CN107055800A - A kind of analogy method of calamus to lead accumulation ability in water - Google Patents
A kind of analogy method of calamus to lead accumulation ability in water Download PDFInfo
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- CN107055800A CN107055800A CN201710313430.2A CN201710313430A CN107055800A CN 107055800 A CN107055800 A CN 107055800A CN 201710313430 A CN201710313430 A CN 201710313430A CN 107055800 A CN107055800 A CN 107055800A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/3103—Atomic absorption analysis
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
- C02F2101/22—Chromium or chromium compounds, e.g. chromates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/3103—Atomic absorption analysis
- G01N2021/3114—Multi-element AAS arrangements
Abstract
The invention belongs to heavy metal pollution of water body technical field, a kind of analogy method of calamus to lead accumulation ability in water is disclosed, including：Vial 25 of the product of trying to please for 1L, chooses each vial after the same plant trimming of growing way and puts three plants of calamus, by vial with 1 25 serial numbers；The method that outdoor culture is used to the growth management of plant, every 7 days to nutrient solution in water-soluble lead concentration and zinc concentration detected with flame atomic absorption method, while by the waste liquid in vial added to mark；With quartering precise sample aerial part and under ground portion；The solution for having suspension is poured into the small fire heating on electric hot plate in beaker, it is repeated multiple times until ash content is completely dissolved.The present invention by simulated experiment, calamus in different pollution environment to water in lead accumulation ability, the application for being calamus in water pollution improvement and environment remediation provides reference；Growth of the leaded waste liquid of low concentration to plant plays a driving role.
Description
Technical field
The invention belongs to heavy metal pollution of water body technical field, more particularly to a kind of calamus is to the mould of lead accumulation ability in water
Plan method.
Background technology
As social economy is fast-developing, all kinds of synthetic materials, medicine and chemical products are widely used, and environmental pollution is asked
Topic becomes increasingly conspicuous.In environmental contaminants, heavy metal toxicity is big, it is difficult to eliminate, and is enriched with amplification by food chain, endangers people
Class health.Wherein, lead is one of maximum heavy metal contaminants of known toxicity, with extremely strong cumulative bad and irreversibility.Water
Lead in body is mainly derived from the discharge of rock, soil, atmospheric falling dust and lead waste water, and geological conditions and leaded gold
The water of the conveying of metal conduit will all cause the pollution of lead, and its available sources is numerous, be common heavy metal contaminants in water environment
One of.Heavy metal pollution of water body has two kinds of basic governance ways：One is the migration and conversion energy for reducing heavy metal in water body
Power is to fix, and two be that heavy metal is thoroughly removed in water body.At present, it is used for fixing or removing heavy metal reparation water environment both at home and abroad
Method mainly have peripheral doses, chemical remediation and biological restoration.It is biological prosthetic to have compared to physics and chemical remediation
Cost saving, non-secondary pollution, the features such as environmental risk is small, as emerging study hotspot, phytoremediation is biological prosthetic one
Individual branch.Nineteen eighty-three, American scientist Chaney proposes to eliminate the imagination of heavy metal pollution using plant first, by section
Educational circles and government department's accreditation and selection.The heavy metal contaminants in water environment, and tool can be fixed and absorbed to water plant
There is improvement aquatic ecological environment there is provided viewing and admiring and economic value, widely should be obtained in water pollution improvement and environment remediation
With.Select suitable plant variety to be the key of phytoremediation the absorption enriched character of pollutant according to plant, therefore surround
Comparative study of the different wetland plants to a certain Metal uptake accumulation ability is more.But it is often multiple pollutant in water environment
Coexist, absorption of the plant to certain heavy metal species is enriched with not only by plant itself affect, and pollutant also is coexisted by other in environment
Influence.Pollutant in environment is varied, and their influences to plants enriched heavy metal ability are had nothing in common with each other.Current experiment
Scheme is often used under same pollutional condition, different wetland plant heavy metal accumulation ability contrasts, lacks different dirts
The simulation of the contrast of the accumulation ability of wetland plant and the relevant influence of combined pollution under the conditions of dye.
In summary, the problem of prior art is present be：Calamus is indefinite to the accumulation ability of lead in water, it is impossible to which reflection is multiple
Close influence of the pollution to calamus accumulation ability.
The content of the invention
The problem of existing for prior art, the invention provides a kind of simulation side of calamus to lead accumulation ability in water
Method.
The present invention is achieved in that a kind of analogy method of calamus to lead accumulation ability in water, and the calamus is in water
The analogy method of lead accumulation ability comprises the following steps：
Step one, vial 25 of the product of trying to please for 1L, chooses each vial after the same plant trimming of growing way and puts three
Strain calamus, by vial with 1-25 serial numbers；
Step 2, the method for using outdoor culture to the growth management of plant, remains sufficient illumination and ventilation, often
Every 7 days to nutrient solution in water-soluble lead concentration and zinc concentration detected with flame atomic absorption method, while by vial
Waste liquid is added to mark；
Step 3, with quartering precise sample aerial part and under ground portion, is put into 5mL crucibles, first in low temperature
Carbonized powder 30min on electric furnace；Crucible is transferred in Muffle furnace again and is ashed 5h；1 is added into the sample powder after ashing:1
Salpeter solution 10mL simultaneously instills 1 drop hydrogen peroxide, carries out the dilution of sample, extracts, filters afterwards in 25mL volumetric flasks, and spend
Ionized water is settled to scale；
Step 4, the small fire heating on electric hot plate in beaker is poured into by the solution for having suspension, repeated multiple times until ash content
It is completely dissolved, cooled and filtered enters in 25mL volumetric flasks, and scale is settled to deionized water, whole process, which is used, adds 10mL deionizations
Water and add 1 drip hydrogen peroxide as control, finally to measure Pb with flame atomic absorption method2+And Zn2+Content.
Further, in the step one：Wherein No. 1-5 be simulating pollution thing with Pb (NO3)2Form addition, use deionization
Water dissolving Pb (NO3)2And it is configured to Pb2+Concentration gradient is the common design of 0mg/L, 5mg/L, 10mg/L, 20mg/L, 50mg/L experiment
5 kinds of processing；
Without Pb2+Nutrient solution be control group, No. 6-10 be (Pb (NO3)2+Zn(NO3) 2) combined pollution, Pb2+Concentration
It is fixed as 10mg/L, Zn2+With Zn (NO3)2Form addition, Zn2+Concentration gradient be 0mg/L, 5mg/L, 10mg/L, 20mg/L,
50mg/L experiments set 5 groups of processing altogether, without Zn2+Containing Pb2+10mg/L nutrient solution is control group.
No. 11-15 is (Pb (NO3)2+ EDTA) complexing pollution, Pb2+Concentration is fixed as 10mg/L, and EDETATE DISODIUM adds dense
Degree is respectively that 0mg/L, 5mg/L, 10mg/L, 20mg/L, 50mg/L experiment set 5 groups of processing altogether, contains Pb without EDTA2+10mg/L
Nutrient solution be control group.
No. 16-20 is (Pb (NO3)2+Zn(NO3)2) combined pollution, Pb2+Concentration is fixed as 50mg/L, Zn2+Add concentration point
Not Wei 0mg/L, 5mg/L, 10mg/L, 50mg/L, 100mg/L experiment set 5 groups of processing altogether, without Zn2+Containing Pb2+50mg/L's
Nutrient solution is control group；
21-25(Pb(NO3)2+ EDTA) complexing pollution, Pb2+With EDETATE SODIUM with 1:1 mass concentration is added, concentration point
Not Wei 0mg/L, 5mg/L, 10mg/L, 50mg/L, 100mg/L experiment set 5 groups of processing altogether, without Pb2+With EDTA nutrient solution
For control group.
Further, in the step 2：Sample plant after processing is rinsed well with running water, the water on plant is wiped
It is dry, it is divided into aerial part and under ground portion electronic balance scale claims its fresh weight respectively；The 1h that finishes is put into baking oven, after fixing
Plant sample dries 7h to constant weight at 80 DEG C, claims its weight by the sample electronic balance of drying, obtains aerial part and ground
The dry weight of lower part；The sample of constant weight is ground with mortar, and sample powder is put into standby in vinyl bag.
Another object of the present invention is to provide a kind of application calamus to the analogy method of lead accumulation ability in water
Water body lead contamination processing system.
Another object of the present invention is to provide a kind of application calamus to the analogy method of lead accumulation ability in water
Heavy metal pollution of water body processing system.
Advantages of the present invention and good effect are：Chang is studied by simulating various concentrations lead waste water and combined pollution waste water
Pu is to lead accumulation ability in water；Calamus is analyzed to the enrichment condition of the lead of various concentrations, the accumulation ability of calamus plant root, stem and leaf
And when Zn, EDTA and Pb coexist calamus to lead enrichment condition.The present invention can be the place of phytoremediation heavy metal-polluted water
Reason technology is referred to, by simulated experiment obtain calamus in different pollution environment to water in lead accumulation ability, be calamus
Application in water pollution improvement and environment remediation provides reference.Experiment shows that growth of the leaded waste liquid of low concentration to plant rises
Facilitation；Lead acts on the root of plant first when entering plant, heavy metal effectively can not be transferred to cauline leaf by calamus
Part, causes root substantially to be poisoned；Zn presence promotes absorption of the plant to Pb, the performance of both toxic actions to plant
For cooperative effect；And in the leaded waste liquid containing EDTA, the ability of plant absorption lead is suppressed, the poison of Pb and EDTA to plant
Property effect show as antagonistic effect.Above simulated experiment is carried out on a small scale in the lab, and with low cost, experiment condition is controllable.
Brief description of the drawings
Fig. 1 is analogy method flow chart of the calamus provided in an embodiment of the present invention to lead accumulation ability in water.
Fig. 2 is calamus plant biomass variety schematic diagram provided in an embodiment of the present invention.
Fig. 3 is the long change schematic diagram of calamus plant root provided in an embodiment of the present invention.
Fig. 4 is calamus plant height change schematic diagram provided in an embodiment of the present invention.
Fig. 5 is provided in an embodiment of the present invention in Pb (NO3)2While various concentrations in the waste liquid that initial concentration is 10mg/L
Zn(NO3)2In the presence of Pb2+Change in concentration schematic diagram.
Fig. 6 is provided in an embodiment of the present invention in Pb (NO3)2While various concentrations in the waste liquid that initial concentration is 10mg/L
Pb in the presence of EDTA2+Change in concentration schematic diagram.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle of the present invention is explained in detail below in conjunction with the accompanying drawings.
As shown in figure 1, calamus provided in an embodiment of the present invention includes following step to the analogy method of lead accumulation ability in water
Suddenly：
S101：Vial 25 of the product of trying to please for 1L, chooses each vial after the same plant trimming of growing way and puts three plants
Calamus, by vial with 1-25 serial numbers, wherein No. 1-5 is simulating pollution thing with Pb (NO3)2Form addition, use deionization
Water dissolving Pb (NO3)2And it is configured to Pb2+Concentration gradient is the common design of 0mg/L, 5mg/L, 10mg/L, 20mg/L, 50mg/L experiment
5 kinds of processing；
S102：The method for using outdoor culture to the growth management of plant, remains sufficient illumination and ventilation, every
Water-soluble lead concentration and zinc concentration in nutrient solution were detected with flame atomic absorption method in 7 days, while will be useless in vial
Liquid is added to mark；
S103：With quartering precise sample aerial part and under ground portion (0.4000 ± 0.0001) g, 5mL is put into
In crucible, first carbonized powder (about 30min) untill not smoldering on low-temperature furnace；Crucible is transferred in Muffle furnace again and is ashed
5h；1 is added into the sample powder after ashing:1 salpeter solution 10mL simultaneously instills 1 drop hydrogen peroxide, carries out the dilution of sample, carries
Take, filter afterwards in 25mL volumetric flasks, and scale is settled to deionized water；
S104：The solution for having suspension is poured into the small fire heating on electric hot plate in beaker, it is repeated multiple times until ash content is complete
Fully dissolved, cooled and filtered enters in 25mL volumetric flasks, and is settled to scale with deionized water, and whole process, which is used, adds 10mL deionized waters
And add 1 drip hydrogen peroxide as control, finally to measure Pb with flame atomic absorption method2+And Zn2+Content.
The application effect of the present invention is explained in detail with reference to experiment.
1 materials and methods
1.1 test material
Test plant is Acorus (Acorus L.) plant calamus (Acorus calamus L.).Living plant is cultivated in four
The water in Chuan Sheng Chengdu new capital county nursery plants seedling, and acquisition time is on May 4th, 2016.It is before experiment that root is clear with running water
Wash clean is placed in Nature enemy one week in the running water after being exposed to the sun.The nutrient solution containing 5mL and tool concentration gradient are put into afterwards
Waste liquid in cultivate.
1.2 test method
Vial 25 of the product of trying to please for 1L, chooses each vial after the same plant trimming of growing way and puts three plants of calamus,
By vial with 1-25 serial numbers, wherein No. 1-5 is simulating pollution thing with Pb (NO3)2Form addition, it is molten with deionized water
Solve Pb (NO3)2And it is configured to Pb2+Concentration gradient is that 0mg/L, 5mg/L, 10mg/L, 20mg/L, 50mg/L test 5 kinds of design altogether
Processing, wherein without Pb2+Nutrient solution be control group.No. 6-10 is (Pb (NO3)2+Zn(NO3)2) combined pollution, Pb2+Concentration
It is fixed as 10mg/L, Zn2+With Zn (NO3)2Form addition, Zn2+Concentration gradient be 0mg/L, 5mg/L, 10mg/L, 20mg/L,
50mg/L experiments set 5 groups of processing altogether, without Zn2+Containing Pb2+10mg/L nutrient solution is control group.No. 11-15 is (Pb (NO3)2
+ EDTA) complexing pollution, Pb2+Concentration is fixed as 10mg/L, and it is respectively 0mg/L, 5mg/L, 10mg/ that EDETATE DISODIUM, which adds concentration,
L, 20mg/L, 50mg/L experiment set 5 groups of processing altogether, contain Pb without EDTA2+10mg/L nutrient solution is control group.No. 16-20
It is (Pb (NO3)2+Zn(NO3)2) combined pollution, Pb2+Concentration is fixed as 50mg/L, Zn2+It is respectively 0mg/L, 5mg/ to add concentration
L, 10mg/L, 50mg/L, 100mg/L experiment set 5 groups of processing altogether, without Zn2+Containing Pb2+50mg/L nutrient solution is control group.
21-25(Pb(NO3)2+ EDTA) complexing pollution, Pb2+With EDETATE SODIUM with 1:1 mass concentration is added, and concentration is respectively 0mg/
L, 5mg/L, 10mg/L, 50mg/L, 100mg/L experiment set 5 groups of processing altogether, without Pb2+Nutrient solution with EDTA is control group.
The method for using outdoor culture to the growth management of plant, remains sufficient illumination and ventilation.It is right every 7 days
Water-soluble lead concentration and zinc concentration are detected with flame atomic absorption method in nutrient solution, while the waste liquid in vial is added
To mark.Plant is observed during this period, recorded, indoor temperature change generated in case scope is 20-25 DEG C.By the sample after processing
Plant is rinsed well with running water, and the water on plant is dried, and is divided into aerial part and under ground portion is distinguished with electronic balance scale
Claim its fresh weight.The 1h that finishes is put into baking oven, the plant sample after fixing 7h is dried into constant weight at 80 DEG C, by the sample of drying
Claim its weight with electronic balance, obtain the dry weight of aerial part and under ground portion.The sample of constant weight is ground with mortar, and by sample
Product powder is put into standby in vinyl bag.
1.3 assay method
With quartering precise sample aerial part and under ground portion (0.4000 ± 0.0001) g, 5mL crucibles are put into
In, first carbonized powder (about 30min) untill not smoldering on low-temperature furnace.Crucible is transferred in Muffle furnace again and is ashed 5h；To
1 is added in sample powder after ashing:1 salpeter solution 10mL simultaneously instills 1 drop hydrogen peroxide, carries out the dilution of sample, extracts, it
After filter in 25mL volumetric flasks, and be settled to scale with deionized water, the solution for having suspension poured into beaker in electric heating
Small fire is heated on plate, and repeated multiple times until ash content is completely dissolved, cooled and filtered enters in 25mL volumetric flasks, and fixed with deionized water
Hold to scale, whole process add 10mL deionized waters and the drop hydrogen peroxide of addition 1 as control, finally to use Flame Atomic Absorption Spectrometry
Method measures Pb2+And Zn2+Content.
2 results and analysis
2.1 growth response
2.1.1 the victimization state of plant different tissues
Calamus purifies contaminated leaded water body by absorbing, being enriched with the lead in water, but the lead worked as in water body reaches
Certain concentration then produces a certain degree of damage to the different tissues of plant.
(1) calamus is placed in the lead waste water of various concentrations gradient and cultivated, slightly turned to be yellow at blade edge at the 3rd day
Sign, all turned to be yellow in the 10th day blade.The reason for yellowing leaf is probably that its leaf is influenceed after the murder by poisoning of lead because plant receives
Green element synthesis is reduced or degraded increases, and changes the ratio of carotenoid in plants and chlorophyll, so that blade is due to a lack of leaf
It is green element and yellow.
(2) situation that root is done harm to by lead poisoning, when cultivating the 7th day, root begins with sprouting and grown, when cultivating the 30th day
Root starts to decay.
2.1.2 plant growth situation
During calamus is cultivated, the height of the biomass of calamus plant, the root length of plant and plant occurs
Change.As shown in Fig. 2 when lead concentration is less than 10mg/L, biomass increased；When concentration is 20mg/L, under biomass
Drop, it is seen that the leaded waste liquid of low concentration plays a driving role to the growth metabolism of calamus.As knowable to Fig. 3 and Fig. 4, the plant done harm to by lead poisoning
Root length and plant height growth change it is less obvious, and the root length of the plant of non-leading waste liquid increases very fast, illustrates leaded waste liquid
Growth to the root of plant has played certain inhibitory action.
2.2 combined pollutions are studied
2.2.1Pb, Zn combined pollutions are studied
Calamus plant is cultivated in the waste water containing Pb, Zn waste liquid, the partial blade of plant starts jaundice, the 7th at the 3rd day
It when plant blade all turn to be yellow.As shown in figure 5, the concentration of the Pb in water sample after cultivating 35 days is almost 0, it can be seen that
Promote absorption of the plant to lead in the presence of Zn.It can be seen that, cooperative effect is shown as to the murder by poisoning of plant in Zn and Pb, Zn's deposits
Making, the poisoning of plant is even more serious.
2.2.2Pb, EDTA combined pollutions are studied
Calamus plant is cultivated in the waste water containing Pb, EDTA waste liquid, the blade edge of plant slightly turns to be yellow at the 3rd day,
Partial blade turns to be yellow at the 10th day.As shown in fig. 6, as the concentration of EDTA in water sample increases, the concentration of lead increases in water sample, can
See that EDTA presence suppresses absorption of the plant to lead.Murders by poisoning of the EDTA and Pb to plant shows as antagonistic effect, EDTA presence
Mitigate toxic actions of the Pb to plant.
Enrichment condition of the different tissues of 2.3 plants to lead
The enriching quantity of plant increases with the increase of lead concentration it can be seen from Data Data in table 1, and both are in positive
Pass relation.The concentration coefficient of plant root is far longer than the concentration coefficient of cauline leaf, illustrates that lead is mainly acted on after entering plant
The root of plant.So as to understand that concentration coefficient is bigger, its accumulation ability is stronger, and enriching quantity is bigger.It is both less than again by transfer ratio
1, between 0.05~0.30, illustrate that the heavy metal of root effectively can not be transferred to cauline leaf part by calamus plant.
Enriching quantity=plant heavy metal uptake/plant dry weight (mg/kg)
Concentration coefficient=plant shoot (or root) content of beary metal/solution constituent content
Transfer ratio=aboveground vegetation part content of beary metal/root the constituent content
Enriching quantity, concentration coefficient and transfer ratio of the calamus of table 1 to the Pb of various concentrations
Calamus is different to the absorption strength of lead in the leaded waste liquid of various concentrations, and calamus same concentrations Zn-Pb,
It is different to the absorbability of lead in Pb-EDTA combined pollution waste liquids.When lead concentration is less than 10mg/L, biomass increased；
When lead concentration is 20mg/L, biomass declines；The root length and change of height of the plant done harm to by lead poisoning is not obvious, and non-leading is useless
The root length of the plant of liquid increases very fast.In waste liquid at the same time containing Pb and Zn, Pb's is dense in the water sample after 35 days cultivate
Degree is almost 0mg/L.And in the waste liquid at the same time containing Pb and EDTA, increase with EDTA concentration in water sample, Pb's is dense in water sample
Degree is higher.For the enriching quantity of plant, the enriching quantity of plant increases with the increase of lead concentration, the concentration coefficient of root
It is far longer than the concentration coefficient of cauline leaf.
It can obtain in summary, facilitation is generally played in growth of the leaded waste liquid of low concentration to plant, but to plant roots
Growth have certain inhibitory action.Lead acts on the root of plant first when entering plant, but calamus can not effectively by
Heavy metal is transferred to cauline leaf part, causes root substantially to be poisoned.Zn promotes absorption of the plant to Pb, both poison to plant
Property effect show as cooperative effect.And in the leaded waste liquid containing EDTA, the ability of plant absorption lead is suppressed, Pb and EDTA
Antagonistic effect is shown as to the toxic action of plant.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (5)
1. a kind of calamus is to the analogy method of lead accumulation ability in water, it is characterised in that the calamus is to lead accumulation ability in water
Analogy method comprise the following steps：
Step one, vial 25 of the product of trying to please for 1L, chooses each vial after the same plant trimming of growing way and puts three plants of Chang
Pu, by vial with 1-25 serial numbers；
Step 2, the method for using outdoor culture to the growth management of plant, remains sufficient illumination and ventilation, every 7
It detects to water-soluble lead concentration and zinc concentration in nutrient solution with flame atomic absorption method, while by the waste liquid in vial
Added to mark；
Step 3, with quartering precise sample aerial part and under ground portion, is put into 5mL crucibles, first in low-temperature furnace
Upper carbonized powder 30min；Crucible is transferred in Muffle furnace again and is ashed 5h；1 is added into the sample powder after ashing:1 nitric acid
Solution 10mL simultaneously instills 1 drop hydrogen peroxide, carries out the dilution of sample, extracts, filters afterwards in 25mL volumetric flasks, and use deionization
Water is settled to scale；
Step 4, the small fire heating on electric hot plate in beaker is poured into by the solution for having suspension, repeated multiple times until ash content is complete
Dissolving, cooled and filtered enters in 25mL volumetric flasks, and is settled to scale with deionized water, and whole process, which is used, adds 10mL deionized waters simultaneously
Add 1 drip hydrogen peroxide as control, finally to measure Pb with flame atomic absorption method2+And Zn2+Content.
2. calamus as claimed in claim 1 is to the analogy method of lead accumulation ability in water, it is characterised in that the step one
In：Wherein No. 1-5 be simulating pollution thing with Pb (NO3)2Form addition, with deionized water dissolving Pb (NO3)2And it is configured to Pb2+
Concentration gradient is that 0mg/L, 5mg/L, 10mg/L, 20mg/L, 50mg/L test 5 kinds of processing of design altogether；
Without Pb2+Nutrient solution be control group, No. 6-10 be (Pb (NO3)2+Zn(NO3)2) combined pollution, Pb2+Concentration is fixed
For 10mg/L, Zn2+With Zn (NO3)2Form addition, Zn2+Concentration gradient is 0mg/L, 5mg/L, 10mg/L, 20mg/L, 50mg/
L experiments set 5 groups of processing altogether, without Zn2+Containing Pb2+10mg/L nutrient solution is control group；
No. 11-15 is (Pb (NO3)2+ EDTA) complexing pollution, Pb2+Concentration is fixed as 10mg/L, and EDETATE DISODIUM adds concentration point
Not Wei 0mg/L, 5mg/L, 10mg/L, 20mg/L, 50mg/L experiment set 5 groups of processing altogether, without EDTA contain Pb2+10mg/L battalion
Nutrient solution is control group；
No. 16-20 is (Pb (NO3)2+Zn(NO3)2) combined pollution, Pb2+Concentration is fixed as 50mg/L, Zn2+Adding concentration is respectively
0mg/L, 5mg/L, 10mg/L, 50mg/L, 100mg/L experiment set 5 groups of processing altogether, without Zn2+Containing Pb2+50mg/L nutrition
Liquid is control group；
21-25(Pb(NO3)2+ EDTA) complexing pollution, Pb2+With EDETATE SODIUM with 1:1 mass concentration is added, and concentration is respectively
0mg/L, 5mg/L, 10mg/L, 50mg/L, 100mg/L experiment set 5 groups of processing altogether, without Pb2+With EDTA nutrient solution for pair
According to group.
3. calamus as claimed in claim 1 is to the analogy method of lead accumulation ability in water, it is characterised in that the step 2
In：Sample plant after processing is rinsed well with running water, the water on plant is dried, be divided into aerial part and under ground portion is used
Electronic balance scale claims its fresh weight respectively；The 1h that finishes is put into baking oven, the plant sample after fixing is dried into 7h to perseverance at 80 DEG C
Weight, claims its weight by the sample electronic balance of drying, obtains the dry weight of aerial part and under ground portion；With mortar by constant weight
Sample is ground, and sample powder is put into standby in vinyl bag.
4. water body lead of the calamus to the analogy method of lead accumulation ability in water described in a kind of application claims 1 to 3 any one
Pollution treatment system.
5. water body weight of the calamus to the analogy method of lead accumulation ability in water described in a kind of application claims 1 to 3 any one
Metallic pollution processing system.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110389115A (en) * | 2018-04-18 | 2019-10-29 | 天津师范大学 | Utilize the method and its application of panorama cell multidimentional system detection water body cadmium ion |
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