CN107099573A - The method that optimization degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide - Google Patents
The method that optimization degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide Download PDFInfo
- Publication number
- CN107099573A CN107099573A CN201710440564.0A CN201710440564A CN107099573A CN 107099573 A CN107099573 A CN 107099573A CN 201710440564 A CN201710440564 A CN 201710440564A CN 107099573 A CN107099573 A CN 107099573A
- Authority
- CN
- China
- Prior art keywords
- duck liver
- freeze
- degreasing
- duck
- dried powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Optimize the method that degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide the present invention relates to a kind of, comprise the following steps：（1）Physiological saline is added after duck liver is shredded, high-speed homogenization adds distilled water, and heat up stir process, is filtrated to get degreasing duck liver albumen；By the volatilization of degreasing duck liver albumen, freeze-drying obtains freeze-dried powder；（2）Freeze-dried powder is added ultrasound is carried out in distilled water；Then trypsase is added, is digested in water-bath, centrifugal filtration takes supernatant；（3）By supernatant hyperfiltration treatment, ultrafiltrate is collected；Ultrafiltrate is freeze-dried, duck liver polypeptide dried powder is obtained.This method is simple to operate, and processing time is short, polypeptide yield is high, and energy consumption is relatively low and zymolyte has the advantages that preferable Scavenging ability.
Description
Technical field
The present invention relates to a kind of method for preparing anti-oxidation peptide, especially a kind of optimization degreasing mode combining ultrasonic auxiliary enzymes
The method that solution duck liver prepares anti-oxidation peptide.
Background technology
China is large agricultural country, and animal products resource is enriched very much, and sizable proportion is occupied in agricultural product total amount.Duck
Liver be duck process accessory substance, its vitamin, content of mineral substances enrich, the adequate proteins of especially natural high-quality it is good
Good source.But most of duck liver is all sold to restaurant in the form of retail and used for small part customer or discarded, many active matters
Matter is not fully used.At present, exploitation natural anti-oxidation health food and natural turn into the section of modem life
Focus is learned, evaluates and natural resources of the screening with strong anti-oxidative activity turns into the new trend of Food Science.At present need badly with
Duck liver is research object, carries out the preparation of duck liver polypeptide and the research of antioxidation activity, for exploitation natural anti-oxidation health food
Scientific basis is provided with the natural added in food.
The content of the invention
The purpose of the present invention be overcome the deficiencies in the prior art there is provided one kind optimization degreasing mode combining ultrasonic it is auxiliary
Help the enzymolysis duck liver method for preparing anti-oxidation peptide, this method is simple to operate, processing time is short, polypeptide yield is high, energy consumption it is relatively low and
Zymolyte has the advantages that preferable Scavenging ability.
The technical scheme provided according to the present invention, the optimization degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares antioxygen
Change the method for peptide, it is characterized in that, comprise the following steps：
(1) high-speed homogenization 1 under the physiological saline of 1~3 times of duck liver weight, 8000~12000rpm is added after duck liver is shredded
~3min, adds the distilled water of 1~3 times of duck liver weight, is warming up to 65~75 DEG C, under 200~400rmp/min at stirring
1~3h is managed, degreasing duck liver albumen is filtrated to get；By degreasing duck liver albumen 1~3h of volatilization, 12 are freeze-dried at -40~-50 DEG C
~24h, obtains freeze-dried powder；
(2) ultrasound is carried out in the distilled water that freeze-dried powder is added to 20~30 times of freeze-dried powder weight；Then add and account for freeze-dried powder
The trypsase of weight 0.5%~1.5%, digests in 45~55 DEG C of water-baths and stirs 90 under 200~400rmp/min
After~150min, go out 10~20min of enzyme in 90~100 DEG C of water-baths；With HCI pH to neutrality, in 8000~
12000rmp, 10~20min is centrifuged under the conditions of 4~8 DEG C, filter to take supernatant；
(3) by supernatant hyperfiltration treatment, ultrafiltrate is collected；Ultrafiltrate is freeze-dried, duck liver polypeptide dried powder is obtained.
In a detailed embodiment, it is filtrated to get after degreasing duck liver albumen, then adds into duck liver in the step (1)
Enter the distilled water of 1~3 times of duck liver weight, be warming up to 65~75 DEG C, 1~3h of stir process, mistake under 200~400rmp/min
Filter, will be filtrated to get degreasing duck liver albumen and is mixed with the degreasing duck liver albumen in step (1).
In a detailed embodiment, ultrasound condition is ultrasonic power in the step (2)：150~250W, when ultrasonic
Between：3~5min, working time 2s, 3~5s of idle hours, supersonic frequency is 20kHz.
In a detailed embodiment, in the supernatant duck liver peptide concentration in 5.3~6.6mg/mL.
In a detailed embodiment, hyperfiltration treatment is the fiber in filtering accuracy 3kD~5kD in the step (3)
Carried out in film.
In a detailed embodiment, ultrafiltrate freeze-drying is -70~-80 by ultrafiltrate in the step (3)
6~12h of pre-freeze at DEG C, then atmospheric pressure be 10~100Pa, temperature be -55~-70 DEG C under the conditions of freeze-drying 24~
72h。
The present invention can remove the fat in duck liver well using atmospheric cooking degreasing, be conducive to follow-up duck liver product
Processing, is a kind of easy to operate duck liver degreasing mode, and is conducive to duck liver proteolysis, saves processing cost, reduces reagent
Pollution to environment；Further combining ultrasonic assistance enzymolysis, simple to operate, processing time is short, polypeptide yield is high, energy consumption it is relatively low and
Zymolyte has the advantages that preferable Scavenging ability.
Brief description of the drawings
Fig. 1 is the graph of a relation of enzymolysis time of the present invention and duck liver peptide concentration.
Fig. 2 is the graph of a relation that enzymolysis time of the present invention and duck liver polypeptide remove ABTS free radical abilities.
Fig. 3 is the graph of a relation of enzymolysis time of the present invention and duck liver protein hydrolysis degree.
Embodiment
With reference to specific embodiment, the invention will be further described.
Method of testing of the present invention is as described below：
First, the measure of degree of hydrolysis：
The measure of degree of hydrolysis refers to pH-stat methods.
In formula：DH-degree of hydrolysis, Nb-NaOH concentration (mol/L), B-NaOH volume (mL), Mp-protein hydrolysate
Total peptide bond number in the quality (g) of matter, htot-substrate protein, pig brain albumen is 7.6mmol/g, and α-duck liver albumen is averaged
Degree of dissociation, (a=[10 (pH-pK)]/[1+10 (pH-pK)] is can be calculated by formula
2nd, the measure of peptide concentration
0.5mL Folin-phenol (reagent A), add 0.5mL sample, 10min are reacted at room temperature, 2mL is added
Folin-phenol (reagent B), 25~30 DEG C of heating 5min of water-bath, 0~4 DEG C of processing 10min of cold bath, are determined at 650nm
Its absorbance, and sample solution is substituted with deionized water do blank control, containing for polypeptide in sample is calculated according to standard curve
Measure (the BSA standard curve ranges of linearity：0~50 μ g/mL).
3rd, the measure of enzymolysis liquid ABTS free radical scavenging activities
(1) preparation (0.01mol/L, pH 7.4) of phosphate sodium buffer solution：Accurately weigh 1.091g Na2HPO4, 0.36g
NaH2PO4·2H2O and 8.78g NaCl, with deionized water dissolving, and are diluted to 1000mL, are kept in dark place, and are used in one week.
(2) preparation of ABTS free-atom aqueous solutions：Take 10mg ABTS and 2.9mg K2S2O810mL sodium phosphate buffers are dissolved in,
Mixing is standby after 25 DEG C of lucifuge reaction 16h, and the 2mL reaction solutions are taken during experiment, 18mL sodium phosphate buffers is added, is made into ABTS
Storing solution, is kept in dark place the same day standby.ABTS storing solutions are diluted with sodium phosphate buffer again, it is surveyed light absorption value at 734nm
0.85 or so, as ABTS working solutions, same day Fresh is used.
(3) assay method：0.1mL sample liquids are taken respectively, 3.9mL ABTS working solutions are added, and are well mixed.Mixed liquor in
10 minutes are stood at room temperature, absorbance (Ai) is determined at wavelength 734nm, replaces sample solution to determine Blank absorbance with distilled water
Spend (A0), absorbance (Aj) is measured instead of ABTS working solutions with sodium phosphate buffer, test parallel do three times.Clearance rate calculates public
Formula is as follows：
Clearance rate (%)=1- (Ai-Aj)/A0 × 100%
Embodiment 1
It is a kind of to optimize the method that degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide, comprise the following steps：
(1) fresh or freezing duck liver is gone into surface and internal membrane, the physiological saline of 1 times of duck liver weight is added after chopping,
High-speed homogenization 3min under 8000rpm, adds the distilled water of 1 times of duck liver weight, is warming up to 65 DEG C, is stirred under 200rmp/min
3h is handled, degreasing duck liver albumen is filtrated to get, then the distilled water of 1 times of duck liver weight is added into duck liver, 65 DEG C are warming up to,
Stir process 3h under 200rmp/min, filtering will be filtrated to get the mixing of degreasing duck liver albumen twice；Degreasing duck liver albumen is placed in
In culture dish, volatilization 1h, 24h is freeze-dried at -40 DEG C, freeze-dried powder is obtained in fume hood；
(2) ultrasound, ultrasonic power are carried out in the distilled water that freeze-dried powder is added to 20 times of freeze-dried powder weight：150W, when ultrasonic
Between：5min, working time 2s, idle hours 3s, supersonic frequency is 20kHz；Then the pancreas egg for accounting for freeze-dried powder weight 0.5% is added
White enzyme, digests in 45 DEG C of water-baths and the enzyme 20min that gone out in 90 DEG C of water-baths is stirred continuously after 90min under 200rmp/min；
With 0.2mol/L HCI pH to neutrality, 20min is centrifuged under the conditions of 8000rmp, 4 DEG C, supernatant is filtered to take；Supernatant
Middle duck liver peptide concentration is in 5.3mg/mL.
(3) supernatant is subjected to hyperfiltration treatment in filtering accuracy 3kD tunica fibrosa, collects ultrafiltrate；By ultrafiltrate-
Pre-freeze 12h at 70 DEG C, then atmospheric pressure be 100Pa, temperature be -55 DEG C under the conditions of be freeze-dried 72h, obtain duck liver polypeptide
Dried powder.
Embodiment 2
It is a kind of to optimize the method that degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide, comprise the following steps：
(1) fresh or freezing duck liver is gone into surface and internal membrane, the physiological saline of 2 times of duck liver weight is added after chopping,
High-speed homogenization 2min under 10000rpm, adds the distilled water of 2 times of duck liver weight, is warming up to 70 DEG C, stirred under 300rmp/min
Processing 2h is mixed, degreasing duck liver albumen is filtrated to get, then the distilled water of 2 times of duck liver weight is added into duck liver, 70 DEG C are warming up to,
Stir process 2h under 300rmp/min, filtering will be filtrated to get the mixing of degreasing duck liver albumen twice；Degreasing duck liver albumen is placed in
In culture dish, volatilization 2h, 18h is freeze-dried at -45 DEG C, freeze-dried powder is obtained in fume hood；
(2) ultrasound, ultrasonic power are carried out in the distilled water that freeze-dried powder is added to 25 times of freeze-dried powder weight：200W, when ultrasonic
Between：4min, working time 2s, idle hours 4s, supersonic frequency is 20kHz；Then the tryptose for accounting for freeze-dried powder weight 1% is added
Enzyme, digests in 50 DEG C of water-baths and the enzyme 15min that gone out in 95 DEG C of water-baths is stirred continuously after 130min under 300rmp/min；With
0.3mol/L HCI pH centrifuges 15min under the conditions of 10000rmp, 6 DEG C, filters to take supernatant to neutrality；Supernatant
Middle duck liver peptide concentration is in 6.6mg/mL.
(3) supernatant is subjected to hyperfiltration treatment in filtering accuracy 4kD tunica fibrosa, collects ultrafiltrate；By ultrafiltrate-
Pre-freeze 9h at 75 DEG C, then atmospheric pressure be 50Pa, temperature be -60 DEG C under the conditions of be freeze-dried 48h, obtain duck liver polypeptide do
Dry powder.
Embodiment 3
It is a kind of to optimize the method that degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide, comprise the following steps：
(1) fresh or freezing duck liver is gone into surface and internal membrane, the physiological saline of 3 times of duck liver weight is added after chopping,
High-speed homogenization 1min under 12000rpm, adds the distilled water of 3 times of duck liver weight, is warming up to 75 DEG C, stirred under 400rmp/min
Processing 1h is mixed, degreasing duck liver albumen is filtrated to get, then the distilled water of 3 times of duck liver weight is added into duck liver, 75 DEG C are warming up to,
Stir process 1h under 400rmp/min, filtering will be filtrated to get the mixing of degreasing duck liver albumen twice；Degreasing duck liver albumen is placed in
In culture dish, volatilization 3h, 12h is freeze-dried at -50 DEG C, freeze-dried powder is obtained in fume hood；
(2) ultrasound, ultrasonic power are carried out in the distilled water that freeze-dried powder is added to 30 times of freeze-dried powder weight：250W, when ultrasonic
Between：3min, working time 2s, idle hours 5s, supersonic frequency is 20kHz；Then the pancreas egg for accounting for freeze-dried powder weight 1.5% is added
White enzyme, digests in 55 DEG C of water-baths and the enzyme that gone out in 100 DEG C of water-baths is stirred continuously after 150min under 400rmp/min
15min；With 0.4mol/L HCI pH to neutrality, 10min is centrifuged under the conditions of 12000rmp, 8 DEG C, supernatant is filtered to take
Liquid；Duck liver peptide concentration is in 6mg/mL in supernatant.
(3) supernatant is subjected to hyperfiltration treatment in filtering accuracy 5kD tunica fibrosa, collects ultrafiltrate；By ultrafiltrate-
Pre-freeze 6h at 80 DEG C, then atmospheric pressure be 10Pa, temperature be -70 DEG C under the conditions of be freeze-dried 24h, obtain duck liver polypeptide do
Dry powder.
Comparative example 1
(1) fresh or freezing duck liver is gone into surface and internal membrane, added after chopping under 2 times of physiological saline, 10000rpm
High-speed homogenization 2min, adds the n-hexane of 2 times of duck liver weight, reflow treatment 5h is filtrated to get degreasing duck liver albumen, by degreasing duck
Orgotein is placed in culture dish, and volatilization 2h, is evacuated to no n-hexane smell, at -45 DEG C in vacuum desiccator in fume hood
Lower freeze-drying 18h, obtains freeze-dried powder；
(2) ultrasound, ultrasonic power are carried out in the distilled water that freeze-dried powder is added to 25 times of freeze-dried powder weight：200W, when ultrasonic
Between：4min, working time 2s, idle hours 4s, supersonic frequency is 20kHz；Then the tryptose for accounting for freeze-dried powder weight 1% is added
Enzyme, digests in 50 DEG C of water-baths and the enzyme 15min that gone out in 95 DEG C of water-baths is stirred continuously after 130min under 300rmp/min；With
0.3mol/L HCI pH centrifuges 15min under the conditions of 10000rmp, 6 DEG C, filters to take supernatant to neutrality；Supernatant
Middle duck liver peptide concentration is in 4.7mg/mL.
(3) supernatant is subjected to hyperfiltration treatment in filtering accuracy 4kD tunica fibrosa, collects ultrafiltrate；By ultrafiltrate-
Pre-freeze 9h at 75 DEG C, then atmospheric pressure be 50Pa, temperature be -60 DEG C under the conditions of be freeze-dried 48h, obtain duck liver polypeptide do
Dry powder.
Comparative example 2
(1) fresh or freezing duck liver is gone into surface and internal membrane, added after chopping under 2 times of physiological saline, 10000rpm
High-speed homogenization 2min, adds 95% ethanol of 2 times of duck liver weight, and reflow treatment 2h is filtrated to get degreasing duck liver albumen, by degreasing
Duck liver albumen is placed in culture dish, and volatilization 2h, is evacuated to no ethanol smell, at -45 DEG C in vacuum desiccator in fume hood
Lower freeze-drying 18h, obtains freeze-dried powder；
(2) ultrasound, ultrasonic power are carried out in the distilled water that freeze-dried powder is added to 25 times of freeze-dried powder weight：200W, when ultrasonic
Between：4min, working time 2s, idle hours 4s, supersonic frequency is 20kHz；Then the tryptose for accounting for freeze-dried powder weight 1% is added
Enzyme, digests in 50 DEG C of water-baths and the enzyme 15min that gone out in 95 DEG C of water-baths is stirred continuously after 130min under 300rmp/min；With
0.3mol/L HCI pH centrifuges 15min under the conditions of 10000rmp, 6 DEG C, filters to take supernatant to neutrality；Supernatant
Middle duck liver peptide concentration is in 5.1mg/mL.
(3) supernatant is subjected to hyperfiltration treatment in filtering accuracy 4kD tunica fibrosa, collects ultrafiltrate；By ultrafiltrate-
Pre-freeze 9h at 75 DEG C, then atmospheric pressure be 50Pa, temperature be -60 DEG C under the conditions of be freeze-dried 48h, obtain duck liver polypeptide do
Dry powder.
From embodiment 1-3 and comparative example 1-2, obtained duck liver is more using atmospheric cooking degreasing hydrolysis result preferably
Peptide concentration highest.
As shown in figure 1, with the extension of enzymolysis time, duck liver peptide concentration increases, and in 130min, peptide concentration reaches
Maximum, up to 6.6mg/mL.
As shown in Fig. 2 with the extension of enzymolysis time, duck liver peptide concentration increases, the ABTS free radicals of duck liver polypeptide
Clearance rate is also in increase, during enzymolysis time 5min, and peptide concentration reaches 1.3mg/mL, and it is 66% to remove ABTS free radicals ability；
By calculating, duck liver polypeptide removes the EC of ABTS free radicals50It is worth for 0.76mg/mL, shows that duck liver polypeptide has preferable
Antioxidation activity.
As shown in figure 3, in enzymolysis time 10-30min, degree of hydrolysis increase is very fast, and enzymolysis time is after 90min, water
Xie Du increases slow down, and illustrate the extension with enzymolysis time, and degree of hydrolysis change is slow.
Claims (6)
1. a kind of optimize the method that degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide, it is characterized in that, including with
Lower step：
（1）High-speed homogenization 1 ~ 3 under the physiological saline of 1 ~ 3 times of duck liver weight, 8000 ~ 12000 rpm is added after duck liver is shredded
Min, adds the distilled water of 1 ~ 3 times of duck liver weight, is warming up to 65 ~ 75 DEG C, the stir process 1 ~ 3 under 200 ~ 400 rmp/min
H, is filtrated to get degreasing duck liver albumen；By degreasing duck liver albumen 1 ~ 3 h of volatilization, 12 ~ 24 h are freeze-dried at -40 ~ -50 DEG C,
Obtain freeze-dried powder；
（2）Ultrasound is carried out in the distilled water that freeze-dried powder is added to 20 ~ 30 times of freeze-dried powder weight；Then add and account for freeze-dried powder weight
0.5% ~ 1.5% trypsase, enzymolysis and 90 ~ 150 min of stirring under 200 ~ 400 rmp/min in 45 ~ 55 DEG C of water-baths
Afterwards, go out the min of enzyme 10 ~ 20 in 90 ~ 100 DEG C of water-baths；With HCI pH to neutrality, in 8000 ~ 12000 rmp, 4 ~ 8 DEG C
Under the conditions of centrifuge 10 ~ 20 min, filter to take supernatant；
（3）By supernatant hyperfiltration treatment, ultrafiltrate is collected；Ultrafiltrate is freeze-dried, duck liver polypeptide dried powder is obtained.
2. the method that optimization degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide as claimed in claim 1, its
It is characterized in：The step（1）In be filtrated to get after degreasing duck liver albumen, then add into duck liver the distillation of 1 ~ 3 times of duck liver weight
Water, is warming up to 65 ~ 75 DEG C, the h of stir process 1 ~ 3 under 200 ~ 400 rmp/min, filtering, will be filtrated to get degreasing duck liver egg
In vain and step（1）In degreasing duck liver albumen mixing.
3. the method that optimization degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide as claimed in claim 1, its
It is characterized in：The step（2）Middle ultrasound condition is ultrasonic power：150 ~ 250 W, ultrasonic time：3 ~ 5 min, working time 2
S, the s of idle hours 3 ~ 5, supersonic frequency is 20 kHz.
4. the method that optimization degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide as claimed in claim 1, its
It is characterized in：Duck liver peptide concentration is in 5.3 ~ 6.6 mg/mL in the supernatant.
5. the method that optimization degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide as claimed in claim 1, its
It is characterized in：The step（3）Middle hyperfiltration treatment is carried out in the kD of the kD of filtering accuracy 3 ~ 5 tunica fibrosa.
6. the method that optimization degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide as claimed in claim 1, its
It is characterized in：The step（3）The freeze-drying of middle ultrafiltrate be by ultrafiltrate at -70 ~ -80 DEG C the h of pre-freeze 6 ~ 12, then big
Atmospheric pressure be 10~100 Pa, temperature be -55~-70 DEG C under the conditions of be freeze-dried 24~72 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710440564.0A CN107099573B (en) | 2017-06-13 | 2017-06-13 | Method for preparing antioxidant peptide by optimizing degreasing mode and combining ultrasonic-assisted enzymolysis of duck liver |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710440564.0A CN107099573B (en) | 2017-06-13 | 2017-06-13 | Method for preparing antioxidant peptide by optimizing degreasing mode and combining ultrasonic-assisted enzymolysis of duck liver |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107099573A true CN107099573A (en) | 2017-08-29 |
| CN107099573B CN107099573B (en) | 2020-12-15 |
Family
ID=59659728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710440564.0A Active CN107099573B (en) | 2017-06-13 | 2017-06-13 | Method for preparing antioxidant peptide by optimizing degreasing mode and combining ultrasonic-assisted enzymolysis of duck liver |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107099573B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110915995A (en) * | 2019-12-03 | 2020-03-27 | 江苏省农业科学院 | Method for preparing pet feed additive from poultry liver protein hydrolysate |
| CN115947787A (en) * | 2022-09-06 | 2023-04-11 | 宁波大学 | Duck liver protein source antioxidant functional peptide and preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1188767A1 (en) * | 2000-08-28 | 2002-03-20 | Kraft Foods Holdings, Inc. | Isolated antioxidant peptides from casein and methods for preparing, isolating and identifying antioxidant peptides |
| CN103204906A (en) * | 2013-01-29 | 2013-07-17 | 浙江海洋学院 | Mussel meat protein antioxidative peptide and preparation method thereof |
| CN104673868A (en) * | 2015-03-18 | 2015-06-03 | 吉林大学 | Method for preparing wild clam meat protein source antioxidant peptide through ultrasound-assisted enzymolysis method |
| CN105820229A (en) * | 2016-04-29 | 2016-08-03 | 贵州大学 | Duck gizzard duck's gizzard antioxidative peptide and application thereof |
| CN106520877A (en) * | 2016-11-23 | 2017-03-22 | 江苏省农业科学院 | Method for preparing pig cerebral protein antioxidative peptide |
| KR20170059501A (en) * | 2015-11-20 | 2017-05-31 | 주식회사 정다운 | Antioxidant peptides derived from duck meat |
-
2017
- 2017-06-13 CN CN201710440564.0A patent/CN107099573B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1188767A1 (en) * | 2000-08-28 | 2002-03-20 | Kraft Foods Holdings, Inc. | Isolated antioxidant peptides from casein and methods for preparing, isolating and identifying antioxidant peptides |
| CN103204906A (en) * | 2013-01-29 | 2013-07-17 | 浙江海洋学院 | Mussel meat protein antioxidative peptide and preparation method thereof |
| CN104673868A (en) * | 2015-03-18 | 2015-06-03 | 吉林大学 | Method for preparing wild clam meat protein source antioxidant peptide through ultrasound-assisted enzymolysis method |
| KR20170059501A (en) * | 2015-11-20 | 2017-05-31 | 주식회사 정다운 | Antioxidant peptides derived from duck meat |
| CN105820229A (en) * | 2016-04-29 | 2016-08-03 | 贵州大学 | Duck gizzard duck's gizzard antioxidative peptide and application thereof |
| CN106520877A (en) * | 2016-11-23 | 2017-03-22 | 江苏省农业科学院 | Method for preparing pig cerebral protein antioxidative peptide |
Non-Patent Citations (8)
| Title |
|---|
| LEE S J等: "Antioxidant activity of a novel synthetic hexa-peptide derived from an enzymatic hydrolysate of duck skin by-products", 《FOOD & CHEMICAL TOXICOLOGY》 * |
| SEUNG-JAE LEE等: "Purification and characterisation of an antioxidative peptide from enzymatic hydrolysates of duck processing by-products", 《FOOD CHEMISTRY》 * |
| 宋春丽: "抗氧化肽的研究进展", 《农产品加工(学刊)》 * |
| 林慧敏等: "鮟鱇鱼肝抗氧化肽的酶法制备及对羟自由基的清除作用", 《中国食品学报》 * |
| 林波等: "不同脱脂方式对牛骨脱脂品质的影响", 《食品科技》 * |
| 王立等: "鸭肝蛋白的超声辅助碱法提取及抗氧化性能研究", 《食品科学》 * |
| 舒沿沿等: "发酵鹅肝肽的分离纯化及其抗氧化特性研究", 《食品工业科技》 * |
| 蒋辉禄: "黄曲霉毒素G1对雏鸭肝脏抗氧化能力的影响", 《黄曲霉毒素G1对雏鸭肝脏抗氧化能力的影响》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110915995A (en) * | 2019-12-03 | 2020-03-27 | 江苏省农业科学院 | Method for preparing pet feed additive from poultry liver protein hydrolysate |
| CN115947787A (en) * | 2022-09-06 | 2023-04-11 | 宁波大学 | Duck liver protein source antioxidant functional peptide and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107099573B (en) | 2020-12-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103980500B (en) | A kind of protein grafting natural polysaccharide as well as preparation method and application thereof | |
| CN103392902B (en) | Method for preparing strong antioxidative peptide by using peanut meal | |
| CN104513843B (en) | A kind of combined preparation process of polysaccharide and protein peptides | |
| CN110734948A (en) | extraction device and process for extracting selenium polypeptide from soybeans | |
| CN107099573A (en) | The method that optimization degreasing mode combining ultrasonic assistance enzymolysis duck liver prepares anti-oxidation peptide | |
| CN107365824A (en) | A kind of preparation method for hydrolyzing Isin glue collagen Gly-His-Lys | |
| CN104313099A (en) | Method for preparing egg albumin peptide with high antioxidant activity | |
| CN109438532B (en) | Method for extracting D-glucosamine | |
| CN108065413A (en) | The method that oyster oligopeptide is prepared using oyster fresh meat | |
| CN104745665A (en) | Collagen peptide with function of promoting bone growth as well as preparation method and application thereof | |
| CN102146427A (en) | Method for preparing swine blood active peptide by microwave-accelerated enzymolysis | |
| CN104593318B (en) | A kind of corn functional peptides additive for cell culture medium | |
| CN102150741B (en) | Method for preparing lactalbumin hydrolysate by utilizing yak milk | |
| CN103952456B (en) | A kind of extracting method of chicken embryo phosvitin phosphoeptide | |
| CN113388657A (en) | Method for preparing food-borne ACE inhibitory peptide by sweep frequency ultrasonic coupling enzymolysis | |
| WO2020224058A1 (en) | Industrialized production method for preparing oyster peptide by means of enzymatic method | |
| CN111635919A (en) | Method for preparing collagen oligopeptide by hydrolyzing animal skin with bacillus subtilis | |
| CN106086139A (en) | A kind of method utilizing fresh-water fishes noggin enzymolysis to prepare fish head polypeptides | |
| CN106244638A (en) | A kind of comprehensive utilization process of biomass circulating fermenting lactic acid | |
| CN110643660B (en) | Method for preparing antioxidant peptide by donkey hide protease hydrolysis under assistance of ultrasound | |
| CN106086132A (en) | A kind of utilize ginkgo nut leather for the method for anti-oxidation peptide | |
| CN104945501A (en) | Iron-chelating collagen peptide of hairtail bone | |
| CN108624645A (en) | A kind of ultrasound wave auxiliary enzyme method prepares the method and its application of tea grounds ace inhibitory peptide | |
| CN105265927A (en) | Preparation method of water-soluble dietary fiber from soybean dregs by microbial fermentation | |
| CN110226666B (en) | Method for reducing lactalbumin allergenicity by using ultrasonic pretreatment and Kluyveromyces marxianus fermentation in combination |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |