CN107047482B - A kind of Odontotermes formosanus colouring method - Google Patents
A kind of Odontotermes formosanus colouring method Download PDFInfo
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- CN107047482B CN107047482B CN201710179944.3A CN201710179944A CN107047482B CN 107047482 B CN107047482 B CN 107047482B CN 201710179944 A CN201710179944 A CN 201710179944A CN 107047482 B CN107047482 B CN 107047482B
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- 241000866536 Odontotermes formosanus Species 0.000 title claims abstract description 66
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000004040 coloring Methods 0.000 title claims abstract description 15
- 238000004043 dyeing Methods 0.000 claims abstract description 40
- 239000003086 colorant Substances 0.000 claims abstract description 23
- 239000000843 powder Substances 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 11
- 239000002023 wood Substances 0.000 claims abstract description 10
- 241000609240 Ambelania acida Species 0.000 claims abstract description 8
- 229920002261 Corn starch Polymers 0.000 claims abstract description 8
- 244000062793 Sorghum vulgare Species 0.000 claims abstract description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 8
- 229930006000 Sucrose Natural products 0.000 claims abstract description 8
- 239000010905 bagasse Substances 0.000 claims abstract description 8
- 239000008120 corn starch Substances 0.000 claims abstract description 8
- 229940099112 cornstarch Drugs 0.000 claims abstract description 8
- 235000013312 flour Nutrition 0.000 claims abstract description 8
- 235000019713 millet Nutrition 0.000 claims abstract description 8
- 239000005720 sucrose Substances 0.000 claims abstract description 8
- 239000000975 dye Substances 0.000 claims description 28
- 239000012153 distilled water Substances 0.000 claims description 18
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims description 18
- 239000006004 Quartz sand Substances 0.000 claims description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 13
- 238000012545 processing Methods 0.000 claims description 13
- 239000001044 red dye Substances 0.000 claims description 12
- 244000082946 Tarchonanthus camphoratus Species 0.000 claims description 11
- 235000005701 Tarchonanthus camphoratus Nutrition 0.000 claims description 11
- DSSYKIVIOFKYAU-XCBNKYQSSA-N (R)-camphor Chemical compound C1C[C@@]2(C)C(=O)C[C@@H]1C2(C)C DSSYKIVIOFKYAU-XCBNKYQSSA-N 0.000 claims description 10
- 229940043267 rhodamine b Drugs 0.000 claims description 9
- 238000005507 spraying Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 7
- 235000011613 Pinus brutia Nutrition 0.000 claims description 7
- 241000018646 Pinus brutia Species 0.000 claims description 7
- 239000003550 marker Substances 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical compound Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 claims description 5
- 235000011609 Pinus massoniana Nutrition 0.000 claims description 4
- 241000018650 Pinus massoniana Species 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 15
- 241000238631 Hexapoda Species 0.000 abstract description 3
- -1 pollen pini Substances 0.000 abstract description 3
- 238000011534 incubation Methods 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 abstract description 2
- 239000000049 pigment Substances 0.000 description 6
- KVYRCBOUKXJXDK-UHFFFAOYSA-N 3,4-dimethylphenazine-1,2-diamine hydrochloride Chemical compound Cl.C1=CC=CC2=NC3=C(C)C(C)=C(N)C(N)=C3N=C21 KVYRCBOUKXJXDK-UHFFFAOYSA-N 0.000 description 5
- 230000003542 behavioural effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 244000144730 Amygdalus persica Species 0.000 description 1
- 241000246868 Astilbe japonica Species 0.000 description 1
- 244000111489 Gardenia augusta Species 0.000 description 1
- 244000284380 Hibiscus rosa sinensis Species 0.000 description 1
- 241000256602 Isoptera Species 0.000 description 1
- 240000005819 Magnolia denudata Species 0.000 description 1
- 235000016094 Magnolia denudata Nutrition 0.000 description 1
- 241001481296 Malus spectabilis Species 0.000 description 1
- 241000866537 Odontotermes Species 0.000 description 1
- 244000242564 Osmanthus fragrans Species 0.000 description 1
- 235000019083 Osmanthus fragrans Nutrition 0.000 description 1
- 244000141698 Prunus lannesiana Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 244000277586 Prunus pissardii Species 0.000 description 1
- 235000009836 Prunus pissardii Nutrition 0.000 description 1
- 235000014001 Prunus serrulata Nutrition 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 235000000664 Rosa chinensis Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- DEDOPGXGGQYYMW-UHFFFAOYSA-N molinate Chemical compound CCSC(=O)N1CCCCCC1 DEDOPGXGGQYYMW-UHFFFAOYSA-N 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to harmful insect Prevention Technique fields.The invention discloses a kind of Odontotermes formosanus colouring methods, its by coloring agent prepare, phagostimulant prepare and Odontotermes formosanus culture and etc. form, wherein, coloring agent is made of water soluble dyestuffs and tagged fluorescent agent, phagostimulant is made of millet flour, cornstarch, pollen pini, wood powder, dry bark, bagasse and sucrose, and Odontotermes formosanus, which is dyed, keeps dark and temperature and humidity appropriate in incubation.The present invention has preferable dyeing effect, and Odontotermes formosanus repellency is smaller；Each phase identification degree is preferable after dyeing, can fast and accurately differentiate labeled individual；To being safe from harm property of Odontotermes formosanus, the mobility for being colored Odontotermes formosanus will not be reduced.
Description
Technical field
The present invention relates to harmful insect Prevention Technique fields, more particularly, to a kind of Odontotermes formosanus colouring method.
Background technique
Odontotermes formosanus is a kind of insect of Isoptera Termitidae odontotermes, is distributed in Chinese the Yellow River, each on the south the Changjiang river
Province ,city and area, main harm oriental cherry, plum blossom, can also endanger sweet osmanthus, peach blossom, wide yulan, bedleaf cherry plum, Chinese rose, gardenia, Malus spectabilis,
The flowers and trees such as rose, wax plum, Reeves spiraea are a kind of dwelling property of soil pests, mainly endanger bark and shallow layer of wood and root with worker ant,
It causes to form bulk ant road outside killed trunk, growing way decline, after invading xylem, then trunk is withered；Especially to seedling, easily
It causes death.Habit, production and the scope of activities for studying Odontotermes formosanus are an important process for preventing Odontotermes formosanus；It passes
The labeling method of system Odontotermes formosanus is that simple conventional dyes spraying or pigment are fed, in conventional dyes spray coating method, dyestuff
In often containing film forming agent, auxiliary agent and some solvents etc., adverse effect can be generated to Odontotermes formosanus in use, it is white to influence black wing soil
The growth of ant, reduces the activity of Odontotermes formosanus, or even causes it interim dead, will affect Odontotermes formosanus habit tune in this way
The accuracy looked into；During pigment is fed, Odontotermes formosanus, Odontotermes formosanus pair are usually fed using the filter paper after pigment impregnates
Pigment has repellency, is often unwilling to gnaw the filter paper after pigment impregnates, and dyeing time is long, and dyeing effect is poor, dyeing effect
Fruit is unobvious, and in field investigation, and the Odontotermes formosanus after common pigments are fed distinguishes that difficulty is big, it is difficult to be accurately finished
Correlation study.
Summary of the invention
To solve the above problems, having good dyeing effect, repellency smaller the present invention provides one kind and will not
Reduce the active colouring method of Odontotermes formosanus being colored.
To achieve the above object, the technical solution adopted by the present invention is as follows；
A kind of Odontotermes formosanus colouring method, comprising the following steps:
A) coloring agent is prepared: being taken distilled water, water-soluble dye and tagged fluorescent agent, is configured to 0.1~0.5wt%'s respectively
The dye solution of fluorescent marker solution and 0.8~2.4wt%；Then by dye solution and fluorescent marker solution by 1:0.5~2
Weight ratio mixing, is made coloring agent；
B) phagostimulant is prepared: weighing 10 parts of millet flours, 3~5 parts of cornstarch, 1~2 part of pollen pini, 5~7 by weight
Part wood powder, 4~9 parts of dry barks, 2~5 portions of bagasse and 5~8 portions of sucrose, it is dry at 30~45 DEG C after above-mentioned raw materials are mixed
Processing 30~40 minutes, is then crushed to 80~120 mesh, and phagostimulant is made；
C) it dyes bait preparing: phagostimulant is mixed with coloring agent by the weight ratio of 1:1.1~1.5,3~filter after five minutes
Liquid and drying and processing 30~60 minutes at 60~70 DEG C are gone to, dyeing bait is made；
D) dyeing bait is laid in culture dish, then launches the Odontotermes formosanus of crawl thereto, and spray thereto
Appropriate distilled water makes the relative humidity in culture dish be maintained at 80~85%, closes the lid, cultivates in darkroom, keeps temperature
It is 15~30 DEG C；
E) Odontotermes formosanus is cultivated 3~7 days, it is daily as wherein spraying appropriate distilled water, make the relative humidity in culture dish
It is maintained at 80~85%；
F) after the completion of dyeing culture, the Odontotermes formosanus of dyed label is removed.
In colouring method of the present invention, using the side of coloring agent and phagostimulant cooperation production dyeing bait feeding Odontotermes formosanus
Method is dyed.Coloring agent mainly selects the common dyeing agent for not having fluorescent effect and tagged fluorescent agent to form, Odontotermes formosanus
The purpose of dyeing is to be able to quickly find labeled individual in Odontotermes formosanus life habit investigation,
There are two the periods in entire research process to need using dye marker, first is that need to check dyeing effect after dyeing, second is that
Labeled individual is distinguished when investigating habit；In the first stage, due to being to check dyeing effect with the naked eye indoors under environment
It differentiates, and in second stage, it often needs to be distinguished under field either some more complicated environment, therefore utilize
Fluorescent marker is relatively convenient efficiently, but general fluorescent marker needs to be excited with special light sources such as ultraviolet lights, ultraviolet light
It can generate unpredictable influence on labeled individual again, therefore add two kinds of dyeing in the coloring agent that uses of the present invention
Marker, examining dyeing effect in the first stage is that fluorescence mark is used in second stage behavioral study using common dyeing agent
Remember agent, efficiently and accurately completes the behavioral study of entire Odontotermes formosanus.
Since Odontotermes formosanus has repellency to common dyestuff and tagged fluorescent agent, also need to add some lure
Food agent is to promote Odontotermes formosanus intake dyestuff and tagged fluorescent agent, using millet flour and cornstarch as main original in phagostimulant
Material, is then aided with attractive group of some pairs of Odontotermes formosanus and is grouped as phagostimulant；According to the habit of Odontotermes formosanus and grind
Study carefully, has selected pollen pini, wood powder, bark, bagasse and sucrose as food calling component.
In Odontotermes formosanus feeding incubation, the dark, humidity and temperature appropriate of environment are kept.After study,
Temperature is advisable with 15~30 DEG C, and relative humidity is advisable with 80~85%.
Preferably, water soluble dyestuffs are one of neutral red dye or alkaline red dye.
It is that one kind influences minimum organic dyestuff on Odontotermes formosanus that dimethyl diaminophenazine chloride is red with alkalinity, in Odontotermes formosanus body
Both being not easily decomposed will not cause damages to Odontotermes formosanus, will not influence the life habit of Odontotermes formosanus.
Preferably, tagged fluorescent agent is one of rhodamine or rhodamine B.
Rhodamine and rhodamine B are a kind of with fluorescent material under ultraviolet environments, are presented under ultraviolet environments apparent yellow
Green is red, can efficiently distinguish easily.
Preferably, wood powder is that perhaps the camphorwood powder dry bark is masson pine bark or camphorwood skin to pine powder.
Preferably, wood powder is pine powder, the dry bark is camphorwood skin.
Research has shown that the combination of pine powder and camphorwood skin has bigger temptation to Odontotermes formosanus, suitably adds in proportion
Add with good phagostimulating effect.
Preferably, before dyeing bait is laid in culture dish, first tile in culture dish one layer of 14~16 mesh in step d
Quartz sand, the quartz sand is before laying first through sterilization processing.
Culture dish bottom is laid with appropriate amount of quartz sand in advance, can Odontotermes formosanus guarantee have good activity empty under bait
Between, promote it to gnaw bait.
Therefore, the invention has the following advantages:
(1) there is preferable dyeing effect, Odontotermes formosanus repellency is smaller；
(2) each phase identification degree is preferable after dyeing, and can fast and accurately differentiate labeled individual；
(3) to being safe from harm property of Odontotermes formosanus, the mobility for being colored Odontotermes formosanus will not be reduced.
Specific embodiment
Further description of the technical solution of the present invention With reference to embodiment.
Obviously, the described embodiments are merely a part of the embodiments of the present invention, instead of all the embodiments.Based on this
Embodiment in invention, all other reality obtained by those of ordinary skill in the art without making creative efforts
Example is applied, shall fall within the protection scope of the present invention.
Embodiment 1
A kind of Odontotermes formosanus colouring method, comprising the following steps:
A) coloring agent is prepared: taking distilled water, neutral red dye and rhodamine, the rhodamine for being configured to 0.1wt% respectively molten
The dimethyl diaminophenazine chloride dye solution of liquid and 0.8wt%；Then dimethyl diaminophenazine chloride dye solution and rhodamine liquor are mixed by the weight ratio of 1:0.5
It closes, coloring agent is made；
B) phagostimulant is prepared: weighing 10 parts of millet flours, 3 parts of cornstarch, 1 part of pollen pini, 5 parts of pine powders, 4 by weight
Part masson pine bark, 2 portions of bagasse and 5 portions of sucrose, are dried 30 minutes at 30 DEG C after above-mentioned raw materials are mixed, are then crushed to
Phagostimulant is made in 80 mesh；
C) it dyes bait preparing: phagostimulant is mixed with coloring agent by the weight ratio of 1:1.1, filter off liquid simultaneously after 3 minutes
Dyeing bait is made in drying and processing 30 minutes at 60 DEG C；
D) dyeing bait is laid in culture dish, then launches the Odontotermes formosanus of crawl thereto, and spray thereto
Appropriate distilled water makes the relative humidity in culture dish be maintained at 80%, closes the lid, cultivates in darkroom, and keeping temperature is 15
℃；Before dyeing bait is laid in culture dish, the quartz sand for one layer of 14 mesh that first tile in culture dish, the quartz sand is before laying
First through sterilization processing；
E) Odontotermes formosanus is cultivated 3 days, it is daily as wherein spraying appropriate distilled water, keep the relative humidity in culture dish
80%；
F) after the completion of dyeing culture, the Odontotermes formosanus of dyed label is removed.
Embodiment 2
A kind of Odontotermes formosanus colouring method, comprising the following steps:
A) coloring agent is prepared: being taken distilled water, neutral red dye and rhodamine B, is configured to the rhodamine B of 0.2wt% respectively
The dimethyl diaminophenazine chloride dye solution of solution and 1.0wt%；Then dimethyl diaminophenazine chloride dye solution and rhodamine B solution are pressed to the weight ratio of 1:1
Coloring agent is made in mixing；
B) phagostimulant is prepared: weighing 10 parts of millet flours, 4 parts of cornstarch, 1.5 parts of pollen pinis, 6 portions of pines by weight
Powder, 5 parts of camphorwood skins, 3 portions of bagasse and 6 portions of sucrose, are dried 35 minutes at 35 DEG C after above-mentioned raw materials are mixed, then powder
90 mesh are broken to, phagostimulant is made；
C) it dyes bait preparing: phagostimulant is mixed with coloring agent by the weight ratio of 1:1.2, filter off liquid simultaneously after 4 minutes
Dyeing bait is made in drying and processing 40 minutes at 65 DEG C；
D) dyeing bait is laid in culture dish, then launches the Odontotermes formosanus of crawl thereto, and spray thereto
Appropriate distilled water makes the relative humidity in culture dish be maintained at 82%, closes the lid, cultivates in darkroom, and keeping temperature is 20
℃；Before dyeing bait is laid in culture dish, the quartz sand for one layer of 15 mesh that first tile in culture dish, the quartz sand is before laying
First through sterilization processing；
E) Odontotermes formosanus is cultivated 4 days, it is daily as wherein spraying appropriate distilled water, keep the relative humidity in culture dish
82%；
F) after the completion of dyeing culture, the Odontotermes formosanus of dyed label is removed.
Embodiment 3
A kind of Odontotermes formosanus colouring method, comprising the following steps:
A) coloring agent is prepared: taking distilled water, alkaline red dye and rhodamine, the rhodamine for being configured to 0.4wt% respectively molten
The alkaline red dye solution of liquid and 2.0wt%；Then alkaline red dye solution and rhodamine liquor are mixed by the weight ratio of 1:1.5
It closes, coloring agent is made；
B) phagostimulant is prepared: weighing 10 parts of millet flours, 4 parts of cornstarch, 1.5 parts of pollen pinis, 6 parts of camphorwoods by weight
Powder, 7 parts of masson pine barks, 4 portions of bagasse and 7 portions of sucrose, are dried 35 minutes at 40 DEG C after above-mentioned raw materials are mixed, then powder
110 mesh are broken to, phagostimulant is made；
C) it dyes bait preparing: phagostimulant is mixed with coloring agent by the weight ratio of 1:1.4, filter off liquid simultaneously after 4 minutes
Dyeing bait is made in drying and processing 50 minutes at 65 DEG C；
D) dyeing bait is laid in culture dish, then launches the Odontotermes formosanus of crawl thereto, and spray thereto
Appropriate distilled water makes the relative humidity in culture dish be maintained at 83%, closes the lid, cultivates in darkroom, and keeping temperature is 25
℃；Before dyeing bait is laid in culture dish, the quartz sand for one layer of 15 mesh that first tile in culture dish, the quartz sand is before laying
First through sterilization processing；
E) Odontotermes formosanus is cultivated 6 days, it is daily as wherein spraying appropriate distilled water, keep the relative humidity in culture dish
83%；
F) after the completion of dyeing culture, the Odontotermes formosanus of dyed label is removed.
Embodiment 4
A kind of Odontotermes formosanus colouring method, comprising the following steps:
A) coloring agent is prepared: being taken distilled water, alkaline red dye and rhodamine B, is configured to the rhodamine B of 0.5wt% respectively
The alkaline red dye solution of solution and 2.4wt%；Then alkaline red dye solution and rhodamine B solution are pressed to the weight ratio of 1:2
Coloring agent is made in mixing；
B) phagostimulant is prepared: weighing 10 parts of millet flours, 5 parts of cornstarch, 2 parts of pollen pinis, 7 parts of camphorwood powders, 9 by weight
Part camphorwood skin, 5 portions of bagasse and 8 portions of sucrose, are dried 40 minutes at 45 DEG C after above-mentioned raw materials are mixed, are then crushed to
Phagostimulant is made in 120 mesh；
C) it dyes bait preparing: phagostimulant is mixed with coloring agent by the weight ratio of 1:1.5, filter off liquid simultaneously after five minutes
Dyeing bait is made in drying and processing 60 minutes at 70 DEG C；
D) dyeing bait is laid in culture dish, then launches the Odontotermes formosanus of crawl thereto, and spray thereto
Appropriate distilled water makes the relative humidity in culture dish be maintained at 85%, closes the lid, cultivates in darkroom, and keeping temperature is 30
℃；Before dyeing bait is laid in culture dish, the quartz sand for one layer of 16 mesh that first tile in culture dish, the quartz sand is before laying
First through sterilization processing；
E) Odontotermes formosanus is cultivated 7 days, it is daily as wherein spraying appropriate distilled water, keep the relative humidity in culture dish
80~85%；
F) after the completion of dyeing culture, the Odontotermes formosanus of dyed label is removed.
Comparative experiments and result:
Using above-mentioned Examples 1 to 4 as experimental group 1~4, the filter paper of neutral red solution was impregnated as control group A to feed, with
Conventional dyes spray coating method is labeled as control group B, observes dyeing effect and the influence to dyeing to Odontotermes formosanus.
It should be understood that those skilled in the art, can be improved or be become according to the above description
It changes, and all these modifications and variations should all belong to the protection domain of appended claims of the present invention.
Claims (4)
1. a kind of Odontotermes formosanus colouring method, it is characterised in that the following steps are included:
A) coloring agent is prepared: being taken distilled water, water-soluble dye and tagged fluorescent agent, is configured to the fluorescence of 0.1~0.5wt% respectively
The dye solution of label solution and 0.8~2.4wt%；Then dye solution and fluorescent marker solution are pressed to the weight of 1:0.5~2
Than mixing, coloring agent is made；The water-soluble dye is one of neutral red dye or alkaline red dye；Described is glimmering
Signal agent is one of rhodamine or rhodamine B；
B) phagostimulant is prepared: weighing 10 parts of millet flours, 3~5 parts of cornstarch, 1~2 part of pollen pini, 5~7 parts of wood by weight
Powder, 4~9 parts of dry barks, 2~5 portions of bagasse and 5~8 portions of sucrose, are dried at 30~45 DEG C after above-mentioned raw materials are mixed
30~40 minutes, 80~120 mesh are then crushed to, phagostimulant is made；
C) it dyes bait preparing: phagostimulant is mixed with coloring agent by the weight ratio of 1:1.1~1.5,3~liquid is filtered off after five minutes
Dyeing bait is made in body and drying and processing 30~60 minutes at 60~70 DEG C；
D) dyeing bait is laid in culture dish, then launches the Odontotermes formosanus of crawl thereto, and spray is appropriate thereto
Distilled water makes the relative humidity in culture dish be maintained at 80~85%, closes the lid, cultivates in darkroom, keep temperature be 15~
30℃；
E) Odontotermes formosanus is cultivated 3~7 days, it is daily as wherein spraying appropriate distilled water, keep the relative humidity in culture dish
80~85%；
F) after the completion of dyeing culture, the Odontotermes formosanus of dyed label is removed.
2. a kind of Odontotermes formosanus colouring method according to claim 1, it is characterised in that: the wood powder is pine powder
Perhaps the camphorwood powder dry bark is masson pine bark or camphorwood skin.
3. a kind of Odontotermes formosanus colouring method according to claim 1 or 2, it is characterised in that: the wood powder is pine
Wood powder, the dry bark are camphorwood skin.
4. a kind of Odontotermes formosanus colouring method according to claim 1, it is characterised in that: in the step d, dye bait
Before material is laid in culture dish, the quartz sand for one layer of 14~16 mesh that first tile in culture dish, the quartz sand is first passed through before laying
Sterilization processing.
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| CN107996516B (en) * | 2017-11-16 | 2023-04-11 | 中国农业科学院蜜蜂研究所 | Automatic bee marking device and using method thereof |
| CN115669688B (en) * | 2023-01-05 | 2023-04-07 | 青岛市房屋使用安全服务中心(青岛市白蚁防治研究所) | Termite bait |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103975969A (en) * | 2014-05-30 | 2014-08-13 | 湖北省森林病虫害防治检疫总站 | Termite attractive toxicant and preparation method thereof |
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| CN105409888A (en) * | 2015-10-30 | 2016-03-23 | 成都市白蚁防治研究所 | Method for marking termites by staining |
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| CN105265395A (en) * | 2015-10-30 | 2016-01-27 | 成都市白蚁防治研究所 | Multifunctional termite separator |
| CN105409888A (en) * | 2015-10-30 | 2016-03-23 | 成都市白蚁防治研究所 | Method for marking termites by staining |
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