CN107064527A - A kind of seralbumin detection kit - Google Patents
A kind of seralbumin detection kit Download PDFInfo
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- CN107064527A CN107064527A CN201710237555.1A CN201710237555A CN107064527A CN 107064527 A CN107064527 A CN 107064527A CN 201710237555 A CN201710237555 A CN 201710237555A CN 107064527 A CN107064527 A CN 107064527A
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- albumin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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Abstract
The invention discloses a kind of seralbumin detection kit, this kit is detected the seralbumin in blood using Bromocresol green, belongs to clinical vitro detection reagent technique field.Kit of the present invention only includes reagent R.By adding sodium chloride and TritonX 100 in reagent R, the stability of kit is improved, preferably, the degree of accuracy of reagent is also preferable, is conducive to further promoting the use of in the market for the range of linearity.
Description
Technical field
The present invention relates to clinical vitro detection reagent technique field, more particularly to a kind of seralbumin detection kit.
Background technology
Seralbumin is synthesized by liver parenchymal cell, and its half-life period is about 15-19 days in blood plasma, is contained in blood plasma
Amount is most, molecule is minimum, a kind of protein that solubility is big, function is more, accounts for the 40%-60% of Total plasma protein.Albumin
Molecular structure was illustrated in 1975, the single chain protein matter that it is made up of 585 amino acid, and its molecular weight is about 67kD, into
Ripe albumin is one heart-shaped molecule, is made up of the similar α-helixstructure domain of 3 structures.
The content of protein can influence the synthesis of albumin in food, in addition, its synthesis is also adjusted by plasma levels,
Albumin is not almost stored in liver cell, and micro albumin is all contained in all extracellular fluids.Body fluid pH7.4's
In environment, albumin is anion, and per molecule is with more than 200 negative electrical charges.Albumin is the main carriers in blood plasma, can be with
The small organic molecule and inorganic ions of internal many poorly water-solubles reversibly combine to form the compound of ease of solubility, as these
Types of transportation of the material in blood circulation, including bile salt, bilirubin, long chain fatty acids, steroid hormone, prostatitis
Parathyrine, metal ion and some drugses.Albumin is a kind of with stickiness, collagenous material, in heavy metal in human body ion
In the presence of, it can be combined automatically with heavy metal ion, so as to be excreted by excretory system, play a part of removing toxic substances.Therefore, eat
The abundant food of albumin content, can avoid the absorption of heavy metal ion and be poisoned.
Albumin content rise mainly has two reasons, and one is due to pachyemia so that albumin relative amount is raised,
It is common in serious dehydration and shock, serious burn, massive haemorrhage, kidney trouble etc.；Two be the shadow by protein intake in diet
Ring, thus to a certain extent albumin can as nutrition condition index.Albumin content reduction be common in it is malnutritive,
Acute and chronic hepatitis, Chronic gastrointestinal diseases, diabetes, high fever, wound etc..
The present invention provides a kind of Bromocresol green and detects sero-abluminous kit, optimizes its reaction system, makes its heavy
Renaturation is good, stability is good, can carry out the full-automation detection of batch sample.
The content of the invention
It is an object of the invention to provide a kind of stability it is good be used for detect sero-abluminous kit, this kit is adopted
Use Bromocresol green.This kit is compared with similar kit, and stability is better than similar detection kit with linear dependence, has
Beneficial to the popularization and application of reagent clinically.
General principle：
In pH in 4.2 cushioning liquid, albumin, can be with anionic dye bromine cresols chloropexia shape as a kind of cation
Into blue-green compound, there is absworption peak at wavelength 600nm, its absorbance is directly proportional to albumin content, with equally handling
Albumin standard is made comparisons, can in the hope of Human Serum Albumin content.
What the present invention was obtained through the following steps：
A kind of seralbumin detection kit, it is characterised in that include reagent R.
Reagent R is constituted：80mmol/L pH are 4.2 succinate buffer, 0.05mmol/L bromocresol greens, 1%
TritonX-100,0.5g/L polyoxyethylene laurel ether, 50mmol/L sodium chloride, 20mmol/L Sodium azides.
Described seralbumin detection kit, it is characterised in that the 1%TritonX-100 is surfactant.
Described seralbumin detection kit, it is characterised in that described 50mmol/L sodium chloride is stabilizer.
Described seralbumin detection kit, it is characterised in that use performance rate method using automatic clinical chemistry analyzer
It is measured, detection dominant wavelength is 600nm.
The kit of the present invention is carried out on the automatic clinical chemistry analyzer with double reagent function, its specifically used method
Such as Fig. 1：
Physiological saline, sample or the μ l of calibration object 3 are added, 300 μ l reagent R is added, absorbance A 1 is read, is incubated after 3min
Absorbance A 2 is read, Δ A is calculated.
Beneficial effects of the present invention：
1）This reagent with the addition of sodium chloride and 1%TritonX-100, enhance the stability of reagent, and do not have to the degree of accuracy of reagent
There is generation influence；
2）The degree of accuracy of reagent and have good stability, it is cheap, it is easy to use, be conducive to the reagent further in the market
Promote.
Brief description of the drawings
Fig. 1 is the trial method of kit；
Fig. 2 is the correlation curve figure of two kinds of reagents；
Fig. 3 is the seralbumin detection reagent contrasting detection result that the reagent of embodiment 1 is approved with market；
Fig. 4 is the reagent linear correlation confirmatory experiment testing result of embodiment 1；
Fig. 5 is the reagent stability confirmatory experiment testing result of embodiment 1.
Embodiment
The present invention is further described with reference to specific embodiment：
Embodiment 1
A kind of conventional seralbumin detection kit, reagent set turns into：
80mmol/L succinate buffers pH 4.2
Bromocresol green 0.05mmol/L
Polyoxyethylene laurel ether 0.5g/L
Sodium chloride 50mmol/L
TritonX-100 1%
Sodium azide 20mmol/L
Reagent R described in the present embodiment, need to first prepare succinate buffer, be transferred to NaOH after proper pH value, then add during configuration
Other materials.When in use, its assay method is complete using advanced in years auspicious 800 with double reagent function to kit described in the present embodiment
Automatic biochemistry analyzer, is measured using end-point method, is operated as follows：
Physiological saline, sample or the μ l of calibration object 3 are added, 300 μ l reagent R is added, absorbance A 1 is read, is incubated after 3min
Absorbance A 2 is read, Δ A is calculated；
Δ A=A2- A1
Serum albumin levels（g/L）=（A determines ÷ A standards）× standard concentration
A is determined：Determine sample absorbance change A standards：Standard items absorbance change.
Embodiment 2
Accuracy validation is tested：Using the seralbumin detection reagent of embodiment 1 as experimental group, in the market obtains the one of accreditation
Plant the seralbumin detection reagent that the degree of accuracy is good, stability is good to be detected as a control group, to 20 clinical serum samples
Detected, testing result is as shown in Figure 3.Obtain the correlation curve of two kinds of reagents（As shown in Figure 2）, as a result show, two
The coefficient correlation of group reagent box is 0.9945, illustrates that both correlations are relatively good.Prove the component pair of kit addition of the present invention
Its accuracy does not result in influence, and kit still keeps the preferable degree of accuracy.
Embodiment 3
Linear dependence checking test：The high level sample that a serum albumin levels are 60g/L is chosen, is entered with physiological saline
Row be serially diluted, prepare 6 various concentrations sample, concentration be followed successively by 60g/L, 48g/L, 36g/L, 24g/L, 12g/
L、0g /L.It is utilized respectively the reagent of embodiment 1 and control group reagent is detected that the sample of each concentration is determined three times respectively, point
Do not average, testing result is as shown in Figure 4：
As shown in figure 4, embodiment 1 is all higher than 0.990 with compareing group reagent testing result coefficient correlation, and the reagent of embodiment 1 is examined
Coefficient correlation of the coefficient correlation slightly larger than control group reagent testing result of result is surveyed, it is more preferable that this shows that reagent of the present invention has
Linear dependence.
Embodiment 4
Stability confirmatory experiment：The store reagents in 2 DEG C~8 DEG C, the light protected environment of non-corrosiveness gas, detection embodiment 1 and
Compare the stability of group reagent.Monthly No. 1 determines Quality Control sample with two group reagents respectively（Target value is 42.4g/L）, determine three times
Average, detection data are as shown in Figure 5：
Experimental result shows that the reagent of embodiment 1 stores 15 months surely in 2 DEG C~8 DEG C, the light protected environment of non-corrosiveness gas
It is fixed, and compare group reagent and store in 2 DEG C~8 DEG C, the light protected environment of non-corrosiveness gas and start within 12 months unstable, illustrate
Invention reagent adds sodium chloride and the enhancing of TritonX-100 stability.
In summary, addition sodium chloride and TritonX- in the seralbumin detection kit that the present invention is provided, reagent R
100, the stability of kit is improved, preferably, the degree of accuracy of reagent is also preferable for the range of linearity.Therefore, the blood that the present invention is provided
Pure protein detection kit is conducive to further promoting the use of in the market.
Claims (1)
1. a kind of seralbumin detection kit, only comprising reagent R, it is characterised in that the reagent composition is as follows：
80mmol/L pH are 4.2 succinate buffer, 0.05mmol/L bromocresol greens, 1% TritonX-100,0.5g/L gather
Oxygen ethene bay ether, 50mmol/L sodium chloride, 20mmol/L Sodium azides.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710237555.1A CN107064527A (en) | 2017-04-12 | 2017-04-12 | A kind of seralbumin detection kit |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710237555.1A CN107064527A (en) | 2017-04-12 | 2017-04-12 | A kind of seralbumin detection kit |
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| CN107064527A true CN107064527A (en) | 2017-08-18 |
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| CN201710237555.1A Pending CN107064527A (en) | 2017-04-12 | 2017-04-12 | A kind of seralbumin detection kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108333364A (en) * | 2018-01-10 | 2018-07-27 | 三诺生物传感股份有限公司 | It is a kind of to detect sero-abluminous reagent and its application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1056750A (en) * | 1991-07-15 | 1991-12-04 | 中国人民解放军302医院 | A kind of reagent that seralbumin uses and preparation method thereof of measuring |
| CN102628864A (en) * | 2011-12-30 | 2012-08-08 | 北京九强生物技术股份有限公司 | Kit for determining heart-type fatty acid binding protein in serum or urine by latex enhanced turbidimetric immunoassay |
| CN103278648A (en) * | 2013-05-24 | 2013-09-04 | 宁波美康生物科技股份有限公司 | Albumin detection reagent |
| CN104198471A (en) * | 2014-08-14 | 2014-12-10 | 苏州康铭诚业医用科技有限公司 | Surface active agent for determination of human serum albumin |
| CN106442352A (en) * | 2016-09-24 | 2017-02-22 | 济南中安生物技术服务有限公司 | Total serum protein detection kit with strong anti-interference capability |
-
2017
- 2017-04-12 CN CN201710237555.1A patent/CN107064527A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1056750A (en) * | 1991-07-15 | 1991-12-04 | 中国人民解放军302医院 | A kind of reagent that seralbumin uses and preparation method thereof of measuring |
| CN102628864A (en) * | 2011-12-30 | 2012-08-08 | 北京九强生物技术股份有限公司 | Kit for determining heart-type fatty acid binding protein in serum or urine by latex enhanced turbidimetric immunoassay |
| CN103278648A (en) * | 2013-05-24 | 2013-09-04 | 宁波美康生物科技股份有限公司 | Albumin detection reagent |
| CN104198471A (en) * | 2014-08-14 | 2014-12-10 | 苏州康铭诚业医用科技有限公司 | Surface active agent for determination of human serum albumin |
| CN106442352A (en) * | 2016-09-24 | 2017-02-22 | 济南中安生物技术服务有限公司 | Total serum protein detection kit with strong anti-interference capability |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108333364A (en) * | 2018-01-10 | 2018-07-27 | 三诺生物传感股份有限公司 | It is a kind of to detect sero-abluminous reagent and its application |
| CN108333364B (en) * | 2018-01-10 | 2020-05-19 | 三诺生物传感股份有限公司 | Reagent for detecting serum albumin and application thereof |
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