CN107064438A - A kind of method of pseudomonad rapid screening in fresh White mushroom - Google Patents
A kind of method of pseudomonad rapid screening in fresh White mushroom Download PDFInfo
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- CN107064438A CN107064438A CN201710422530.9A CN201710422530A CN107064438A CN 107064438 A CN107064438 A CN 107064438A CN 201710422530 A CN201710422530 A CN 201710422530A CN 107064438 A CN107064438 A CN 107064438A
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- China
- Prior art keywords
- pseudomonad
- white mushroom
- fresh white
- fresh
- rapid screening
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/025—Fruits or vegetables
Abstract
The present invention relates to a kind of method of pseudomonad rapid screening in fresh White mushroom, belong to agricultural product security detection field.This method detects the volatile materials in fresh White mushroom based on smell fingerprint technique, the edible mushroom of different storage times is distinguished by electronic nose principal component analysis, pseudomonad total plate count in fresh food bacterium is estimated by minimal linear regression analysis, the quick detection of pseudomonad in fresh White mushroom is achieved in.Theories integration and settling mode that the present invention provides for quick detection microorganism, have opened up the new approaches to microorganism rapid screening in fresh food bacterium.
Description
Technical field
The present invention relates to a kind of method of pseudomonad rapid screening in fresh White mushroom, belong to agricultural product security detection neck
Domain.
Background technology
White mushroom is of high nutritive value because of its delicious flavour, medicinal and health value, and is favored by consumer, market
On White mushroom it is many sold with fresh goods, and generally preserve at normal temperatures.White mushroom contains abundant protein and amino acid and table
Covering weave is fresh and tender, is more easy to grow inoculating microbe, the species and abundance of outer derived bacterium are in dynamic change in shelf life.
As White mushroom yield rapidly increases, new detection technique is needed in industry to ensure its security, electronic nose is
There is not correlation technique also through being widely used in field of food safety, but in fresh White mushroom.Traditional microorganism detection method
Complex pretreatment, reagent consumption is big, and bacterium colony cultivation cycle is long, it is impossible to which substantial amounts of sample is detected in real time.Modern biotechnology
The microorganism detection method of technology includes：Nucleic Acid Probe Technique, PCR technologies, biochip technology, biology sensor detection skill
Art etc., operating procedure is complicated, and testing cost is high.Gas chromatography measurement result is to unimolecule rank, it is impossible to obtain sample overall
Information, excessively fine result can increase the difficulty for extracting information.
The domestic total plate count to pseudomonad in White mushroom is determined usually using selective medium culture sample at present
Rough colony counts are obtained, or carry out Accurate Determining using round pcr.But existing detection technique is all difficult to a large amount of
The quick detection examination of sample, and operating procedure is complicated, detection cycle is long.Detection in safety verification to pseudomonad is simultaneously not required to
Want accurate colony counts, it is allowed to certain error.
The content of the invention
In view of the above-mentioned problems, the present invention proposes a kind of quick screening method, detected based on smell fingerprint technique fresh
Volatile materials in White mushroom, the pseudomonad total plate count in fresh food bacterium is estimated by minimal linear regression analysis,
It is achieved in the quick detection of pseudomonad in fresh White mushroom.
The present invention sets up electronic nose PLS analyses and pseudomonad Growth trends by predicting hygienic analysis method
The relative error R of mathematical modeling, predicted value and measured value2=0.9379, institute's established model can be predicted preferably in fresh food bacterium
Pseudomonad upgrowth situation.
Concrete operations are：The fresh White mushroom samples of 2g are taken to be placed in 20 mL headspace sampling bottle, polytetrafluoroethylene (PTFE) silicon diaphragm
Stand after 20 min, balance and to be measured using the electric nasus system equipped with 12 kinds of mos sensors after sealing, analytical parameters：Stream
Speed 150 mL/min, the s of head space generation time 60, produce 60 DEG C of temperature, the r/min of stir speed (S.S.) 200, head space volume injected 0.5
ML, the mL/s of injection rate 0.25, obtain the s of time 360, the s of lag time 120.
The forecast model of pseudomonad total plate count is in fresh White mushroom：y = 1.0667x - 0.14.
Wherein, y is pseudomonad total plate count（×105 CFU/g）, x is fresh White mushroom sample Electron nose optimum linear
Sensor response.
The present invention provides green, economic, convenient, easy promote for the pseudomonad quick detection direction in fresh White mushroom
Theories integration and solution route, opened up the new approaches that edible microorganismus high flux fast and safely detects research.
Embodiment
Below in conjunction with instantiation, the invention will be further described.Weigh the head space that White mushroom sample 2g is placed in 20 mL
In sample injection bottle, stood after the sealing of polytetrafluoroethylene (PTFE) silicon diaphragm and the electronics equipped with 12 kinds of mos sensors is used after 20 min, balance
Nasus system is measured, analytical parameters：The mL/min of flow velocity 150, the s of head space generation time 60, produce 60 DEG C of temperature, stirring speed
The r/min of rate 200, the mL of head space volume injected 0.5, the mL/s of injection rate 0.25, obtain the s of time 360, the s of lag time 120.
Thus the response intensity change of fragrance component is shown.
The forecast model of pseudomonad total plate count is in fresh White mushroom：Y=1.0667x-0.14.
Wherein, y is pseudomonad total plate count（×105 CFU/g）, x is fresh White mushroom sample Electron nose optimum linear
Sensor response.
It is 2.35 × 10 to be worth to pseudomonad total plate count predicted value by response5 CFU/g, sample actual value be 2.7 ×
105CFU/g, relative deviation is 12.96%, and the growing state of pseudomonad in fresh White mushroom can be predicted to a certain extent.
Claims (3)
1. a kind of method of pseudomonad rapid screening in fresh White mushroom, it is characterised in that：It is new by electric nasus system analysis
Pseudomonad in fresh White mushroom.
2. the method for pseudomonad rapid screening in fresh White mushroom according to claim 1, it is characterised in that：By sample
It is placed in 20 mL headspace sampling bottle, stands to use after 20 min, balance after the sealing of polytetrafluoroethylene (PTFE) silicon diaphragm and be furnished with 12 kinds
The electric nasus system of mos sensor is measured, analytical parameters：The mL/min of flow velocity 150, the s of head space generation time 60, produce temperature
60 DEG C of degree, the r/min of stir speed (S.S.) 200, the mL of head space volume injected 0.5, the mL/s of injection rate 0.25 obtain the s of time 360,
The s of lag time 120.
3. the method for pseudomonad rapid screening in fresh White mushroom according to claim 1, it is characterised in that：It is fresh
The forecast model of pseudomonad total plate count is in White mushroom：Y=1.0667x-0.14；
Wherein, y is pseudomonad total plate count（×105 CFU/g）, x is fresh White mushroom sample Electron nose optimum linear sensing
Device response.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710422530.9A CN107064438A (en) | 2017-06-07 | 2017-06-07 | A kind of method of pseudomonad rapid screening in fresh White mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710422530.9A CN107064438A (en) | 2017-06-07 | 2017-06-07 | A kind of method of pseudomonad rapid screening in fresh White mushroom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107064438A true CN107064438A (en) | 2017-08-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710422530.9A Pending CN107064438A (en) | 2017-06-07 | 2017-06-07 | A kind of method of pseudomonad rapid screening in fresh White mushroom |
Country Status (1)
| Country | Link |
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| CN (1) | CN107064438A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109030696A (en) * | 2018-07-19 | 2018-12-18 | 南京财经大学 | A kind of method that pseudomonad quickly detects in freeze-drying agaricus bisporus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104267067A (en) * | 2014-10-28 | 2015-01-07 | 南京农业大学 | Method based on smell sensor and used for predicting growth stage of pseudomonas aeruginosa as meat typical putrefying bacterium |
| CN104849323A (en) * | 2015-05-06 | 2015-08-19 | 浙江大学 | Method for quickly detecting clarifying agent in juice based on electronic nose |
-
2017
- 2017-06-07 CN CN201710422530.9A patent/CN107064438A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104267067A (en) * | 2014-10-28 | 2015-01-07 | 南京农业大学 | Method based on smell sensor and used for predicting growth stage of pseudomonas aeruginosa as meat typical putrefying bacterium |
| CN104849323A (en) * | 2015-05-06 | 2015-08-19 | 浙江大学 | Method for quickly detecting clarifying agent in juice based on electronic nose |
Non-Patent Citations (2)
| Title |
|---|
| 刘波等编著: "《食用菌病害及其防治》", 30 April 1991 * |
| 胡慧平等: "应用气味指纹技术检测猪肉假单胞菌", 《食品科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109030696A (en) * | 2018-07-19 | 2018-12-18 | 南京财经大学 | A kind of method that pseudomonad quickly detects in freeze-drying agaricus bisporus |
| CN109030696B (en) * | 2018-07-19 | 2020-09-04 | 南京财经大学 | Method for rapidly detecting pseudomonas in freeze-dried agaricus bisporus |
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Application publication date: 20170818 |