CN107096581A - Micro-fluidic paper chip and apply its detecting system - Google Patents
Micro-fluidic paper chip and apply its detecting system Download PDFInfo
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- CN107096581A CN107096581A CN201710441928.7A CN201710441928A CN107096581A CN 107096581 A CN107096581 A CN 107096581A CN 201710441928 A CN201710441928 A CN 201710441928A CN 107096581 A CN107096581 A CN 107096581A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
Abstract
A kind of micro-fluidic paper chip and apply its detecting system, for detecting Sexual Hormone Contents in Serum, micro-fluidic paper chip includes successively in the stacking direction：Filter layer is loaded, for introducing testing liquid, and the cell in retention liquid is filtered；First movement separation layer；Auxiliary electrode layer；Electrode layer；Second movement separation layer；And water accepting layer, for ensureing testing liquid circulation through auxiliary electrode layer and electrode layer and collecting surplus liquid, wherein：Auxiliary electrode layer and electrode layer constitute detection zone layer, including electrochemical sensing circuits, and testing liquid Sex Hormone Levels are detected by electrochemical means；First and second movement separation layers are used for testing liquid and enter the liquid flowing detected after region layer reaction completely between isolation detection zone layer and other layers.
Description
Technical field
The invention belongs to portable medical detection field, more particularly to a kind of micro-fluidic paper chip and its detection system of application
System.
Background technology
Ovary is the reproductive organs of women, and egg cell is generated in ovary, developed, and the ovarian follicle secretion discharge in ovary,
Ovum is fertilized as cytula (embryonated egg) as entered fallopian tubal and running into sperm.Ovary also has endocrine function, and it is synthesized
The sex hormone of secretion controls more than 400 position of the nine big systems such as skeleton, immune, reproduction, nerve, maintains these organs
Youth and vigor.If ovarian function can cause a variety of diseases extremely, such as ovarian failure can cause fertility difficult very
To infertile, the Physiological Psychology health of human body can be also negatively affected.Monitoring of Ovarian Function disease of ovary diagnose, control
Therapeutic effect assessment, standby pregnant guidance and female pathology health etc. have very important significance.By taking infertility as an example, WHO speculates not
Pregnant sterility will be only second to the third-largest disease of tumour and cardiovascular and cerebrovascular diseases as 21 century.Developed country there are about 5%-8%'s
Mr. and Mrs are influenceed by infertility, and the illness rate of some regional infertilities of developing country is up to 30%, and China there are about at present
40000000 Sterility patients, and it is annual with hundreds thousand of speed increases.Infertility is diagnosed, treated and therapeutic effect assessment
It is required for carrying out dynamic monitoring to ovarian function.
The means that are monitored to ovarian function are a lot, it is most common it is optimum be to be commented by the level of internal sex hormone
Estimate ovarian function.Sex hormone is the important endocrine substance of human body, can both adjust ovarian function, is also what ovarian function was detected
Biochemical indicator, can be by detecting that sex hormone quantization level detects the women ovarian function disease related to diagnosis of ovarian.It is conventional
Six, sex hormone i.e. Folliculogenesis hormone (FSH), luteotropic hormone (LH), estradiol (E2), progesterone (P), testosterone (T),
Lactogen (PRL).In six, sex hormone, FSH major functions are the Follicle development and maturations for promoting ovary；LH is mainly rush
Make ovulation, under FSH synergy, form corpus luteum and secrete progestational hormone；Estradiol E2 major functions are to promote endometrium
It is changed into proliferation period and promotes the development of women secondary sex characters.This three joint-detections can meet clinician and scientific research substantially
Demand of the personnel to Monitoring of Ovarian Function.
The sex hormone such as LH, FSH and E2 content in blood of human body is extremely low, it is necessary to higher detection sensitivity, at present detection
Mainly use immunization method, such as immune colloid gold strip, immune biochemical detector method.Wherein colloid gold test strip is based on immune
Chromatography method, simple in construction, easy to operate, price is low, but uses coloration method, is qualitative or half-quantitative detection, sensitivity
It is not high, it is impossible to meet quantitative detection demand.Immune biochemical detector can realize that multi-parameter is detected simultaneously, and detection sensitivity is high,
But detection cycle is long, need sample amount big (using venous blood collection 3-4ml), and immunity analysis instrument is generally large-scale instrument, suitable for hospital
Clinical laboratory or central laboratory, it is difficult to meet family, the personal demand to quick detections such as LH, FSH and E2.
The content of the invention
In view of the problem of existing scheme is present, in order to overcome the shortcomings of above-mentioned prior art, the present invention proposes micro-
Stream controls paper chip and applies its detecting system.
According to an aspect of the present invention there is provided a kind of micro-fluidic paper chip, for detecting Sexual Hormone Contents in Serum, along stacking side
To including successively：Filter layer is loaded, for introducing testing liquid, and the cell in retention liquid is filtered；First movement separation layer；
Auxiliary electrode layer；Electrode layer；Second movement separation layer；And water accepting layer, for ensureing testing liquid circulation through auxiliary
Electrode layer and electrode layer simultaneously collect surplus liquid, wherein：Auxiliary electrode layer and electrode layer constitute detection zone layer, bag
Electrochemical sensing circuits are included, testing liquid Sex Hormone Levels are detected by electrochemical means；First and second movement separation layers
The liquid entered for testing liquid after the reaction completely of detection region layer between isolation detection zone layer and other layers flows.
According to an aspect of the present invention there is provided a kind of detecting system, including：Micro-fluidic paper chip；Electrochemical control mould
Block, for detecting signal to the input of micro-fluidic paper chip；Open-topped box body, houses electrochemical module and the micro-fluidic paper
Chip；And detection means, for closing the open top of the box body, and control the electrochemical control module output detection
Signal, and the optical signal that the detection micro-fluidic paper chip is sent, analyze the content of testing liquid sex hormone.
It can be seen from the above technical proposal that the invention has the advantages that：
Nanometer material and technology and direct immunization electrochemical luminous detection method are combined, establishing only needs single step reaction
Direct immunization electrochemical luminous detection method, simplify operation, improve detection speed；
When electrode layer sets working electrode and differential electrode detection, by the way of time-sharing multiplex, divide in order
Each electrode is not scanned, differential electrode is scanned first, obtained signal is recorded, and 3 working electrodes are then scanned successively,
Difference is carried out to the signal of acquisition, detection signal is obtained, reduces interference, improve detection uniformity and the degree of accuracy；
Mobile separation layer is set, it is to avoid the interference of non-detection layer liquid during detection, testing result is accurate.
Brief description of the drawings
Fig. 1 is the explosive view of micro-fluidic paper chip in one embodiment of the invention；
Fig. 2 is the structural representation of auxiliary electrode layer in Fig. 1；
Fig. 3 is the structural representation of electrode layer in Fig. 1；
The schematic flow sheet that Fig. 4 is prepared for the working electrode in Fig. 1；
Fig. 5 is the structural representation of the first movement separation layer in Fig. 1；
Fig. 6 is the structural representation of the second movement separation layer in Fig. 1；
Fig. 7 is the structural representation of sample-adding filter layer in Fig. 1；
Fig. 8 is the structural representation of water accepting layer in Fig. 1；
Fig. 9 is the structural representation of coating in Fig. 1；
Figure 10 is the structural representation of another embodiment of the present invention detecting system；
Figure 11 is the Cleaning Principle schematic diagram of detecting system in Figure 10.
Embodiment
Certain embodiments of the invention will be done with reference to appended accompanying drawing in rear and more comprehensively describe to property, some of but not complete
The embodiment in portion will be illustrated.In fact, various embodiments of the present invention can be realized in many different forms, and it should not be construed
To be limited to this several illustrated embodiment；Relatively the present invention is caused to meet applicable legal requirement there is provided these embodiments.
In this manual, following various embodiments for being used to describe the principle of the invention are explanation, should not be with any
Mode is construed to the scope of limitation invention.Referring to the drawings described below is used to help comprehensive understanding by claim and its equivalent
The exemplary embodiment of the invention that thing is limited.It is described below to help to understand including a variety of details, but these details should
Think what is be merely exemplary.Therefore, it will be appreciated by those of ordinary skill in the art that not departing from scope and spirit of the present invention
In the case of, embodiment described herein can be made various changes and modifications.In addition, for clarity and brevity,
Eliminate the description of known function and structure.In addition, through accompanying drawing, same reference numerals are used for identity function and operation.
For the object, technical solutions and advantages of the present invention are more clearly understood, below in conjunction with specific embodiment, and reference
Accompanying drawing, the present invention is described in more detail.
One embodiment of the invention provides a kind of micro-fluidic paper chip, and for detecting Sexual Hormone Contents in Serum, Fig. 1 is real for the present invention one
The explosive view of micro-fluidic paper chip 100 in example is applied, as shown in figure 1, micro-fluidic paper chip 100 includes successively in the stacking direction：Cover
Cap rock 10, the sample-adding movement of filter layer 20, first separation layer 30, auxiliary electrode layer 40, the movement separation layer of electrode layer 50, second
60th, water accepting layer 70.Filter layer 20 is wherein loaded, for introducing testing liquid, and the cell in retention liquid is filtered；Water accepting layer 70
For ensureing testing liquid circulation through auxiliary electrode layer and electrode layer and collecting surplus liquid.Auxiliary electrode layer 40 and work
Make electrode layer 50 and constitute detection zone layer, including electrochemical sensing circuits, detect that testing liquid neutrality swashs by electrochemical means
Cellulose content；First movement separation layer 30 and second movement separation layer 20 be used for testing liquid enter detection region layer completely reaction after every
Liquid between exhausted detection zone layer and other layers flows.
The concrete structure of micro-fluidic each layer of paper chip and effect in the embodiment described in detail below.Fig. 2 and Fig. 3 are respectively
Auxiliary electrode layer 40, the structural representation of electrode layer 50 in Fig. 1.
As shown in Fig. 2 auxiliary electrode layer 40 can be using No. 1 filter paper of Whatman as base material, in base material
It is upper first center shunting zone 41 and the N number of first through hole 42 uniformly arranged around the first center shunting zone to be set (N be
N=4 in positive integer more than or equal to 2, the present embodiment), the first center shunting zone 41 is logical by being connected with each first through hole 42
Road 43 is connected, and can be transmitted the testing liquid for being flowed into center shunting zone 41 to each first through hole 42.Center shunting zone 41
On be provided with reference electrode 44 and to electrode 45, be connected to respectively by wire 46 in two output electrodes 47 of auxiliary electrode layer；
Can by silk-screen mode, silk-screen is to electrode 45 (carbon slurry) and reference electrode 44 (Ag/Agcl) on center shunting zone 41, to electricity
Pole 45 and reference electrode 44 and the working electrode formation three-electrode system for the electrode layer 50 introduced.
As shown in figure 3, electrode layer 50 is to use No. 1 filter paper of Whatman as base material, on base material
N number of first hydrophilic area 51 is set, and it, one by one just to setting, cannot not set fixedly anti-with N number of first through hole 42 on one first hydrophilic area
The working electrode 53 of sessile antibody, differential electrode 52 and working electrode are set on the differential electrode 52 of body, other first hydrophilic areas
53 are connected in N number of input electrode 55 of electrode layer by wire 54 respectively.In the first hydrophilic area 51, differential electrode is set
52 and silk-screen carbon is starched first on the first hydrophilic area 51 during working electrode 53, basis of formation electrode prepares difference thereon afterwards
Electrode 52 and working electrode 53.
Fig. 4 is schematic flow sheet prepared by working electrode, and working electrode 53 prepares and uses below scheme, as shown in Figure 4：
1) HAuCl is taken4The aqueous solution, adjusts pH to 7.2, heating is boiled, and sodium citrate is added in the case of quick stirring
Solution, keeps boiling 30 minutes, can obtain nano-Au solution, 4 DEG C of storages are standby.In the case of quick stirring, in Ru
(bpy)3Cl2Add in solution and add nano-Au solution, Ru (bpy)3 2+Ru is combined to form with the electronegative golden nanometer particle in surface
(bpy)3 2+/ nano-Au composite.
2) poly- acetimide (PEI) is added into graphene oxide (GO), under backflow effect, 135 degree are heated 3 hours,
After over cleaning and centrifugation, redox graphene (rGO) is obtained.Thionine molecule (Thi) is acted on by pi-pi accumulation and adsorbed
To the surface of redox graphene, and Ru (bpy)3 2+/ nano-Au composite particle by with the amine on redox graphene
Base reacts and fixes to form compound.
3) after basic electrode surface treated, Ru (bpy) is then added dropwise on basic electrode3 2+/ gold nano/thionine/graphite
Alkene compound formation nano composite membrane.
4) antibody is added dropwise on nano composite membrane, is acted on by the amino of nanogold and protein, antibody is fixed on work
Make on electrode.
5) it is reduction non-specific adsorption, unnecessary avtive spot is finally closed with bovine serum albumin(BSA).
Differential electrode 52 differs only in the sessile antibody of working electrode 53 with working electrode 53, and differential electrode 52 is not
Sessile antibody, the i.e. flow of the preparation of differential electrode 52 and the preparation flow of working electrode 53 are basically identical, only removal step
4)。
Electrode layer differential electrode 52 and working electrode 53 respectively with back work electrode to electrode 45 and reference
Electrode 44 constitutes three-electrode system, during detection, by the way of time-sharing multiplex, scans each differential electrode and work respectively in order
Make electrode.Differential electrode is scanned first, obtained difference optical signal is recorded, 3 working electrodes are then scanned successively, it is right
The optical signal of acquisition carries out difference with difference optical signal, the detection signal of each working electrode of correspondence is obtained respectively, this can subtract
Small interference, improves uniformity.
Dissimilarity hormone antibody can be fixed on different operating electrode 53, such as three working electrodes 53 correspond to fix respectively
The antibody of LH, FSH and E2 sex hormone, the content of detection correspondence sex hormone during each working electrode scanning.
Fig. 5 is the structural representation of the first movement separation layer in Fig. 1, as shown in figure 5, the first movement separation layer 30 equally may be used
Using using No. 1 filter paper of Whatman as base material, including the first fixed part 31 and can be relative to the first fixed part 31
Second of N number of and first through hole 42 one by one just to setting is provided with the first mobile movable part 32, the first fixed part 31
The second center shunting zone 321 being correspondingly arranged with the first center shunting zone 41 is provided with through hole 311, the first movable part 32,
First fixed part 31, which is bonded to, is fixed to auxiliary electrode layer 40, and the first movable part 32 can be relative to the first fixed part 31
Moved between circulating positions and isolated location.When the first movable part 32 relative to the first fixed part 31 positioned at circulating positions
When, in such as Fig. 5 shown in (a), the second center shunting zone 321 is with the first center shunting zone 41 just to setting, it is ensured that testing liquid
Stream is unimpeded, when the first movable part 32 is located at isolated location relative to the first fixed part 31, in such as Fig. 5 shown in (b), the
Two centers shunting zone 321 are shifted to install with the first center shunting zone 41, separate stream.
Fig. 6 is the structural representation of the second movement separation layer in Fig. 1, as shown in fig. 6, the second movement separation layer 60 equally may be used
Using using No. 1 filter paper of Whatman as base material, including the second fixed part 61 and can be relative to the second fixed part 61
It is provided with the second mobile movable part 62, the second movable part 62 and N number of the set is corresponded with the first hydrophilic area 51
Two hydrophilic areas 621, the first fixed part 61, which is bonded to, is fixed to electrode layer 50, and the first movable part 62 can be relative to
One fixed part 61 is moved between circulating positions and isolated location.When the second movable part 62 is relative to the second fixed part 61
During positioned at circulating positions, in such as Fig. 6 shown in (a), the second hydrophilic area 621 and the first hydrophilic area 51 are just to setting, it is ensured that prepare liquid
The stream of body is unimpeded, when the second movable part 62 is located at isolated location relative to the second fixed part 61, (b) institute in Fig. 6
Show, the second hydrophilic area 621 is shifted to install with the first hydrophilic area 51.
Fig. 7 is the structural representation of sample-adding filter layer in Fig. 1, as shown in fig. 7, sample-adding filter layer 20 is public using Whatman
A kind of plasma separation films LF1 (be filter paper) of department is as base material, and this film can effectively dam red in blood
Cell leucocytes, reduce interference of the blood to detection, including the 3rd center shunting zone 21 and positioned at the 3rd center shunting zone 21 weeks
The N number of third through-hole 22 uniformly arranged is enclosed, wherein the 3rd center shunting zone 21 and the first center shunting zone 41 are just to setting, it is N number of
Third through-hole 22 is respectively with N number of second through hole 42 one by one just to setting, and sample-adding filter layer 20 also includes and the 3rd center shunting zone
The adding mouth 23 of 21 UNICOMs, the 3rd center shunting zone 21 is introduced using capillarity by testing liquid.Below scheme can be used
Prepare：
1) filter paper is cut into by A4 sizes using sectional drawing machine；
2) wax spray printing wax spray area is carried out to filter paper using wax spray printer, the 3rd center shunting zone and adding mouth do not spray
Wax.3) heat printed filter paper using accurate numerical control heating instrument, make wax melt to form wax spray area, the shunting of the 3rd center
Area and adding mouth.
3) cut, obtain multiple sample-adding filter layers.
Filter layer 20 is loaded in embodiment can only to shunt at the 3rd center using conventional material as base material
Filter membrane is set in area 21, for filtering the cell in retention liquid.
Fig. 8 is the structural representation of water accepting layer in Fig. 1, as shown in figure 8, water accepting layer 70 is located at micro-fluidic paper chip 100 most
Lower floor, is made of absorbent material, including N number of the 3rd hydrophilic area 71 and water suction with the first hydrophilic area 51 one by one just to setting
Area 72, the suction zones 72 are connected with each 3rd hydrophilic area 71, and suction zones 72 can pass through central pooling zone 73 and each the
Three hydrophilic areas 72 are connected.Main function provides power as prepare liquid collecting tank for liquid flowing, and houses unnecessary to be measured
Liquid.
Fig. 9 is the structural representation of coating in Fig. 1, as shown in figure 9, coating 10 includes N number of and third through-hole 21 1
One fourth hole 11 just to setting, the i.e. position of fourth hole 11 with sample-adding filter layer 20 third through-hole 21, first movement every
The second through hole 311, the first through hole 42 of auxiliary electrode layer 40 and the differential electrode of electrode layer 40 and work electricity of absciss layer 30
Pole is aligned, and effect is to prevent the luminous of working electrode to be blocked.The one side glue material that cover layer 10 can be selected, makees with bonding
With and hydrophobic property, sample-adding filter layer 20 can be bonded to, prevent testing liquid dissipates from overflowing and avoiding sample-adding filter layer by extraneous dirty
Dye.
In the present embodiment, the adding mouth 23 of testing liquid from sample-adding filter membrane 20 flows to the 3rd center shunting zone 21, passes through
By the second center shunting zone 321 of the first movement separation layer 30, the first center shunting zone 41 of auxiliary electrode layer 40 is flow to, then
Flow to 3 working electrodes 53 and 1 differential electrode of electrode layer 50 respectively via first through hole 42 by connecting path 43.
Fixed antibody response on determinand and working electrode 53 in liquid, it is empty when electrode surface can be changed after antigen and antibody binding
Between steric hindrance, suppress electro transfer, reduce electrochemical reaction signal, the reduction of luminous light intensity is caused, by the change for detecting light intensity
The concentration of determinand can be obtained.
Another embodiment of the present invention provides a kind of detecting system 1000, and Figure 10 is another embodiment of the present invention detecting system
Structural representation.As shown in Figure 10, detecting system 1000, including：Micro-fluidic paper chip 100；Electrochemical control module 200, top
The box body 300 and detection means 400. of portion's opening wherein, electrochemical control module 200, for micro-fluidic paper chip input
Detect signal；Open-topped box body 300, houses electrochemical module and the micro-fluidic paper chip；Detection means 400, is used for
The open top of the box body is closed, and controls the electrochemical control module output detection signal, and detects the miniflow
The optical signal that control paper chip is sent, analyzes the content of testing liquid sex hormone.
Specifically, open-topped box body 300 can select shell-type handset port, and detection means 400 can be using intelligent hand
Machine, shell-type handset port can be prepared using 3D printer, it is ensured that interface and mobile phone be closely coupled prevent the interference of ambient light with
And fix electrochemical control module 200 and micro-fluidic paper chip 100 as carrier.The camera of smart mobile phone can be used for detecting micro-
The optical signal that stream control paper chip is sent, and the control of smart mobile phone tool and computing function, for providing control signal, and according to shooting
The optical signal analysis that head is detected goes out measurement result.
Figure 11 is the Cleaning Principle schematic diagram of detecting system in Figure 10, and as shown in figure 11, electrochemical control module 200 includes
Bluetooth communication 201, central controller 202, electric signal output module 203 and electrode interface 204, bluetooth communication 201
Electrochemical control module 201 and the radio communication of smart mobile phone mainly are realized, control signal is obtained from smart mobile phone, and by state
Information back mobile phone；Central controller 202 carries out signal transacting and Behavior- Based control；Electric signal output module 203 is defeated as requested
Go out the electric signal for being used to excite electrochemical substance luminous specified, be connected by electrode interface 204 with micro-fluidic paper chip 100.
Electric signal output module 203 includes multiple working electrode paths (being three in this example), a differential electrode path and a pair
Multiplexing to electrode and reference electrode path.Three working electrode paths different from micro-fluidic paper chip 100 work electricity respectively
Extremely it is connected, different paths is excited in different time sections, it is ensured that the electrode of path to be measured is stimulated luminous, and other paths are not sent out
Light, can thus avoid the cross jamming of different paths.This detecting system point taking pictures and calculation function using smart mobile phone
Realize that electrochemical luminescence is detected.The electric signal output that signal controls electrochemical control module is sent by bluetooth, passes through what is carried
Optical sensor such as camera carries out optical signal detecting, and by calculating the concentration of analysis acquisition determinand.
It should be noted that in accompanying drawing or specification text, the implementation for not illustrating or describing is affiliated technology
Form known to a person of ordinary skill in the art, is not described in detail in field.In addition, the above-mentioned definition to each element and method is simultaneously
Various concrete structures, shape or the mode mentioned in embodiment are not limited only to, those of ordinary skill in the art can carry out letter to it
Singly change or replace.
Particular embodiments described above, has been carried out further in detail to the purpose of the present invention, technical scheme and beneficial effect
Describe in detail bright, it should be understood that the foregoing is only the present invention specific embodiment, be not intended to limit the invention, it is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvements done etc. should be included in the protection of the present invention
Within the scope of.
Claims (10)
1. a kind of micro-fluidic paper chip, for detecting Sexual Hormone Contents in Serum, includes successively in the stacking direction：
Filter layer is loaded, for introducing testing liquid, and the cell in retention liquid is filtered；
First movement separation layer；
Auxiliary electrode layer；
Electrode layer；
Second movement separation layer；And
Water accepting layer, for ensureing testing liquid circulation through auxiliary electrode layer and electrode layer and collecting surplus liquid, wherein：
Auxiliary electrode layer and electrode layer constitute detection zone layer, including electrochemical sensing circuits, are examined by electrochemical means
Survey testing liquid Sex Hormone Levels；
First and second movement separation layers are used for testing liquid into isolation detection zone layer after the reaction completely of detection region layer and its
Liquid flowing between his layer.
2. micro-fluidic paper chip according to claim 1, wherein, the auxiliary electrode layer include the first center shunting zone and
The N number of first through hole uniformly arranged around the first center shunting zone, the first center shunting zone passes through with each first through hole
Connecting path is connected, for the testing liquid for being flowed into center shunting zone to be transmitted to each first through hole, the center shunting
Reference electrode is provided with area and to electrode, is connected to respectively by wire in two input electrodes of auxiliary electrode layer；
The electrode layer includes N number of first hydrophilic area, with N number of first through hole one by one just to setting, on one first hydrophilic area
The working electrode that sessile antibody is set on the differential electrode of not sessile antibody, other first hydrophilic areas, the differential electrode are set
It is connected to respectively by wire in N number of input electrode of electrode layer with working electrode.
3. micro-fluidic paper chip according to claim 2, wherein, the first movement separation layer includes the first fixed part
With the first movable part that can be moved relative to the first fixed part, it is provided with first fixed part N number of with described the
It is provided with the second through hole of one through hole one by one just to setting, first movable part corresponding with first center shunting zone
The the second center shunting zone set, when the first movable part is located at circulating positions relative to the first fixed part, described second
Center shunting zone and first center shunting zone just to setting, when the first movable part relative to the first fixed part be located at every
Off normal when putting, second center shunting zone is shifted to install with first center shunting zone.
4. micro-fluidic paper chip according to claim 2, wherein, the second movement separation layer includes the second fixed part
With the second movable part that can be moved relative to the second fixed part, it is provided with second movable part N number of with described the
One hydrophilic area corresponds the second hydrophilic area set, when the second movable part relative to the second fixed part positioned at circulating positions
When, second hydrophilic area and first hydrophilic area are just to setting, when the second movable part is relative to the second fixed part position
When isolated location, second hydrophilic area is shifted to install with first hydrophilic area.
5. micro-fluidic paper chip according to claim 3, wherein, the sample-adding filter layer include the 3rd center shunting zone and
The N number of third through-hole uniformly arranged around the 3rd center shunting zone, wherein the 3rd center shunting zone and described first
Center shunting zone is just to setting, and N number of third through-hole is respectively with N number of second through hole one by one just to setting, the sample-adding
Filter layer uses filtering film layer for base material, for filtering the cell in retention liquid.
6. micro-fluidic paper chip according to claim 4, wherein, the water accepting layer includes N number of and the first hydrophilic area one by one
Just to the 3rd hydrophilic area of setting and suction zones, the suction zones are connected with each 3rd hydrophilic area.
7. micro-fluidic paper chip according to claim 5, wherein, in addition to coating, the coating includes N number of with the
The fourth hole of three through holes one by one just to setting, the coating have bonding close hydrophobic property, prevent testing liquid dissipate overflow and
Sample-adding filter layer is avoided by outside contamination.
8. a kind of detecting system, wherein, including：
Any described micro-fluidic paper chip in claim 1-7；
Electrochemical control module, for detecting signal to the input of micro-fluidic paper chip；
Open-topped box body, houses electrochemical module and the micro-fluidic paper chip；And
Detection means, for closing the open top of the box body, and controls the electrochemical control module output to detect signal,
And the optical signal that the detection micro-fluidic paper chip is sent, analyze the content of testing liquid sex hormone.
9. detecting system according to claim 8, wherein the detection means includes：
Camera device, for detecting the optical signal that the micro-fluidic paper chip is sent；And
Arithmetic unit, analyzes the content of testing liquid sex hormone.
10. detecting system according to claim 8, wherein, the detection means is mobile phone.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN110170342B (en) * | 2019-03-28 | 2021-07-27 | 东南大学 | Molybdenum disulfide layer-by-layer self-assembly thin film microfluidic chip and preparation method thereof |
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