CN107064199B - A kind of application of trans- cucurbit(7)uril identification biogenic amine - Google Patents
A kind of application of trans- cucurbit(7)uril identification biogenic amine Download PDFInfo
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- 150000001412 amines Chemical class 0.000 title claims abstract description 72
- 230000000035 biogenic effect Effects 0.000 title claims abstract description 68
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 claims abstract description 34
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 claims abstract description 32
- PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 24
- 230000009514 concussion Effects 0.000 claims abstract description 19
- 238000005138 cryopreservation Methods 0.000 claims abstract description 19
- 229960001340 histamine Drugs 0.000 claims abstract description 17
- BHHGXPLMPWCGHP-UHFFFAOYSA-N Phenethylamine Chemical compound NCCC1=CC=CC=C1 BHHGXPLMPWCGHP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229940063673 spermidine Drugs 0.000 claims abstract description 16
- 229940063675 spermine Drugs 0.000 claims abstract description 16
- 125000000217 alkyl group Chemical group 0.000 claims description 10
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 6
- 125000002883 imidazolyl group Chemical group 0.000 claims description 3
- -1 tyrasamine Chemical compound 0.000 abstract description 5
- 239000000047 product Substances 0.000 description 60
- 238000004448 titration Methods 0.000 description 15
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical group N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 7
- 238000005160 1H NMR spectroscopy Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 230000005311 nuclear magnetism Effects 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 125000001931 aliphatic group Chemical group 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 235000000832 Ayote Nutrition 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- 241001269238 Data Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- OJGMBLNIHDZDGS-UHFFFAOYSA-N N-Ethylaniline Chemical group CCNC1=CC=CC=C1 OJGMBLNIHDZDGS-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- VTJUKNSKBAOEHE-UHFFFAOYSA-N calixarene Chemical class COC(=O)COC1=C(CC=2C(=C(CC=3C(=C(C4)C=C(C=3)C(C)(C)C)OCC(=O)OC)C=C(C=2)C(C)(C)C)OCC(=O)OC)C=C(C(C)(C)C)C=C1CC1=C(OCC(=O)OC)C4=CC(C(C)(C)C)=C1 VTJUKNSKBAOEHE-UHFFFAOYSA-N 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 150000003983 crown ethers Chemical class 0.000 description 1
- MSBXTPRURXJCPF-DQWIULQBSA-N cucurbit[6]uril Chemical compound N1([C@@H]2[C@@H]3N(C1=O)CN1[C@@H]4[C@@H]5N(C1=O)CN1[C@@H]6[C@@H]7N(C1=O)CN1[C@@H]8[C@@H]9N(C1=O)CN([C@H]1N(C%10=O)CN9C(=O)N8CN7C(=O)N6CN5C(=O)N4CN3C(=O)N2C2)C3=O)CN4C(=O)N5[C@@H]6[C@H]4N2C(=O)N6CN%10[C@H]1N3C5 MSBXTPRURXJCPF-DQWIULQBSA-N 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 150000008040 ionic compounds Chemical class 0.000 description 1
- 150000002678 macrocyclic compounds Chemical class 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 150000007530 organic bases Chemical class 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N24/00—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
- G01N24/08—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects by using nuclear magnetic resonance
- G01N24/088—Assessment or manipulation of a chemical or biochemical reaction, e.g. verification whether a chemical reaction occurred or whether a ligand binds to a receptor in drug screening or assessing reaction kinetics
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- High Energy & Nuclear Physics (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of applications of trans- cucurbit(7)uril identification biogenic amine.It can be used to identify tryptamines, recognition methods is as follows: trans- cucurbit(7)uril is put into nuclear magnetic tube by a., and D is added2O concussion, makes it dissolve, obtains A product；B. tryptamines is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increase of tryptamines amount, a series of nuclear magnetic spectrogram of host-guest complexes is obtained, by it compared with tryptamines nuclear magnetic spectrogram, if occurring two groups of signal peaks Hg and Hf in map, wherein Hg is mobile toward low field, and Hf is mobile toward High-Field, while the signal peak peak type of other Ha-He proton broadens, and it is also mobile toward High-Field, then illustrate that the biogenic amine is tryptamines.The present invention can effectively identify 6 kinds of tryptamines, spermine, spermidine, tyrasamine, histamine and phenyl ethylamine biogenic amines, significant to announcement biological phenomena and process.
Description
Technical field
The present invention relates to supramolecular chemistry field, especially a kind of application of trans- cucurbit(7)uril identification biogenic amine.
Background technique
Melon ring (Cucurbit [n] uril), also known as Cucurbituril, (abbreviation CB [n] or Q [n]) are obtained because structure is like pumpkin
Name, is the novel caged host compound of another class after the macrocyclic compound such as crown ether, cyclodextrin, calixarenes, from structural
From the point of view of in matter, melon ring has the hydrophobic cavity of high degree of symmetry and both ends open and is covered with polar carbonylic oxygen atom,
The feature of this structure can be selective under solution state inclusion size dimension it is suitable organic and inorganic and biological
The small molecules such as molecule act on again or with hydrophilic complex occurs at port with dipole or ionic compound, and from function
From the point of view of in characteristic, melon ring has emphatically in terms of increasing medicine stability as a kind of potential medicament transport, sustained-release and controlled release carrier
The meaning wanted, while with the deep progress of research work, melon ring are applied to organic synthesis gradually, molecular recognition, nano material,
Catalysis, separation etc..Trans- melon ring as in melon ring synthesis process by the intermediate product of dynamics Controlling, in 2005
Isaacs and Kim study group reports for the first time, in the structure of this kind of melon ring, the glycosides urea unit inverted there is one, so that should
Two protons on unit waist are directed toward the cavity of melon ring, but because content is low, and the factors such as hardly possible separation limit the development of trans- melon ring
With application.
Biogenic amine is the general name of a kind of low molecular weight organic compound for having bioactivity nitrogenous, is considered as amino molecule
The substance that middle 1-3 hydrogen atom generates after being replaced by alkyl or aryl, is aliphatic, Zhi Huan race or heterocyclic low molecular weight
Organic base can be divided into this three categories of aliphatic, aromatic series, heterocyclic amine according to its structure.Alkamines compound is normally present in dynamic
In plant and in food, micro biogenic amine has apparent diastole and contraction to cerebration and big blood vessel and muscle
Cortex has important adjustment effect, can accelerate the growth and development of organism simultaneously biogenic amine be also it is a kind of have to a variety of behaviors it is latent
In the neurotransmitter of effect, there is important physiological function to life entity normal activity, we are based on trans- cucurbit(7)uril
Molecule investigates the recognition property between trans- cucurbit(7)uril and biogenic amine by a series of characterization methods, explore Subjective and Objective it
Between binding mode, understood the binding site between them in depth, effect when thermodynamic parameter is pushed further into trans-
Melon ring is to lay the foundation in terms of carrier mass is applied to newtype drug transmission.
Summary of the invention
The object of the present invention is to provide a kind of application of trans- cucurbit(7)uril identification biogenic amine, the present invention can be identified effectively
6 kinds of tryptamines, spermine, spermidine, tyrasamine, histamine and phenyl ethylamine biogenic amines, it is significant to announcement biological phenomena and process, have
Broad application prospect.
The present invention is implemented as follows: a kind of application of trans- cucurbit(7)uril identification biogenic amine, tryptamines, knows for identification
Other method is as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtain a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with tryptamines nuclear magnetic spectrogram, if occurring two groups of letters in map
Number peak Hg and Hf, wherein Hg is mobile toward low field, and Hf is mobile toward High-Field, while the signal peak peak type of other Ha~He proton
It broadens, and also mobile toward High-Field, then illustrates that the biogenic amine is tryptamines.
The application of trans- cucurbit(7)uril identification biogenic amine above-mentioned, is also used to identify spermine, recognition methods is as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtain a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with spermine nuclear magnetic spectrogram, if Hd and He proton in map
Signal peak-to-average power toward low field, mobile, Hc is mobile toward High-Field without apparent motion and Ha and Hb, then illustrate that the biogenic amine is spermine.
The application of trans- cucurbit(7)uril identification biogenic amine above-mentioned, is also used to identify spermidine, recognition methods is as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtain a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with spermidine nuclear magnetic spectrogram, if Hd proton in map
Signal peak is mobile toward low field, and the signal peak-to-average power of Ha, Hb, Hc, He, Hf, Hg proton is mobile to High-Field, then illustrates that the biogenic amine is
Spermidine.
The application of trans- cucurbit(7)uril identification biogenic amine above-mentioned, is also used to identify tyrasamine, recognition methods is as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtain a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with tyrasamine nuclear magnetic spectrogram, if in map on alkyl chain
The signal peak-to-average power of Hc and Hd proton is mobile toward High-Field, if the proton in map on phenyl ring signal peak-to-average power it is mobile toward High-Field,
Then illustrate that the biogenic amine is tyrasamine.
The application of trans- cucurbit(7)uril identification biogenic amine above-mentioned, is also used to identify histamine, recognition methods is as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtain a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with histamine nuclear magnetic spectrogram, if in map on imidazole ring
The signal peak of Ha and Hb proton have the tendency that the signal peak of Hc and Hd proton mobile toward High-Field, on alkyl chain respectively toward High-Field and
Low field is mobile, then illustrates that the biogenic amine is histamine.
The application of trans- cucurbit(7)uril identification biogenic amine above-mentioned, is also used to identify phenyl ethylamine, recognition methods is as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtain a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with phenyl ethylamine nuclear magnetic spectrogram, if in map on alkyl chain
Hd and He proton signal peak-to-average power proton mobile toward low field, on phenyl ring signal peak-to-average power it is mobile toward High-Field, then illustrate the life
Object amine is phenyl ethylamine.
Beneficial effect
Compared with prior art, the present invention can effectively identify tryptamines, spermine, spermidine, tyrasamine, histamine and phenyl ethylamine 6
Kind biogenic amine, it is significant to announcement biological phenomena and process, it is with a wide range of applications.
There is the identification function in order to further verify the present invention, inventor has done isothermal titration calorimetric experiment and mass spectrum
Experiment.
The isothermal titration calorimetric of trans- cucurbit(7)uril iQ [7] and tryptamines is tested:
Tryptamines is made into 1.00 × 10-3The solution of mol/L, iQ [7] are made into 1.00 × 10-4The solution of mol/L is in aqueous solution
It is middle to titrate iQ [7] with tryptamines, using the equilibrium constant of Nano ITC isothermal titration calorimeter measurement iQ [7] and tryptamines at 25 DEG C
And thermodynamic parameter.IQ [7] aqueous solution of addition 1.3mL (0.1mmol/L) in sample cell, 6 μ L/ drop of tryptamines (1.0mmol/L),
Interval time is 250s, mixing speed 250r/min, titrates 30 experimental datas by Nano ITC instrument institute by reference of water
It is as shown in Figure 3 that configuration software Launch Nano Analyze is fitted analysis.The experimental results showed that trans- cucurbit(7)uril and color
Amine forms Subjective and Objective than the supramolecular complex for 1:1.
By above-mentioned same experimental considerations respectively to tryptamines, spermine, spermidine, tyrasamine, histamine, phenyl ethylamine progress isothermal drop
Quantitative heat experiment, experimental result is respectively as shown in Fig. 3, Fig. 7, Figure 13, Fig. 9, Figure 11, Fig. 5.Fig. 3 is it is found that trans- cucurbit(7)uril iQ
[7] Subjective and Objective is formed with tryptamines than the supramolecular complex for 1:1.As shown in Figure 7, trans- cucurbit(7)uril iQ [7] and spermine shape
At Subjective and Objective than the supramolecular complex for 1:1.Trans- cucurbit(7)uril iQ [7] and spermidine are capable of forming host and guest as shown in Figure 13
Body is than the supramolecular complex for 1:1.As shown in Figure 9, it is 1 that trans- cucurbit(7)uril iQ [7] and tyrasamine, which are capable of forming Subjective and Objective ratio:
1 supramolecular complex.As shown in Figure 11, trans- cucurbit(7)uril iQ [7] and histamine are capable of forming Subjective and Objective than the oversubscription for 1:1
Sub- complex compound.As shown in Figure 5, trans- cucurbit(7)uril iQ [7] and phenyl ethylamine form Subjective and Objective than the supramolecular complex for 1:1.
The binding constant that is mutually complexed by observing trans- cucurbit(7)uril and biogenic amine simultaneously, it is also known that equal shape between Subjective and Objective
At stable inclusion complex, this identifies that biogenic amine provides prerequisite for trans- cucurbit(7)uril.
Detailed description of the invention
The structure chart of the trans- cucurbit(7)uril iQ [7] of molecule based on Fig. 1；
Fig. 2 is trans- cucurbit(7)uril iQ [7] and tryptamines1H NMR titration figure (500MHz, D2O)；
Fig. 3 is the isothermal titration calorimetric figure of trans- cucurbit(7)uril iQ [7] and tryptamines；
Fig. 4 is trans- cucurbit(7)uril iQ [7] and phenyl ethylamine1H NMR titration figure (500MHz, D2O)；
Fig. 5 is the isothermal titration calorimetric figure of trans- cucurbit(7)uril iQ [7] and phenyl ethylamine；
Fig. 6 is trans- cucurbit(7)uril iQ [7] and spermine1H NMR titration figure (500MHz, D2O)；
Fig. 7 is the isothermal titration calorimetric figure of trans- cucurbit(7)uril iQ [7] and spermine；
Fig. 8 is trans- cucurbit(7)uril iQ [7] and tyrasamine1H NMR titration figure (500MHz, D2O)；
Fig. 9 is the isothermal titration calorimetric figure of trans- cucurbit(7)uril iQ [7] and tyrasamine；
Figure 10 is trans- cucurbit(7)uril iQ [7] and histamine1H NMR titration figure (500MHz, D2O)；
Figure 11 is the isothermal titration calorimetric figure of trans- cucurbit(7)uril iQ [7] and histamine；
Figure 12 is trans- cucurbit(7)uril iQ [7] and spermidine1H NMR titration figure (500MHz, D2O)；
Figure 13 is the isothermal titration calorimetric figure of trans- cucurbit(7)uril iQ [7] and spermidine.
Specific embodiment
Embodiment 1.Tryptamines, recognition methods are as follows for identification for a kind of trans- cucurbit(7)uril identification biogenic amine:
A. the trans- cucurbit(7)uril of 2mg is put into nuclear magnetic tube, the D of 0.6mL is added2O concussion, makes it dissolve, obtains A product；
B. 2mg biogenic amine to be detected is put in cryopreservation tube, the D of 1.0mL is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtains a series of nuclear-magnetism of host-guest complexes (obtain based on iQ [7], tryptamines as the host-guest complex of object)
Spectrogram, by it compared with tryptamines nuclear magnetic spectrogram, if occurring two groups of signal peaks Hg and Hf in map, wherein Hg is mobile toward low field,
Hf is mobile toward High-Field, while the signal peak peak type of other Ha~He proton broadens, and also mobile (such as Fig. 2 institute toward High-Field
Show), then illustrate that the biogenic amine is tryptamines.
Trans- cucurbit(7)uril identification biogenic amine above-mentioned is also used to identify spermine, and recognition methods is as follows:
A. the trans- cucurbit(7)uril of 2mg is put into nuclear magnetic tube, the D of 0.6mL is added2O concussion, makes it dissolve, obtains A product；
B. 2mg biogenic amine to be detected is put in cryopreservation tube, the D of 1.0mL is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtains a series of nuclear-magnetism of host-guest complexes (obtain based on iQ [7], spermine as the host-guest complex of object)
Spectrogram, by it compared with spermine nuclear magnetic spectrogram, if mobile, Hc is moved the signal peak-to-average power of Hd and He proton without obvious toward low field in map
Dynamic and Ha and Hb is mobile (as shown in Figure 6) toward High-Field, then illustrates that the biogenic amine is spermine.
Trans- cucurbit(7)uril identification biogenic amine above-mentioned is also used to identify spermidine, and recognition methods is as follows:
A. the trans- cucurbit(7)uril of 2mg is put into nuclear magnetic tube, the D of 0.6mL is added2O concussion, makes it dissolve, obtains A product；
B. the biogenic amine to be detected of 2mg is put in cryopreservation tube, the D of 1.0mL is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtains a series of core of host-guest complexes (obtain based on iQ [7], spermidine as the host-guest complex of object)
Magnetic spectrum figure, by it compared with spermidine nuclear magnetic spectrogram, if the signal peak of Hd proton is mobile toward low field in map, and Ha, Hb, Hc,
The signal peak-to-average power of He, Hf, Hg proton is mobile (as shown in figure 12) to High-Field in various degree, then illustrates that the biogenic amine is spermidine.
Trans- cucurbit(7)uril identification biogenic amine above-mentioned is also used to identify tyrasamine, and recognition methods is as follows:
A. the trans- cucurbit(7)uril of 2mg is put into nuclear magnetic tube, the D of 0.6mL is added2O concussion, makes it dissolve, obtains A product；
B. 2mg biogenic amine to be detected is put in cryopreservation tube, the D of 1.0mL is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtains a series of nuclear-magnetism of host-guest complexes (obtain based on iQ [7], tyrasamine as the host-guest complex of object)
Spectrogram, by it compared with tyrasamine nuclear magnetic spectrogram, if the signal peak-to-average power of the Hc and Hd proton in map on alkyl chain is mobile toward High-Field,
If the signal peak-to-average power of the proton in map on phenyl ring is mobile (as shown in Figure 8) toward High-Field, illustrates that the biogenic amine is tyrasamine.
Trans- cucurbit(7)uril identification biogenic amine above-mentioned is also used to identify histamine, and recognition methods is as follows:
A. the trans- cucurbit(7)uril of 2mg is put into nuclear magnetic tube, the D of 0.6mL is added2O concussion, makes it dissolve, obtains A product；
B. 2mg biogenic amine to be detected is put in cryopreservation tube, the D of 1.0mL is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtains a series of nuclear-magnetism of host-guest complexes (obtain based on iQ [7], histamine as the host-guest complex of object)
Spectrogram, by it compared with histamine nuclear magnetic spectrogram, if the signal peak of the Ha and Hb proton in map on imidazole ring has past High-Field mobile
Trend, the signal peak of Hc and Hd proton on alkyl chain it is mobile (as shown in Figure 10) toward High-Field and low field respectively, then illustrate this
Biogenic amine is histamine.
Trans- cucurbit(7)uril identification biogenic amine above-mentioned is also used to identify phenyl ethylamine, and recognition methods is as follows:
A. the trans- cucurbit(7)uril of 2mg is put into nuclear magnetic tube, the D of 0.6mL is added2O concussion, makes it dissolve, obtains A product；
B. 2mg biogenic amine to be detected is put in cryopreservation tube, the D of 1.0mL is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram, with the increasing of B product amount
Add, obtains a series of core of host-guest complexes (obtain based on iQ [7], phenyl ethylamine as the host-guest complex of object)
Magnetic spectrum figure, by it compared with phenyl ethylamine nuclear magnetic spectrogram, if the signal peak-to-average power of the Hd and He proton in map on alkyl chain is toward low field
The signal peak-to-average power of proton mobile, on phenyl ring is mobile (as shown in Figure 4) toward High-Field, then illustrates that the biogenic amine is phenyl ethylamine.
Claims (6)
1. a kind of application of trans- cucurbit(7)uril identification biogenic amine, which is characterized in that tryptamines for identification, recognition methods are as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram and obtains with the increase of B product amount
To a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with tryptamines nuclear magnetic spectrogram, if occurring two groups of signal peaks in map
Hg and Hf, wherein Hg is mobile toward low field, and Hf is mobile toward High-Field, while the signal peak peak type of other Ha~He proton becomes
Width, and it is also mobile toward High-Field, then illustrate that the biogenic amine is tryptamines.
2. the application of trans- cucurbit(7)uril identification biogenic amine according to claim 1, which is characterized in that be also used to identify essence
Amine, recognition methods are as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram and obtains with the increase of B product amount
To a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with spermine nuclear magnetic spectrogram, if in map Hd and He proton letter
Toward low field, mobile, Hc is mobile toward High-Field without apparent motion and Ha and Hb for number peak, then illustrates that the biogenic amine is spermine.
3. the application of trans- cucurbit(7)uril identification biogenic amine according to claim 1 or 2, which is characterized in that be also used to know
Other spermidine, recognition methods are as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram and obtains with the increase of B product amount
To a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with spermidine nuclear magnetic spectrogram, if in map Hd proton signal
Peak is mobile toward low field, and the signal peak-to-average power of Ha, Hb, Hc, He, Hf, Hg proton is mobile to High-Field, then illustrates the biogenic amine for sub- essence
Amine.
4. the application of trans- cucurbit(7)uril identification biogenic amine according to claim 1 or 2, which is characterized in that be also used to know
Other tyrasamine, recognition methods are as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram and obtains with the increase of B product amount
To a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with tyrasamine nuclear magnetic spectrogram, if Hc in map on alkyl chain and
The signal peak-to-average power of Hd proton is mobile toward High-Field, if the proton in map on phenyl ring signal peak-to-average power it is mobile toward High-Field, say
The bright biogenic amine is tyrasamine.
5. the application of trans- cucurbit(7)uril identification biogenic amine according to claim 1 or 2, which is characterized in that be also used to know
Other histamine, recognition methods are as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram and obtains with the increase of B product amount
To a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with histamine nuclear magnetic spectrogram, if Ha in map on imidazole ring and
The signal peak of Hb proton has the tendency that the signal peak of Hc and Hd proton mobile toward High-Field, on alkyl chain respectively toward High-Field and low field
It is mobile, then illustrate that the biogenic amine is histamine.
6. the application of trans- cucurbit(7)uril identification biogenic amine according to claim 1 or 2, which is characterized in that be also used to know
Other phenyl ethylamine, recognition methods are as follows:
A. trans- cucurbit(7)uril is put into nuclear magnetic tube, D is added2O concussion, makes it dissolve, obtains A product；
B. biogenic amine to be detected is put in cryopreservation tube, D is added2O makes it dissolve, and obtains B product；
C. B product are gradually added in A product, every dropwise addition once obtains a corresponding nuclear magnetic spectrogram and obtains with the increase of B product amount
To a series of nuclear magnetic spectrogram of host-guest complexes, by it compared with phenyl ethylamine nuclear magnetic spectrogram, if the Hd in map on alkyl chain
It is mobile toward High-Field with the signal peak-to-average power of the signal peak-to-average power proton mobile toward low field, on phenyl ring of He proton, then illustrate the biogenic amine
For phenyl ethylamine.
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