (en) * | 2008-10-14 | 2010-04-22 | 문상영 | Method of cultivation of lentinus edodes |
| CN102084806B (en) * | 2010-11-16 | 2012-08-08 | 淮阴工学院 | Method for quickly identifying resistance to banded sclerotial blight in rice seedling stage |
| CN102388695A (en) * | 2011-08-17 | 2012-03-28 | 江苏省农业科学院 | Banded sclerotial blight resistance identification method of wheat seedlings |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110214657A (en) * | 2019-07-23 | 2019-09-10 | 黑龙江省农业科学院绥化分院 | A kind of building method in rice breeding " disease-resistant, anti-fall " two gardens unification identification garden |
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Application publication date: 20170829 |
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| RJ01 | Rejection of invention patent application after publication |