CN107049294B - Microvascular ultrastructural imaging device - Google Patents
Microvascular ultrastructural imaging device Download PDFInfo
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- CN107049294B CN107049294B CN201710053312.2A CN201710053312A CN107049294B CN 107049294 B CN107049294 B CN 107049294B CN 201710053312 A CN201710053312 A CN 201710053312A CN 107049294 B CN107049294 B CN 107049294B
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- 238000003384 imaging method Methods 0.000 title claims abstract description 78
- 238000012545 processing Methods 0.000 claims abstract description 4
- 230000000903 blocking effect Effects 0.000 claims description 12
- 239000008280 blood Substances 0.000 claims description 10
- 210000004088 microvessel Anatomy 0.000 claims description 9
- 230000005540 biological transmission Effects 0.000 abstract description 6
- 230000017531 blood circulation Effects 0.000 description 18
- 238000005457 optimization Methods 0.000 description 9
- 210000000601 blood cell Anatomy 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 6
- 230000004089 microcirculation Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 4
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- 238000000149 argon plasma sintering Methods 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 210000003556 vascular endothelial cell Anatomy 0.000 description 2
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- 208000027418 Wounds and injury Diseases 0.000 description 1
- 210000002565 arteriole Anatomy 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000008822 capillary blood flow Effects 0.000 description 1
- 210000001043 capillary endothelial cell Anatomy 0.000 description 1
- 208000010877 cognitive disease Diseases 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
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- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 210000004925 microvascular endothelial cell Anatomy 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
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- 238000011160 research Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 210000000264 venule Anatomy 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/02—Detecting, measuring or recording pulse, heart rate, blood pressure or blood flow; Combined pulse/heart-rate/blood pressure determination; Evaluating a cardiovascular condition not otherwise provided for, e.g. using combinations of techniques provided for in this group with electrocardiography or electroauscultation; Heart catheters for measuring blood pressure
- A61B5/026—Measuring blood flow
- A61B5/0261—Measuring blood flow using optical means, e.g. infrared light
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0062—Arrangements for scanning
- A61B5/0064—Body surface scanning
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Abstract
The invention provides a microvascular ultrastructural imaging device, which is used for forming two-dimensional images of microvascular ultrastructural information in a body to be detected, and comprises a light source, an annular light unit for converting linearly polarized light emitted from the light source into annular circularly polarized light, a lens unit and an imaging unit for processing reflected light with microvascular information after being reflected by the lens unit to form two-dimensional images; the lens unit comprises an imaging lens arranged at the inner center of the lens unit and a conical mirror surface arranged around the imaging lens in a surrounding way, and the tip of the mirror surface is the light emergent end. The device can convert the light transmitted to the object to be detected into circularly polarized light, and improves the light transmission capability and imaging definition.
Description
Technical Field
The invention relates to the field of micro-vessel ultrastructural observation, in particular to a micro-vessel ultrastructural imaging device.
Background
Microcirculation refers to the process by which blood and tissue cells exchange substances between the arterioles and venules. Microcirculation is the integrity of life morphology and metabolism, and is an indispensable condition for maintaining normal functions of human organs. Through the research of microcirculation, the special functions of various organs of the human body can be further known, the pathogenesis of the cognitive diseases is facilitated, and the disease prevention, diagnosis and treatment are facilitated. For example, diseases including diabetes, hypertension, coronary heart disease, etc. all cause conditions of microcirculation including parameters such as capillary diameter, capillary density, and micro blood flow velocity in the capillaries, and ultrastructural changes such as capillary endothelial cells and blood cells. Therefore, the microcirculation quality is grasped by knowing the ultramicro structure of the micro blood vessels, and the method has extremely important effects on diagnosis and treatment of various diseases.
In the prior art, the micro-blood flow imaging technology includes using lateral flow dark field imaging, that is, annular LED illumination is firstly adopted to surround the periphery of an imaging microscope lens, the light source emitted by the LED is light with a specific wavelength, but not polarized light, and the LED light irradiates the skin annularly from the periphery of the microscope lens, and scatters inside the skin while scattering occurs on the surface of the skin. Because the micro lens is very close to the skin, the light which is annularly irradiated on the skin and reflected back is difficult to enter the lens for imaging, the irradiation direction of the internal scattered light is random, and a part of the light irradiates on the micro lens for imaging on the CCD, so that the direct imaging of micro blood flow by skin surface reflection is avoided.
However, according to the known polarization principle, that is, polarized light has better transmission ability in the skin than unpolarized light, and circularly polarized light has better transmission ability and imaging definition than linearly polarized light, in the above-mentioned technique, since the projection is performed using unpolarized light, the transmission ability of light into the skin and the imaging definition are insufficient, and it is difficult to obtain a clear image in a simple manner, the above-mentioned technique cannot clearly image the microvascular ultrastructure and is difficult to observe.
Disclosure of Invention
In order to solve the above problems, the present invention provides a microvascular ultrastructural imaging device capable of converting light transmitted into a body to be measured into circularly polarized light, improving light transmittance and imaging definition.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the microvascular ultrastructural imaging device is used for forming two-dimensional images of microvascular ultrastructural imaging information in a body to be detected, and comprises a light source capable of emitting linearly polarized light, a ring light unit capable of converting the linearly polarized light emitted from the light source into annular circularly polarized light, and a lens unit capable of injecting the annular circularly polarized light emitted from the ring light unit into one point to form concentrated light; and an imaging unit for processing the reflected light with micro blood flow information after being reflected by the lens unit to form a two-dimensional image; the annular circularly polarized light emitted from the annular light unit is transmitted into the body to be detected through the lens unit, and after being scattered inside the body to be detected, the light carries micro blood flow information in the body to be detected to the imaging unit so as to form a two-dimensional image.
As a further optimization of the invention, the lens unit is provided with an incident end capable of receiving annular circularly polarized light and an emergent end capable of emitting concentrated light, the lens unit comprises an imaging lens arranged at the inner center of the lens unit and conical mirror surfaces arranged around the imaging lens in a surrounding way, and the tip of the mirror surfaces is the emergent end of the light; the annular circularly polarized light emitted from the annular light unit irradiates on the conical mirror surface of the lens unit, is intensively injected to the emergent end to be transmitted into the body to be detected, and after being scattered inside the body to be detected, light carries microvascular ultrastructural information in the body to be detected and is injected into the imaging unit from the imaging lens to form a two-dimensional image.
As a further optimization of the present invention, the ring light unit includes a collimator lens capable of forming parallel light from divergent light emitted in the light source, an aperture capable of converting linearly polarized light into circularly polarized light, and a beam splitting unit capable of reflecting the circularly polarized light in the aperture to the inside of the lens unit, the collimator lens, the aperture, and the beam splitting unit being sequentially disposed between the light source and the lens unit at intervals, wherein the aperture includes a light blocking body having a circular shape disposed at a central portion, and a light adjusting unit disposed around the light blocking body, and a diameter of the light blocking body is larger than a diameter of the imaging lens in a central portion of the lens unit and smaller than a diameter of an incident end of the lens unit.
As a further optimization of the present invention, the light-splitting unit is one of a non-polarizing light-splitter or a semi-transparent mirror.
As a further optimization of the present invention, a light shielding plate that blocks linear light transmitted through the light splitting unit is provided at a side portion of the light splitting unit.
As a further optimization of the invention, the aperture further comprises an opaque ring capable of avoiding light leakage, and the opaque ring is arranged outside the dimming unit.
As a further optimization of the present invention, the dimming unit is one of a 1/4 phase difference film or a 1/4 wavelength plate.
As a further optimization of the invention, the left and right sides of the aperture are provided with anti-reflection layers capable of preventing visible light from reflecting.
As a further optimization of the invention, the imaging unit comprises an aspheric lens arranged above the incident end of the lens unit, so as to output the nonparallel lens unit carrying micro blood flow information of the object to be tested to modulate light to form a light beam; the digital camera is arranged above the aspheric lens and senses the light beam formed by the aspheric lens so as to form a two-dimensional image according to the information of the object to be detected carried on the light beam.
As a further optimization of the invention, the lens bottom of the lens unit is sleeved with a lens sleeve which can be attached to the surface of the object to be measured.
Compared with the prior art, the invention has the advantages and positive effects that:
1. the microvascular ultrastructural imaging device adopts the light source capable of emitting linearly polarized light, so that the emitted light is the linearly polarized light, and further, the circularly polarized light passes through the lens unit which is not easy to absorb reflected light and is transmitted into the body to be detected by using the annular light unit capable of converting the linearly polarized light into circularly polarized light, and the transmission capacity of the light and the imaging definition are improved by the matched use of the light source, the annular light unit and the lens unit;
2. the invention relates to a microvascular ultrastructural imaging device, which adopts a lens unit with an imaging lens arranged in the middle and a conical mirror surface arranged around the imaging lens, wherein light rays emitted from the annular light unit are emitted around the conical mirror surface of the lens unit and concentrated to the tip end position of the lens unit, and the light rays are concentrated to irradiate in an inclined plane, so that when a body to be measured is a plane, plane reflected light can be symmetrically reflected to the light source emitting end and can not be received by the lens unit, but for a light scattering body, the propagation direction of scattered light is random, and therefore, the light rays are easily received by the lens unit to finally image.
3. The microvascular ultrastructural imaging device effectively reduces imaging background noise caused by reflected light on the surface of the surface to be measured, and simultaneously reduces the volume of equipment.
Drawings
FIG. 1 is a schematic diagram of a first embodiment of a micro-blood flow imaging device according to the present invention;
FIG. 2 is a schematic view of an optical path of a first embodiment of a micro-blood flow imaging device according to the present invention;
FIG. 3 is a schematic diagram of a second embodiment of a micro-blood flow imaging device according to the present invention;
FIG. 4 is a schematic view of a second embodiment of a micro-blood flow imaging device according to the present invention;
FIG. 5 is a schematic diagram of a second embodiment of a spectroscopic unit according to the present invention;
FIG. 6 is a schematic view of an internal optical path of a lens unit according to the present invention;
fig. 7 is a schematic view of an aperture.
In the above figures: 1. a light source; 2. a ring light unit; 21. a collimating lens; 22. an aperture; 221. a light blocking body; 222. a 1/4 retardation film; 23. a half-lens; 231. a non-polarizing beam splitter; 24. a reflective mirror; 3. a lens unit; 31. an imaging lens; 32. a mirror surface; 4. an aspherical lens; 5. a digital camera; 6. a support frame; 7. a body to be measured; 8. a light shielding plate.
Detailed Description
The present invention will be specifically described below by way of exemplary embodiments. It is to be understood that elements, structures, and features of one embodiment may be beneficially incorporated in other embodiments without further recitation.
In the description of the present invention, it should be noted that the height direction of the micro blood flow imaging device is the vertical direction after the micro blood flow imaging device is installed; the terms "inner", "outer", "upper", "lower", "front", "rear", etc. indicate orientations or positional relationships based on the positional relationships shown in fig. 1, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the present invention, the micro blood flow information in the subject includes the following information: the distribution density of the microvessels, the diameter of the microvessels, the flow rate of the microvessels or capillary blood flow, the blood cells in the blood flow, the endothelial cells of the microvessels and other cell microstructures.
Referring to fig. 1 and 3, there are schematic structural views of a first embodiment and a second embodiment of a micro-blood flow imaging apparatus according to the present invention. As shown in fig. 1 and fig. 3, the light source 1 for forming a two-dimensional image of micro blood flow information in the body 7 to be measured includes a light source 1 capable of emitting linearly polarized light, where the light source 1 may be a laser or an LED light source with a surface coated with a linearly polarized film, and the light source is limited only by that the light emitted by the light source is linearly polarized light, and a specific single form is adopted without specific limitation; in addition, the LED light source using the plated wire polarizing film has the advantages that the service life of the LED is long, the irradiation is uniform, and speckle noise is avoided; further comprising a ring light unit 2 for converting the linear polarized light emitted from the light source 1 into circular polarized light, and a lens unit 3 for forming a condensed light by injecting the circular polarized light emitted from the ring light unit 2 into a point; and an imaging unit for processing the reflected light with micro blood flow information after being reflected by the lens unit 3 to form a two-dimensional image.
Referring also to fig. 5, fig. 5 is a schematic view of the optical path inside the lens unit in the present invention. Referring to fig. 5, the lens unit 3 may be one of a microscope objective lens or a zoom lens, where the microscope objective lens includes a limited-distance-correction microscope objective lens and an infinity-correction microscope objective lens, which has a very high optical resolution, and can implement clear imaging of a macroscopic structure of a portion of an observed object and an internal microstructure, such as blood cells and microvascular endothelial cells; the zoom lens can adjust the magnification to expand and reduce the observation range, so that not only can the observed object be observed in a macroscopic structure, but also the observed object can be subjected to clear imaging of internal microstructures such as blood cells and vascular endothelial cells; when the lens unit is selected, the microscope objective lens and the variable focus lens can be selectively used according to the purpose and the range of use of the device. For example, when the distribution density of the micro-blood vessels in a large area is required to be counted and the blood flow condition of blood cells is required to be observed to be analyzed, a variable-focus lens is used; only the blood cells and the vascular endothelial cells in the blood vessel are required to be observed, and when the imaging quality requirement is high, a microscope objective lens is used.
With continued reference to fig. 5, the lens unit 3 has an incident end capable of receiving circularly polarized light and an exit end capable of emitting concentrated light, the incident end is an upper end of the lens unit 3, the exit end is a lower end of the lens unit 3, the lens unit 3 includes an opaque imaging lens 31 disposed at an inner center of the lens unit, and conical mirror surfaces 32 disposed around the imaging lens 31, and a tip of the mirror surface 32 is an exit end of the light; because the mirror surface is conical, the annular light rays incident from the incident end of the lens unit are converged at one point through the mirror surface, annular circularly polarized light emitted from the annular light unit 2 irradiates on the conical mirror surface of the lens unit and is intensively emitted to the emergent end to be transmitted on the body 7 to be detected, and after the light rays are scattered inside the body 7 to be detected, the light rays carry microvascular ultrastructural imaging information and are emitted into the imaging unit from the imaging lens to form a two-dimensional image.
The conical mirror surface may be a transparent prism, and if the conical mirror surface is a prism, the annular light incident from the incident end of the lens unit is refracted through the mirror surface and then converged from one point to the emergent end; the conical mirror surface can also be a reflecting mirror, and if the conical mirror surface is a reflecting mirror, annular light rays incident from the incident end of the lens unit are reflected by the mirror surface and then converged at one point to the emergent end. Therefore, the conical mirror surface is not limited to the material, and the specific light path where the light beam occurs is not limited, and the light beam can be emitted from the incident end and concentrated and converged at the emitting end only by being satisfied.
Referring further to fig. 1 to 6, the ring light unit 2 includes a collimator lens 21 that can form parallel light from divergent light emitted in the light source 1, a diaphragm 22 that can convert linearly polarized light into circularly polarized light, and a spectroscopic unit that can reflect circularly light in the diaphragm 22 to the inside of the lens unit, the collimator lens 21, the diaphragm 22, and the spectroscopic unit being sequentially disposed at intervals between the light source 1 and the lens unit 3, wherein the diaphragm 22 includes a light blocking body 221 disposed in a circular shape in a central portion, and a dimming unit disposed around the light blocking body, which may be a 1/4 phase difference film or a 1/4 wavelength plate, the 1/4 phase difference film 222 being a dimming unit being shown in the figure, the diameter of the light blocking body 221 being larger than the diameter of the imaging lens 31 in the middle of the lens unit 3 and smaller than the diameter of the incident end of the lens unit 3. The light-splitting unit may be the half-lens 23 in fig. 4 or the non-polarizing beam splitter 231 in fig. 5, where the non-polarizing beam splitter or the half-lens may partially transmit polarized light (including circularly polarized light and linearly polarized light) or partially reflect the non-polarized light. The unpolarized beam splitter is functionally identical to the half-lens, but is only shaped differently, i.e. the unpolarized beam splitter is a cube and the half-lens is planar.
Meanwhile, in the invention, the side part of the light splitting unit is also provided with the light shielding plate 8, and the light shielding plate 8 can absorb the linear light transmitted through the light splitting unit.
The light adjusting unit arranged in the aperture 22 changes linearly polarized light passing through the light adjusting unit into circularly polarized light due to the delay of the light adjusting unit on the phase of the light, meanwhile, an imaging lens is arranged in the lens unit, the side part is a conical mirror surface, a light blocking body which is not light transmitting is correspondingly arranged in the middle of the aperture, the light blocking body prevents the light from propagating forwards, and the light adjusting unit part enables the light to pass through, so that the light reflected by the aperture is annular.
Referring to fig. 2, 4 and 5, as shown in the drawings, the micro-blood flow imaging device of the present invention has the following optical paths: the light source 1 emits linearly polarized light, which is divergent light, which passes through the collimator lens 21 to form parallel light; the parallel linearly polarized light forms annular circularly polarized light after passing through the aperture 22, the circularly polarized light enters an incident end of the lens unit 3 through reflection of the half lens 23, the annular circularly polarized light is converged at an emergent end of the lens unit 3 through a conical mirror surface 32 of the lens unit 3, and then is transmitted into the body 7 to be detected; since the light is intensively irradiated onto the object 7 in the inclined plane, when the object 7 is a plane, the plane reflected light is symmetrically reflected to the exit end of the lens unit and is not received by the lens unit, but for the light scattering object, the propagation direction of the scattered light is random, so that the scattered light is easily received by the lens unit to be finally imaged. The invention fully utilizes the better transmission capability and imaging definition of the circularly polarized light than the linearly polarized light, so that the micro blood flow imaging device can image more clearly when observing micro blood flow.
In addition, in order to further avoid light leakage of the device and prevent injury to the user, the aperture 22 further includes an opaque ring capable of avoiding the emission of excessive light, the opaque ring is disposed outside the light modulation unit, and the diameter of the inner ring of the opaque ring is larger than the diameter of the incident end of the lens unit.
In order to prevent unnecessary specular reflection, the left and right sides of the aperture are provided with anti-reflection layers capable of preventing reflection of visible light, and the anti-reflection layers may be AR plating films.
Referring to fig. 1 and 3 in detail, the imaging unit includes an aspheric lens 4 disposed above the incident end of the lens unit, and the lens focus of the aspheric lens 4 coincides with the backward focus of the lens unit 3, so as to modulate the output light of the non-parallel lens unit carrying the micro blood flow information of the object to be measured into a light beam; a digital camera 5 is arranged above the aspherical lens 4, the digital camera 5 is provided with a CMOS or CCD sensor, and the digital camera 5 senses a light beam formed by the aspherical lens 4 to form a clear two-dimensional image according to information of a body to be measured carried on the light beam. In the above, the position of the aspherical lens 4 depends only on the lens unit, and thus the position of the aspherical lens 4 may be disposed above the half lens as shown in fig. 1 or below the half lens as shown in fig. 3.
The main difference between the first embodiment and the second embodiment of the present invention is that a mirror 24 is arranged obliquely between the half mirror 23 and the aperture 22, where the mirror 24 is preferably arranged at an angle of 45 degrees, and the function of the mirror is to emit the light rays emitted from the aperture 22 onto the half lens 23. In the first embodiment of the present invention, when only one half lens is shown, the half lens is obliquely disposed at the outgoing line end of the aperture, and the reflecting surface of the half lens faces the incident end of the lens unit, so as to reflect the light transmitted through the aperture into the lens unit; in a second embodiment of the invention, a mode of auxiliary arrangement of a reflector is shown, wherein the reflector is arranged opposite to the outgoing line end of the aperture, the reflecting surface of the reflector is opposite to the half lens, and the reflecting surface of the half lens is opposite to the incident end of the lens unit. The above two embodiments are merely examples, and in fact, by way of the above examples, we can further derive that the number of reflectors used for assisting light emission is not particularly limited, as long as the number of reflectors used for assisting light emission is satisfied that the half lens can directly or indirectly receive light emitted from the aperture, and the reflecting surface of the half lens faces the incident end of the lens unit, and that several reflectors are arranged in the middle for reflecting, which depends on the frame structure of a specific micro blood flow imaging device and the internal arrangement manner, and in the second embodiment of the present invention, it is obvious that the arrangement of the structure is more advantageous by adding one reflector.
The second embodiment has the advantage that the reflecting mirror is used for reflecting the laser light path at 90 degrees, so that the digital camera and the light source can keep the same direction, a communication line is conveniently led out from one interface, in addition, the reflecting mirror and the semi-transparent mirror are both fixed in the supporting frame, the supporting frame is connected by four turnbuckles, and a circle of rubber is surrounded at the connecting gap for preventing water drops.
In order to facilitate the operation of the lens unit, a lens cover capable of being attached to the surface of the object to be measured is provided at the bottom of the lens unit 3.
The invention further comprises a support frame 6 arranged outside the light source, the annular light unit and the lens unit, wherein the support frame 6 is arranged outside the optical elements in an annular mode, and the support frame comprises but is not limited to the light source, the annular light unit and the lens unit, and can also comprise an aspheric lens, a digital camera and the like. The lens unit and the like may be mounted on the support frame 6 with screws, and the aspherical lens and the diaphragm may be fixed inside the micro blood flow imaging apparatus with two ring screws. Meanwhile, the lens unit is connected with the semi-transparent lens through screws, and the telescopic lens barrel is twisted by hands, so that the distance between the digital camera and the aspheric lens can be changed, and the distance between the aspheric lens and the lens unit is unchanged, thus ensuring simple adjustment of focal length during use.
The micro blood flow imaging device can realize annular circularly polarized light by only using one light source capable of emitting linearly polarized light, and effectively reduces imaging background noise caused by surface reflection light by utilizing the characteristic of a conical mirror surface, and simultaneously reduces the volume of equipment.
Claims (6)
1. The utility model provides a micro-vessel ultrastructural imaging device for form two-dimensional image with micro-vessel ultrastructural information in the body that awaits measuring, its characterized in that: comprising
A light source capable of emitting linearly polarized light,
a ring light unit converting linearly polarized light emitted from the light source into circularly polarized light,
the lens unit can shoot annular circularly polarized light emitted from the annular light unit into one point to form concentrated light; and an imaging unit for processing the reflected light with the pico-blood vessel ultrastructural information after being reflected by the lens unit to form a two-dimensional image;
the annular circularly polarized light emitted from the annular light unit is transmitted into the body to be detected through the lens unit, and after being scattered inside the body to be detected, the light carries microvascular ultrastructural information in the body to be detected to the imaging unit so as to form a two-dimensional image;
the lens unit is provided with an incident end capable of receiving annular circularly polarized light and an emergent end capable of emitting concentrated light, and comprises an imaging lens arranged at the inner center of the lens unit and conical mirror surfaces arranged around the imaging lens in a surrounding manner, and the tip of each mirror surface is the emergent end of the light; the annular circularly polarized light emitted from the annular light unit irradiates on a conical mirror surface of the lens unit, and is intensively emitted to the emergent end to be transmitted into the body to be detected, and after being scattered inside the body to be detected, light carries microvascular ultrastructural information in the body to be detected and is emitted to the imaging unit from the imaging lens to form a two-dimensional image;
the annular light unit comprises a collimating lens capable of forming parallel light by divergent light emitted from the light source, an aperture capable of converting linearly polarized light into annular circularly polarized light and a light splitting unit capable of reflecting the annular light in the aperture to the inside of the lens unit, wherein the collimating lens, the aperture and the light splitting unit are sequentially arranged between the light source and the lens unit at intervals, the aperture comprises a light blocking body which is arranged at the central part and is circular, and a light adjusting unit which is arranged in an annular manner, and the diameter of the light blocking body is larger than that of an imaging lens in the middle of the lens unit and smaller than that of an incident end of the lens unit;
the imaging unit comprises an aspheric lens arranged above the incident end of the lens unit, so that the non-parallel lens unit carrying the micro-vascular ultrastructural imaging information is output to be modulated to form a light beam; a digital camera is arranged above the aspheric lens, and senses a light beam formed by the aspheric lens to form a two-dimensional image according to micro-vessel ultrastructural imaging information carried on the light beam;
the lens bottom of the lens unit is sleeved with a lens sleeve which can be attached to the surface of the object to be measured.
2. The microvascular ultrastructural imaging device of claim 1, wherein: the light-splitting unit is one of a non-polarized light-splitting device or a semi-transparent mirror.
3. The microvascular ultrastructural imaging device of claim 2, wherein: the side part of the light splitting unit is provided with a light shielding plate for blocking linear light transmitted through the light splitting unit.
4. The microvascular ultrastructural imaging device of claim 1, wherein: the aperture also comprises an opaque ring capable of avoiding light leakage, and the opaque ring is arranged outside the dimming unit.
5. The microvascular ultrastructural imaging device of claim 4, wherein: the left and right sides of the aperture are provided with anti-reflection layers capable of preventing visible light from reflecting.
6. The microvascular ultrastructural imaging device of claim 1, wherein: the light adjusting unit is one of a 1/4 phase difference film or a 1/4 wavelength plate.
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| CN103210336A (en) * | 2010-10-20 | 2013-07-17 | 株式会社尼康 | Microscope system |
| CN103445764A (en) * | 2013-09-04 | 2013-12-18 | 广州医软智能科技有限公司 | Device and method for monitoring microcirculation imaging |
| CN103926225A (en) * | 2014-03-28 | 2014-07-16 | 浙江大学 | Fluorescence emitting differential microscopy method and device based on evanescent wave lighting |
| CN104783767A (en) * | 2015-04-10 | 2015-07-22 | 重庆理工大学 | Device and method for detecting human body microcirculation by means of orthogonal polarization spectral imaging |
| CN207118900U (en) * | 2017-01-24 | 2018-03-20 | 青岛大学附属医院 | Ultrastructure imaging device |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103210336A (en) * | 2010-10-20 | 2013-07-17 | 株式会社尼康 | Microscope system |
| CN103445764A (en) * | 2013-09-04 | 2013-12-18 | 广州医软智能科技有限公司 | Device and method for monitoring microcirculation imaging |
| CN103926225A (en) * | 2014-03-28 | 2014-07-16 | 浙江大学 | Fluorescence emitting differential microscopy method and device based on evanescent wave lighting |
| CN104783767A (en) * | 2015-04-10 | 2015-07-22 | 重庆理工大学 | Device and method for detecting human body microcirculation by means of orthogonal polarization spectral imaging |
| CN207118900U (en) * | 2017-01-24 | 2018-03-20 | 青岛大学附属医院 | Ultrastructure imaging device |
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