CN107049952A - A kind of preparation method of tea polyphenol liposomes - Google Patents
A kind of preparation method of tea polyphenol liposomes Download PDFInfo
- Publication number
- CN107049952A CN107049952A CN201710398032.5A CN201710398032A CN107049952A CN 107049952 A CN107049952 A CN 107049952A CN 201710398032 A CN201710398032 A CN 201710398032A CN 107049952 A CN107049952 A CN 107049952A
- Authority
- CN
- China
- Prior art keywords
- tea polyphenols
- phosphate buffer
- buffer solution
- liposome
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
- A61K9/1277—Processes for preparing; Proliposomes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
- A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
Abstract
The present invention relates to a kind of preparation method of tea polyphenol liposomes, methods described is comprised the following steps：（1）Appropriate phosphatide and cholesterol are weighed, 30ml chloroforms is added and is allowed to dissolve；（2）Configure the phosphate buffer solution containing Tea Polyphenols；3）Cushioning liquid containing Tea Polyphenols is added in container, emulsified（1st time）Form W/O Reversed micelles；4）By Reversed micelles chloroform removed under pressure, add the cushioning liquid containing Tea Polyphenols and emulsified（2nd time）, w/o type emulsion is formed, decompression boils off organic solvent, produces liposome.The method of the invention prepares liposome using emulsion dispersion method, then free Tea Polyphenols is centrifuged, and has preferable encapsulating effect, is a kind of high preparation method of envelop rate, and the liposome particle size prepared is evenly distributed.
Description
Technical field
The invention belongs to the medicinal application field of Tea Polyphenols, and in particular to a kind of preparation method of tea polyphenol liposomes.
Background technology
Liposome has obtained rapid development as a kind of new drug carrier in the research of field of medicaments.Liposome is that have
The bimolecular vesica that phospholipid molecule is constituted, liposome has many advantages, such as pharmaceutical carrier：As liposome can encapsulate liposoluble
Property medicine, water soluble drug can be encapsulated again, wrapped medicine can be effectively protected, bioavilability is improved, medicine is had eight
Item effect, can reduce the toxic side effect of medicine, liposome has slow-releasing and controlled-releasing action, be adapted to multipath administration etc..Liposome due to
Its unique design feature, makes pharmaceutical preparation enter the new world of target administration, in medicine as a kind of new drug carrier
Boundary has obtained increasingly extensive concern.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of preparation method of tea polyphenol liposomes, and this method is easy,
Envelop rate is high, and liposomal particle size is uniform, and short preparation period, cost is low, suitable for scale production.
The technical scheme that the present invention is provided is：A kind of preparation method of tea polyphenol liposomes, it is characterised in that including as follows
Step：
（1）Appropriate 100 milligrams of soybean lecithin, 20-30 milligrams of cholesterol are weighed, 30ml chloroforms is added and is allowed to dissolve, mixed
Close solution；
（2）Configure the phosphate buffer solution containing Tea Polyphenols：The phosphate buffer solution that configuration pH value is 7.2-7.5 first, it is dense
Spend for 10-20 mMs/milliliter；The ratio in 10 milliliters of phosphate buffer solutions is added to according to 50-100 milligrams of Tea Polyphenols,
The phosphate buffer solution containing Tea Polyphenols is made；
（3）Take step（2）Obtained 10 milliliters of the phosphate buffer solution containing Tea Polyphenols is added to step（1）Obtained mixing is molten
In liquid, the 1st emulsification is carried out, W/O Reversed micelles are formed；
（4）By step（3）Middle W/O Reversed micelles chloroform removed under pressure, adds 10 milliliters of steps（2）What is obtained contains Tea Polyphenols
Phosphate buffer solution carry out the 2nd time emulsification, formed w/o type emulsion；Decompression boils off organic solvent, produces liposome.
The step of the present invention（1）In, phosphatide and cholesterol are added, phosphatide is the main chemical compositions for constituting liposome, is
One amphoteric ionic surfactant；Cholesterol is the main constituents of liposome membrane, plays a part of stabilized liposome.
The step of the present invention（1）Described in the phosphate buffer solution containing Tea Polyphenols belong to a kind of additives, Ke Yiti
The stability of high liposome or the targeting for improving liposome.
The invention has the advantages that：The present invention is using Tea Polyphenols as raw material, using the preparation of emulsion dispersion method, lipid
Body；Obtained tea polyphenol liposomes can produce the orientation of medicine, relatively more several treatments for acting on diseased region, improving medicine
Effect, reduces toxic side effect；Skin can be promoted in terms of cosmetics to embedding the absorption of composition and institute can be slowly released
The skin active material of embedding, increase active material contrast rich action time, additionally, due to composition liposome bimolecular film
Main component is phosphatide, and because the special class eucaryotic cell structure of liposome is allowed to than other materials（Including free phosphatide）More
It can be distributed in the readily permeable cuticula to skin and well there, due to the package action of liposome, can prevent from being permitted
The degraded of many active materials is with going bad.The inventive method is easy, and short preparation period, cost is low, meets environmental protection and the requirement of safety,
It is suitable for scale production.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.Phosphatide used and courage of the invention
Sterol is purchased from Shanghai Bai Ao bio tech ltd.
Embodiment 1
A kind of preparation method of tea polyphenol liposomes, comprises the following steps：
（1）Appropriate 100 milligrams of soybean lecithin, 25 milligrams of cholesterol are weighed, 30ml chloroforms is added and is allowed to dissolve, obtain mixing molten
Liquid；
（2）Configure the phosphate buffer solution containing Tea Polyphenols：The phosphate buffer solution that configuration pH value is 7.2-7.5 first, it is dense
Spend for 15 mMs/milliliter；The ratio in 10 milliliters of phosphate buffer solutions is added to according to 100 milligrams of Tea Polyphenols, is made and contains
The phosphate buffer solution of Tea Polyphenols；
（3）Take step（2）Obtained 10 milliliters of the phosphate buffer solution containing Tea Polyphenols is added to step（1）Obtained mixing is molten
In liquid, the 1st emulsification is carried out, W/O Reversed micelles are formed；
（4）By step（3）Middle W/O Reversed micelles chloroform removed under pressure, adds 10 milliliters of steps（2）What is obtained contains Tea Polyphenols
Phosphate buffer solution carry out the 2nd time emulsification, formed w/o type emulsion；Decompression boils off organic solvent, produces liposome.
The measure of the envelop rate of embodiment 2
Free Tea Polyphenols supernatant after the liposome of embodiment 1 is separated, plus distilled water are settled to 100ml, using spectrophotometric
Method determines the amount of free Tea Polyphenols, and packet rate is calculated as follows：
Envelop rate=（Put into TP amounts-free TP amounts）/ input TP amounts * 100%；
Above-mentioned preparation method is repeated, it is respectively 74.54%, 77.72% and 78.79% to measure envelop rate, average envelop rate is
77.02%。
Claims (1)
1. a kind of preparation method of tea polyphenol liposomes, it is characterised in that comprise the following steps：
（1）Appropriate 100 milligrams of soybean lecithin, 20-30 milligrams of cholesterol are weighed, 30ml chloroforms is added and is allowed to dissolve, mixed
Close solution；
（2）Configure the phosphate buffer solution containing Tea Polyphenols：The phosphate buffer solution that configuration pH value is 7.2-7.5 first, it is dense
Spend for 10-20 mMs/milliliter；The ratio in 10 milliliters of phosphate buffer solutions is added to according to 50-100 milligrams of Tea Polyphenols,
The phosphate buffer solution containing Tea Polyphenols is made；
（3）Take step（2）Obtained 10 milliliters of the phosphate buffer solution containing Tea Polyphenols is added to step（1）Obtained mixing is molten
In liquid, the 1st emulsification is carried out, W/O Reversed micelles are formed；
（4）By step（3）Middle W/O Reversed micelles chloroform removed under pressure, adds 10 milliliters of steps（2）What is obtained contains Tea Polyphenols
Phosphate buffer solution carry out the 2nd time emulsification, formed w/o type emulsion；Decompression boils off organic solvent, produces liposome.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710398032.5A CN107049952A (en) | 2017-05-31 | 2017-05-31 | A kind of preparation method of tea polyphenol liposomes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710398032.5A CN107049952A (en) | 2017-05-31 | 2017-05-31 | A kind of preparation method of tea polyphenol liposomes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107049952A true CN107049952A (en) | 2017-08-18 |
Family
ID=59617333
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710398032.5A Pending CN107049952A (en) | 2017-05-31 | 2017-05-31 | A kind of preparation method of tea polyphenol liposomes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107049952A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1823730A (en) * | 2005-12-29 | 2006-08-30 | 谢君 | Molecular coating method of plant polyphenol kind substance |
| CN102389150A (en) * | 2011-10-09 | 2012-03-28 | 中国计量学院 | Method for preparing liposome enveloped by catechin |
| WO2014025672A1 (en) * | 2012-08-04 | 2014-02-13 | Eric Hauser Kuhrts | Water-soluble lipophilic natural compound formulations |
-
2017
- 2017-05-31 CN CN201710398032.5A patent/CN107049952A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1823730A (en) * | 2005-12-29 | 2006-08-30 | 谢君 | Molecular coating method of plant polyphenol kind substance |
| CN102389150A (en) * | 2011-10-09 | 2012-03-28 | 中国计量学院 | Method for preparing liposome enveloped by catechin |
| WO2014025672A1 (en) * | 2012-08-04 | 2014-02-13 | Eric Hauser Kuhrts | Water-soluble lipophilic natural compound formulations |
Non-Patent Citations (2)
| Title |
|---|
| 张志勤等: "茶多酚脂质体研究进展", 《辽宁中医药大学学报》 * |
| 陈荣义: "茶多酚的提取纯化及其改性的研究", 《中国博士学位论文全文数据库 工程科技I辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Choi et al. | Ethosomes and transfersomes for topical delivery of ginsenoside Rh1 from red ginseng: characterization and in vitro evaluation | |
| Zeng et al. | Aloe derived nanovesicle as a functional carrier for indocyanine green encapsulation and phototherapy | |
| CA2879931A1 (en) | Cochleates made with soy phosphatidylserine | |
| KR102177196B1 (en) | Nanoparticle multi-layered lamellar niosomes which is formed by nanoparticlation after being formed large niosomes in a supercritical state and cosmetic composition thereof | |
| Khatibi et al. | Effect of preparation methods on the properties of Zataria multiflora Boiss. essential oil loaded nanoliposomes: characterization of size, encapsulation efficiency and stability | |
| CN110999988A (en) | Preparation method of white tea and tea seed oil microcapsules | |
| CN101822669B (en) | Liposome injection of amoxicillin sodium sulbactam sodium medicinal composition | |
| KR20140069469A (en) | Cosmetic composition for improving atopic dermatitis comprising nano capsule containing natural complex extract and manufacturing method thereof | |
| Nagpal et al. | Designing of a phytosome dosage form with Tecomella undulata as a novel drug delivery for better utilization | |
| CN110507551B (en) | Preparation method of permeation-promoting anti-wrinkle essence preparation containing acetyl hexapeptide-8 | |
| CN108741080A (en) | A kind of microalgae DHA- anthocyanidin biphase liposome and preparation method thereof | |
| CN101816632B (en) | Composite functional liposome and preparation method and application thereof | |
| CN100360115C (en) | Molecular coating method of plant polyphenol kind substance | |
| Jahanfar et al. | Liposomal green tea extract: optimization and physicochemical characterization | |
| CN103976961B (en) | Preparation method and application of reduced glutathione solid lipid nanoparticles | |
| CN102485212A (en) | Sunitinib malate liposome and preparation method thereof | |
| CN107049952A (en) | A kind of preparation method of tea polyphenol liposomes | |
| CN102397242A (en) | Hydrogel containing coenzyme Q10, and cataplasm prepared by hydrogel | |
| CN110251466A (en) | A kind of cannabidiol liposome and preparation process | |
| CN106821789A (en) | A kind of preparation method of B B-complex nourishing cream and its liposomal thing | |
| CN108524364B (en) | Cosmetic active matter nano-carrier package and preparation method thereof | |
| Salunkhe et al. | Herbal liposomes: natural network for targeted drug delivery system | |
| CN102198100B (en) | Carmustine injectable powder and preparation technology | |
| CN104083326B (en) | A kind of preparation method of the liposome wrapping load protein medicaments | |
| CN103861090A (en) | Protein or peptide containing hydrophobic solution, preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170818 |