CN107045028A - A kind of place of production mixing Chinese prickly ash content authentication method of Zhengzhou Hanyuan two - Google Patents
A kind of place of production mixing Chinese prickly ash content authentication method of Zhengzhou Hanyuan two Download PDFInfo
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- CN107045028A CN107045028A CN201710213315.8A CN201710213315A CN107045028A CN 107045028 A CN107045028 A CN 107045028A CN 201710213315 A CN201710213315 A CN 201710213315A CN 107045028 A CN107045028 A CN 107045028A
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- prickly ash
- chinese prickly
- zhengzhou
- hanyuan
- authentication method
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8624—Detection of slopes or peaks; baseline correction
- G01N30/8631—Peaks
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of place of production mixing Chinese prickly ash content authentication method of Zhengzhou Hanyuan two, Zhengzhou and Hanyuan Chinese prickly ash price variance are larger, and the Chinese prickly ash Adulteration identification of Chinese prickly ash document report can only be qualitative, can determine whether adulterated, but it can not quantify, i.e., not can determine that mixed how many.The present invention is characterized ion with m/z133 and is characterized ion with m/z 161, the ratio of Zhengzhou Chinese prickly ash and Hanyuan Chinese prickly ash after being mixed with quantitative analysis.The present invention, which has, does not need standard substance, simple to operate, the features such as testing cost, with very wide application.
Description
Technical field
The present invention relates to a kind of food adulteration content analysis field, and in particular to a kind of place of production mixing Chinese prickly ash of Zhengzhou Hanyuan two
Content authentication method.
Background technology
Chinese prickly ash have adjustment stomach, relieve summer heat, anti-inflammatory, diuresis, analgesic, it is deep to be liked by compatriots while mouthfeel is unique, be house
Indispensable flavouring, therefore, the cultivated area of Chinese prickly ash are wide, and yield is big, but different sources Chinese prickly ash profile is similar, and price is irregular not
Together.Some illegal retailers in varing proportions mix the Chinese prickly ash of different sources, fill excellent with bad.At present, Zhengzhou and Hanyuan Chinese prickly ash valency
Lattice differ greatly, and the Chinese prickly ash Adulteration identification of Chinese prickly ash document report can only be qualitative, i.e., by analysis, be capable of determining whether it is adulterated, but
It can not quantify, i.e., not can determine that mixed how many.The present invention passes through HS-SPME-GC/MS (Headspace-solid phase microextractions-gas-chromatography
With mass spectrometry) analysis, the ratio of Zhengzhou Chinese prickly ash and Hanyuan Chinese prickly ash after being mixed using characteristic ion quantitative analysis.
The content of the invention
Spent it is an object of the present invention to overcome the deficiencies in the prior art there is provided a kind of mixing of place of production of Zhengzhou Hanyuan two
Green pepper content authentication method.
The present invention is achieved through the following technical solutions：
1 Zhengzhou and the former Chinese prickly ash of the Chinese are some.
2 Zhengzhou Chinese prickly ash contents are 0%, 20%, 40%, 60%, 80%, 100% mixing.
3 weigh different proportion each 0.5g of mixed Chinese prickly ash respectively, are put into 15mL ml headspace bottles, add 5mL distilled water.
4 60 DEG C of water-baths, ml headspace bottle balance 30min, 30min is adsorbed using ACAR/PDMS/DVB solid-phase extraction columns.
5 extraction columns are in 250 DEG C of vaporizer desorption 3min of gas-chromatography.
7 use WAX capillary chromatographic columns, and heating schedule is 50 DEG C of initial temperature, and 5 DEG C/min is raised to 100 DEG C, 2 DEG C/min
110 DEG C are raised to, 5 DEG C/min is raised to 150 DEG C, and 4 DEG C/min is raised to 180 DEG C, keeps 10min.
Mass Spectrometry Conditions：EI ion guns, 230 DEG C of ion source temperature, 150 DEG C of level Four bar temperature, 250 DEG C of interface temperature, electronics
Energy 70eV, solvent delay 6.5min, scan mode scan, mass range 29-700amu.
8 are characterized ion with m/z133, extract ion flow graph, obtain the peak that retention time is 28.62min-28.71min
Area S1.
9 are characterized ion with m/z 161, extract ion flow graph, obtain the peak that retention time is 26.72min-26.82min
Area S2.
10 with S1/ (S1+S2) be ordinate, abscissa is mixed into varing proportions and does figure.Obtain matched curve.
11, for unknown mixed proportion Zhengzhou Hanyuan Chinese prickly ash, take 0.5g, are put into 15mL ml headspace bottles, by 4-9 steps, survey
S1/ (S1+S2) ratio, bring into matched curve, you can calculate the unknown mixed proportion of Zhengzhou Hanyuan Chinese prickly ash.
Brief description of the drawings
Accompanying drawing is the matched curve in embodiment 1.
Embodiment：
Technical scheme is described further below by specific embodiment：
Embodiment 1
1 Zhengzhou and the former Chinese prickly ash of the Chinese are some.
2 Zhengzhou Chinese prickly ash contents are 0%, 20%, 40%, 60%, 80%, 100% mixing.
3 weigh the mixed Chinese prickly ash of different proportion respectively, and each 0.5g of the Chinese prickly ash in Zhengzhou and Hanyuan is put into 15mL ml headspace bottles
In.
4 60 DEG C of water-baths, ml headspace bottle balance 30min, 30min is adsorbed using ACAR/PDMS/DVB solid-phase extraction columns.
5 extraction columns are in 250 DEG C of vaporizer desorption 3min of gas-chromatography.
7 use WAX capillary chromatographic columns, and heating schedule is 50 DEG C of initial temperature, and 5 DEG C/min is raised to 100 DEG C, 2 DEG C/min
110 DEG C are raised to, 5 DEG C/min is raised to 150 DEG C, and 4 DEG C/min is raised to 180 DEG C, keeps 10min.
Mass Spectrometry Conditions：EI ion guns, 230 DEG C of ion source temperature, 150 DEG C of level Four bar temperature, 250 DEG C of interface temperature, electronics
Energy 70eV, solvent delay 6.5min, scan mode scan, mass range 29-700amu.
8 are characterized ion with m/z133, extract ion flow graph, obtain the peak that retention time is 28.62min-28.71min
Area S1.
9 are characterized ion with m/z 161, extract ion flow graph, obtain the peak that retention time is 26.72min-26.82min
Area S2.
10 with S1/ (S1+S2) be ordinate, abscissa is mixed into varing proportions and does figure, such as scheme.Obtain matched curve y
=1.0348x3-1.2338x2+0.8644x+0.2656
This method blind sample (Zhengzhou Chinese prickly ash：Hanyuan Chinese prickly ash is 3: Chinese prickly ash sample test 7) mixed, SPME is carried out,
GC-MS is analyzed, and it is 0.444 to obtain S1/ (S1+S2) value, and the mixed proportion calculated by matched curve is 0.306.Deviation ratio is
2%.
Claims (1)
1. a kind of place of production mixing Chinese prickly ash content authentication method of Zhengzhou Hanyuan two, it is characterised in that：Ion is characterized with m/z133, carried
Ion flow graph is taken, the peak area S1 that retention time is 28.62min-28.71min is obtained.Ion is characterized with m/z161, extracted
Ion flow graph, obtains the peak area S2 that retention time is 26.72min-26.82min.With S1/ (S1+S2) for ordinate, with not
Abscissa is mixed into proportion does figure.Obtain matched curve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710213315.8A CN107045028A (en) | 2017-03-27 | 2017-03-27 | A kind of place of production mixing Chinese prickly ash content authentication method of Zhengzhou Hanyuan two |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710213315.8A CN107045028A (en) | 2017-03-27 | 2017-03-27 | A kind of place of production mixing Chinese prickly ash content authentication method of Zhengzhou Hanyuan two |
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| Publication Number | Publication Date |
|---|---|
| CN107045028A true CN107045028A (en) | 2017-08-15 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710213315.8A Pending CN107045028A (en) | 2017-03-27 | 2017-03-27 | A kind of place of production mixing Chinese prickly ash content authentication method of Zhengzhou Hanyuan two |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116338020A (en) * | 2022-10-26 | 2023-06-27 | 西北农林科技大学 | Method for detecting volatile terpenoid in pepper fruits and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102749400A (en) * | 2012-07-26 | 2012-10-24 | 天津春发生物科技集团有限公司 | Method for constructing HPLC-DAD (High Performance Liquid Chromatography-Diode Array Detector) fingerprint chromatography of zanthoxylum oil |
| CN104931605A (en) * | 2015-05-20 | 2015-09-23 | 增城华栋调味品有限公司 | Method for detecting quality of pepper powder based on component analysis |
-
2017
- 2017-03-27 CN CN201710213315.8A patent/CN107045028A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102749400A (en) * | 2012-07-26 | 2012-10-24 | 天津春发生物科技集团有限公司 | Method for constructing HPLC-DAD (High Performance Liquid Chromatography-Diode Array Detector) fingerprint chromatography of zanthoxylum oil |
| CN104931605A (en) * | 2015-05-20 | 2015-09-23 | 增城华栋调味品有限公司 | Method for detecting quality of pepper powder based on component analysis |
Non-Patent Citations (3)
| Title |
|---|
| 杨静等: "青花椒香气快速气相电子鼻响应特征及GC-MS物质基础分析", 《食品科学》 * |
| 陈光静等: "不同产地红花椒挥发油化学成分的比较研究", 《中国粮油学报》 * |
| 马利杰等: "基于电子鼻技术对羊奶粉中掺假牛奶粉的快速检测", 《中国乳品工业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116338020A (en) * | 2022-10-26 | 2023-06-27 | 西北农林科技大学 | Method for detecting volatile terpenoid in pepper fruits and application thereof |
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Application publication date: 20170815 |
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| RJ01 | Rejection of invention patent application after publication |