CN107058135A - A kind of bacterial strain for producing zytase and its application - Google Patents
A kind of bacterial strain for producing zytase and its application Download PDFInfo
- Publication number
- CN107058135A CN107058135A CN201710399095.2A CN201710399095A CN107058135A CN 107058135 A CN107058135 A CN 107058135A CN 201710399095 A CN201710399095 A CN 201710399095A CN 107058135 A CN107058135 A CN 107058135A
- Authority
- CN
- China
- Prior art keywords
- zytase
- active component
- production
- bacterial strain
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/885—Trichoderma
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
Abstract
The present invention relates to the bacterial strain of production zytase and its application, the production of zytase can effectively be solved, problem the need for meet industrially, method is, the inoculation for producing zytase is cultivated in enzymatic production culture medium, obtain the zymotic fluid of zytase, centrifugation, ammonium sulfate is slowly added dropwise in supernatant, saltout overnight, liquid of saltouing is centrifuged, take precipitation, precipitated with buffer solution, fill post, equilibrium liquid is used as using buffer solution, carry out DAE FF gel permeation chromatographies, collect active component, refill post, linear elution is carried out with level pad, collect active component, ammonium sulfate mass concentration in active component is adjusted to 40%, fill post, equilibrium liquid linear elution is used as using ammonium sulphate buffer, collect active component, dialysis desalination, freezing is drained, obtain dry powder zytase.Present invention production zytase is a kind of new bacterial strain tangerine green trichoderma（Trichoderma citrinoviride）HL28 5, has effects that to produce zytase, has opened up the new way of zytase production.
Description
Technical field
The present invention relates to biology enzyme, particularly a kind of bacterial strain for producing zytase and its application.
Background technology
Zytase（1,4-β-D-xylanase:EC3.2.1.8）It is a kind of important industrial enzymes, is decomposing xylan
When from biocatalysis, can be by xylanolitic into polysaccharide or the protein or RNA of monose.Zytase is widely present in
In the organism of nature.Zytase can be produced in bacterium, fungi, animal body etc..
Zytase includes 1.3-beta-xylanase of inscribe（EC3.2.1.32）, 1.4-beta-xylanase of inscribe
（EC3.2.1.8）, 1.3-xylobiase of xylan（EC3.2.1.72）, 1.4-xylobiase of xylan
（EC3.2.1.37）And acetyl xylan esterase（EC3.1.1.6）Five kinds.Internal cutting type xylanase hydrolyzed xylan generation wood is few
Sugar and xylose, circumscribed-type xylanase hydrolysis wood oligose, xylose residues are cut off by non-reducing end.Zytase (1,4- β-D-
xylanase ；EC 3.2.1.8) it is a kind of important industrial enzymes, in the fields such as paper industry, food, feed and the energy
With important application value.Zytase can improve the whiteness of paper in paper industry as association with pulp bleaching auxiliary agent
And intensity, reduce the practicality of traditional chlorinated thing bleaching agent；Zytase is added in animal feed, can be carried with degradation of xylan
Utilization rate of the high animal to feed；In food industry, zytase can be also used for clear juice beverage, improve bread
Degree.Xylo-oligosaccharide can also be obtained using zytase degradation of xylan, xylo-oligosaccharide can promote probiotics in human body large intestine
Propagation, improve function of intestinal canal.In nature, the enzyme activity characteristic of the zytase of separate sources has very big difference, there is each
From special optimal reaction pH and optimum temperature.But, most zytase optimal reaction pH values are narrower 4.0~6.0
Optimal reaction pH limit the application of zytase industrially.
The content of the invention
For above-mentioned situation, to overcome the defect of prior art, the purpose of the present invention is just to provide a kind of production zytase
Bacterial strain and its application, can effectively solve the production of zytase, with meet it is industrial the need for problem.
The technical scheme that the present invention is solved is that a kind of strain classification for producing zytase is named as tangerine green trichoderma
（Trichoderma citrinoviride）HL28-5, preserving number：CGMCC NO.11861, preservation date：December 11 in 2015
Day, depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address：Chaoyang District, Beijing City
The institute 3 of North Star West Road 1；The bacterial strain is efficiently applied to produce zytase, and its application process comprises the following steps：
（1）, prepare the zymotic fluid of zytase：
The inoculation for producing zytase is cultivated in enzymatic production culture medium, the zymotic fluid of zytase is obtained；
（2）, the zymotic fluid of zytase centrifuged, obtain first time supernatant；
（3）, into first time supernatant ammonium sulfate is slowly added dropwise, saltout overnight, must saltout liquid；
（4）, the liquid that will saltout centrifugation, take precipitation, precipitated with buffer solution, obtain zytase crude enzyme liquid；
（5）, DAE FF gel permeation chromatographies：Zytase crude enzyme liquid is filled into post, using buffer solution as equilibrium liquid, DAE FF are carried out
Gel permeation chromatography, collects active component, obtains first time active component；
（6）, CM.FF strong cation exchanges chromatography：First time active component is filled into post, using buffer solution as equilibrium liquid, with balance
Buffer solution carries out linear elution, collects active component, obtains second of active component；
（7）, Octyl FF hydrophobic interaction chromatographies：By the ammonium sulfate mass concentration in second of active component adjust to
40%, post is filled, using ammonium sulphate buffer as equilibrium liquid linear elution, active component is collected, obtains third time active component；
（8）, desalination that third time composition is dialysed, freezing drains, and obtains dry powder zytase.
Present invention production zytase is a kind of new bacterial strain tangerine green trichoderma（Trichoderma citrinoviride）
HL28-5, has effects that to produce zytase, effective for preparing zytase, has opened up the new way of zytase production
Footpath, can effectively meet it is industrial to zytase the need for, have huge economic and social benefit.
Brief description of the drawings
Fig. 1 is production technological process of the invention.
Fig. 2 for the Trichoderma citrinoviride HL28-5 that screen of the present invention and its 18S rRNA sequence most
The phyletic evolution tree graph that 10 close strains are built.
Trichoderma citrinoviride HL28-5 growths and producing enzyme diagram in Fig. 3 present invention.
The optimum temperature figure of the high yield zytase of Fig. 4 present invention.
The temperature stability figure of the high yield zytase of Fig. 5 present invention.
The most suitable action pH figure of high yield zytase of Fig. 6 present invention.
The high yield zytase pH stability diagrams of Fig. 7 present invention.
Embodiment
The embodiment of the present invention is elaborated below in conjunction with concrete condition.
The present invention is in specific implementation, a kind of bacterial strain for producing zytase, and its screening is：
（1）, enrichment culture：
0.1g rotten wood is taken, is added in 10mL sterilized waters and vibrates mixing, 1mL bacteria suspensions is then drawn and is linked into 5mL enrichment cultures
In base, 30 DEG C of water-bath shaken cultivation 48h；
Described enriched medium is that dusty yeast 1g, peptone 2g, xylan 2g and water 1L mixings are made；
（2）, transparent circle method primary dcreening operation：
The culture 10 of enrichment culture is diluted step by step again, it is 10 that extension rate is taken respectively-3、10-5With 10-7Bacteria suspension it is each
0.5mL is coated on plate screening culture medium, and 37 DEG C of culture 24h, the relatively large single bacterium colony of picking transparent circle is isolated and purified,
Access slant preservation culture medium and preserve original strain；
Described plate screening culture medium is xylan 10g, KNO3 1g、MgSO4 0.2g、NaCl 0.5g、K2HPO4 0.5g, fine jade
Fat 20g and water 1L is mixed and is made；
Described slant preservation culture medium is that potato 200g, sucrose 20g, agar 20g and water 1L mixings are made；
（3）, shake flask fermentation secondary screening：
In the basic culture mediums of original strain access 100mL that primary dcreening operation is chosen, every group of 3 repetitions, in 30 DEG C, 180r/min
Lower culture 2-3d, zymotic fluid centrifuging and taking supernatant, DNS measures zymotic fluid xylanase activity respectively；
Described basic culture medium is wheat bran 4.0g, peptone 0.5g, K2HPO40.5g and water 1L is mixed and is made；
（4）, strain idenfication：
According to its 18S rDNA sequence alignment, select it is most close 13 plants with its sequence known to strain, constructing system chadogram（Figure
2）, it is tangerine green trichoderma Trichoderma citrinoviride HL28- to identify the production xylanase activity power highest strain
5, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center（CGMCC NO.11861）, preservation date：
On December 11st, 2015, depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address：
The institute 3 of BeiChen West Road, Chaoyang District, BeiJing City 1；
The bacterial strain is efficiently applied to produce zytase, and its application process comprises the following steps：
（1）, prepare the zymotic fluid of zytase：
By quality 2% inoculum concentration by produce zytase inoculation in enzymatic production culture medium, 30 DEG C, 180rpm shaking flasks
48h is cultivated, the zymotic fluid of zytase is obtained；
Described enzymatic production culture medium is by weight meter：Wheat bran 10g, xylan 2g, peptone 6g, dusty yeast 1.5g,
(NH4)2SO41.5g, CaCl2 0.3g, MgSO4 0.3g, KH2PO40.3g and FeSO40.005g and water 1000mL mixes system
Into；
（2）, by 4 DEG C of the zymotic fluid of zytase, 8000rpm, centrifugation 10min, take supernatant；
（3）, ammonium sulfate is slowly added dropwise into supernatant, the mass content of ammonium sulfate is reached at 80%, 4 DEG C that saltout 8- overnight
12h, must saltout liquid；
（4）, will saltout at 4 DEG C of liquid, 8000rpm centrifugation 10min, take precipitation, it is heavy with pH7.0 Tris-HCl buffer solutions
Form sediment, obtain zytase crude enzyme liquid；
（5）, DAE FF gel permeation chromatographies：Zytase crude enzyme liquid is filled into post, column type 1ml, by pH8.0 0.02mol/L
Tris-HCl buffer solutions are used as equilibrium liquid, flow velocity：1mL/min carries out DAE FF gel permeation chromatographies, collects active component, obtains the
Active component；
（6）, CM.FF strong cation exchanges chromatography：First time active component is filled into post, column type 1ml, by pH8.0 0.02mol/L
Tris-HCl buffer solutions are used as equilibrium liquid, flow velocity：1mL/min, linear elution is carried out with 1.0M NaCl level pad, is received
Collect active component, obtain second of active component；
（7）, Octyl FF hydrophobic interaction chromatographies：By the ammonium sulfate mass concentration in second of active component adjust to
40%, post is filled, hydrophobic interaction chromatography, column type 1ml, pH8.0, the 0.02mol/L sulphur containing 40% saturation degree ammonium sulfate is carried out
Sour ammonium buffer solution is used as equilibrium liquid, flow velocity：1mL/min, with 1M ammonium sulfate linear elution, collects active component, obtains third time
Active component；
（8）, dialysis desalination：Third time composition is transferred in the bag filter equipped with 20 mmol/L Tris-HCl buffer solutions 1L,
Dialysed overnight desalination, bag filter molecular cut off is 3500 u, and then freezing is drained, and obtains dry powder zytase.
Bacterial strain of the present invention is a kind of new bacterial strain, the effect of with high yield zytase, effective for production zytase,
And extraordinary advantageous effects are achieved through experiment, relevant information is as follows：
The bacterial strain of described high yield zytase be it is isolated inside rotten wood, by investigate its morphological feature,
Physiological and biochemical property and the contrast of 18S rRNA sequence signatures, filter out with bacterial strain known to immediate 13 plants of its sequence, build system
System chadogram, as shown in Fig. 2 being accredited as tangerine green trichoderma（Trichoderma citrinoviride）, HL28-5 is named as, is protected
China Committee for Culture Collection of Microorganisms's common micro-organisms center is hidden in, preserving number is CGMCC NO.11861.It is prepared
The relative molecular weight of zytase be 34.8KDa, the optimum temperature of zytase is 50 DEG C, the most suitable work of zytase
Use pH7.0.
18S rRNA sequences：
gagaatccgg ggttcactcc aacccaatgt gaacgttacc aatctgttgc ctcggcggga 60
ttctctgccc cgggcgcgtc gcagccccgg atcccatggc gcccgccgga ggaccaactc 120
aaactctttt ttctctccgt cgcggcctac gtcgcggctc tgttttattt ttgctctgag 180
cctttctcgg cgaccctagc gggcgtctcg aaaatgaatc aaaactttca acaacggatc 240
tcttggttct ggcatcgatg aagaacgcag cgaaatgcga taagtaatgt gaattgcaga 300
attcagtgaa tcatcgaatc tttgaacgca cattgcgccc gccagtattc tggcgggcat 360
gcctgtccga gcgtcatttc aaccctcgaa cccctccggg gggtcggcgt tggggatcgg 420
cccctcaccg ggccgccccc gaaatacagt ggcggtctcg ccgcagcctc tcctgcgcag 480
tagtttgcac actcgcaccg ggagcgcggc gcggccacag ccgtaaaaca ccccaaactc 540
tgaaatgttg acctcggatc aggtaggaat acccgctgaa cttaagcata tcaaaaggcg 600
gaggaa 606
Experiment 1
Xylanase activity is determined：180 μ L contain the substrate of 1% xylan, add the enzyme liquid that 20 μ L suitably dilute, and 50 DEG C accurate anti-
Answer and 200 μ L 3,5- dinitrosalicylic acids are added after 10min（Dinitrosalicylic acid, DNS）Reagent solution terminates anti-
Should, boiling water boiling 5min colour developings are rapidly cooled to add 2.1mL water after room temperature, OD values are then determined at 540nm wavelength.According to
The regression equation calculation of xylose standard curve goes out the sugar content of reaction system.
It is to the definition of xylanase activity unit of force in experiment：At 50 DEG C, under the conditions of PH7.0, hydrolyzed xylan per minute
1 μm of ol reduced sugar of enzyme（In terms of xylose）Required enzyme amount is defined as 1 enzyme activity unit（1U）.
IU=D × R/10min × 0.1mL
In formula：D is enzyme liquid extension rate；R is the Xylose Content that regression equation calculation goes out.
Zytase is subjected to SDS-PAGE analyses, single band is obtained, and determine that it has xylan through enzyme spectrum analysis
Enzymatic activity, reaches that electrophoresis is pure.
Experiment 2：The zymologic property of zytase
The determination of the zytase relative molecular weight
It will ferment obtained crude enzyme liquid and zytase after purification carry out SDS-PAGE respectively, analysis obtains its relative molecular weight
About 34.8KDa.
Experiment 3：Influence of the temperature to enzyme activity
(1) respectively at 20 DEG C, 30 DEG C, 40 DEG C, 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C, this is determined under the conditions of pH7.0 wooden poly- after purification
The enzyme activity of carbohydrase, is repeated 3 times, and averages, and calculates the enzyme activity under different temperatures.
As a result as shown in figure 4, its optimum temperature is 50 DEG C.
(2) temperature stability of the zytase is determined：By this after purification zytase enzyme liquid be respectively placed in different temperatures
(30 DEG C, 40 DEG C, 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C), pH be 7.0 under conditions of, be incubated 2h, every 30min sample survey
Enzyme activity, calculates respective residual enzyme activity, the enzyme activity of initial enzyme liquid is set into 100%.As a result such as Fig. 5, less than 50 DEG C
In the range of, the temperature stability of the enzyme is all preferable, and it is little with respect to Xylanase activity change, and when temperature is more than 50 DEG C,
The temperature stability of the enzyme is deteriorated, and its enzyme activity declines rapidly.
Experiment 4：Influences of the pH to enzyme activity
(1), the measure of the most suitable action pH of the zytase：Different pH (3.0,4.0,5.0,6.0,7.0,8.0,9.0,
10.0th, 11.0,12.0), temperature is the enzyme activity that the zytase after purification is determined under conditions of 50 DEG C, using highest enzyme activity as
100%, calculate the enzyme activity under different pH.
As a result as shown in Fig. 6, its most suitable action pH is 7.0.
(2), the pH Stability Determinations of the zytase：By this after purification zytase enzyme liquid be adjusted to different pH respectively
Under the conditions of (3.0,4.0,5.0,6.0,7.0,8.0,9.0,10.0,11.0,12.0), temperature 50 C, after insulation 2h, determine each
From residual enzyme activity, wherein highest enzyme activity is set to 100%.As a result as shown in Fig. 7, the enzyme is steady in pH5.0-10.0
It is qualitative preferable.
From the above, it is seen that the invention provides the bacterial strain of production zytase, after strain idenfication, being named as the green wood of tangerine
Mould HL28-5, for producing zytase, and has carried out zymologic property experiment measure, average molecular to zytase after purification
Measure as 34.8KDa, optimum temperature and pH are respectively 50 DEG C and 7.0；The enzyme in the range of 30 DEG C~50 DEG C and pH5.0-11.0
Work keeps relative stability, and quality is good, can be effectively used for multiple industrial circles such as papermaking, food and feed, has a wide range of application, and is wood
Innovation in dextranase production, economic and social benefit is huge.
SEQUENCE LISTING
<110>Henan Academy of Sciences Biological Research Institute Co., Ltd.
<120>A kind of bacterial strain for producing zytase and its application
<130> 2017.5
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 606
<212> DNA
<400> 1
gagaatccgg ggttcactcc aacccaatgt gaacgttacc aatctgttgc ctcggcggga 60
ttctctgccc cgggcgcgtc gcagccccgg atcccatggc gcccgccgga ggaccaactc 120
aaactctttt ttctctccgt cgcggcctac gtcgcggctc tgttttattt ttgctctgag 180
cctttctcgg cgaccctagc gggcgtctcg aaaatgaatc aaaactttca acaacggatc 240
tcttggttct ggcatcgatg aagaacgcag cgaaatgcga taagtaatgt gaattgcaga 300
attcagtgaa tcatcgaatc tttgaacgca cattgcgccc gccagtattc tggcgggcat 360
gcctgtccga gcgtcatttc aaccctcgaa cccctccggg gggtcggcgt tggggatcgg 420
cccctcaccg ggccgccccc gaaatacagt ggcggtctcg ccgcagcctc tcctgcgcag 480
tagtttgcac actcgcaccg ggagcgcggc gcggccacag ccgtaaaaca ccccaaactc 540
tgaaatgttg acctcggatc aggtaggaat acccgctgaa cttaagcata tcaaaaggcg 600
gaggaa 606
Claims (3)
1. a kind of bacterial strain for producing zytase, Classification And Nomenclature is tangerine green trichoderma（Trichoderma citrinoviride）HL28-
5, China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved in, deposit number CGMCC NO.11861 are protected
Hide the date：On December 11st, 2015, depositary institution：China Committee for Culture Collection of Microorganisms's common micro-organisms center, protects
Hide address：The institute 3 of BeiChen West Road, Chaoyang District, BeiJing City 1.
2. application of the bacterial strain of the production zytase described in claim 1 in production zytase.
3. application process of the bacterial strain of the production zytase described in claim 2 in production zytase, it is characterised in that bag
Include following steps：
（1）, prepare the zymotic fluid of zytase：
By quality 2% inoculum concentration by produce zytase inoculation in enzymatic production culture medium, 30 DEG C, 180rpm shaking flasks
48h is cultivated, the zymotic fluid of zytase is obtained；
Described enzymatic production culture medium is by weight meter：Wheat bran 10g, xylan 2g, peptone 6g, dusty yeast 1.5g,
(NH4)2SO41.5g, CaCl2 0.3g, MgSO4 0.3g, KH2PO40.3g and FeSO40.005g and water 1000mL mixes system
Into；
（2）, by 4 DEG C of the zymotic fluid of zytase, 8000rpm, centrifugation 10min, take supernatant；
（3）, ammonium sulfate is slowly added dropwise into supernatant, the mass content of ammonium sulfate is reached at 80%, 4 DEG C that saltout 8- overnight
12h, must saltout liquid；
（4）, will saltout at 4 DEG C of liquid, 8000rpm centrifugation 10min, take precipitation, it is heavy with pH7.0 Tris-HCl buffer solutions
Form sediment, obtain zytase crude enzyme liquid；
（5）, DAE FF gel permeation chromatographies：Zytase crude enzyme liquid is filled into post, column type 1ml, by pH8.0 0.02mol/L
Tris-HCl buffer solutions are used as equilibrium liquid, flow velocity：1mL/min carries out DAE FF gel permeation chromatographies, collects active component, obtains the
Active component；
（6）, CM.FF strong cation exchanges chromatography：First time active component is filled into post, column type 1ml, by pH8.0 0.02mol/L
Tris-HCl buffer solutions are used as equilibrium liquid, flow velocity：1mL/min, linear elution is carried out with 1.0M NaCl level pad, is received
Collect active component, obtain second of active component；
（7）, Octyl FF hydrophobic interaction chromatographies：By the ammonium sulfate mass concentration in second of active component adjust to
40%, post is filled, hydrophobic interaction chromatography, column type 1ml, pH8.0, the 0.02mol/L sulphur containing 40% saturation degree ammonium sulfate is carried out
Sour ammonium buffer solution is used as equilibrium liquid, flow velocity：1mL/min, with 1M ammonium sulfate linear elution, collects active component, obtains third time
Active component；
（8）, dialysis desalination：Third time composition is transferred in the bag filter equipped with 20 mmol/L Tris-HCl buffer solutions 1L,
Dialysed overnight desalination, bag filter molecular cut off is 3500 u, and then freezing is drained, and obtains dry powder zytase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710399095.2A CN107058135A (en) | 2017-05-31 | 2017-05-31 | A kind of bacterial strain for producing zytase and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710399095.2A CN107058135A (en) | 2017-05-31 | 2017-05-31 | A kind of bacterial strain for producing zytase and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107058135A true CN107058135A (en) | 2017-08-18 |
Family
ID=59616254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710399095.2A Pending CN107058135A (en) | 2017-05-31 | 2017-05-31 | A kind of bacterial strain for producing zytase and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107058135A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108004186A (en) * | 2018-01-09 | 2018-05-08 | 北京工商大学 | A kind of bacterial strain and application produced hydrolyzable and prepare high polymerization degree XOS zytases |
| CN113564056A (en) * | 2021-08-13 | 2021-10-29 | 南京林业大学 | High-yield xylanase strain Fusicolla sp7-2 and application thereof |
| CN114574372A (en) * | 2022-03-28 | 2022-06-03 | 山东省林业科学研究院 | Trichoderma citrinoviride and application thereof in degrading fish protein |
-
2017
- 2017-05-31 CN CN201710399095.2A patent/CN107058135A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 马焕等: "产木聚糖酶菌株的筛选、鉴定及其酶学性质研究", 《中国酿造》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108004186A (en) * | 2018-01-09 | 2018-05-08 | 北京工商大学 | A kind of bacterial strain and application produced hydrolyzable and prepare high polymerization degree XOS zytases |
| CN113564056A (en) * | 2021-08-13 | 2021-10-29 | 南京林业大学 | High-yield xylanase strain Fusicolla sp7-2 and application thereof |
| CN114574372A (en) * | 2022-03-28 | 2022-06-03 | 山东省林业科学研究院 | Trichoderma citrinoviride and application thereof in degrading fish protein |
| CN114574372B (en) * | 2022-03-28 | 2023-06-20 | 山东省林业科学研究院 | Trichoderma citrinoviride and application thereof in degradation of fish protein |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Inbar et al. | Evidence that chitinase produced by Aeromonas caviae is involved in the biological control of soil-borne plant pathogens by this bacterium | |
| Chivero et al. | Partial purification and characterisation of a xylanase enzyme produced by a micro-organism isolated from selected indigenous fruits of Zimbabwe | |
| Thrane et al. | Substrate colonization, strain competition, enzyme production in vitro, and biocontrol of Pythium ultimum by Trichoderma spp. isolates P1 and T3 | |
| CN108929859A (en) | One type bacterial strain of bacillus HB172198 and its application | |
| CN107058135A (en) | A kind of bacterial strain for producing zytase and its application | |
| KR101207584B1 (en) | Strain of sanguibacter keddieii having an alginate-decomposition activity and method of producing alginate-oligomer using the same | |
| CN110257410A (en) | A kind of gene encoding algin catenase | |
| Guilloux-Benatier et al. | Lysis of yeast cells by Oenococcus oeni enzymes | |
| CN108018275A (en) | A kind of mutant XYNR of extremely thermostable xylanase 1VBR and application thereof | |
| CN102191203A (en) | Bacillus amyloliquefaciens and method for producing chymosin by fermenting using same | |
| CN104031860B (en) | A kind of spherical bacillus of high-yield thermostable zytase and application thereof | |
| Han et al. | Optimization of cold-active chitinase production from the Antarctic bacterium, Sanguibacter antarcticus KOPRI 21702 | |
| CN104130950A (en) | Aspergillus niger and cultivation method and application thereof | |
| CN102321558B (en) | High-yield strain of high temperature resistant 1,4-beta-D-xylanase, method for producing high temperature resistant 1,4-beta-D-xylanase through fermentation of high-yield strain, and high temperature resistant 1,4-beta-D-xylanase | |
| CN106754486B (en) | Pseudomonas for high-yield trehalose synthase and fermentation enzyme production method thereof | |
| CN101415722A (en) | A method for the preparation of K-carrageenase | |
| CN107739721A (en) | Produce bacillus sp.W2017 and its application of agar-agar digestive enzyme | |
| CN103031289A (en) | Lactase and recombinant expression engineering bacterium thereof | |
| CN110093387A (en) | A method of chitosan oligosaccharide is prepared using chitin in bacillus and Paecilomyces varioti Synergistic degradation shrimp and crab shells | |
| CN109810918B (en) | Bacillus atrophaeus with effect of preventing wolfberry leaf blight, biological agent and application of biological agent | |
| CN108728370A (en) | The salmon subfamily Renibacterium bacterial strain QD-01 and its fermentation process of one plant height effect production chitosan enzyme and application | |
| Cadirci et al. | Comparison of in vitro antifungal activity methods using extract of chitinase-producing Aeromonas sp. BHC02 | |
| Abdulameer et al. | Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation | |
| Aly et al. | Molecular characterization of chitiniolytic Bacillus pumilus isolated from marine habitats and enhancement of chitinase production by mutation | |
| Kumar et al. | Purification, characterization and gene encoding of xylanase produced from Bacillus tequilensis SH0 isolated from compost using low cost wheat bran as substrate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170818 |