CN107090450A - The molecular labeling related to millet spike length character and its detection primer and application - Google Patents
The molecular labeling related to millet spike length character and its detection primer and application Download PDFInfo
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Abstract
The invention discloses a kind of molecular labeling related to millet spike length character and its detection primer and application, the molecular labeling is PL 02 016, and it is located at the sequence 38395016bp positions of No. 2 chromosome of millet, and base is C/T.The molecular labeling and its detection primer of the present invention, can predict millet spike length, to realize that the early stage of millet spike length character is identified and screening breeding provides molecule ancillary technique and supported.
Description
Technical field
The present invention relates to Millet Breeding technical field, more particularly to the molecular labeling related to millet spike length character and its inspection
Survey primer and application.
Background technology
China is the original producton location of millet and cultivated area maximum, yield highest are national in the world, and yield accounts for the whole world
The 80% of total amount.Meanwhile, China be also Genetic Resource of Foxtail Millet quantity at most, diversity most abundant country.
Spike length, plant height etc. are to influence the important factor of millet yield, and research is related to control millet spike length Isoquant character
Gene location and its function etc., for instructing millet genetic breeding to have great importance.
SNP (single nucleotide polymorphism, SNP) is by list in genomic level
DNA sequence polymorphism caused by the variation of individual nucleotide base, its quantity in genome is more, widely distributed, genetic stability
It is good.With the development and the reduction of cost of gene sequencing technology, Different Individual same gene or genetic fragment are directly surveyed
Sequence and sequence compare, it may be determined that base is with the presence or absence of variation.Therefore, SNP detections are conducive to Genotyping, it is adaptable to it is quick and
Scale examination is unknown or known SNP and the relation of certain inhereditary feature.
QTL (quantitative trait locus) positioning related on Quantitative Characters of Foxtail Millet at present and SNP marker
Research focuses primarily upon exploitation of SSR marker etc. by way of and utilizing colony self-mating system gene order-checking detection mononucleotide
The development and application research of polymorphism (SNP) molecular labeling not yet has been reported that.Therefore, Quantitative Characters of Foxtail Millet SNP marker is carried out
Exploitation, and set up assist-breeding system, for improving millet yield, save breeding cost significant.
The content of the invention
The present invention provides a kind of molecular labeling related to millet spike length character and its detection primer and application, can predict
Millet spike length, to realize that the early stage of millet spike length character is identified and screening breeding provides molecule ancillary technique and supported.
According to the first aspect of the invention, the present invention provides a kind of SNP marker related to millet spike length character, the SNP
Mark is PL-02-016, and it is located at the sequence 38395016bp positions of No. 2 chromosome of millet, and base is C/T.
Further, such as SEQ ID NO of the sequence where above-mentioned PL-02-016 sites：It is above-mentioned PL-02-016 shown in 3
Point is SEQ ID NO：The 148th bit base from holding 5 ' of sequence shown in 3.
Further, to above-mentioned SNP marker, using composite interval mapping method, using 5% global significance level, QTL inspections
The LOD value of survey is 3.7556, and phenotypic variation explanation rate is 3.35%.
According to the second aspect of the invention, the present invention provides a kind of primer for being used to detect the SNP marker such as first aspect
It is right, including：
Sense primer 7_2F：5’-GTATGCTCCACGCCCTTTA-3’(SEQ ID NO：1) and
Anti-sense primer 7_2R：5’-TTGCGATTACCACTTGATT-3’(SEQ ID NO：2).
According to the third aspect of the invention we, the present invention provides a kind of reagent for being used to detect the SNP marker such as first aspect
Box, including such as primer pair of second aspect, and the optional agent formulations expanded for PCR, these agent formulations can be wrapped
Include PCR buffer solutions, dNTPs, archaeal dna polymerase etc..
According to the fourth aspect of the invention, the present invention provides a kind of method for detecting the SNP marker such as first aspect, uses
Such as the primer pair of second aspect, performing PCR amplification is entered to millet genomic DNA to be detected, and divide by the way that amplified production is sequenced
Analyse the base situation in PL-02-016 sites.
According to the fifth aspect of the invention, the primer pair that the present invention is provided such as second aspect is being detected such as first aspect
Application in SNP marker.
According to the sixth aspect of the invention, the present invention provides a kind of millet spike length Forecasting Methodology, using such as second aspect
Primer pair, performing PCR amplification is entered to millet genomic DNA, and analyze by the way that amplified production is sequenced the base in PL-02-016 sites
Situation, and then predict millet spike length.
According to the seventh aspect of the invention, the SNP marker that the present invention is provided such as first aspect is predicted or paddy in millet spike length
Application in the early stage identification of sub- spike length character or millet marker assisted selection.
According to the eighth aspect of the invention, the primer pair that the present invention is provided such as second aspect is predicted or paddy in millet spike length
Application in the early stage identification of sub- spike length character or millet marker assisted selection.
The beneficial effects of the invention are as follows：The SNP marker related to millet spike length character that the present invention is provided can be used for paddy
The molecular mark of sub- spike length character, millet spike length can be predicted by the SNP marker, to realize millet spike length character
Early stage identification and screening breeding molecule ancillary technique is provided and supported, hereditary and selection and improvement process for accelerating millet variety
With important theory and practice directive significance.The specific primer of the present invention can carry out good parting, inspection to millet spike length
Survey the difference of SNP expression.
Brief description of the drawings
Fig. 1 is millet Parent genome dna electrophoresis glue figure in the embodiment of the present invention, and wherein M swimming lanes are represented
DL2000marker；1 swimming lane represents Zhang Gusan genomic DNAs；2 swimming lanes represent A2 genomic DNAs.
Fig. 2 be the embodiment of the present invention in millet Parent Genomic PCR products running gel figure, wherein A7-2 represent with
The PCR primer that A2DNA is template, 7-2 is primer, 37-2 is represented by template of Zhang Gusan DNA, 7-2 produces for the PCR of primer
Thing, M represents DNA marker clip size, sequentially consist of 100bp, 250bp, 500bp, 750bp, 1000bp,
2000bp。
Fig. 3 is the comparison result figure of the maternal sequence with where male parent SNP marker PL-02-016 in the embodiment of the present invention,
SNP marker PL-02-016 is located at No. 2 chromosome, 38395016bp positions, and maternal base is C, and male parent base is T.
Embodiment
The present invention is described in further detail below by embodiment combination accompanying drawing.
The embodiment of the present invention provides molecular labeling, primer and its application related to millet spike length character.The present invention is implemented
The millet spike length close linkage SNP marker PL-02-016 of example, positioned at sequence 38395016bp of No. 2 chromosome of millet
(reference gene group --- the base number on Yugu genomes) is put, base is C/T.
What deserves to be explained is, bin refers in Bin mappings (Bin map) in the present invention, utilizes weight whole in mapping population
Group site information, obtains the least unit (Recombination bin) for constituting recombination event.Mark is used as by the use of obtained bin
Remember and built for follow-up linkage map and QTL positioning.
In embodiments of the present invention, genetic map (genetic map or linkage map) refers to weight between genetic marker
The linear array figure of relative position between the mark built based on group rate.Passed through based on high flux weight sequencing technologies to mapping group
Body parent and progeny population are sequenced, using a number of continuous SNP as the foundation for judging filial generation recombination site, obtain every
The full-length genome physics restructuring collection of illustrative plates of individual filial generation.Using recombination site information whole in mapping population, obtain and constitute restructuring thing
Least unit (Recombination bin) and the Bin figure (Bin map) of part, and utilize after obtained bin is used for as mark
Continuous linkage map is built and QTL positioning.
The embodiment of the present invention provide millet male parent " No. Zhang Gusan " and maternal " A2 " material (with millet sterile line 1066A and
Millet photo-thermo-sensitive genetic male sterile line 821 hybridize after, through being formed more for seed selection), the two hybridization F1 generation material and the F2 in the generation of selfing 13 groups
441 parts of materials of body.Each part material is planted in body in Zhangjiakou Area, Hebei Province, and records and arrange the trait datas such as spike length.By each part material
Carry out degeneracy genome to resurvey after sequence, compare the splicing of reference gene group and complete, obtain SNP marker.By analyzing spike length character number
According to, the associated SNP marker of acquisition, and the SNP marker is verified by cloning and sequencing.
Describe technical scheme and technique effect in detail by the following examples, it will be appreciated that embodiment is only
Exemplary, for the result for illustrating the feasibility of the present invention and obtaining, it is impossible to be interpreted as the limit to the scope of the present invention
System.
Embodiment 1：High-flux sequence and SNP marker information analysis
The F1 generation material that the present embodiment is obtained using millet male parent " confused flour beetle 3 " and maternal " A2 " material, the two hybridization
And 441 parts of materials of the F2 colonies obtained after the generation of F1 generation selfing 13, i.e., RIL (Recombinant inbred lines,
RILs).Each part material is planted in body in Zhangjiakou Area, Hebei Province, and records and arrange the trait datas such as spike length.Each part material is subjected to letter
And genome is resurveyed after sequence, compare the splicing of reference gene group and complete, obtain SNP marker.By analyzing spike length trait data, obtain
Associated SNP marker, and the SNP marker is verified by cloning and sequencing.
The present embodiment utilizes RADseq methods, and extracting each individual of sample, (wherein 441 RILs, 2 parents, 1 F1 are individual
Body) genomic DNA and carry out DNA quality testings, carry out digestion to qualified DNA, electrophoresis reclaims DNA fragmentation, and plus connecing
Head carries out cluster (cluster) and prepared, finally upper machine sequencing.
The present embodiment is comprised the following steps that：
(1) the fresh blades of 1.0g are weighed, shreds and is put into mortar, with 1.5 × CTAB of 3mL are added after liquid nitrogen grinding, are ground to form
Homogenate is transferred in 15mL centrifuge tube, is then rinsed toward 1.5 × CTAB of addition 1mL in mortar, then be transferred in centrifuge tube.Mix
After 65 DEG C of water-bath 30min, during which slowly shake up frequently.
Wherein 1.5 × CTAB is formulated (1L) such as table 1 below：
Table 1
| Composition | Consumption |
| CTAB | 15g |
| 1mol/L Tris.Cl (pH is 8.0) | 75mL |
| 0.5mol/L EDTA | 30mL |
| NaCl | 61.4g |
Plus deionized water is settled to 1L, the preceding mercaptoethanol for adding final concentration of 0.2% (2ml) is used.
(2) room temperature is cooled to, isometric chloroform/isoamyl alcohol (24 is added：1), gently mix, be changed into dark green to subnatant
Color.
(3) 4200rpm centrifuges 10min, and upper strata aqueous phase moves on to new 15mL centrifuge tubes, plus 2 times of volume precoolings is anhydrous
Ethanol, mixes static 5min；30min precipitations DNA is placed in -20 DEG C.
(4) 4200rpm centrifuges 10min, discards supernatant, adds the ethanol of 1mL 75% washing precipitation 1 time, is inverted centrifuge tube and does
Dry DNA, adds 50 μ L TE dissolving DNAs.
(5) DNA concentration is detected, and is adjusted to water 20ng/ μ L.
(6) digestion is carried out using PstI enzymes, interrupts genomic DNA, reaction system such as table 2 below：
Table 2
(7) reaction, reaction system such as table 3 below are attached：
Table 3
(8) each sample respectively takes 1 μ L reaction products, adds in a new centrifuge tube, the μ L of cumulative volume 12.Every 12 samples
One group.
(9) 300-700bp size fragments are cut after reclaiming gel electrophoresis 1h, EB dyeing with 3%.Carried out with QIAquickKit
Glue purification is reclaimed, and recovery product is dissolved in 30 μ L EB solution.
(10) performing PCR reaction, PCR reaction systems such as table 4 below are entered：
Table 4
PCR response procedures are as follows：94 DEG C of pre-degenerations 5 minutes；94 DEG C are denatured 30 seconds, and 60 DEG C are annealed 30 seconds, 72 DEG C of extensions 40
Second, run 10 circulations；Last 72 DEG C extend 3 minutes.
(11) storehouse is built in magnetic beads for purifying, completion.Specifically purification process is：First, 1.2 times of volume magnetic beads are added after PCR, are stood
10min.Then, it is placed on magnetic frame and adsorbs, removes supernatant.Then, the ethanol of 500 μ L 70% is added to wash twice.On xeothermic instrument
After being evaporated, 15 μ L EB solution dissolving 5min is added.Finally, adsorbed on magnetic frame, transfer supernatant is in 1.5ml centrifuge tubes.
(12) detection reaches upper machine sequencing after machine standard.
As a result：444 samples (wherein 441 RILs, 2 parents, 1 F1 individual) are carried out with digestion and builds storehouse sequencing, is obtained
To 75.99Gb initial data, average each individual 171.54Mb.Sequencing sequence is compared to reference gene group (i.e. Yugu genes
Group, can obtain the sequence of Yugu genomes from following Internet address hffp：https://www.ncbi.nlm.nih.gov/genome/
Term=Setaria+italica+ (foxtail+millet)) on, average comparison rate 86.326%, average coverage rate
8.226%, average sequencing depth 3.655X.
By sequencing and information analysis, there are 33771 SNP markers of polymorphism between acquisition parent.According to window sliding
Method, it is a window to choose several SNP, and genotype and each individual that a SNP determines each window are slided every time
Exchange site.Ultimately produce bin genotype.According to bin genotype datas, with MSTMap software building genetic maps, 2022
Individual bin is navigated on 9 chromosomes, then genetic map data is imported into MapChart softwares, integrates a genome heredity
Linkage map.
Using constructed millet dense genetic map, (CIM) is analyzed using composite interval mapping, to millet spike length
Shape phenotype carries out qtl analysis.QTL detections, according to 500 permutation tests results, determine spike length using 5% global significance level
The critical LOD value of trait phenotypes data qtl analysis, analysis obtains related to spike length prediction bin intervals and SNP site information.
As a result show, the molecular labeling PL-02-016 related to millet spike length character, positioned at genetic linkage mapses the 2nd
The centimorgan of chromosome 237.81 (cM), the 201st bin to the 202nd bin (chr2_bin201-chr2_bin202), using compound
Interval Mapping, using 5% global significance level, the LOD value of QTL detections is 3.7556, and phenotypic variation explanation rate is
3.35%, additive effect value (Additiveeffect, A) is -0.8594.
Embodiment 2：SNP marker is verified
According to the bin of prediction intervals and SNP site, reference gene group is compared, relevant range gene order is obtained, chosen
300bp or so before and after SNP site, designs and develops SNP marker primer, enters performing PCR as template using male parent and female parent material DNA and expands
Increase.Selection primer amplification is normal, PCR primer meets the amplified production for predicting size, and recovery product is simultaneously sequenced, and selects male parent
There is the labeled primer of SNP site difference with female parent material amplification gene sequence.
Multiple SNP sites are included in the bin marks of prediction, these sites are screened according to PCR results.
First, the sequencing result after being reclaimed according to PCR primer, selection male parent and female parent material amplification gene sequence have SNP
The mark of Site discrepancy.Comprise the following steps that：
(1) according to step (1) to (4) in embodiment 1, Parent genomic DNA is extracted respectively with CTAB methods.
(2) genomic DNA, millet Parent genome dna electrophoresis glue figure such as Fig. 1 are detected with 0.8% Ago-Gel
It is shown.
(3) by obtained Parent genomic DNA be stored in -20 DEG C it is standby.
(4) 7_2F is utilized using the male parent of extraction and maternal genomic DNA as template respectively：5’-
GTATGCTCCACGCCCTTTA-3’(SEQ ID NO：And 7_2R 1)：5’-TTGCGATTACCACTTGATT-3’(SEQ ID
NO：2) amplimer enters performing PCR amplification.
PCR reaction systems such as table 5 below：
Table 5
| Reagent | Consumption |
| Sterilized water | 20.2μL |
| 10 × buffer solution (contains Mg2+) | 2.5μL |
| dNTPs(25mM) | 0.15μL |
| Taq enzyme (5U/ μ l) | 0.15μL |
| Forward primer (10 μm of ol/L) | 0.5μL |
| Reverse primer (10 μm of ol/L) | 0.5μL |
| Template | 1.0μL |
| Cumulative volume | 25μL |
PCR response procedures are as follows：94 DEG C of pre-degenerations 5 minutes；94 DEG C are denatured 30 seconds, and 60 DEG C are annealed 30 seconds, 72 DEG C of extensions 40
Second, run 35 circulations；Last 72 DEG C extend 3 minutes.Pcr amplification product is purified after preservation at 4 DEG C.Each PCR amplifications production
Thing takes part to carry out 1% agarose gel electrophoresis detection, as a result as shown in Figure 2.
By the way that pcr amplification product is sequenced, the sequencing for the maternal amplified production that primer 7_2F and 7_2R amplification are obtained
SEQ ID NO in sequence such as sequence table：Shown in 3, PL-02-016 sites are SEQ ID NO：Sequence shown in 3 is the 148th from holding 5 '
Bit base.The sequencing sequence comparison result of maternal amplified production and male parent amplified production is as shown in figure 3, the base table of its acceptance of the bid ash
Show the base in PL-02-016 sites.
Then, according to sample spike length data screening：Male parent spike length is 26cm, and maternal spike length is 19cm, in 441 parts of samples,
In minimum value 17cm, maximum 47cm, spike length length most short 50 samples, the SNP is more than 70%, spike length with maternal identical
In length most long 50 samples, 80% is more than with male parent SNP identicals sample number.
Above content is to combine specific embodiment further description made for the present invention, it is impossible to assert this hair
Bright specific implementation is confined to these explanations.For general technical staff of the technical field of the invention, do not taking off
On the premise of from present inventive concept, some simple deduction or replace can also be made, the protection of the present invention should be all considered as belonging to
Scope.
SEQUENCE LISTING
<110>Academy of Agriculture, Zhangjiakou City
<120>The molecular labeling related to millet spike length character and its detection primer and application
<130> 16I23502
<160> 3
<170> PatentIn version 3.3
<210> 1
<211> 19
<212> DNA
<213>Sense primer for detecting PL-02-016
<400> 1
gtatgctcca cgcccttta 19
<210> 2
<211> 19
<212> DNA
<213>Anti-sense primer for detecting PL-02-016
<400> 2
ttgcgattac cacttgatt 19
<210> 3
<211> 281
<212> DNA
<213>Sequence where PL-02-016 sites
<220>
<221>SNP site
<222> (148)..(148)
<223>N is C or T
<400> 3
ccctttagca gagcgattgc acgaaacaaa agcctcgtat ccttcagcca ttatgacatc 60
tgcagacagg tcctgcctcg acaatttggt ctcctacata caacgcagaa ggcattgata 120
cagtttggca aatgaatcac aaaccaancc ccatttcacc cagttttact atttgcattt 180
gactgaacaa acgatccaat aacatcccac cacagaaccg acaatttgat cagcattaaa 240
cgagatacac taattttttt ctgaaatcaa gtggtaatcg c 281
Claims (10)
1. the SNP marker related to millet spike length character, it is characterised in that the SNP marker is PL-02-016, it is located at paddy
The sequence 38395016bp positions of sub No. 2 chromosome, base is C/T.
2. the SNP marker related to millet spike length character according to claim 1, it is characterised in that the PL-02-
Sequence such as SEQ ID NO where 016 site：Shown in 3, the PL-02-016 sites are SEQ ID NO：Sequence shown in 3 is from 5 '
The 148th bit base is held.
3. the SNP marker related to millet spike length character according to claim 1 or 2, it is characterised in that to the SNP
Mark, using composite interval mapping method, using 5% global significance level, the LOD value of QTL detections is 3.7556, phenotypic variation
Explanation rate is 3.35%.
4. a kind of primer pair for being used to detect the SNP marker as described in claim any one of 1-3, it is characterised in that including：
Sense primer 7_2F：5’-GTATGCTCCACGCCCTTTA-3’ （SEQ ID NO：1）With
Anti-sense primer 7_2R：5’-TTGCGATTACCACTTGATT-3’ （SEQ ID NO：2）.
5. a kind of kit for being used to detect the SNP marker as described in claim any one of 1-3, it is characterised in that including such as
Primer pair described in claim 4, and the optional agent formulations expanded for PCR.
6. a kind of method for detecting the SNP marker as described in claim any one of 1-3, it is characterised in that will using such as right
The primer pair described in 4 is sought, performing PCR amplification is entered to millet genomic DNA to be detected, and analyze by the way that amplified production is sequenced
The base situation in PL-02-016 sites.
7. application of the primer pair as claimed in claim 4 in the SNP marker as described in claim any one of 1-3 is detected.
8. a kind of millet spike length Forecasting Methodology, it is characterised in that primer pair as claimed in claim 4 is used, to millet gene
Group DNA enters performing PCR amplification, and analyzes by the way that amplified production is sequenced the base situation in PL-02-016 sites, and then predicts millet
Spike length.
9. the SNP marker as described in claim any one of 1-3 is reflected early stage the prediction of millet spike length or millet spike length character
Application in fixed or millet marker assisted selection.
10. primer pair as claimed in claim 4 identification or millet early stage the prediction of millet spike length or millet spike length character
Application in marker assisted selection.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707683A (en) * | 2018-04-16 | 2018-10-26 | 张家口市农业科学院 | With the relevant SNP marker of millet spike length character and its detection primer and application |
| CN108715900A (en) * | 2018-04-16 | 2018-10-30 | 张家口市农业科学院 | With the relevant SNP marker of millet fringe principal characteristic shape and its detection primer and application |
| CN109136399A (en) * | 2018-04-16 | 2019-01-04 | 张家口市农业科学院(河北省高寒作物研究所) | One kind SNP marker relevant to millet fringe principal characteristic shape and its detection primer and application |
| CN114317810A (en) * | 2022-02-25 | 2022-04-12 | 山西农业大学 | SNP (Single nucleotide polymorphism) site and KASP (Kaempferi protein) molecular marker primer group for identifying millet folic acid traits and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154281A (en) * | 2011-03-24 | 2011-08-17 | 深圳华大基因科技有限公司 | Molecular marker SIsv0010 closely linked with heading-date gene of millet |
-
2017
- 2017-03-24 CN CN201710183552.4A patent/CN107090450B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154281A (en) * | 2011-03-24 | 2011-08-17 | 深圳华大基因科技有限公司 | Molecular marker SIsv0010 closely linked with heading-date gene of millet |
Non-Patent Citations (2)
| Title |
|---|
| GUANQING JIA等: "A haplotype map of genomic variations and genome-wide association studies of agronomic traits in foxtail millet (Setaria italica)", 《NATURE GENETICS》 * |
| 冯小磊等: "谷子研究进展", 《AGRICULTURAL SCIENCE & TECHNOLOGY》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108707683A (en) * | 2018-04-16 | 2018-10-26 | 张家口市农业科学院 | With the relevant SNP marker of millet spike length character and its detection primer and application |
| CN108715900A (en) * | 2018-04-16 | 2018-10-30 | 张家口市农业科学院 | With the relevant SNP marker of millet fringe principal characteristic shape and its detection primer and application |
| CN109136399A (en) * | 2018-04-16 | 2019-01-04 | 张家口市农业科学院(河北省高寒作物研究所) | One kind SNP marker relevant to millet fringe principal characteristic shape and its detection primer and application |
| CN108707683B (en) * | 2018-04-16 | 2021-12-21 | 张家口市农业科学院 | SNP (Single nucleotide polymorphism) marker related to grain ear length character as well as detection primer and application thereof |
| CN108715900B (en) * | 2018-04-16 | 2021-12-21 | 张家口市农业科学院 | SNP (Single nucleotide polymorphism) marker related to weight of millet ears as well as detection primer and application thereof |
| CN114317810A (en) * | 2022-02-25 | 2022-04-12 | 山西农业大学 | SNP (Single nucleotide polymorphism) site and KASP (Kaempferi protein) molecular marker primer group for identifying millet folic acid traits and application thereof |
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