CN107055764A - The simple measuring device and assay method of a kind of sludge activity and its application in Sewage Biological Treatment - Google Patents
The simple measuring device and assay method of a kind of sludge activity and its application in Sewage Biological Treatment Download PDFInfo
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- CN107055764A CN107055764A CN201710174335.9A CN201710174335A CN107055764A CN 107055764 A CN107055764 A CN 107055764A CN 201710174335 A CN201710174335 A CN 201710174335A CN 107055764 A CN107055764 A CN 107055764A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/30—Nature of the water, waste water, sewage or sludge to be treated from the textile industry
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
Application the present invention relates to sludge activity detection technique and its in Sewage Biological Treatment.The invention discloses a kind of simple measuring device of sludge activity in sewage biological treatment system and the assay method of sludge activity.For the consuming of on-line checking sludge activity greatly, the features such as easy break-down, a set of off-line detection device is devised.One of features of the present invention is not use traditional aeration oxygen supply, but uses hydrogen peroxide to decompose the mode for producing oxygen for microorganism oxygen supply.The two of feature are to being corrected in sludge activity calculation formula.Apparatus of the present invention are simple in construction, are easy to carry.And favorable reproducibility, it is stable, enrich the computational methods of sludge activity.
Description
Technical field
The present invention relates to a kind of measure device and method of sludge activity, sludge in more particularly to a kind of Sewage Biological Treatment
The simple measuring device and method of activity, belongs to technical field of sewage.
Background technology
Traditional method is activated sludge process in Sewage Biological Treatment, and wherein sludge activity refers to that biological components living are accounted for always
The ratio of biological components.
Active sludge oxygen-consuming rate (OUR) is defined as in activated Sludge System, microorganism unit interval when being breathed
The amount of interior consumption oxygen.Maximum OUR (OURmax) then be OUR maximum.The OUR of activated sludge is to characterize
One of important parameter of sludge activity, physiological status and the matrix generation of activated sludge are reflected from the respiratory rate angle of microorganism
Thank to situation.Initial stage is applied in activated sludge process, the respiratory rate of aerobic microbiological is just used to investigate microbial biomass and nutrients
Utilization rate.OUR in analysis, is evaluated and forecasting system operation conditions and processing as the parameter of detection sludge activity in recent years
By the most attention of domestic and international expert in ability.Research and the OUR detection techniques of application sewage biological treatment system, and then
To sludge activity parameter, the fortune of sewage biological treatment system is strengthened and improved in the development for promoting dirty water living creature processing technique
Row is of great significance with control, tool.
Nowadays, it is to determine OUR according to the change of system dissolved oxygen that sludge activity, which determines the method generally used,.According to
Whether in real time monitoring can be divided into batch (-type) and continous way again.
Intermittent operation is placed in mud mixed liquid to be measured in completely enclosed closed container, and is first aerated to dissolved oxygen
Concentration is close to saturation, then stops the dissolved oxygen concentration change in aeration, dissolved oxygen instrument record closed container.Slope of a curve is then
OUR.Although the method can not be monitored in real time, for the sewage disposal system of some shortage real-time watch devices,
Also support can be provided for the permanently effective operation of system.
And continous way operation then dissolved oxygen concentration at import and export in continuous alternately monitoring aeration tank.According to dissolved oxygen concentration
Difference and residence Time Calculation go out OUR.With automatic control equipment, on-line checking instrument and converter technique exploitation with
Using they are to realize that on-line checking sludge activity creates condition.
At present, biomass is generally suspended admittedly by determining mud mixed liquid in sewage treatment area, activated Sludge System
Bulk concentration (MLSS) and VSS concentration (MLVSS) are represented.However, except active microorganism in the two indexs
Amount is outer, in addition to the organic and inorganic component equal size in microbial metabolic products, water inlet.Therefore, table is carried out using these indexs
Error when sludge activity is calculated can be greatly increased by showing during active biomass in activated sludge, eventually result in and sludge activity is detected
It is inaccurate.
The content of the invention
One of the object of the invention is in view of the shortcomings of the prior art on the basis of repetition test, establishes a set of offline inspection
Survey the simple measuring device for reflecting sludge activity simultaneously.By investigating activated sludge OUR changing rule, lived to calculate sludge
Property.
The second object of the present invention is there is provided the simple measuring side of sludge activity for live the deficiencies in the prior art
Method.Although there is hysteresis quality, technical support can be provided.The technical solution adopted in the present invention is as follows：
A kind of simple measuring device of sludge activity, it includes constant temperature blender with magnetic force, constant temperature pond, reaction bulb, magnetic force
Stirrer, rubber stopper, adding of agent pipe, dissolved oxygen instrument and probe, constant temperature pond are placed in constant temperature blender with magnetic force, reaction bulb position
In constant temperature pond, magnetic stir bar is located at reaction bulb bottom, and rubber stopper covers reaction bulb bottleneck, adding of agent pipe, dissolved oxygen
The probe of instrument passes through in rubber stopper intercalation reaction bottle.
During start-up operation, mixed liquor DO (dissolved oxygen) is added in reaction bulb 3, covers and is inserted with dissolved oxygen instrument probe and medicament
The rubber stopper of pipe is added, starts constant temperature blender with magnetic force, magnetic stir bar work, dissolved oxygen instrument start recording mixed liquor DO becomes
Change, automatic one numerical value of record per minute, draw DO-t curves, the slope of a curve is shown as the OUR of active microorganism.
The effect of constant temperature blender with magnetic force constant temperature blender with magnetic force is to keep mixed liquor sample equal in suspending when OUR is determined
One state so that measurement result is stable, reliable.
It is preferred that, constant temperature blender with magnetic force uses heat collecting type constant temperature blender with magnetic force.
The method that the present invention also provides the measure sludge activity using above-mentioned simple measuring device
The reaction unit that the present embodiment is used includes preaeration system, reaction system, monitoring system, MLSS, MLVSS survey
Determine system.
First in small-sized aerator, pass through the aeration fully degraded wherein available nutrients of microorganism.
Then MLSS and MLVSS is determined using national standard method.
When OUR, which determines device, starts working, add what nitrification inhibitor, microorganism needed into reaction system
Trace element, nutritive salt, phosphate buffer, add hydrogenperoxide steam generator and provide the oxygen that microbial respiratory needs, finally add
Enter the carbon source (COD) of microbial respiratory needs, dissolved oxygen instrument record dissolved oxygen content per minute, until close to zero.Draw out
Dissolved oxygen versus time curve, selects greatest gradient, obtains OURmax, sludge activity is calculated further according to formula.
Comprise the following steps that：
(1) each 500ml of mud mixed liquid of aeration tank front end, middle part and rear end is taken, mixes, is placed in 1L aerator
In, using aeration oxygen supply aeration 20-30h, microorganism is made full use of nutriment.
(2) the above-mentioned mud mixed liquids of about 200-300ml are taken in 300ml reaction bulbs.Reaction bulb is placed in band constant temperature magnetic force and stirred
Mix in the constant temperature pond on device, temperature is 20-40 DEG C, be now not turned on constant temperature blender with magnetic force, bottleneck is opened wide.
(3) allylthiourea 0.3ml, liquid microelement I, liquid microelement II and nutrient solution are sequentially added into reaction bulb
Each 1ml, phosphate buffer 2ml.
Allylthiourea is concentration 10.0g/L Allyl thiourea solution；
Liquid microelement I be containing cobalt, iron, manganese, boron, zinc, molybdenum, nickel, copper solution；Wherein cobalt element content is
0.01-0.08g/L；Iron content is 1-2g/L；Manganese element content is 0.01-0.08g/L；Boron content is 0.001-
0.008g/L；Zn-ef ficiency content is 0.01-0.1g/L；Molybdenum element content is 0.01-0.08g/L；Nickel element content is 0.001-
0.008g/L；Copper coin cellulose content is 0.001-0.008g/L；
Liquid microelement II is calcic, the solution of magnesium elements；Wherein calcium element content is 2-6g/L；Magnesium element content is 2-
6g/L；
The nutrient solution is the nutrient solution containing nitrogen phosphate and sulfur；Nitrogen element content is 2-20g/L；Phosphorus element content is 2-20g/
L；Sulfur content is 2-20g/L；
Phosphate buffer is that phosphorus element content is 10-60g/L.
(4) 3~4 3% hydrogenperoxide steam generators of drop are slowly added into reaction bulb again, close plug opens agitating device, and rotating speed is
250rpm, the time is 30s.Then 1min is stood, hydrogen peroxide can decompose generation oxygen in the case of -20-30 DEG C, stirring.
Whether detection DO reaches 15mg/L.Otherwise, hydrogen peroxide is continuously added.
DO is reached after 15mg/L, adds 1ml sodium acetate solutions, opens agitating device, the time is 30s, is stood 1min, is added
Mud mixed liquid is full of reaction bulb.The probe of dissolved oxygen instrument is inserted, close plug is again turned on constant temperature blender with magnetic force, per 1min
Record dissolved oxygen changes with time, until close to zero；.
The sodium acetate solution is：Sodium acetate concentration is 160g/L, the COD equivalent to 100000mg/L；
(5) dissolved oxygen versus time curve DO-t is drawn out, the greatest gradient of trade-off curve is OURmax。
(6) mud mixed liquid suspended sediment concentration MLSS and VSS concentration are determined according to National Standard Method
MLVSS,.
(7) formula calculates sludge activity：Active%=6.4 × OURmax/Xvss。
The present invention is corrected in the calculation formula of sludge activity, have modified the computational methods of the amount of active microorganism.
Concentration (the X of active microorganism in sludgea) can be according to the dynamic characteristic meter that nutrients is utilized under dynamics saturation state
Calculate, i.e., at S (nutrient concentrations)>>KSIn the case of (saturation constant), sludge microbe is not dense by nutrients using nutrition
Degree influence, but the activity limitation of receptor 1 activity microorganism, microbial activity are higher, and the dissolved oxygen content of degradation of organic substances consumption is got over
Greatly, the maximum OUR that can be reached is higher.
In formula:
OURmax- maximum OUR；
β-often produces 1mgVSS oxygen demand, mg O2/ mg VSS, typically take 1.42；
Yg- Biomass yield coefficient, mg VSS/mg COD, typically takes 0.5；
Qm-maximum COD removal rates, mg COD/mg Xa/ h, typically takes 0.5；
ba- attenuation coefficient, d-1, typically take 0.2.(foundation of general value is sewage treatment plant's technological design handbook, high
Person of outstanding talent's hair is write)
At 25 DEG C, the calculation formula of sludge activity can be reduced to:
Application of the sludge activity easy device of the present invention in dyeing and printing sewage processing, measures sludge activity and is not less than 0.25
(mgO2/ gram MLVSS. hours) when, system operation is good.
Beneficial effects of the present invention are as follows：
1st, the simple measuring apparatus structure of sludge activity is simple, be readily disassembled, small volume is easy to carry, and cost of manufacture
It is low.
2nd, the present invention is without the traditional aeration oxygen supply of use, but uses hydrogen peroxide to decompose the mode for producing oxygen to be micro-
Biological oxygen supply.Hydrogen peroxide, which is decomposed, only produces water and oxygen, and environment is safe from harm.Compared to aeration oxygen supply, oxygen directly with point
Sub- form is transmitted to cell, will not form gas-liquid mass transfer resistance, improves the mass transfer rate of oxygen.Dissolved oxygen content can reach
15mg/L, oxygen supply is aerated better than tradition.Dissolved oxygen content height can guarantee that the normal metabolic activity of microorganism, can also extend sludge work
Property determine time, further ensure data accuracy.
Brief description of the drawings
Fig. 1 sludge activities of the present invention determine the structural representation of device
Dissolved oxygen changes with time in Fig. 2 OUR detection process
Sludge activity changes in Fig. 3 Zhejiang printing and dyeing company aeration tank mud mixed liquid
Sludge activity changes before and after Fig. 4 Zhejiang pad dyeing company toxic wastewater flows into aeration tank
Brief description of the drawings：1：Constant temperature blender with magnetic force；2：Constant temperature pond；3：Reaction bulb；4：Magnetic stir bar；5：Dissolved oxygen instrument
Probe；6：Rubber stopper；7：Adding of agent pipe；8：Dissolved oxygen instrument
Embodiment
The simple measuring device of sludge activity as shown in Figure 1, including constant temperature blender with magnetic force 1, constant temperature pond 2, reaction
Bottle 3, magnetic stir bar 4, rubber stopper 6, adding of agent pipe 7, dissolved oxygen instrument and probe 5.
Constant temperature pond 2 is placed in 1 in constant temperature blender with magnetic force, and reaction bulb 3 is located in constant temperature pond 2, and magnetic stir bar 4 is located at
The bottom of reaction bulb 3, rubber stopper 6 covers the bottleneck of reaction bulb 3, and adding of agent pipe 7, the probe 5 of dissolved oxygen instrument are inserted by rubber stopper 6
In reaction bulb 3.
During start-up operation, mixed liquor DO is added in reaction bulb 3, probe 5 and adding of agent with dissolved oxygen instrument is covered
The rubber stopper 6 of pipe 7, starts constant temperature blender with magnetic force 1, and magnetic stir bar 4 works, and the start recording mixed liquor DO of dissolved oxygen instrument 8 becomes
Change, automatic one numerical value of record per minute, draw DO-t curves, the slope of a curve is shown as the OUR of active microorganism.
The effect of constant temperature blender with magnetic force is to keep mixed liquor sample to be in suspension uniform state when OUR is determined so that surveyed
Determine result stable, reliable.
For ease of understanding sludge activity portable measuring method for measuring of the present invention, it is as follows that the present invention enumerates embodiment.
Embodiment 1
Zhejiang printing and dyeing Co., Ltd is applied to using apparatus of the present invention, monthly the sludge activity in aeration tank is surveyed
It is fixed.
With in January, 2014 data instance, step is as follows：
(1) each 500ml of mud mixed liquid of aeration tank front end, middle part and rear end is taken, mixes, is placed in 1L aerator
In, air aeration 24h makes microorganism make full use of nutriment.
(2) the above-mentioned mud mixed liquids of about 280ml are taken in 300ml reaction bulbs 3.Reaction bulb 3 is placed in constant temperature blender with magnetic force 1
On constant temperature pond 2 in, temperature control is 25 DEG C, is now not turned on constant temperature blender with magnetic force 1, and bottleneck is opened wide.
(3) allylthiourea 0.3ml is once added into reaction bulb 3, for suppressing nitration reaction.It is liquid microelement I, micro-
There is provided nutrient necessary to microbial respiratory by secondary element liquid II and each 1ml of nutrient solution.Phosphate buffer solution 2ml, is used for
Maintain acid-base balance.
Allylthiourea is：Concentration 10.0g/L Allyl thiourea solution；
Liquid microelement I be containing cobalt, iron, manganese, boron, zinc, molybdenum, nickel, copper solution；Wherein cobalt element content is
0.062g/L；Iron content is 1.036g/L；Manganese element content is 0.0139g/L；Boron content is 0.0044g/L；Zinc
Constituent content is 0.0121g/L；Molybdenum element content is 0.0020g/L；Nickel element content is 0.0063g/L；Copper coin cellulose content is
0.0025g/L；
Liquid microelement II is calcic, the solution of magnesium elements；Wherein calcium element content is 5.4g/L；Magnesium element content is
2.36g/L；
Nutrient solution is the nutrient solution containing nitrogen phosphate and sulfur；Nitrogen element content is 13.9g/L；Phosphorus element content is 11.5g/L；Sulphur
Constituent content is 6.67g/L；
Phosphate buffer is：Phosphorus element content is 47g/L；
(4) 3~4 3% hydrogenperoxide steam generators of drop are slowly added into reaction bulb 3 again, rubber stopper 6 is covered, opens constant temperature magnetic
Power agitator 1, magnetic stir bar 4 works, and rotating speed is 250rpm, and the time is 30s.Then stand 1min, hydrogen peroxide at 25 DEG C,
Generation oxygen can be decomposed in the case of stirring.Whether detection DO reaches 15mg/L.Otherwise, hydrogen peroxide is continuously added.
DO reaches after 15mg/L that addition 1ml sodium acetate solutions are there is provided the carbon source that microorganism needs, now COD concentration
333mg/L.Constant temperature blender with magnetic force 1 is opened, magnetic stir bar 4 works, and the time is 30s, stand 1min, add mud mixed liquid
It is full of reaction bulb.The probe 5 of dissolved oxygen instrument is inserted, close plug is again turned on agitating device, constant temperature blender with magnetic force 1, per 1min
Record dissolved oxygen.
The sodium acetate solution is：Sodium acetate concentration is 160g/L, the COD equivalent to 100000mg/L；
Draw dissolved oxygen content to change over time, as shown in Figure 2.
(5) greatest gradient of calculated curve, obtains OURmax, by calculating, OURmaxFor 41.9mg/ (L.h).
(6) MLSS of mud mixed liquid is determined for 1020mg/L according to National Standard Method, MLVSS is 867mg/L.
(7) it is 0.31 further to calculate activity.
The continuous detection of activity is carried out to the printing and dyeing company aeration tank mud mixed liquid using the method in the present invention, figure is obtained
3, provide data supporting for the permanently effective operation of system.As in January, 2014 in July, 2014 aeration tank sludge activity is respectively
0.31、0.27、0.29、0.26、0.28、0.29、0.26.It is normal value or so, illustrates that system operation is good.Experiment was demonstrate,proved in the past
In bright normal aerating system, sludge activity is 0.25 or so.If being much higher than normal value, often point out sludge loading too high,
At this moment effluent quality is poor, and the organic matter of residual is more.If active be less than normal value for a long time, now sludge loading is low, in water outlet
Residual organic matter negligible amounts, processing is complete.If longtime running, also sludge can be made to solve wadding due to a lack of nutrition.
In the present embodiment, constant temperature blender with magnetic force uses heat collecting type constant temperature blender with magnetic force.
Embodiment 2
ShaoXing,ZheJiang pad dyeing Co., Ltd is applied to using apparatus of the present invention, reclaimed water regulating reservoir waste water is probed into and enters aerobic
After system, if produce impact to sewage disposal system.After reclaimed water regulating reservoir waste water enters aerobic system, continuous one week daily
Determine sludge activity.By taking determination of activity on July 20 as an example, comprise the following steps that：
(1) the sewerage aeration tank front end, each 500ml of the mud mixture at middle part and rear end are taken, mixes, is placed in 1L's
In aerator, air aeration 24h makes microorganism make full use of nutriment.
(2) the above-mentioned mud mixtures of about 280ml are taken in 300ml reaction bulbs 3.Reaction bulb is placed in band constant temperature blender with magnetic force
In constant temperature pond 2 on 1, temperature control is 25 DEG C, is now not turned on constant temperature blender with magnetic force 1, and bottleneck is opened wide.
(3) allylthiourea 0.3ml is once added into reaction bulb 3, for suppressing nitration reaction.It is liquid microelement I, micro-
There is provided nutrient necessary to microbial respiratory by secondary element liquid II and each 1ml of nutrient solution.Phosphate buffer solution 2ml, is used for
Maintain acid-base balance.
Allylthiourea is：Concentration 10.0g/L Allyl thiourea solution；
Liquid microelement I be containing cobalt, iron, manganese, boron, zinc, molybdenum, nickel, copper solution；Wherein cobalt element content is
0.062g/L；Iron content is 1.036g/L；Manganese element content is 0.0139g/L；Boron content is 0.0044g/L；Zinc
Constituent content is 0.0121g/L；Molybdenum element content is 0.0020g/L；Nickel element content is 0.0063g/L；Copper coin cellulose content is
0.0025g/L；
Liquid microelement II is calcic, the solution of magnesium elements；Wherein calcium element content is 5.4g/L；Magnesium element content is
2.36g/L；
Nutrient solution is the nutrient solution containing nitrogen phosphate and sulfur；Nitrogen element content is 13.9g/L；Phosphorus element content is 11.5g/L；Sulphur
Constituent content is 6.67g/L；
Phosphate buffer is：Phosphorus element content is 47g/L；
(4) 3~4 3% hydrogenperoxide steam generators of drop are slowly added into reaction bulb 3 again, rubber stopper 6 is covered, opens constant temperature magnetic
Power agitator 1, rotating speed is 250rpm, and the time is 30s, and magnetic stir bar 4 works.Then stand 1min, hydrogen peroxide at 25 DEG C,
Generation oxygen can be decomposed in the case of stirring.Whether detection DO reaches 15mg/L.Otherwise, hydrogen peroxide is continuously added.
DO is reached after 15mg/L, adds 1ml sodium acetate solutions there is provided the carbon source that microorganism needs, and is opened constant temperature magnetic force and is stirred
Device 1 is mixed, the time is 30s, stand 1min, adding mud mixed liquid is full of reaction bulb.The probe 5 of dissolved oxygen instrument 8 is inserted, it is close
Plug, is again turned on agitating device, and dissolved oxygen is recorded per 30s.Dissolved oxygen content is drawn to change over time.The sodium acetate solution
For：Sodium acetate concentration is 160g/L, the COD equivalent to 100000mg/L；
(5) greatest gradient of calculated curve, obtains OURmax, by calculating, OURmaxFor 73.1mg/ (L.h).
(6) MLSS of mud mixed liquid is determined for 2753mg/L according to National Standard Method, MLVSS is 1918mg/L.
(7) it is 0.26 further to calculate activity.
According to the step, the following activity measured for several days is followed successively by 0.18,0.19,0.18,0.20,0.22,0.23,
0.25、0.24、0.25。
Activity data change is as shown in Figure 4.(on July 21st, 2014 extra reclaimed water regulating reservoir waste water start to be discharged into it is aerobic
System)
Waste water is introduced into before aeration tank as can be seen from Figure 4, and sludge activity is 0.26.Into after aerobic system, activity
0.18 is reduced to, but the later stage gradually rises so that stable again.Microorganism is inadaptable to such waste water when this explanation starts, and causes activity
Reduction.But the influence of this impact and little, microorganism will not be made to be poisoned to death, microorganism just adapted to this water at second day
Matter, activity gradually recovers normal level.This result provides good for the scene operation of the pad dyeing Co., Ltd sewage disposal
Technical support.
The embodiment is used only for help and understands the present invention, is not construed as the concrete restriction to the present invention.
Unless specific instructions, various raw materials of the invention can be by being commercially available；Or according to the routine side of this area
Method is prepared.Unless otherwise defined or described herein, all specialties used herein are ripe with art technology with scientific words
Practice meaning known to the person of entering identical.In addition any method similar or impartial to described content and material all can be applied to this
In inventive method.Unless otherwise indicated, all specialties used herein and scientific words and those skilled in the art
Known meaning is identical.
Claims (4)
1. a kind of simple measuring device of sludge activity, it is characterised in that it includes constant temperature blender with magnetic force, constant temperature pond, anti-
Bottle, magnetic stir bar, rubber stopper, adding of agent pipe, dissolved oxygen instrument and probe are answered, the constant temperature pond is placed in temperature constant magnetic stirring
On device, reaction bulb is located in constant temperature pond, and magnetic stir bar is located at reaction bulb bottom, and rubber stopper covers reaction bulb bottleneck, medicament
Add pipe, the probe of dissolved oxygen instrument passes through in rubber stopper intercalation reaction bottle.
2. the method that simple measuring device described in a kind of usage right requirement 1 determines sludge activity, it is characterised in that including following
Step：
Step 1: taking each 500ml of mud mixed liquid of aeration tank front end, middle part and rear end, mix, be placed in 2L aerator
In, using aeration oxygen supply, 20-30h is aerated, microorganism is made full use of nutriment；
Step 2: taking the above-mentioned mud mixed liquids of about 200-300ml in the reaction bulb of simple measuring device, reaction bulb is placed in constant temperature
In in constant temperature pond on magnetic stirring apparatus, temperature is 20-40 DEG C, is now not turned on constant temperature blender with magnetic force, and bottleneck is opened wide；
Step 3: sequentially adding allylthiourea 0.3ml, liquid microelement I, liquid microelement II, nutrient solution into reaction bulb
Each 1ml, phosphate buffer 2ml；
The allylthiourea is concentration 10.0g/L Allyl thiourea solution；
The liquid microelement I be containing cobalt, iron, manganese, boron, zinc, molybdenum, nickel, copper solution；Wherein cobalt element content is
0.01-0.08g/L；Iron content is 1-2g/L；Manganese element content is 0.01-0.08g/L；Boron content is 0.001-
0.008g/L；Zn-ef ficiency content is 0.01-0.1g/L；Molybdenum element content is 0.01-0.08g/L；Nickel element content is 0.001-
0.008g/L；Copper coin cellulose content is 0.001-0.008g/L；
The liquid microelement II is calcic, the solution of magnesium elements；Wherein calcium element content is 2-6g/L；Magnesium element content is 2-
6g/L；
The nutrient solution is the nutrient solution containing nitrogen phosphate and sulfur；Nitrogen element content is 2-20g/L；Phosphorus element content is 2-20g/L；Sulphur
Constituent content is 2-20g/L；
The phosphate buffer is：Phosphorus element content is 10-60g/L；
Step 4: being slowly added to 3~4 drop 1-3% hydrogenperoxide steam generators into reaction bulb, close plug opens constant temperature blender with magnetic force,
Magnetic stir bar 4 works, and rotating speed is 250rpm, and the time is 30s, then stands 1min；Hydrogen peroxide at 20-30 DEG C, stirring
In the case of can decompose generation oxygen, detection DO (dissolved oxygen) whether reach 15mg/L, otherwise, continuously add hydrogen peroxide；Work as DO
Reach after 15mg/L, add 1-2ml sodium acetate solutions, open agitating device, the time is 30s, stand 1min, add sludge mixing
Liquid is full of reaction bulb；The probe of dissolved oxygen instrument is inserted, close plug is again turned on constant temperature blender with magnetic force, dissolving is recorded per 1min
Oxygen changes with time, until close to zero；
The sodium acetate solution is：Sodium acetate concentration is 160g/L, the COD equivalent to 100000mg/L；
Step 5: drawing out dissolved oxygen versus time curve DO-t, the greatest gradient of trade-off curve is OURmax, according to state
Mark method determines mud mixed liquid suspended sediment concentration MLSS and VSS concentration MLVSS, then calculates sludge with formula
Activity：
3. application of the sludge activity easy device described in claim 1 in dyeing and printing sewage processing, it is characterised in that measure sludge
Activity is not less than 0.25mgO2/ gram MLVSS. hours when, system operation is good.
4. simple measuring device according to claim 1, it is characterised in that the constant temperature blender with magnetic force is that heat collecting type is permanent
Warm magnetic stirring apparatus.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106086157A (en) * | 2016-06-21 | 2016-11-09 | 俞率成 | Catalase activity analyzer and using method thereof |
| CN114047228A (en) * | 2021-11-09 | 2022-02-15 | 同济大学 | Device and method for analyzing sediment oxygen-consuming pollutants |
| CN114609356A (en) * | 2022-04-11 | 2022-06-10 | 中山大学 | Accurate determination device and method for oxygen consumption rate of water body |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106010950A (en) * | 2016-06-27 | 2016-10-12 | 郭洪伟 | Method and device for simple determination of activity of nitrifying bacteria |
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- 2017-03-22 CN CN201710174335.9A patent/CN107055764A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106010950A (en) * | 2016-06-27 | 2016-10-12 | 郭洪伟 | Method and device for simple determination of activity of nitrifying bacteria |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106086157A (en) * | 2016-06-21 | 2016-11-09 | 俞率成 | Catalase activity analyzer and using method thereof |
| CN114047228A (en) * | 2021-11-09 | 2022-02-15 | 同济大学 | Device and method for analyzing sediment oxygen-consuming pollutants |
| CN114047228B (en) * | 2021-11-09 | 2023-08-04 | 同济大学 | Device and method for analyzing oxygen-consuming pollutants of sediment |
| CN114609356A (en) * | 2022-04-11 | 2022-06-10 | 中山大学 | Accurate determination device and method for oxygen consumption rate of water body |
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