CN107064441A - The method that quality of mutton is evaluated based on the labelled protein that phosphated peptide section is screened - Google Patents
The method that quality of mutton is evaluated based on the labelled protein that phosphated peptide section is screened Download PDFInfo
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/12—Meat; Fish
Landscapes
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a kind of method for evaluating quality of mutton based on the labelled protein that phosphated peptide section is screened, this method is by tree species for bio-energy source to height, the phosphorylation level of peptide fragment is carried out qualitatively and quantitatively in low-quality mutton, and GO and KEGG enrichment analyses are carried out to the phosphorylated protein after qualitative, pass through protein bio information analysis phosphorylated protein and the potential relation of quality of mutton, by protein phosphorylation level in height, the phosphorylated protein with notable difference is used as labelled protein in low-quality mutton, so as to judge the quality of quality of mutton according to the content of mutton marker protein.Phosphorylated protein in first identified of the present invention mutton, labelled protein for screening reflection quality of mutton provides foundation, and can be according to the horizontal result judgement quality of mutton of protein phosphorylation, there is provided a kind of new method for evaluating quality, be conducive to creating more profits for meat enterprise, high quality and favourable price is realized, is had a extensive future.
Description
Technical field
The invention belongs to food quality detection technical field, and in particular to one kind is based on phosphated peptide section screening reflection meat
The labelled protein of matter, the method that different quality of mutton are evaluated using the labelled protein.
Background technology
In quality of mutton evaluation, the content of main component in mutton is mainly detected, at present new fresh mutton main component
In the detection technique of content, many national most common methods are still traditional chemical analysis, and this method has detection speed
Degree is relatively slow, it is necessary to which instrument and equipment is more and complicated, and cost cost is higher, and detection process is the shortcomings of can destroy sample, it is difficult to accomplish
To the on line real-time monitoring of new fresh mutton.In order to overcome the shortcomings of in conventional method, meat is detected using near infrared spectroscopic method
Quality and degree of metamorphism are a selections well, and it has, and detection speed is fast, excessive pretreatment need not be done to sample, be can be achieved
The advantages of nondestructive measurement, the meat quality detection based on near-infrared spectral analysis technology was also once becoming one during meat is evaluated
Individual hot topic, and continue till now.
However, during near infrared technology Non-Destructive Testing quality of mutton, it is necessary to set up detection model, it is necessary to substantial amounts of sample,
And the requirement to sample is high.In view of conventional method and near-infrared method are all to study meat in terms of the apparent and chemical substance
The change of matter, the identification of meat fails to go deep into explanation mechanism, and influences the factor of quality of mutton relatively more, and postmortem aging,
The structure of protein and functional characteristic are more notable to the qualitative effects of meat and meat products in carcass cutting position and muscle.After government official
Muscle cell metabolism in maturation largely decides the quality characteristic of meat, such as pH, tenderness, color and luster and retentiveness
Deng meat quality.And the most of myosinogen, fribrillin in muscle are by protein phosphorylation and dephosphorylation mistake
Journey influences, therefore phosphorylated protein can be used as the protein labeling for reflecting quality of mutton.Meat quality evaluation index mainly has pH
Value, shearing force, yellowish pink, retentiveness etc..These indexs are all the macroscopic view reflections of micro-variations in muscle cell, and protein is as thin
The row envoy of born of the same parents' vital movement function, its phosphorylation modification can cause a series of physiological acoustic signals in muscle cell, so that shadow
Ring meat after killing.Therefore key protein phosphorylation modification and its phosphorylation level change can be used as reflection meat quality in muscle
Mark.
The content of the invention
It is difficult to go deep into the deficiency that meat change mechanism and existing quality of mutton are graded for current mutton evaluation method,
It is an object of the invention to provide it is a kind of based on phosphated peptide section quantitative and qualitative screen reflection quality of mutton labelled protein and with
The method that the labelled protein evaluates quality of mutton.
The used technical scheme that solves the above problems is made up of following step：
1st, the high-quality mutton of 12 hours and low-quality meat samples after collection is killed.
2nd, the phosphorylated protein in the collection of extraction step 1 sample, and the phosphorylated protein of acquisition is digested, obtains phosphorylation
Peptide fragment.
3rd, the phosphated peptide section obtained to step 2 using biological mass spectrometry carries out qualitative and quantitative detection, with the phosphorus after qualitative
It is acidified the logarithm value conduct of relative quantification value of the peptide fragment in high-quality mutton and the ratio between the relative quantification value in low-quality mutton
Judge that the foundation of peptide fragment phosphorylation level change, the i.e. logarithm value are more than 1 phosphorylation level up-regulation, the logarithm value is less than -1
Phosphorylation level lower, the logarithm value be between 1 and -1 then the phosphated peptide section in high-quality mutton and low-quality mutton sample
Phosphorylation level in product is without significant changes.
4th, the corresponding phosphorylated protein of phosphated peptide section to step 3 after qualitative carries out GO and KEGG enrichment analyses.
5th, it is according to the analysis result of step 4, content difference in high-quality meat samples and low-quality meat samples is notable
Phosphorylated protein be used as labelled protein.
6th, the content of different meat samples marker proteins to be measured is detected, different mutton are judged according to the content of labelled protein
Between quality height：If labelled protein is high-quality mutton labelled protein, the higher explanation sheep to be measured of labelled protein content
Meat sample quality is better；If labelled protein is low-quality mutton labelled protein, the higher explanation sheep to be measured of labelled protein content
Meat sample quality is poorer.
In above-mentioned steps 1, the high-quality mutton is the longissimus dorsi muscle meat of sheep, and low-quality mutton is the abdominal muscles of sheep.
In above-mentioned steps 3, the detection includes the detection of peptide fragment phosphorylation site and phosphorylation level.
In above-mentioned steps 5, the relative quantification value of phosphated peptide section and low high-quality mutton in the high-quality meat samples
The logarithm value of the ratio between relative quantification value of phosphated peptide section is more than 1 or less than -1 in sample, then the phosphated peptide section is in high-quality
Content difference in meat samples and low high-quality mutton is notable.
The labelled protein of above-mentioned high-quality mutton is phosphofructokinase, triphosphoric acid isomerase 1, pyruvate kinase, phosphorus
Acid glycerol acid kinase 1, fructosediphosphate aldolase A and glyceraldehyde-3-phosphate dehydrogenase, the labelled protein of low-quality mutton is original
Myosin 1, myoglobulin heavy chain 1, myoglobulin heavy chain 2, myoglobulin heavy chain 6 and titin.
The present invention passes through to (the high-quality mutton by representative of longissimus dorsi muscle and with abdominal muscles of high low-quality mutton after government official
For the low-quality mutton of representative) in phosphorylated protein extracted and digested, then using biological mass spectrometry to the phosphorus after enzymolysis
Be acidified peptide fragment carry out qualitative and quantitative analysis, and to after government official mutton macroscopic view quality changes (mutton pH value, shearing force, color and luster,
Protein solubility, retentiveness) measure, then will be qualitative after phosphorylating protein carry out GO and KEGG enrichment analysis, root
According to protein bio information analysis phosphorylating protein and the potential relation of quality of mutton, by protein phosphorylation level (with egg
The mass spectrum response signal value of the corresponding phosphated peptide section of white matter as protein phosphorylation level basis for estimation) height it is low-quality
Then the phosphorylated protein with notable difference integrates the GO of meat samples marker protein to be measured as labelled protein in mutton
Analysis result is enriched with KEGG, the specific quality of mutton that the albumen is reflected is judged.With existing quality of mutton authentication method phase
Than the present invention has the advantages that：
1st, phosphorylating protein in first identified of the present invention mutton, the protein labeling for reflecting the quality of mutton for screening is carried
Foundation is supplied.
2nd, quality of mutton is classified after the present invention is for killing, can be according to the horizontal result judgement mutton product of protein phosphorylation
Matter.
3rd, the present invention has a extensive future, and grades for quality of mutton control and the mutton and has great importance,
Even there is directive significance to the height quality evaluation of beef.
4th, after the present invention is by high and low quality grading, is conducive to creating more profits for meat enterprise, realizes that high-quality is excellent
Valency.
5th, the present invention can be deep into molecule and gene level, embody meat change mechanism, so as to understand meat in depth
Quality comparison.
Brief description of the drawings
Fig. 1 is phosphated peptide section quantitative result distribution map.
Fig. 2 is phosphorylated protein GO classification results figures.
Fig. 3 is phosphorylated protein KEGG enrichment result figures.
Fig. 4 is Protein interaction mapping in high-quality mutton, and wherein APOBEC2 is that non-carrier B messenger rna editing enzymeses are urged
Change subunit 2, PKM is pyruvate kinase, and TPI1 is triphosphoric acid isomerase 1, and PFKM is phosphofructokinase, and TWF2 is mariages albumen
Associated proteins 2, ZC3HC1 is the zinc finger protein of type containing C3HC 1, and GAPDH is glyceraldehyde-3-phosphate dehydrogenase, and PGK1 is that phosphoric acid is sweet
Oily kinases 1, ALDOA is fructosediphosphate aldolase A, and HERC1 is HECT domain+E3 ubiquitin ligases family protein 1,
EIF4EBP1 is eukaryotic translation initiation factor 4E Binding Protein 1s, and EIF4G1 is the gamma 1 of eukaryotic translation startup factor 4, and HSPA1A is
Heat-shock protein family A, HSP90AA1 are Heat shock protein72 family A1 subunits, and PPP1R3A is that protein phosphatase 1 regulates and controls Asia
Base 3A, CAMK2A are the α of cam kinase 2, and MUSTN1 is embryonic nucleus albumen 1, and ACY1 is the antibody of amino-acylase 1, ALDH1A2
For the family A2 of aldehyde dehydrogenase 1, PEA15 is the phosphoprotein enriched in astroglia, and POLR2M is rna plymerase ii subunit M,
PDLIM3 is PDZ and LIM domains 3, and MYH7 is myoglobulin heavy chain 7, and VCL is vinculin, and TPM1 is tropomyosin 1, and TCAP is
Titin.
Fig. 5 is Protein interaction mapping in low-quality mutton, and wherein HSPB6 is that heat-shock protein family B6, ATP5B are
ATP synzyme, H+ transhipments, mitochondria F1 complexs, β peptides, MUSTN1 are embryonic nucleus albumen 1, and MYH1 is myoglobulin heavy chain 1,
TPM1 is tropomyosin 1, and TTN is titin, and MYOZ1 is myogen regulatory protein 1, and MYOT is flesh contractile protein, and BAG3 is
Bcl-2 combinations anti-apoptotic proteins 3, HSP90AA1 is Heat shock protein72 family A1 subunits, and MYH2 is myoglobulin heavy chain 2,
MYH6 is myoglobulin heavy chain 6, and ANXA2 is ANX2L4, and CLIP1 is cytoplasmic linker proteins 1, and NCBP1 combines for core cap
Protein protomer 1, ATP2A2 is myoplasm net type calcium ion ATP enzyme 2, and KIF1C is driving albumen 1C types, and MYOM3 is myosinogen 3,
ARPP19 is that recombined human cAMP adjusts phosphoprotein 19, and XRN1 is 5'-3'RNA excision enzymes 1, and STIM1 is stromal interaction molecule 1,
PSMB5 is proteasome subunit β 5, and PGM1 is that phosphoric acid reconciles enzyme 1, and TWF2 is mariages protein-binding protein 2.
Embodiment
The present invention is described in more detail with reference to the accompanying drawings and examples, but protection scope of the present invention is not limited only to
These embodiments.
Embodiment 1
1st, choose that body weight is close, feeding manner identical Northern Shaanxi White Cashmere Goat 10 (ram, it is not dead, body weight 23.12 ±
1.62kg), stop eating before government official 20h, cut off the water 2h, after government official by trunk hanging be placed in 4 DEG C of acid discharges.Take its back of the body most long respectively at 12h after government official
Muscle as high-quality meat samples, take its abdominal muscles as low-quality meat samples.
2nd, the high-quality meat samples and each 1g of low-quality meat samples of step 1 are weighed, are respectively placed in 10mL centrifuge tubes,
6mL cushioning liquid (including 7M urea, 2M thiocarbamides, 0.1%CHAPS, inhibitors of phosphatases, protease inhibitors) is added, whirlpool is used
Revolve oscillator vortex to mix, then crush and be homogenized 3 times, each 60s, amplitude 22% using ultrasonic cell disruption instrument, be homogenized
Room temperature places 30min afterwards and 15000r/min centrifuges 20min under the conditions of 4 DEG C, collects supernatant；Supernatant is placed in centrifuge tube
In, add the 100 μ L10mM DTT aqueous solution in centrifuge tube, and be placed in constant-temperature incubation device 60 DEG C and react 1 hour, then
The 40mM IAA aqueous solution that 40 μ L are newly prepared is added, is reacted at room temperature 10 minutes.After the completion of above-mentioned reaction, 10K is transferred the solution into
Super filter tube in, in 12000r/min centrifuge 20 minutes, discard solution in collecting pipe, into super filter tube add trypsase (pancreas
Protease is 1 with Proteins In Aqueous Solutions mass ratio:50) 37 DEG C of enzymolysis 12h in couveuse, and by super filter tube are placed into.Will enzymolysis
Solution 12000r/min afterwards is centrifuged 20 minutes, obtains phosphated peptide section, is freezed.
3rd, the phosphated peptide section for freezing 0.2mg (contains 5%TFA, 1M hydroxyacetic acid and 20% with 100 μ L cushioning liquid
The ACN aqueous solution) after dissolving, 1.2mg TiO are added in the solution2(every 100 μ g phosphated peptide sections add 0.6mg TiO2) simultaneously
It is placed in 600r/min on shaking table and vibrates 10min, then 3000r/min centrifuges 10min, collects supernatant and add 1mg TiO2Again
Secondary be placed in shakes centrifugation after bed reaction 10min, abandons supernatant.Merge centrifugation gained TiO twice2Precipitation.By the TiO being collected into2It is heavy
Shallow lake is mixed after 1min with 100 μ L cushioning liquid (aqueous solution containing 5%TFA, 1M hydroxyacetic acid and 20%ACN) first,
3000r/min centrifuge 10min, gained precipitation with 100 μ L cushioning liquid (containing 80% and 1%TFA the aqueous solution) clean after again from
The heart, gained precipitation is centrifuged again after being cleaned with 100 μ L cushioning liquid (aqueous solution for containing 20% and 0.2%TFA), is collected precipitation, is used
50 μ L eluents (contain 28%NH3·H2The O aqueous solution) it will be adsorbed in precipitation in TiO2On phosphated peptide section elute, receive
Collect eluent, freeze, obtain the phosphated peptide section of enriching and purifying.
With 20 μ L methanol and formic acid volume ratio it is 20 by the phosphated peptide section of above-mentioned enriching and purifying:After 1 mixed liquor dissolving
12000r/min is centrifuged 10 minutes, Aspirate supernatant, combines Easy nLC1000 efficient liquid phases using Q-Exactive mass spectrographs
Series LC-the MSn (EASY-Spray column, 12cm × 75m, C18,318) of composition is analyzed supernatant, chromatostrip
Part：Mobile phase A：100% ultra-pure water, 0.1% formic acid, Mobile phase B：100% acetonitrile, 0.1% formic acid, load flow rate pump：
350nL/min, 15 minutes, separates flow velocity：350nL/min, loading volume：10 μ L, flowing phase separation gradient is as shown in table 1 below.
Table 1 flows phase separation gradient
The mass spectrum original document of generation use MaxQuant1.5 software processings, database used in protein retrieval be from
The Ovis aries databases obtained in NCBI, using FDR as 1% filtering false positive.Respectively to high-quality mutton and low-quality sheep
Identify that the relative quantification value of obtained all phosphated peptide sections does homogenization processing in meat sample product, phosphated peptide section is sought afterwards in height
The logarithm value of relative quantification value and the ratio between the relative quantification value in low-quality meat samples in quality meat samples is used as judgement
The foundation of peptide fragment phosphorylation level change, the i.e. logarithm value are more than 1 phosphorylation level up-regulation, and the logarithm value is less than -1 phosphoric acid
Change horizontal down-regulation, the logarithm value be between 1 and -1 then the phosphated peptide section in high-quality mutton and low-quality meat samples
Phosphorylation level without significant changes.Analysis result is shown in Fig. 1.
As shown in figure 1, identification obtains 2125 phosphated peptide sections altogether in high-quality mutton and low-quality meat samples, these
Peptide fragment belongs to 750 phosphorylated proteins.Wherein phosphorylation level raises peptide fragment 387, and phosphorylation level lowers peptide fragment 581,
Peptide fragment of the phosphorylation level without significant changes 1157, they account for 18%, 27% and the 55% of total peptide segment number respectively.Phosphoric acid
The extremely significant albumen of change level difference in two groups of samples mainly has：Phosphofructokinase (PFKM), pyruvate kinase (PKM),
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), triphosphoric acid isomerase 1 (TPI1), fructose diphosphate aldehyde lyase A (ALDOA), flesh ball
Albumen (MYH1, MYH2 and MYH6), tropomyosin 1 (TPM1), titin (TCAP), myosin light chain (MYL2), flesh
Calcium protein I (troponin I), myoplasm net type calcium ion ATP enzyme 2 (ATP2A2), heat shock protein (HSP27 and HSP70-1A/
B) etc..
4th, the phosphorylated protein detected using blast2GO2.8 softwares to step 3 carries out GO enrichments, as a result sees Fig. 2, and
Using online DAVID software analysis phosphorylated protein KEGG paths, Fig. 3 is as a result seen.
As shown in Fig. 2 learnt by the bioprocess analysis participated in phosphorylated protein, what phosphorylated protein was primarily involved in
Bioprocess includes bioprocess and adjusts (Active Regulation of such as enzyme, GO:0050790), intracellular bioprocess (such as phosphorylation
Reaction, GO:0016310), metabolic process (GO:0008152) (such as cynapse signal transduction, GO are transmitted with signal:0007268)；It is logical
Cross the cellular localization analysis to phosphorylated protein and learn that phosphorylated protein is primarily present in cytoplasm (GO:0005737), it is located at
Sarcoplasmic reticulum (the GO of cell membrane:0033017) with (such as mitochondria, GO in organelle:0005739)；By to phosphorylated protein
Functional analysis learns that most of phosphorylated proteins have binding function (such as to be combined with actin；GO:0003779), catalytic activity
(such as protein kinase activity；GO:0016301), transport activity (such as oxygen transportation, GO:And the function point analysis factor 0005344)
(such as atriphos zymoexciter, GO:0001671).
As can be seen from Figure 3,750 phosphorylated proteins take part in 23 paths, wherein carbohydrate metabolism, amino acid generation
Thank, signal transduction and lipid-metabolism etc. are that KEGG is enriched with significant metabolic pathway.
STRING database protein-protein interaction networks are built by online database, factor data bank lacks related
The Protein Information of Ovis aries species, therefore using the Bos taurus albumen databases for belonging to same section with it.
As shown in figure 4, participating in enzyme such as phosphofructokinase (PFKM), the triphosphoric acid isomerase of glycolysis in high-quality mutton
1 (TPI1), pyruvate kinase (PKM), phosphoglyceric kinase 1 (PGK1), fructosediphosphate aldolase A (ALDOA) and glycerine
Aldehyde -3- phosphate dehydrogenases (GAPDH) constitute the core of the protein-protein interaction network, and these albumen can also be used as high-quality
The labelled protein of mutton.
As shown in figure 5,24 are had in low-quality mutton the complicated phosphorylated protein interacted, in the protein phase
In interaction network, muscle skelemin such as tropomyosin 1 (TPM1), myoglobulin heavy chain 1 (MYH1), myosin weight
The interaction of chain 2 (MYH2), myoglobulin heavy chain 6 (MYH6) and titin (TTN) constitutes the core of the network, these
Albumen also can as low-quality mutton labelled protein.
6th, the series LC-MSn of Easy nLC1000 efficient liquid phases composition is combined according to upper using Q-Exactive mass spectrographs
The method for stating step 2~3 detects the content of different meat samples marker proteins to be measured, can be sentenced according to the content of labelled protein
Quality height between disconnected different mutton：If labelled protein is high-quality mutton labelled protein, labelled protein content is higher
Illustrate that meat samples quality to be measured is better；If labelled protein is low-quality mutton labelled protein, labelled protein content is higher
Illustrate that meat samples quality to be measured is poorer.
Claims (6)
1. a kind of method that quality of mutton is evaluated based on the labelled protein that phosphated peptide section is screened, it is characterised in that by following steps
Composition：
(1) the high-quality mutton of 12 hours and low-quality meat samples after collection is killed；
(2) phosphorylated protein in extraction step (1) collection sample, and the phosphorylated protein of acquisition is digested, obtains phosphorylation
Peptide fragment；
(3) the qualitative and quantitative detection of phosphated peptide section progress obtained to step (2) using biological mass spectrometry, with the phosphoric acid after qualitative
Change the logarithm value of relative quantification value of the peptide fragment in high-quality mutton and the ratio between the relative quantification value in low-quality mutton as sentencing
The foundation of disconnected peptide fragment phosphorylation level change, the i.e. logarithm value are more than 1 phosphorylation level up-regulation, and the logarithm value is less than -1 phosphorus
Be acidified horizontal down-regulation, the logarithm value be between 1 and -1 then the phosphated peptide section in high-quality mutton and low-quality meat samples
In phosphorylation level without significant changes；
(4) the corresponding phosphorylated protein of phosphated peptide section after qualitative to step (3) carries out GO and KEGG enrichment analyses；
(5) it is according to the analysis result of step (4), content difference in high-quality meat samples and low-quality meat samples is significant
Phosphorylated protein is used as labelled protein；
(6) detect the content of different meat samples marker proteins to be measured, according to the content of labelled protein judge different mutton it
Between quality height：If labelled protein is high-quality mutton labelled protein, the higher explanation mutton to be measured of labelled protein content
Sample quality is better；If labelled protein is low-quality mutton labelled protein, the higher explanation mutton to be measured of labelled protein content
Sample quality is poorer.
2. the method according to claim 1 for evaluating quality of mutton based on the labelled protein that phosphated peptide section is screened, it is special
Levy and be：In step (1), the high-quality mutton is the longissimus dorsi muscle meat of sheep, and low-quality mutton is the abdominal muscles of sheep.
3. the method according to claim 1 for evaluating quality of mutton based on the labelled protein that phosphated peptide section is screened, it is special
Levy and be：In step (3), the detection includes the detection of peptide fragment phosphorylation site and phosphorylation level.
4. the method according to claim 1 for evaluating quality of mutton based on the labelled protein that phosphated peptide section is screened, it is special
Levy and be：In step (5), the relative quantification value of phosphated peptide section and low high-quality mutton sample in the high-quality meat samples
The logarithm value of the ratio between relative quantification value of phosphated peptide section is more than 1 or less than -1 in product, then the phosphated peptide section is in high-quality sheep
Content difference in meat sample product and low high-quality mutton is notable.
5. the method according to claim 1 for evaluating quality of mutton based on the labelled protein that phosphated peptide section is screened, it is special
Levy and be：In step (5), the labelled protein of described high-quality mutton is phosphofructokinase, triphosphoric acid isomerase 1, third
Pyruvate kinase, phosphoglyceric kinase 1, fructosediphosphate aldolase A and glyceraldehyde-3-phosphate dehydrogenase.
6. the method according to claim 1 for evaluating quality of mutton based on the labelled protein that phosphated peptide section is screened, it is special
Levy and be：In step (5), the labelled protein of described low-quality mutton is tropomyosin 1, myoglobulin heavy chain 1, flesh ball
Ferritin heavy chain 2, myoglobulin heavy chain 6 and titin.
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